LOCUS       DACCIQ010000001        96574 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCIQ010000001 DACCIQ010000000
VERSION     DACCIQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 96574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.001"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVM62_000001"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVM62_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP6843997.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVM62_000002"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVM62_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP6843998.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVM62_000003"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVM62_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP6843999.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVM62_000004"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVM62_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP6844000.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVM62_000005"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVM62_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP6844001.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVM62_000006"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVM62_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP6844002.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVM62_000007"
     CDS             complement(8053..8523)
                     /locus_tag="IVM62_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP6844003.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVM62_000008"
     CDS             complement(8569..8994)
                     /locus_tag="IVM62_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP6844004.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVM62_000009"
     CDS             complement(9061..9330)
                     /locus_tag="IVM62_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP6844005.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVM62_000010"
     CDS             complement(9341..10936)
                     /locus_tag="IVM62_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6844006.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVM62_000011"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVM62_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP6844007.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVM62_000012"
     CDS             complement(14489..15049)
                     /locus_tag="IVM62_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP6844008.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVM62_000013"
     CDS             15402..16376
                     /locus_tag="IVM62_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6844009.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVM62_000014"
     CDS             16546..17946
                     /locus_tag="IVM62_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP6844010.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVM62_000015"
     CDS             complement(18024..18746)
                     /locus_tag="IVM62_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6844011.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVM62_000016"
     CDS             complement(18825..19331)
                     /locus_tag="IVM62_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6844012.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVM62_000017"
     CDS             19454..21253
                     /locus_tag="IVM62_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP6844013.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IVM62_000018"
     CDS             complement(21321..22895)
                     /locus_tag="IVM62_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844014.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVM62_000019"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IVM62_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP6844015.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVM62_000020"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IVM62_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP6844016.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IVM62_000021"
     CDS             complement(24672..25310)
                     /locus_tag="IVM62_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP6844017.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IVM62_000022"
     CDS             complement(25311..25961)
                     /locus_tag="IVM62_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6844018.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVM62_000023"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IVM62_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP6844019.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVM62_000024"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IVM62_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP6844020.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IVM62_000025"
     CDS             complement(29104..29844)
                     /locus_tag="IVM62_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP6844021.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVM62_000026"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IVM62_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP6844022.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IVM62_000027"
     CDS             complement(31215..32153)
                     /locus_tag="IVM62_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP6844023.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVM62_000028"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IVM62_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6844024.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVM62_000029"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IVM62_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP6844025.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IVM62_000030"
     CDS             complement(35313..35618)
                     /locus_tag="IVM62_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6844026.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVM62_000031"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IVM62_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP6844027.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IVM62_000032"
     CDS             complement(36724..37008)
                     /locus_tag="IVM62_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844028.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IVM62_000033"
     CDS             complement(37026..37916)
                     /locus_tag="IVM62_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP6844029.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IVM62_000034"
     CDS             complement(37936..38154)
                     /locus_tag="IVM62_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844030.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IVM62_000035"
     CDS             complement(38124..39824)
                     /locus_tag="IVM62_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP6844031.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IVM62_000036"
     CDS             40091..40864
                     /locus_tag="IVM62_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844032.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IVM62_000037"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IVM62_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP6844033.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IVM62_000038"
     CDS             complement(41688..42659)
                     /locus_tag="IVM62_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6844034.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IVM62_000039"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IVM62_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP6844035.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IVM62_000040"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IVM62_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP6844036.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IVM62_000041"
     CDS             complement(45614..46438)
                     /locus_tag="IVM62_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6844037.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IVM62_000042"
     CDS             complement(46411..47091)
                     /locus_tag="IVM62_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6844038.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IVM62_000043"
     CDS             complement(47114..48052)
                     /locus_tag="IVM62_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP6844039.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IVM62_000044"
     CDS             complement(48297..49148)
                     /locus_tag="IVM62_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP6844040.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IVM62_000045"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IVM62_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6844041.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IVM62_000046"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IVM62_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP6844042.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IVM62_000047"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IVM62_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP6844043.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IVM62_000048"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IVM62_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP6844044.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IVM62_000049"
     CDS             complement(56026..57267)
                     /locus_tag="IVM62_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP6844045.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IVM62_000050"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IVM62_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP6844046.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IVM62_000051"
     CDS             complement(58294..59178)
                     /locus_tag="IVM62_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6844047.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IVM62_000052"
     CDS             complement(59956..61242)
                     /locus_tag="IVM62_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6844048.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IVM62_000053"
     CDS             complement(61235..62503)
                     /locus_tag="IVM62_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6844049.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IVM62_000054"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IVM62_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP6844050.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="IVM62_000055"
     CDS             complement(63275..63721)
                     /locus_tag="IVM62_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844051.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="IVM62_000056"
     CDS             complement(63814..64059)
                     /locus_tag="IVM62_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844052.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64174..64752)
                     /locus_tag="IVM62_000057"
     CDS             complement(64174..64752)
                     /locus_tag="IVM62_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6844053.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64829..65359)
                     /locus_tag="IVM62_000058"
     CDS             complement(64829..65359)
                     /locus_tag="IVM62_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP6844054.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IVM62_000059"
     CDS             complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IVM62_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP6844055.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66216..66611)
                     /locus_tag="IVM62_000060"
     CDS             complement(66216..66611)
                     /locus_tag="IVM62_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6844056.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66608..68020)
                     /locus_tag="IVM62_000061"
     CDS             complement(66608..68020)
                     /locus_tag="IVM62_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP6844057.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IVM62_000062"
     CDS             complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IVM62_000062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP6844058.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68875..70335)
                     /locus_tag="IVM62_000063"
     CDS             complement(68875..70335)
                     /locus_tag="IVM62_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP6844059.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70505..71662)
                     /locus_tag="IVM62_000064"
     CDS             complement(70505..71662)
                     /locus_tag="IVM62_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP6844060.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IVM62_000065"
     CDS             complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IVM62_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP6844061.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73129..73656)
                     /locus_tag="IVM62_000066"
     CDS             complement(73129..73656)
                     /locus_tag="IVM62_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP6844062.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73886..74692
                     /gene="proB"
                     /locus_tag="IVM62_000067"
     CDS             73886..74692
                     /gene="proB"
                     /locus_tag="IVM62_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP6844063.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74689..75936
                     /locus_tag="IVM62_000068"
     CDS             74689..75936
                     /locus_tag="IVM62_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP6844064.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76012..76356
                     /locus_tag="IVM62_000069"
     CDS             76012..76356
                     /locus_tag="IVM62_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844065.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76473..76799)
                     /locus_tag="IVM62_000070"
     CDS             complement(76473..76799)
                     /locus_tag="IVM62_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844066.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77414..78616)
                     /locus_tag="IVM62_000071"
     CDS             complement(77414..78616)
                     /locus_tag="IVM62_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6844067.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78733..79248
                     /locus_tag="IVM62_000072"
     CDS             78733..79248
                     /locus_tag="IVM62_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6844068.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79447..79893)
                     /locus_tag="IVM62_000073"
     CDS             complement(79447..79893)
                     /locus_tag="IVM62_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6844069.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IVM62_000074"
     CDS             complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IVM62_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6844070.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81772..82401)
                     /locus_tag="IVM62_000075"
     CDS             complement(81772..82401)
                     /locus_tag="IVM62_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6844071.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82385..82849)
                     /locus_tag="IVM62_000076"
     CDS             complement(82385..82849)
                     /locus_tag="IVM62_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6844072.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83336..84604)
                     /locus_tag="IVM62_000077"
     CDS             complement(83336..84604)
                     /locus_tag="IVM62_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6844073.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84835..85572)
                     /locus_tag="IVM62_000078"
     CDS             complement(84835..85572)
                     /locus_tag="IVM62_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6844074.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85639..86208)
                     /locus_tag="IVM62_000079"
     CDS             complement(85639..86208)
                     /locus_tag="IVM62_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6844075.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86336..87208
                     /locus_tag="IVM62_000080"
     CDS             86336..87208
                     /locus_tag="IVM62_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6844076.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87550..88422
                     /locus_tag="IVM62_000081"
     CDS             87550..88422
                     /locus_tag="IVM62_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6844077.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88419..90377
                     /locus_tag="IVM62_000082"
     CDS             88419..90377
                     /locus_tag="IVM62_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6844078.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90709..91560)
                     /locus_tag="IVM62_000083"
     CDS             complement(90709..91560)
                     /locus_tag="IVM62_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6844079.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91683..92150
                     /locus_tag="IVM62_000084"
     CDS             91683..92150
                     /locus_tag="IVM62_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6844080.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92413..92895)
                     /locus_tag="IVM62_000085"
     CDS             complement(92413..92895)
                     /locus_tag="IVM62_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6844081.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92995..93858)
                     /locus_tag="IVM62_000086"
     CDS             complement(92995..93858)
                     /locus_tag="IVM62_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6844082.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94456..95331
                     /locus_tag="IVM62_000087"
     CDS             94456..95331
                     /locus_tag="IVM62_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844083.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(95476..95628)
                     /locus_tag="IVM62_000088"
                     /pseudo
     CDS             complement(95476..95628)
                     /locus_tag="IVM62_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(95896..>96574)
                     /locus_tag="IVM62_000089"
     CDS             complement(95896..>96574)
                     /locus_tag="IVM62_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6844084.1"
                     /translation="NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQA
                     VISGSLLDPFLIQNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFI
                     FSRRKDYKDFAKLSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATA
                     VGLINHVVVQIPWTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTT
                     KLSENET"
BASE COUNT        27155 a        20716 c        17296 g        31407 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64201 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64261 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64321 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64381 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64441 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    64501 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    64561 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    64621 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    64681 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    64741 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    64801 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    64861 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    64921 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    64981 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65041 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65101 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65161 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65221 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65281 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65341 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65401 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    65461 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    65521 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    65581 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    65641 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    65701 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    65761 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    65821 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    65881 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    65941 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66001 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66061 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66121 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66181 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66241 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66301 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66361 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66421 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    66481 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    66541 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    66601 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    66661 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    66721 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    66781 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    66841 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    66901 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    66961 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67021 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67081 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67141 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67201 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67261 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67321 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67381 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67441 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    67501 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    67561 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    67621 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    67681 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    67741 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    67801 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    67861 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    67921 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    67981 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68041 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68101 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68161 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68221 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68281 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68341 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68401 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    68461 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    68521 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    68581 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    68641 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    68701 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    68761 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    68821 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    68881 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    68941 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69001 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69061 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69121 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69181 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
    69241 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69301 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69361 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69421 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    69481 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    69541 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    69601 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    69661 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    69721 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    69781 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    69841 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    69901 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    69961 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70021 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70081 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70141 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70201 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70261 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70321 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70381 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70441 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    70501 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    70561 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    70621 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    70681 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    70741 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    70801 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    70861 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    70921 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    70981 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71041 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71101 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71161 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71221 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71281 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71341 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71401 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    71461 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    71521 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    71581 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    71641 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    71701 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    71761 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    71821 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    71881 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    71941 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72001 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72061 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72121 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72181 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72241 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72301 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72361 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72421 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    72481 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    72541 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    72601 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    72661 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    72721 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    72781 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    72841 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    72901 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    72961 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73021 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73081 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73141 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73201 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73261 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73321 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73381 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73441 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    73501 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    73561 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    73621 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    73681 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    73741 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    73801 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    73861 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    73921 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    73981 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74041 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74101 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74161 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74221 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74281 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74341 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74401 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    74461 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    74521 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    74581 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    74641 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    74701 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    74761 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    74821 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    74881 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    74941 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75001 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75061 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75121 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75181 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75241 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75301 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75361 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75421 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    75481 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    75541 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    75601 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    75661 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    75721 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    75781 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    75841 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    75901 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    75961 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76021 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76081 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    76141 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76201 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76261 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76321 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76381 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76441 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    76501 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    76561 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    76621 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    76681 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    76741 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    76801 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    76861 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    76921 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    76981 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77041 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77101 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77161 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77221 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77281 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77341 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77401 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    77461 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    77521 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    77581 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    77641 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    77701 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    77761 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    77821 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    77881 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    77941 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78001 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78061 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78121 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78181 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78241 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78301 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78361 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78421 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    78481 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    78541 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    78601 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    78661 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    78721 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    78781 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    78841 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    78901 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    78961 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79021 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79081 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79141 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79201 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79261 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79321 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79381 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79441 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    79501 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    79561 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    79621 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    79681 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    79741 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    79801 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    79861 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    79921 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    79981 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80041 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80101 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80161 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80221 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80281 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80341 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80401 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    80461 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    80521 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    80581 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    80641 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    80701 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    80761 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    80821 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    80881 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    80941 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81001 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81061 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81121 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81181 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81241 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81301 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81361 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81421 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    81481 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    81541 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    81601 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    81661 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    81721 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    81781 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    81841 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    81901 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    81961 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82021 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82081 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82141 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82201 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82261 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82321 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82381 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82441 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    82501 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    82561 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    82621 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    82681 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    82741 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    82801 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    82861 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    82921 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    82981 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83041 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83101 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83161 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83221 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83281 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83341 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83401 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    83461 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    83521 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    83581 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    83641 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    83701 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    83761 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    83821 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    83881 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    83941 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84001 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84061 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84121 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84181 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84241 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84301 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84361 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84421 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    84481 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    84541 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    84601 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    84661 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    84721 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    84781 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    84841 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    84901 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    84961 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85021 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85081 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85141 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85201 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85261 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85321 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85381 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85441 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    85501 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    85561 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    85621 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    85681 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    85741 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    85801 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    85861 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    85921 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    85981 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    86041 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    86101 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    86161 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    86221 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    86281 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    86341 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    86401 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    86461 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    86521 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    86581 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    86641 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    86701 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    86761 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    86821 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    86881 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    86941 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    87001 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    87061 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    87121 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    87181 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    87241 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    87301 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    87361 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    87421 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    87481 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    87541 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    87601 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    87661 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    87721 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    87781 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    87841 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    87901 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    87961 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    88021 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    88081 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    88141 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    88201 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    88261 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    88321 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    88381 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    88441 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    88501 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    88561 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    88621 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    88681 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    88741 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    88801 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    88861 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    88921 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    88981 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    89041 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    89101 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    89161 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    89221 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    89281 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    89341 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    89401 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    89461 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    89521 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    89581 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    89641 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    89701 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    89761 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    89821 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    89881 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    89941 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    90001 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    90061 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    90121 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    90181 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    90241 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    90301 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    90361 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    90421 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    90481 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    90541 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    90601 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    90661 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    90721 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    90781 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    90841 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    90901 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    90961 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    91021 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    91081 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    91141 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    91201 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    91261 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    91321 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    91381 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    91441 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    91501 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    91561 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    91621 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    91681 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    91741 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    91801 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    91861 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    91921 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    91981 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    92041 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    92101 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    92161 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    92221 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    92281 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    92341 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    92401 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    92461 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    92521 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    92581 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    92641 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    92701 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    92761 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    92821 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    92881 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    92941 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    93001 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    93061 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    93121 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    93181 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    93241 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    93301 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    93361 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    93421 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    93481 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    93541 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    93601 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    93661 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    93721 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    93781 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    93841 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    93901 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    93961 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    94021 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    94081 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    94141 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    94201 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    94261 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    94321 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    94381 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    94441 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    94501 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    94561 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    94621 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    94681 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    94741 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    94801 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    94861 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    94921 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    94981 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    95041 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    95101 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    95161 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    95221 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    95281 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    95341 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    95401 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    95461 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    95521 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    95581 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    95641 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    95701 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    95761 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    95821 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    95881 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    95941 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    96001 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    96061 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    96121 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    96181 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    96241 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    96301 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    96361 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    96421 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    96481 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    96541 tatcgtggta attagcttat ttatatccat atta
//
LOCUS       DACCIQ010000002        84798 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCIQ010000002 DACCIQ010000000
VERSION     DACCIQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 84798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.002"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            41..922
                     /locus_tag="IVM62_000090"
     CDS             41..922
                     /locus_tag="IVM62_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844085.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="IVM62_000091"
     CDS             935..2545
                     /locus_tag="IVM62_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP6844086.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="IVM62_000092"
     CDS             complement(2622..3281)
                     /locus_tag="IVM62_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6844087.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="IVM62_000093"
     CDS             complement(3564..4229)
                     /locus_tag="IVM62_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844088.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="IVM62_000094"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="IVM62_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP6844089.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..5992)
                     /gene="dhaK"
                     /locus_tag="IVM62_000095"
     CDS             complement(5009..5992)
                     /gene="dhaK"
                     /locus_tag="IVM62_000095"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP6844090.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5994..6389)
                     /gene="dhaM"
                     /locus_tag="IVM62_000096"
     CDS             complement(5994..6389)
                     /gene="dhaM"
                     /locus_tag="IVM62_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP6844091.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="IVM62_000097"
     CDS             6543..6863
                     /locus_tag="IVM62_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP6844092.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7844)
                     /locus_tag="IVM62_000098"
     CDS             complement(6975..7844)
                     /locus_tag="IVM62_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6844093.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7940..9244)
                     /locus_tag="IVM62_000099"
     CDS             complement(7940..9244)
                     /locus_tag="IVM62_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6844094.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9241..9750)
                     /locus_tag="IVM62_000100"
     CDS             complement(9241..9750)
                     /locus_tag="IVM62_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844095.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9925..12060)
                     /locus_tag="IVM62_000101"
     CDS             complement(9925..12060)
                     /locus_tag="IVM62_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6844096.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12143..13798)
                     /locus_tag="IVM62_000102"
     CDS             complement(12143..13798)
                     /locus_tag="IVM62_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6844097.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13800..14114)
                     /locus_tag="IVM62_000103"
     CDS             complement(13800..14114)
                     /locus_tag="IVM62_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6844098.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14284..14544
                     /locus_tag="IVM62_000104"
     CDS             14284..14544
                     /locus_tag="IVM62_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP6844099.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14558..14872
                     /locus_tag="IVM62_000105"
     CDS             14558..14872
                     /locus_tag="IVM62_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP6844100.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15602..16156)
                     /locus_tag="IVM62_000106"
     CDS             complement(15602..16156)
                     /locus_tag="IVM62_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP6844101.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16282..18210)
                     /locus_tag="IVM62_000107"
     CDS             complement(16282..18210)
                     /locus_tag="IVM62_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6844102.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18516..19232)
                     /locus_tag="IVM62_000108"
     CDS             complement(18516..19232)
                     /locus_tag="IVM62_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6844103.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19233..21056)
                     /gene="glpO"
                     /locus_tag="IVM62_000109"
     CDS             complement(19233..21056)
                     /gene="glpO"
                     /locus_tag="IVM62_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP6844104.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21070..22566)
                     /gene="glpK"
                     /locus_tag="IVM62_000110"
     CDS             complement(21070..22566)
                     /gene="glpK"
                     /locus_tag="IVM62_000110"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP6844105.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22702..24162)
                     /locus_tag="IVM62_000111"
     CDS             complement(22702..24162)
                     /locus_tag="IVM62_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6844106.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24152..25447)
                     /locus_tag="IVM62_000112"
     CDS             complement(24152..25447)
                     /locus_tag="IVM62_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6844107.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25779..26456)
                     /locus_tag="IVM62_000113"
     CDS             complement(25779..26456)
                     /locus_tag="IVM62_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP6844108.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26486..27577)
                     /locus_tag="IVM62_000114"
     CDS             complement(26486..27577)
                     /locus_tag="IVM62_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP6844109.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27678..28112)
                     /locus_tag="IVM62_000115"
     CDS             complement(27678..28112)
                     /locus_tag="IVM62_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6844110.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28696..30153)
                     /gene="ascB"
                     /locus_tag="IVM62_000116"
     CDS             complement(28696..30153)
                     /gene="ascB"
                     /locus_tag="IVM62_000116"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6844111.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30150..32045)
                     /locus_tag="IVM62_000117"
     CDS             complement(30150..32045)
                     /locus_tag="IVM62_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6844112.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32103..33878)
                     /locus_tag="IVM62_000118"
     CDS             complement(32103..33878)
                     /locus_tag="IVM62_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6844113.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33928..35424)
                     /locus_tag="IVM62_000119"
     CDS             complement(33928..35424)
                     /locus_tag="IVM62_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6844114.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35618..37240)
                     /locus_tag="IVM62_000120"
     CDS             complement(35618..37240)
                     /locus_tag="IVM62_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6844115.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37689..39002)
                     /locus_tag="IVM62_000121"
     CDS             complement(37689..39002)
                     /locus_tag="IVM62_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6844116.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39247..39471)
                     /locus_tag="IVM62_000122"
     CDS             complement(39247..39471)
                     /locus_tag="IVM62_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844117.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39491..39859)
                     /locus_tag="IVM62_000123"
     CDS             complement(39491..39859)
                     /locus_tag="IVM62_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6844118.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(40056..40766)
                     /locus_tag="IVM62_000124"
     CDS             complement(40056..40766)
                     /locus_tag="IVM62_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844119.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40784..41281)
                     /locus_tag="IVM62_000125"
     CDS             complement(40784..41281)
                     /locus_tag="IVM62_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844120.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41318..41770)
                     /locus_tag="IVM62_000126"
     CDS             complement(41318..41770)
                     /locus_tag="IVM62_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6844121.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41905..42510)
                     /locus_tag="IVM62_000127"
     CDS             complement(41905..42510)
                     /locus_tag="IVM62_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6844122.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42960..43580)
                     /locus_tag="IVM62_000128"
     CDS             complement(42960..43580)
                     /locus_tag="IVM62_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6844123.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43582..44451)
                     /locus_tag="IVM62_000129"
     CDS             complement(43582..44451)
                     /locus_tag="IVM62_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP6844124.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44474..44881)
                     /locus_tag="IVM62_000130"
     CDS             complement(44474..44881)
                     /locus_tag="IVM62_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6844125.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44900..46189)
                     /locus_tag="IVM62_000131"
     CDS             complement(44900..46189)
                     /locus_tag="IVM62_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6844126.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46216..46518)
                     /locus_tag="IVM62_000132"
     CDS             complement(46216..46518)
                     /locus_tag="IVM62_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6844127.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46543..48636)
                     /locus_tag="IVM62_000133"
     CDS             complement(46543..48636)
                     /locus_tag="IVM62_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6844128.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49125..49889)
                     /locus_tag="IVM62_000134"
     CDS             complement(49125..49889)
                     /locus_tag="IVM62_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP6844129.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50000..50869)
                     /gene="mdcB"
                     /locus_tag="IVM62_000135"
     CDS             complement(50000..50869)
                     /gene="mdcB"
                     /locus_tag="IVM62_000135"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP6844130.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50894..51970)
                     /locus_tag="IVM62_000136"
     CDS             complement(50894..51970)
                     /locus_tag="IVM62_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP6844131.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51994..52587)
                     /locus_tag="IVM62_000137"
     CDS             complement(51994..52587)
                     /locus_tag="IVM62_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP6844132.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52591..54267)
                     /gene="mdcD"
                     /locus_tag="IVM62_000138"
     CDS             complement(52591..54267)
                     /gene="mdcD"
                     /locus_tag="IVM62_000138"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP6844133.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54257..54556)
                     /locus_tag="IVM62_000139"
     CDS             complement(54257..54556)
                     /locus_tag="IVM62_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP6844134.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54658..56304)
                     /gene="mdcA"
                     /locus_tag="IVM62_000140"
     CDS             complement(54658..56304)
                     /gene="mdcA"
                     /locus_tag="IVM62_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP6844135.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56311..57222)
                     /locus_tag="IVM62_000141"
     CDS             complement(56311..57222)
                     /locus_tag="IVM62_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6844136.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57342..58199
                     /locus_tag="IVM62_000142"
     CDS             57342..58199
                     /locus_tag="IVM62_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6844137.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58386..58748)
                     /locus_tag="IVM62_000143"
     CDS             complement(58386..58748)
                     /locus_tag="IVM62_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844138.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58937..59119)
                     /locus_tag="IVM62_000144"
     CDS             complement(58937..59119)
                     /locus_tag="IVM62_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844139.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59094..60533
                     /locus_tag="IVM62_000145"
     CDS             59094..60533
                     /locus_tag="IVM62_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6844140.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60540..63092)
                     /locus_tag="IVM62_000146"
     CDS             complement(60540..63092)
                     /locus_tag="IVM62_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP6844141.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63206..64537)
                     /locus_tag="IVM62_000147"
     CDS             complement(63206..64537)
                     /locus_tag="IVM62_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6844142.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64503..65981)
                     /locus_tag="IVM62_000148"
     CDS             complement(64503..65981)
                     /locus_tag="IVM62_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6844143.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66286..68121)
                     /gene="tdc"
                     /locus_tag="IVM62_000149"
     CDS             complement(66286..68121)
                     /gene="tdc"
                     /locus_tag="IVM62_000149"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6844144.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            68591..70018
                     /locus_tag="IVM62_000150"
     CDS             68591..70018
                     /locus_tag="IVM62_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6844145.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(70110..70475)
                     /locus_tag="IVM62_000151"
     CDS             complement(70110..70475)
                     /locus_tag="IVM62_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6844146.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(70472..70837)
                     /locus_tag="IVM62_000152"
     CDS             complement(70472..70837)
                     /locus_tag="IVM62_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6844147.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(71029..71277)
                     /locus_tag="IVM62_000153"
     CDS             complement(71029..71277)
                     /locus_tag="IVM62_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844148.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            71276..71404
                     /locus_tag="IVM62_000154"
     CDS             71276..71404
                     /locus_tag="IVM62_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP6844149.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            71461..73155
                     /locus_tag="IVM62_000155"
     CDS             71461..73155
                     /locus_tag="IVM62_000155"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP6844150.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            73222..73428
                     /locus_tag="IVM62_000156"
     CDS             73222..73428
                     /locus_tag="IVM62_000156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844151.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            73806..74939
                     /locus_tag="IVM62_000157"
     CDS             73806..74939
                     /locus_tag="IVM62_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP6844152.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            74970..75290
                     /locus_tag="IVM62_000158"
     CDS             74970..75290
                     /locus_tag="IVM62_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844153.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(75319..75783)
                     /locus_tag="IVM62_000159"
     CDS             complement(75319..75783)
                     /locus_tag="IVM62_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844154.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(75915..77168)
                     /locus_tag="IVM62_000160"
     CDS             complement(75915..77168)
                     /locus_tag="IVM62_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6844155.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(77197..77514)
                     /locus_tag="IVM62_000161"
     CDS             complement(77197..77514)
                     /locus_tag="IVM62_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6844156.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(77540..77860)
                     /locus_tag="IVM62_000162"
     CDS             complement(77540..77860)
                     /locus_tag="IVM62_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6844157.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(78324..79739)
                     /locus_tag="IVM62_000163"
     CDS             complement(78324..79739)
                     /locus_tag="IVM62_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6844158.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(79756..81072)
                     /locus_tag="IVM62_000164"
     CDS             complement(79756..81072)
                     /locus_tag="IVM62_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6844159.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(81222..81920)
                     /locus_tag="IVM62_000165"
     CDS             complement(81222..81920)
                     /locus_tag="IVM62_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP6844160.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(82209..83198)
                     /locus_tag="IVM62_000166"
     CDS             complement(82209..83198)
                     /locus_tag="IVM62_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP6844161.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(83278..84111)
                     /gene="msrA"
                     /locus_tag="IVM62_000167"
     CDS             complement(83278..84111)
                     /gene="msrA"
                     /locus_tag="IVM62_000167"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6844162.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(84286..>84798)
                     /locus_tag="IVM62_000168"
     CDS             complement(84286..>84798)
                     /locus_tag="IVM62_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844163.1"
                     /translation="MTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMD
                     YFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVY
                     AYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLC
                     LFFKIVFHKR"
BASE COUNT        24571 a        17879 c        14476 g        27872 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8341 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     8401 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8461 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8521 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8581 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8641 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8701 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8761 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8821 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     8881 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     8941 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9001 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9061 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9121 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9181 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     9241 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     9301 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     9361 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     9421 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     9481 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     9541 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     9601 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     9661 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     9721 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     9781 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     9841 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
     9901 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
     9961 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    10021 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    10081 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    10141 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    10201 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    10261 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    10321 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    10381 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    10441 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    10501 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    10561 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    10621 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    10681 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    10741 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    10801 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    10861 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    10921 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    10981 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    11041 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    11101 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    11161 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    11221 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    11281 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    11341 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    11401 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    11461 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    11521 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    11581 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    11641 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    11701 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    11761 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    11821 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    11881 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    11941 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    12001 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    12061 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    12121 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    12181 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    12241 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    12301 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    12361 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    12421 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    12481 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    12541 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    12601 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    12661 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    12721 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    12781 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    12841 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    12901 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    12961 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    13021 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    13081 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    13141 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    13201 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    13261 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    13321 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    13381 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    13441 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    13501 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    13561 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    13621 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    13681 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    13741 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    13801 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    13861 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    13921 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    13981 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    14041 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    14101 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    14161 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    14221 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    14281 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    14341 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    14401 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    14461 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    14521 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    14581 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    14641 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    14701 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    14761 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    14821 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    14881 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    14941 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    15001 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    15061 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    15121 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    15181 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    15241 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    15301 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    15361 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    15421 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    15481 acaagaaata atttcaaaaa caacttctcc tgtttttggt ttggttggct tattatcgag
    15541 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    15601 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    15661 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    15721 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    15781 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    15841 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    15901 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    15961 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    16021 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    16081 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    16141 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    16201 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gccactgata
    16261 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    16321 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    16381 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    16441 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    16501 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    16561 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctggcgtt cttttttatt
    16621 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    16681 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    16741 agcaatccac aaagcttggc tttctcctgt ttctccaacc cgcaactgat atttaggacg
    16801 aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa
    16861 tttcttgatt tctccataat gggtcgtggc aatgatcaat gccccttgct catacataga
    16921 ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc tctgttccac tgccaatctc
    16981 atctaaaagt accaacgtat tcctttttat ctttttcaaa atagcagcga tgttttgcat
    17041 atgaccagaa aatgtactca acgcattttc taaattttgg tggtcaccga catcgacgaa
    17101 aatctcatca aagacagcaa gctctgtccc ttcttttgcc ggaacttgca taccgaacat
    17161 cgccatcaaa gtcaataaac cgactgtctt caacacgact gttttgcctc cggcatttgc
    17221 tccggtgata atcaagcctc gatagtcttt accgatttca acatccaatg gtaccgcatg
    17281 ctcaggtaaa aatggatgtc tgccttgctt gataatgata tgttccgatt tattgattct
    17341 aggtgtgctt ccattgattt ctcggctaaa ttttgcccga gcaaagatga tatctaacac
    17401 cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt tctgcgatag caccagtcaa
    17461 tgccgccagg acttgatatt cttcactgat ttcttcagct tttaacaact gatagtgttc
    17521 attcaattta gaaatcacag ttggttcaat aaaaaccgtc gttcttttat ttgattcatc
    17581 gataatcgtt ccatctattt tatttttata gctggctttg ataggaatcg tatagtattc
    17641 gcctttttga acaatcattg cttcttggat catttctttg tttttcggat gacgcaagaa
    17701 tttcagtaat tttgactgga tttctttttc gattagttgt aattgtttgc gtactttacg
    17761 caaattacgt gacgcgtcgt cactgacttt attatttttt attttctggt caatcaactc
    17821 ttctacattt atcaaatcag gaagatgttt gctataagca aacaacaagg gtgtctggta
    17881 ttgattctta tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc
    17941 gatcaaatcg gttggctgca gaatcaaccc ttttttgact tgttctgtca gtgcatcgat
    18001 cctcggcaac cccataaatg gaacatgctg actgctttct aagatcaagc gtgcttctct
    18061 tgtttcttct tgacgatcca agacagtttg taggtttgtc gagaccgtca tatcgctgat
    18121 tcgttttttg ctatagtttc caattgctct tgcttggatt tcctttttga tccgatcaaa
    18181 ttgtaattga ctaatcattt cgttgtccat attctctcct tttcttgaag agatacctgt
    18241 cccaaaataa ataagggcct agagataaat aaaaggatag aacacagctt aaaaaagtca
    18301 tgtcctatcc ttattagaat acaaagatat tgttttcctg acaggtaccg tttcataagc
    18361 acaccaaata aactttgttt attggctatt cgacttactg attagatacc agctacactc
    18421 acaaaacata ccccattctt ctaggtctta tttattttcc acttaactat agcaagacac
    18481 attttggttg tcaaacttta gtatgtttta tagatttaca ccattaatga atagacacct
    18541 accgcgataa ttgctccgat cattggacct gcgattggta cccatgaata tgcccaatct
    18601 gaagttcctt tattagctat tggcaatacc gcatgtgcta atcgaggacc aaagtcccga
    18661 gcagggttga ttgcatatcc tgttggccct cctaatgaaa gaccgatggc taagatcaat
    18721 ccacctacag cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa agctagcaac
    18781 ccaagaacta atacaaatgt accaattaat tctgtgatca catttgctgg ataattccgt
    18841 atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt tgcattccaa
    18901 tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa aagctcctaa aacttgcgca
    18961 atgatgaaag gaacgacaag attccaacta attgcccctg tcatcgccat agctagagag
    19021 actgcaggat tcaaatgagc tgggcccatg aatccagaaa tatagacagc cattgttaca
    19081 gcgagtcccc aaccaaatgc gatgacaatc cagccagaag cctgtgcttt acttttgttt
    19141 aaattcacag ccgcacacac accatctcca agcaaaacta ggatcagtgt cccaataaac
    19201 tctcccatga tttgagtcat atcactgttc atttatttct ccccctttag ttcccgcaga
    19261 tctgattctg caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc
    19321 cattcaaaat atccagcaat tgcatcgatc acaggttgct tgatctcatc aagctgatct
    19381 cgttcaaaca acagatgatt tgtacgacga atgagataat cgcctggggc caaaaccatt
    19441 tcttcttcta aaccataacg taaccgagct gactcagcga gactcaaacc aggataaggt
    19501 gccatttctt ttgctaattc aaagatacgt cgggcattag tcccgtaaaa atcagcgata
    19561 taagtagcat cttcttcctc taaaccggct gctactcctt cttttgctaa ttctgtcact
    19621 gtttcttcta atttagttgg atcgaagttt ccacctgaaa tctgatattt tttagagtcg
    19681 atttctttcg tctctattcc gtattcttct tttaataatt ggcgtatcaa tcgcaaagct
    19741 ccttctgcca tcttacggta atcggtgatt ttcccaccag ataaagtcag caaaccatct
    19801 ggttctcttt ctaaagaact acctctggaa atcgtagaag gtgctttttc atcacggctg
    19861 ttttccaaat ggttcaacac gtcttctact tcagcacgag aaactttgtt ttccttatat
    19921 tcacttactg tatcgacaac tttattgaag ctcttgtctg aaatcgatcc attatctcca
    19981 ccattgtaat cagaaccaga attaccaatc aacagtggac gaagtcctgc ccagcttgct
    20041 tcgatatcat tcaatgtgat atttgcttct ggatagcgat gattgatcac atccaataga
    20101 taatctacat cttcttgtgt gactttcgga tccgtaaaat ctccttggta atccgtatct
    20161 gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa aaaccatccg cccatcttgc
    20221 tttcctgtat cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc tacaactaaa
    20281 tggatccctt tagttggacg cattttagga gagactggac gcgtaaaatt caagtttcgt
    20341 actttatcta cccaaggacc actcgtattg atcactaatt ttgctttgat ttcgatcact
    20401 tcatctgtca gcaggtcacg agctttgacg ccaacgattt gatcgccatc atataaaaag
    20461 ccaaccgctt tcattttact tactagataa gccccatctt cggcagcctt tttgatatta
    20521 tcaatcacta aacgggcatc attgttgcgg aaatctagat agacacccgc gccttttagc
    20581 ccttcttttt tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag cgtataattc
    20641 tcatatttgg ttcctgtcac atttgccagt ttgtcataaa ggtccattgc tactttgacc
    20701 gagaacatat tgaaggttgt tgctccttcg ccctcataga ttggcaaaag cattggatct
    20761 ggttttggga tgtgtggggc aatactttgt acgactgcac gttcaccaac tgtgtcagct
    20821 actacttcca catcaaatgt cttcagataa cgaataccgc catgaacaag tttggtcgag
    20881 cgagaggaag tcccttctgc aaaatcctgc atttcgatca atcctgtttt gattcctgat
    20941 gctgctgact ggactgccac accggcacca gtgattccgc caccgatgat caacagatcc
    21001 aattcttctt gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg
    21061 atttcctcct tattcaaatg cttgggtagc ttttactgct tttttccaac cgttgtaaag
    21121 tttttctcgg cgctcttcct ccatttgaac ttcaaatagc ttgccagctt cataaaattc
    21181 gcggatctcg tctgtatctt tccaataacc gacagctaat cctgctaaaa atgctgcccc
    21241 tagcgccgtt gtttccaagt tttctgctcg ttggatcggt acattcaaga tatctgtttg
    21301 gaattgcatc agatattcat tgtttgctgc accgccatct actttcaata caggaatctt
    21361 gattccagtg tcatcttgca tcgtatcgat gatatctctc acttgataag caatcgactg
    21421 taaagtagct ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa acatagcccc
    21481 tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg aatgcaggaa caacataaac
    21541 ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat
    21601 ttgaagtcca tctctcagcc attgaatcga agaaccagcc acaaaaatac ttccttctaa
    21661 agcatagtaa acctttccat ttatgccgta accaattgtc gtcaataaat tatttttgga
    21721 taattgcggt ttttcaccgg tattcatgac aataaatgaa cctgtaccat aagtattctt
    21781 gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt ctcctgccat
    21841 ccctgaaatt ggtgcttcgc ttccataaaa atgataacct tgcgtataac cataaacttc
    21901 tgagttagaa gtgacttttg gtaacatcac tcgaggaata tttagtaaat ctaaaatctc
    21961 ctgatcccaa tctagctgat ggatattgaa caacatcgtc cgactggcat tcgagtaatc
    22021 agtaacatgt gcttgtccat cggtcagttt ccatactaac caagaatcga tcgttccaaa
    22081 catcaattcg cctttttcag ctcgttcttg cgcgccttct acatgatcaa gaatccagcg
    22141 tatcttcgtt gctgagaaat aggcatcgat cactaaccct gttttttcgt ggaaaaattc
    22201 ttgatagcct tcatttttta agttatctgc aattccagct gactgtctgg attgccaaac
    22261 aactgcatga tagattggac gtcctgtatc tttttcccat attactgttg tttcacgctg
    22321 gttcgttatg ccaatcccag caatctgatt tggtttgatc cctgattcga tgaacgctcc
    22381 ggcaataact gactgtacag aattccagat ttcatttgca tcatgttcca cccaactttc
    22441 ttgcgggaaa tattgcgtga actctttttg agaactgccg atgtgtcttc ctttcttgtc
    22501 aaagataatt gctcgtgagc tagttgtacc ttggtcaatc gccatgatat acgttgattc
    22561 tgtcattgtg attccctcca ttatatgcaa acgctttctt taactactct tatcatacta
    22621 ctgttttaaa aaaagaaact gtcaaaattt tattcttttt ttcttttttt gacagttttt
    22681 tattatgaag ctatttttta atcaattctt ttcttttttc agcgtctcta ttcttctttt
    22741 caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga taatattcac actgtttttt
    22801 gtttctctgg caaaatgtga tcaccaaatc atattcttta ccttcttcat aacttccaat
    22861 ctcaataccg ccgattcccc tcagttctct gatcagatat tgttgaaaag caatacgata
    22921 aatcggcaga ctttctaaat cgattccaat cactactggt tttgcaatat aaaaatcgat
    22981 catcaaaaga acattggcat actggatcat caggtaacta agctcactat cttcagctct
    23041 tttcctcggc aattgctgca ccgctgtttc ctgtaatttc gccagtaact gtgttaagga
    23101 atgcccgagc attttctgct gctgttccaa aagccgatca cgctcataga cttcgatctt
    23161 tccaacaaag aaaaaccatt cattgttgat ctgggaaatt tggtagccta ctgccttttc
    23221 ttctttgatt ttcaaacgac gaggacgata atgccataac atcgtttccc gaatgtaagt
    23281 atctaaaaca gcggttggta actttttgct ccgtgtcaaa tcatactgat aaaatatttc
    23341 ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt
    23401 cagttctgct gctgttcgac taagatgcat cacgatgatt ggatcgattg cttgataaag
    23461 ccggtccgac tgatagagta atttcttttc tttcaaagtt gcgtacgtac ttttttcatt
    23521 caatagtctt tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt
    23581 aaacgttgtt tctaatacac ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt
    23641 ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg aatagttcat aataaaaata
    23701 tcgtatctgt agctcttctc cgtataattg accgtttttg atttgtaatc caaattcctc
    23761 taagagctgg ttcaattctt tgatctttcg aaaaacagaa gactcactga tattaaacgc
    23821 tgtcgctaat cgaacgatgg atacttgctg atgttctaat aaatagacga gcatcttaaa
    23881 cttcaagctt tcctgcaata aaaacaatag aattttttcc aaacttacat cttcgtccaa
    23941 cacaagcagc gcctgatact catttcgctg tacttctact tttttttcca tcattcggcc
    24001 aatcaattca atatcttcca aatattgatc aaacgaggct tttgacagtt ggacatgctc
    24061 tctcaaacca ttgatagagg ctgttccccc ttgcagaaca aggtttcgca aaatcaggat
    24121 ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc ataggcagta ttcgccacct
    24181 ttgaccacac catatctggc acacggtatt ctggctggaa aaatagctca tgttcttcta
    24241 actgttcaaa cgtccatcct tcttctactg caattgtcag ttggtcaaga aggtggaaca
    24301 tctctttttt gctttggagc tgtgctccaa gaatcttttt ttcagaatca aatagaaatt
    24361 tgagtttaac tttgctttga tttattgctg taagcttgta gtctacgtat cctgtttcaa
    24421 ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc
    24481 caaaccactg attgccgact ggacgatagg ttccctgatc ctttgtctgt gagccactaa
    24541 ttgtcttgct gacatttcgt gctgtacgga cagcgttatt tactagagaa acatacattt
    24601 tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa acgtcttttt
    24661 gagacgtttg caagtagtca tctacccaga tcgttccatc atcattcaat gttaattgtt
    24721 cttcccacat ctgattgtcg gggcgagaat gattagccag caaaacaaga tcatagttct
    24781 cctttctttt ttccgtctct acaatagcgg tttcattttc ggtcaaagac acaacttgtt
    24841 cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt tactaacggt tctgtcattt
    24901 caggatctag ataacgaaaa agaattgact ttcggctttc gtaaagatgg atttgttttt
    24961 ttcttgatgc caacccttca gctatttcga ttcctgcttg tcctgcacca ataattgcaa
    25021 ttttctttgc attcatgatc ttttgctgaa gagccgttac gttttgtatc tttttaactg
    25081 ggtgaataag cgttgattct gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta
    25141 gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa aacgactttt ttttctttca
    25201 atcctattac tgtcttttct gtgtaaacat ggattcgata gcggtcagtc agttcttctt
    25261 tcgtgatcca agaaagttgt ttttcactgg taacctttcc ttttaatatc aaattgaacc
    25321 cgctgggaat ccatcctaac ttcgattgct tttcaatcaa atatatttca cttgttggca
    25381 tcaattggcg aagataaata gccgcatgaa tccctgcaaa cgacccgccg acaattacaa
    25441 tgttcatctg cattcctctt tcttataagg tgttctactg ctaacttctc aatcagaaaa
    25501 cacagtattc ataactcgct ttcaacctta tgatacatag cgatgcagca gaatcaaccc
    25561 tctccatcat accgtgttct aggtctgctt ctcaataatg tactagtgga cctaattgaa
    25621 gagtatcatt tctgaagaga tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc
    25681 cttttacgta cctctgtcca aataggaaac agaaaagaac gaaaagacga gttttagcgt
    25741 gtgtaatgta atagttagaa tagacacatc atttattttt aattctttaa tgcttcaaca
    25801 actgcttcgc ctaattgatg agcagattga ggattttgcc ctgtaagaag ccgcccatca
    25861 atttgaacca cttctgaaaa agctgctcca gactgatagt ttgctccttt ttctttcaaa
    25921 gcatcttctg ctaagaatgg tacttcattc gttgtaccat ttgcttcctc ttcttcatta
    25981 ctgaacccag tcactgtacg tccagctaaa atagattttc cagactcatc tgtaactggc
    26041 aacaatccta caacaccgtg acaaacagct gagaccagcc cttcatttcg atagatttgt
    26101 gaagcgactt tcccaagatc tattgcttct ggaaaatccc acattgttcc gtggccacca
    26161 gcataataaa ccgctgcata atcatttgca tccacatcat caggacgtaa ggtatttccc
    26221 aaggcccgat ttctgaaatc ttcatcttca taataggccc aatcgatttc atccatattc
    26281 aaaacacttt gcggttctaa aggtacatat ccgcctttag gactgacaaa atctgcatcg
    26341 atcccatttt ttactaatac atcataaaaa tgagtcagtt cacttaacca caaacctgta
    26401 ggacgctcga tcttttcgta cttttctaca tttgttaata cgatcaatac tttcattttc
    26461 ccatcctctt tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa ttatataatg
    26521 ggcttggcaa ataaagctct ttatatgctt cttcaaaagg tttcgttaca aattcatcct
    26581 cacggttttc caataatttc tctgcccagt caggatcgac gatcatttgc tgtccaattg
    26641 ctgcaagttc tgcatatttc aatacctgct ctacatcttc gcgattgcgg atcccaccga
    26701 caccaactaa agggatcttt ccttgaatcg tgttatggac cacttcaaga atcgatttat
    26761 cgttgtattt atcagaacga gctattcgat catacatatt taatgaaaca tgcagataat
    26821 ctagttttga ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg
    26881 gtgtttcata ttcttctggt gagaaacgat accctacgat gaatggtttt ttagcatatt
    26941 gttcaaccgc tgagaaaacc gttgttaata attcttcaat aaaacgataa cgattttcca
    27001 agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat tgttggatca
    27061 aatacgtatt tgctccatgc aactcgattc catcaaatcc tgattcaatc gctctacgtg
    27121 tggctttgcc aaatgcttgg attgtttcaa cgatttcttc ttcactcatt gctcgtggtg
    27181 tttccgcatc aggacgtttt gctgcaactg cacttgcaga tacaggctgt tctccagata
    27241 aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt
    27301 ctccttggat tgctttcgct agctcactta atcgaggaat catttcatca tgagcgatgc
    27361 tgagttcacc tggccagcct tttccgatat cttggacatt tgcagcacca gtaataacag
    27421 caccaagacc tttagaacgt tgtttataat aagtgatctc atcttcggtg atcactccat
    27481 tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa tcggttcttc aattgaactc
    27541 ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt cattcctccc ataattcgct
    27601 ctgattaacc tatcgagaat catctatcat taaaccactc gattagttgt acatgcaact
    27661 aatttgctta aaaaatttta ttttttttgt aacttatctg ttgcatcatt cattgtctgg
    27721 gctaattctt tacttgcttc atttcccaaa actgctttgt atgactgatg aagttcttcc
    27781 cagctaccat cgacttcctt agcaaattgg cgtccttgtt ctgtaagata aactaatgac
    27841 aagcgacctt cgctaactgt tttgacgtat cctttatatt ctaatttttc aacaaaacga
    27901 gcaatcgttg atggggcgga atagagcatt tgtgcaatat ctttttgtgg tattccatct
    27961 ttttctttta atatcaataa aataaaacca taagttggtg ccagacctgt ccttctaaac
    28021 gcttcatcag ctaatttatt caacatgcga tctagctttt tcacgctaaa gaaaagacat
    28081 tcatctaacg ctttttcaat tggcttttcc atttcatccc ctccattggc gtacatatca
    28141 tagtatgatt tttatttttt gtcaaaaaac acggaataat gtccgcctcc tctgtctaga
    28201 tttgaaaaaa agaagtagcc atcttcaaag aatttcggct tgttttcgac aaaactcctt
    28261 ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa
    28321 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg aaaaactggc
    28381 ttttgaacac cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa
    28441 cagccaaaca aaaatagttc ctcatatttt gtcgtttttt gagctaatgc cgatggtcag
    28501 ctgattgtac accgtttatt gagaggtcgt gagcctgccg ttgaactccg agtcacaagg
    28561 agcaatttca aaaaacagct tctcatgttt tatgactatg gtgacttgtg atcgaggagt
    28621 tggcaggaga atactgttta ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc
    28681 ccttatttcc ttttattata ggatttcgcc atttgattgg atgacttgtc tgtaccaatc
    28741 aaatgatttt ttctttaacc gtttctttgt gccattccct tcgtcatctt gatcaacata
    28801 gatatatcca taacgttttg acatttgtga agtagaagcg ctgacgatat caatgcagcc
    28861 ccacgacgta taaccaaata aatctactcc atctttgata gcttccttca tctgctcaat
    28921 atgctgtttc aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt cttctacttt
    28981 gtctatagct ccgagtccgt tttcagctat gaacagtggt ttttgatacc tatcatataa
    29041 ttgattcaaa gcaattctca aacccttagg atcaatctgc cagccccatt cacttgatgg
    29101 aagatacgga tttttaattc ctccagttat taagtttcct tggacccctt cccaattttc
    29161 tggatgagca ctgactgcac tagacatgta gtagctaaaa gaaacaaaat caacagggta
    29221 ggctgctaag atttcttcat cacctacttc tatttttaag ttgacacctt tttcttcaaa
    29281 cattcgttgc gtatacgctg gataatatcc tcttgcttga acatcactaa agaacaaaga
    29341 agttctttgc tgctcaattg ttttttgaat atcatccgga tcacaagaat acggaacaag
    29401 aagctgatag ctgatcatac aaccgacttg tgaacctttg ataatttcat gacaagcttt
    29461 tgtagctaaa gcacttgcta taaactgatg gtgtgcacct tggtatttcg cttgttccaa
    29521 atggggatgg ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt ttacacagtt
    29581 gatctcattg aaagtcatcc aatattttac tttattttta tagcgtttga atactgtttc
    29641 agcgaaacga acgtaaaact gaataagctt tcgattaggc catccaccat attctgtaac
    29701 caaatataac ggcatttcat aatgggaaag agaaacgact ggctcaatcc catattttag
    29761 acattcgtca aacacttgat cataaaaaaa taatcccgct tcattgggtg tttcttcatc
    29821 accatgagga aaaattcttg tccatgcgat agacatccga tagcatttaa aacccatttc
    29881 agcaaataaa cggatgtctt ctttaaagtg atgatagaaa tcatttccgt gtcttttagg
    29941 ataataatac ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg tcatcatatg
    30001 ccattgatct gtccattttt ctttaggtaa atcgctgtcg aatgtataca catcagacac
    30061 agataatcct ttttcatcta catcccatgc accttcacat tgattggccg ctgtcgctcc
    30121 gccccataaa aatccttctg gaaatttttt catatcgatc cccctaaact tgctagtttt
    30181 cttgtttgat tatttttctt atgactagta atgatatttt tgaatctact actatttgta
    30241 acaataacca ttgtggtcgg atcatatccc tctttgatta attcttgcaa atctacttcc
    30301 atcaataaat cgcctttacg tacttttttt ccatcagata ctgcactttg aaagtatttg
    30361 ccatttaaat tcactgtatc aataccgata tggatcagta tctctgctcc ttcttcactg
    30421 actaccccaa ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt tacaggactg
    30481 tagatttttc cgtccgttgg aatgatcgca aacccttctc ctaaagatcc actagcaaat
    30541 gttggatcgt ttacttcaga taaatcaatt atttcacctg ctgcaggagt acttaacact
    30601 atatcctttg aggattcttc ttcgatttca tccttgtagc cgaaaagaaa agttagcata
    30661 caggaactga tcatcgctaa gaaataacta gcaactagcc aaacaaatcc ttcaccaata
    30721 taaatcggga aagacaaaat acttcttgta gcaactgcca aagcaccggc tcctgcggca
    30781 ccagctactc caccaaatat agctccacat atacaagcaa tgtaaaatgg tcgtttatat
    30841 ttaagagtca caccataaac agcaggttca gtgattccaa ataatgctgt gataaatgca
    30901 gataaagata aactttttac tttcttattt ttcgttttca aaaatacccc aagtactgct
    30961 cctgcttgcg cagctacggc agtcatacat gctggaccta gcgtatcacg accgtagaaa
    31021 gaaagattgt tggtcattac tggtacaagt ccccaatgta acccaaaaat cactaacact
    31081 tgccaaaatc cgccgataac agcaccagat aaaacagggc taaagttata aacagctaaa
    31141 tatccagctg caatcagctg acttgcataa tctgcaattg gacctacaac taataaagta
    31201 attgggacca tcacgatgat acaaatgaat ggtgtcaata aatttcgaat actttcgtgc
    31261 aagcttttgc gaatcttagg ctcaatataa gaaagaaccc atacagctaa aattgctgga
    31321 ataaccgtac ttgtatacct tgctagaatc acgggcatcc ctagaaatcg caaggttatg
    31381 ctatcactat atgcactgac aatcgtagga taaacgagag ctgctgcaat aaccatagcg
    31441 atgaaacgat ctgtattgaa cttttttgca gcagtatatg ccaaaaatat aggcaagaaa
    31501 tagaaaaatg catcagctgc tgcattcaaa atgatataag ttcctgattc agtagttaat
    31561 aaattcaaac tcgtacataa ggcgagtact cctttgatca aaccacttcc tgccaagacg
    31621 cctaaagtcg gggcaaaaac agcggcgatc atgtctataa aagagtttaa aatgcctttt
    31681 ttctcagttc ctctctttcc actagatgca tcatcttcta gtttcatttg tttggataat
    31741 acgtcataca cttcatttac tgcatttcct actacgactt gaaacattcc tccagcttcg
    31801 actacagtga tcacaccttc tagttgctct accttctcct tatctgcctt cttattatct
    31861 tttagggtaa agcgtaacct tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct
    31921 ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt ccatttttac ttcccccttg
    31981 ttaattaatg ccgtagcaat ccttcttaca agataagtat agttcttaga aaacggtttc
    32041 tccattcaat tagtacccct tataatagcg aacgaaactg tacaatagtg tgtatggttt
    32101 acttagccga gcaaatcact cactgcttga aagaaaacaa gtatatcgtt tctcttatag
    32161 tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct gatgtataag tggtatctca
    32221 agctgatact ctgtgatttt tgtttctact tggcttttga taagacagca caacaaagtc
    32281 tcggcattta acggaagcat aactaggtca gaactaggag agattagttg ctgaatggaa
    32341 tctatctttt tatggatttt tatttgattt tcttctttgt agtaatgatc aagcagtttt
    32401 gttttctttt tttcttcaac tatggtcagc ttttcacgca aaatctgata aatctcccaa
    32461 gattcaaact catctagaac actaggtaga taaatcaaat catcccactc aaaaataagc
    32521 tcttgctcag tagtcaaaat tgcatcatat tccgttattt tgtttgattg atttttgaaa
    32581 agatacactg gttctatccg gcattctttg atccacatgt tcatactttg ttgaatctgt
    32641 ttttttagtg tattcaaaat actaattggc tggttactaa caatcagtat ctccccttgt
    32701 tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa
    32761 ttgggacgta attgaaaatg tattgggaag aaacgccgaa ttaaataaat ttctaaagga
    32821 taacgggaga tgatcttttg tacaaaatga ttcatggtat tatgtccggc atccatcctc
    32881 gttttcattt gtttgatatg ggttagcaaa aattctgttt catggaacaa taacgaaaca
    32941 ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt ctctatttct
    33001 ttgttttttt catcattaat catcaaataa tttaactcta atagtcgatt ttcaattgct
    33061 tgtttttctt gattggaaaa agaatagggt agttctgatg ataacttttt tgtgagggtc
    33121 tctaccatcg gaataaataa tgtatacctg tttgtttttt ctaaaagttc ttctcttcga
    33181 agaatggtca aaacgagata ccgagaaaat cgtatgaagt cctctcctga aacaatataa
    33241 ttatatgata taagaacctc ttttaatgtc ttttggacag tcggcatgat attggaaaaa
    33301 attgtactga tttgttgagg caaccttgcg tgttggatca ccgtttctgt tccaacaaga
    33361 gataagaaaa ttctttttga attttcttca gcaatgattt ttagtccttt tgtcgacgaa
    33421 acttccagtt ctaatttagg attgcgctcg atccaacgca aaatcgtttt tatttgtaat
    33481 gatacggctg tttttgaaag aaagaaaatg tctgctatct tttgcatact gacataccct
    33541 ttttgaaaca ataagtacgt cacaatagtt gaagctcgga aatccatgct ggaatagatt
    33601 ttatgtttcg tcacctcttg tatgatttgc tgtttagttt cagattcaat tgattctaaa
    33661 aaataacctg aacgtttatt tgactggata atgattttct tctcgtagat agcaagcata
    33721 tttttgattt cagtttggat cgttttcttg ttggtaccta aagcttcagc taattcttcg
    33781 cttgttatcc aatgattctt atttcttaat actacaagaa tttggagttg tcgtttattt
    33841 aagtccattt gcttacacct cctctttgtt ataaacatac aggaaaaacc tctcaaatga
    33901 caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa gatctatatt caaggttttt
    33961 accattagac tggataattt gtttataata ttcaaaacta tcttttttca atctcctaaa
    34021 gcttccgctc ccatcatttt gtctatccac ataaataaag ccatatcgct tcctcatttc
    34081 ccctgttcct gccgatacga tatcgattgg tccccaccat aagtaaccaa tgatttggca
    34141 accatctttt attgcctcag caatttcctg aagatgtatt tttagatact cacaacgttt
    34201 tggatcgtgg attcgattca attcatctgg tgcttcatct agtccaatgc cattttcaac
    34261 tatgaataat ggtaattcat aacggtctgt caattcattc atgacataac gaaaccctag
    34321 aggatcaatt ggccaactcc aaggttcggg agatttttcc ttcaaaaagg gattatcgaa
    34381 accatttgct ccgccagtat ctacgcgcat ttctgcttct tttttataga cagcgctacg
    34441 ataataacta aatgcaatat aatctgccgt gttagcttgc aagcactgtt cgtctccatc
    34501 taacattact ggtgcacagt cttcttcctt ccatgtcctg taaatataag ctggataatg
    34561 ccctcggcac atgacatcgg agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa
    34621 tacattctct ggatcacagt catatgcata agtcgctaat gcagaaaagc tacacataat
    34681 acccatctta gcatttggat cgattttctt taataattga gtagcacggg cgttcgctag
    34741 aaacatatga tgacaggctt gataaatcac tttttttgtt agtgtagcaa tgggttcaag
    34801 tttatctcta gcagggaatg taaccaatcc agcagctgca taaggcatac gaaaagcatt
    34861 attgatctca ttaaaggtca tccaatattt cactttccct tggtagcgtt caaagacggt
    34921 ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt tgccaccccc catactctcc
    34981 taccaatgct aagggggttt cataatgact aagagtaatt actggctcga tatcatatcg
    35041 atttaatgta ttgattagtc tgtcataaaa tacgagacca gcctcatttg gcttctgttc
    35101 gtctcctctt ggaaatattc gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa
    35161 ctccttcagt aaacgaatat cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga
    35221 aagataatgg agtgattcgt ctaaagcaat ctcccaaatc tttttttctt cattccactg
    35281 aatactagga tgctggttca tgatcccgat aagcgtatct gtcacattag gtaatttgcc
    35341 gtcttccaac catgcacctt cacattgatt agcagctact gctccgcccc ataaaaaatt
    35401 ttctggaaat atttcctgat ccataactat ctctcctcgc tatcaacaat ttcttacttg
    35461 aagtatagct cgattcatca aaacaagata ttctaactat ttcggcagaa gagaagaaaa
    35521 aaattgaatg ctactcttca tttttaatat ttatatataa caaatacata agacgatccc
    35581 cgaaagaaaa cactcgttaa ccatctaagc aaacgattta ttttacttcc aacacaaatg
    35641 cttcataagg tcgtaaatca actttgccgt ctggtaactg tttcagagga taatttgaca
    35701 caataaccct taaagcttca cgtggaacag tgaacgtctg cttttcactg gacatattac
    35761 aaataacgag ccattgctgg tcttcatata tgcgagaata ggcaaagaca tgctcaggcg
    35821 tcttttccac taattcatag tctccccaga cgatgatgtc atgttttcta cgcaaatcga
    35881 tcaatttctt gtacatataa aaaacagaat cttgatctgc taacgcttct tctgcattga
    35941 tttctttata atttggattg actcgaagcc atggaattcc agtagtaaac ccagcatgtt
    36001 cacgggcgtt ccattgcata ggcgtcctag cattgtctct tcctttcgca ttgattgatt
    36061 ccaagatttc ctcttttgta aatccagaag aaatcctttc gttatacata ttgattgtct
    36121 caatatcttt tgcttccgcg atatcatcga ttgggcaatt cgtcatgcct aactcttctc
    36181 cttgatagat ataaggggtg cctttcatca tatggagcaa gatagcaaac atcttggcac
    36241 tttcttttcg atacacatcg tcatttcccc aacgagaaac aattcgcgga agatcgtgat
    36301 tattccaaaa taaactattc catccttcat cacctaaaga tgtttgccat ttggataata
    36361 cttttttcag ttcagagata ctcaagggtt tcaaatccca tttgtctttg ccttcttgct
    36421 gatctaatcc tatgtgttcg aattggaaga ccatggataa ttcatttctt ttaggattgg
    36481 aataaagttt agcgatttcg gtagttgctc cccatgtttc gccaacagtc atcagatttt
    36541 tgtcaccaaa agttgcttga ttcatttcct gaagatactc atgcagttta ggtccattac
    36601 ttgtgatttc tttatccggt tctttgccaa tcatatcgat cacgtccata cgaaagcccc
    36661 caataccttt atcgatccag aaattcatca tttgataaat ctcttggcga acctgtttat
    36721 ttttccaatt caaatcaggt tgacgcttgc tatacaagtg cagaaaatat tgccccgttt
    36781 tttcatccaa ctgccaagca gaaccactga atattgagcg caatctatta ggctcttttc
    36841 cattgaccgg atcacgccaa atataatagt cccgataagg attttcttta tttttacggg
    36901 cctcaatgaa ccacggatgt tcatcagaag tatgattcac caccagatcc atgatgatac
    36961 ggatatttct tttctttgct tcttcaatca gttcttccat ttcttccatc gtaccgaatt
    37021 ctgccatgat tgcttgataa ttactgatat catacccatt gtcatcattc ggagactcgt
    37081 atactggaga aagccaaatt gcatcaatac ctaaatacgc cagataatcc aacctggaaa
    37141 taatcccccg caaatctcca ataccatctt gatttgaatc ttgaaaactt cttggataga
    37201 tttgataaac aactgaatgg tgccaccatt tttgttccat gattttcctc ctataaatgg
    37261 atgtttctat tttttgctta atcatttatt ttagtctctc tcactaaaaa ttgtaacgct
    37321 ttcaaatagc tattgcacct tgttatcggt aacagaataa aacaaaaaaa cagctgagga
    37381 aattccctca actgtttttt gaaaggattg atttatgcag ggaagaatgc tgatttcaca
    37441 acttctttat aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaacggcaaa
    37501 atatgaggaa ctattttttc gtttttttct ctaatactca ggccagaaca ctgatttcac
    37561 aacctcttta taaacggtgt tcactcagct gtccctcggt attagctcaa aaaacggcaa
    37621 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    37681 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    37741 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    37801 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    37861 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    37921 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    37981 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    38041 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    38101 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    38161 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    38221 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    38281 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    38341 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    38401 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    38461 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    38521 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    38581 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    38641 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    38701 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    38761 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    38821 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    38881 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    38941 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    39001 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    39061 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    39121 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    39181 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    39241 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    39301 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    39361 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    39421 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    39481 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    39541 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    39601 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    39661 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    39721 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    39781 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    39841 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    39901 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    39961 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaaa
    40021 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    40081 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    40141 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    40201 accgctgctt gtgtttcttc cgtcagcatt gttctttggg cttcacgcca gttgagacag
    40261 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    40321 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    40381 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    40441 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    40501 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    40561 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    40621 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    40681 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    40741 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    40801 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    40861 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    40921 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    40981 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    41041 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    41101 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    41161 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    41221 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    41281 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    41341 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    41401 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    41461 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    41521 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    41581 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    41641 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    41701 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    41761 tttcagccat ttcatcccac ctttattttg tttgtaagac tagtttacct ctttttgttg
    41821 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    41881 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    41941 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    42001 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    42061 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    42121 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    42181 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    42241 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    42301 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    42361 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    42421 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    42481 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    42541 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    42601 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    42661 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    42721 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    42781 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    42841 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    42901 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    42961 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    43021 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    43081 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    43141 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    43201 cgatcacgtt tgtaccgacg ttgacataac acttcaaagt ctgctgttag gcgaatcgtg
    43261 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    43321 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    43381 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    43441 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    43501 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    43561 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    43621 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    43681 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    43741 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    43801 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    43861 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    43921 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    43981 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    44041 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    44101 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    44161 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    44221 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    44281 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    44341 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    44401 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    44461 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    44521 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    44581 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    44641 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    44701 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    44761 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    44821 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    44881 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    44941 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgatgaat cccatcgaat
    45001 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    45061 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    45121 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    45181 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    45241 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    45301 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    45361 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    45421 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    45481 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    45541 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    45601 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    45661 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    45721 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    45781 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    45841 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    45901 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    45961 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    46021 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    46081 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    46141 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct
    46201 cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc
    46261 aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc
    46321 gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga
    46381 aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac
    46441 atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa
    46501 aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga
    46561 aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc
    46621 ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac
    46681 tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg
    46741 ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg
    46801 ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat
    46861 catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag
    46921 ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa
    46981 aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa
    47041 taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac
    47101 atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag
    47161 ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg
    47221 atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt
    47281 gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat
    47341 ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt
    47401 acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt
    47461 cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc
    47521 ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat
    47581 gttttctttc aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc
    47641 catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt
    47701 ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat
    47761 tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc
    47821 tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa
    47881 gctactgtct tttatttctg tatttttcca attaatggca gagataaatt catgtttcct
    47941 gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc
    48001 ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa
    48061 ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc
    48121 ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga
    48181 acctttataa accagtcctt gctttccata actgacgatc tctatcccat aactgaccag
    48241 tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc
    48301 ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa
    48361 aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg
    48421 agcttgcttt tccttttcca gcttcaacaa aaaaactttg ttgcgaattg tacggatctc
    48481 cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg
    48541 ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat
    48601 gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca
    48661 agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga
    48721 aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga
    48781 catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc
    48841 tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg
    48901 gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca
    48961 gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca
    49021 aaatcgtgcc ttattgccga taggctatct ctggcacact gtttattcag aaaaaggagc
    49081 tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc
    49141 ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc
    49201 tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg
    49261 actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt
    49321 cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct
    49381 ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg
    49441 gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt
    49501 tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct
    49561 ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact
    49621 gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga
    49681 ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat
    49741 gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga
    49801 ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa
    49861 atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa
    49921 ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt
    49981 tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag
    50041 tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc
    50101 caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta
    50161 aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac
    50221 tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt
    50281 gtttctgtac atatgaatca ttatctagct ccttagctgc ttggattgca agcctgatat
    50341 gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc
    50401 tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc
    50461 tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa
    50521 tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg
    50581 tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact
    50641 cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg
    50701 tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg
    50761 catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca
    50821 acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc
    50881 cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta
    50941 accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc
    51001 ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata
    51061 cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg
    51121 gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac
    51181 tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat
    51241 gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat
    51301 aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat
    51361 aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag
    51421 tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca
    51481 atactggcca tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg
    51541 gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac
    51601 ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg
    51661 ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa
    51721 acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa
    51781 caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa
    51841 aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca
    51901 taacagccgt tacagctgat actttctgga agtcacttac gttgattttg atttttttgt
    51961 tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg
    52021 tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga
    52081 aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga
    52141 ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc
    52201 actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat
    52261 tccccattta catcctgata atatgtttct agcagcatga aattgctgat aaacaggata
    52321 atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac
    52381 ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg
    52441 taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat
    52501 cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa
    52561 aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc
    52621 acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt
    52681 tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat
    52741 gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata
    52801 gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc
    52861 agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat
    52921 cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg
    52981 tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc
    53041 ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa
    53101 ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt
    53161 cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc
    53221 ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac
    53281 gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact
    53341 aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact
    53401 tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg
    53461 gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc
    53521 aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat
    53581 tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat
    53641 ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga
    53701 atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct
    53761 cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa
    53821 tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat
    53881 tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc
    53941 acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac
    54001 atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa
    54061 ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac
    54121 gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg
    54181 atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc
    54241 gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta
    54301 ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat
    54361 aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg
    54421 tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc
    54481 ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat
    54541 aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt
    54601 attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta
    54661 tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc
    54721 atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc
    54781 ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt
    54841 cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt
    54901 ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct
    54961 ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa
    55021 ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa
    55081 tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga
    55141 atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg
    55201 tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa
    55261 catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc
    55321 gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc
    55381 gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg
    55441 atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata
    55501 agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg
    55561 tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc
    55621 tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt
    55681 cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc
    55741 gttgacttga acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt
    55801 ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac
    55861 taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg
    55921 gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc
    55981 accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc
    56041 tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg catctacttt
    56101 ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt
    56161 gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt
    56221 gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc
    56281 ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc
    56341 gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt
    56401 tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc
    56461 taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa
    56521 caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata
    56581 caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt
    56641 atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc
    56701 ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt
    56761 atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt
    56821 tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc
    56881 gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc
    56941 aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc
    57001 aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag
    57061 taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata
    57121 ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa
    57181 cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc
    57241 acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa
    57301 gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc
    57361 agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta
    57421 tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt
    57481 tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat
    57541 atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg
    57601 agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact
    57661 tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt
    57721 taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga
    57781 ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg
    57841 ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa
    57901 agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga
    57961 cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag
    58021 ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc
    58081 gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag
    58141 atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa
    58201 aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca
    58261 gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta
    58321 aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta
    58381 gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt
    58441 tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa
    58501 tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata
    58561 ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg
    58621 accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta
    58681 aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc
    58741 gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag
    58801 aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc
    58861 aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt
    58921 attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag ttcatgatga
    58981 aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa
    59041 cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt
    59101 cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac
    59161 aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg
    59221 aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca
    59281 tcatcagcga tcaagcagga attagtttgc aaaaccagca aaatttatta acaaatgacc
    59341 tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta
    59401 caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga
    59461 aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga
    59521 cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat
    59581 tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc
    59641 attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga
    59701 ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag
    59761 attcttctat ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat
    59821 tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct
    59881 ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc
    59941 attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc
    60001 ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc
    60061 atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat
    60121 atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa
    60181 aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac
    60241 aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac
    60301 cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct
    60361 tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga
    60421 tttccaattt ccctctagat tctctagata tccctattgt atacatttct tctattccaa
    60481 cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc
    60541 tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct
    60601 ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt
    60661 gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc
    60721 ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct
    60781 tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag
    60841 acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat
    60901 aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca
    60961 gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac
    61021 tcgaacccta gttcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt
    61081 actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga
    61141 gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg
    61201 ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct
    61261 gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc
    61321 aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc
    61381 aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt
    61441 ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact
    61501 ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc
    61561 cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca
    61621 ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc
    61681 tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga
    61741 ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga
    61801 gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc
    61861 tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt
    61921 tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg
    61981 ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata
    62041 atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt
    62101 gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa
    62161 ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc
    62221 atggtcaaaa cagctggcaa tcctttgggt accattgaca ccaccaaagc aatcgtagcc
    62281 gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg
    62341 ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga
    62401 ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg
    62461 tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa
    62521 gccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca
    62581 gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt
    62641 aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc
    62701 agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc
    62761 atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg
    62821 agtccattga cgatcactac gaataaaata atacttcctt caatatattc actagaagca
    62881 aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct
    62941 atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc
    63001 aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat
    63061 acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg
    63121 ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa
    63181 ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata
    63241 caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt
    63301 cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc
    63361 tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag
    63421 accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac
    63481 tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa
    63541 taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt
    63601 cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca
    63661 aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt
    63721 gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata
    63781 aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg
    63841 cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa
    63901 ttccagcaaa actgaataaa accataggaa atgagactac cattccagca aagccaaacg
    63961 gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat
    64021 ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga
    64081 ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta
    64141 agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga
    64201 tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag
    64261 tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag
    64321 agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag
    64381 ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg
    64441 ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc
    64501 gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca
    64561 agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt
    64621 actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct
    64681 ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa
    64741 actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca
    64801 tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata
    64861 acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa
    64921 aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca
    64981 aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct
    65041 gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag
    65101 acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc
    65161 gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca
    65221 actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga
    65281 atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca
    65341 gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt
    65401 attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc
    65461 cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca
    65521 ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt gatgatccaa
    65581 taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg
    65641 ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt
    65701 tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc
    65761 caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag
    65821 attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt
    65881 gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag
    65941 gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat
    66001 agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca
    66061 tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg
    66121 gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg
    66181 gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc
    66241 tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc
    66301 gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc
    66361 atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt
    66421 ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc
    66481 ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat
    66541 ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca
    66601 gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac
    66661 ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg
    66721 agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca atggcaatac
    66781 acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata
    66841 agctcctaac atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga
    66901 aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata
    66961 gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc
    67021 atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc
    67081 agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata
    67141 gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc
    67201 tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac
    67261 agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa
    67321 agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc
    67381 gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca
    67441 tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat
    67501 ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag
    67561 ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat
    67621 cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc
    67681 aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc
    67741 ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat
    67801 cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta
    67861 acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa
    67921 gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct
    67981 aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat
    68041 cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc
    68101 cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag
    68161 atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga aatttttttt
    68221 tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg
    68281 ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac
    68341 ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac atatttttat
    68401 ttttttatat tgttaaaaat tcacaattta ttttttaata gaattatctg ttttccaaat
    68461 tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa attaactgat
    68521 gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa tacaaggcgg
    68581 aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc tgtttttatg
    68641 tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt
    68701 ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg aagaacagat
    68761 cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac atatacctcg
    68821 atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta
    68881 tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca tctctgtcag
    68941 caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg ggatcgaaat
    69001 cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc
    69061 gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg aacgtgccga
    69121 tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag aaaataaaga
    69181 aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg ctgtgacaat
    69241 agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc
    69301 atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct ttattttgac
    69361 actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg aagactttca
    69421 acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag ttgctacctt
    69481 tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg cgctgattca
    69541 tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat tttatctttt
    69601 aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca gctggtccag
    69661 ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg tgatcgaatt
    69721 gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca caaattcgga
    69781 tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg attttcaagt
    69841 tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc agtcctcaaa
    69901 ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg aaaatattat
    69961 tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa acaaataagg
    70021 ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat ttctgtaaca
    70081 atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag gggtccgatc
    70141 tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc tagttggaaa
    70201 atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac aagaatttcc
    70261 aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc gcttactcgg
    70321 tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat tgatagacaa
    70381 acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag tttttcgaaa
    70441 tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt ctttgttttt
    70501 ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg ataataataa
    70561 gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg atatcagcaa
    70621 cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt gtatctgcat
    70681 tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa agtttgaata
    70741 gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa atagcaatga
    70801 tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac tttcttttaa
    70861 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
    70921 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
    70981 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
    71041 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
    71101 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
    71161 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
    71221 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
    71281 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
    71341 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
    71401 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
    71461 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
    71521 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
    71581 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
    71641 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
    71701 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
    71761 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
    71821 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
    71881 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
    71941 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
    72001 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
    72061 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
    72121 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
    72181 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
    72241 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
    72301 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
    72361 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
    72421 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
    72481 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
    72541 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
    72601 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat
    72661 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
    72721 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
    72781 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
    72841 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
    72901 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
    72961 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
    73021 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
    73081 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
    73141 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    73201 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
    73261 gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga
    73321 gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg
    73381 caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga
    73441 agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga
    73501 aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact
    73561 attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac aactatttca
    73621 atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac
    73681 tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt
    73741 aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat
    73801 ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg
    73861 atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg
    73921 gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag
    73981 gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag
    74041 ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc
    74101 acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat
    74161 ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg
    74221 cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc
    74281 tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg
    74341 atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta
    74401 tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg
    74461 ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat
    74521 tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat
    74581 tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc
    74641 cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt
    74701 atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca
    74761 agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag
    74821 gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag
    74881 catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa
    74941 ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga
    75001 tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc
    75061 aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt
    75121 ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg
    75181 ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt
    75241 acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca
    75301 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
    75361 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
    75421 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
    75481 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
    75541 atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac
    75601 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
    75661 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
    75721 attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
    75781 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
    75841 aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc
    75901 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
    75961 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
    76021 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
    76081 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
    76141 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
    76201 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
    76261 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
    76321 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
    76381 ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
    76441 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
    76501 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
    76561 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
    76621 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
    76681 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
    76741 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
    76801 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
    76861 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
    76921 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
    76981 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
    77041 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
    77101 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
    77161 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
    77221 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
    77281 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
    77341 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
    77401 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
    77461 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
    77521 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
    77581 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
    77641 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
    77701 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
    77761 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
    77821 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
    77881 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
    77941 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
    78001 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
    78061 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
    78121 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
    78181 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
    78241 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
    78301 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
    78361 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
    78421 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
    78481 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
    78541 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
    78601 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
    78661 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
    78721 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
    78781 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
    78841 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
    78901 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
    78961 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
    79021 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
    79081 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
    79141 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
    79201 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
    79261 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
    79321 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
    79381 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
    79441 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
    79501 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
    79561 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
    79621 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
    79681 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
    79741 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
    79801 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
    79861 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
    79921 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
    79981 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
    80041 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
    80101 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
    80161 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
    80221 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
    80281 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
    80341 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
    80401 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
    80461 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
    80521 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
    80581 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
    80641 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
    80701 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
    80761 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
    80821 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    80881 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    80941 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    81001 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    81061 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    81121 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    81181 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    81241 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    81301 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    81361 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    81421 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    81481 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    81541 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    81601 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    81661 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    81721 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    81781 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    81841 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    81901 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    81961 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    82021 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa attcactcct
    82081 agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa cagacgatct
    82141 tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa agtttgatta
    82201 catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga gaaatctaaa
    82261 ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc gaccagtaca
    82321 cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa tgattgtcct
    82381 cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc aacttgttct
    82441 tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat accatgtaga
    82501 taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc tagcccttct
    82561 tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat attgtagcgc
    82621 atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat tttttttgta
    82681 tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct tacaagttgc
    82741 atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg atgatagaag
    82801 atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga aatgatggat
    82861 ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc tttgctagaa
    82921 ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc
    82981 tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt aattcccatg
    83041 tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc accaaaattt
    83101 tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga tttacgatat
    83161 tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc tcatcaaatc
    83221 tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga aaaggagtta
    83281 atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt gatagatcag
    83341 aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt tgtaaaattg
    83401 ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg tgacgattgg
    83461 ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta attctttttg
    83521 tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt gaaattgtcc
    83581 aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc tataacttat
    83641 ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac caccacttac
    83701 ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa cagataggat
    83761 cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg caaatactgc
    83821 acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt tttgctcctt
    83881 atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca gattcattcg
    83941 gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa cggatatatt
    84001 ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg
    84061 attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca tagattctcc
    84121 tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa gtgaggaaat
    84181 aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat ccttatcgac
    84241 agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc ttttatggaa
    84301 aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag aaataatagt
    84361 cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc cgcttatttg
    84421 taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt ctacattcgt
    84481 tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag ttactctata
    84541 tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc caatctttgc
    84601 ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat aatcatataa
    84661 aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta caacaatatg
    84721 tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag ttaaagtgat
    84781 taataacaaa acagtcat
//
LOCUS       DACCIQ010000003        77374 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCIQ010000003 DACCIQ010000000
VERSION     DACCIQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 77374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.003"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(249..1814)
                     /gene="guaA"
                     /locus_tag="IVM62_000169"
     CDS             complement(249..1814)
                     /gene="guaA"
                     /locus_tag="IVM62_000169"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6844164.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1873..2796)
                     /locus_tag="IVM62_000170"
     CDS             complement(1873..2796)
                     /locus_tag="IVM62_000170"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6844165.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2992..3597
                     /locus_tag="IVM62_000171"
     CDS             2992..3597
                     /locus_tag="IVM62_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6844166.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3905..4921
                     /locus_tag="IVM62_000172"
     CDS             3905..4921
                     /locus_tag="IVM62_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6844167.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            5062..6363
                     /locus_tag="IVM62_000173"
     CDS             5062..6363
                     /locus_tag="IVM62_000173"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6844168.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6409..7071
                     /gene="deoC"
                     /locus_tag="IVM62_000174"
     CDS             6409..7071
                     /gene="deoC"
                     /locus_tag="IVM62_000174"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6844169.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            7092..7490
                     /locus_tag="IVM62_000175"
     CDS             7092..7490
                     /locus_tag="IVM62_000175"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6844170.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7556..8638
                     /locus_tag="IVM62_000176"
     CDS             7556..8638
                     /locus_tag="IVM62_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6844171.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8850..10412
                     /locus_tag="IVM62_000177"
     CDS             8850..10412
                     /locus_tag="IVM62_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844172.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10405..11538
                     /locus_tag="IVM62_000178"
     CDS             10405..11538
                     /locus_tag="IVM62_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844173.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11538..12494
                     /locus_tag="IVM62_000179"
     CDS             11538..12494
                     /locus_tag="IVM62_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844174.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12593..13759
                     /gene="deoB"
                     /locus_tag="IVM62_000180"
     CDS             12593..13759
                     /gene="deoB"
                     /locus_tag="IVM62_000180"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6844175.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13783..14601
                     /locus_tag="IVM62_000181"
     CDS             13783..14601
                     /locus_tag="IVM62_000181"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6844176.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14618..15325
                     /gene="deoD"
                     /locus_tag="IVM62_000182"
     CDS             14618..15325
                     /gene="deoD"
                     /locus_tag="IVM62_000182"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6844177.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15623..16309)
                     /gene="gpmA"
                     /locus_tag="IVM62_000183"
     CDS             complement(15623..16309)
                     /gene="gpmA"
                     /locus_tag="IVM62_000183"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6844178.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16481..17062)
                     /locus_tag="IVM62_000184"
     CDS             complement(16481..17062)
                     /locus_tag="IVM62_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6844179.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17195..17878)
                     /gene="rpiA"
                     /locus_tag="IVM62_000185"
     CDS             complement(17195..17878)
                     /gene="rpiA"
                     /locus_tag="IVM62_000185"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP6844180.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18197..18610
                     /gene="rpsL"
                     /locus_tag="IVM62_000186"
     CDS             18197..18610
                     /gene="rpsL"
                     /locus_tag="IVM62_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP6844181.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18694..19164
                     /gene="rpsG"
                     /locus_tag="IVM62_000187"
     CDS             18694..19164
                     /gene="rpsG"
                     /locus_tag="IVM62_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP6844182.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19258..21345
                     /gene="fusA"
                     /locus_tag="IVM62_000188"
     CDS             19258..21345
                     /gene="fusA"
                     /locus_tag="IVM62_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6844183.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21517..22704
                     /gene="tuf"
                     /locus_tag="IVM62_000189"
     CDS             21517..22704
                     /gene="tuf"
                     /locus_tag="IVM62_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6844184.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            23111..23419
                     /gene="rpsJ"
                     /locus_tag="IVM62_000190"
     CDS             23111..23419
                     /gene="rpsJ"
                     /locus_tag="IVM62_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP6844185.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23450..24079
                     /gene="rplC"
                     /locus_tag="IVM62_000191"
     CDS             23450..24079
                     /gene="rplC"
                     /locus_tag="IVM62_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP6844186.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            24107..24730
                     /gene="rplD"
                     /locus_tag="IVM62_000192"
     CDS             24107..24730
                     /gene="rplD"
                     /locus_tag="IVM62_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP6844187.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24730..25020
                     /gene="rplW"
                     /locus_tag="IVM62_000193"
     CDS             24730..25020
                     /gene="rplW"
                     /locus_tag="IVM62_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP6844188.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            25059..25892
                     /gene="rplB"
                     /locus_tag="IVM62_000194"
     CDS             25059..25892
                     /gene="rplB"
                     /locus_tag="IVM62_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP6844189.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25935..26213
                     /gene="rpsS"
                     /locus_tag="IVM62_000195"
     CDS             25935..26213
                     /gene="rpsS"
                     /locus_tag="IVM62_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP6844190.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26236..26583
                     /gene="rplV"
                     /locus_tag="IVM62_000196"
     CDS             26236..26583
                     /gene="rplV"
                     /locus_tag="IVM62_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP6844191.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26597..27253
                     /gene="rpsC"
                     /locus_tag="IVM62_000197"
     CDS             26597..27253
                     /gene="rpsC"
                     /locus_tag="IVM62_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP6844192.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27256..27690
                     /gene="rplP"
                     /locus_tag="IVM62_000198"
     CDS             27256..27690
                     /gene="rplP"
                     /locus_tag="IVM62_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP6844193.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27680..27868
                     /gene="rpmC"
                     /locus_tag="IVM62_000199"
     CDS             27680..27868
                     /gene="rpmC"
                     /locus_tag="IVM62_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP6844194.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27893..28159
                     /gene="rpsQ"
                     /locus_tag="IVM62_000200"
     CDS             27893..28159
                     /gene="rpsQ"
                     /locus_tag="IVM62_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP6844195.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28217..28585
                     /gene="rplN"
                     /locus_tag="IVM62_000201"
     CDS             28217..28585
                     /gene="rplN"
                     /locus_tag="IVM62_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP6844196.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28622..28930
                     /gene="rplX"
                     /locus_tag="IVM62_000202"
     CDS             28622..28930
                     /gene="rplX"
                     /locus_tag="IVM62_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP6844197.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28958..29497
                     /gene="rplE"
                     /locus_tag="IVM62_000203"
     CDS             28958..29497
                     /gene="rplE"
                     /locus_tag="IVM62_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP6844198.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29516..29701
                     /locus_tag="IVM62_000204"
     CDS             29516..29701
                     /locus_tag="IVM62_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP6844199.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29737..30135
                     /gene="rpsH"
                     /locus_tag="IVM62_000205"
     CDS             29737..30135
                     /gene="rpsH"
                     /locus_tag="IVM62_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP6844200.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            30167..30703
                     /gene="rplF"
                     /locus_tag="IVM62_000206"
     CDS             30167..30703
                     /gene="rplF"
                     /locus_tag="IVM62_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP6844201.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30864..31220
                     /gene="rplR"
                     /locus_tag="IVM62_000207"
     CDS             30864..31220
                     /gene="rplR"
                     /locus_tag="IVM62_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP6844202.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31241..31741
                     /gene="rpsE"
                     /locus_tag="IVM62_000208"
     CDS             31241..31741
                     /gene="rpsE"
                     /locus_tag="IVM62_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP6844203.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31756..31935
                     /gene="rpmD"
                     /locus_tag="IVM62_000209"
     CDS             31756..31935
                     /gene="rpmD"
                     /locus_tag="IVM62_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP6844204.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31979..32419
                     /gene="rplO"
                     /locus_tag="IVM62_000210"
     CDS             31979..32419
                     /gene="rplO"
                     /locus_tag="IVM62_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP6844205.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32419..33714
                     /gene="secY"
                     /locus_tag="IVM62_000211"
     CDS             32419..33714
                     /gene="secY"
                     /locus_tag="IVM62_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP6844206.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33776..34423
                     /locus_tag="IVM62_000212"
     CDS             33776..34423
                     /locus_tag="IVM62_000212"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP6844207.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34614..34832
                     /gene="infA"
                     /locus_tag="IVM62_000213"
     CDS             34614..34832
                     /gene="infA"
                     /locus_tag="IVM62_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP6844208.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34865..34981
                     /gene="rpmJ"
                     /locus_tag="IVM62_000214"
     CDS             34865..34981
                     /gene="rpmJ"
                     /locus_tag="IVM62_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP6844209.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34999..35364
                     /gene="rpsM"
                     /locus_tag="IVM62_000215"
     CDS             34999..35364
                     /gene="rpsM"
                     /locus_tag="IVM62_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP6844210.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35392..35781
                     /gene="rpsK"
                     /locus_tag="IVM62_000216"
     CDS             35392..35781
                     /gene="rpsK"
                     /locus_tag="IVM62_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP6844211.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35861..36799
                     /locus_tag="IVM62_000217"
     CDS             35861..36799
                     /locus_tag="IVM62_000217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP6844212.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36827..37207
                     /gene="rplQ"
                     /locus_tag="IVM62_000218"
     CDS             36827..37207
                     /gene="rplQ"
                     /locus_tag="IVM62_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP6844213.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37479..38318
                     /locus_tag="IVM62_000219"
     CDS             37479..38318
                     /locus_tag="IVM62_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6844214.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38294..39163
                     /locus_tag="IVM62_000220"
     CDS             38294..39163
                     /locus_tag="IVM62_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6844215.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            39153..39950
                     /locus_tag="IVM62_000221"
     CDS             39153..39950
                     /locus_tag="IVM62_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6844216.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            40153..40899
                     /gene="truA"
                     /locus_tag="IVM62_000222"
     CDS             40153..40899
                     /gene="truA"
                     /locus_tag="IVM62_000222"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6844217.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41354..44023)
                     /locus_tag="IVM62_000223"
     CDS             complement(41354..44023)
                     /locus_tag="IVM62_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6844218.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(44260..44448)
                     /locus_tag="IVM62_000224"
     CDS             complement(44260..44448)
                     /locus_tag="IVM62_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP6844219.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            44718..45080
                     /locus_tag="IVM62_000225"
     CDS             44718..45080
                     /locus_tag="IVM62_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6844220.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            45132..46814
                     /locus_tag="IVM62_000226"
     CDS             45132..46814
                     /locus_tag="IVM62_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP6844221.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            46931..48967
                     /gene="recG"
                     /locus_tag="IVM62_000227"
     CDS             46931..48967
                     /gene="recG"
                     /locus_tag="IVM62_000227"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP6844222.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            49016..50017
                     /gene="plsX"
                     /locus_tag="IVM62_000228"
     CDS             49016..50017
                     /gene="plsX"
                     /locus_tag="IVM62_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP6844223.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            50139..50381
                     /gene="acpP"
                     /locus_tag="IVM62_000229"
     CDS             50139..50381
                     /gene="acpP"
                     /locus_tag="IVM62_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6844224.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            50649..51653
                     /locus_tag="IVM62_000230"
     CDS             50649..51653
                     /locus_tag="IVM62_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844225.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            51654..52598
                     /locus_tag="IVM62_000231"
     CDS             51654..52598
                     /locus_tag="IVM62_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844226.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            52598..53560
                     /locus_tag="IVM62_000232"
     CDS             52598..53560
                     /locus_tag="IVM62_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844227.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            53587..54501
                     /locus_tag="IVM62_000233"
     CDS             53587..54501
                     /locus_tag="IVM62_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844228.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            54527..56308
                     /locus_tag="IVM62_000234"
     CDS             54527..56308
                     /locus_tag="IVM62_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6844229.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            56656..57342
                     /locus_tag="IVM62_000235"
     CDS             56656..57342
                     /locus_tag="IVM62_000235"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6844230.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            57362..60943
                     /gene="smc"
                     /locus_tag="IVM62_000236"
     CDS             57362..60943
                     /gene="smc"
                     /locus_tag="IVM62_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP6844231.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            60952..61761
                     /locus_tag="IVM62_000237"
     CDS             60952..61761
                     /locus_tag="IVM62_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6844232.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            61772..62770
                     /gene="ftsY"
                     /locus_tag="IVM62_000238"
     CDS             61772..62770
                     /gene="ftsY"
                     /locus_tag="IVM62_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP6844233.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            62957..65224
                     /gene="gshAB"
                     /locus_tag="IVM62_000239"
     CDS             62957..65224
                     /gene="gshAB"
                     /locus_tag="IVM62_000239"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP6844234.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKDR
                     RLTMNVLKLLYPEVF"
     gene            complement(65313..65765)
                     /locus_tag="IVM62_000240"
     CDS             complement(65313..65765)
                     /locus_tag="IVM62_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP6844235.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            65915..66865
                     /locus_tag="IVM62_000241"
     CDS             65915..66865
                     /locus_tag="IVM62_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6844236.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            66858..67817
                     /locus_tag="IVM62_000242"
     CDS             66858..67817
                     /locus_tag="IVM62_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6844237.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            67814..68569
                     /locus_tag="IVM62_000243"
     CDS             67814..68569
                     /locus_tag="IVM62_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6844238.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            68592..69545
                     /locus_tag="IVM62_000244"
     CDS             68592..69545
                     /locus_tag="IVM62_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6844239.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(70189..70485)
                     /locus_tag="IVM62_000245"
     CDS             complement(70189..70485)
                     /locus_tag="IVM62_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP6844240.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            70840..71196
                     /locus_tag="IVM62_000246"
     CDS             70840..71196
                     /locus_tag="IVM62_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6844241.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            71206..72105
                     /locus_tag="IVM62_000247"
     CDS             71206..72105
                     /locus_tag="IVM62_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP6844242.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            72622..73353
                     /locus_tag="IVM62_000248"
     CDS             72622..73353
                     /locus_tag="IVM62_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6844243.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            73498..75525
                     /locus_tag="IVM62_000249"
     CDS             73498..75525
                     /locus_tag="IVM62_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP6844244.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            75633..76730
                     /locus_tag="IVM62_000250"
     CDS             75633..76730
                     /locus_tag="IVM62_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6844245.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLSFILLCILLFVLRKKEEKEK"
     gene            77037..>77374
                     /locus_tag="IVM62_000251"
     CDS             77037..>77374
                     /locus_tag="IVM62_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6844246.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSV"
BASE COUNT        25282 a        13929 c        16678 g        21485 t
ORIGIN      
        1 cagatctttt aaatcgaaaa atctgatata tatatttata gtactaaaaa tctgattaga
       61 taaaaagcta attcaatagg aaaataaagg tagtgaataa aagcaaagcc cttgttagta
      121 gttaatctaa caagggcttt ggtagggaaa atggtaggaa accatcttat tttttacgaa
      181 aatcaggaac tttagtaatt gaataatgtt gatttatagg tattcctcat ccatattatg
      241 ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
      301 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
      361 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
      421 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
      481 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
      541 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
      601 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
      661 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
      721 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
      781 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
      841 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
      901 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
      961 ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
     1021 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
     1081 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
     1141 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
     1201 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
     1261 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
     1321 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
     1381 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
     1441 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
     1501 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
     1561 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
     1621 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
     1681 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
     1741 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
     1801 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
     1861 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
     1921 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
     1981 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
     2041 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
     2101 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
     2161 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
     2221 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
     2281 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
     2341 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
     2401 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
     2461 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
     2521 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
     2581 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
     2641 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
     2701 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
     2761 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
     2821 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
     2881 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
     2941 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
     3001 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
     3061 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
     3121 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
     3181 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
     3241 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
     3301 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
     3361 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
     3421 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
     3481 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
     3541 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
     3601 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
     3661 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
     3721 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
     3781 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
     3841 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
     3901 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
     3961 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
     4021 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
     4081 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
     4141 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
     4201 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
     4261 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
     4321 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
     4381 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
     4441 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
     4501 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
     4561 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
     4621 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
     4681 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
     4741 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
     4801 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
     4861 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
     4921 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
     4981 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
     5041 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
     5101 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
     5161 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
     5221 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
     5281 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
     5341 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
     5401 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
     5461 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
     5521 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
     5581 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
     5641 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
     5701 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
     5761 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
     5821 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
     5881 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
     5941 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
     6001 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
     6061 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
     6121 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
     6181 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
     6241 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
     6301 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
     6361 taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac
     6421 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
     6481 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
     6541 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
     6601 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
     6661 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
     6721 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
     6781 attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
     6841 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
     6901 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
     6961 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
     7021 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
     7081 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
     7141 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
     7201 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
     7261 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
     7321 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
     7381 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
     7441 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
     7501 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
     7561 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
     7621 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
     7681 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
     7741 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
     7801 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
     7861 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
     7921 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
     7981 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
     8041 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
     8101 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
     8161 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
     8221 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
     8281 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
     8341 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
     8401 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
     8461 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
     8521 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
     8581 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
     8641 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
     8701 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
     8761 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
     8821 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
     8881 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
     8941 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
     9001 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
     9061 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
     9121 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
     9181 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
     9241 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
     9301 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
     9361 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
     9421 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
     9481 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
     9541 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
     9601 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
     9661 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
     9721 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
     9781 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
     9841 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
     9901 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
     9961 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
    10021 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
    10081 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
    10141 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    10201 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    10261 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    10321 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    10381 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    10441 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    10501 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    10561 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    10621 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    10681 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    10741 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    10801 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    10861 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    10921 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    10981 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    11041 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    11101 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    11161 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    11221 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    11281 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    11341 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    11401 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    11461 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    11521 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    11581 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    11641 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    11701 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    11761 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    11821 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    11881 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    11941 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    12001 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    12061 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    12121 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    12181 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    12241 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    12301 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    12361 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    12421 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    12481 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    12541 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    12601 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    12661 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    12721 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    12781 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    12841 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    12901 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    12961 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    13021 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    13081 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    13141 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    13201 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    13261 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    13321 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    13381 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    13441 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    13501 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    13561 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    13621 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    13681 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    13741 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    13801 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    13861 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    13921 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    13981 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    14041 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    14101 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    14161 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    14221 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    14281 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    14341 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    14401 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    14461 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    14521 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    14581 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    14641 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    14701 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    14761 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    14821 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    14881 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    14941 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    15001 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    15061 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    15121 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    15181 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    15241 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    15301 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    15361 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    15421 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt
    15481 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    15541 gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac
    15601 agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat
    15661 ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag
    15721 agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa
    15781 cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt
    15841 ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac
    15901 gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt
    15961 aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt
    16021 tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta
    16081 cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa
    16141 cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt
    16201 ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca
    16261 atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc
    16321 cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg
    16381 gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa
    16441 aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga
    16501 tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg
    16561 aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg
    16621 tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc
    16681 caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt
    16741 ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca
    16801 gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc
    16861 gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca
    16921 aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac
    16981 aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc
    17041 gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct aactggatat
    17101 aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct
    17161 gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa
    17221 tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat
    17281 gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga
    17341 cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt
    17401 cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg
    17461 agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag
    17521 aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca
    17581 aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta
    17641 ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg
    17701 cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca
    17761 tacggcgacc aatctcttct accatataat aggcggttga accggtcccg agacctacga
    17821 tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct
    17881 ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta
    17941 cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct
    18001 cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc
    18061 tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat
    18121 ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg
    18181 aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa
    18241 ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca
    18301 aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc
    18361 taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga
    18421 agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt
    18481 acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt
    18541 agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc
    18601 taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta
    18661 ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa
    18721 cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt
    18781 gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg
    18841 atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt
    18901 caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt
    18961 gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc
    19021 ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac
    19081 aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt
    19141 gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc
    19201 tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg
    19261 accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct
    19321 ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt
    19381 gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt
    19441 acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac
    19501 acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt
    19561 gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
    19621 caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt
    19681 gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca
    19741 atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg
    19801 aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa
    19861 gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact
    19921 gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa
    19981 gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca
    20041 tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg
    20101 ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct
    20161 gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta
    20221 ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa
    20281 aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct
    20341 cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac
    20401 acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa
    20461 ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa
    20521 atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac
    20581 gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt
    20641 gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac
    20701 cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt
    20761 aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc
    20821 gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa
    20881 actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc
    20941 aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt
    21001 gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa
    21061 ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac
    21121 gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt
    21181 gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct
    21241 actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca
    21301 gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct tgaaaccgga
    21361 tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata
    21421 attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat
    21481 aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt
    21541 tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta
    21601 acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat
    21661 caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt
    21721 gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac
    21781 gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct
    21841 gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt
    21901 ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa
    21961 ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt
    22021 cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa
    22081 attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac
    22141 aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct
    22201 acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt
    22261 gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac
    22321 tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc
    22381 caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca
    22441 gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac
    22501 cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga
    22561 actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc
    22621 gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt
    22681 gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg
    22741 gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc
    22801 aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag
    22861 tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt
    22921 gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt
    22981 aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc
    23041 acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg
    23101 agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat
    23161 tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc
    23221 tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa
    23281 atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt
    23341 gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa
    23401 catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg
    23461 aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc
    23521 agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga
    23581 tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc
    23641 tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa
    23701 aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc
    23761 aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa
    23821 acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg
    23881 gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat
    23941 gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa
    24001 tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc
    24061 agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc
    24121 attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg
    24181 aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag
    24241 acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg
    24301 gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg
    24361 aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt
    24421 tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc
    24481 aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa
    24541 cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt
    24601 atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga
    24661 cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt
    24721 gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg
    24781 cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta
    24841 gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac
    24901 gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa
    24961 gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa
    25021 gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac
    25081 ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa
    25141 cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc
    25201 gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca
    25261 aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt
    25321 cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta
    25381 aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa
    25441 atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac
    25501 ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag
    25561 aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat
    25621 gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag
    25681 ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta
    25741 atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt
    25801 ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca
    25861 aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg
    25921 agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa
    25981 tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac
    26041 gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat gatggacgta
    26101 aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac
    26161 ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg
    26221 ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt
    26281 cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca
    26341 gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta
    26401 ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta
    26461 gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa
    26521 ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa
    26581 taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat
    26641 ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga
    26701 tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga
    26761 aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt
    26821 tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg
    26881 taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt
    26941 aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca
    27001 agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg
    27061 tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca
    27121 tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact
    27181 aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa
    27241 aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga
    27301 aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa
    27361 gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact
    27421 cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact
    27481 gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca
    27541 ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt
    27601 gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag
    27661 gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat
    27721 gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac
    27781 aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa
    27841 aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga
    27901 aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac
    27961 aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta
    28021 ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag
    28081 aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga
    28141 agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg caaaatctga
    28201 aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg
    28261 cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat
    28321 cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg
    28381 tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc
    28441 atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg
    28501 aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt
    28561 ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt
    28621 tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga
    28681 aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt
    28741 cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga
    28801 agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt
    28861 tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt
    28921 agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata
    28981 tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac
    29041 acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa
    29101 aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac
    29161 aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa
    29221 agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact
    29281 accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta
    29341 tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa
    29401 agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga
    29461 gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc
    29521 taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac
    29581 tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat
    29641 ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta
    29701 aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca
    29761 gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct
    29821 gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat
    29881 gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa
    29941 aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat
    30001 gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact
    30061 tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc
    30121 gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg
    30181 aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac
    30241 agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat
    30301 cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca
    30361 tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa
    30421 agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt
    30481 gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt
    30541 accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc
    30601 tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt
    30661 tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc
    30721 atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt
    30781 tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca
    30841 ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa
    30901 agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg
    30961 tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc
    31021 tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac
    31081 aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct
    31141 ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg
    31201 aaaatggact agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa
    31261 acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg
    31321 tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg
    31381 atttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc
    31441 gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg
    31501 tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc
    31561 tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc
    31621 tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt
    31681 gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata
    31741 aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt
    31801 cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg
    31861 gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac
    31921 gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat
    31981 gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg
    32041 tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg
    32101 ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt
    32161 accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt
    32221 attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat
    32281 cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt
    32341 aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg
    32401 atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt
    32461 agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt
    32521 actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac
    32581 atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt
    32641 tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga
    32701 tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga
    32761 tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa
    32821 tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg
    32881 attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt
    32941 atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt
    33001 atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct
    33061 gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt
    33121 ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct
    33181 acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct
    33241 agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct
    33301 gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg
    33361 atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct
    33421 gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt
    33481 aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc
    33541 ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa
    33601 tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg
    33661 aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc
    33721 gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa
    33781 cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga
    33841 tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga
    33901 aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga
    33961 agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt
    34021 attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga
    34081 tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga
    34141 acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa
    34201 ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga
    34261 agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc
    34321 aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat
    34381 cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc
    34441 gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc
    34501 cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt
    34561 ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa
    34621 aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta
    34681 aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc
    34741 attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta
    34801 accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat
    34861 tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg
    34921 taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata
    34981 attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt
    35041 gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta
    35101 gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat
    35161 gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac
    35221 ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga
    35281 ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc
    35341 gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca
    35401 aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc
    35461 cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg
    35521 tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct
    35581 caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa
    35641 gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca
    35701 ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct
    35761 ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt
    35821 gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc
    35881 aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga
    35941 aagagggtat gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc
    36001 aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa
    36061 aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat
    36121 gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc
    36181 tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag
    36241 tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt
    36301 tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat
    36361 ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga
    36421 agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc
    36481 aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga
    36541 tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt
    36601 ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc
    36661 aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg
    36721 taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg
    36781 tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa
    36841 actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt
    36901 aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga
    36961 aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt
    37021 cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc
    37081 tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac
    37141 acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact
    37201 tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt
    37261 tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga
    37321 gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt
    37381 ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat
    37441 agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag
    37501 aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct
    37561 ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga
    37621 cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa
    37681 atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga atggtttttc
    37741 aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg
    37801 aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag
    37861 ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac
    37921 gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa
    37981 ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag
    38041 aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca
    38101 atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt
    38161 tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga
    38221 agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga
    38281 tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac
    38341 accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac
    38401 tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct
    38461 tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga
    38521 taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc
    38581 acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt
    38641 cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga
    38701 aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat
    38761 cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact
    38821 tgatccccag ggcagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca
    38881 gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt
    38941 ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa
    39001 cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga
    39061 attaatggct aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga
    39121 cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt
    39181 atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt
    39241 tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat
    39301 ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca
    39361 ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg
    39421 gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg
    39481 gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta
    39541 ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg
    39601 tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta
    39661 cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg
    39721 gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg
    39781 tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag
    39841 gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta
    39901 tcattgcttt tgtattatta actgtgctac tgatcttttt aagaggataa acgttcagtg
    39961 ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcaa gcagtgtatg
    40021 acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa
    40081 agaaagaacg aaaaaatttt atagaaaaat cattattcca gataaccttt taggaacaaa
    40141 ggagagaaaa acatggtacg ttacaaagca atcattgcgt acgacggaac aaattttaat
    40201 ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa
    40261 aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta
    40321 catgcgatcg ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg
    40381 cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt
    40441 tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat
    40501 atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttacccactt
    40561 gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct
    40621 ttttgtgcat caggaagctc gatagaggat aaaatacgta cgatctacga ggctaagatg
    40681 gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat
    40741 aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa
    40801 gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacac
    40861 cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttatgat agaagtggtc
    40921 agcttcaaga aataagaagg tatttacgaa aattacttct caaaattttg caaaatttcg
    40981 gcttattttc gaagaagcta cttctggaac accgtttaag gaagaggtcg tgaaatcagc
    41041 gttttgacct gagtattaga gaaacaaacg ggaaaatagc tcctcatatt ttgccgtttg
    41101 ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg
    41161 tcatttaact ctgagtcaca aagagcaatt tcaaaaaaca gcttctcatc tttgatgaat
    41221 atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct
    41281 gtaaccaaaa aacgatggaa cgttgttttg attgcccttg gtgcggacag tctgaacagg
    41341 ttccatcgtt tctttatttt ctacgtcttc ttctgtgtgt gtttcgtggt tgttcttcta
    41401 ttgctggatc aatcgtgcca taaaaggggc gattgcggtc aaggtaccag acataaagaa
    41461 taaacaatgc aatacatagg acaaaaagga ttgcacctat cgtaaatcct tcaggcaagt
    41521 aactgaaagt gacagtatgt ttacctgctg gaacttttat catcaagaat gcgtctttga
    41581 aattgctgac cttgactggt ttcccatcaa ttttagcttt ccatcctttg tcataaggaa
    41641 tagttgtcac caacatggaa tcttcagcgg tatttacagt cccagaagct tttctgccag
    41701 tggcttccat ttcgacacct ttttctttga tctgattcat tgcggcttga taggctacaa
    41761 catctagacc aacgacttta ggctccatga aacttatttc tttcgtccca taaaaacttg
    41821 cagtaaatgt cactgttgtc tcttgaggat aatagccaag atcatagtat tgaccagaaa
    41881 tgttgatttg cgtttttcgc gatgtaccat tgacagtgat cgtcgcacta gaactttcta
    41941 attgcgcaaa attggttggg aacaaactta agtaagcttg tgtatttgca ggcacatgga
    42001 cttcccaagt gagctctttt ggctggttat ctgctgtttc tgagtaagtg acgccagtcg
    42061 ttgtctttgt gatcgtcgta ttcaactgat tcttcaaagt aggtgtatag aatgtaaaat
    42121 actgttcatt tagaccagac aatgtattga tcaagtttgt ctgactggtc aaattatctg
    42181 tcactggctg ctcaacatca ttaacggaca acggtgcagt aaatgctagt ggcaatgcat
    42241 tgacattctg atacagtgta tagtcgccgc tttgaccagc ttggcggaag ccatatttac
    42301 tgaacgtgct ttcgttttta gaaatgttat atttgattcc tgtaaaccca tccatcagca
    42361 aagtattatt gttataacgg atattcaaat ttgtccctct tgaacggaaa cctaatttat
    42421 ccaggtaaga ggaagaatta cgatttctta tagaagaaaa caaactgata ccactgtaac
    42481 catagttgat cgaatcgttt gcagatactg gatccagatt ttccaagcgg taaaacgagt
    42541 cggaatcttt ttttgtttca tttacaagag ctttcaagtc aggataagga tcagtatata
    42601 aactgcggga agcataattc caatcatcaa gaatgccatg gatcatactg ttcgtattga
    42661 ccagtgcttc agcagacatg atcaataata acaatatact cagatgattg aatgaaagtt
    42721 ttttatgctg ataaaatccc acagcaaaag cataaatccc taagaaaacc actgtcaata
    42781 caaaggaagt catcgtcaca taggtgtagc ttccttcagg tttgatgccc catgccaccg
    42841 caaagagagc agctacgacc aaaatgattc ctaacaacag cccacgatcg tcttgttcta
    42901 tcttttccca tccttttcct gcaagcagga gcactaagaa agagaacaga taggcataac
    42961 ggaataaaaa catattcggt gcatgcatac catgccagaa caaattcaat ggaacaagat
    43021 aaaaacttgc gataagtatg gcgaaaagac tgccgtacaa aaatttattt ttcaacggta
    43081 ctttccgact ggcaaaataa aacagacaga ggactaatgg tagcagacct gcgtaaacga
    43141 acggaatcga tccgtatttt gtcgtgtcat aaacaccaat catatttttc atgattagat
    43201 ccagataaga agtcgcttct gttttcaacg tagtgatctg actcagtgcc tcgccatttg
    43261 accgaagatc cagcactgct ggcaatacaa tgatcattga agcgccgcct gccagtaaag
    43321 aagtgatacc ataaggaaca atcgtttttt tatattctga ccagtttgtc aatagacggg
    43381 cgataaaata taagaaagaa aatacgccaa tcataaaacc catataaaaa ctggataaaa
    43441 acaagagtaa ataactgata aataaaagtt tcggccgttt aagatccata acacgatgaa
    43501 tccccaaaat cactaatgga agataaaaaa atgcatccag ccacatgatg atctccgaat
    43561 gtgcagtgat aaatgagatc aatgcgtaac tgacgctaag tgcaacatgc ccccagcgag
    43621 gaatcttgaa cgtacgtgca agaaaccaaa aagccaatcc ggcacagccg accttaagca
    43681 aagtcagaac atacagtgca tctggcatca tctggtttgg aaaaaagaac accagtggcg
    43741 tgaagatccc gcctagatag taagaaatta aagataaata attcaatcct agagaagcat
    43801 tccatgtata aaaaatactt tgctttccaa gaagaacatt tcggaagctg gcatgaaaat
    43861 tagaaaactg tgaaaaggca tcacttgcca aaatacttct gctgcttcca ggatagattc
    43921 cgatacttag ataaacaagt gctaaaatca aaaacggaat caggaaacta gctgctgcgt
    43981 agcgtccgtt ttctctaaag aatgacttta aatttttttt catatttatt ttccaccaaa
    44041 tttctatgaa gttgtattca gtttatcata tcctgttttt ctctgcttaa aaagagtcta
    44101 gttttcacaa aaaaataaaa tttagaataa ataacttcat aatttgttcg tataacaaaa
    44161 ataaacgcaa aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt tattaaataa
    44221 aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc
    44281 tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg
    44341 aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt
    44401 gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc
    44461 ctttgccttg atttggagca atagaataat tcagctcata ccagactaac atatcatata
    44521 gaatgggaat ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt
    44581 aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc
    44641 ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc
    44701 gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta
    44761 ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag
    44821 gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt
    44881 actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga
    44941 ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat
    45001 acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg
    45061 ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga
    45121 ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg
    45181 caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg
    45241 tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag
    45301 tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc
    45361 tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta
    45421 agcaaattcc agatgttgtc tcattaaatg cttctgatct tgctgcagcc ttcactcatg
    45481 gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag
    45541 ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag
    45601 taatgacagc tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca gacttgcttc
    45661 cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg
    45721 agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc
    45781 cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg
    45841 cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac
    45901 ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag
    45961 attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc
    46021 caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca
    46081 acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga
    46141 aaaaagaaat accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc
    46201 aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtggacaa acaatgaatc
    46261 caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct
    46321 tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc
    46381 cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca
    46441 atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg
    46501 tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag
    46561 aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag
    46621 caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct
    46681 tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa
    46741 aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat
    46801 tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag
    46861 gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aaaggaggga
    46921 ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa
    46981 aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta
    47041 tccttttcgc tacgaagata ttcaggaaag aaatctaaac gagatccagg atcaagaaaa
    47101 ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa
    47161 aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa
    47221 tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg
    47281 ggatgcaaag cgtaaagcct tgaacgggat gaagatttta ggttctcaaa caatggatga
    47341 cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat
    47401 caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt
    47461 ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga
    47521 ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca
    47581 aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct
    47641 ttatgatgtc caaaaattga aacaatttac tcaaaagcta ccttttgaac tgacaaatgc
    47701 acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg
    47761 acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc
    47821 taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca
    47881 acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgattac
    47941 ggggtcaacg aaagcaaaag aacgacgtga gctccttgaa aaactagagc aaggggaaat
    48001 taacgtcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg
    48061 tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga
    48121 aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc
    48181 tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat
    48241 cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa
    48301 aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga
    48361 agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc
    48421 aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat
    48481 ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg
    48541 ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc
    48601 acagctgcac caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt
    48661 agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac
    48721 caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg
    48781 cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt
    48841 tttggaggta gccagagaag aggctgcagc cctttggcaa ataaaggatt ggcagatgct
    48901 tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt
    48961 cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa
    49021 aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc
    49081 tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat
    49141 caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa
    49201 tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc
    49261 tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc
    49321 gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt
    49381 gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg
    49441 cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta
    49501 tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga
    49561 agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag
    49621 cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt
    49681 ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa
    49741 tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc
    49801 tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg
    49861 cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc
    49921 cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg
    49981 acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcgatcc
    50041 gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta
    50101 ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta
    50161 acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg
    50221 aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag
    50281 agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag
    50341 tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga
    50401 aggataaaaa gctgcatgca gctttttatc cttctttttt taatcttttt gacacgtaaa
    50461 agtcattttt ctgataattc tggcgataaa cagaaattat gttgcaaagt aaagcggttt
    50521 tctatataat tattaataat tcattacaca aagataagaa taacctttgt tataaaacga
    50581 tttattctgt gtaatgtcct tttttattaa gaaaaacttc catttaggaa gcagagaggg
    50641 gtattcgatt gtccgataac caattattag aagttcagaa cctacataca ggattccgac
    50701 tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac aaaaacgaga
    50761 ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact acgattatgg
    50821 gattacttga tcctaataac acaaaaatta caggtgagat tttatataac gatctgaatt
    50881 taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc ggaatgattt
    50941 tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag atcaaagaag
    51001 gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca ctagaattgc
    51061 tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc catgaactat
    51121 caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg
    51181 tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag attcttgatt
    51241 tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact catgacttag
    51301 gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag ttcgttgaag
    51361 tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc tctttactga
    51421 actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc
    51481 cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt ccatggatac
    51541 cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac cacttggtac
    51601 gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg tagatgacag
    51661 aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga ttcttcaatc
    51721 gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa aaaggaaaga
    51781 catatggttt agtgggagag tctggctctg gaaaatctac tacgggaaaa gccgttgtgg
    51841 gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa
    51901 gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa gattcaatgt
    51961 ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc cgaaattttg
    52021 aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat atcgttggga
    52081 tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa cgtcaacgtc
    52141 taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat gaacctgtat
    52201 ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga atccagcaag
    52261 aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg
    52321 acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga gaagacattt
    52381 ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg caaatcgatg
    52441 tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag tatatccaaa
    52501 accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg atcacaacta
    52561 cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga ttctgcgacg
    52621 tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct tattagccca
    52681 agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc cggcagtgat
    52741 cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt acttccgttg
    52801 gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac gctctgttgc
    52861 tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga cagttgtcct
    52921 tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa attcaatctt
    52981 ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat ttatcttcgg
    53041 cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa gtggatcaca
    53101 aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga aatatattct
    53161 gttaccatca attacccaag catttttggc gactgccgtc acaatccaat atctgcgaag
    53221 tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca aaggggtacc
    53281 gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga tggcttctca
    53341 aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa agatttttgg
    53401 ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact ataccgtgat
    53461 cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac tttcagatat
    53521 catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag aaaggagaat
    53581 ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc tatggggata
    53641 cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt aggtttgcta
    53701 gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga caaagtgatg
    53761 tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca aggacaaaag
    53821 ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat cataggtgct
    53881 agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat cggggttgga
    53941 ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat gcggatcgtt
    54001 gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc gattatttcc
    54061 agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt agctaaagcc
    54121 cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag tgcctcaaaa
    54181 acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca
    54241 ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga gacaactttg
    54301 actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt gatcggttat
    54361 gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc atcaatcctt
    54421 attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg ttctgcagat
    54481 gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga agaaaacgat
    54541 ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac
    54601 agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat taacttttcc
    54661 aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag ctgcggtcgc
    54721 aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt catatgatgc
    54781 agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt tcagaatcac
    54841 agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga caatcaaagt
    54901 aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg tgattttccc
    54961 ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca ctttccgtaa
    55021 catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg gaatcaaaaa
    55081 aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca tgcttcaatc
    55141 gggtggtggt atctggtcat atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa
    55201 agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc cttattacat
    55261 gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact actggaatgg
    55321 tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg caactgaagc
    55381 attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt acgataccta
    55441 taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata cttatctagg
    55501 attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc cagattcaaa
    55561 aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata atgcagtagg
    55621 tgaacgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc cagtattcca
    55681 gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag cgaaaaaact
    55741 attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag acaaagatgg
    55801 aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc aaccattggc
    55861 agattactat atccaacaat ggaaagaaat tggtttggat gtccaataca caacaggtcg
    55921 tctgatcgaa ttcaattctt tctatgataa attagaaaac gatgatcctg aaattgatgt
    55981 ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat ggggaccaaa
    56041 tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga tcaacgatat
    56101 cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca
    56161 atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag tattgccagt
    56221 caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat gggcaaatgt
    56281 ttctgttaca gcagattcac gttcataatc actaaaaatg accgaaaaag ggcgatgtcc
    56341 tttttcggtt ttttgatatt tttctgttta tttctctaat attcaggtca aggcgctgat
    56401 ttcatgactt ctccaataaa cggtgttcaa agtccagttt ctcggtatta agtcaaattt
    56461 atgcaaaaat atggaaagcc attttcacaa aattttttct tcatactcga aactaaacga
    56521 atttttcaca acttctcgtc aatcactagg attattaaag ctttacttac cttttgaccg
    56581 aaccatttca aatttgacaa aaaaccagta taataaggaa gtatcgaaaa aatatccata
    56641 aaaggaatgt ggaaaatgga caaccagctg acaaaagaac taaaagaaaa ctataacatc
    56701 gtttttcacg atctaaattt gcttgaacaa gcttttaccc attcatccta tgtgaatgag
    56761 catcgcaatc tgcaattatc ggataatgag cgacttgaat ttttaggaga tgccgtttta
    56821 gaactgatgg tttctcagta tttattccgt ttatatcctc atttgccaga aggaaaattg
    56881 acaaaaacac gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt
    56941 cactttgatc aatatgtttt gttgggcaag ggagaagaga attcaggagg acgtacgcga
    57001 ccagcattgc tttgcgacct attcgaagct tttttaggcg cgttatatct tgaccaggga
    57061 ttcgacacga cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct
    57121 ttttcacatg agatggatca taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat
    57181 gtatcaatcg aataccgatt gatcaatgaa gaaggtccag ctcatgaacg agtattttgg
    57241 atcgaagttt atgtagatga tcaattgatt ggcactggac aagggaaatc gaaaaaatta
    57301 gcagaacaag ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt gagggaaagc
    57361 tttgtatttg aaacgaattg aaatagccgg ttttaaatca tttgcagaca agacagtcat
    57421 tgattttgaa aatagcgtca cagcagtggt tggaccaaat ggcagcggaa aaagcaatat
    57481 tacagaggct atccgctggg tattaggaga acaatctgct aaaagtctgc gaggcggaaa
    57541 aatgccggat atcatctttg ccggttccga tacccgtaag cagctgaata ttgcagaagt
    57601 cacagtgatt ttagacaata gtgatcatta tctgccattg gagtacaatg agatcagtgt
    57661 aacccgccgt tatcgacgga ctggggaaag cgagtttttc atcaataaac aaagctgcag
    57721 attaaaagat atccaagaat tgttcttaga ttctggatta ggaaaagaat cgttctcaat
    57781 catttctcaa ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat
    57841 ctttgaagaa gcagctggtg tcttgaaata caagcagcgg aaaaagaaag cagaacagaa
    57901 gctttttgaa acagaagaca acctcagccg cgtccaagac atcatccacg aattggaaga
    57961 acagttaact cctttggccg cccagagtga ggcagcaaag gagtttttac ggctaaaaga
    58021 aacactgact caaaccgatg tctctttgat ggttgctgaa atcaagacag caaaaaaaga
    58081 ttgggacaac aaacaagcac agcttgcgaa attcaatcta gaattaggca aactatctga
    58141 gtcgatccaa gaacaagaat ccattttggc gaagcagcgt aaagaaaatg cgcaagccga
    58201 tcgtctgatc gaaaaaaatc agcaagtatt attagatctt tcggaaaaac tgaagcagac
    58261 agaaggacaa aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga
    58321 atatcaaact tcattagcag aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga
    58381 aagcttgatg aaagcagcag cagaaaaaga aacagagatc caaaaagctg aagcaaatct
    58441 cataaaaaca cagcaagaat tagaaaaata ccaaaaatca acgaaagaat tattggcaga
    58501 attaagagat caatatgttg atttgatgca agaacaagca gctgtaggaa acgaactgaa
    58561 gtatctagaa cgtcaatata tacaagaaac agcaaaaagc aagcagacgt tagccaagca
    58621 atcggaagta gaagcatcag tagaccgatt gatcttacaa aaagaagaat tgacacaaaa
    58681 gcaggcgcag ctaaaatcat ctttaactga aacaaaagag aagttagaaa tgatccaaca
    58741 aaacgggaaa aagttccaag aagcattagc aaaagaacag cctaaaatgt atcaattaat
    58801 gaatcaagtc cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta
    58861 ttttggtttt taccaagggg ttcgattggt cttgcagcat aaacagcagc tttcgggtat
    58921 tgtcggagca gtggctgaac tgatcgatgt tcctgcagat ttcacgttag ctatcgaagc
    58981 agctttaggt ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc
    59041 catcacgtat ttgaaacagc aaagaggagg aagagcaaca ttccttccac tgacgacaat
    59101 caaaccaaga caattaccgg ctcatatatt gacacaagca gcagctgtag aagggtttat
    59161 agggattgcc agtgaacaag tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct
    59221 gctaggaacg atccttttag ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat
    59281 ccgctatcaa tatcgagtgg tttccttaga aggtgatgtg atgaatgccg gaggatccat
    59341 gacaggtgga gcgaataaac gaggaaacca aggcagtttg tttgtacaaa atcaagaatt
    59401 gaaacagttg actagtgaat ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa
    59461 agttcaggag ctgcaacaag aaacagctcg tttagccgaa gaacaagaag ttttgcgtac
    59521 acgaggcgaa caattacgtt ttgaagaaca agaagcgaca aatcagctac aaaacatcat
    59581 aaatgaactt gaacgttttg aaaaagaaaa gcaaatctca acttttgaga cacgtgaact
    59641 gcagcagttt atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga
    59701 tcttgaatcg caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga
    59761 ccagatggaa gccagaagag cacaagtaca aagtcaaaaa gcacaagaac aagcagatct
    59821 tgctgtcttg aaagagcagt ttaatcatct gcagatccag ctccgcggtg cccgagtcca
    59881 aaaagcagag gcaaccgaac gtcaagaagc gatagaaaag cagttggcga cattgacagc
    59941 agacttttct gatcatgaag tcacagaaga aagtttggaa aaacaaatta acgaattatc
    60001 tgctcagcga gaaacattaa aagccgagtt ggcaaaagcc aaagagcagc gagatagaag
    60061 gcaaaaggag atcgataagc ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc
    60121 acgactgtcg gaacaatcca aattagaagt ccaaaaagac cgagctgaaa tgattttaga
    60181 taatcatttg acctatctgc agtcagaata tcagctgaca tttgagaaag cttcccaaga
    60241 ctatcaagaa acaaccgata tcgaagacag tagaacaaag gtttcatcct taaaagaaca
    60301 gattgaaaaa ctgggaccag taaacttaaa ttcaatcgaa caatacgaac aagtcagtga
    60361 aagacataca ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa accaattgtt
    60421 tgaaacgatg gatgagatgg atgatgaagt gcggacaaga ttcaaagaag tatttgaagc
    60481 tatccgtcaa gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta aagctgaatt
    60541 agtactgacg gaaccaactg atttgctgaa aacaggtatt gagattgaag tacaacctcc
    60601 tggcaaaaaa ctgcaaagtc taagtttgct ttctggagga gaacgagcac ttacagccat
    60661 cgcgttgctc ttttccatta tccgtgtctg tcctgttccg ttttgtatct tagatgaggt
    60721 agaagcagcg ttagatgaag ccaacgtgaa acgttttggt cgttacttga gcgatttcca
    60781 agatgacaca caatttatcg tagtcacaca ccgcaaagga acgatggtcg cagcagatgt
    60841 actgtacgga gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg tacggatgga
    60901 agatatcaat gaagaggcga aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa
    60961 ttagtagcaa tcgatttgga cggtacatta ttaaatgaac aaaagctgat ttcagatgag
    61021 aataaacaag cattagcaca agcaaaacag caaggggtca agatcgttct atgtacagga
    61081 cgccccttag ctgcgatggc gcattatctg caagaattag gactggtaga tgaaggtgac
    61141 tacagcatca ctttcaacgg cggtttggta caaaaaaatg atactggtga aatcatggaa
    61201 aagaaagtga tggaagtatc agatatccaa cgcttataca aactggctca gcaattggat
    61261 ctgccactag atgttttgtc tgacaatgtg gttttacagc tgcctagtgc cccaaggaag
    61321 caatccttgt acaatgtgtt gaataagctg ctgcaattcc aaccagcgga tataagcgat
    61381 attacagatg aatttgtgct taataaggca gtaatcgctt atgaccaaga agaattagat
    61441 cctaaaatca aagaaatacc agcagaattc catgaaatgt atgagatcat caaaacaaga
    61501 agtatgcttt tggaatttat gccaaaaggt gttacaaaag cttatggtat ttctttattg
    61561 gcaaaagatc ttggtttgaa acaagaagaa atcatggcga tcggtgatga ggaaaatgat
    61621 ttaccgatga tccaatatgc tggactaggc gtagcgatgg agaatgctgt gcctttcgtc
    61681 aaagaagcag cagatttcgt gacagccagc aatgaagaaa atggcgtagc caaagtgatc
    61741 caaaaatttg tattagagta gggggagtca aatgggattt tttgataaaa tcaagcgtgc
    61801 ttttactggc gaagagaaaa cagaagaagt tgaagtacag gagaagtacg acaagggttt
    61861 agaaaagacg cgtaaaacat ttggtgaacg gatgaatgag ttgtttgcca attttcgaac
    61921 agtcgatgaa gacttttttg aagaattaga agaaacactt atcggtgcag atgtaggttt
    61981 tgaaacagcg atacgaatca ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa
    62041 aaaatcggca gaagtccaaa atgcgatcat cgagaaactt gtggatctct atgaagcgga
    62101 aggaatcaat gaagtaaatg aactaaatat ccaagaagac ggactaacag ttattttgtt
    62161 tgtcggtgtt aatggtgtag ggaaaacaac aagtattggt aaacttgccc atcagttcaa
    62221 aaacgaaggt aaaaaagtat tgatggcagc agcagatacc ttccgcgctg gagcaatcga
    62281 ccagttagtt gtctggggcg aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg
    62341 cgatccagca gctgtcgtat tcgatgcttt ggaaagagca aagacagagc aggcagatgt
    62401 tttactagtt gatactgcag gccgactgca aaataaagtg aacttgatga acgaattaga
    62461 aaaaataaaa cgagtaatcc agcgggaatt accagaagca ccccaagaaa cacttcttgt
    62521 attagatgca accactggtc aaaacgcgat ggttcaagca aaacaattca aagaaacaac
    62581 agatgtgacc ggtcttgttt tgacaaaatt agatggtaca gcaaagggcg gtatcgtact
    62641 tgctatccgt aatgaattgc atttaccagt gaaattggtc ggtcttggtg aaggaatcga
    62701 tgacttggag ccgtttgatc caaatgattt tgtcgttgga ttgttcaaag gattactaaa
    62761 agaagaataa cccatgtcta aaaagcagga gctgtgacaa aagttgcagc tccttgtttg
    62821 aatgattgtt tttaacatag ctgtgttttt gatgccttca tttttatact tatttgtaag
    62881 cggttctcaa acaatttcgt tctttttctt agactttttg attataatta gattcatatg
    62941 aattggaggt cacttaatga tgaattttaa gcaattatta ttgcatgtca atgcgcgccc
    63001 ttttatcgac caagctcgat ttggtataga acgggaagga cagcgggttg atcttgcagg
    63061 aaatctagca aaaaccgatc atccagcaat ctttggcgat cgatcctatc acccctatat
    63121 ccaaacagat tttagcgaaa cacaaacaga gatgatcacc cctgttaccg attctattcc
    63181 cgaattattt cagtatctcg ctgctgttta tgatgtgact gctcgttcta taccgaaaga
    63241 agagatgatc tggccattaa gtatgccacc tgccttaccg gaaaaagacg aagagatcat
    63301 tattgcaaaa ttaaaaaatt tcgaagatgt cttgtatcga cgttatttag caaaagaata
    63361 cgggaagcga aaacagatgg taagcggtat tcatttcaat tttgaatttg gcgatgagtt
    63421 gctaagaaca ttgttcagcc atcaggaaga atttcaagat ttttccgaat tcaaaacaga
    63481 actttatttg aaaacagcga gaaactttat gcgctatcgc tggatgatca cttatttatt
    63541 cggtgcttca ccaatgagtg agaagaacta ctttttagat gaatcacatc cgcaagaacc
    63601 tgttcgcagt attcgcaaca gtgcgctggg ctatacgaat catccaaatg tgaaagtttc
    63661 ctatgcttct atgaaacagt atttagcaga tatcgagcga atgatcgaag aagggaaact
    63721 ttcggaggag aaagaatttt atacaccgct tcgtttccga ggcggaaaga aagtcgcaga
    63781 tttagcaaca acaggtgttc gttatattga attgcggaat atcgatttga atccttatgc
    63841 aagattgggt atcaatccag agcaagttcg gttcttacaa ttattcctga tgtacatgtt
    63901 atggacagaa gaaaaagaag actgtgacca atgggtagca gaaggtacga ctcgaaataa
    63961 caaagtagca ctagaacaac catcagatca aacagaattt catcaagaag gtagagaaat
    64021 tcttgaagga atgaagcaaa tgctggttga attggattgg ttggattctc tttatttagt
    64081 agaggaagca ttgactcaaa tggatcatcc tgaacaaacg ttagcagcga aactctatca
    64141 agaagcacag ctatcgagtc agcaagaagt tgctgtggca ttgggacatc aatattataa
    64201 agaaagccat gaacgtccct atcagttggc cggttttcgc gaaatggaac tttctactca
    64261 gatatttatg ttcgatgcca tccaaaaagg tgtccaagtc aaagtattag atgagtcgga
    64321 tcaatttttg cgtctgcaat tccaagacca tgtagaatat gtgaaaaatg caaacatgac
    64381 aagtaaggac agctatatcg tcccacttat catggaaaac aaaacagtaa cgaaaaaagt
    64441 cttaaaagaa gctgggttcc gggtaccagg aggcgcagaa ttttcatcca tggaagaagc
    64501 ggtaaaagct tatccgagat ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa
    64561 ttacgggtta ggtatcacga ttttcaagga aggagctagt ctggaagatt atcaagcagg
    64621 tttagccatt gcttttcgtg aagacagttc ggttttagta gaagaattta tgccgggaac
    64681 ggaatatcgt ttctttgtga tcgacggaga agtccaagcc atcatgttgc gagtccctgc
    64741 taatgttatt ggtgacagta tccgaacagt gaaagaactt gttgaagaaa aaaatagtga
    64801 ccctttgcgt ggaacgaatc atcgtgcacc tttagaattg atccaattag gtgaattaga
    64861 acaattgatg ttgaaagaac aagggttgac gatcgaatca gttccccaag ccaatcaaat
    64921 tgtttatcta agggagaact caaatatcag tacaggtggt gattcgatcg atatgactga
    64981 tgaattttct gaagcttaca aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa
    65041 gattagtggt atcgatttga ttataccaga taaagaaatc gatcctacaa ctgataaaaa
    65101 agcatatggg atcatcgaag caaatttcaa tccagccatg catatgcatg tttatccttt
    65161 tgcaggaaaa gacagaagat tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt
    65221 ttaaaagaaa aagcttttga cagattgtcc tatttgatgt caatctgatc aaaagctttt
    65281 tttgcatgta aaaatttatg tgttttttcg ttttaaaata aacgatccca gaagcttttt
    65341 ttcttttttg gtttttcttt ttcagctggc gaagagtttt cttctgggta ttttttttcg
    65401 atatcaatca caggttcatt tactgcttct tttgctgtta agagcaatcc gaattcctca
    65461 ggatttgcag tgacgtaatc gttgactaca gtgaaacggc tattgttttt cgttgctagc
    65521 tggatatatt ggttttggac agaaaggggt agagccccat tcaagagtat ggaagcatct
    65581 ggatgttttt tgagttcttt taaaaagttt tctttgtttt ttggttggat catttcagca
    65641 acagtcatac taagatagca gcgttccctg aatgtcccta aatatttatg ctgttcttca
    65701 gggttcacta aaggtgtacc ataaagtccc ttatccaaat ggctttttac atcatcttgt
    65761 gccataaaaa ataccctcct tcttctcttt gtctttctta cgattatagc atattcccaa
    65821 taatacctaa aacatatgaa aaagcggttg cttttgttca acgattcact taaaataaga
    65881 atgaaaatca ttctcaatta gaaaggtgga ttggatgcgc tacggacgac tagtagcatt
    65941 attagttatt ttaagtagta tttcattgtt catcggagta tcgaatgtac aattgtcaga
    66001 actgctccag ttagatgggc aaagcttaca aatcttgcta gtgactcgtt tgccacgaac
    66061 gatttgtttg atccttgtgg gagcgaccag tagtatttgt gggttgatca tgcaacattt
    66121 gacccaaaat aaatttgttt caccaacgac tgcgggcacg atggatagtg cgcgtttagg
    66181 tattttagtg gctatgattt tcctacctgg tgcatccttg attgtccgat ccttgacagc
    66241 attttgcttt gcttttgcag gaacattgct ttttttatcc cttacgcgat tgttcctcca
    66301 gaaaaatcaa ttgatgcttc cgttgattgg agtcatgtta gggaatatca ttggttcagt
    66361 tgctactttt tttgcttatc aattccagtt ggtacaaaat atgacttcat ggcttcaagg
    66421 gaattttgcg actgtgatac gaggaaatta tgagttattg tatgctgttg ttccattgtt
    66481 gctcttggtt tggctatttg cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc
    66541 agtaaatctg ggaatagatt accagaagat gcagttttta ggcattggct tagtatcatt
    66601 ggctagtgca gtcatgttga tcatggtagg aagcattcct tttatcggag tgatcattcc
    66661 gaatcttgtc tctagaatat atggagatca agtgcacaaa acaattggcg taactgcttt
    66721 agcaggtagt ttgttcttag tattttgcga tcttgtcgct cgcgtgttga tttttcctta
    66781 tgaaatacca gtcagtgtga tcgttggaat catcggtggg gctatcttcc tttatcttct
    66841 gataagggga cgacgatatg cttaaaaaaa agccatctat ttttattggt atgcttgttc
    66901 ttgttttatt tgttacagga atgtacttga cgatccatac atatggaaac tggcattttg
    66961 catgtgcatt gaggagtaag aaaatttttg catttatcct tgtctcgcta gctgtcagca
    67021 gtgccacaat cagctttcag acaattacgc ataaccagtt tttgactcct tcagtattgg
    67081 gacttgagtc gttatatgtc cttgtccaaa cggtcctttt cttctttatc ggaggagtga
    67141 cgatgcttca gcagacaggt acagtttttt tcttattgaa cgttgggctg atggtcgggc
    67201 tgagtctatt gttatttttc tttcttttga aaaaaatgag agcgaatctt tttttattac
    67261 tgatgtcagg gatgatttta ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca
    67321 tcatggatcc gaatgaatat gatttgcttc aaggcagatt atttgcaagc tttggcaata
    67381 tccagagtca gtatcttttg cctgcttctt tgatcattat cggctgctgg tacctgttat
    67441 ttcgatatcg acatgtgcta gacgttttcc atctcggaaa tgaacaagca gtaaacctcg
    67501 ggatagacac agatcgtttt gaatttttga ttttatgttg tgtcagtctt ttgacaggga
    67561 catcaacggc tcttgtgggt ccagttactt ttttagggtt tattgtcgca aatatcagtt
    67621 atcgtgtctt ttctacttat cgtcatggtc agctatttat cggaagcttt ctggtagcta
    67681 tgctgtttct gacaggagga cagctcgcag tagagcagat tttcggctgg aatacaacag
    67741 tgagtgtcgt cgttgaattt gtcggaggaa tttattttgt cacaaaattg attcgcgaaa
    67801 ggaagagtat cctatgattg atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt
    67861 ggttaaagat gtacagttaa cgattcctga aaagaagttg actgctttta tcggtccaaa
    67921 tggtgctgga aagtcaacgg tgttgtcgat gatcagtcgt ttgataccaa aagatacagg
    67981 tgaaatctat ttagaccata acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa
    68041 attatcgatc ctacgacaat cgaatgaaat aaatgtccaa atcaccgtga aagaattagt
    68101 agaatttgga cgatttcctt atacgaaagg aaaattgacg acaaaagacg aggaaatcgt
    68161 tcaggaatca ctggaacacc ttgggttaga agaaatagcg gatcaatcga ttgacactct
    68221 ttcaggcggt caactccaac gtgcgtatat tgcaatggtg ctagcgcaag acacggatta
    68281 tattttactg gatgaacctt tgaacaatct agatatgaat tttgcggttc aaatcatgca
    68341 gattttaaaa aatttagtac aagaaatcgg gaaaacaatt gttattgttc ttcatgatat
    68401 caactttgct gcaagttatg cagacgaaat tgttgcaatg aaagatggac agttatttac
    68461 gcaaggaacg acccaagaaa tgataaaaaa agatgtattg gatgaattat acgaaatgga
    68521 catacggatt tgtgagattg aaggccgcag attctgtctt tatttctaaa actaacatta
    68581 ggagagaaga gatgaaaaaa ttagtagtag gtatcttgat tctagcatca tttggtttag
    68641 cagcatgtgg aaatagcaac aacaccagtc aggcagacac aaaaaaaagc agcacacaaa
    68701 ctgaaactac attgacgata acagatagta atggagacca aatcgaggtg ccaaataatc
    68761 cagaaaaagt cgtcgtattc gacaatggtt ctttagatac gatggatgct cttggtgttg
    68821 gagataaggt agttggagca gctacaagca gccttcctga gtatctttca tcctataaaa
    68881 aagtagaatc agcaggtgga ataaaagaac cagacttaga aaaaattaat cagcttcagc
    68941 cagatctaat catcatttct ggccgacaaa gagattttca aaaagatcta agtcaaattg
    69001 ctccgaccat tttcctttct ttagatgcga aaaatccatg ggaatctttc cagcaaaatg
    69061 tcactgcttt aggggagatc tttggtaagc aagaagaagc taaaactcaa ttagaggaat
    69121 tatctagtgc aatcgatcag acgaaaaaga aagcagaagc tactgacaaa aaagcactag
    69181 tcacacttgt gaatgaaggg caattatcag cctacggatc tggttctaga ttcggtttca
    69241 tccatgattt atttggcttt gaacaagcag atgatcaaat cgaagcatcc actcacggtc
    69301 aaagtgtttc ttatgaatat gtgctggaaa aaaatccgga tatcttattt gtagtagaca
    69361 gaacaaaagc tattggtgga gacgacagca aagatgatat ttctgccaat gaactggtag
    69421 cacaaacgaa tgctggtaaa aatcagcaga tcatctccct tgaaccagat gtctggtatt
    69481 taagcggggg cggtttagaa tcaatgaaac tgatgatcga agatgtgaat caagcattta
    69541 aatagcataa ataacagagg attaaactct catactatgt aacaataatg tttcttacat
    69601 tcttaaatag caagagccta tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat
    69661 tgatgatttt acggatggtt tttatttatt tgagaaatct tttttcaacg tgaattcatt
    69721 atctacatca tacgggttga ttctagaatg gggagtggca aaaaatagtg tgatccattt
    69781 tttcagcatc aagatcgcct cctttgattt gaatcaaagg tacaaagcga aacttagata
    69841 aaacttaaat ttagatattt ttattttgta ccgatctctt ctttaaacga tgttccatct
    69901 gctgcttctc ggcaataagt ccactagact agaaaatatg agaagctatt ttctgtccag
    69961 cgttcttatt actcaaaaca aatcgcagtt gtcacaatct cttctaaacg atgttccatc
    70021 tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat tttctgtcca
    70081 gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat
    70141 cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt aggcatgtgt
    70201 ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg cgggaagctt
    70261 actccaccgt tttagtaatt gatagaaata actctcttct gctttgattt tatactcgct
    70321 taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa tatccaatgc
    70381 tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac cctcgatatt
    70441 ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga ttcctccaaa
    70501 gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc gtgaacaaaa
    70561 agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat tttttgttat
    70621 ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt ttccgttcaa
    70681 gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat cataataaac
    70741 gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta gttgcatctg
    70801 atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga agtgacaaaa
    70861 gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt attagatatc
    70921 gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga tacggcattt
    70981 cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc tgtagtggct
    71041 gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat ggatgatcca
    71101 tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc aagcggcttg
    71161 agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg aaaattggcg
    71221 atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct gatgcagctt
    71281 tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga tacggctaat
    71341 aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt atctacgaca
    71401 tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat ggaccatttt
    71461 ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac cgttctttta
    71521 gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga ggcaatcgta
    71581 cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac agatctcgag
    71641 gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga agcaaaaaaa
    71701 cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat gtatcaatct
    71761 gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc attcggagaa
    71821 ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt
    71881 gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta ttattatgaa
    71941 tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta tgatcctatg
    72001 aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca aacacaacat
    72061 ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag cattagtaat
    72121 gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga tccatttatc
    72181 gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga tcggatgatt
    72241 tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat aatcacaaaa
    72301 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
    72361 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
    72421 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
    72481 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
    72541 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
    72601 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
    72661 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
    72721 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
    72781 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
    72841 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
    72901 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
    72961 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
    73021 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
    73081 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
    73141 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
    73201 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
    73261 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
    73321 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
    73381 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
    73441 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
    73501 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
    73561 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
    73621 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
    73681 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
    73741 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
    73801 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
    73861 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
    73921 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
    73981 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
    74041 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
    74101 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
    74161 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
    74221 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
    74281 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
    74341 caaagaagtc ttacttatga tttggaaggt gtcggtcgct cttctcggat tatacctgat
    74401 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
    74461 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
    74521 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
    74581 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
    74641 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
    74701 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
    74761 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
    74821 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
    74881 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
    74941 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
    75001 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
    75061 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
    75121 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
    75181 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
    75241 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
    75301 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
    75361 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
    75421 acagctcatc gtggaatcag aataaatatt cctgtagaaa accaaagaat cggaaccttt
    75481 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
    75541 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
    75601 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
    75661 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
    75721 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
    75781 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
    75841 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
    75901 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
    75961 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
    76021 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
    76081 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
    76141 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
    76201 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
    76261 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
    76321 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
    76381 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
    76441 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
    76501 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
    76561 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
    76621 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttatcg tttattttat
    76681 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
    76741 acaaatagta aatttttatt attgtaggat ggttgggctc tttttataac aatataaaat
    76801 ataccatttt attaataaaa atgcatacta gtaaatgata taattctaat aacaggtttg
    76861 atcgcgagaa aggccaaata aaacgctctc caaaatgtta aaaaaaagac aggacgatgt
    76921 atattataca aacaagaatc gctgacaaca gtgactcgga tccaggaaat cagttttatc
    76981 cccagctagt tggtgaatgc atggaaagta atatacgtag aaagagctga tgactaatga
    77041 attatgagtt ttttttaagc aaagaagatc acagaagtta ttcattactt aaatatttag
    77101 aagcaagctc aaatttatca gagtcaatat cgaatgttca agaagagttg tcgctatcga
    77161 catttctctt gaaaaaaacg atcgataagc tgaaacatga tttacaaaag ttcgatctag
    77221 agaaaaattt gcgagtaacc gtcagtgaat tggatatatg tctggaaatt aatggaaaat
    77281 gttctagcaa agctctgtta tctcgttata taacggattc tttatctatg aaaatggtat
    77341 tggctttctt tcaggaaaaa tatgtctcag taga
//
LOCUS       DACCIQ010000004        75707 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCIQ010000004 DACCIQ010000000
VERSION     DACCIQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 75707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.004"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(17..496)
                     /gene="rlmH"
                     /locus_tag="IVM62_000252"
     CDS             complement(17..496)
                     /gene="rlmH"
                     /locus_tag="IVM62_000252"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6844247.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            924..2213
                     /locus_tag="IVM62_000253"
     CDS             924..2213
                     /locus_tag="IVM62_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6844248.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2252..2857)
                     /locus_tag="IVM62_000254"
     CDS             complement(2252..2857)
                     /locus_tag="IVM62_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6844249.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3062..3526)
                     /locus_tag="IVM62_000255"
     CDS             complement(3062..3526)
                     /locus_tag="IVM62_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6844250.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3548..3868)
                     /locus_tag="IVM62_000256"
     CDS             complement(3548..3868)
                     /locus_tag="IVM62_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6844251.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4012..5091)
                     /gene="pepA"
                     /locus_tag="IVM62_000257"
     CDS             complement(4012..5091)
                     /gene="pepA"
                     /locus_tag="IVM62_000257"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6844252.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5291..5641
                     /locus_tag="IVM62_000258"
     CDS             5291..5641
                     /locus_tag="IVM62_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6844253.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5864..6811
                     /locus_tag="IVM62_000259"
     CDS             5864..6811
                     /locus_tag="IVM62_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6844254.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(6868..8286)
                     /gene="pepV"
                     /locus_tag="IVM62_000260"
     CDS             complement(6868..8286)
                     /gene="pepV"
                     /locus_tag="IVM62_000260"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP6844255.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8435..9280
                     /locus_tag="IVM62_000261"
     CDS             8435..9280
                     /locus_tag="IVM62_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP6844256.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9284..9724
                     /locus_tag="IVM62_000262"
     CDS             9284..9724
                     /locus_tag="IVM62_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP6844257.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            9774..9968
                     /locus_tag="IVM62_000263"
     CDS             9774..9968
                     /locus_tag="IVM62_000263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844258.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10104..10790
                     /locus_tag="IVM62_000264"
     CDS             10104..10790
                     /locus_tag="IVM62_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6844259.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(11198..11887)
                     /locus_tag="IVM62_000265"
     CDS             complement(11198..11887)
                     /locus_tag="IVM62_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844260.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(11862..13991)
                     /locus_tag="IVM62_000266"
     CDS             complement(11862..13991)
                     /locus_tag="IVM62_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP6844261.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14039..15376)
                     /locus_tag="IVM62_000267"
     CDS             complement(14039..15376)
                     /locus_tag="IVM62_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6844262.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15628..15987)
                     /gene="rplT"
                     /locus_tag="IVM62_000268"
     CDS             complement(15628..15987)
                     /gene="rplT"
                     /locus_tag="IVM62_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP6844263.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16040..16240)
                     /gene="rpmI"
                     /locus_tag="IVM62_000269"
     CDS             complement(16040..16240)
                     /gene="rpmI"
                     /locus_tag="IVM62_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP6844264.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16312..16836)
                     /gene="infC"
                     /locus_tag="IVM62_000270"
     CDS             complement(16312..16836)
                     /gene="infC"
                     /locus_tag="IVM62_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP6844265.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            17156..17788
                     /locus_tag="IVM62_000271"
     CDS             17156..17788
                     /locus_tag="IVM62_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6844266.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(17790..18695)
                     /locus_tag="IVM62_000272"
     CDS             complement(17790..18695)
                     /locus_tag="IVM62_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6844267.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(18780..19433)
                     /locus_tag="IVM62_000273"
     CDS             complement(18780..19433)
                     /locus_tag="IVM62_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6844268.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            19755..20699
                     /gene="mvk"
                     /locus_tag="IVM62_000274"
     CDS             19755..20699
                     /gene="mvk"
                     /locus_tag="IVM62_000274"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP6844269.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            20692..21669
                     /gene="mvaD"
                     /locus_tag="IVM62_000275"
     CDS             20692..21669
                     /gene="mvaD"
                     /locus_tag="IVM62_000275"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP6844270.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            21682..22767
                     /locus_tag="IVM62_000276"
     CDS             21682..22767
                     /locus_tag="IVM62_000276"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP6844271.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            22764..23828
                     /locus_tag="IVM62_000277"
     CDS             22764..23828
                     /locus_tag="IVM62_000277"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP6844272.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24077..24880)
                     /locus_tag="IVM62_000278"
     CDS             complement(24077..24880)
                     /locus_tag="IVM62_000278"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP6844273.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(24900..25742)
                     /gene="kduI"
                     /locus_tag="IVM62_000279"
     CDS             complement(24900..25742)
                     /gene="kduI"
                     /locus_tag="IVM62_000279"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP6844274.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(25758..26765)
                     /locus_tag="IVM62_000280"
     CDS             complement(25758..26765)
                     /locus_tag="IVM62_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP6844275.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(26772..27416)
                     /locus_tag="IVM62_000281"
     CDS             complement(26772..27416)
                     /locus_tag="IVM62_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6844276.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27516..28274)
                     /locus_tag="IVM62_000282"
     CDS             complement(27516..28274)
                     /locus_tag="IVM62_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP6844277.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28401..29552)
                     /gene="fucO"
                     /locus_tag="IVM62_000283"
     CDS             complement(28401..29552)
                     /gene="fucO"
                     /locus_tag="IVM62_000283"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP6844278.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(29634..29945)
                     /gene="rhaM"
                     /locus_tag="IVM62_000284"
     CDS             complement(29634..29945)
                     /gene="rhaM"
                     /locus_tag="IVM62_000284"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP6844279.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(29947..30792)
                     /gene="rhaD"
                     /locus_tag="IVM62_000285"
     CDS             complement(29947..30792)
                     /gene="rhaD"
                     /locus_tag="IVM62_000285"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP6844280.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(30806..32062)
                     /gene="rhaA"
                     /locus_tag="IVM62_000286"
     CDS             complement(30806..32062)
                     /gene="rhaA"
                     /locus_tag="IVM62_000286"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP6844281.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32059..33519)
                     /gene="rhaB"
                     /locus_tag="IVM62_000287"
     CDS             complement(32059..33519)
                     /gene="rhaB"
                     /locus_tag="IVM62_000287"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP6844282.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            33672..34541
                     /locus_tag="IVM62_000288"
     CDS             33672..34541
                     /locus_tag="IVM62_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6844283.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(34667..35566)
                     /locus_tag="IVM62_000289"
     CDS             complement(34667..35566)
                     /locus_tag="IVM62_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6844284.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(35731..36555)
                     /locus_tag="IVM62_000290"
     CDS             complement(35731..36555)
                     /locus_tag="IVM62_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6844285.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(36566..37861)
                     /locus_tag="IVM62_000291"
     CDS             complement(36566..37861)
                     /locus_tag="IVM62_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP6844286.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(37877..38356)
                     /locus_tag="IVM62_000292"
     CDS             complement(37877..38356)
                     /locus_tag="IVM62_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP6844287.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38362..39390)
                     /locus_tag="IVM62_000293"
     CDS             complement(38362..39390)
                     /locus_tag="IVM62_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP6844288.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39407..40513)
                     /locus_tag="IVM62_000294"
     CDS             complement(39407..40513)
                     /locus_tag="IVM62_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6844289.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(40742..43339)
                     /gene="adhE"
                     /locus_tag="IVM62_000295"
                     /gene_synonym="adhC"
     CDS             complement(40742..43339)
                     /gene="adhE"
                     /locus_tag="IVM62_000295"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP6844290.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(43644..44024)
                     /gene="yajC"
                     /locus_tag="IVM62_000296"
     CDS             complement(43644..44024)
                     /gene="yajC"
                     /locus_tag="IVM62_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP6844291.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44121..45266)
                     /gene="tgt"
                     /locus_tag="IVM62_000297"
     CDS             complement(44121..45266)
                     /gene="tgt"
                     /locus_tag="IVM62_000297"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP6844292.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            45484..46182
                     /locus_tag="IVM62_000298"
     CDS             45484..46182
                     /locus_tag="IVM62_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6844293.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46350..48989)
                     /locus_tag="IVM62_000299"
     CDS             complement(46350..48989)
                     /locus_tag="IVM62_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6844294.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(49608..50639)
                     /gene="queA"
                     /locus_tag="IVM62_000300"
     CDS             complement(49608..50639)
                     /gene="queA"
                     /locus_tag="IVM62_000300"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP6844295.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            50832..52007
                     /locus_tag="IVM62_000301"
     CDS             50832..52007
                     /locus_tag="IVM62_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6844296.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52011..52652
                     /locus_tag="IVM62_000302"
     CDS             52011..52652
                     /locus_tag="IVM62_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844297.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            52649..53566
                     /locus_tag="IVM62_000303"
     CDS             52649..53566
                     /locus_tag="IVM62_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6844298.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            53563..54234
                     /locus_tag="IVM62_000304"
     CDS             53563..54234
                     /locus_tag="IVM62_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844299.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(54775..55263)
                     /locus_tag="IVM62_000305"
     CDS             complement(54775..55263)
                     /locus_tag="IVM62_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP6844300.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(55464..55730)
                     /locus_tag="IVM62_000306"
     CDS             complement(55464..55730)
                     /locus_tag="IVM62_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6844301.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(55730..55993)
                     /locus_tag="IVM62_000307"
     CDS             complement(55730..55993)
                     /locus_tag="IVM62_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6844302.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(55986..56258)
                     /locus_tag="IVM62_000308"
     CDS             complement(55986..56258)
                     /locus_tag="IVM62_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844303.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            56469..56654
                     /locus_tag="IVM62_000309"
     CDS             56469..56654
                     /locus_tag="IVM62_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6844304.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(56796..58619)
                     /locus_tag="IVM62_000310"
     CDS             complement(56796..58619)
                     /locus_tag="IVM62_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP6844305.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59217..61922)
                     /locus_tag="IVM62_000311"
     CDS             complement(59217..61922)
                     /locus_tag="IVM62_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP6844306.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62228..63175)
                     /locus_tag="IVM62_000312"
     CDS             complement(62228..63175)
                     /locus_tag="IVM62_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP6844307.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63365..63634
                     /gene="rpsN"
                     /locus_tag="IVM62_000313"
     CDS             63365..63634
                     /gene="rpsN"
                     /locus_tag="IVM62_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6844308.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(63810..64172)
                     /locus_tag="IVM62_000314"
     CDS             complement(63810..64172)
                     /locus_tag="IVM62_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6844309.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64183..65304)
                     /locus_tag="IVM62_000315"
     CDS             complement(64183..65304)
                     /locus_tag="IVM62_000315"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP6844310.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65320..65670)
                     /locus_tag="IVM62_000316"
     CDS             complement(65320..65670)
                     /locus_tag="IVM62_000316"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP6844311.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(65800..67311)
                     /locus_tag="IVM62_000317"
     CDS             complement(65800..67311)
                     /locus_tag="IVM62_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6844312.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(67633..68985)
                     /locus_tag="IVM62_000318"
     CDS             complement(67633..68985)
                     /locus_tag="IVM62_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP6844313.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69138..70214)
                     /locus_tag="IVM62_000319"
     CDS             complement(69138..70214)
                     /locus_tag="IVM62_000319"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP6844314.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70410..72155
                     /locus_tag="IVM62_000320"
     CDS             70410..72155
                     /locus_tag="IVM62_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP6844315.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(72435..72872)
                     /locus_tag="IVM62_000321"
     CDS             complement(72435..72872)
                     /locus_tag="IVM62_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP6844316.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(72856..74229)
                     /locus_tag="IVM62_000322"
     CDS             complement(72856..74229)
                     /locus_tag="IVM62_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844317.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(74551..75039)
                     /locus_tag="IVM62_000323"
     CDS             complement(74551..75039)
                     /locus_tag="IVM62_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844318.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75061..75663)
                     /locus_tag="IVM62_000324"
     CDS             complement(75061..75663)
                     /locus_tag="IVM62_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6844319.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21697 a        16202 c        13885 g        23923 t
ORIGIN      
        1 ctcttaaaaa atatcattat ttgtggtatg gagcccccgc attgatccgg aaggcacggt
       61 agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt tttccaaaag
      121 agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag ccgccgataa
      181 caaaaacaag gtggcttttt ccttggatac ctaattggtc gatttgttta gcaaaagatt
      241 cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt tctttgattt
      301 tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct tcgcttaaat
      361 tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag gcattcagcc
      421 gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt cctactgaga
      481 taattttgat attcatgttt tctctccttt ggtcttcatg ctcactttgt ttattataat
      541 caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt ccccaacagt
      601 tatgcacaac atccacaggt ttatccacag aaaacagtgt tattttacct tatttcatca
      661 cattcattag gcaaacatac gttttataag aatttatcca ctttatacac aggctgtgga
      721 tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt atctgtggaa
      781 aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt ttcataaaaa
      841 gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca gtagataaat
      901 gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag atgaaaaaaa
      961 gtaaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg atcgttggag
     1021 gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat agttctactg
     1081 ctacaagcgg caatcagaat tctgccggtg aaacagttgt ggaaaatgtc aaagtcaatg
     1141 tagattctga tatcaccaaa gcggtagata aagttcaaga cgctgtcgtt tctgttatca
     1201 acttacaaag tcaaaaccaa tcaagcggtt ttggcggact attcgggcag caagaagaaa
     1261 gtagcagctc agatgatagt aatttggaag cttatagtga aggaagcggc gtgatctaca
     1321 aaaaagacgg gaatactgct tatgtagtca cgaataatca cgtagtagat ggccagcaag
     1381 gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc tgaattagtt ggaacagatg
     1441 cttattcaga tttagcggtg ttgaagatca attcagacaa agtcgaaaca gttgcctctt
     1501 ttggtgactc aagtgctctg aaggttggtg agcctgcgat tgcgattggt tccccactag
     1561 gatctgaata tgcaaattct gtcacttcag gaatcatttc ttccttgaac cgacaagtga
     1621 caagtacgaa tgaatcgaac gaaacagtca atatcaatgc gatccaaaca gatgcagcaa
     1681 tcaaccctgg aaattctggt ggtcctttag ttaatatcga aggacaagtt atcggtatca
     1741 actcaagtaa gatcgcaagt acttctgaat ctacttctaa tgtaagcgta gaaggaatgg
     1801 gatttgctat tccaagtaac gatgtagtga atatcatcaa tcaactggaa aaagacggaa
     1861 aagtgacacg tccagctcta ggtatcacaa tggtcgactt atctgccgtt tctactcagc
     1921 aacaagaaca aatcttgaag attccttcat ctgtaactaa cggtgtcatc gtgacaaatg
     1981 tccaaacagc tacaccggct gaaaaagctg gattgaaaca atacgacgtc attactaaga
     2041 tcgatgacaa agaagtttct tctggtgtag aacttcaatc tgctttgtat aagaaaaaag
     2101 tgggagattc tgtcaaaatc acttactatg aaggctcgaa agaaaaaaca actactgttg
     2161 aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa tagtgaaaat taataaaaaa
     2221 gaggttgtga caaagacttg tcacaacctc tttattttgg aaatgcttct ccgactacgg
     2281 catcctcagg caatactaag attccttttt ttgctggagc atttggcaaa tgcaattcac
     2341 gagcggaaca gatcattcca tagctatcta cccctcgcaa agttcctggc cagatcatca
     2401 ttccatctgg catcatagct cctggttttg caacgacaac tttttgtcca gcttcgatat
     2461 tgggtgcacc gcaaacgatt tgcaatactg taccattgtc tacttctgtc tcagtaatcg
     2521 ataaatggtc agaatcagga tgtgggacgc atgtttttac gtacccgaca acgatcttcg
     2581 gttcatgatc agcttcaagc tgttcttcga atcctgcttc tttgatcaat cgattcaatt
     2641 tcgctaattc ctcatctgtt aagcttactt ggccgttccc ttcgattgtt agatgatcgg
     2701 agatatggaa aaagttccaa gcaacaactt gaccttgttc atctttgatc cgagcaacat
     2761 tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc ggctataata accatcaagg
     2821 tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt ctttcctcca ttatcctttg
     2881 ctgagattta tcttaacaaa aaattgcatt cactgcatga aatttttttc atgctttatt
     2941 tccgaagaag cctcttctgg aacaccattt attcgtaaca agggactgtg acaagacttt
     3001 tgtcacagtc cctttactgt tttagttaat tgattaaaaa ggtcgtacaa gttccacttg
     3061 cttaattact tggctgttct tcttcgtcag aaacgatcaa gacatcacaa ggtgaatgac
     3121 gtatcacgta gctggctaca cttcctgtca taaagcgttc gactgcattt agacctgact
     3181 gcccaaccat gattagatcg atctcatatt tttttggcaa tttgactgcc atggattcac
     3241 gaacagaacc atatgcaata attccttcta tatttgtgta atcaacagat ttcccatatt
     3301 ctttacattc ttcgatcatt tccttggctg tttcagtttg ctgatcgact agattttgat
     3361 caataggtgc atagcccata aagttataaa attgctgatc gattacgttt gctacatata
     3421 ctttcccatt attacggcga gccacttcta ccgctttttt gaaagcagca agtgcttgtt
     3481 cactaccgtc gatacctact aaaatattct tatattcttg attcatcttg acccctccta
     3541 acagatgtca gccttttgtt acactttcta ggaacgattc gatttcctgt tttgttttac
     3601 gatctttatt cactagacga cctaattctt ttccgtcttg gatcacgaca aaacttggga
     3661 taccaaaaat gttccattca gcggctacat ctatgtactt gtcacggtct acttcaataa
     3721 attgccaatc agaaaagtca ttttcaattt caggcatttg cggtttgata aaacggcagt
     3781 ctccgcacca gccggcagta aagaaaaata cactctttcc agtttctacg tagcctgcta
     3841 attcttctaa actatttggg ataatcattc tttgtcctct actttctgtt tatgtatttt
     3901 tcaattaaat tatacacctt tctttcgaac taacaaacat tgatcctctt tttataaaaa
     3961 aaatctggca aaaagcaatc ttttgccaga ttttatagtt tcactatcgc attattttcc
     4021 gtatacgatc gtatttactg ttgatttatc caaacctttc agtgtttgaa tcaacatttc
     4081 tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga tgcgtatgga tataacgtcc
     4141 cactacgcca atcactgtac ttgggattcc ttcgttttgt gtatgagcag cacctgcatc
     4201 tgtaccgcct tttgaaacaa agtattgata agggatattg tttgtttcag cgatatccag
     4261 caagtactcg cgcaaacgag gaagcatgat caaacctgga tcttggatac gcatcaatgt
     4321 accttctcct aaatgtccga atgtgccatt tttcgtcact gtatcatctg ctgcagagca
     4381 gtctactgca aagaacagat ctggcttgaa tttattgaca gaagctttag aaccacgcaa
     4441 gcctacttct tcctggacat tcgcgcctgc aatcaatgta tgacctaatg tttcattttg
     4501 caacgcttct aaagcttcta ataccattgt acagccatag cggttgtccc atgatttact
     4561 gataatattc tttccgttgg ctgttttgat cgtttcagcg taaggaacga ttgtgtctcc
     4621 tggaaggaca ccgtaactca ttgcctcttc tcttgattca aacccagcat cgaacaaaat
     4681 atcggttact tctactgatt tttgtccgct tgttcctcgc aataaatgtg ggggaacgga
     4741 agaagaaata catggataat tcttaccagt agaggtttta agtgtaaaac gctgagcaga
     4801 aacaacgtaa ggattccagc cgcctagagg tacgacttta aaaagcccgt tttcttggat
     4861 ttgtgtcagc atgaatccga cttcatccat atgtgctgcg accattacac ggggtgcatc
     4921 cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa ccatctaact gtaactcatc
     4981 taccagcggt tccatatgac tttccatata agcgcgaaca tcttgttcaa aaccgcttgt
     5041 tccttgtaat tctgttaact ctttgattcg ttgaaatgtt ttttcttcca tatgctattc
     5101 gtctaaattt cagactaaac tcctctcaga cttatattga tctgcggata aaatatgatt
     5161 ttttctgcag accgatcgta tttattatag cttatttttt ctggtagcgg ttatttattt
     5221 caaagaaaat atgagagtgt ggtaaaataa gcacaaggag taacaagtga gaagaaggag
     5281 gtatgtccac atgtatgaag aaagcgaact aagctatttt aaaggcgggt tagcagttgg
     5341 aatcggtcta ggattattga gcggggtagc ttcaacgatt tggtatcatc gcaaaaaagt
     5401 attgtcagca gaccaagtac ttgaaaatat caagtcatct tttctaaaag aaggtgcaat
     5461 cgaaggttct tggatctctt ttgaaaagga acctattagg aaattcgccg ttcggtcgag
     5521 tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa actgtttatt atgaattttt
     5581 agcagatgct catacaggaa ccgtattaga aatttcaaga aaaaaaggtg caacagatta
     5641 atacgtcaaa aaagtgagag aacgaatacg cacccactat tcaaaagaga ggtatcatgt
     5701 ccttcttttg tagtgggtgg ttttttagat agagctttcc aaacatatat tccagtattt
     5761 tctttttaaa taaatgatat ttagcgggaa aaactagcat ttcctttgat tatgaggtat
     5821 ttttaattta gcttttgttt tctatagtta aggagagtga aaaatggaac aacaagaaaa
     5881 aattgctgtt ctgattcctt gttacaatga ggaagcaaca atttcaacag tcattgcaga
     5941 ctttaaacga gaattaccag aagcagatat ttatgtctat gataataatt cgaccgatcg
     6001 aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa aaagaacctc gtcaaggaaa
     6061 aggaaatgtg atccgccaaa tgttctttga tatcgatgcc gactattatt taatggtaga
     6121 tggcgatgat acttatcctg cagaagctgt acatggtttg ctcgagaaat tacgttctgg
     6181 tgaagcagat atggtcattg gtgatcgcct atcaaatgga acctactttg atgagaataa
     6241 gagacctttc cacgattttg gaaataacct tgttagaaat acaattacta gaatgtataa
     6301 aacaaaaatt cttgatgtca tgaccggcta tcgaggattc aatcgtatct ttgtcaaaag
     6361 ttttcctatt atgagttctg gttttcaaat cgaaacggaa ttgacgatcc atgctttaga
     6421 taaaaaattc aagttcgttg aaatcccaat cgattatcgt gatcgtcctg aaggcagcga
     6481 atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatta ttaaaatgtt
     6541 taaagattat aagcctttgc ttttctttgg gatttggacc atcttcttct ttttatttgg
     6601 attggtcacg gggattcctg tgattcgcga atatatgctg acaagcttta ttacacgcat
     6661 cccttctgcg attttaagta ctggattgat gactttagca cttctttcat tagtaacagg
     6721 gttgattttg gatacagtcg tcacgaacgc taagaaagaa tatgaattga atctatatca
     6781 tgtttatcat gaataccaca gtagaaagta aaaaaaggat caagactgca aaaataattt
     6841 gcaattcttg atcctctttt taattaatta tttcgccaaa cgatagatgg catctgcata
     6901 gattgctgtt gctctgaaca agtcatcaag actcataaat tcgtttgctt gatgcattgt
     6961 atcagtatat cctgggaaca tcgctccata agcaacgcca cgtttcaaca aacgtccgta
     7021 agttccgcca ccgatgattt gttcgtagcc tttttctcca gtgtggtctt catagacttg
     7081 caataatgta gccaccaatg gatcatccat tggtacgtaa tgtggttcca ttacgcgagc
     7141 acctcgagta actgttgcgc cttcttgccc cactgtttgt tcgatgtcac tttgcaatcc
     7201 gtctactgtt acaccttttg gaaaacggaa attcatattg atgaagttcg cttcctctgt
     7261 accattttct tcaaaagcaa atagtccagc attcattgtc aatttgccca ttttttcatc
     7321 ttcataagcg actcctagtt tttctccata aaaatcttca tggacataag cagctgctac
     7381 atggataaat tgtttagcag atcctaggaa ttcatagtta tctaagaaca atgccaagaa
     7441 agaacctgca ttgataccag attgtgggct tgcaccatgt gcgcctttac caactaattc
     7501 gatttttaca tacgtattgt ccgcttcaat cgttccgctt actggatttg cttcgatgaa
     7561 ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca cttggtactt gaagtgctgc
     7621 agttgctgtt cctggcacca tattttcgcg aagtccggaa acaaaagatt tcaagacata
     7681 atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa acatttcctt tttcaccatt
     7741 gatgattggg aattcagcat ctggtgagaa accaaaatct ggttcttctt catgttcgaa
     7801 ataataagcc atatcgcccc agccgctttc ttcatcgctg ccgactacga agcgaacttt
     7861 ttttgatagt ttcaagtcaa gttctttaat gatcttcatc gcatagtaag ctgccatact
     7921 tggacctttg tcatcgcttg atccgcgtgc aaagatcttt ccatccttga ttactggttc
     7981 ataaggatct gtgtcccagc catctcctgc aggaacgaca tccatgtgtc caaatatccc
     8041 tagcgtttcg tctccttcgc caagatcaat atgccctgca tagttatcga cattttttac
     8101 tttaaatcca tcacgttcgc cataagctaa catatgtttc aatgcgtcgc gaggtccagg
     8161 tccaaatggt gcatctggtg ttacttttga gtcatcgcgc tcgctgttga cacgtaaaag
     8221 atttttcaag tcttcaagca agtcttcttt acgtgcttct acttcttttt gccaatcaat
     8281 tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt tcatgatacc acaaattaca
     8341 gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta tctgtttttc tctaaaaaaa
     8401 atatacgcta taatgagtaa aaggaggttt ttagatgctg ataaacgaac aactagtgaa
     8461 aagagtcatc acacctcgtc caaaaatttc acataaagga acatttggac gtatcgtttt
     8521 gattggcggc aatgaacaat atggcggagc aatcattatg agtgctttag ctgccgtaca
     8581 cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa aatcggagtg ctcttcacag
     8641 tcatctgcca gaagcaatga ttgtagtttg gacggatact gaccagtttc tttccgtaat
     8701 aaagcaagca gatgtactct tgattggtcc cggattgggt acttcttcta agagtttgga
     8761 acgtttcaaa caagtactag aaaaccaatc aaaagaacaa tggctggtga ttgatggctc
     8821 tgctttaagt ttacttgctg ctaatcatct tttgctaccc tatcccgaac aaactgtact
     8881 tacacctcat caaatggagt ggcaacggtt gagttctcta ccgatacaag aacaacggga
     8941 taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg ctcgttttga aaagtcatga
     9001 gacaaaaatt tatggtcacc agcacttcta caccaatcct ttaggttctg ctgctatggc
     9061 aacgggcggc atgggtgaca ctttagctgg gatgatcaca ggatttttag cccagtttcc
     9121 tcaaaatgac caaacgattg cagcagctat ttatcttcat agtttcattg gagaccagct
     9181 ggcaaaagaa caatatattg ttttgccgac acaaatcagc gaagcacttc cgaaatggat
     9241 gaattatttt tgtaaaaaat caatggaaaa cgaggcatga gtcatggaga aatttacagg
     9301 ttatgtgcga gaaccttttt attatgaaac agatcaaatg gggattattc atcacagcaa
     9361 ttatatccgc tggtttgaag aggctcgggt agccttattg gtacatctag ggtttcccta
     9421 taaaaaaatc gaagaatctg ggatcatcat ccccgttctg gaagtaaatt gcagctataa
     9481 agaaatgatc cattacggag aaaaagtgag catccatcca actatccaaa agtatacggg
     9541 gacacggttg gattttagct acgaaattgt gggaactgaa gacaaaaaat taaaaactac
     9601 tggcagtagt aaacactgtt ttttactttc tgattcgcaa cgattagtga agctgagcaa
     9661 agtcaatccg gagttagatc agttattcca agcgtacgct tctttggatg atgtaaattc
     9721 ttaataaaga ccgtctaagc atttttcttg tattgtgtcg agtagttcgt ataatgaaaa
     9781 gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat gcacggttac atcgataaaa
     9841 ctggctgtcg cactcgggaa aaaaacttag acaatctgct cattttcaaa gacaaaaaag
     9901 aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa accgatccaa gaggtacgac
     9961 cagattgatt caatattttt cttttcgagg ctctaatagg catgcctatc agggcttttt
    10021 ttgcagactg tgtttttatg cccacacgat ttgtaagcga tttatgttat aatagattat
    10081 atctgcatag aaaggaaggt taaatggtta tcaataattt gcaacagcaa gcttatgaat
    10141 ctatacgtaa acaaatcatt tattccgagt tagcacctgg aagtaaaatc tccgataaag
    10201 tgttagaaga acaactagct atcaggcgta caccgattcg agaagcactg atccaactgc
    10261 gcaatcaaga gcttgtaaaa accattccac agtccggcac ttatgtttca aaaatcgata
    10321 tccgatctgc ttcattagcc cgttatacgc gggaaaagtt ggaaaaaccg atcctgcaag
    10381 aatgcagtgc gaaaatgacc aagcaagatc aagctaaact agaaaaaata ctagaacaaa
    10441 ccgatcaggc agtaatcgct caaaataaaa aagctttttt tgatttggat actgattttc
    10501 accgaacttg ttatgaaatt gctggaaaac gagaaatttg ggattggcta gaaagttata
    10561 gtacgcactt gaaccgtttc agatggttac gtttgacgat ttcagaatta gactggggtc
    10621 gagtattaga tgaacatcag acaatgcttc aatcaatgat tgatcataac tttgatgaag
    10681 tcggatttct ttgcacgatg catttacata tgattatcga ggagcaagaa tacgtgattc
    10741 ataattaccc cgattatttt gaagatattc aggacagacc catcaaataa gaggttttgg
    10801 aatcagcgtt ttgacctgag tattagagaa agaaacggca aaatagttcc tcatattttg
    10861 ccgttttttt tgagctaata ccgagggtca gctgattgca caccgtttat tgagaggttg
    10921 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaatagc ttcttatgtt
    10981 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt ttccaagagg
    11041 tcgtgagcct gccgttcatc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat
    11101 gtttgttgaa tattttgact tgtgaccgag gagttgacag gagaacaccg tttattcgga
    11161 aaaaaaggac tgtgacacga cttttgtcac agtctcttta tttgtaatct aaatcatact
    11221 ccgtacatct tttttctatt ttttttattt cttctaagat cccttggcta cgaatttcat
    11281 tatatggcca aagtaagact gcctctaata cataaggcga aaaatcgctt tgattcatct
    11341 cttttgaaaa actcatcgct gctatctcgc tcaatgcacg aatgtttgat tgatgtgtat
    11401 aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt ataaattccc ttaacagaag
    11461 cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc ttgcgctgtt tctttagaga
    11521 tcgaagctag tcctgaacat tttaattttt ctaacggctc cgtcatcaat cgaatccctt
    11581 catcttctgc ataagttgca aaagtgattc gttcgtttat ttgctctgat tcacaggaaa
    11641 cgattggaac agccatttgt tcacaaattt ctcctacatc ggcaaaaggg tacttttttt
    11701 tgatgctaca tgccgtttcg ataccacaat cgattgattg ttgtataaga attttctgct
    11761 tggacaaagg aatctttttc caaaataaat ctcgccgaaa ctggtagaat ccccaaattt
    11821 cttcggggat ctgttgtaat tcgtttagta actcttctaa cttatgtttg tttgatattt
    11881 ccggcaacga cgatcacttc ttcctccggt tccatagtag atacttccag atccatcaaa
    11941 agctgtagtt gttccagatc cgtcgctgta aaatcagaga ctcttgctcc taagcagaga
    12001 taaaattctg gtcgggcaat caattcagtt tgataaactg ccatatcttc ccaaagttgt
    12061 tcgacagctg tcatataatt ggctgcagtt gttttcatac atgaagcatg tccacgttgg
    12121 accttgataa atcctttttg atcgatgatc cgaagacttc cagcattttt tgaacgattc
    12181 tgttcgccgc atacataaaa gaaaggtgta ttttccaata agcaaacttc atttttattt
    12241 aatcgcatat cctctgcagc aagctctagt gcttcttctg ttgtgatctc cttggttaga
    12301 tcggttgctt tcacttccgt agaaccagta gcgatagccg ttacttttga agtctgtgga
    12361 tcgatttcta cgtggacttc gatcgattct ggtgtagcgc ctgattcaat cgctttattc
    12421 atcgcttcat ttttcaacga tcgtatatct tctttacttg gcgaagggat gatacgttct
    12481 acaacatctc ggaccattgc aagtgctaca ccaatagaag aaatcacttc ggcgttttca
    12541 ggaatcgaat atttcacacc cattttatta ctaaagtaag taatcaatga ggcagcgccg
    12601 ccccctacac ctacaagaga aatctgatct ttctccaagt gatacttgtc tgccaattct
    12661 aaaatcaccg gttcaatctt agcataggat ttttccatga tctgttctgc gatgtcttca
    12721 accgtcgtat gacaataatc agctaatgct tggatggctt ttcgtgcaga tgggacattt
    12781 ccataagaaa aatgttcttc ttgcactaat ccaaggacat tggctgcaca tgtatttgta
    12841 atagtcactt cttctccgtc ttccatcact accttgacgt aatcagcagg gtctccttct
    12901 ttaggagaga aaaattcaac cttaggacct ttgatttttt ctgtctccgt aaatactgca
    12961 taatctagac cggcaatatg tgcagaacgt ggaccgacat cgatgattcc tgactgatta
    13021 gcccgtacca tcgaaccgcc tgccacacct agaacccgaa catctaatga agagatatat
    13081 gttgggtggc cacctacaat tgaataatcg attgctggac gtccattttt aatgaccccg
    13141 atattggttg ttgttccgcc tacttcaaaa taaacaccat tagacgctct caagtacatc
    13201 aaagagccca tgactgatgc tgctggaccg gaaagcatgg ttaaaacagg tcgttttttc
    13261 atttcattga tttccattac tccgccgtct ccgcgcataa tcatcaaaga aacattgacc
    13321 cctgcttctc gtacggaatc ttctgtagag gttgccgtat ctagcatttt tggtaaaata
    13381 gatgcattga ttgctgctgt ccgagttctt cttgtcaatc catataattt tgtgatgtca
    13441 gaagccatcg ttgtagggat cgattttacc gatgctgctt cataaacaac tcgttctggt
    13501 tccccattat ctactccaaa tgccatagaa gaaaccaaga cctccgctct ttctctttct
    13561 aaagaagaaa tcgtttctga aacacggtct acatttaagt gtttgacagg taagaatgca
    13621 ttgacaatct ctattttttt cttattccct aaatcgatat cgttcaatct agtttgtcgt
    13681 tttgctaaaa aaccttctaa tccacctttt gccatcccga ttacgccgac ttttgctacg
    13741 tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat gtgcaacaaa cacaacatct
    13801 tctggactga tattattttc cagcaaacaa ttttgaaaag actgaacgac acctgcggct
    13861 actccacgga catcatcgtg ggttgttttc actgaagatt ttccgatgat ttcgtgcgtc
    13921 gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa catcaattcc cattcgcaca
    13981 aaacgtttca ttttcttttc ctccattcat ctaatcgttt ttatcgacgt tttgtttact
    14041 aggcgttccc gtacaataag aacgtgatca ggcataagat cgctccgatg atccaaccag
    14101 ttgggatgga tttcttcata tattctcgag aagttacttt tgtataacct aatccccaag
    14161 caacccaaga ttgtgtaata tctaaatgct gaggagcaat cgttgtgatt gcaaataacg
    14221 ggaacaaaaa ggctggcgac atcgttggat taacagctaa tacgacagct aaaatagctg
    14281 agcctgaacc gactagattc attggaccgc ggaaaaatcc tagtggtgtc aatatagcaa
    14341 atacgattgc caagactaat ggtgattgcg gtatccaatc tccaaaaata gctgaaaaat
    14401 aaggagaagc atacgcagct gaattattga acatcgctaa ggtcatcaag aaaccaacca
    14461 ttggcgcaac atctactgcg ccatctgtaa ataatttcgc aaactgacga cagatttctt
    14521 tataacctcc gcgcaatttt ccagtaaaca gcaatgccca gatacctgct agaataaaac
    14581 ctaagatgat tggaagatct aaaagaacga cccctaaaac tggtaataat acggctaacc
    14641 atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt tgctgccatt gcatagcgtt
    14701 tctttttgtt cattgatata ttagcgaccg ttagtaccac aactaagctt acaatcatcc
    14761 caatcatacc aatttggaaa tagtcattat aagtatagct ttcagccgct ggattgaaac
    14821 cagcataaat cgtttgatat tgtttgaaat tgacgatatt agcaaagatt ccagccataa
    14881 tcgagcccat aaatgagaac agtgcaaccg gtactgaaat acctaatgac atcaagattg
    14941 gcaacacaat aactgcaata gaaatcactg gtccgattcc tgtcatcgac atgaatataa
    15001 cagcggttac aatacatagt aagctcatcg tgattcttgg cttgtcccct ccaagttcaa
    15061 ctacctttcg aatcagggta gctgcgattc cgctatctac tagaacacgc ccgaaaaagg
    15121 caccaaagaa gacatttacc aagatcgatt tggcgtactc agctggcccc gtcgtataga
    15181 tatgcgtcaa cacatcaatt actccttgcc cttccatcgc cgggtttggt gaaaacgtgt
    15241 ttccgactag cgctaatccc atccatacag tagccatgat ggcaaagccg accatcaagt
    15301 tgtagccttt tacacagtac cagaccatga aaaagaatga gataactaac aaaataccaa
    15361 taataatatc actcatgtta ttcctccgtc cattttgttt ttctgataag caaactaata
    15421 tatcagtcaa actcattcta acttatgtag ttagcgcttt cattaaaggg tgaccataaa
    15481 tgttcagttt tcaattaaaa aaccttaatt atgctatttt agacttatac gttattgtcc
    15541 tgtttcattt gatggaggat tgtaaataaa aaaagaggaa agaacataac ttttcgttat
    15601 cttctttcct ctttataatt gtttagctta tttagccaaa gcttcttttg cttggtcagc
    15661 caatgcagta aatgctgctg catcgttaac agccaagtca gctaacattt tacggttgat
    15721 gtcgatttca gcaactttca aaccgtgcat taattttgag tagctcaagc cattcatacg
    15781 agctgctgcg ttgatacgag caatccacaa tttacggaag tcgcgtttct tttgacgacg
    15841 atcgcgatat gcgtagttgt atgaattcat tacttgttct tttgctgttt taaataaagt
    15901 gtgttttgaa ccgtagtaac cttttgctaa ttttagcact cttttacgac gtttacgagt
    15961 tactgttcca cctttaacac gtgccatgta taattcctcc taataaaaat aaatcttcag
    16021 attctgttat ttgtacttct tatttcattt ttgctagttg ttggcgaata cgtttgaagt
    16081 cgccactaga aaccatgctt gctttacgca attgacggcg ttgttttttt gttttgccgt
    16141 ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc gcctttacca gtacgtttga
    16201 aacgttttgc tgatccgcgg tgtgtttttt gttttggcat gactatattt cctcctcaaa
    16261 atctttcgtt atccgactat cggctgtcca acagccagtt agtcattcaa attacttact
    16321 gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg tccattttcg ctttttgttc
    16381 cactgttgca atatccgcag tttcttcagc taagcgattc aagacttttt gaccaatttc
    16441 tttatgggta atggcacggc ctttgaaacg gatagaagct ttcactttat cgcccttttc
    16501 caagaactta cgtgcattac gaagttttgt attgaagtca ttcaagtcga tcgtaggact
    16561 taagcgaact tcttttacat tgatcacttt ttgtttttta cgcgcttcgc ggtctttctt
    16621 ttgttgttcg aaacggtatt taccgtagtc catgattcgc gcaactggtg gtttcgcacc
    16681 aggggcaact aacacaagat ccaagtttgc ttgttctgca atattcaatg cttctgcttt
    16741 tgtcttaact cctaattgct caccatctga accgatcaat cgtaactctc gtgcacgaat
    16801 gccgtcgtta accatcatat cctttgctat ggtcattcac ctccaaatat tatgagagaa
    16861 agaaaagctt accgaataaa cggcaataaa aaaacgagtt ctcttacaga acccgcgcgt
    16921 acttcaccta atagaaccag tgaaaactat ctagcccagt gacattatca actaaggcga
    16981 gaagcgggtg cttctgcttt tatttctcaa ccatcatagt atagcaaaaa ctaatctttt
    17041 tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa atgattagat tagcagactt
    17101 tctagcagtg attcccgctt ctttgctata ctaaagaaaa acgaggtgaa cccacatgaa
    17161 aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt tttatttaga
    17221 agatgaggag atcttctctg gtcttctgat caacgggggt gaccaaagct atctcgatgc
    17281 caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa aaacagtttt
    17341 acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca atctggagtg
    17401 gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag gtacgaattt
    17461 tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga tggcttcctt
    17521 tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag acagcgaatt
    17581 ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttgaccg gttcgaactg
    17641 gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacag caatcgcttt
    17701 atctcaagat tctttggcgg gactaatcgt caaccaagag caagcacttg tgatcgccca
    17761 agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag tgcaataagc
    17821 atccaaatag ataagaccaa actgatgatc aaggcgattc cccatccgaa aacggaatgg
    17881 gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg aacggtcaag
    17941 atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt cagcaaatta
    18001 ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat ttcaactgct
    18061 tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt ccttttctga caacttgcga
    18121 taaatagcca atgccttgat ttgttctaaa agtactacat tttgttttgt tcctgtaact
    18181 aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt cgttgttttt
    18241 tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat taaaggaaaa
    18301 aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact catgtctggc
    18361 attcgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg cgtggtaaat
    18421 gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata atgattatcg
    18481 actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa agaatcgtac
    18541 tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc actaatgcca
    18601 tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt gatccaaaaa
    18661 ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga catttgtttc
    18721 cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc aactgtaaat
    18781 caaatggcta ctttggtttg gcgaatctct gctaattttt cttcgatcat tttcaagact
    18841 tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat ttggattttt
    18901 ggactttctt tgtattcttc ataccattga tcgtaacgtc tgttcaattc tttgtaataa
    18961 tcgtatagac ttggatcaaa ggacaattgt tcatacgaac gaccacgttt ttcgatacgc
    19021 tccaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg ttttttatgg
    19081 gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac acgaacttct
    19141 gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc ttcataaatc
    19201 gaacggtcca atacattatt gtcatctttc attgcttgtt ttatgcttgc aaaacgttta
    19261 ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata aaataagggc
    19321 aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg ttcagccatc
    19381 atttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat cacgctcatt
    19441 tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt taaatatacc
    19501 cccaatatct agtagtgtca attgtatttt tttctagtca aagaagaaaa aaagtcaaaa
    19561 aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt acttctgaga
    19621 tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca aaaaaattcg
    19681 tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac ttttacgtga
    19741 gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag atcatattga
    19801 tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc tatgcaacaa
    19861 aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt tcttcctact
    19921 attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa ttattcattc
    19981 acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa agcacgattc
    20041 ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact cgtgcttttt
    20101 atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc cagctttcgg
    20161 aaaaaatcgc ccacggtaat cctagtggaa tcgatgcagc cgctactagc agcttgcagc
    20221 cgatttattt tacaaaaggg catcctttcg actacttttc tttgaacatc gatgcttttt
    20281 tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa gatgtcgctc
    20341 acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta ggatacttga
    20401 cgaagcaagc aaaacaagca atcattgaaa attcaccaga aacgttggca cagacaatgg
    20461 atgaatcaca atcacttctg gaaaagctga caatcagcaa tgattttctt aatctattga
    20521 tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt ggacgcggtg
    20581 gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt caagcacttg
    20641 aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc tatgtttaaa
    20701 ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa gaaaaatgaa
    20761 gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt ttatacagag
    20821 acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga tggcaccttg
    20881 caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg taaagaagcc
    20941 ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc agctggatta
    21001 gcctcctctg ccagcgggct agctgcttta gcaggcgctt gcaatactgc tcttaagctt
    21061 ggattagatg atctctctct ttcaagattt gctcgacgcg ggtctggttc agcttgccga
    21121 agtattttcg gtggtttcgt cgaatgggaa aaaggccatg acgacttaag ttcttacgct
    21181 aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt tttgatcaac
    21241 gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga aacatccagt
    21301 ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa acaagcaatc
    21361 aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact aaaaatgcat
    21421 ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag cttaaaagcg
    21481 atggatgcgg tgcgacaatt gagaaagcaa ggcattccat gctactttac gatggatgct
    21541 ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca agaaacattt
    21601 accaaattat ttagcaaaga acaagtaatt actgcacatg ctggtccagg aattgcgatt
    21661 attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact ctatattgcc
    21721 ggagaatatg cagttgttga aacaggccat ccagcagtta tcgctgcagt cgatcaattc
    21781 gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc acaatatagt
    21841 gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat tcgggaaaat
    21901 ccttttcatt atatccttgc tgctattcgc ttgactgaaa agtatgcaca agaaaaaaac
    21961 attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc aaatggccgg
    22021 aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc cttgaatgtt
    22081 ttttacgcgt tgaatttatc tcagttggag attttcaaga ttgcagcact agccaattta
    22141 gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg tggctggatc
    22201 gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca ttctatcagt
    22261 gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc tcctgaagat
    22321 ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt ggtcgatcaa
    22381 gttcaccgtt cgagagaaga taaaatggtg gcttatcagc ttttcttaaa aaatagtaca
    22441 gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt gatccaacag
    22501 atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg ggtcgtcatc
    22561 gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg agccgcaaaa
    22621 tcttctggtg caggtggggg cgattgcgga atcgtaattg ttgaccagaa atctggcatt
    22681 cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt acatgtctat
    22741 agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca ctagctaaag
    22801 cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat aaccctttgc
    22861 cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt acactaagca
    22921 gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa atcaaccaag
    22981 atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca gtcagcgctg
    23041 ccttgaaagt cccttcttta gccgatactt atacgattat gcgacaagaa tatcctcacg
    23101 ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagccaaa gaagctatcc
    23161 gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag ttggttatgc
    23221 ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagaaact caaacagcca
    23281 ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa acgctcaatg
    23341 atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga acaagcttca
    23401 cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct gactgggggc
    23461 aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg gaaatcctag
    23521 cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc ggcgcaaatg
    23581 ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta gaagaaacta
    23641 ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg gtcggcgcaa
    23701 cgaataccgc ggccctacac caacaatctc tgatcttttc cggaccagtg aaggattggt
    23761 gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag agcgtgggac
    23821 aaaagtaaag aacacttttg tctcacgctc taaacacgta caaacggcgg aagtaaaagc
    23881 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaattcc
    23941 ttcttatttc ttggagttaa atgcttctgt cccagcctct tttttcgaat aaatgatgtt
    24001 taatcagctg tccttcggta ttcgttcaat ccgctgattt cacaacctct ccactaaaca
    24061 gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct ccgtccacat
    24121 acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca gatggtccag
    24181 ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata aattgatcga
    24241 atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc ggtgtagcaa
    24301 tataacctgg tccaattcca ttacattgga tattgtattg accgtattca gaagcgatgt
    24361 ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc gtttcgcgtc
    24421 ctaattcact catcatggag cagatattga tgattttacc gccgcctttt tcgatcatat
    24481 ccggaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg attacttggc
    24541 ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct gcattgttga
    24601 ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct tgaaccgctt
    24661 cttcatcggt cacatcgcag acataacctt ttgcatcgat gcctgcttct ttataatgcg
    24721 ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt gctcctgctg
    24781 tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg actaatgcta
    24841 cttttccatc taaacgaaac atatccatac taaattccat cattttctct cccttatgct
    24901 tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt ttctccgcac
    24961 attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga ccagcttggc
    25021 gaaataatgg cttgttcatt gtctacaact aaatgttttg tttcatctgg tttgcccatc
    25081 atgtggaaca cacgtgtatc ttcattggca taatcaaagt agacataagc ttccatacga
    25141 cgttcatgtg tatgacaagg catcgtattc caagcgcttc ctggttctaa aatcgtataa
    25201 cccatttgta attgacagct ttcgcataca tttggatgga tgtattggta gattttacgt
    25261 tcattcagtg tcaggccttc accagtctcc attggtttta cttggtcgat actgattttt
    25321 acatttggat atttatgatg agctggaaca gaactaatat agaatttagc tgggttttca
    25381 gcatcttgag aagaaaatac aacatgtcgt gtttcttttc cgatatagta tccgtcttgt
    25441 tttttcattt cttctttttg cccatcgatc tcgatgaatc ctggtccacc gatattgatc
    25501 acacctagtt cacgacgctc taagaagtaa tcaacaccta gttctttgtc taatgtgatt
    25561 tctaattctt tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt gtgcgtataa
    25621 gtcaaactga ttttccctgg ttcaaatacc ttttctacta aaaattcttt tctcaattgt
    25681 tccgttgaat aatgacggat atcttctgga ctgtgtgtat aacgtgtttc catttcttgc
    25741 attttcttct actccttcta tcgaatgatt tttccagagc cgcatgctaa aaattgtgcg
    25801 acttcttctt gactaaattg gttgcagtcc ccatggatcg tatgcttcaa ggcagaagct
    25861 gccgtagcaa aatcaatgat ttcttgcgga gacatattgg ataaaatccc atggagcacg
    25921 ccacctgcaa aagcatctcc gccacctact cgatccacga ttggggtgat tctatggact
    25981 tgggattcca catattcatg gttcatccac aatgtcccct tcaattcatt ttcacttgct
    26041 gaatgaacgg tacgcttggt cgaataaaac acctgaatat tcgggaactt ttcctgcatt
    26101 ttttgataat aataaattag aggttgttct tctgcttctg gcgctttctc gatacctaac
    26161 aaataaagag catccatttt tccagctgag cagatatcta ctaaaggtaa aagctgatgg
    26221 attacttcgc tggcttcttg ctgtgtccat aattttccac gatagttgat atcgaagctg
    26281 atcaggcagc cagctttttt tgcagcttct atcacctgtt tcgtcatttt ttgccatttt
    26341 ttagacaaag ctggggtgat tcctgaaaca tgaaatatat ctattccttg gaataactcc
    26401 tctagcgacc attccaaatg atccatttca gcaaaacttg atccggcacg atcatagatc
    26461 actgaggcgg cgcgttcgcc actaccagtt tccatataat aagtacctag tcttgatcca
    26521 ccagataata cctgtttcgt atctactcca taacattgca agtgcttttt gactgctttt
    26581 cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt catgtccgta attagctaaa
    26641 gaaattgcta cgtttgcttc ccctccgcca taatgggcag cgaatcgttc acttgaatcg
    26701 atacgggttc cctgctctgt ggacaatcgc aacatgattt ctccgagtgt cactactttt
    26761 cccataagca gctacctctt gatttctttc atttttgctg catactgttg cgcgacttcc
    26821 gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc tactccgact
    26881 gttacgacac cagcatcgaa ccactctttc atattgtcca gactcacacc gccagttggc
    26941 atgatattga cttgaggcat cggcgccttg aaggcagaaa tgatacttgg tccataagcg
    27001 cttccaggaa atagtttgac gatatctacc ccgcttttca gcgctgtttt gatctcggta
    27061 atagtcatac atcctggtaa gtaaggaatt tgatacagat tgcacatctc tgccgtttct
    27121 tgatcaaagg aagggctaac aatatactct gcgccagcta agatagccaa gcgagctgtg
    27181 acagcgtcta atactgtgcc tgcgccaatc actatttctg gttggtcttt gtagaatgac
    27241 aaaagttctt tgattacttg atctgcttga gggactgtaa aagtcaactc aataccagtt
    27301 agaccacctt tgatgattgc gtggcaagca ttcaaagctt cttctttgct ttttccgcgt
    27361 accaccgcaa tcactccggc gtttttcaat cgagataaaa tatcaatttt tttcatcttc
    27421 tccacctatt tcctatttag aaattttatt ttataacttg gaaatctata cccatattat
    27481 acggcagaaa agaaaaattg caaacgcttt tttcattatt tttgaattct ttgctcgatt
    27541 tcgctttttg tttgcttgag ggcactgatt atttgttctt ttgtctcatc tgttaaacga
    27601 tatttcggca tacttacact aaatgcacca atgatttgtt cgtttttcac gattgaggct
    27661 cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg ggtttctttt
    27721 accgttttga tttccttttt caaccgtaat gcattggtga tcgtatattc tgtgtaaggg
    27781 atcaacggat gcttttcaag ataatcagtg tactgctctt cactgaattc tgctaatacg
    27841 gctttgccca tcgcagaatt gtataatggt cgggtgattc cgatttttga agacatgcgg
    27901 atcgtttgat gttttggatc tagtttgttc acgtacaaga tctcattgtt gtccaatacc
    27961 cctaagtgga tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg taaagtcaac
    28021 tctaccaaat cgatttgttc aatgttttta ttcgcgtagc gaataagctt tgcacctaaa
    28081 cgatagtttt tctctgaatt tttatttacg tagccgatca tcaacaaagt atctagtatt
    28141 ttcaagactg tagaagaggt cattcccaca ccctttgcga tcgtctgcaa agagacatct
    28201 tggttttccg ctaaatagtc tagtatttta gaggctttga tcaatacagt tccataaggc
    28261 tttttatttt ccatataata ttctcctatt tctttataag aaaaattata gcggaaatac
    28321 catcaattta aaagagatat ccagatataa actaccattt ctctttacag gcaatttaat
    28381 gttgaagatc cgcttcgttt ttacatcaaa ctttgatata aagcaatgat ttcttctact
    28441 gtcgtttctc ttggatttcc tggtgtgcat acatctcgca aagcatcttc tgcaattgct
    28501 ggaatatctg cttcttttac acctaattca gaaatggttt ctgggattcc aaccgctttg
    28561 ctcaaccgat cgatctcacc acaagccgct tctcttacat cttctaaaga catttcagct
    28621 gctcctttaa tccctagaac aagagcgatc tcacggtact tttctccagt atattctttg
    28681 ttatatttca tgacagttgg caaaagtgct gcacacgcta cgccatgagg aatgttgtac
    28741 catgcactta acgggtgagc catcccgtgt accagtccta aaccgacatt tgagaagccc
    28801 attcccgcaa tatactgtcc taatgccatt ttttctcgcc cattttgatc gccatctaca
    28861 gaagcttcca aagaatggcc gatgatttcg attgctttta aatgcagcat atcagtcatt
    28921 tcccaagcgc cttttgtgat aaacccttca atagcatgcg tcatagcatc catacctgtt
    28981 gatgcagcca attttttcgg cattcccatc atcatatcgc tgtcgatgaa cgcgacgatt
    29041 gggatatcat gaggatcaac acaaacaaat tttcggtgat ttacttcatc tgtgatgacg
    29101 tagttgatcg ttacttctgc tgctgttcca gaagtcgttg gtatagctaa tataggtaaa
    29161 catggatttt tcgtatcagc aacaccttct aaactaatga cgtctgaaaa ctctggattc
    29221 gtcaaaataa tcccaatggc ttttgccgta tcgattggcg aacctccacc gattgcgatc
    29281 aaacagtctg cctctgcctc tttaacaaaa gccactccat cttttacatt ttggatcgtt
    29341 ggattaggta cgatcccgtc gtataaagca taagcgattg ctgctttatc tagaagttct
    29401 gttacttttg ttgctatacc gtgctcgatc aatcctttgt cagtaatgac agctgctttg
    29461 ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa tcgctccttt tccaaaatac
    29521 gatgtttcat ttaaaatcat tcgattactc attttctatt cctcctaatt tgtattattt
    29581 ttcacaataa aaacgaaaga accccaagca accggttgcc cagttgcttg ttgctacagt
    29641 tcaaagactg gcatcaattc ccaactgact ggagaggcat ctggatttgt ctccatgata
    29701 tcttccatgt acttccacca tttttgattg atagatgtat ttgccatttt ttcccataac
    29761 ttttcatctt cgatcactaa gtaaccaaat aacaaacttg tactcggatc aagatagatc
    29821 gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca tttctggcca aagttcatca
    29881 tgtctttttt tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa agcttttttc
    29941 accatgtcag acacctgctt tcgtatgaac ttcctccaaa taacctgctt ttggagtgac
    30001 accaaacgca tctgctaaat gccacaggtt ttcgtccgtg attgtttgca agattggtcc
    30061 ttgcgctttg acgtacgtat agacttctgc tgccttttct gctgtctcga tcaaaccaaa
    30121 gacttcatcc atgtcttttc ctgacccgta gattccatgt tgtggccata aaaccaatcg
    30181 agtttctttc attttttcag ctgttgcttc cccaatctca tttgttcccg gaaccatcca
    30241 cggaatgatg gaaattcctt ctggaaaaac aactaagcat tccgtacaca tttgccatag
    30301 cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta atagatgact
    30361 agcatggcaa tgcataacga tccgattatc tgggtctact tctaaacggg cgatatgact
    30421 catgaaatga cttggcaact cgcttgtagg aaggcattcg ttttccagcc cccacaaaag
    30481 ttccagtgtt ttgccatctt cagcaactcg aacgattcca agattctcgg caggttcgct
    30541 cgctacattt ttaaaatatc ttcctgaacc agttacaata aaatagcatc ctgctaaacg
    30601 gctggcatca aaaatcatcg agatgcggcg gatcactttt tcaggatcca agaacggtgt
    30661 gatctcttct tcttttagta aataagaaat attcccgcca tttctttcat cccatcccaa
    30721 gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt gtaaaatatc
    30781 tatttttttc aatatagcca cactcctatt ttcttaattc caacacgtct ttttgatatt
    30841 cctggacttc ttccagccaa tccaaaccaa ctggaacttg atttttgtaa cagaaataat
    30901 tccatacatc ggcatacggg aaatctttca attcttccgt ataagccatt cttgaagtaa
    30961 agtcaaaagc cagctccatt tcttccaatc gctcaactgg ttccagcata gctttcagca
    31021 gtgctttttg tgtatttctc gtaccgatga cccaagcagc gatacggttg atcgttgcat
    31081 caaagaaatc cagaccgata tttgtttttt ccaacaattc gttacggact aattctttag
    31141 cgatttcctg caattcatca tccatcaaaa ccacatggtc tgaatcccaa cgcactggac
    31201 gggaaacatg caacatgatt cctttgctga ataaagctaa tgaagataat ttattggaaa
    31261 tcacttctgt tggatgaaaa tgtccagcat ctagacaaac aagtttgtca cgagtcaacc
    31321 cgtaacccat ataaaattca tgggaaccga ctgtataagc ttcagcgcct aaaccaaata
    31381 gtttgctttc tactgcatct tgtacatacc gttcatctaa ctcttctgta aaaatttcat
    31441 cgagtgattc cattaaacgt tcccgtgggg taaaacgatc aatcggatta tctttcatcc
    31501 catcaggtac ccaaaagtta ttgatacttg tttgcccagt ttctttccca aagtactccg
    31561 cgattttgcg gcttcttttc ccgtgttcga tccagtattc acgaacttct ttatctggat
    31621 gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga ttgaagtcaa
    31681 gaccgatttc ttcttttttt gcccactcta cccatttttc aaaatgtttc ggctcaattt
    31741 catttagatc gacagattct tctgtatcca aatagatagc atgaaggttc aatttgtgtt
    31801 tgcccggtat caacgaataa gccttctcca gatcttgtct aagctcttca ggtgttcgtg
    31861 cggctcctgg aaaattccct gtcgccatga ttccaccggt caattcgcca tccggtgtca
    31921 agaaaccttt gacatcatct ccttgccagc aatgcataga aattttgatg tcctgtatct
    31981 tttctagtac agcatctgta tctacaccga tagttgcata tttttctttt gcttgttcgt
    32041 aatttctttc aatcgtgttc atacgattac tcctttgatt ttattcacgt accattccat
    32101 cgtgttttgc ttattttcat ctgggtaaaa agtttcacat ggaaacgact gtttgattac
    32161 atttcttgct tgttctatat tcgaaaactg acctgtactg agcatttgca tggcaacatt
    32221 cccgattgct gtggcttcac taggtcctgc ttctacaatg attccggcat aatctgctgt
    32281 caattgattc agtagacgat tattcgatcc gccccctacg atgtgcagct tttccaattt
    32341 cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg taacaaagag caagattatc
    32401 atacacacaa cgagcgatag ctcccgatga gacagggact ggctgctctg tttcaaaaca
    32461 gtattgctga atctcttcta tcatatttgg gggattcaaa aatcgagggt cattgatttc
    32521 catcacttgc tgcagcggtt tttcttttgc agcaagctct gccaattccg cataactgta
    32581 ctcgtactct tgcattcggg caacttcctg gatcaaccac atccccatga tgtttttcaa
    32641 aaaacgcacc gtgttatggg caccccattc attcgtataa ttttcagaaa aagcattgcg
    32701 agaaacattg gcgatctttg tttcaatccc taaaagcgac catgtcccac tgctgatata
    32761 accccaacgt tctccttgac ctggcgttcc taatacagct gacgccgtat catgacttgc
    32821 tactgtgata aaggtcacat ctggcaaatc atattttgaa aattgttctt tttgcagatc
    32881 tcctagtatc gttcccggct ctgtcaatgg tggaaataat tccttcgaga caccgatttt
    32941 ttcaagaagt tctatttccc attcttttgt ccctgcattc agcagctgca tagtagatgc
    33001 attagttttc tccgtcacca tgttaccagt aaagacatac cctaaataat caggtatcag
    33061 taacaattta tgtgcttttg ccaaattttc tttttcttca gcaaacaatt ggaacaaggt
    33121 attgaaaggc tggatctgaa tccctgtctg ctcatacaac ttatctagag aatagatttc
    33181 agaaaactgt tctactgctc cattggtacg cttgtcccga taagcaatcg gatcatttat
    33241 ccgatcccct tgttcatcaa tcagacagta atcgactgcc catgtatcga tccccgcaaa
    33301 aaggcgatca atccctgtct gctttaattt ttcaaatcca atcaaaatct ctttgataag
    33361 ataatcgatc ttccagcgat cgtgaccgtc tttgttccga aagccgtttt tgaaccggtg
    33421 tacttcttcc aagatcaaag aaccttttgc atccctcgta cttttcatta gtcgtccgct
    33481 tgatgcaccg atatcgatgg caacaaatgt tggattcatt tctccacctc ttttacttta
    33541 ttgttgatta gcactttatt aataaagcgt tatcatttat tgacaatgct atcataatac
    33601 agaatcttat attttgcttc acactatttt ggtatataat tgcattatct tgacaagttg
    33661 ggagtgttta catggattac tgggaaaaag ccaaccactc atggtcggaa gactcaaaaa
    33721 gagtgatctt gacgcctaca caaagaagca ggaatttatt tttttatatc caagaaatcg
    33781 gttacttcaa agcaccaatc ccttactata ctgaacgagc acatttacct tcttacttga
    33841 taaagtttac tttgtctggc aaaggttcgc ttacatataa tgataatcag caacagctaa
    33901 aagctggaga cttatttttt attgactgcc aagaatatca gcgttatcaa acgatcagcg
    33961 aagaaccatg ggaaatggat tggatccatt tgaatggtca gaacgtccaa tcattttatc
    34021 aggagttcac aaaagacggc cataacacct tccattcaac aggagatcca tcagaaaaca
    34081 agatccacca tttgatcaca gaattgatcc acatgcaaca aggcacgaat gcccgtaccg
    34141 atttccagtc ctctgtagct atccacgagt tgctgaatga actgattttg caaaagtatc
    34201 agctagattt cgaagaagag gatattcccg cctacgttta caaactgaag caatacttag
    34261 acgaacattt caaagaaacg attacacttg atcaattaga acaacaattc ttattgaata
    34321 aatatcagct gagcaaagat ttttccaaat ttatcggttc tccaccaatc gattatttga
    34381 tcagcaaaaa actttcttac gctaaagacc ttcttcggta tacagaattt acggttcgaa
    34441 tgatttccga agaaataggg attgagaatt ttgcttactt tagccgcctg ttcaaagcaa
    34501 aaacaggact atcgccgaga gactttcgta aattcagctg attcatataa tatgtacgtc
    34561 attttttcaa aatattttga aatatataga taaaaataaa ataaccgcag tttctgcttc
    34621 tatgtaaaca acagcagcta ctgcggttat tttttagtga tcattctcat tgttcatctt
    34681 caaaatgttc ataatgaacc aactgtttct gtgtatcacg tttcaaaaat tctttaggcg
    34741 ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg ataagaagaa aaaccacttt
    34801 tactatatac agcactagag ctgcggtatt ctcgcaacaa gtgcttggca tagatcagct
    34861 ttttcttcat tacatactga tgaaacgtaa atccagtgta ctttttaaat tctcgtgtga
    34921 tatggtattt gctgacaaaa aactgctttt ccatttcctc taaagaaagc ggatgagata
    34981 aattttttga gatatattgt aacatttgtt ccacacgctc attaggaact acagcattct
    35041 gttcttgcgc actctcttct ttttgtacaa cttgattgag aaaaatcaag tattctacca
    35101 gagcttgttc atattttatg tcttcaccgt aaattttttt atcaggagga tggtcgataa
    35161 atgataattt ttgcttcaat acttcctgtt ctatttgcaa tacacgactc ctcttctctc
    35221 ccattggttg aaaacaagcc gatagattag accatggcgt ggaacgactt tctagaaaag
    35281 aaggggtgac aaagatatag acacgctcga atacatctga agattggcgg acgatacgat
    35341 gcaaatcatt ggaatggatg agcagcacac ttcctgcttt gatcgtaaat tgtcgtccgt
    35401 tcaaataaaa agtcgcttct ccctttaatg tagcatggat ctcatagaaa tcatgatgat
    35461 gatagatcgt tttattttgg ctgacgacat ctttgacatg atagatttca tactgatcag
    35521 aaaccatcgt attgacttgt tcaccgctga ttttcttttg gagcatgtgc cgcacctcct
    35581 tttgttcact tatgcacaat tttattatac cacgtttttt gtaatcaaaa agagctgctt
    35641 ttatatactc ttataaaaaa gaagagacgt ggcatcttct agccccgccc cctccattct
    35701 aaatattagt taaccaacgt cttctttaca ttattcaaga agacttcttc cataacgaat
    35761 cccatcatct tttgtttctt caagaaccac atacaggtac ggacgattct tcatgatgat
    35821 ttctttgatt tttgtgtact cgatcacacc gtctcccaaa tttaccggca cgattttttc
    35881 ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa aacgttcaaa
    35941 agcttcttct accaattgga cttgttcttg ataatcacta cttgtgatca aattggtcgg
    36001 atctaaaatg attcctagat agtccgaatc tactgcttgg atcagctgct ctacccttgc
    36061 taaagaatac accggatgat tcaaacctgg ttctatcccc accatcattt ggtatttttc
    36121 tccagctttt accaagcgac ggataacgga cacagcttca gcaaatgctt catctgtgaa
    36181 attttcttcc gtatattgga tttccggcaa aacacatcca gtttccgaag caaccatcga
    36241 cgcaccaaag gaagaagcat agcgaagata ctgctcgaac tttgtcagca actcttgtcg
    36301 tatcgttaaa tctggatgaa tcatattgat atagcaactt aaaattccta cggtcacttc
    36361 ttgcttttct aaaactcgtt tgacataatt tcccatccca gaattgatct catttgcgcc
    36421 agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa tcccttgttc
    36481 ttttgttttt tctgcaagat cttctatcgt ttggacttcg gaaagatcat gtcctctaat
    36541 tgtaatgttc aacataacta ttctcctaac tcagcatatt gggtagccac atgacgattt
    36601 gcgggaagta agtaatcaaa aacaagacga ctaagataac cccataaaaa ggaagaagcg
    36661 gtttcagcat tgcctctgct tttacgcctc ctacacttgc accgacatat agtcctgtac
    36721 ctactggcgg cgtgatggag cctacacaca gattcatgat tgaaaacaac ccgtaatgta
    36781 caggatcaac ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa atcaagatag
    36841 caggcgccac atccataaac atacccacca gtaataacac aagattgact aataacagaa
    36901 taataaattt attatccgtt acgcccataa tcaacgaact aattgcctga ggaataccgg
    36961 taaacgccat ggcgaaagac atcatggaag aagtagccaa taagaacatg atcgtccctg
    37021 acattgctac tgattgaaac aacatattag gtatatcttt tagttttacc gttttatagt
    37081 aaaacatagc aatcaacagg gagtaaatga cagctatcgc tgaagcttct actgctgtaa
    37141 atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta ggaatcgcct
    37201 cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg tatttattct
    37261 ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagcg cctacgagat
    37321 aacctcccat aaacaaagca gagatcgaag caccaccagc aacaagtgaa tacatgataa
    37381 aagcagtact tggaggaatg accatacctg tcggagctga cgcgatattg accgctgtcg
    37441 caaacttccg gtcatatcct tcttccactt gctgagggat caacactccg ccaatcgcgg
    37501 tagaagcggc aatggccgag ctagagatgg aaccaaataa agcattccct aacacattcg
    37561 tatgagccaa agcccccggc acgcgcccga cgaacaacat cgcaaaatcc actagtttct
    37621 tagctatccc tccattattc atgataatgc ccgagagaac gaaaaacgga atagcgatca
    37681 gcgaaaaact attgatactt cccaccattt tttgagctga actaaaagca gaaacatcaa
    37741 acggtataac taacaataaa gtcatcatcg atgagaatgc aatacttata gcaatcggca
    37801 tccctaatac aagcaacaca acaaatacaa aaaataacac taatccggct tctattgcca
    37861 ttttattcct ccatttttat attttttctt gaattgctgg cttgttgctt aatgtatcga
    37921 ttagattgat caggctatag ataatgatga gtacccccga tacaggtact gacagataaa
    37981 catatcccat aggaatactt aaagaaggcg agatctgccc cattgtcaat gtgacagcat
    38041 tccatccgcc atacaccatg atcatcgcag caaataccag aaataaactt tgaacaacaa
    38101 cttctaaaat tttacttttt ttgccagtta atttttcact taaaaccgtt acggctaaat
    38161 gtgctttttg tcccacaaca tacgcggaag acaacatggc aaaccagaca agaccgaatc
    38221 gaacaagctc ttccgtgacc gtatttggat ttcctaaaac cgttctggaa aatacttgcc
    38281 ataaaacaac agcgaccatc ccaacaagca aaacagctcc caggatctct aataccttgt
    38341 ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg atcttttgat aaaggtcttt
    38401 gtaggtagga tcgtttttga atatatcatg aagtggggca actgcctctc gaaacggtgt
    38461 attatctaca tcattgaatt ggactccatg ttcttcttgt gctaaagctt tttcttcttc
    38521 aattgctttt gcaaaagctt caatttcaaa aaccgttgat tctttcgctg cttcttcaat
    38581 ggctttttcc tgctcatcag ttagctgttc tttgatggca tcattcatga tgatgatatc
    38641 tggcacacgt gtatgctcat cataggaata gtatttagcc acaccaccat gtccagctgt
    38701 atacaagaca aattcattgt tttctgatcc gttgatcaaa ttggattgaa gagacgtgta
    38761 cacttcatca cttcccatag gtacgggcga accgcctaaa agctctacca ttttgatagc
    38821 ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa tcttccggcg tatggatcgg
    38881 accgtctacc atataaaagc tgcgctgacc agaatcataa taggtcaatc cggtaaatcc
    38941 taaatttttg gtcgattggt agattggctc cattatttct tcattatcca tcaccttaaa
    39001 gaaatgttct tcatcattga atagataagg aactgaaaaa atcgaataat ctttagagaa
    39061 actttccaac gctgaggcac ttacttttgt gaaatctact gctcctgact gtgtcaattc
    39121 gatcagttca cgttctccac ccagttgtcc atctgggaaa tactggatct gtacggaacc
    39181 atctgttttt tcttttacta aacgcccaaa ttcattcatt gcatcaatta ctggctggct
    39241 gttgcttgca taagcaaagc gcaaggtaac tatctcattt acatctgcat ctgcgacagt
    39301 tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc taagcgaact
    39361 tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa caataagaga
    39421 cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt gctgtgattc
    39481 cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa acgttttgaa
    39541 cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa gcgacttctt
    39601 tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa gcaaatcctg
    39661 cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc cataaaccgc
    39721 tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg atcaataatt
    39781 tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga atagcaatcg
    39841 tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt ccttcaaacg
    39901 tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata tggatcataa
    39961 actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg atttttgcca
    40021 aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt tccggtccat
    40081 atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg acccattctg
    40141 cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac aagtaagcca
    40201 tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct tccatacgat
    40261 cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc tggtaatttt
    40321 tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag cttttgtcat
    40381 ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg atcgttgtca
    40441 ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca aaaatctctt
    40501 ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc cttaatatag
    40561 cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa aattgaccta
    40621 aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga acttggagaa
    40681 acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg ctttcttttg
    40741 cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat ggttctttcg
    40801 gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct acggttgtat
    40861 ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg acagattttc
    40921 ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt tcgcctttca
    40981 gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag tcatatttag
    41041 ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc ggtaaaagaa
    41101 tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt gcgactgagt
    41161 gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa gcgttgtgca
    41221 ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag tcaaagacta
    41281 acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact gaaacatagg
    41341 attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat gctggaactg
    41401 acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa tctgctaacg
    41461 gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact tcagaaccag
    41521 tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg atcttatatg
    41581 tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg tctgggtgtt
    41641 catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca agagcgatta
    41701 ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta tttgtcgatg
    41761 ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt cgtgccaata
    41821 cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa acgatcatga
    41881 cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca aagaagattt
    41941 ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt ttgacattga
    42001 ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa ccgcatccca
    42061 aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct gcagatggtg
    42121 tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc aactcttcgt
    42181 tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata ccttcacaca
    42241 aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga gaaagttttt
    42301 ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc attttcgttc
    42361 cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt ccaacgattg
    42421 atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt tgcaattcat
    42481 ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct gcatagattt
    42541 ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca aatgttttcg
    42601 aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata taagctggta
    42661 cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac ttgaccattc
    42721 cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc atagaagtcg
    42781 cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg gcagcgatag
    42841 cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt ggatggaaag
    42901 caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc gttgtggaag
    42961 tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca atttcaatca
    43021 accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg atattatgcc
    43081 aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca ccgcggcctg
    43141 tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc attgccattt
    43201 catgcacgat atggtctact ttttcttgat caaagctttc catttctttc aatgctgcat
    43261 ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc gttttttctt
    43321 tttctattac tttagccatc attatcctcc taatgggtca tggttatttg ttaaataatt
    43381 cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg atttttttca
    43441 cagttatttt taaatataaa attgctttat ttacaggtgt ttgtatagaa aaatatataa
    43501 agttatttat tattatttat atgacacacc cgattattca atattgagca atatatccca
    43561 tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct
    43621 cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt
    43681 tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta
    43741 gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg
    43801 tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct
    43861 gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct
    43921 aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt
    43981 gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta
    44041 attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt
    44101 aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct
    44161 tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt
    44221 ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt
    44281 tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa
    44341 tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga
    44401 cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca
    44461 aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc
    44521 aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt
    44581 tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg
    44641 actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca
    44701 aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac
    44761 gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca
    44821 aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat
    44881 aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc
    44941 cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc
    45001 cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc
    45061 cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg
    45121 gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt
    45181 ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa
    45241 cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac
    45301 aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc ttccttctac
    45361 tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt
    45421 atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa ggagggaaaa
    45481 atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa aggacgttgg
    45541 ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac tattcttctt
    45601 attatgattc ctattatctt gtttgccacc accatatatg atggatcagc gagcgtctca
    45661 gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat ttccagcatc
    45721 attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt acgtggacaa
    45781 aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt gtttatcggc
    45841 ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg tttgctatta
    45901 gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat ttactacgat
    45961 atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc aagtcgaaaa
    46021 ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat cggctggcat
    46081 atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata catttctgca
    46141 acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt gtgacttgtg
    46201 accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa cattcagccc
    46261 attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta tagttccagt
    46321 aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc cggtaatcga
    46381 tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc taccaagaac
    46441 cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg cagcaaagca
    46501 acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt attggtcgct
    46561 aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag aatcgatgaa
    46621 taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc tacccatggg
    46681 ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat ggtgaagacc
    46741 cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc atttttcgga
    46801 atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc aaatcctgga
    46861 agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg tgctccccat
    46921 cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct tagcaaaaag
    46981 ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc gtctacgatc
    47041 gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac atctgttcct
    47101 gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc attcacacca
    47161 tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat ccggatcttg
    47221 tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc taattcttcg
    47281 tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt caagatacct
    47341 aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat gactgttttg
    47401 atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg tggatcgatc
    47461 attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga aatatcttgc
    47521 ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg tttaccaaag
    47581 cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca
    47641 tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac tgttgtcatc
    47701 atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc ttctaattct
    47761 gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt tggattacct
    47821 ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag tgcagccatt
    47881 ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac ttcatcttct
    47941 ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag
    48001 cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat gatcgcatgt
    48061 ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac agtcaaggtt
    48121 tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag gagtggttct
    48181 ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat catactgatt
    48241 cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt attttcgttc
    48301 aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac gatcgctttc
    48361 ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc acctaatgac
    48421 tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc tccagtcaac
    48481 gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc agaaatagca
    48541 tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc aacagtggtc
    48601 actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa ggattccagt
    48661 gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac aacgatagcg
    48721 atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt ataaaaggaa
    48781 atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt
    48841 ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt ttttcgccgt
    48901 tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac cttctttact
    48961 tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa
    49021 aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa gggcttatcg
    49081 attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga
    49141 tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga atttctcgat
    49201 agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac atgcgtgtag
    49261 agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca
    49321 agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg aataggaaca
    49381 agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa
    49441 ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt tatttgaatc
    49501 cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt gtcataaaat
    49561 ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta tttcacgaac
    49621 atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat
    49681 aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga ttttggcaaa
    49741 tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt aatgaaaata
    49801 tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat cgtttctaat
    49861 gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg tacttggttc
    49921 aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg gtgttcttca
    49981 attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt cagatagact
    50041 aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg caatccagct
    50101 gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg atctgggtct
    50161 tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc aagaatctct
    50221 agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc
    50281 actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt ggcacgtttc
    50341 gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag aaggacttct
    50401 aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac tcgtgtatta
    50461 ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa atgtttatct
    50521 tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg gtcttttaat
    50581 ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt tgttaacata
    50641 tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt tctgttggca
    50701 aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact accctatttg
    50761 tattttttca ttgttttagg catactagta atagatagtt cttcattgaa aatagaaaaa
    50821 gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg
    50881 ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt atcggtacga
    50941 gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat ccaacacaag
    51001 ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa ttgcgacgtc
    51061 agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc cgtgaaaata
    51121 tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa acagctgaaa
    51181 aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca catgaattat
    51241 ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat caagatatca
    51301 tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc
    51361 ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc catgacatgg
    51421 atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt gtgatccaat
    51481 ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg
    51541 gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa gttatgctaa
    51601 atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg
    51661 aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa ggatttatcg
    51721 atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac attttgaata
    51781 aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa cgcattttaa
    51841 aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta ggtatcctga
    51901 ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat cccgcaacta
    51961 ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct atgcagcagt
    52021 tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt tatatttcag
    52081 gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta ctgacacgtt
    52141 ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca atcccttctc
    52201 ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct gcgattatcg
    52261 cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg atgaaaaatg
    52321 tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc caatccattt
    52381 tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt ttagctgccg
    52441 tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt ttaggcgatt
    52501 tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt acgatccctg
    52561 taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac cggctgacac
    52621 cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga ccatggttct
    52681 tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca atacagatga
    52741 agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag caagtctagt
    52801 tgccggaatg gttgaacatt atacagataa aaataccgca atcatcaaca atctggctac
    52861 tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag cggctcgcta
    52921 cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc
    52981 gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt tcccgtccta
    53041 tagttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa aataccatct
    53101 atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg tagatacttc
    53161 ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg gcttctcctt
    53221 caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag caggaaaaat
    53281 ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc tagtcatgtt
    53341 ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag acaacaagat
    53401 attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata ccatcagtac
    53461 ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac catcagtagt
    53521 agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca attggataaa
    53581 atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta tatctttgca
    53641 caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc agttgtcggg
    53701 attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt gattcgtttg
    53761 gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat cattggatta
    53821 ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt accgatcatc
    53881 aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt cggcaaaggc
    53941 atggggatga ctgctagaca acgactattc atggtagagt taccactctc tgtttctgtt
    54001 atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc gatcggtgca
    54061 ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc aacagagggc
    54121 acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat cacggactgg
    54181 cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg ctgatgttat
    54241 tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt tagttctgtc
    54301 atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga gttgcttttc
    54361 ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt catatctgca
    54421 tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat tttcccatca
    54481 ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt ctttgaattg
    54541 ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca tcagtcctcg
    54601 attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg gtaacttcga
    54661 tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac atatctagcc
    54721 attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg attcttatgg
    54781 actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa gagcaccccc
    54841 gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa agatatcgat
    54901 ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca cgactttaac
    54961 ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc gctctgctaa
    55021 aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc atgccaacga
    55081 atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt tcaacatact
    55141 tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt ggcgataatc
    55201 gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga ttttccttgt
    55261 cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta aactcaaaca
    55321 atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa taaatccaag
    55381 aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt gttaactatc
    55441 taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata aatttctaca
    55501 actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag cctgcgtgag
    55561 tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat atatggatct
    55621 tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg cttgattttt
    55681 cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat caaagagctt
    55741 cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt
    55801 tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca
    55861 gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt tcagtatgat
    55921 tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta ggattcatcg
    55981 ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac ttgattaagt
    56041 acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt ttttacatag
    56101 agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac tcctttacga
    56161 ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc aaaggtcgaa
    56221 tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg ttgcagtgtg
    56281 acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa cggttattca
    56341 aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc
    56401 tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt gtgaatcacc
    56461 aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag gctgagattc
    56521 ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct gaaaatatag
    56581 gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc aaaatcaaaa
    56641 tgttttacag ctaaatagga agaataataa catttcatac gctgttccca tctttaatgg
    56701 cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc ttcaattaag
    56761 agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg gtagctatag
    56821 gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg
    56881 ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt
    56941 gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat aattggattt
    57001 tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa atgcttttta
    57061 aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac atgcatcgca
    57121 acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca
    57181 ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc atcattttct
    57241 aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa aatgaacata
    57301 acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac aaacaaaatc
    57361 gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt gcttaaccat
    57421 ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc aaatggaatg
    57481 aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa aataaacagc
    57541 aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag tcgatcccca
    57601 cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc aagaactgga
    57661 aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc aaattgaagg
    57721 acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa aacagtgact
    57781 tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac gaagaagaaa
    57841 cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct tggcttctta
    57901 aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga accagaagaa
    57961 aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg aagtgcagta
    58021 gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat cagtaacgag
    58081 atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc cctcagccca
    58141 cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac ttgatgaccg
    58201 tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga tactgctact
    58261 gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt ctttccaaga
    58321 ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat gatttgacga
    58381 tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg tagcgaatac
    58441 cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt accatatgca
    58501 atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt taatttgtgc
    58561 tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa gtaatccaat
    58621 ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat ttatagtaaa
    58681 caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga aaataattgg
    58741 aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa aaatctaaca
    58801 tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg acttagaaaa
    58861 tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg ctgctttcta
    58921 cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga taaacggtgt
    58981 ttaaaagctt gcatctgcgg taataagccc aaccaaatta aaaacataag aagctgtttt
    59041 tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct tggtacacgg
    59101 tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg aggaactatt
    59161 tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct ctttttttaa
    59221 tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc ttcgatgaac
    59281 ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat gatccagatt
    59341 gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa gtttaccgca
    59401 tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac ttggatcgga
    59461 tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat ccctttaccg
    59521 acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa ggcgatcaag
    59581 acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa gaaaatattc
    59641 cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag catacgagca
    59701 acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata gtattctgtc
    59761 gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt ataaaacggg
    59821 gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag ctcaacaggt
    59881 tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat gacttcgcct
    59941 agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat accagctttg
    60001 atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct
    60061 ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag tgctggccaa
    60121 tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg taattcattg
    60181 ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt tccattcagc
    60241 attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg atatttttct
    60301 aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga atcaaagtcg
    60361 atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct
    60421 agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat gatgccattg
    60481 atattattaa caacggcatc aatttgatct agcaattcat caatagcctt cgtgtcttca
    60541 ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag caagggtttt
    60601 aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact tgtttgcaat
    60661 ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat tccgtcaata
    60721 aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt accatcttca
    60781 atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg tccaattgac
    60841 ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag ctgagaggca
    60901 cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt cacatctggc
    60961 aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc gattccttgg
    61021 ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg cttaccggca
    61081 ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg atctttcaac
    61141 tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac tttagggatg
    61201 taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag ttgactttgt
    61261 aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc tgttgataaa
    61321 atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta acctatctca
    61381 ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt gatttgcgat
    61441 tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat tttttcattt
    61501 accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag cagatcttct
    61561 gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac tccgtcttct
    61621 aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg aagcgattcg
    61681 atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt gtctgcactg
    61741 taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc tttgatgtta
    61801 aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg
    61861 tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt gattgactgc
    61921 atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt tcatactttt
    61981 ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa ttgtctcatt
    62041 gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta tcatgaaata
    62101 aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg caaaaaagct
    62161 aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt ttttattcct
    62221 gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt ccttcaacta
    62281 gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa ataaaaacat
    62341 gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca tctagatatt
    62401 cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa tctagatctg
    62461 ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg ccggtttctc
    62521 ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca aattgcagac
    62581 ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt accttttcct
    62641 catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga agctggatca
    62701 gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc ggctggattt
    62761 ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt ccggctgctt
    62821 cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac acgacttctt
    62881 ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga acagttgtga
    62941 catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca taagggaatt
    63001 tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa ggattcacac
    63061 tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga tgagcatcga
    63121 tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt cgcatggtca
    63181 ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa aaaatgtctt
    63241 ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat
    63301 tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt ggaggaattt
    63361 gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg
    63421 ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact
    63481 tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg
    63541 cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag cacataaagg
    63601 tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta atgtaaagga
    63661 ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt cttctagcag
    63721 aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc ttttttatat
    63781 gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag tgcttcgata
    63841 cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc caaatggcag
    63901 accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag gataacggag
    63961 tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag cttaggttta
    64021 gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt acctagatta
    64081 ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac tggagaaaga
    64141 tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct
    64201 tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc
    64261 tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc
    64321 gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt
    64381 ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc
    64441 tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc
    64501 atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc
    64561 aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta
    64621 cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc
    64681 actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa
    64741 gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt
    64801 aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac
    64861 ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga
    64921 aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg
    64981 tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac
    65041 catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc
    65101 taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat
    65161 agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag
    65221 tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt
    65281 tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg
    65341 tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga
    65401 acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaaggtaac
    65461 ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc
    65521 taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt catcagaggt
    65581 caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc taggaagttc
    65641 gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata aatttagaac
    65701 actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa taacaaaaat
    65761 gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat ttgaacggat
    65821 tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt
    65881 tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg
    65941 gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg
    66001 gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag
    66061 caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag
    66121 gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa
    66181 ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt
    66241 caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat
    66301 accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg
    66361 agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac
    66421 gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg
    66481 tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc
    66541 gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt
    66601 ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata
    66661 gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt
    66721 tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa
    66781 tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat
    66841 attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt
    66901 gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc
    66961 tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac
    67021 ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt
    67081 tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg
    67141 taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc
    67201 agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg
    67261 ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct
    67321 cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca
    67381 aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt
    67441 ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt
    67501 tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt
    67561 ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa
    67621 gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct
    67681 gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga tagcatcaat
    67741 caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa
    67801 ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg
    67861 atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat
    67921 atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac
    67981 cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc
    68041 tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat
    68101 ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac
    68161 attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg
    68221 tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt
    68281 gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac
    68341 gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc
    68401 tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg
    68461 ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac
    68521 gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt
    68581 gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc
    68641 tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc
    68701 caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg
    68761 aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt
    68821 gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact
    68881 tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca
    68941 ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa
    69001 aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa
    69061 gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc
    69121 ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga
    69181 ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc
    69241 cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg
    69301 aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac
    69361 atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg
    69421 gctggaatct gcatttcgat tttattattg atatattttg cttcataatc atagaatgct
    69481 acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca
    69541 acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag
    69601 gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta
    69661 gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca
    69721 aatacttttt taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc
    69781 ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg
    69841 gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt
    69901 ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt
    69961 tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat
    70021 gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat
    70081 tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt
    70141 aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt
    70201 aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat
    70261 attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct
    70321 aaaagaacga acgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa
    70381 agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg
    70441 cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa
    70501 atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac
    70561 agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat
    70621 tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac
    70681 tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt
    70741 ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc
    70801 aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc
    70861 ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag
    70921 taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat
    70981 tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt
    71041 gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga
    71101 acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag
    71161 aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc
    71221 atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac
    71281 aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag
    71341 cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc gattttggta
    71401 tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt
    71461 atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct
    71521 cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt
    71581 ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc
    71641 aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag
    71701 aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg
    71761 ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa agcggattag
    71821 acttattgga agggatgaag gctgcgccta agatccctca acagctccac catctattag
    71881 aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat
    71941 tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga
    72001 tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct
    72061 tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg
    72121 cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca
    72181 gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg
    72241 gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt
    72301 tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc
    72361 ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct
    72421 gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct
    72481 ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta
    72541 tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga
    72601 tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc
    72661 aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg
    72721 tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg
    72781 gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact
    72841 ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata
    72901 aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag
    72961 ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg
    73021 ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac
    73081 actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt
    73141 atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag
    73201 cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag
    73261 tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac
    73321 tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc
    73381 taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat
    73441 tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg
    73501 tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa
    73561 aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat
    73621 caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag
    73681 ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat
    73741 agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa
    73801 tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt
    73861 ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa
    73921 aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc
    73981 agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag
    74041 actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat
    74101 cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac
    74161 cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt
    74221 ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc
    74281 attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa atacagaaag
    74341 aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc
    74401 tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt
    74461 atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg
    74521 cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc
    74581 gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc
    74641 atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct
    74701 ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg
    74761 accacgtact ttatttagca aatataattt acttaaataa atttttccat ttatctcttc
    74821 ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc
    74881 tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg
    74941 tttttgacca atgatcggca caaaaacttc cggccatact tcttgtatga ctgccatcag
    75001 ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca
    75061 ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac
    75121 cgaccacggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa
    75181 accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag
    75241 tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt
    75301 tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat
    75361 tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat
    75421 cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac
    75481 ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa
    75541 agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa
    75601 aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga
    75661 catctatatt ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DACCIQ010000005        64615 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCIQ010000005 DACCIQ010000000
VERSION     DACCIQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 64615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.005"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            31..669
                     /locus_tag="IVM62_000325"
     CDS             31..669
                     /locus_tag="IVM62_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6844320.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(788..860)
                     /locus_tag="IVM62_000326"
     tRNA            complement(788..860)
                     /locus_tag="IVM62_000326"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:Thr,seq:cgt)
     gene            981..1223
                     /locus_tag="IVM62_000327"
     CDS             981..1223
                     /locus_tag="IVM62_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844321.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1362..1991)
                     /gene="upp"
                     /locus_tag="IVM62_000328"
     CDS             complement(1362..1991)
                     /gene="upp"
                     /locus_tag="IVM62_000328"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP6844322.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2125..3072)
                     /locus_tag="IVM62_000329"
     CDS             complement(2125..3072)
                     /locus_tag="IVM62_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6844323.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3225..4469)
                     /locus_tag="IVM62_000330"
     CDS             complement(3225..4469)
                     /locus_tag="IVM62_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP6844324.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4644..5669)
                     /locus_tag="IVM62_000331"
     CDS             complement(4644..5669)
                     /locus_tag="IVM62_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP6844325.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5691..5870)
                     /locus_tag="IVM62_000332"
     CDS             complement(5691..5870)
                     /locus_tag="IVM62_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844326.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5863..6702)
                     /gene="prmC"
                     /locus_tag="IVM62_000333"
     CDS             complement(5863..6702)
                     /gene="prmC"
                     /locus_tag="IVM62_000333"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP6844327.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6695..7768)
                     /gene="prfA"
                     /locus_tag="IVM62_000334"
     CDS             complement(6695..7768)
                     /gene="prfA"
                     /locus_tag="IVM62_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP6844328.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7771..8355)
                     /locus_tag="IVM62_000335"
     CDS             complement(7771..8355)
                     /locus_tag="IVM62_000335"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6844329.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8461..8930)
                     /locus_tag="IVM62_000336"
                     /pseudo
     CDS             complement(8461..8930)
                     /locus_tag="IVM62_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9036..9638
                     /locus_tag="IVM62_000337"
     CDS             9036..9638
                     /locus_tag="IVM62_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6844330.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9742..10311)
                     /gene="lepB"
                     /locus_tag="IVM62_000338"
     CDS             complement(9742..10311)
                     /gene="lepB"
                     /locus_tag="IVM62_000338"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6844331.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10541..11308)
                     /locus_tag="IVM62_000339"
     CDS             complement(10541..11308)
                     /locus_tag="IVM62_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844332.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11305..11784)
                     /locus_tag="IVM62_000340"
     CDS             complement(11305..11784)
                     /locus_tag="IVM62_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP6844333.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11909..12646)
                     /locus_tag="IVM62_000341"
     CDS             complement(11909..12646)
                     /locus_tag="IVM62_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP6844334.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12807..14207
                     /locus_tag="IVM62_000342"
     CDS             12807..14207
                     /locus_tag="IVM62_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP6844335.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14430..15782
                     /locus_tag="IVM62_000343"
     CDS             14430..15782
                     /locus_tag="IVM62_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP6844336.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15775..16473
                     /locus_tag="IVM62_000344"
     CDS             15775..16473
                     /locus_tag="IVM62_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP6844337.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16535..17686)
                     /locus_tag="IVM62_000345"
     CDS             complement(16535..17686)
                     /locus_tag="IVM62_000345"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP6844338.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17699..18136)
                     /locus_tag="IVM62_000346"
     CDS             complement(17699..18136)
                     /locus_tag="IVM62_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6844339.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18151..19917)
                     /locus_tag="IVM62_000347"
     CDS             complement(18151..19917)
                     /locus_tag="IVM62_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP6844340.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20181..20858
                     /locus_tag="IVM62_000348"
     CDS             20181..20858
                     /locus_tag="IVM62_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844341.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20861..21988
                     /locus_tag="IVM62_000349"
     CDS             20861..21988
                     /locus_tag="IVM62_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844342.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22188..22628)
                     /locus_tag="IVM62_000350"
     CDS             complement(22188..22628)
                     /locus_tag="IVM62_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6844343.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22639..24672)
                     /locus_tag="IVM62_000351"
     CDS             complement(22639..24672)
                     /locus_tag="IVM62_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6844344.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24674..26377)
                     /locus_tag="IVM62_000352"
     CDS             complement(24674..26377)
                     /locus_tag="IVM62_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6844345.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26471..27421)
                     /gene="manA"
                     /locus_tag="IVM62_000353"
     CDS             complement(26471..27421)
                     /gene="manA"
                     /locus_tag="IVM62_000353"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP6844346.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27460..28485)
                     /locus_tag="IVM62_000354"
     CDS             complement(27460..28485)
                     /locus_tag="IVM62_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6844347.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28694..29563)
                     /locus_tag="IVM62_000355"
     CDS             complement(28694..29563)
                     /locus_tag="IVM62_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP6844348.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(29703..29909)
                     /locus_tag="IVM62_000356"
     CDS             complement(29703..29909)
                     /locus_tag="IVM62_000356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844349.1"
                     /translation="MQKCRKPFSQNFSYYSKLSDFFTTSSLNGVQKLASAVISPTKRK
                     IGGAIFVWLFLLLDAEQLFHNLFL"
     gene            complement(30267..32159)
                     /locus_tag="IVM62_000357"
     CDS             complement(30267..32159)
                     /locus_tag="IVM62_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844350.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32156..33883)
                     /locus_tag="IVM62_000358"
     CDS             complement(32156..33883)
                     /locus_tag="IVM62_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844351.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33899..34549)
                     /locus_tag="IVM62_000359"
     CDS             complement(33899..34549)
                     /locus_tag="IVM62_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6844352.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34753..35049
                     /locus_tag="IVM62_000360"
     CDS             34753..35049
                     /locus_tag="IVM62_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6844353.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35241..35861
                     /locus_tag="IVM62_000361"
     CDS             35241..35861
                     /locus_tag="IVM62_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP6844354.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35953..37119)
                     /locus_tag="IVM62_000362"
     CDS             complement(35953..37119)
                     /locus_tag="IVM62_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6844355.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37131..37484)
                     /locus_tag="IVM62_000363"
     CDS             complement(37131..37484)
                     /locus_tag="IVM62_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6844356.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37612..38634
                     /locus_tag="IVM62_000364"
     CDS             37612..38634
                     /locus_tag="IVM62_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP6844357.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(39073..39507)
                     /locus_tag="IVM62_000365"
     CDS             complement(39073..39507)
                     /locus_tag="IVM62_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844358.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39617..41476)
                     /locus_tag="IVM62_000366"
     CDS             complement(39617..41476)
                     /locus_tag="IVM62_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6844359.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(41594..42754)
                     /locus_tag="IVM62_000367"
     CDS             complement(41594..42754)
                     /locus_tag="IVM62_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP6844360.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43061..44485)
                     /gene="araA"
                     /locus_tag="IVM62_000368"
     CDS             complement(43061..44485)
                     /gene="araA"
                     /locus_tag="IVM62_000368"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP6844361.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44501..45199)
                     /locus_tag="IVM62_000369"
     CDS             complement(44501..45199)
                     /locus_tag="IVM62_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6844362.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45156..46817)
                     /locus_tag="IVM62_000370"
     CDS             complement(45156..46817)
                     /locus_tag="IVM62_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP6844363.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47035..48435
                     /locus_tag="IVM62_000371"
     CDS             47035..48435
                     /locus_tag="IVM62_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP6844364.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48687..49766)
                     /locus_tag="IVM62_000372"
     CDS             complement(48687..49766)
                     /locus_tag="IVM62_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6844365.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49852..50811)
                     /locus_tag="IVM62_000373"
     CDS             complement(49852..50811)
                     /locus_tag="IVM62_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP6844366.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50823..52346)
                     /locus_tag="IVM62_000374"
     CDS             complement(50823..52346)
                     /locus_tag="IVM62_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6844367.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52356..52919)
                     /locus_tag="IVM62_000375"
     CDS             complement(52356..52919)
                     /locus_tag="IVM62_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6844368.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(52925..53788)
                     /locus_tag="IVM62_000376"
     CDS             complement(52925..53788)
                     /locus_tag="IVM62_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6844369.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53802..54698)
                     /locus_tag="IVM62_000377"
     CDS             complement(53802..54698)
                     /locus_tag="IVM62_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6844370.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54873..56144)
                     /locus_tag="IVM62_000378"
     CDS             complement(54873..56144)
                     /locus_tag="IVM62_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6844371.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56134..57651)
                     /locus_tag="IVM62_000379"
     CDS             complement(56134..57651)
                     /locus_tag="IVM62_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6844372.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57849..58739
                     /locus_tag="IVM62_000380"
     CDS             57849..58739
                     /locus_tag="IVM62_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP6844373.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58812..59636)
                     /gene="nadE"
                     /locus_tag="IVM62_000381"
     CDS             complement(58812..59636)
                     /gene="nadE"
                     /locus_tag="IVM62_000381"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP6844374.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59648..61120)
                     /locus_tag="IVM62_000382"
     CDS             complement(59648..61120)
                     /locus_tag="IVM62_000382"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP6844375.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61757..62119)
                     /locus_tag="IVM62_000383"
     CDS             complement(61757..62119)
                     /locus_tag="IVM62_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6844376.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62342..63094
                     /locus_tag="IVM62_000384"
     CDS             62342..63094
                     /locus_tag="IVM62_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6844377.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63234..64157)
                     /locus_tag="IVM62_000385"
     CDS             complement(63234..64157)
                     /locus_tag="IVM62_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6844378.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64172..64444)
                     /locus_tag="IVM62_000386"
     CDS             complement(64172..64444)
                     /locus_tag="IVM62_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844379.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18618 a        13300 c        11273 g        21424 t
ORIGIN      
        1 ttcaaaaact gaatagtagg agttattatc atgaataaca ttttaggaaa aaaaatagaa
       61 cttaaaaaag ggatattggg cactattgtc gttgtactcg gtttgttagg gttgctattt
      121 gcgccatcca ttattagcat tttttcttta actatcactg gatttatagc atttacactt
      181 gtttttggta aaaaagatac gaaaaaaatg ttctcgaaac ctgtcgctcc tgtcaaaaat
      241 atagccaaat atttttttat caatgtagta atcagtgccg cagtctcggt atttttgcaa
      301 caaattttga aatggggttt aacaggcaat ccaataaatg aggctttcag cccgttatta
      361 tttattattt tgcctatcat gatacttgga gaagaattgt tctcagtgta ttttctcgct
      421 attttttcta gtaagttcag cattcctgtc gctagtttct tgtcagcaat catctttgga
      481 tttatccatt acagtactta tgataatgga aatattttac acactgtagc acacatcctt
      541 ttgatacaag gggtggcaag attgttattc aaccaagctg ccattaaaag caactcgatc
      601 attacaagtt gggcagttca tgtgatattt gatttcacag ctatattgct agtcgtttta
      661 ttctcctaaa tttttataga aataatacat gctctattgc taagaaagca tactttattt
      721 cctttaaaca aaaagacaga tgtatcaatg gattaaaaat ccttgatata tctgtcttct
      781 cttttaatgc cagctatagg gatcgaacct acgacctacg cgttacgagt gcgttgctct
      841 accaactgag ctaagctggc cttatccaaa agaaattata tactctattt ggctgattgt
      901 aaagagtccg atggaaaatt agtttaattt catgatattt ttcataaaac gtggtataat
      961 tgatgtgaaa ggcaggtttt atgaaaatgg caacttttgg aaaatttgat acaactatcg
     1021 taccatcgga aattggtgaa aaattcaaga tcaatactca cgtaatgttc acaattcacg
     1081 gcaagtcgca aactggaacg atttcaaagc aattgaaaaa ttccgctgtt gttgagattg
     1141 atgaaactgc agaaaatgcg aacttgatga cgcaaagtaa gggtgtagta atcgttaact
     1201 acaaagaact aaaaaagatt tgaatttgac catctctgca tttttgggat ggttttttta
     1261 tatgcaaacg aaagctgtga aaaagctgtt ctgcatcaaa cgttaaaaat gaccaaaaaa
     1321 ggatgtgaca aaagttgtgt cacacccttt ttcgtattta cttattttgt accaaacaaa
     1381 cggtcacccg catcccctag tccaggaacg atataaccat gttcattcaa tttttcatct
     1441 agagaagctg tatagatgtc aatatcagga tgcgcttctt gcaaagcttt tactccctca
     1501 ggtgctgcta ccaaacaaac aaatttaatg ttcgacgcgc cacgttcttt caatgcatcg
     1561 attgccatga ttgctgatcc gcctgttgcc aacattggat ctactacaaa caactgacgt
     1621 gcatcaatgt cttctggaag tttgacgaaa tattcatgtg gttccaatgt ttcttcatct
     1681 cgataaagac cgatatgtcc cactttagca gctggaatca attccaagat accgtcaacc
     1741 attccaatac ctgcgcgcaa gattggaata atcgctactt ttttaccaga caacgttttt
     1801 tgtgttgttt cagtgatcgg tgtttcgatc acgatgtctt ccaatggcat atcccgtgat
     1861 acttcatatg ccatcaacat agcgatttca tcgactactt cacgaaaaac ctttgttccg
     1921 cagtttttgt cccgaataat agttagtttg tgttgaatca atggatgatc gatcacttga
     1981 aatttgccca ttttttaagc tctcctttgt tctgtcgttt ctttttaatt gtaaaacaaa
     2041 atcaagtgat tgactagtca aactcgccat ttttcctaag aaaatccgtt tttgaaaaga
     2101 agttttcctg cggacaagca aagcttattc ttttttcttg atccaagaag tagataatgc
     2161 cattgctgca cgcgttgcct ttgtctgatc catagcattt accatgacaa aatcatggat
     2221 gattccttgg aagcgtactt gtgtaacagg aacccctgca tcacgaagtt tgcgcgcata
     2281 agcctcacct tcatctctta atacatccgc ttctcccgtt aaaatcattg ctgaaggaag
     2341 atcatttagt tcatttatat ttgctctcaa aggcgaagct gtgatttcag cacgctcttc
     2401 ttcattcgtc gtatattggt cccaaaacca catcattcct tctcttgtca aaaaatagtt
     2461 ttctccgaat tcatgatagg aatctgtatc aaaatcagcg tttgtgactg gataatacag
     2521 caattgcttg gatattggga ggccctttcg ttcttttgtt aagatcgtca tgactgtagc
     2581 catgttacca cctactgaat ctcctgcaac gattagattc tcgagatcca gattttttga
     2641 cttggctaat ttattcagct gttgcaacac agcataattt tgttcaattg ctgttggata
     2701 ttttgcttct ggtgatcgac tatattctgg aaagacaacg acggaattcg tacgaaccgc
     2761 aagttctctg atcaatttat catgggtttg agcactgcca aaaacccagc ctgctccatg
     2821 aatataaaaa ataactggta atttatccat attcccttct ggtcgtacga gacgaatatt
     2881 gagttttccc cattcgccag tatccaccat caagtcttca atatcgacgg aatatttgtt
     2941 cacaggtgat tcttgtactt cttctagtaa aactcttcct tcttccggtg ctagttcata
     3001 gatacgcgga tgaggcgcat ttgcatcact gaatgctact gcttcctttt ctaacgagat
     3061 acgatcagtc atgacttttc ctccctgaga tttagtgtga agactaaaat tacacctcat
     3121 atactagaga gcatcaccga tattgtcaaa gaatccgttt tcccaaaaaa taaacagaaa
     3181 agcccaacat tgaacttttc tgtttactaa tggaatttaa atttttattt tttatgcaac
     3241 ggaaattttt cagttaattc gcgtacacct gttttgactt cgtctagttg agcgttatca
     3301 tcttttgctt gtaatgcttt gacaactagt tcagccactt ttacagcatc ttcttctttg
     3361 aacccgcgag tagtgattgc tggcgtgccg attcgaatac cgcttgtttt gaatggactt
     3421 agtgtttcaa aaggaatcga gtttttattt acagtgatat tcacactgtc caaaatgctc
     3481 tctgcttctt ttccatttat acctaactcg cgaacatcga tcaacatcag atgattatct
     3541 gtcgcccctg aaatgactct ggttccaatt gcttgattga aaactttgac cattgctttt
     3601 gcattggcga tgatttgttc actatattct ttaaatgcag gatctaatgc ttctttgaat
     3661 gctactgctt tacccgcaat cacatgctct agagggccgc cttgaatacc aggaaatacc
     3721 gcactattta ttttttttgc taatgcttca tcgtttgtca aaatcatgcc gcctcgaggg
     3781 ccacgcaatg ttttatgagt ggtagtcgtt gtgatatctg cataaggaac tggattagga
     3841 tgaagtcctg ctgctactaa tcctgcgata tgtgccatat ctaccatcag ttttgcgcct
     3901 acttcatctg cgatttctct gaatttcgca aagtcgatcg ttcgtccgta agcactggca
     3961 cctgctacga tcaattttgg ttgatgtttt ctagctaaaa tacgtaccac attataatca
     4021 ataacttctg ttgtcggatc aacaccgtaa gcaacaaagt gataggtttt gccactgaag
     4081 ttgactggtg atccatgtgt tagatgtccg cctgcggaaa gatccattcc taagatcgtg
     4141 tcccctggtt ctaccaaagc gagataagca gccgtgttgg cttgagagcc ggagtgaggc
     4201 tggacgttcg caaatttcgc tccgaataat tcttttgcac ggtcaatcgc taaattttca
     4261 acgatatcga caaattcaca cccaccataa tagcgatgcc ctggataacc ttctgcgtat
     4321 ttatttgtca agatacttcc ttgtgcagcc atcactgctt cggagacaaa gttttcagag
     4381 gcaatcagct ccaaattatg ttcttgtctt tcttcttctt tggcgatcgc agcccaaaga
     4441 tcagggtcaa acgttttgta atctaccaca aacaacagct cctttttatt ttcctacaat
     4501 tttatttttc ccatcaaaaa caaattgcca aatattcgta tctcttctat tacgattagt
     4561 tacgcttagt ttttcgttca ctgatagaag atttacttga tagcattgta tcatactaaa
     4621 agaatgcgac aataaccaat tgatcatttt tcaaaataat tttgtcctgc tgcttttttc
     4681 aagcgattca tgtaagcatt ccccaatcca gtttctggat acacttgtac aaaaatcaaa
     4741 tctaatccaa gtgttggttc atctaatcct cttagacctg caaacaatcc tttagccgcc
     4801 gcttctactg agttgtcgtt atacatataa acagctgcag tatctgtccg cacttgatcc
     4861 gcaatttcgg ggcttgctat gacacctgca cggatcttct tattttttgc ccattgaact
     4921 gcagttgacc aatcgccatc tctgaccatc agaacacgtg tatcgggtga gtagtgtttg
     4981 tatttcatcc caggggcttt tggtgtttca ttttctttta caagatgctg atcaatagca
     5041 acaggagatt cgatgactgc ttcgatttgt tctttcgtta ctgcacctgg tcgtaaaatc
     5101 ataggcatgg ctgttggatc acttaaatca agaacagtgg attctacacc aattcgcgta
     5161 gctccatcat caataatccc ggtaattttt ccttgtagat catgatatac atgatctgca
     5221 gtcgttggac ttggttttcc cgacgtattt gcacttggac cgacaagcgg aacaccggaa
     5281 agttcgatta cttctagtgt ttttttgtta tccggtatac gaaatgcagc tgtggataat
     5341 cctcccgtca caactgaggg caaagtgtct tttttgatct taaatatcaa agttaatggt
     5401 ccaggccaga agttcttcac aatcttctct gctgccggat gaaagttgtc cacatactct
     5461 tttacttgtt caaaagaggc gacatggacg attaatggat tatcttgcgg tctccctttt
     5521 acagaaaaca ctttcttgac agcgtttggt aaaagagcgt tggcgcccaa tccgtatact
     5581 gtttcggtag gaaaagcaac aagttctcct tctttcagtg cttcggctgc ctcttgcaat
     5641 tctgtttcat catatcgttt cgtttccaca gtttatccac aacccctttt tcatacattc
     5701 ttattttgtc tgagtttatc ctcaagtttt ccactgtttg tggataatct atttgtcaat
     5761 agtggataaa cttgttgata aaccaaaaag tcgtttacat ccttgtcaaa taagtgtttt
     5821 tttataataa aaaaacaaat aaaaaatttt tcatccacaa cttcaaccat aaacttggat
     5881 cattcgttca ttgccaaaaa gatccttttt tatcgccact tttttatctg gaaatgcttg
     5941 ctgaaatatt ttttttactg cttcaccttg gcgaaatccg atttctaaaa aaatctttcc
     6001 atttgatttt agtacgcttg gtgcttcaac tgctattttt tcataaatag ctaatccatc
     6061 gttctcagca aaaagggcca ttttaggttc gaatgaccgg acactttcat ccatcagttc
     6121 ccattcattc cgactgatgt acggcggatt agaaataatc acatcaaatt gctgatccat
     6181 tactggtcct aatgtatctc catgataaaa actgattttc gttccttctt tctgagcatt
     6241 ttctttcgct acttctaatg cttcttctga aagatccaca gcagatatat gccaattttt
     6301 tcttgctgct ttcaaactaa tagcaattgc tccagtccct gtcccaatat ctatcacttc
     6361 taacggtaca ccaggcgtct cgtctaaaca ccattcgact aactcctctg tttctggtcg
     6421 agggatcagt gttgcttcag ttacctttaa ccgatgatca taaaaatctg catatcctag
     6481 caaatactga ggtggatgat ctgcaagcag aagtcgcaga tcggtttcga tcaaccgctg
     6541 ttcttcttct gaaatctctt cgttcatatg aaggagccaa tcaagttttt tccattgttt
     6601 tcgttccaaa aataaaaatt ggatactata tgcttctttc ccatgatcct ctaaaaaaga
     6661 agaagcccgt gacaggactt ctcgataggt cattttaccc attttgcata gcttccagtt
     6721 ttgatgtttg atcatacatg atcaatgcat caacgatctc atctaatttc cctgctaaaa
     6781 tctgatctaa tttttggata gttaatccga tacgatgatc agtcacacga ttttgcggga
     6841 aattatacgt ccggatccgt tctgaacgat ccccagttcc gactgctgat tttcgattgg
     6901 catcatactc gctttgtgct tcttgctgga tcttatcgta cacacgagca cgcaagattt
     6961 tcattgcttt ttcacggttc ttgatttgag aacgttcatc ttgcatagca acaacgatcc
     7021 ctgttggtaa gtgagtcaaa cgtacagcag atgctgtttt attgacgtgc tgtccgccag
     7081 cacctgaagc atgatagata tctgtacgga tatctttgtc ttcaatatcg atctctactt
     7141 cttcagcttc tggcatcacg acaactgttg cggtggaagt atggatacgc ccttgcgatt
     7201 cagttgaagg aacacgctgg acgcggtgcg caccactttc atattttaat ttcgaatata
     7261 cgttgtctcc ggtgatcatc atgatcactt ctttatatcc accgattccg gtaacgttag
     7321 cttccatcac ttctgttttc cagccttgtg cttcagcata cttttggtac atattgaaaa
     7381 gatcgccagc gaataatgct gcttcgtctc cgcctgctgc tccacggatt tccatgatga
     7441 tatttttatc gtcatttgga tcttttggta aaagcaagat cttgattttt tcttctaaga
     7501 cttctttttc tttttttaat tcagataatt cttcttttgc tagttcagcc atttcatcat
     7561 ccagtttttc accaagcagt tcttctgtat cggtgattcc ttgtactact tctttataac
     7621 gacgataaac ttcgactgtt tcgcgcgtgt ttgcttcttc ttttgaaagc tgcataaaac
     7681 gctttgtatc agaaatcact tctgggtcac ttaaaagttc tccaagctct tcataacggt
     7741 cttcaattgc ttgtaattga tcgtacatgc ttattctcct tctgtaatca ttgatgggtg
     7801 aaagtaatgt tttcgacaaa cggggtaata agcttcattt ccgccaattt gcacttgatc
     7861 gccttcataa acagggtttc catcaatata atgtaaattc atgattgctt ttttgtggca
     7921 gaaccaacag attgttttca tttcttcgat tttgtctgca taaagcaata aatattttga
     7981 tccttcaaat aactcgtttc gaaaatcatt tttcaaacca aatgccataa ccggaatatc
     8041 caattcgtcc acaattctcg taaaagcaat gacatgtttc ttttttaaga attgcgcttc
     8101 atcgatcagc acacaatagg gtttgctttc cattttctga attacgtcaa atacgtctgt
     8161 ttcttcaaat atcggcatcg cattacgttt cagtccgatt cggcttgaaa caacgcctaa
     8221 accatctctt gtatcaatac cgcttgtcat taagacaact ggcttatttt gttcttcata
     8281 attatgtgcg acttttagga tctcgatcgt cttgccgcta ttcattgcgc catacttaaa
     8341 aaatagttgt gccatgctct ttttcacctt ccagtttgtt cctatggtat tatacttgat
     8401 tttcagcatt ttttacagtg agaattttca tcaagcacag attagtatta aatctattgc
     8461 tcacttttgt tccaattttt tgacaagaac taaacatggg ttccattcat cccataaatt
     8521 tggaaaaact tccaaccttg aaaatcctaa tgattcgtag aaacaaatag tttgatcata
     8581 tatagagtag tgcccctcgt ctacagtctt cacttgcaaa tatttgtaat gttttgctgc
     8641 gtaaccttct gcttctttaa aaagttttgt tcctaatcct tggtgatgat agtctttttt
     8701 tattcccata caatagaata gatttcggca gtatcttcac ttgtctgtga aagagtgata
     8761 aagcctaaat attcttcttt tcttttttca caccataaag gtagtttact tgattcctcg
     8821 atatattttt ccgttgattc aggaagccca aaccactcag gcaagtctag taatatttct
     8881 cggacacact ttcctttttc ctctctatct tttacaaacg atatcggcat attctttcta
     8941 gattctccct ttttgaattg agtatagcat gctttctttc ttttcacgtg ctataataag
     9001 ttaaaatttc aaagttttac gtaaggagct atttgatgac aaaaaatatt tttgattcca
     9061 tcgctaacca ttatgattcc cctgaccgtc aagcattagc caagattatt cgcgaagaac
     9121 taactactta tctaccgcgc gacagccacc aaaaagtcct gctggattac ggaggcggaa
     9181 ctgggcttgt tagtttaccg cttgcggaaa gattcaaaga actgatcatt gccgatgctt
     9241 cagaaactat gctgaaaatg gcagaagaaa agatccaagc agcagatctg aaaaatgtcc
     9301 gaacgatcca cgctgatgct tctgtcgaat ttcctgctgt ccaagctaat ctcatccttc
     9361 tatcacttgt acttttacac attcctgata cggagaatat tcttacaaaa ctttatgaaa
     9421 ttttagctcc tggcggtcaa ttgatcatcg tagactttga caagaatgaa caaattagtc
     9481 atccgaaagt ccataatggc tttagccaag aagaattgaa taatcgattg gaaaaaacgg
     9541 gctttgtctc tgctactagt cataccttcc atcgtgggga aaagctattt atgaacaaaa
     9601 acgcttcgct tttcttatcc atcagccaaa aagcttaaaa tgagattgca aaaaaagtga
     9661 gcaactccaa gaaataagaa ggaattttcg gaaaaagaag ctgtgacaaa aaacatgtca
     9721 cagcttcttt ttattttttc atcacatgat tttaatttct gaaagaggcc aataacggaa
     9781 aacaactttt ccttggatca tatctttgtc gactaaccca aacattcggc tgtcttttga
     9841 aatcaagcgg ttatcgccta gaacaagata ctttcctttt ggcacagtat attcaaagtc
     9901 actcgtaaag gtggatgaac tttctgcttg tgcttggaat cctgagctat aagcatattc
     9961 tccttgtaac ttatcttctg aaaattcttt tttgaactca tccagataag gttcatcata
    10021 ctttttgcca ttaatggtca actggtcatt ttccatcttg actgtatctc caggcatacc
    10081 gatgatccgt ttaacaatca tccgttcttc atcccccggt tcctttgtag taataatatc
    10141 cattcgttca tagtttgaga tcttagatga aatcaatcgt tgtccatcac ttaatgtagg
    10201 gtccatcgaa tgcccgttta cagtaactgg tgtaaacacg tacactctag ctaataaaat
    10261 agccgctaat acaaggatca aaatccccca atttttcaaa aattctttca cagcttacct
    10321 cctacttgta ttttcctcgt tatttattac gttttttcac aatacttcgt tgtactttct
    10381 ttattctaca atgtttataa atgaaaggaa agaataagtg atgtgttcgt cttatctttc
    10441 tcgttatata aaaaaggaac gtgacaaaag acgtgtcaca tcccccttat tccgaataaa
    10501 cgtttttatc gcaaccactg atacacggcg tttcatcaca ttatgaatgt cttcttcttt
    10561 ggctacgact ataaaccact ggttcttcct gcgactgtgc cttttttgca atcaatttca
    10621 gacgataagg ctcatgatag cgtaacccgt ctttgacaaa atcaccgccg acgatgaaca
    10681 atccgacagg ttccaatgtg tcttctgttg agtcaagtcg aatggtccct actttttcca
    10741 tgtccgagta gcttcgtcca atatttagaa catactcttt ctctcctgtt ttcttgacat
    10801 agtaataagg atacaatgta tttctatcga ccagaaatcc atccatgcga ccgagttcat
    10861 ttgttttgat ctcaccattt ccaatcaaat accgcatatt ctcggctaat ttcaattgat
    10921 aatcttcgtc ataagaaacc atgacctctt gtgtattcac tttatcgatc gaatttccat
    10981 tatctatcga ttcttctgtc tggatgttga acgcttcatc aggtaaatac ttcacaaaaa
    11041 gctgagtgac atcggctttc gtgtttttat ctaaaaagta ataaaataca tttaaaacag
    11101 taaaataaac aagcactaga aatgcaagtc catataagaa aaattgaaga tagttttggt
    11161 tttgccaaag tccataaaca attctcaata aataaagcgt gggaatcaca cttaaaatcg
    11221 tatatccacg gttaagatat ttaggactga tgtctaaata gccaaggaat ttatgcaccc
    11281 cgttgataag gtctagtaaa aatgtcattg agaatcacct gcttgctggt tatacgtttg
    11341 ggtatttcct gaatcttggt tttgctgtgc tgtacgtgct gcatcatcat tttgcgtgtt
    11401 atcatctaca gtcgtactgc ttggtatcgt ttcggagcta gcacttgtat ccggacttgt
    11461 acttggattg gttgaatccg atggagtgcc atctggtacc gtattacttg tttcgcttga
    11521 gctgtcagtt gtatatgtct catcatacga cgaactagtt gttgtatcgt cccatgtact
    11581 tgtcgtagtg gtttcattca aactttccgt tgttgttggt tcagatgtcg agactgaagt
    11641 agttggtgtt gtttcttcta cagtactaga acttgaactt ggtgcacggt agctggaact
    11701 attcacaata cctgtagtag tcgcgacaat cgtcgagatt gttaatacag cgattggctt
    11761 caaaataggt gctacttttc gcatcttatc tcttccttca ttgaaattta ttcttgtttc
    11821 cttactttac tacggacaaa ttatacagtc taccttattt ctaccataaa ttctttattt
    11881 ttttcttacg tttttccgaa agatttcttt aattttcctc aaaaactacg gttgatgttt
    11941 ttaagtaagc ttttgcttcc cgattgattt ctttcagatc gcatccttgc tgcttggcag
    12001 cgaacacgca accacaataa cattggcgat aaatatcata ctctttgcac atttgcaaag
    12061 agcgttcata gcctttgttt ttcttgaaat cacttggcaa gtattgtgtc ccgtaaatct
    12121 tttggatatc cattccgatc tgattgatca aagctgcatt tttctttggc gagatggtca
    12181 gggcacttcc aaagtaatcg tatcctagtt cttgcgcttt ttgcgcaaca agatctaatc
    12241 gcatattgaa gcatgcactg cagcgagcac caccttcttt ttcgttttca agatggtttg
    12301 ttaaaaccat tttggtgaat tcatttggac ggtattcatc aacgattagc gcaacatgat
    12361 ggtcagtttt ctcattaaat tggtctacaa atcgtttttg ttcatgtaat cttcgcaaat
    12421 actcactttt aggatgaata ttcgaatttg aaaaaaataa cgtgatctct gcatattgag
    12481 acatgaattc caatgtgtgc gtactgcaag gagcacagca tgtatgtatt aatatcttag
    12541 gctttacttc ttctttctcc cattgcatga tcattttttt cagaactgta tcataattta
    12601 ttttttgatt tttcattttt tctacgattt gttgagcatt aatcaatttg ttattcctcc
    12661 tccttctttt ttctaggatt tgccttatta taagccattt ctattttaat cgaaagctgt
    12721 attgttatct gtttgataat ttctttgagt attcctaatt ccccttgtat actggtagaa
    12781 acgactattg tcaggaggat agtactgtgc atgttgattt attctttcga aggctgcagc
    12841 ggcgaggaca gaagtttgct gccaatcatt tctcatcgat ccaaatcatc gttttttatt
    12901 atatattgat gacggtctta tcactcgttc tattttatat gccgattttt cgcgaaccag
    12961 atagccatgt ttcttttgtg gacatgctgt tcatggcaat cagtacagtg agtgtgactg
    13021 gactatcgac ttttgatatc aattctgtgt tcaatgataa cggtattata ctgctggaaa
    13081 tattatttca agttggtggt ttggggatca tgatgatttc tactgctttt gtcatttttt
    13141 ctaaacgacg gatcacactg aaacagcgac agctgatcat gacggatatg aatcaaccgc
    13201 gtttgtctgg tatcgtccga ctgattcgga tcacttttgc tattttaatc tggtttcaat
    13261 tactttttgg gacattcttt tctatttatt tttattacag aggctatttt gatcgttggc
    13321 gtgatgccgt tttttacggt ttttatcagg cgatatcggc tgtcacgaac tctgggtttg
    13381 acgtcactgg ggattccatc aagccctttg cacatgacta ttttttcttg atcctgatca
    13441 tgtttttgat ttttgtcgga ggaattggtt ttcctgtatt gatggagtgc cgggaatggc
    13501 tattatacaa acgttccaat gcgaagcttc cgtttcgttt ttccttgttt accaaattag
    13561 ctgtgttagc ttttgttatc ttgtttgttt cgggaactgt cttgatttat ctattggaaa
    13621 aggaccattt attccaagat tccaatatga gtgtcaaatg gatcaattcg atgttttatt
    13681 cgattacgac tcgtaatgct ggtctgcaga ttcacgattt gggtgatttt caaatcacaa
    13741 cactgatcat tttttcttta ctgatgttta tcggatgcag tcctagttct gtaggcggcg
    13801 ggattcgaac aactactgta gcaatcatcg gtttgtatct atattccttc ctcaaaagtg
    13861 aagataacat caatattttt ggacggagaa tcgatcaaga tgatgtgcga aaatcagtcg
    13921 ttgtctttat gctttctctt ggtatgtgtt tcttttgtat cgttttttta tctgctacag
    13981 aagaacaaac gctgatctcg atcatcatcg aagtaacttc tgcgtttgga acaactggtt
    14041 tatctttagg gattaccgga gacttgtctg ttgtgggcaa aatcacgatt gctacactga
    14101 tgtttattgg gcggatcgga atgctatata cattgatgtt attcgtgcca aaagaaacac
    14161 gagatctagg ctatgaatat ccaagcgaga aaattattat cggttaaaat gggaatatag
    14221 gtaagaggga ataataaaat cacagcagct tgaaacaatc attttgtttc aagctgtttt
    14281 tttgttaaat catgcgattt tgtccgattt catgcacttt cagccgtaac cttttcttaa
    14341 gaataagaaa agcaatttaa cctttttttc aacatatggt atgattagtt gaatttattc
    14401 gtcagcaaat cgtttttgga ggttacataa tgggaatacg cagtcatttt gcgattgcag
    14461 ttggaaaaac gtcgcaatgg gcattaaaaa ctttttttaa aggcggatca agtttaccag
    14521 gtaaactagc gctgaaagtc gatccacata tactagatag tttggcaaaa gactaccaag
    14581 tcgtcgttgt caccggaaca aacggtaaaa cattgactac cgctttaaca gtcaatattt
    14641 tgcgccagca attcgatgaa gttctaacca accctactgg tgcgaatatg gtacagggaa
    14701 ttgtatcgac tttcttgcag gcaaaaccaa aaaaaggaca gaaaaagttt gctgtcctag
    14761 aaatagatga agcaagtttg agcaaagtca cagaatatat gaaaccagaa ctgttcttat
    14821 ttacaaatat tttccgtgat caaatggatc ggtatggaga aatctatacg acctataaaa
    14881 tgattgtaga cggtgccgca aagtcaccaa atgcaacgat tatcagtaac ggtgattcac
    14941 caatcttcaa ttctatcgaa acagtcaatc ctagaaaata ttatggcttt gaccatgaag
    15001 aagatcgtga acaaatggct cattacaata cagacggtgt cttatgtcca aattgtcatc
    15061 acatcttgca ttataaaatg atcacttatg caaacttagg gaaatattac tgtccaaatt
    15121 gtgacttcaa acgtcctgaa ttggattatc gattaactga tatggtacgg atggacaatg
    15181 tgtcagcaga tttcgtgatc gatggtcatg aatacggaat cgaagttggc ggtatgtata
    15241 atgtctacaa tgctttagca gctactgcag tggcagagta cttcaatgtc gatcctgata
    15301 aaatcaaaca aggtttagga tatgacgaga aagtattcgg ccgacaagaa gtcatcaata
    15361 tcaatgggaa aaaatgtaca ttagtacttg tcaaaaatcc tgtaggtatc aatcaagtaa
    15421 tcgatatgat tggtctagct ccttatccat tttctttagt ggctttatta aatgccaatt
    15481 acgcagacgg aatcgatgtc agctggattt gggatggtaa ttttgaaaac tttgccgata
    15541 tggatatccc agctgttatt tctggaggag accgtcatac agatatgact ttacgtctaa
    15601 aagttgccgg gattccagaa aataaattga cagaaacaaa agaattacca gaagtaatcg
    15661 aaaaaatcaa agaattgcca actgagcatg tctatatttt agctacttac actgctgtat
    15721 tgcagcttcg taaagaacta gctgctcaag gatttatcaa aggaggaatg aaccgtggct
    15781 aattatgaac tcaatatcgc ccacctttat ggaaatttga tgaacacata tggtgataac
    15841 ggaaaccttt tgatgttgaa atatatagca gaaaaaatgg gggtttcttg ccatacggag
    15901 atcgttagta tacatgaacc ttttgatgct gataaatatg acttggtttt ctttggcggt
    15961 ggtcaagact ttgagcaatt aatcatttca aaagacatcc agaaaaagaa agattctttg
    16021 atcaactata tcgaaaatga tggcgtcatg ttagccattt gtggtggcta ccaattgcta
    16081 ggtcactact acatgggagc taatggagaa aagatccaag gaatcagtgc actggatcac
    16141 tacacgttaa gtcaggaaaa taaccggttt atcggtgata tcgttatcca taacgaagaa
    16201 ttcaatgaaa cttattatgg atttgaaaac cacaatggtc ggactttctt aggtgaaggt
    16261 gaacgacctt tggggaaagt ggtacaaggt caaggaaata atggtgaaga ccaatctgaa
    16321 ggtgtcattt accgaaatgt cttcggttct tatttccatg gacctatcct tgcaagaaat
    16381 gagaacctgg cagctcgttt gatccgccta gctttagaac aacgttacgg taaagaattg
    16441 gatctgccct atcttgaagg acaaacagcc tagttattcg aaaaagggga atgtgacaaa
    16501 aatttcgtca cattcccctt tttcgtatcc tattttaagc gacttgcttg gcataacggt
    16561 tcacgttttg cttgatattc ccgattgttt tcggatcatt gattcctgtg acttcttgaa
    16621 tcaattgttc caagttttca tggatcttca tttcatgcaa ttgacggctt tgttcatctt
    16681 caggatcata gtaattaaaa ataatcccca ttgttgccgt caaatgtggg caagtcaagt
    16741 tatgttcttg tagttctctc actggacgtg taaatctttc ttgataacct aacttacgta
    16801 atggtgtccg agcgacacgt gtgatcgcat cagagatatt tttgttttgg aagcgatgaa
    16861 tgattttttc aatatacgca tgatgctgct ctgcatcaaa cccccatttg gcaatcagca
    16921 aacttcctgt ttcctgcaaa actgatttta attgtgccac aactaacgca tcttgcattg
    16981 cttcatcaat ggtctggtaa cctaacaagg cacctgtata agcgacagtc gcatgaccag
    17041 tattgacact aaatagtttg cgttcgatat atggttctag atcatctaca tataaaaccc
    17101 cttttaattg gattttttta ttttttcttt ggctatcgtc aatgacccat tctttgaaag
    17161 gttccacttg gacgaataaa ggatcttcat ggtgttgtaa tgggacgatt cgatcgactg
    17221 ctgcatttgg aaagccgata tatttgtcag taaatgatgt ttccggaaga tatttataca
    17281 cttctttttc tagaaatgct gaaccgccaa tcatattttc acaggcaatg atatctaaag
    17341 atgttgtgct tccttccttt tctctttctt ggataccttt tgcaatcaac tcagcaataa
    17401 aaggtaagat attcgggcca atggcggtag taactaaatc tgcttcagcg atttcttgga
    17461 ttactttttc tggatttgtt tgattgttga ttcctttgac attttttaca gtgatttttt
    17521 cttttgattc atctgctaat tcgatttcgt attcttttct ttcatttaaa gcatcaatga
    17581 tcgtgtgatt cacatctacg aaagtaatct caaatccatt agctgccaaa acctctccaa
    17641 taaatcctcg accgatattt cctgcaccaa aatgtactgc tctcattttt ctctctcctt
    17701 aagcaattgc tagattttcc gttacttctt ctttgctgag cgcgtctgct agctggacaa
    17761 cattgtccat atcactgcaa taaagtgcga tttgctgtac aagttgcaaa tgctcatctc
    17821 cgactcctgc aataccgaac aaaacagtcg caatcttttc ttcttcttcg gtaccaaaat
    17881 ctacaccatc tggtacttga acgacacaga tgccggattt tttcacatat ttttttgctt
    17941 catctgttcc atgaggaatc gcgataaaat tccccatata aacagataac attttgtctc
    18001 tatctaccat ggcatcgata tattcctcat caacgcaccc tgcttctaca agcatctgac
    18061 cgcaatagcg gatcgcttct tctttggttt taaaagatcg attgagctcg atcatgtcga
    18121 atggcaaact ttgcatgctg ttttcctccc ttaagctgtc tgtttcaaat attgtgcaat
    18181 cgattcgtat ttttgcggat tgaccatgct gttgactgat aaatgaatgg attgaggtgc
    18241 agccatctgt actttttccg ctaaactttc tttggctacg atcagtgaat caggttcatc
    18301 ttttaaatct tggatattga cttttcttaa cgggatagtt aaatggttct tgtctaatat
    18361 atctttgagc acggtcatcc ccattgttgc tgatccggaa cgatcatcgt ggacaagtag
    18421 aatctctttg atttgtttga tgtttgctga aagaaactct tctgctggct cattctcaat
    18481 ttcagaaact gatgttttct gcttgctgct taattcttga atgatttcat catatcgagg
    18541 tgaattcaaa aagttttcta ctgagacaaa acgcgcgctc ggtgcttttt gttttgctct
    18601 ttcttgtagt tcgacctgag taacgatcaa tgtattcgag tcatctgtca gttggctgat
    18661 tgcttgattg gtcaccgttt gaggcaagtc tgcttcctgt actttttttc tcaagatcga
    18721 agcgcccatc gcacttgatc ccattcctgc atcacaagca aaaatgattt tttgtacgcc
    18781 agagaatgta gctccttgag gaataccttg ggcacctttg gaagcttgct tagctgcctt
    18841 tgtctcttga acagattttt cgaagtcttc atttgtatct ttcgcatccg ctttcaaaat
    18901 tactgcagaa ataccaaacg aaacagctgc ggcaacaatc acgcccaaaa taacaggaag
    18961 gtaagaattg aacggtgtca tcgctagaat cgcaatgatc gaccctggag aagctggcgc
    19021 tcttagtcca gcatccatca attggaaaat gaaacttcct gacattcctc cgcctataac
    19081 tgataagaat aataacggtt tcatcataac gtaaggaaaa tagatttcat ggatcccgcc
    19141 taaaaagtga atgatcatgg ctcctggtgc tgaagatttt gctgctcctt tgccaaacaa
    19201 agtaaaagca agtaatacgc caagtcctgg accaggattt gcttcaagca agaatagaat
    19261 agattttcca gcctctgcta cttgttcagt tcccaatggt gtcaaaatcc catgattgat
    19321 tgcattattc aaaaataaga tttttgctgg ttcaataaaa atacttgtca atggaatcaa
    19381 atgggcagat aaaatgatct ctacaccttt tgccattgct tgagtcaatg catcgacaac
    19441 tggaccaatc gcagaaaaac ctaataacgc taatccaaaa ccgataagac ctgctgaaaa
    19501 attattgact agcatttcaa aaccggattt gatccgtttt tggaatagct gatcaaattt
    19561 cttgatggca tatccaccaa gaggtcccat gatcatcgcg cctagcatca ttggaatatc
    19621 tgttccgaca atcacaccca tcgttgcaat tgccccaaca actgatccgc ggtcaccagc
    19681 aattactttt ccgccagtat aaccgatcaa taaaggaatc agataagtta gcattgggct
    19741 taccatggta gcgaaagcct catttggtaa ataaccatct gggataaata gagccgttaa
    19801 tacaccccag gcaatcagtg ctccaatatt tggcatgacc atgcttgata aagtactgcc
    19861 taatttttgt accctcgctt ttagcgaaat gttgtttttg ggaatcgtcc gttccatttt
    19921 gtctctccct ctctcctttt gatactctaa ttgtatccgc ttactaaaaa gagaaatagt
    19981 cgtactttgg cacgaaactt tgtgccaaag gtttgagtat gtcagcaaaa taaaaagatt
    20041 tcggtatcag aaaacttcaa ttttaaataa aactgttatt ttaatttttt tataatatgc
    20101 aaacaataac ttttgttagc atttaattta gatgttttcc attctattat gttattaaat
    20161 tttatattgg aggcctatct atgacattac tgatcaaaaa catgaacatg aaacgtggta
    20221 aaaaacgtgt gattcgacaa ttgaatcttt tagtagaatc aggagaagta gctgctttga
    20281 ttgccccgaa cggatttggc aaaacaactt tgctgaatgg cattgcccaa ctgctgccaa
    20341 caacttatga tcaattgtcc cttcacaaca tctctcctac tgcttataca gattatcgcc
    20401 ggtctttctt ttttctagaa tcaacgaatc agttatccga ctatctatcg gctgttgatt
    20461 atttgacttt gatcaaatac tattggcaga gcaaagtaga atgccaaact gttttccaac
    20521 gattgaagat gactgagtac cttactttgc cgattggaaa aatgtctcta ggaatgaaac
    20581 aacatgtctt gcttgctgct tacttgatca gtgattcatc tgtcatgtta ttcgatgaac
    20641 cgttgaacgg attagaccca ggaagtattg atatcttgaa tagcctcatc agacaatggc
    20701 agaaagaggg gaagacgatt cttctttctt cccacgctat cgcgaatatc caatcgattt
    20761 gttctaaagc atgttttcta aaaaatggag aaatcgtcac agaatcctca aatatggaag
    20821 agattttagc tatttatcgt aaattatatg aggtgtaact atgtctttac ttacatttga
    20881 acgcaatctc ttactaaaaa acaaagtaaa ctttttattt cctctattgt tggtcgtgct
    20941 ctttgcattc ccactttttt ttgatcataa acttgcttat acagaattcg atgaactgaa
    21001 tcataactat gaagagatgc aacgtttgat tgaaacgttg aaagaagatg agaatgagaa
    21061 agaatttgtg gaaagtttag agaaaagcaa caaactgatc gaagcaatct tacatgcaaa
    21121 aaacacaggg aatgtccagc aaatggtcga agcaacttat cattatgaaa aagatatact
    21181 ggatcgactg atttctggtc aaagacaagg aattccaatc attgaacaac aaaaacgtgt
    21241 cgagttgctc cgttatatga aagaacatca gatccaaaga tattctattt ttgacttgcc
    21301 agcgcatctc tctttagcaa attactacga aaatattttt tcaggaatga tttctagttt
    21361 tttgatttta tgtataaccg ccttgtttct ttcttccatc atctcttatg aaaaaagaaa
    21421 gcaggtaatc tctctagtca atctattacc ggatagtatg gtaaagaaac atagcatccg
    21481 ttttaccatt tattatggtg cagctatgct aagtctcgtg atgccatttt tgatcgtctc
    21541 tatactagta ataatcaaaa atggtttggg agacttccgt tatcctgtag gaacgatcat
    21601 tggtcaggag atacgaatcc tgccgatgta tgagtatctt ttccagagct ttctgttcct
    21661 gttgttgtgg gtgctctttt taagtacaat cagttttctt ctatctgctt tatttgaaca
    21721 ttctctagtc aatcttttag gtacacttct ttgtcttttc ttagcagagt atcgcttgtt
    21781 ttcttcgatt ggatggatag aatcaatcag tcattacttg ccaacttctt atgttgattt
    21841 tcaaaatgtg atcataggag gagatatttt ctcgccacta gcttcagaac aggttacttt
    21901 catgaatggt atcttgacac ttggtatatg gagtatcgtt ttattattca taggtatggg
    21961 cactatctat ataaaaaaga gctattaaaa cacaaaaata ataggggcgg tgacaaaaac
    22021 cttgtcaccg tcccctattt caaataaacg gttttctaga ataaatagtt ttcaaaagct
    22081 tgcatccgca gtaataagaa cctcccaaca aaaatagctc ctcttatttt tatcttggtt
    22141 gaagttatta cttgatgcaa ggcagctttt tttactgtga acattggtta tatatgtcca
    22201 tgttcaaaaa tcatttggac ttcttcaatg gtttccgcat cactgagcgc cattacttgg
    22261 tcgatgtctg aacagaaaaa ggctaaatct tggagcaccg ctaactgatg ttcttttttt
    22321 aaagccacta caaataagag tgtcgcgatt tttggttcct cttttgttcc aaagtccaca
    22381 ccatcaggca cttggactaa atagattcct tcttctacta cttcttcatc tttgtcagtc
    22441 ccatgtggca aggctacgaa attcccgata tagacactcg ctttttcttg tctttcgatc
    22501 attgattgga tatatgccgg tttgattccc ccttgttggg tcatccactg gccgatctcg
    22561 gtcaacgctt ctttccaatt ttgataggat tgatcgagca atattttttc tgctggtagc
    22621 tgtatcatct tttcaccttc atttctttat ttcttcaatc aggattttcg ctaacaactg
    22681 ataaacgatt tctttatttc ctgagcggaa gatctcggtg ttcaagtcat tcatgatgat
    22741 tgctccactg atctttccta aaagaatccc gtctttttct tggattggcg aaggcgccag
    22801 cataactaac atacgggaca aatcgactgg ctgtctatcc atcccttcaa tggtcaacgg
    22861 atgtttcaaa ttgaaaatac aaaagatcgg tttagaaatc aatgagctgg atgtatggaa
    22921 taaccccata tttgtatgag gaataccgat aggtgcttgt tcatatcgct taagcagccg
    22981 gcgatgaacc acctcttcat ccgttactag tccttcagga aaataaggaa ataagctgcg
    23041 aatcgtttcg gaaatcgtct caggattatc cagttcttgg ataaaaaaac gaccgagtaa
    23101 ttgattgatt tgatccatga aatgcattgt ttcctcataa gtatcttcta tcatccactc
    23161 gttattgctg ttatacgtaa ttttttgcct ttttttcttc tcaatatgcg aaaaagcttc
    23221 ttttagctgc ttgacttcat cttctagcag tagcggggag actaaaatat attttccacc
    23281 gtatcctggt aaaatagatg tagaaataac aacatcatat tcttcttgta aatcgatttt
    23341 ttttaaatca gcaatccgat agaaatgaat ttgttggatg aagggaaaat accttttcaa
    23401 tcgtagttcc aacatgctag tagaagctaa tccacttggc gaaaatcctg caatactgat
    23461 attgctttct ttcggacttc gttccaatga attcgcaaaa tgaaggacca tataagcgat
    23521 ctcttcttcg gataattttt tttcttgaaa aatccgctct actgctttac ggattgcatt
    23581 cgcaacttcc tcgtattgaa ccatgatttt ttctaaaatc ggattggtca gtgtctcttc
    23641 ttgtaaaacg gctcttgaaa acacaccaga taaatgagtc aacagcattt tgtacaaagt
    23701 ttcatcttca taaaaatgga cattcgtctc ttcacttact tcttgaatca gttgttttac
    23761 taaataagca agctgtgtat cgaacgtctc ttcaaagaaa ttctgatcaa atgagttcgt
    23821 gaagtcattt aataatcctg caaaaaagac gatctcattt actggataaa gttgttttgt
    23881 ctcagaagcg atccgtgaaa acaatcgttt agctgtctgt agtaattctt tgtttgccag
    23941 actattatat gtctcatttg tcagtaaatg atttttcgtc acacgctcca tcgtcaatgt
    24001 cagcatcaaa accaaatgtt caagctttct atcacttaga ttggtctttt tttggatcaa
    24061 ttcttccccg attactttgc gtgcaagaga tagatgttgc ggatcaacga atcgaaaaaa
    24121 ataattcagt gatgcatcga aagaaatact atgaaaaagt tcatattcat tgatttctaa
    24181 ttctaataca ttcgctgtca gaagccggcg gtatttttcc ggtccttcga tctcgtatcc
    24241 ctggttccga atgatttcca aaggcagttg gcgaatactt tcttccaact gtttgatatc
    24301 tgcataaaaa gtggtattac tgatgcgata tgattccatg aaatgagcaa gactcaacgg
    24361 ctctgtcgtc agtaacaact cggctaaaat cgcgtgttgt cgtttagcag tggataagtc
    24421 attacttttc ccatctgtat tttcttttaa ttgctctaat acttcttctg atgcctcaat
    24481 gacaaatgag cctctagctg cttttttcaa agaagcattt accgatttca atgagacttc
    24541 aagattatct aattcgcggt aaatagtccg tttactgacc ttcaataaat tcatcatttt
    24601 ttggatagat acaggtgacg ggctactgag aaattctgct aataattgtt tttctctggg
    24661 cgataaatac atcttatatt tctcccttca cgagtttttc gtattcattc gtagctaccc
    24721 aatgttcaac tagaactagc cgagggatct tttcttctag ctgttcagtt acagctagtt
    24781 ctctttgtgc gatataaatc gtttcttctt ggattttgat tttttctatt gattcttttg
    24841 agataacaaa ctgttttttc gctttttttg ctaattgact taaaatcgtc atgcccatag
    24901 tttgtgagcc gcgtctgttg tttttataca aaaatacaat attagcaggc gacgtttttt
    24961 gttctgtgat atctgctatc tctttttttt cttcccgctt tggaaattgg atcgcttctt
    25021 ttattcgtga cagcaagata tctgtttcca aaaagtgatc cagtacatag atttgacttt
    25081 gaggtacttt ttctcttgca agacccgcta gttcatgttg cacgataacc agctgattta
    25141 cttggttatt caattcatag atagagctgt atccgacaga aagatcgatt ttttctgctg
    25201 ctaatttctt tttcagcagg gcggctccca tagcacttga tcccatacct gagtcgcaag
    25261 caaaaatgac ggaatgaatg atagatggtt ccaagatttc tcccctttct ttgaccgttt
    25321 tgttctcttt ttctttattt ggtaatttca atagtatcgc tgcacaaatg aaggtcacaa
    25381 atgtactgat tgctatccct atacttaccc cagctagttg tggataaggc gcattggcaa
    25441 aaatcacgag tagtgaccca ggcgatacag gtgcagctaa tcccgtatct aaccattgaa
    25501 atatcaacgt gccgcttgcc ccaccagcca ttacagctaa aaatagatac ggtcgcagca
    25561 agacaaatgg aaaatagatt tcgtgaatcc cgccaatcgc atggatcatc aatgctccac
    25621 tcgcatttac tttttcctgt ttttctgcaa aacaaaggat agaaagtaag atccctaaac
    25681 caggtccagg atttgcctcc atcaaaaata aggaagagta cccgactgtt tgtgagaatt
    25741 cgattcctaa aggtgtcaat acaccatgat tgattgcatt attgaaaaat agtactttta
    25801 gaggttctaa gatcagactg acaagtggta acagattcat tgtgactagc caagacacaa
    25861 cggaggcgag caacaagtga acactattta aaactggttc aataaaaacg attccaatta
    25921 tacaaataac gctaccagtc agtcctacta gtaggttgct gacaagcatt tcatatccag
    25981 ccttgaattt cacatggaat ctagaaacaa attgttcata tacgatgccg gcaagcggcc
    26041 cgataatcat cgctcctacg atttgtggag cattggaacg agcgatcaac cctaaagaag
    26101 caattgcacc ggcgactgcc cctttttgtc cccctaccga tttcccgcct gaataactga
    26161 tcaagatggg taaaagcagc tggatcatcc aatggcatac ctcttctaat gtggtgtgca
    26221 aatcgccaga cacacctttt gttgctaatg acaagattcc ccaagcgata aacagactta
    26281 gatttggcat gatcatttca tttaagtgat gtccagcttt tctcagttgt ttcatagaca
    26341 cgcgactgct gttgtttgct ttccatttgt attccatctt tttcaccaga cttctattta
    26401 ttgatggacg gattttatgt agaaaaagca gcaaacgaaa aaccgtctgc tgctttcagc
    26461 tatctgggaa ctatttgcct ggttcagacg caatgattgc tagttcacct tggaccgccc
    26521 actcagaaac gccatttgga ataatacagc ttgttccttt ttctaatgga tatgtttttc
    26581 cgtcaacgac aaggtctcct gctccttcga ttaccgtcat caatgtataa ggggcttgtt
    26641 ttttgaaaga tgtgattcct ttaatatccc actcatatac attgaaaaac tcagtttcta
    26701 aataagtgac gatcgatgaa ttcccttttc ggacttcttt gatttgtaat tctggtgttt
    26761 tcgcagggac agttgttaca tcgatcgatt gttggatatg cagctctctt gtttgtcctt
    26821 gcgcatcttt tctgtcgtag tcatacacgc ggtaagtcgt gtcagaactt tgctgtgttt
    26881 ctaaaatcat gattccttta ccaatagcat ggatcgttcc gcttggtaca taaaagaaat
    26941 cacctttttt aactggtact ttcttcaaaa ggtcatccca gcgcccatct ttgatcatct
    27001 cagctagttc ttctcttgtc tttgcatgat gaccatagat gatttctgca ccaggttcag
    27061 catcgatgat ataccagcat tcagtttttc ctaattcacc ttcatgtttc atcccatagg
    27121 catcatctgg atgtacttgt acagatagat catcttctgc gtctaagatc ttgatcaata
    27181 atggaaacac tggttctgaa ggatggccaa acaattcctt gtgttctgac catagttcat
    27241 ctaatctttt ccctttgaat tcgccatttt ctactacact gacaccatga ggatgcgcac
    27301 tgatcgccca atcttctccg attttatcgt tagggatatc aaaaccaaag actgaacgta
    27361 aacgacttcc tccccaaatt ttttcttgaa aaactggttt taaaaacata ggttcctgca
    27421 taattcaaac tcctctcatt tttcatagct attatagcat tatttcggta ataccgcttt
    27481 ctctttcaaa aagttttcaa caatttcatc tcgacatatg agatactccg cacgatgatc
    27541 gacaggatgt acgcgatttg acaaaaatat caacgcttct tgtgttttta tatcaatcaa
    27601 gaaaaacgta cctgtataac cagtatgaaa aagaatcgca tgaccgtctt ctacatcctt
    27661 ttttaagtcc catcctaaag aacgatagcc attttttttc ggtgtttgat cttgcagtaa
    27721 agaatcaatc gtctcttctt ctaaaaaacg accattacgg atttttccct tgttcaaata
    27781 catttggaca aaagtaatgc tgtctttcaa atcaataaag agacccgcgt tacctgcatg
    27841 ttcagccaaa actcttgctt tcggatcatg agtttgtcca cgcaaaacct cacccgaagg
    27901 cagttgttct gtgggaacga tttttttgga aggaaaagga agaaataagc tgttcatcat
    27961 tcctaatggt tccaatactt gttcttgaaa gatttccact gcagtttctt ggtaaatttc
    28021 ctctagcatc aacccaagaa gaatcgtacc ggcatctgta tattttactt cttttcctag
    28081 ttgctcacct ggttctagct tcaaataagc ggcgatcaat tcttcctgag atagcttatc
    28141 tctgtttggt atccatgttt tgatatcaga agtatgtgtc agtaaatgac gcaaagtgat
    28201 tgtctgattt tcaaattcag gaagatattt atgaagcggt tgatcgagtt ctatctttcc
    28261 atcttctaat agacgtaaaa ctaatgaagt ggttgcaacg actttcgtca gtgaggctgc
    28321 atcaaacaac agatcttctg tcatcagctg cttttcagga acgacttggg caaagccggc
    28381 tgtaaatgtt tctacttgat tttcttttat gaaggcgact gctacacctg gataaaatcc
    28441 ttcttccatc tttttcaaaa taagctgttt cgtgtgtgga tacatctttt tctcctgctt
    28501 ttctatcaga ttcttacagt ccaagcttaa agcttaatct atgaggaatc aacctttttt
    28561 acacaaaaaa actgcgcgaa aaaccgcgca gtttcccccg gtatactatt ttctctttct
    28621 atctctagaa taagcgatag tatgaccgtt cataaaatga cgagacatgc gacaatcgtt
    28681 aagaaagaaa aagttaagcg tcgcatcgaa tgtcagtttt attcttttct tttggctcac
    28741 tgacgaacca ccattcgatt cctatagcat catagattgt gagtactgtg tgctttttgt
    28801 ctaagaagaa atcaactgat agttgctgca agtgagcaat cagtccttcc atgctttcag
    28861 tatcgatcat gaatttcaga aaatcaagtc caatcacttc atctgtaggc aaatcaattg
    28921 cgcctccgct agcttctgtc aaaccaacat ggaaatctcc ttcattcaat acatggatgc
    28981 cctcttcttc attatgtacc ttcaaaccaa gtacatgatt aagaaattgg cgttcttttg
    29041 ttaagttagt aacatttaaa tggatttttt caaaataaga acctgttgat agtttactga
    29101 atttttcttc agattttctt aataatgctt cttgatccat tgtttctgga atttctcttt
    29161 ggtcgttctt attctcttca tggtaaattt ccaattcatt ctcttctggg tctacgagca
    29221 aaatccccat acgtccatga tcttctaaag cttctttgat tggataattc attttacgga
    29281 tacgacaaag gatatctgcc atttcttctg tgctagggat aatcaatgaa aaacggttca
    29341 gctttttgat cacccccatg tgatcattcg ctctaggact ttcttccagc agcaacattt
    29401 cacttccagc ttcttctgtt cctaaaatgg ctaattcgtt ttcttctctt ttcaaatcaa
    29461 agccaacaac gttcttgtaa aaatcaatca ttttatctcg gtctttcacc cgaatggcta
    29521 cactttttaa ataggtggca gcccccaatt gaaaatcttt catatttctt gctcccctct
    29581 acgtcaagct ttattttaac gatggattct tcttctttca agcaataact ttgattttgt
    29641 taaaaaaaat gtgagaaaat tcgcattttg cttgtttttt gtaaattaat gagtaaaaac
    29701 tcttataaaa agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca
    29761 aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
    29821 acaccgttta aggaagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt
    29881 tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga aactggcttt
    29941 tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctccgagtca caaggagcaa
    30001 tttcaaaaaa cagcttctca ggtttgttga ctattgtgac ttgtgaccga ggagttggca
    30061 ggagaacacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag tattagagat
    30121 acaaaacgga aaaatagctt ctcatatttt gccgtttttt gagctaatac cgaaggacag
    30181 ctgattgaac accgtttatt cggaaaaagg gactgtgaca cgacttttgt cacagcccct
    30241 aaaaaaaata ataaatttgt actattctat gaagcttctt cttcagaaaa ctgactgtta
    30301 taaagatctg catagaatcc gtctttttcc atcaatgtat cgtggtttcc tgtttctacg
    30361 atcgaaccat gattcatcac aataatgttg tctgcatcgc gtattgtaga caagcggtga
    30421 gcaacaacga aacttgtccg gttttccaat aggcggttca tcgctttttg aatcagtatt
    30481 tctgttcgtg tatctacact agatgttgct tcatcaagga tcaggacatc tggatttgct
    30541 aagaatgccc gcgcaattgt gattagctga cgctgccctt gcgaaatatt gcttgcatcc
    30601 tcgtttaata cagtgttata tccttctggc aatttacgaa caaaatcgtc tacgtgagca
    30661 gctttagcag cctcaatgat ttgttcgtct gttgcatcgt cgttcccata tttgatattg
    30721 tcatagatac ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa ctgtgcgcgc
    30781 aattcatcac gtgaaagatc acgggtatct acaccatcgt aacgaatact acctccagaa
    30841 acatcgtaga accgttcaag aaggttgatc agtgtcgtct tccctgcacc agtcggacct
    30901 acaatagcga ccatctcacc aggtttgaca tccaagttaa aatcagtcat tagcaagtta
    30961 tctggtgtgt agccgaattg aacattttca aaacgaactt tgtatggtgt atctttttct
    31021 tctggaaggc cagatttttc attggacatc tcttcttcat ccaacacttc aaatacccgt
    31081 tcggctgaag caactgttga ttggatagtg ttcatcaaac ttgcaagttg agtgattggt
    31141 tgagagaact gtgttgtata ttgcaagaac gcttgaacat cccctaatgt catgttccca
    31201 tttgcaactt ttactccgcc tagtacagcg acgaacacat agcctagatt tttaacgaaa
    31261 ttcatcaacg gcatgatgat tgccgaaata aattgagctt tccaaccagc ggcatacaga
    31321 ttctgatttt ctttttcaaa tacttcctgg tctttttcag tatgattgaa tgttttaaca
    31381 atggtatgac caccatatgt ttcttctact tggttattca ataaacctaa acttttttgc
    31441 tgatctgcga aaaacacttg tgatttcggc gctacgatca tcacgatgat caagcttaaa
    31501 gggatcatca ataacgcgac taacgtcaat tgccagctga ttgaaagcat catcacgatg
    31561 accccaatca atgtgactgc actagttaca aattgagtca aattttgctg cagcgtgctt
    31621 gcgatgttat ccatatcatt gattgctcga gacatgatat ccccatttgt atgtgtatca
    31681 taataggaaa tcggtacacg attcattttg gcttccaaat catgacgtaa ttcataaact
    31741 gttcgttggg aaacacgtgt cataataaat tgctgtaaaa agttgaatac agcagaaatc
    31801 aaatacatca cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc tatcggaaat
    31861 tttgttactt gttggccttg ttgcatctgt tgagcgccag ccattacacc tttgaatatt
    31921 tctgtggttg ctttccccaa gacctttggt gtttggattt ggaagatcac ggctgctatt
    31981 gctagtacaa atacagcgat gatcgcaacc atacgtttag acatatagcc aaacaatcgg
    32041 cgtaccgttc cccagaaatt tttaggcttt tcacctttta caccgagatt tctgccaggt
    32101 ccaccatgtc ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt tttactcatg
    32161 ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg tttgttgcta
    32221 atagttcttc atgtgttcct ttgccaacca gttttccttc atctagcaca aggatcatat
    32281 ctgcatccat tactgtactt actcgttgag caactaaaac ggtgatactt tctttcatat
    32341 tcgttttcaa tgcttggcga agattggcat ccgttttgaa atccaacgct gagaaggaat
    32401 catcaaacac atagatatct gctgttttga ctaatgcccg tgcaattgcc agacgctgtc
    32461 tttgtccacc agagaagttt cctcctcctt gttccacatg gctatctaag ccatcttcca
    32521 tctctgaaac aaaatcttta gcttgtgcga tttctaatgc tttccaaatc atttcatctg
    32581 ttgcatccgg cttaccatac tgcatattgt ctcggatcgt tccagagaac agaactgctt
    32641 tttgaggaac aaagcccatt aattctcgca aattatattg agtcatgtca ctaatgtccg
    32701 ttccatttat ttggatcgaa ccagattcga tgtcatataa tcgagggatc aaattgacta
    32761 aggtcgtttt acctgaacca gtccccccta tgattgcgac gatctcgccc gacttggctt
    32821 ggaaatcaat atcttccaat gcaagttttt ctgctccatg atatcgataa ttgacatgat
    32881 tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt tttaggattt tcagggtcat
    32941 ggataccgat tttttcatcc aacacttcat taatacggtc agctgaagct tgtgcacgag
    33001 gcaccatgat aaaaatagcg gaaagcatca taaagctgat caagatctgc atcgcataag
    33061 tcataaatgc aatcagatta ccgacttcca aagtcatatc tgctatgtag tgtccaccaa
    33121 accaagtgat tgctacgttt gtaccgctca tgatcaatgt catcattggc atcatcaatg
    33181 cgacgatcgt attaactttg attgccgtat ttgtataatc tttattcgct aaatcaaaac
    33241 gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct gtcaatcctt
    33301 ctcgaaatac caggtttaaa cggtcagttt tcttttgcat acttttgaat aatggaacag
    33361 caaagtacat gattccgccg atcaaaataa tcattactgg gatgacaact aagaagattt
    33421 ttgtcaactg atgatctttt tgataagcaa gtatgctcgc gccaatcaac gtgattggtg
    33481 cattgatcat catacgtaaa aacatttgtg taaccatttg gatttgagtc acatcattgg
    33541 tcgttcttgt gatcaaagag gccgttccat atttatcaaa cgcatctttt gtcagactct
    33601 ctgatttttt atagacatcc gtacgcaatt gcttacccag tttctgtgat tcacgagtag
    33661 caaaaaatgt atttgcgata gcagctaaga tactgatcaa agaaaagccg atcatcacaa
    33721 aacctgtatg ccaaatataa tcgacatctc cttgcgctac ccctttatca ataatattgg
    33781 aggtaagtgt cggcaaatat aaatctgcca gtacttggat caccatgaaa actgcagctg
    33841 caattgctga agtcaaggag atccgtttta cgatttttag catctagttc ccttctttct
    33901 atttatatac tccgtttttc agccaagaaa gtttcaaatt gaattcactg attgcttgct
    33961 ctactgagta atcttcagat gttgaaaata agcgatacga atgcgcaatt gttgtaaata
    34021 cagtattcat cactaattgg atcagtaatt ccaattcatg atcatttttt actttcagct
    34081 tcgtccgatc gatcaattca agcaactctt ttgcatcttg tttgtgttct ttatgtggat
    34141 cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt tcttgaggca cggtaatcca
    34201 tgttcataaa caagttacga taaaaagaag cattctcgcc ttccaaaatc tcataaatca
    34261 ttttagaaaa ataggattcg aatcctttga atagatcacc atcggattct ttcattagtt
    34321 gtaacatatc tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa taaagatcct
    34381 ctttatcttc aaaatactga tagaaactgc ctctagggat atccgctaat ttgacgatat
    34441 tagcaatcga agcttctttt aagggtactc tagaaaactc gattctcgca gcctcgatta
    34501 gtcttttttg tttttcttta ggcaaatgga aaaatgtctg tgtcggcata tggattctcc
    34561 tttctttcaa gatgacacat tgtcatatga cgctatgtca ttataacacc tcctacctgt
    34621 tttgcaagac atttgcatag tttttttcat tatcatccga tacattaaaa aagtgataag
    34681 aggaatcctt ccatttgaat taatgagagt tgcccattaa aatagccaca tactaaacaa
    34741 aggatgagat aaatggccca atcaattagt gtccaagact tcaataaatt gcctctcgat
    34801 gctgacagca tcgttttaga tacaagaaat accttagatt acacaaaaaa ccacttaagc
    34861 cactcgcttt ctatccccgc tgctatcttg ccaagacaat taaaaaatct agatccaaag
    34921 aaactttatt atattctaag ctatacaggc aggcgatcag aagcattagc cgatcaactt
    34981 actgctaatg gttttcgtgc tgtttatatc attggtggga tggaagctct tcagtcgagt
    35041 gttgcttgaa tattgcttgt tagattcaaa agaggctgtg acaaaaaatc gtgtcacagc
    35101 ctcttttata ccctgtcttt ttttagttat ttattcttca accttatcaa tatgacttga
    35161 tagccttttc tttttcgagt aaactgattt tgttagtcat agttataaca atcatagcaa
    35221 taaaaggaga aggtaagcat atggcatctg ttttagtagt caaaggtcat cccctaactg
    35281 cagaagaatc gcgcacagtt aaggcattga cgtctttttt aacaagttat aaagaaaatc
    35341 atccagatga tgaagtgact gttttggaac tttatcgcga tgatattccg gaaatcgatg
    35401 aagaattatt atcaggttgg gaagcattac gtgctggtgc agaattcact tccttatcag
    35461 aaagtcaaca acaaaaaatt gctcgtttca atgaattgac tgatcagttc cttgctgctg
    35521 ataaaatcgt tatcgcaaac gccttatgga atctaaatat cccaacaaaa ttaaaagctt
    35581 ggttcgatac cgtcaatgta gctggaaaaa cgttccgcta tacagaaaac gggccagaag
    35641 gattagtgac aggtaaaaaa gcactgcaca tccaatctaa tggcggtgta tacaacggac
    35701 aagactttgc ctctcaatat gtaaaaggta ttttaaactt tgtaggcatc gatcaagtag
    35761 atcagctatt catcgaagga atcgattatg accctgaccg tgcagatgag ttgatgcaaa
    35821 atgcattaga taaagctgct gctttaggta aatcatttta aaaattgaga taaatgcata
    35881 caaaagagac agaggaaaaa atcttcaaag cagcatttgt gccttggata ttttcccatc
    35941 tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa gagaagagat
    36001 tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat aaacaggaat
    36061 ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa ctaataaaat
    36121 cgttactgga tggattgcta actgattccc tagtactaat ggagagatca agcgtccttc
    36181 tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg gacctgctac
    36241 aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag ggatcaggtt
    36301 taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga ccgcaaaacc
    36361 aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta tataagaaga
    36421 aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc gtgtcggtaa
    36481 aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa ataagataaa
    36541 cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac ttccaagact
    36601 ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca tgaacttatc
    36661 acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca aaatctcggt
    36721 taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc tcactgtttg
    36781 ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa caaccaaaac
    36841 aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt aatcaactac
    36901 cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta cgattgctaa
    36961 aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga cgatcagcaa
    37021 tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc agcgccaaaa
    37081 ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg ttagttataa
    37141 aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata gattccattt
    37201 gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc tactgtcttt
    37261 aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc atcaaaagca
    37321 attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg aatcaccgaa
    37381 tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg ttcttccttg
    37441 tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact ctctccttta
    37501 attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa taattatgtg
    37561 atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca tatgatcgaa
    37621 aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac gatcgacctt
    37681 gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa tagccctacc
    37741 tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga tggattaggt
    37801 ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga gtctggcgct
    37861 cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc gaactatcat
    37921 aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc agcagattca
    37981 atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca tgtccactat
    38041 acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga tcgtgtgtat
    38101 acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac tgcagagcca
    38161 ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta tttatttggt
    38221 gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc ttttgaagaa
    38281 ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg ggcagctatc
    38341 cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa ctcgattgct
    38401 gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat ctcaacagaa
    38461 ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt gtgtgccaat
    38521 cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt actggaatta
    38581 ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg ttagtaagaa
    38641 aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga actaaatatt
    38701 cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata tcagaaactc
    38761 aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa ctaatcagct
    38821 ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct gatgttcttt
    38881 tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta tttactcgat
    38941 tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa aggattttga
    39001 tcactctatc ataaaggtaa tattacattt atattagata actctagttt ttattgttat
    39061 caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg cagctgtcaa
    39121 ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt catctacccc
    39181 tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg aaagataagc
    39241 tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag tactgacttt
    39301 cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga tctcttcaag
    39361 agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga tcacttcata
    39421 tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat cgatggactg
    39481 cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat tgttgttatt
    39541 atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt aatggtttct
    39601 atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta tattgtttcc
    39661 ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt tgcgagcgat
    39721 tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt tcaaacgccg
    39781 gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc tgattcgtct
    39841 gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga gtcgtttgct
    39901 tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat gtttgtttgt
    39961 cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc caaaaaacaa
    40021 ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt gccgtgctgc
    40081 tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca tctgaagttt
    40141 ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc tgcatcgtga
    40201 ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact tctttgtaag
    40261 ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc atcgtacgct
    40321 cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta tacatcgaag
    40381 tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct gaaacgatag
    40441 aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt tgcgcattaa
    40501 ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt ccaccgccat
    40561 agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa taatctgcca
    40621 atggctcacc agatacttct acgccgttta atcgactatg atctgagaaa ctttcactca
    40681 tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca tcggctagct
    40741 tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca ggttcatcca
    40801 ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta tagtagttca
    40861 ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc aacaaattat
    40921 cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc agtgtcgtta
    40981 atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac cagcaaaaca
    41041 atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg ccggtcatat
    41101 ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc ggttcttgcc
    41161 gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg acatacgtaa
    41221 gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata atcagcaaga
    41281 cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc aacgcaaggt
    41341 ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca acaagaaaaa
    41401 agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc gaaagtctgc
    41461 ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat gttgcttctc
    41521 ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa tgaaatccaa
    41581 atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt cgtactccag
    41641 aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta ctgtctatgt
    41701 caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg atcgctagct
    41761 cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt tgataaaata
    41821 tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg accgcttctc
    41881 taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt ccgctgtctg
    41941 ccaaagcttc ctgagtcgat ccccctagat gaggcaacat aagtatacgg tcattttcta
    42001 agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt tccaatgctt
    42061 gcataacagc atatttatca acaatttcac ttcttcctac attgataagt acagcatctt
    42121 ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct gtcaacggca
    42181 gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct tgccgaacat
    42241 aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag ctatagcaac
    42301 ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt ttgccttgca
    42361 attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata ttaggacctg
    42421 ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag agcaaacagc
    42481 acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg acgatcgtcc
    42541 cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct cttgaaattg
    42601 ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata actattcctt
    42661 cggctggcaa atcatctatt attttgaaat attccccttg taacttttct atctcttgtt
    42721 ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac taacaagtat
    42781 ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag caaaaagagc
    42841 agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac tgccataagc
    42901 cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg ttccgttagc
    42961 tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg tcaatctctt
    43021 cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata accactgttt
    43081 ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt agcgagacaa
    43141 cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg aaattcggtt
    43201 tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt ggttcaaagg
    43261 cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc gatacgacta
    43321 ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta tctcccattg
    43381 ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct acttccatca
    43441 tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa tcttccatga
    43501 atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct gcagttttcc
    43561 agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca gctagtccag
    43621 gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta tagcctcctt
    43681 cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca tgttcctccc
    43741 atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac tcttcaaata
    43801 attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct ccgataccaa
    43861 aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct tctgtgacac
    43921 ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt tcattatagg
    43981 ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt ttccagtagc
    44041 caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat tcgcggtcac
    44101 catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa ggaatgcttt
    44161 cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc ccgcggatcc
    44221 acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact tcttctcgat
    44281 aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat tcttgaaggc
    44341 ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct tgtgcatgtt
    44401 tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca acaaaccaaa
    44461 attctttttc tccaatattc aacataatta gccactcctt ttatttgttt tgtccgtaat
    44521 acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga ggcagctctt
    44581 ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca tctagtacga
    44641 ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg tgtccgtgta
    44701 aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt tctatgataa
    44761 cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa aatcgagcac
    44821 aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga acatccaatc
    44881 ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct ccgatttgag
    44941 gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg agtttacctt
    45001 caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc tcttcataac
    45061 tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg ctcacatttc
    45121 cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg tatacttctt
    45181 ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg aatcgctgtt
    45241 ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc tgctatttct
    45301 tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc tgccaagaag
    45361 ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc gattccccac
    45421 gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc agatgctaga
    45481 atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa acgatcaatt
    45541 tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa agcactggct
    45601 aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg tacaaataat
    45661 ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata agaaagtaat
    45721 cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa taatttatct
    45781 ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac ccaagcattg
    45841 atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga aggtgtagtg
    45901 accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat catagcgaag
    45961 gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt agcgaccata
    46021 ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa atttcccgta
    46081 gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc tgctactaga
    46141 actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat taactgacca
    46201 aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg aaacatgcct
    46261 gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac ataacctgca
    46321 agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat agattgatac
    46381 aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt taatattcgt
    46441 tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa ctgttccttg
    46501 ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc gattgtcgta
    46561 agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact gacgcgcagt
    46621 cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt gttttcccac
    46681 tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc tttgatccta
    46741 gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa catactttgc
    46801 gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg atcaaaccgt
    46861 ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt caagcagtta
    46921 tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata aaatgaaatt
    46981 gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat acttatgaaa
    47041 aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg cggcattcta
    47101 tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca tgattggggt
    47161 ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt aggtgcgatc
    47221 cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa aatgattctt
    47281 ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc tcacaatggt
    47341 tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc tgcatctgcc
    47401 ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag attgtcaggg
    47461 atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga ttatttgcta
    47521 aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc tgtccccgcg
    47581 ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct tatgcaatct
    47641 ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa agaagctgaa
    47701 caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg aacttcttgg
    47761 catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg tgtagcagta
    47821 ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat cgtagaaaaa
    47881 gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg aatcatttta
    47941 gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg aactttattg
    48001 aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt aggaactgta
    48061 ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta tgtcgcattt
    48121 tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt cccactagct
    48181 gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc tttcctagtt
    48241 ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt cggcattttt
    48301 gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga aactcgtggg
    48361 aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa acgtatttct
    48421 atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaac
    48481 cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgatgaata
    48541 ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg tgaaagaaac
    48601 gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa ccgtaggagc
    48661 aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt ttcccgttca
    48721 actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat cgttgtgacg
    48781 atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt tgtcaatttt
    48841 ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag gtcatctgga
    48901 atatgatagc cattttctgt caacttatct agcattaaac tagctatttg atcgttatag
    48961 caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt ttcagcgatt
    49021 gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg cacattatac
    49081 tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc gtcgattttt
    49141 gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc cataaaaccg
    49201 actttcacat catctaaaca aatataagag gcatttaact cttcataagc agcattgatc
    49261 atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt tgggttatac
    49321 tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat cattttcttc
    49381 aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa tgaataccca
    49441 gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata atcagagaga
    49501 tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc tttcttatag
    49561 ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc atttaccaac
    49621 acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg cagcttgaat
    49681 tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg ttttatctct
    49741 tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta cgtacatata
    49801 tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg ctcataaggc
    49861 atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa agcattgcgc
    49921 gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct ctggtcgagc
    49981 atgatagatc aaaatatcaa agcacccgtc ttctgaaacc gtaaacgagt tgtgaccagg
    50041 accaaattgc ttattttttt cagaagaaac gaatactggc tccgataatt tatgccatga
    50101 ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg catagttttc
    50161 atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa ggactgctgg
    50221 accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag aaaggaggat
    50281 ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat tagaattccc
    50341 agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag aagagaaagt
    50401 tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt ctatccaatc
    50461 tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat gatgagattc
    50521 atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc cattgatata
    50581 atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag cctgccatac
    50641 aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta attcaatcac
    50701 ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt tatataccca
    50761 aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca taacaaattt
    50821 cctcacattt tctttattct aattacattc caagagtatt tttctatccc tacatttaat
    50881 tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc tggttcttca
    50941 cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc ttgatctagc
    51001 aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt gatcaagaaa
    51061 atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac attttttatc
    51121 ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc tttagcatat
    51181 ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc tttagcatct
    51241 gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt tacgcgatct
    51301 gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc catagtaaag
    51361 taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc cggttgggca
    51421 tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa atatcctttt
    51481 acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata agcttttgca
    51541 aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc ttcttcatca
    51601 tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc ttcttctaat
    51661 gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc aactaagatt
    51721 gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc taacctacca
    51781 tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt ccctaaacac
    51841 cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc actccagaag
    51901 gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat tccttttgtt
    51961 cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt aaaaaattcg
    52021 tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt ttttggtcgt
    52081 tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt tcctccagga
    52141 tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg tctaaacccg
    52201 tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac acttcccata
    52261 tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa atcctctctg
    52321 atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc tatagtcaag
    52381 ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg agggaaaaga
    52441 aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct caaaaaatca
    52501 aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc tttgttttca
    52561 gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac tacatatcca
    52621 ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa ataaataatg
    52681 atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc ttgaaagaaa
    52741 aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata aaaaacagca
    52801 catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag aatacctaat
    52861 aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac tattttcatc
    52921 ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa
    52981 tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca ggtccataaa
    53041 aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc atttttgaac
    53101 tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc gtgaaaatcc
    53161 ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa atattaaact
    53221 cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg
    53281 aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa cctgcataag
    53341 tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag acttggaatg
    53401 ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg aaacgcatac
    53461 gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact gtccctccag
    53521 caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa accttaatat
    53581 agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata atgtcttttt
    53641 cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc aacaatataa
    53701 ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga ctattcacag
    53761 taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat ttgtttttct
    53821 ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat aaacaaaacc
    53881 gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg actgtaaata
    53941 tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac atatacttgg
    54001 tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa ggagggaacg
    54061 atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc catttgagca
    54121 gattcataca aagagggaga gattccctga atagcagtca agagaatcgt catagtaaca
    54181 ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt tggatcgcga
    54241 aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc ccctacattc
    54301 ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat aactggaatg
    54361 taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc tactgctgct
    54421 aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc
    54481 aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa acttacaaaa
    54541 tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc
    54601 ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa atacgcagca
    54661 cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc atctttcatt
    54721 ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga acaatcgctg
    54781 tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat atctgtatct
    54841 tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg attgatggca
    54901 tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt ttcaatcagt
    54961 ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa atatggctct
    55021 gcatagtcca tttgtttcga taattcagat acaataggat cattttttac ttcttcatct
    55081 tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt tttaccaatt
    55141 tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt attatctgta
    55201 cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc tgaatcactc
    55261 ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc gttatttaac
    55321 catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc catttcggcg
    55381 ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat cgcttttaac
    55441 gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt cactaattta
    55501 ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt gaagacaaaa
    55561 cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa ttcttcccaa
    55621 gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt attccaatac
    55681 atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac ttcacctaat
    55741 tcaatagccg tttctttaaa atcagattta tctactccat catattccca gaaatcgtct
    55801 aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt taatgcaata
    55861 aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa attggcccaa
    55921 gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa ttcattaacg
    55981 atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt caaagtcact
    56041 tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa aatacctgtt
    56101 gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg ttcctccttc
    56161 atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac aattgatttt
    56221 cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac ttttaggaag
    56281 attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc cctctaatga
    56341 taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa ctgcaaatat
    56401 gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt cttcaaaatc
    56461 ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt gtccatacac
    56521 agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt
    56581 cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta ctctatctgc
    56641 atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa aattatagtt
    56701 atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt gttccgctga
    56761 atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac tatgtttctt
    56821 cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga
    56881 acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat gcagagacaa
    56941 ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac caaatgtagg
    57001 catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta cgagtttcat
    57061 cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat gtccaatctg
    57121 ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag ggctctcttg
    57181 accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac aatattcgac
    57241 taaattacga gctgcatcta ctccacgagt acccagattg acagccatat tgatttcggc
    57301 ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg tttcaattgt
    57361 ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac tgtcttccca
    57421 acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt tcaattcttt
    57481 taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat gatctggctg
    57541 ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt acaaccgcgg
    57601 atcaactttg cctacacgct tgacactact aatcaacatt gtggcttcca taactgacct
    57661 cctttaattt atacatgaat aatacacctc gttgaaagcg ttgtcaataa tataaatgtt
    57721 aaacttactt attttttatc gattttaatt aaccaaaata ttaataatga ttaaaacaaa
    57781 taatttaata taacttttat taacaaatat gttaatatat aagaaaaacc ttttaagagg
    57841 tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta
    57901 gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt
    57961 tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg
    58021 aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg
    58081 ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat
    58141 cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga
    58201 tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg
    58261 atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa
    58321 atacagagga tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt
    58381 tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa
    58441 catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc
    58501 cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga
    58561 atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca
    58621 cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat
    58681 ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat
    58741 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
    58801 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
    58861 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
    58921 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
    58981 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
    59041 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
    59101 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
    59161 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
    59221 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
    59281 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
    59341 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
    59401 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
    59461 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
    59521 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
    59581 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
    59641 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
    59701 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
    59761 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
    59821 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
    59881 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
    59941 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
    60001 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
    60061 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
    60121 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
    60181 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
    60241 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
    60301 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
    60361 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
    60421 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
    60481 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
    60541 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
    60601 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
    60661 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
    60721 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta
    60781 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
    60841 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
    60901 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
    60961 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
    61021 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
    61081 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
    61141 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
    61201 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
    61261 aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat
    61321 gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga
    61381 ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt
    61441 ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc
    61501 ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt ggaaaggcga
    61561 attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca cacgttttta
    61621 aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg gcgagacatc
    61681 aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt accataaaga
    61741 tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc actacaatat
    61801 ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc actcctacat
    61861 ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag tcgatttctt
    61921 gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca tatgtgtcta
    61981 ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata gccgtattcc
    62041 caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta ctattcacag
    62101 gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag atccaatctt
    62161 aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac ctttaaagac
    62221 aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa aaaaatacaa
    62281 ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag gaggaaaaat
    62341 aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga gaaccttgcg
    62401 cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag gttatcggat
    62461 ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa caatggcatt
    62521 caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc agaaagcttt
    62581 aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc tcttgacaac
    62641 tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt
    62701 tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac aagagattcg
    62761 acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga atatggataa
    62821 agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc tcactcttta
    62881 cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc ataagaagtg
    62941 gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag gaatgatgta
    63001 cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa
    63061 agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt
    63121 acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt tatgctaact
    63181 tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata tattcatcgt
    63241 tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa caattgaaat
    63301 gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag aagctcgctg
    63361 atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata gctttgcaag
    63421 atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag ctctttaaaa
    63481 aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac aagtgattca
    63541 tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt ataacttagc
    63601 ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt caaataagta
    63661 agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg ttttcccttg
    63721 atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc atctagtgta
    63781 ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc ttcaagatac
    63841 cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta atcataatca
    63901 aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt ggtataaggc
    63961 agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt tgcattaggg
    64021 atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc atctacatat
    64081 ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag ctttgcgata
    64141 tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct tgtaaatagt
    64201 ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc gaaaaactga
    64261 tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc attggtattt
    64321 cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg gatcccttga
    64381 actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct acataagtat
    64441 tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt ctcatagcaa
    64501 ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa agtctcttat
    64561 tttaaattga atacattata atgatactgt aaatatatct ttattattag tgaat
//
LOCUS       DACCIQ010000006        63902 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCIQ010000006 DACCIQ010000000
VERSION     DACCIQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 63902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.006"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            55..1932
                     /gene="tdc"
                     /locus_tag="IVM62_000387"
     CDS             55..1932
                     /gene="tdc"
                     /locus_tag="IVM62_000387"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6844380.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2175..3596
                     /locus_tag="IVM62_000388"
     CDS             2175..3596
                     /locus_tag="IVM62_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6844381.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3705..5072
                     /gene="nhaC"
                     /locus_tag="IVM62_000389"
     CDS             3705..5072
                     /gene="nhaC"
                     /locus_tag="IVM62_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6844382.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5353..6372)
                     /locus_tag="IVM62_000390"
     CDS             complement(5353..6372)
                     /locus_tag="IVM62_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6844383.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(6394..7401)
                     /locus_tag="IVM62_000391"
     CDS             complement(6394..7401)
                     /locus_tag="IVM62_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6844384.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(7394..8059)
                     /gene="pgmB"
                     /locus_tag="IVM62_000392"
     CDS             complement(7394..8059)
                     /gene="pgmB"
                     /locus_tag="IVM62_000392"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6844385.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(8052..10346)
                     /locus_tag="IVM62_000393"
     CDS             complement(8052..10346)
                     /locus_tag="IVM62_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6844386.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10606..12771
                     /locus_tag="IVM62_000394"
     CDS             10606..12771
                     /locus_tag="IVM62_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6844387.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13075..13908
                     /locus_tag="IVM62_000395"
     CDS             13075..13908
                     /locus_tag="IVM62_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP6844388.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(14010..15710)
                     /locus_tag="IVM62_000396"
     CDS             complement(14010..15710)
                     /locus_tag="IVM62_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844389.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            16217..17377
                     /locus_tag="IVM62_000397"
     CDS             16217..17377
                     /locus_tag="IVM62_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6844390.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17393..17836
                     /locus_tag="IVM62_000398"
     CDS             17393..17836
                     /locus_tag="IVM62_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844391.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(18319..18849)
                     /locus_tag="IVM62_000399"
     CDS             complement(18319..18849)
                     /locus_tag="IVM62_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP6844392.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            18954..19505
                     /locus_tag="IVM62_000400"
     CDS             18954..19505
                     /locus_tag="IVM62_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6844393.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(19658..20131)
                     /locus_tag="IVM62_000401"
     CDS             complement(19658..20131)
                     /locus_tag="IVM62_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6844394.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            20269..21741
                     /locus_tag="IVM62_000402"
     CDS             20269..21741
                     /locus_tag="IVM62_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6844395.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            21738..22448
                     /locus_tag="IVM62_000403"
     CDS             21738..22448
                     /locus_tag="IVM62_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP6844396.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            22800..23084
                     /locus_tag="IVM62_000404"
     CDS             22800..23084
                     /locus_tag="IVM62_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP6844397.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(23365..23784)
                     /locus_tag="IVM62_000405"
     CDS             complement(23365..23784)
                     /locus_tag="IVM62_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP6844398.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(23786..24370)
                     /locus_tag="IVM62_000406"
     CDS             complement(23786..24370)
                     /locus_tag="IVM62_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP6844399.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(24417..25892)
                     /locus_tag="IVM62_000407"
     CDS             complement(24417..25892)
                     /locus_tag="IVM62_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP6844400.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            26073..26384
                     /locus_tag="IVM62_000408"
     CDS             26073..26384
                     /locus_tag="IVM62_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844401.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(26499..28835)
                     /locus_tag="IVM62_000409"
     CDS             complement(26499..28835)
                     /locus_tag="IVM62_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6844402.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            28977..29645
                     /locus_tag="IVM62_000410"
     CDS             28977..29645
                     /locus_tag="IVM62_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6844403.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29648..30691
                     /locus_tag="IVM62_000411"
     CDS             29648..30691
                     /locus_tag="IVM62_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6844404.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(30928..31809)
                     /locus_tag="IVM62_000412"
     CDS             complement(30928..31809)
                     /locus_tag="IVM62_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6844405.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            31968..32696
                     /locus_tag="IVM62_000413"
     CDS             31968..32696
                     /locus_tag="IVM62_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP6844406.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32852..33169
                     /locus_tag="IVM62_000414"
     CDS             32852..33169
                     /locus_tag="IVM62_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6844407.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            33162..33770
                     /locus_tag="IVM62_000415"
     CDS             33162..33770
                     /locus_tag="IVM62_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP6844408.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            33786..34616
                     /locus_tag="IVM62_000416"
     CDS             33786..34616
                     /locus_tag="IVM62_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP6844409.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            34754..35242
                     /locus_tag="IVM62_000417"
     CDS             34754..35242
                     /locus_tag="IVM62_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6844410.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            35293..36165
                     /locus_tag="IVM62_000418"
     CDS             35293..36165
                     /locus_tag="IVM62_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP6844411.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            36341..36703
                     /locus_tag="IVM62_000419"
     CDS             36341..36703
                     /locus_tag="IVM62_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6844412.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            36712..37464
                     /locus_tag="IVM62_000420"
     CDS             36712..37464
                     /locus_tag="IVM62_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844413.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            37478..39280
                     /locus_tag="IVM62_000421"
     CDS             37478..39280
                     /locus_tag="IVM62_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844414.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            39557..40597
                     /locus_tag="IVM62_000422"
     CDS             39557..40597
                     /locus_tag="IVM62_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6844415.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(40649..42766)
                     /locus_tag="IVM62_000423"
     CDS             complement(40649..42766)
                     /locus_tag="IVM62_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6844416.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(43123..44496)
                     /locus_tag="IVM62_000424"
     CDS             complement(43123..44496)
                     /locus_tag="IVM62_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6844417.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(44496..44891)
                     /gene="rbsD"
                     /locus_tag="IVM62_000425"
     CDS             complement(44496..44891)
                     /gene="rbsD"
                     /locus_tag="IVM62_000425"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP6844418.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(44919..45836)
                     /gene="rbsK"
                     /locus_tag="IVM62_000426"
     CDS             complement(44919..45836)
                     /gene="rbsK"
                     /locus_tag="IVM62_000426"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP6844419.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(45944..46957)
                     /locus_tag="IVM62_000427"
     CDS             complement(45944..46957)
                     /locus_tag="IVM62_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6844420.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(47038..47637)
                     /locus_tag="IVM62_000428"
     CDS             complement(47038..47637)
                     /locus_tag="IVM62_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6844421.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            47823..48524
                     /locus_tag="IVM62_000429"
     CDS             47823..48524
                     /locus_tag="IVM62_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844422.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            48534..51245
                     /locus_tag="IVM62_000430"
     CDS             48534..51245
                     /locus_tag="IVM62_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6844423.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            51642..53270
                     /gene="treC"
                     /locus_tag="IVM62_000431"
     CDS             51642..53270
                     /gene="treC"
                     /locus_tag="IVM62_000431"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP6844424.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(53330..54511)
                     /locus_tag="IVM62_000432"
     CDS             complement(53330..54511)
                     /locus_tag="IVM62_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6844425.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            54763..56286
                     /locus_tag="IVM62_000433"
     CDS             54763..56286
                     /locus_tag="IVM62_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6844426.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(56355..57083)
                     /locus_tag="IVM62_000434"
     CDS             complement(56355..57083)
                     /locus_tag="IVM62_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6844427.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(57096..58376)
                     /locus_tag="IVM62_000435"
     CDS             complement(57096..58376)
                     /locus_tag="IVM62_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6844428.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(58517..59119)
                     /locus_tag="IVM62_000436"
     CDS             complement(58517..59119)
                     /locus_tag="IVM62_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP6844429.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            59364..59570
                     /locus_tag="IVM62_000437"
     CDS             59364..59570
                     /locus_tag="IVM62_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844430.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            59882..60601
                     /locus_tag="IVM62_000438"
     CDS             59882..60601
                     /locus_tag="IVM62_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6844431.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(60682..61365)
                     /locus_tag="IVM62_000439"
     CDS             complement(60682..61365)
                     /locus_tag="IVM62_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP6844432.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            61583..63769
                     /locus_tag="IVM62_000440"
     CDS             61583..63769
                     /locus_tag="IVM62_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6844433.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        20362 a        11404 c        12977 g        19159 t
ORIGIN      
        1 aggtgtaatg tagggctcga caaaatttaa atattttttt aggagggttt cactatgagt
       61 gaatcattgt cgaaagatct aaacctaaat gccctattca ttggggacaa agctgagaac
      121 ggccaaatct ataaagcttt gttgaatgaa ttggtggatg aacatttagg ctggcgtcaa
      181 aactacatgc ctcaagacat gcctattatt acgccagaag aaaaaagcag tgctagtttt
      241 gaacacactg tgaataaaac aaaggatgtt ctatcagaaa tttcagcgcg gatgcgtacc
      301 cattccgttc cttggcataa tgccggacgt tactggggtc atatgaactc tgaaacattg
      361 atgccatctt tactagcgta caattttgct atgctatgga atggtaataa cgttgcctat
      421 gaatcttctc ctgcaacaag ccaaatggaa gaagaagtag gaatggaatt tgcaaaatta
      481 atgagctata aagatggctg gggccacatc gttgctgatg gttctttagc aaacttagaa
      541 ggactttggt atgcccgcaa catcaaatca ttgccgcttg caatgaaaga agtgacacct
      601 gaactagttg ctggaaaaag cgattgggaa ttaatgaact tgtcaacaga agaaatcatg
      661 aatctgttag acagcgtccc agaaaaaatc gacgaaatca aagctcactc agcacgtagc
      721 ggaaaacatt tagaaaaatt aggaaaatgg ttggttccac aaactaaaca ctattcatgg
      781 ctaaaagctg ccgacatcat cggtatcggt ttagaccaag tgattccagt gccagttgac
      841 cacaactatc gcatggacat caacgaactt gaaaaaatcg ttcgtggtct agcagctgaa
      901 aaaacaccaa tcctaggtgt agtcggtgtt gtaggatcaa cagaagaagg agcaatcgac
      961 ggtatcgata aaatcgttgc attgcgtcgc gtattagaaa aagacggtat ttacttctac
     1021 ttacacgtag atgctgctta tggcggatac ggccgtgcga ttttcttaga cgaagacaac
     1081 aacttcattc catttgaaga tttaaaagat gttcattaca aatacaatgt ctttacagaa
     1141 aacaaagatt atatcttaga agaagtacac agtgcatata aagcaatcga agaagcagaa
     1201 tctgtaacga ttgacccaca taaaatgggc tatgttccat attctgccgg tggtatcgtc
     1261 atcaaagata tccgcatgag agacgttatc tcttactttg cgacatatgt atttgaaaaa
     1321 ggcgcagata tcccagcatt acttggggct tatatcttgg aaggttcaaa agcaggggca
     1381 acagcagcca gcgtttgggc agcacaccat gtcttacctt tgaatgtcac aggatatggt
     1441 aaattaatgg gtgcatcaat cgaaggtgca catcgtttct acaacttctt gaatgacttg
     1501 tcatttaaag taggagacaa agaaatcgaa gttcatccat taacttatcc agatttcaat
     1561 atggtagact atgtattcaa agaaaaaggc aacgatgact tagttgcaat gaataaattg
     1621 aaccatgacg tatatgatta ttcttcatac gtgaaaggaa gcatttacgg taatgagttc
     1681 ttaacttcac atactgactt tgctatccca gactatggca acagcccatt acaatttgtg
     1741 aaccaactag gattctcaga tgaagaatgg aaccgtgccg gaaaagtaac tgtattgcgt
     1801 gccagcgtaa tgacaccata catgaacaaa gaagaacact ttgaagaata tgctgaaaaa
     1861 atcaaagcag ctcttcaaga aaaattggaa aaaatctacg cagaccaatt attggcaagc
     1921 gaagcaaaat aagcaaggta atcatgttag aaaacagctt gaatactaac tgtttctgac
     1981 gcatgtttgg atctgaagag caaggggaga cctaagctct tcgatccctt tgttttgagg
     2041 ttgtctttga agaaaccctt caagcaataa actcaaagaa tcccctcaaa tattcgctga
     2101 attgtttcgt ttattgctga aactacttca aagacaccgt tattttatcc aaaaaatcaa
     2161 ggagagtgtc aacggtgagt aaatctgaag taagtaaaat gggtcttggg acctttatcg
     2221 gtctgacaat ggcactgtgt gcaacggttc ggagtattcc tacgttagcc gcagtcggtt
     2281 ggacattgat tttttattca atttttgcag ttttgttttt tgccggtccg atatcaatgg
     2341 tttccggtga gctttccacg atgcttcctc aagaaggcgg tcctcagtta tgggtaaaaa
     2401 cagcactggg cagcaaatgg ggatttgtcg ttgcatggct attatgggtg caaatgttcc
     2461 caggtatggt aatggtcgct tctactttag gaccattgtt aggaaacaca tttggtaatg
     2521 tagaacttgg caataatcac tggttcgttt taggttgtat tttagttatc tactggatca
     2581 tcacgatctt gaacttgaaa ttcgatatgg caaaagtcgg cgggaacatc ggtgtatggc
     2641 taggggtata tatcccagta gtcatcatgt tcgttttagg tgttttggca gccttcaaag
     2701 tcggactagt ctcaaacggc tatctaggtg acttttcatg gtcaaaagct ttccctgact
     2761 tggaacatat tgattcattg aaatacttag ctggtatcac atttattttt gttgggatcg
     2821 aaatgagttc tgtttacatg cctcgtttga aagacgcaac aaaaaattat acaaaaggcg
     2881 tatttatcgc tttgatcgga ttagttttat tgaatgtcat caatgcgatg cttgtagcaa
     2941 atgtcgtacc agatggaaaa atggaattag caaatatcac gcagcctatc ttgattgact
     3001 gtcaaatcct aggcttacca gaagtgatcg gaaatatctt cagcttcatg gtatttattg
     3061 gtgtgctttt acaattatca gcttgggtta ctggaccatc aaaaacaatc atccaagtag
     3121 ctcgtgaagg attccttcca ccaaaattcg gattccataa agaaaacaaa tacggcgtat
     3181 ctagaaatgt cgttctaaca caatcaatcg ttatttcatt atttgctttg ctttatggtg
     3241 taatggatga cgtaagtgct gtattcttga cacttacaaa tgctacaact gttatttatt
     3301 gtatcgtgta cattttgatt gctgtttcat tgcttaaaat gcgtaagaaa catccagaat
     3361 tcgaacgtcc ttaccgaatt ggtaaaaacg gaaacggatt agcttgggta gtcagctgca
     3421 tgctgatctt ctctatcatc gttgtcgtat ttgctacatt aggtacagcg acattgagtg
     3481 atgcattatt agtagcagct atcacagtgg tcatgttcgt tattccattg atcatcaatc
     3541 acttcaagaa agattcttgg gggattgagg tagaaaaaag tttagaagaa aaataaaaaa
     3601 ataagtcttt cagccgacgt tttgtcgctg atttagtagg aggtcctgcc aagtgaatag
     3661 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
     3721 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
     3781 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
     3841 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
     3901 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
     3961 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
     4021 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
     4081 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
     4141 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
     4201 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
     4261 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
     4321 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
     4381 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
     4441 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
     4501 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
     4561 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
     4621 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     4681 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     4741 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     4801 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     4861 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     4921 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     4981 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     5041 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     5101 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttttaaa tttttgtgaa
     5161 tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt
     5221 gagacaaaag tgttctttac ttttgtccca cgctcttttt tacagataaa caatgtgtta
     5281 gaagtagtat cttcttttac ataaaaagct gaccatagga taaatggtca gcgtatttag
     5341 tgagtcagct atttagtatg ggttttctaa aggaacgact gtctctcttt tgatcaagcg
     5401 atgagggatg accaaacgta cgccactgct caattcttca tggatcaatt cgcgcagctt
     5461 ttgcatagcg acacgtccta attcggagat atcgatatcg atacttgtaa ggtaagggtg
     5521 tgttaatgtt gagaagatcg agttgttgaa gctaatgacg gagagtgtct ctggaatgac
     5581 atagccatac atttgcgcca actgcatcgt gcgaagggcg aagatatcgt caatgaccac
     5641 caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag gacagataat cctccggatg
     5701 ctctagtact acactggata acgcaggaag accagccatc atcattgctt tttgataacc
     5761 gaaatatcgt tcgaaataca aactttcatg agtcgtgttt gtcacaaaca ggatattctt
     5821 atgtccattc tggatcaaaa attctgttgc ttgtttacct agaagctggt tatcattatc
     5881 tacataaacg atttcgttct catgatgata aggctgtccg atcaaagtga aagggatgtg
     5941 gttttggtat aaataatcaa tgacagtatc ttgactatct gaatacacta gaataaaacc
     6001 atctacttgt ttttgtaaat gcatacgctg gacgttctct aacagcaatt caaaattttt
     6061 agcagtagcg attgctgtag tcatatcata attccgcgct tcttcgttga ttgcttccat
     6121 gatctcaaga taaaatgggt taccgatccg ctcttttgaa tcaagagggg gtaagattac
     6181 gccgatggca ctggaaatgc gttttcctag attgcgggca gtcatgttag gaacataacc
     6241 tagttcatcc ataatttttc ggactttctt ctttgttgtc tcggaaatac taggatgatc
     6301 gttgatgaca cgagagacgg tcgaggtggc aacaccggct tttttcgcca catcttttac
     6361 ggtaatagcc atgataaaaa cacctttctc ttgttattgt gcagagaacc gataaatcgt
     6421 ctgactgatc cgttcttgac caggatggag gttgatcgat ccaaatttgg ggaaatgaac
     6481 gatatcaggg atttcttgcg gttcgattgc taatccgtag tttgaatgca tctgttttcc
     6541 attgacagag aaatcagctt cgaatccagt cgtagaaaat aataccatac ttttattaga
     6601 agttgctata gtcatttggc gaccagaatg cttctcgaat aattgtaagg aagttttgga
     6661 aagcctagga tgatgcaaaa gaaatacatc atctagtcca tctggataac atgcaaggat
     6721 ctcttgcagt gttggggatt ttctaaagtc gattggcagt ttttcttttt ttgtccaatt
     6781 tccagtaggc agtttttctt gatctaattc aagcacacca tctaaaaaga ccgttaactg
     6841 atgttcatag atgtctcttg ttccatttcc tgaaaggttg aagtaggcat ggttggtcgg
     6901 attgcagatg gtttcaacaa ggctactagc agaggttgtc atctctaatt cgttttttgt
     6961 taaacggtaa gtgatcgtag cagtgattgg tcctggatag gtagaaaatt catcttttaa
     7021 gtaaaagacc acacctattg atgatggagt tttgaaaact tctacttccc aaaattggaa
     7081 agaccagcct ttcgtgccac cgtggatatg gtgtgatccg cagtttttct ccaatctatg
     7141 ctcaccccaa gaagcatccc ggatccttcc ggctacaggt ccgacagttg ctccaaaaaa
     7201 gcttttgtct gttagaacat cttcgaaacg atcgtaagac aataaaacat tttctgattt
     7261 tccttctttg tctggtgcga agatttggtg caggcgggca ccgtaattga gaagaacgac
     7321 ttctaagtag tcgttcttca atgtgatttt ctttatttca gtctctggaa ctggtacgac
     7381 ggagatctct ggattatcca tggtcatgcc acttttcttt caagtaatcg atcgtaagaa
     7441 aagtcgtatc agggacaagc gggatctcct ctccaaggtc ttcagcacgt cccacgccaa
     7501 ttggttgagc gccacttgag aggatggctt ggataccggc ttgcgcgtct tcgatcccga
     7561 tacattcttc tgcttttagt ccgacagctt ttgctgcaag gaggaagata tctggcgcag
     7621 gttttccgtt tgttgcttct gccgggtcgg caatagcatc aaagtaaggt gtaagaccca
     7681 ttttttcaag taggaatggc ccgtttttac ttgcagaagc aagagcaatc ttgatttttt
     7741 cttcttttaa agaatccagt aaaggcacga ccccggggaa gacatctttt ggttcgatcg
     7801 tctggatcat ctctaaataa taatcgtttt ttcttttcgc taattctgca aattcttcct
     7861 ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa agagagtgag tcttctcggc
     7921 tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt aatgccgatt tcctgaccaa
     7981 gctttcgcca agcacgataa tggaattcag cagtatcggt aatgacaccg tctaaatcaa
     8041 acaatacccc tttaaacatt tgcttcctcc tttcttagcg ttatttctag acgatccgct
     8101 aaagaataag actgttcata gacgtaaatt tctagaggag aaccttctaa taactcgaaa
     8161 gtaacttgtt catctgtgac tgagatgaac aataaacgtc cgcgataatt gatatggaaa
     8221 gaataagctt tccaagtttt tggcagaaat ggtgcgaatc tcaactgatc atgggcagtt
     8281 ttcatttgag caaatccttg gacaatcgac aaccagctac cagtcatgga agtgatgtgc
     8341 aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca agcgagctgt tcgctggtac
     8401 atttctacag ctttgctttc cattcctaat tctgctgcta aaatcgaatg gatacttggc
     8461 gaaagggaag actcatggac agtcattggt tcatagaaat caaagttttt ttgcttttct
     8521 tcttttgtaa aagtatcatt gaagaaataa atcccctgta gtacatctgc ctgcttgatg
     8581 aagcaagatc taaggatttt gtcccaagac caatgctgat tcagaggaag atcgcttgtg
     8641 ggaatatcgg atacaggcat gagatcttta tctaagaatg tgtcatgctg gacaaagatt
     8701 cctaattctt cgtcaactgg gaagtacatg ttctctgcga tatctttcca tttggctagt
     8761 tcttcttcgc tgatagtgat agcagcttct ttttgataag ttaaataatt ttctcgcgtg
     8821 taacgcaaga cccaagctgc gatcgtattt gtataccaat tgttattgat attgttctca
     8881 tattcgtttg ggccggtcac accgtggatc atgtatttat ttgctcgttt tgaaaaatgg
     8941 acacggtctg cccagaagcg tgcgatttct gctaaaactt ccagtccttc atttttcaaa
     9001 tattccgtat cgccggtgta attcgtataa ttgtagattg cataggcaat cgcgccattt
     9061 cggtgaatct cctcgaatgt gatttcccac tcgttatggc attcgacacc agtgaaggtg
     9121 accataggat aaagcgcgcc ttttaatcct tgttgctgag cgttgtggat tgcttgcggc
     9181 agttgattat gccgatattt cagcagattc ttagttactt caggtttggc taaagcaaga
     9241 taaagaggga cagcatacgc ttctgtatcc cagtaagtgg ctccgccgta tttttctcct
     9301 gtgaatcctt tggggccgat attcaatcgt tcatcttcgc cgtagtaagt agagaatagt
     9361 tggaacaaat tgaatcgaat accttgttgt gcttcttcgt ctccttcgat gaccacatcg
     9421 gcaagcttcc atcgttctgc ccaagccgct gtttgctctt cttttgcttg gatataattg
     9481 tttgtctggt tcgttaattc tgagacagct ttttctgctt gctgcgcttc tggaatatct
     9541 ctactagtaa gaacgataat ttctttttcc actgaccatg tctcaccttg agataattct
     9601 gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat agtcagggat gactgatttt
     9661 ccattaacga aatggcgcat tgctgcagta acagtaaatt gttcaatccc aaagttgttt
     9721 ggaatagttt tagtcgtcaa atagccgatt tcttctatag ttccacgatc aatctcattc
     9781 caaaaatgtt catcatagtt gctgtcttcg ttttgaacat ttccatccaa ttttgatacg
     9841 agtttgactg ttccgctacc ttcaagcatt tctatagtta aatagatata agctgattct
     9901 tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta ctttattagt agaagtagtg
     9961 attgtgaatg tacgagataa tacaccattt ttcatatcta attcccagta aaagtcttct
    10021 ggttccaaag aagccaagtc gatcagctga tcgttgatcg aaatatctat cgcgataaag
    10081 ttcaaggcgt tgatcacttt tccaaagtat tctggataac catttttcca ccagccgaca
    10141 cgtgtcttat cgggatacca gacacccgcc aaataagtac cttgatggtg gtcccctgag
    10201 taagtttctt caaagttccc gcgcatcccc atataaccgt ttccaatact tgttaaagat
    10261 tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc tgattttcca tggatcaata
    10321 gcaaatagtc gtttgatctg tttcattttt ctcctcctaa tgagctgttt tatacgttca
    10381 taataaacgc aaccgattgc acaagtcaat agacaaaaga aaatatttca tagaaaaaag
    10441 cttaaaaaaa taaattttta ggagaaaatt atagaaggga acacacgtat tataggtttt
    10501 atagaatggt atagacagaa aaataaaaaa taaagcattt acaaaaaaga aaacgcacgc
    10561 tataatacgt ttaacgaaac cgattgcgta aaggagctaa acggaatgaa aaagatattt
    10621 agttttgagt tttggcaaaa atttggaaag gctttaatgg ttgtggtagc agttatgccg
    10681 gcagctgggt tgatgatcag tatagggaaa tcaattccct tgatcaatcc tgattggacg
    10741 ccgcttgtca caacgggcgg agtaattgaa aatattggtt gggcaattat tggtaatctt
    10801 catattttat ttgctttagc aataggagga agttgggcaa aagaaagagc aggcggagca
    10861 tttgcagctg ggatctcatt tattttaatc aatcggatca ctggggctat ctttggtgtc
    10921 acaagtgaca tgcttgcgaa tgaagatgca tttacccaca ctctgttcgg tacgaagatc
    10981 atggttaaag gattttttac aagtgtattg gaagcaccag ctttgaatat gggagtattc
    11041 gtagggatca tcgctggatt tgtcggggca atggcttata ataagtacta taattataga
    11101 aaattgccgg atgctttgtc atttttcaat ggaaagcgct ttgttccgtt tgttgttatc
    11161 ttatggtcaa caatcgtggc attgattctt gctgtggtgt ggccaaacgt acaggctgga
    11221 atcaataatt ttggtttatg gattgcagaa tcacaagaaa gcgcaccgat tctagcaccg
    11281 tttttatttg gaacattaga acgtttacta ttgccatttg gattgcatca tatgctgacg
    11341 attcctatca actatacaca gcttggcgga tcgtatgaga ttttatcagg cgcacaggcg
    11401 gggacacaag tatttgggca agatccgtta tggttggctt gggccactga tttagtgaat
    11461 cttaaaggtg caggggatat gtcgcaatat gagtttgtgt tgcataactg gacgccggct
    11521 cgtttcaaag taggacaaat gattggttct tcaggtatct tgatgggaat ggcttttgcg
    11581 atgtatcgta atgtcgatcc tgataagaaa gcaagatata aatcaatgta tttttcagca
    11641 gcgttggctg tctttctaac aggtgtaaca gaaccattgg agttcatgtt tatgtttgct
    11701 gctgtgccat tatatgtcat ttattcagtg atccaagggg ctgcatttgc gatggcagat
    11761 atcttgccat tgcgggtcca ttcatttggg aatatcgaat tgctgactcg gacaccgtta
    11821 gctatcaaag cgggtctagg tggagatctt atcaacttcg tgttgtgtgt gatcgctttt
    11881 ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa aattcaactt tgctacaccg
    11941 ggacgtaacg gaaattacga caatgacagt gaagaaaccg ttgtatctaa cagcggaaca
    12001 ggaacagcag atcagcaagt ggtccagatc attcatttgc tgggaggaaa agaaaatatc
    12061 aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg taaatgatcg agaaaaagta
    12121 gggactgaag aagcctggaa acgagcaggg gcgatgggat tgatcgtcaa agacaatgga
    12181 gtccaagcag tttatggacc aaaagcagat gtcttgaaat cagatatcga agatctgctg
    12241 caatcaggag cagagattcc agagccagaa atggtttcta caggaaatgg acaagcaggt
    12301 agtaagcaat acttaggtat agaacaagaa ctagtttccg cagcttctgg agaagtgatt
    12361 cctttatctg aagtaaaaga tccggtattc tcacaaaaaa tgatgggtga cggctttgct
    12421 gttattccaa catcaagaga agttgttgcg ccgatcgctg gaaaagtgac aagtattttc
    12481 cctagcaaac atgcaattgg gctggaaacg aaagatggta tcgaagtttt gatccatatg
    12541 ggaatcgata cagtccaaat gaatcagcca gcttttgaga ttgctgtcaa agaaggccaa
    12601 gaagtaggaa caggaacgaa attagctgag atggatcttg atgtgatcca aaacgaagaa
    12661 aaagatccaa cgatcatgat cgtatttaca aacgataaag tagaagaagt agtgatcaag
    12721 caattaggta cagtgacagc tggtaaagtg atcggctcca ttaaaatgta aggagaggtt
    12781 gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa acaaaaatag ctcctcctat
    12841 ttttcgtttg gttgggctta ttaccgagaa gcagcagttt gaatactgtt tagtggagag
    12901 gtcgtgaaaa agctgccctg caccaagtga taaaaacagc caaacaaaaa atagctcctg
    12961 gtccattgag cttattactg aggagttagc aggagaacac tatttgctgg agaggttgtg
    13021 acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga caaggagaaa accaatgaaa
    13081 ttcatgacac tcaacacgca tagctggctt gagccagaac cagagaaaaa attgcaagaa
    13141 ttagcagata agatcctgtt agaagattat gaagtgattg ctttacaaga aatcaaccaa
    13201 ttactggaat ctgaagaagt agaacctgcg aagctaatga agttctgtcc ggtaaagaat
    13261 caagtaccta tccgtaaaga taattttgcc taccgtttag ttcagctttt aaaggaacac
    13321 ggaaaagagt atttttggag ttgggaaatg agccacatcg gttatgataa atatgaagaa
    13381 ggaaatgcac tgcttacgaa aaaaacattg gaaagtcagg tccttaccgt ctcccaattt
    13441 caaaagaaag aagattatca gacacgcaag atcttgattg ggaaaacaaa gatagatgat
    13501 caggacattt ttgtctcgag ctgccacttt tcttggtgga cagataaaaa aagtggcttt
    13561 tattttgagt ggaaaaactt ggaaaactac tttttggaaa ctcgcgtacc actttttttc
    13621 ctaggtgatt tcaataatcc agtagactct caaggttatt atactgtaag agaaagttgc
    13681 cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag gaaaagcagc tactgtggaa
    13741 aagaaaatag atggttggga acaaaataca gaaaaattgc gtatagattt tattttcgtc
    13801 cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg atgggatcga tagtccaatc
    13861 atcagtgatc actacggggt ggaaatcgaa ttagatgtga acgaataaaa aatataacat
    13921 ataacataaa aaaactgatt aaaataaaac tctccaaacg attataccgt ttggagagtt
    13981 ttttgatcgg atgtatttat cccttttgcc taagcacgta aaagcttgta tctatgataa
    14041 ataaataaac atagcaaggt aaaccagcca atcaaagtaa gagaaagaat gaaatagaaa
    14101 tagattggcg tttgataatg aagcgtgagc tcgttgattc ctttttgctg cttcacgatt
    14161 ggggtcccga tatctgttac ttcataatca gtcaatgttt ttccattttg ctgtagcaca
    14221 gtccgatcgt agacgataac agggatatga acagttttgt ccccagtgtt tttccacttg
    14281 attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg tttcgttttc tagtatttct
    14341 tcgttatacc ggtcgtattt attgtctttt gtctccttat agattggcag atagtcaggt
    14401 gtggtttttt ggaaaacgaa caagctggta gacatatctt tgctgaagaa tgttttcctt
    14461 gcttcttccg gcgtatcgaa tagataagta tgtcgactga caaatgtttc attttcccac
    14521 ttttccagat gctggtataa gttctggatc gtctgtgctg aaaaaataag aatgccaaat
    14581 atacttaaaa gacggaacca cttttgttca gaccactggt tcagtgaaag ggcaaggtaa
    14641 agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg gaaactggat caaactaata
    14701 aaaggcacat ttttttcgat caatgtcgtc catggaatca agctagtaga aagaaaaaag
    14761 aaaacaagac ttgtgtacaa cacgattttt tggaataagg tgttcttttg ttgaaagaaa
    14821 ataaaaagca aaaaacctga taatatcaat ataggaaagg ccttagggta atggagccaa
    14881 taactgccat ccagatctat cgccttttga ctcatttctc tgttaacaaa gggaggaaga
    14941 atcttattcc cactgtaaac aatagctaat cctgcccaaa tatttgctgt cagaacaaaa
    15001 taaagcaaga ccgacttaat cagttgaagc agtgcttttc ctttttcagt ctggttaaaa
    15061 aagtaataaa gataaaaagg gatatacatc aaagctaaca tgatcgaaga cagtaaatgg
    15121 atctgtgtca taagtcccac tgagacagcg actttgataa cagggaactt tttatgaagg
    15181 acgaaatcag caatagggat catacataaa ggataaaaac aagtccccca gctagtgaat
    15241 ccttggttga tagaccagta ttgaatagaa tacgtactca tgaataaaac ggctactggt
    15301 acacttaccc aatttcgaac attgacatat ctcagtaaaa tcagcataga aaatcctgaa
    15361 ataagataaa gaatgaaatt tgaaagaatc tgataatgaa accagctacc tgcaagcaac
    15421 acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa cggcattcac gatcctgcca
    15481 ctttgctgga atccataaag ggaaggaaaa taatggaaat ccccatattt aatttgttgc
    15541 gcggcatcat agaaccgatt gtaatggaat ggggcatcag cacccaaaat tagtttatgt
    15601 gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa aaattgctaa aaatgggcca
    15661 tattttttca attgtcgaac tcctttgcaa actttagata aattcatcat atatcctatg
    15721 cgagaggtag gcaagaaatt agaaaattca tgaaaatatt tatgaatttt acaagaaaaa
    15781 cataagtgaa agttctaagg agagaggatg cgacataagt tttgttgcat ctccttgttt
    15841 tgaaacagaa tttttcttaa tactcgaaac aaagtagctt tttcatgacc tctcggtaaa
    15901 cggtgttcaa tcagctgctt ctcggtaata agctcactag cctagaaaat atgagaagct
    15961 attttctgac tagcgctctt attactcaaa gcataacgca gttttcacaa cctcttcctt
    16021 aaacggtgtt caaaagcttg catctgcggt aataagccca acccaacaaa aaatatgagt
    16081 agctattttt ttgggctgta cttattactt gatgcaggac agctttttca caacctcatc
    16141 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
    16201 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
    16261 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
    16321 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
    16381 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
    16441 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
    16501 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
    16561 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
    16621 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
    16681 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
    16741 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
    16801 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
    16861 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
    16921 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
    16981 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
    17041 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
    17101 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
    17161 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
    17221 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
    17281 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
    17341 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
    17401 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
    17461 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
    17521 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
    17581 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
    17641 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
    17701 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
    17761 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
    17821 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
    17881 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
    17941 ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
    18001 tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg atttcacaac
    18061 ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta gcctagaaaa
    18121 tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc tgatttcaca
    18181 acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    18241 acatgaggaa ctatttttcc gtttctttct ctaatactca ggtcagaacg ctgatttcac
    18301 aacctctctt tcacaacctt attttactcg tttataattt tcatcgaagt attttccgct
    18361 acggatatca tctggcattc catcgtatgg ttcttcgcta ataacatctt cattgtctat
    18421 acctgcatca cccatataag tgatcgtagc aaattcagga acaagtaatt ttggataagt
    18481 ttcatatttt tcacgagctg cttccatttc tgcgatatgt tcgttttgga aagtaacagt
    18541 gatttctgga tgttcttcaa cccatttagg gaaaaagatc attccgtgaa gtttcttttc
    18601 atttggcata aagtccaatg ccacgtctgt ccctgttggc tctgtccaag tgattttgaa
    18661 tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct tttacccaac ggcctttgac
    18721 cattccgcca tggatacggt aatcaacagt atggtcattt ttggcatacc attcatattc
    18781 ccaaccattg tcatacgtat agataaagtg agttccaata aaatcttcca attctttaaa
    18841 ttctttcatg tttaagcatc tccttataaa ttaaaacatg taataaacta catgttttcg
    18901 attactcatt tatgatagaa tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa
    18961 gaaaaaatac acttcgatat attttgttag ggttattaag taagaagaag atgacaggat
    19021 atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt ttggcaagca aaacatagtc
    19081 agatttatcc tgaacttgca aaaatggaag agcaaggaat cattcagcat caagtagaga
    19141 tcacaggtga aaaattggaa aagaaagttt atgaattaac agaagaaggg aaggaacaat
    19201 tggcagagtg gattcataca cccaccaatg aactaccggt taatcgagac gaatttgttc
    19261 tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt agtagaaatt attcagcagc
    19321 agcgtgacct tcatgaagaa aaacgacttc atctattaga gcgaaaaaaa acgatctttc
    19381 ctactgaaaa agaaaaacat gaaaattacg gacactacct gattttagcg catgcgatca
    19441 atcgggaaac agaatatgca aattggctaa cagatgtatt agaagaaagt aaccgaaaaa
    19501 aataaaaaaa tatccgaaca gtgatggaga attcctttct atatgaaaga aacgcagatc
    19561 accgttcgga tattcttatg ttcaaacagg ccttttttca agtatttatg tagcatctac
    19621 ttattaaaaa gcttggtact taggagatta gtggcgttta gagttctttt aacaactgtt
    19681 cgtagtcagc tttttcatcg acaggaagga gcttgtaagt taattcgtct gtctcgttgt
    19741 catgaatgat gtacatctta gcattctctt ctttgaagtt accgatttgt aaaatcgtat
    19801 agcgttcgtc gatcaattcg atatctttga ttgcttttga aaaaacttta cggacatggt
    19861 gcattttctt ttggcggatt tctgcctttt ctgcagcttt tgacaaagta aatccgtcat
    19921 ctaaatattt tttgatcagt tctactttta tgactgtagg taaacgatat tttctaggac
    19981 aattcgcatc agcttcgatc gaacgaatga atcccttttg ttcccaatac cttaattgac
    20041 gtggagacac acctacgata tcacttaatt cactgatgcc aacaaccaat tgatcatttt
    20101 ccaacaaacg tttaagggaa gccccaacca tttttgtcac tccttttgtc ctcttgcttc
    20161 ccttaattat atccgtatta acttatgtgt caagtaggtt gacaaatagc gaatttagga
    20221 ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac
    20281 caacccgtag atatttacgg aaaaccatat aatcggacac ttttagttgt tgtcttatta
    20341 attggtacat tttgtaccgt tttgaatcag accttattga cgacagcttt tcctacattg
    20401 atgaaagctt tcgatatttc agcgtctgat gtgcaatggt taacgactgg tttcttgcta
    20461 gtcaacggga tcatgatccc aataacagca tggctgatca ataaattcag ttcaagaaat
    20521 ttatatcttt ctgccatgac gatctttttg atcggaacga ttacttgctt tacagcacct
    20581 aattttagta cattactaat cggtcgtttg atccaagctt gcggtgtggg agtatcgatg
    20641 ccattattgc aaaatatcat gctatctatt ttcccgccag aaaaaagagg atcagcaatg
    20701 gggatgtcag ggatcgttat tggacttgct cctgctttag gccctacatt atctggttat
    20761 atcattgata actaccattg gcgtgacttg tttggtatgg tactgccaat cgttgttctt
    20821 gtcttggttt tagcattctt cctaatgaaa agtgttattc cattgtcaaa tcctagcatt
    20881 gatattttat ccgctatttt atcaacgatt ggattcggta gtttacttta cggattttct
    20941 agtgtaggag atgacggctg gggaagcttg aaagtaattg gtttcttagt tgttggtgtg
    21001 attttcatcg ccttgttctc atggcgtcaa ttacatttgg aacatccatt cttggaactt
    21061 cgtgtattca aatcatctac attcacgatt gcagctgttt tagctggtgt aacaaatatg
    21121 gcgatggtag gggcagaaat ggtcgtacca ttgtatattc aaaatattcg tggagaatca
    21181 gcattccatt ctggcttgat gttgttgccg ggtgcattga tcatgggggc tatgatgcct
    21241 gtgacgggtg cgattttcga taaatacggc gcgaaacgtt tagccatcac agggatgttc
    21301 attttgacag cggcaactgc accatttgct ttcttgacaa aaacgactcc agttttatat
    21361 atcgtgatcc tttatgcgat ccgtatgttt gggatctcca tggtcatgat gccggtaacg
    21421 acttctggta tgaatgcatt gccaaataac ttgatcagtc atgggacagc ggtaaataat
    21481 acattccgtc aaatcgcaag ctcgattggt acagctattt tgatcagtgt gttgacgaat
    21541 gtgacaaaag caaatatccc aagcaaatct gtattggata atacgccatt agcttataaa
    21601 gatcaggcaa tcaatgccac actgtcaggt taccatgccg cgtttatcgt agcgatcatc
    21661 tttgggattc ttggctttat tattgccttt ttattgaaga aaaaagaacc tgttctaatg
    21721 aaagcggggg ataatgcatg attattggag ttttgattgt cagcttgctt gcttttgctt
    21781 taacgaacgt gttcgctaaa aaaacatggc aaaccttttt atctttgatt ttcggactaa
    21841 tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa
    21901 aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc tgattcccaa ggagcagata
    21961 tgcttttgta taaagcttta gggaatggaa aagaaaaagt gtatctttac cgaacaaatg
    22021 aaaaacaaga aaaaccaaaa gcaactggta cggataacga aacaaataaa gtagaaaaga
    22081 cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt
    22141 ataaattctg gttcaatatt gctgataaca atcacgaata tgacagccga gtgaatacat
    22201 tcaaaatccc agagacatgg gtagaattaa gtacggacca agcagccaag ttagctgaat
    22261 tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa agcttatgtt caagacggta
    22321 tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc agagcaagaa caacgtacga
    22381 aagccttagc tgctgaattc caacaacaag cattcgcaaa attagtgaaa gaagcaaaag
    22441 gcgaataata ttttgaacac catgtaggag gttgtgaaaa agctgctctg catcaagcag
    22501 taagaacaat cagataaaaa tagcactcca tatttttcat ctgattgggc ttagtgccgt
    22561 agatgcaagc ttttgaacac cgtttaagga agaggttgtg agcctgccgt ttaactctga
    22621 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgactattg tgacttgtga
    22681 ccgaggagtt ggcaggagaa caccgtttat tcgaaaaaag gaactgcgac aagacttttg
    22741 tcacagttcc ttcttttttt tgctatgcta tactagatgt aaaaaatagg agaaataaaa
    22801 tgagggaaat ttattctttt gaatcaaaaa tctatgcttc cgaagtcggc aagggcgggg
    22861 catatattat ttttccgtat gatataagaa aagaattcgg tgctggaaga gttaaagtcc
    22921 aagcaacttt tgacaatgtg ccgtatgaag gcagtatcgt gaatatgggc gtgaaaaatc
    22981 cagatggttc agtctgctac atccttggac tacgaaaaga tattcgtaaa aacatcggaa
    23041 aagaaatcgg tgatattgta gcagttacag tgaagcaaaa ataaaagagg ttgtgacaaa
    23101 agacgtgtca caacctcttg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca
    23161 aatttatgca aaaatgtgga aacccatgtt tacaaaattt ttcttactac tcgaaacgaa
    23221 gcggctttat cacaacctct tgataaacgg cgttcaatca gctgtccctc ggtattagct
    23281 caaaaacggc aaaatatgag aagctatttt tccatttttt tctctcatac tcaggtcata
    23341 acgctgattt cacaacctct tctttcataa tggtttttca ccatctttat aataaactgg
    23401 ttctttgata tggaaatcta ttttttccaa ccaatctaaa atcttgtaac ctaactcttc
    23461 tgacatcgga aaagaggcgt tgatttgtgc attggagata tggtcaacat ggataccgga
    23521 agtgatcgta caacttcccg gaacggcagt tttgatgatc acagctattt tttcggctaa
    23581 gacatcgtct ttatggaacc gaccatcgtg gctagggaaa cgtacagttt gcatctctgt
    23641 atcacgagaa aatgtagtga ctgttccgat atgtggtgta tctccgcctg ttaaagtgat
    23701 caataagtct ttaccgatta ctttaacagc agcttggatc gtgaaaccta gtttggttac
    23761 ttcaactatc gtttcttttt ccatattaat caccctgcca acgttttgaa tgctcattta
    23821 caatttcaaa ttgatccaaa agtttcattg tatggtgatt aacaagatct tggatcgttt
    23881 gaggctgatt ataaaaagca ggaattggtg gtatgatttg cacacctagt cgtgctaatt
    23941 tagtcaagtt ttccaagtga atggcattta atggcgtttc acgaggaaca atgactaact
    24001 ttcgttgctc cttcattgtt acatcagcag cacgtgcaat caggttgtcg cctaaaccaa
    24061 cagagatacc tgcgactgtt ttcatacttg ccggtacgat gatcattcca tcggtcaaaa
    24121 atgatccgct ggcgacagca gcacctaaat ctttgtttga ataatggtaa tcaaacaggc
    24181 tttcgaattc agttagtgat aagtcggatt ccagctttaa gttctgtttt gcccatggac
    24241 tgatgattcc gtgtgtttct atcatcggat attcttttag tttcttcact aggttgatgg
    24301 cataaatggt gcctgaagcg ccacttacac caatcacgat ttttttcttc cgttgtgcca
    24361 attgttccat tttccgcact cctttttatg atgtcaaaac agttgcagat cattcattat
    24421 ttcaaatatt tttcccaatc ttctacttct tggaattttg cacgtacaaa atgttctttc
    24481 atatcgaaag gaactgtacc gtcaaagata gtttttgcac ttatcccacg gaaacgaata
    24541 gagccaggat tatattctgg taattctgat gggtccaatg gatggttcct cattcctggg
    24601 atcacgatga tatctttatc tccttggaaa cgagtgttga aagtccaaac tacatcgttc
    24661 atgtcgaaaa tatcaacatc ttcatcgact aaaatgactg tcttcaattc tttgaaggct
    24721 gataaagcaa gaatagctgc ttgacgctga ataccttcat ccgcttcgtt ttctttacgg
    24781 atttgcatga tggtcatcaa tttaccacca cctgcaggag gattgtagac atttactact
    24841 tttcctggaa tagctttatc gaccaaggca agaatacttg cttctgtagg aataccggcc
    24901 atagatacgt gttcttcact gggaccaatc gttgtttgca tgattggatt gtcttttcgg
    24961 tgagtaactg ctttgacttt cactacttgt acagctggat tagcatcccc agtatagcca
    25021 ggaaattctg gcattgcttt tcctgagttc gtgttgatat cttcttgcat tgtttcattt
    25081 ggcatgattt ctgcttcaat gatgaattca gcacgagcaa tacccacttc atcgactgct
    25141 ataccatcaa cgatttgaat aggttcgtta cgcaaagctc ctgcacacca tagttcattg
    25201 tagccaagtg gagtggtagg tggttcgaaa gtcgtgccaa tggcaattgc tggatctaaa
    25261 ccaatattga tagtgatagg cattggttta ttcaaggctt cgaattgttt ttggaagtga
    25321 ccaatgtggc gtccgccagg catgatgtac ataccgatcg tatctttatc ttctagaacc
    25381 atacgatgga tcgttacatc gctcattgtt ttttctggat ctgatcctaa gactacaccc
    25441 attgtgatgt atggaccagc atcttcagga gtatttgtag gtgcaggcaa aatgttgcga
    25501 atatcgaagt ctgcatcggt agctagatga acgacttctt gtgctggagc gtctgctttg
    25561 tctactttca caggtttgat tggattttct acagcgtctt tcaagaaacg accaagtgtt
    25621 cgataatcat ggtgcaaaat ttcacctaca cgtttacgac tagccattgt tccgatgttc
    25681 acacgtgtgc cggggaaacc tttgatattg ttgaaagtca tagctggtcc ttcttgagtc
    25741 ggacgtttta ctgtaccgcc agcaccgata taacggtaaa caccagataa ttctgcttca
    25801 ggatcgattt caatatctgt ttcgtgatat tgacctggaa gtgtttttag ttcttcgatg
    25861 acttttctta aatcatacgg ttgatctgtc atgagagatt cctccttagt tgtctttacc
    25921 tatctatagt aaaaggtgaa aaagaggaaa acaattcaac ttgttcggta aagtattcta
    25981 caatggaatg gatgcgtttt tttgataata gaataaacta tgatatactt atcactaaaa
    26041 gaaagcgcat acttagtgtt ggaggaagga aagtgaaaaa aatggaggag tcatttccta
    26101 aagcagtaaa agtggaaaat atagcgaata ttttgaaagt tacatttgaa aacggagaag
    26161 taaaatacgt gaaatctcat tggacagaag agataaccga tgccttgcaa tttggaaaaa
    26221 aaggaagagg aaaaagaaaa aatcttctcg cactttctcg taatatgtgg attgggactg
    26281 aagtaacgat tgaagcagat ggaacagtat tcataaatgg aaaagacagg tatacgccag
    26341 aagagttgtg gtacaaagga aagaaaagta taccagaatt ataaaaaata attaaaaaaa
    26401 acagcgcgaa acaaggaaca tattacttcc tcgtttcttg ctgttttcta ctatttcagc
    26461 gctcttatga ctcaaatcta aatgtggttg tcacaacctt attctgcacg caaagcgata
    26521 gcggcgtctt ttttcgctgc cattcgtgca ggaagatgac cgccaagcat cgtcaatata
    26581 gtagagacga tcactagaat aatcgcatgg acaggattta gttgagcaac attttttaga
    26641 tccgtcatgt tgtataagat gctattgata gggaacgtag ctagccatgc aataaacaca
    26701 cctaatatcc cagaagaaat acctaaaata cacgtttctg cgtcaaatac gcgagtgatg
    26761 tcttttttac gtgcacctaa agcttttagt acaccgattt cttttgtccg ttcaatcaca
    26821 gatgtataag taatgatact gatcatgatc atacttgtta ctaaagagat ccctgcaaaa
    26881 gcaataagga cataagtgat agcatccatc aatccgccag tcagttcagt catcgttcca
    26941 gctaaatcag tataaatgat tttatcttca tctgatttgc ctttattgta atcatctaga
    27001 taatccaaga tcttttcttt gtcttcaaaa ttgttcggat aaatcatgat acttgaaggc
    27061 aagctgtctc cacctaaata actaagcaat gtttgtttgg cattttcgtc aactttttca
    27121 gtagttaaga catttacacc actgtctctt tgagctttga cgatttctga ttctttgttt
    27181 tcgttgacga tttgagtcgt taattgatcg ctataagcga ttcctggaga taagagattc
    27241 attgtagaag aagatttcac gcgtaggatc cccgagatct tcaattcatc agaagcattc
    27301 tgatacatcg catcataatc tgtattaggg ataaaattcc cagttggaag ttttgtatag
    27361 taagtattgt tgtttactaa tttgaatgtt gtaccaacga tatcatcaaa atcaagtttt
    27421 tcgtcttctt tcacatcaaa tccaagattc ttcaatgcgt tgatattagt attattattg
    27481 ccgtctacga tcagcacgac atctgttgct gaggctggat aacttccggc aagtaaagaa
    27541 taattgtctt ttaaaaagtt ttctttgcta gtgtcaagct gtgtagggaa acttgacact
    27601 cccacacctg tcattgctga catcgtacta gataaagaaa gagattccgt atcaggattt
    27661 tgattcgaga agctgacagg ctgtactttg ccatttacat cacgtaaaag attgattcct
    27721 gtagtacggg taaatccgat attattactt aagttaggat cgatgtctgt tacgtaatcg
    27781 acgtatgttt gatcaatctt attcgtgtgc tgtgctttat cttcttcgct gacttttgca
    27841 gtaatcgttt tgctgtctgg ataatccgct ttgcttgcac ccaatccggc atcgtcacga
    27901 gtctggttag tcgttacctt agaaatagta atcggaaatt tcgccatcgt ttcagcttgc
    27961 gtgttatcga tttgtttttg gaatccagaa gaaagggaca gaacgatcgc gattccgatg
    28021 atcccaatac tggaagcaaa ggaggtcaag aaagtacgtc ctttttttgt tcggatattg
    28081 ttgaaggaaa gctttaacgc tgtccaaaaa ctcatctttg ttctgcgtaa gttaaattga
    28141 tcatcttttg gtcgttcgat gtgaggatgg gaatctgtta agatattacc atcagaaaat
    28201 tcaatgattc gatctgcgta ttgatgggcc agttcaggat tatgtgtgac catgatcact
    28261 aatttttctt tcgataactc ttggatcaat tccatgatct ggatacttgt ttctgtatct
    28321 aacgctccgg ttggttcatc gcacaaaagg atatctgggt cattggctag tgctcgagcg
    28381 atggccactc gctgcatttg tccaccagat aactgatttg gctttttatg catatgattt
    28441 gtcaagccga ctcggtgcaa tgcatcttct gcccgttttc gtttttcgtc tttagaaaca
    28501 ccacttaatg tcatcccaag ttcaacgttt tcaataatgc ctagatgacc aatcaagttg
    28561 taactttgaa aaatgaaacc gatagagttg tttcggtagg catcccagtc actgtcctta
    28621 aagtctttag tagactttcc gttgattacc atatcaccgg aatcataatt atctagtcca
    28681 ccaatcacat tcagcatagt cgttttacca gaaccactag gacctaaaat cgctacgaat
    28741 tctttttgtc tgaaagcgac agacacacca tccaatgctt tggtggtggt ttcgccgact
    28801 ttataatatt tttttatatc ttttaattga agcataatca cccttctccc gtattgaaat
    28861 attcggtata ctaataagta tatcagaaca atgatcgtag tatacgaaat aaatgttaac
    28921 tgaagatgaa ctacaagaaa aaatctagaa atttttcaaa aaacagaggg agatcaatga
    28981 atcgtatttt agtagtagaa gatgatccgc aattacagtt gctttaccgt tctgtgttgg
    29041 aacgagcggg cttttcagtc atcaccgcat tgaatggaga ggatgcgctg aaaaaactag
    29101 aagcgacacg tatagaatta atcattacag atatcatgat gccgaaaatg gatggctatg
    29161 acttgctaga ctcattacgt agcaccagat ttgacacacc tgttctaatg attacggcaa
    29221 aggccgattt tgaggataag aaacgcgggt tcaaattagg agcagatgat tatatgacaa
    29281 aacccatcga tgtcaatgag atgatcctac gtgtggaagc attgcttcga cgttcaaaga
    29341 tcaattacag cagtagcttg ataatcggac aaacaaaatt agagctagat gcttatacag
    29401 tgttacaaga tcagcattcg ttgattctgc ctcaaaaaga atttctgctt ctgtataaat
    29461 tactgtctta tcccaacaag atcttcactc gccaacagtt gatggatgat atttggggat
    29521 tagatacgaa tacagaagaa cgaacgatcg atgtacatat caaacgttta aggacccgtt
    29581 ttgaaaagac ggaagatttt caaatcatca cagtacgggg actaggctat aaggcggtta
    29641 tttaaagatg aaaaaaaagc tattttcaca attatggatc tattttgcta gtattgtctt
    29701 catttcgatc ttagtgacac tgctgtgttt tctcggtttt attttcttat tgtctcgaca
    29761 ttctatctcg cctgctgaat cggctggaaa accaacgtta tacccgttat tcgtctttgc
    29821 ggggttcagt atggtcgtgg gtacaggaat atcgattttt gttgggcggc gtatcctgca
    29881 tccaatcagt gcgctaggga caaatatgag tctggtagca acgggagatt tttcgattcg
    29941 aatggatgaa cagcagaaag tagcagaagt acagcaactg tataaagatt tcaacgttat
    30001 ggtccaagaa ctcaatagta tcgagacact gaggaatgac tttgtgtcca gtgtatccca
    30061 tgaattcaaa acaccactag caacaatcca aggatatgtt caattgttac aagccccaaa
    30121 tctctctgac gaagaacgcc aaatattttt atatcggatt atcgaaagta tcacgcagct
    30181 ttcacaatta acagagaaca tcttaaaatt gaacaaactg gaaaaccagc gtatccaact
    30241 ggaaaagaaa gagtatcgat tagatgaaca aatcagagaa gtaatcgttt tttttcagcc
    30301 taaatgggag aaagaacagc tagaattgga tatcgaactt gctgcagtaa attacacagg
    30361 caatgaagag tttttgtacc aagtgtggct gaatatcatg gataacgcca taaagtacaa
    30421 tcaaatcaat ggacagattc atatcaaact atttgagaca gccacagaga ttgtactgga
    30481 agtgaccgat tcaggtgtag gaatgaatga agagaccaga gatcgaatgt ttgaaaaatt
    30541 ttatcaagga gatacaagtc gtcaaatttc tgggaatggg ctaggacttt cactggttaa
    30601 aaaaattcta gaactgcatg atggacgtat tgattatagc agtatcgaag gagtaggaac
    30661 gacagcaatg atacggttaa gtaaacaata ggaaaaagaa taaaacccct aaaaattcta
    30721 ttgaaggttt ttaggggttt ttagtcgttt ttttgtgatt agggaaatgt tcttacggtt
    30781 cttttggcat aaaaatcgct gctttcttat tgaactacga gaaatgttag aggtgtcatt
    30841 gaaacaagga catgatgcaa gaaagagagc gaaagtcatg cgtcgctctc tttttttggg
    30901 tggatagatg gacagaaaat gtagggctta gttttcttct tgatttaaat actcccaaaa
    30961 tgtatctgaa gccaaagaat gctcaccgac tttacgagta aatgcaagat aactctctgt
    31021 tgttggagaa atagggagaa tcacagtatt ttcttttaat agattcttga ctgttttttc
    31081 cataacaata gcaatcccca tgtcatttgc aatcatattg atgatcaagt cgattcttgc
    31141 tcctttataa gagatttttg gttcaaatcc agcttcgcga cacagatcta gtacaggttg
    31201 atacaagttc gtagtggggc caaggagcaa aaagttctcc tttttcagtt cgcttagatc
    31261 caatttcttt ttactagcta aaggatgatt tttaggtaga actgctacaa aatcgtctgt
    31321 ttcagtaggc aaccactcaa tcgaacttgg tggggcctgg aaacttctag caaagtggat
    31381 cgtgtcacta tgttcattgg cgtcattgta agttaattcg acactttcca cttctttcaa
    31441 ctggactgta aattcaggat ggcgtttcaa aaactcggtg attttggaaa aacctttata
    31501 attgatcatt gtcggtatcg ttaagatatt taatgaaaga ttgttgtctt tcgtataggt
    31561 ttcaagcact tcttgcatag catggtattg ttctacgaca ttttttacat atgggagcaa
    31621 taagcttcca gcttcggtca attcgatttt tctatgggcg cgttcgaata atttgacgtc
    31681 taattctgtt tctaacgaaa gaatctgttt agaaatattt ccttgggtgg tgaattgtcg
    31741 atcagcagtt ttggtgaaat tcattgtttc ggctaaatca agaaaaactt cgaatttatg
    31801 gatatccatc atattcctcc tacttattcc aataaagaat actttaacac aaaaaatgaa
    31861 ttggtgtaat cttctctgtc tgttacaatc tttcttgcag cgaaagcaat aattacagaa
    31921 atttttctga tttttttcgc ttcgtagaaa aagagaggag ttttttgatg ccaactggag
    31981 ttttgatcaa tgtaggttcc gttttgtttg gcggactaat tggcggatta gtaggaaata
    32041 aattaagtga acattttaaa gcacagctga cgatggtttt tggtgtgtgt tcgatgggga
    32101 tgggaatcta ttccattgcg ccgatgaaaa atatgccggc tgttattttt gctttagtga
    32161 taggaacagc gattggtttg attgttcatt taggtaatgg gataaataag ggggctgcgc
    32221 tgatgcaagt acctatttct aaaatcttcc ctagtgaaaa gcttggtatg acccacgatg
    32281 aatttatcag cacattagtg acggtcattg ttctattttg tgcaagtggt acggggatct
    32341 atggaagttt agattctggg atgacaggag acagtacgat tttgatttct aaatctgtcc
    32401 ttgatttttt cacagcagct atttttgcgt gtaatttagg gtatgtcgtt tctgtgatag
    32461 caattcccca attcattatt ttttatattt tgttcttatt ggcaaaattt atttatccat
    32521 tgactacacc agacatgatc ttagatttta aagcttgcgg cggattcttg atggtcgcta
    32581 ccggttttag aatgatcaac gtgaaaatgt tcccagtagc tgacatgata cctgcaatga
    32641 tcgttatcat gccactgagc tggatgtgga caaattggat tatgccactg ttataataaa
    32701 aatcaaagag aagccatttt ttgctgacta aaaaggatca aatcttttag tcagtttttt
    32761 tgtatttgac aaactgattt tgaaactata ttatatgttg tataatatag attgaaataa
    32821 aatattacac ttgagaatag aaggtgacgt aatgcacatc cagattccaa ctgttcttct
    32881 tgatggtact gtgctagcag cattaagcaa ggaagatatg tatgggtatg ccttaacaaa
    32941 aatcgtacaa gaaagtctct ctgtcagtga atctacacta tatccggttt tgagaagatt
    33001 gaaaaaagaa ggatatcttg agacctatga tgaacctttt gaaggcagaa accgtcgata
    33061 ttactcgttg acaccaatgg gaagtcgtca tttagaagaa attattgatg aatggattgt
    33121 ttttcgagat tctttagacc ggttattgga ggagagcaca aatgaatgaa tatttgaatg
    33181 aagttgtgga tcatcttcaa gaaattcctg aagacgatcg ttttgatttg atccagtatt
    33241 atgaggaata ttttttagat tctggaaaaa cattagatca gatcattgat gaatatggta
    33301 caccaaagca gtttgcctta aaattaaaaa tcagttactt ttcagaagaa gaacaagcag
    33361 aaaacgagtt agtgaaaaat ccaaaaagac aaattcgcct gatttggtta atcattatcg
    33421 gtttatttgc ttctcctgtc ctcattccac tagcactggg gtttatattg gttattgggg
    33481 gactattggt tgctttacta gcgctcattt ttagtatcta tgttttatgt atcagtatct
    33541 taggtgttgg cctattcatg atcatctcag gaatcagtgt gtttggacaa tcgattctga
    33601 gtggattatt ctttatcggt gtcggtctgt tcgccacagg aagcgcagta tttatttcgc
    33661 cactgttgct gaaaggaaca aaagggcttt tttatgtcat gatgaacttt gttaaatgga
    33721 tgggacgacg ttttattaca aaaagagatc tgcattcagc gggcgtttga aggaggacac
    33781 ataaattgag attgaaacat tttttgatta ccggtattgg tctgatgctt actggtggcc
    33841 tcctagcagg tgcaacttat acattaggcg ctcaaaagag tctgacttgg gataatggtc
    33901 ctcggttgat cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca
    33961 tccatgcaga aaatcaaaca gtttcggtca tacgcggatt ggattttgag ataaaaacga
    34021 cttatgataa gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg
    34081 cagtcaaaaa atcaacaggt gcaatcatta gtttagaaga cacaagtgca caaatcacta
    34141 ttgttgtccc tgcagatcat gaattatctg aattgacaat cgaagggaat aatagtgcaa
    34201 ttgatatcaa cgaagtatca atcaaaaaag tgaatactaa gacacatgat ggctgggtat
    34261 cgttcaatga atttacagga ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat
    34321 tagatgaggc caatgtggat aacttgatcg tcacaggtga aaacagcaac ttccatctag
    34381 aagacagttt gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc
    34441 atgagatgga aaacagtggc tatactatcc aaacgtctgg tgaatcttat attgaaaaag
    34501 actaccagcc aataacaaat actttgttag aacaaggaca gaaaaagcta aagatcactg
    34561 ctaaagacac atcgatcaat attacgacgg atcaagagga caatgaaaca gattgatcct
    34621 gtactcaact ggatatttta agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt
    34681 catcgcttaa cgcacataac tatagtaaaa taagaaagaa aatatctaaa aagaaaacag
    34741 ggtgaaagag tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga
    34801 aagattacat acttatatca ctcaactaag ccaaaccttc tatgatccag acaaagaaaa
    34861 agtgatggtt aattacccga acaattcaga aggtcgccaa ttagagcaag tgtaccatga
    34921 agttttcaaa catttattga cggtgaagaa agagctggat tattattctt taccgattat
    34981 cgatacagga atattgaagt atgatgggaa gaaggaacga tttatcttta aatctgttcg
    35041 ggaagatttg cctctttcag cagggatgga tctagaggtc ttggtagaag actattttac
    35101 agaagaaaag cactgggttc gaacaaaatt ggattattta ccgcaggcag ccggagggac
    35161 acaggcaagc ggttggtaca tcactgaaga caaagagtta gaactagaag gtgtaatggc
    35221 gcgtattcga aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg ataaaggagc
    35281 gcaggaagta gcatgagaga aaaaagagca ttttatatca atggttatgc agggattgtc
    35341 ggattggtcg tattagcttt agttggattg tttttcttct atttaggtat gtggcaagcg
    35401 aaagtttggg cacttttttt aagtattttt ctttggttga tcacgttatt gttgttaagt
    35461 tcggcaactg tagtcagtcc taatcaagca aaagtaattt tgttttttgg acaatattta
    35521 ggtacgattc gagaaaatgg ctttttcctt acgatacctt tggcacaaaa aatgactgtt
    35581 tcgttgaaag ttcggaattt caatagttct gttttaaaag tcaatgattt ggatggaaac
    35641 cccatcgaaa tctcagcagt agtagtcttt aaagtcgttg atacagcaaa ggcattattc
    35701 gatgtagcgt attatcaaga ttttgtagaa atccaaagtg agacagccat tcggcatatc
    35761 gctagccaat acccgtatga tacattcaat gagaatgatt tgacgcttcg aggaaacacg
    35821 acggcagtgt ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc
    35881 gaagtaatcg aaacacgctt gaaccattta gcctatgcaa cagaaatcgc tagtgccatg
    35941 ctccaacgtc aacaagcaaa agccattttg tctgctcgac aaaccattgt tgaaggagct
    36001 gtcacaatca ctcaaatggc cttagagcaa atcgaagatg gacaagaaat caatttcacc
    36061 gatgatcgga aagtccaatt gatcaataat ttacttgtct caatcattac agacaaagga
    36121 acgcaacctg ttatcaatac aggcgacatg aaagaccgta agtgacatac tttttgtaag
    36181 ataggaagaa ggagtttagg acggattatt gtcttgaact ctttttttag ttgttttaaa
    36241 gaaaatgaca aaattgaaag aagaatgata gttgattcga aggcagaatt gttcgctttt
    36301 ttctataatc agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg
    36361 tctacttgtt ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt acgtagttat
    36421 gggtggagaa gaatactatg tacaaattac gacagatggt acaaaaacag tttcaaaatc
    36481 agactctgga caagaaacag tcaactatag atatatcctg cctggctata cgaaagaggg
    36541 taaggaaaaa gaactggact tcaagggaca attgactcga ccgcttcgta aaggagcata
    36601 tttaaaaatc acctggaaca aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa
    36661 agacgtgccg aaaacagcac tggaaaaact aattaaaggg tagggaaaga aatgaacaaa
    36721 atagtcgaag tacagaatgt aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa
    36781 gcattaagcg gcatcagctt cgatgtagaa aaaggcgaat ttattgggat catgggcgct
    36841 tctggttctg gtaagtcgac acttttgaat attttatcca cattagataa accaacagat
    36901 gggcatatcc gaatcaatca aaaagatgtg accacattaa aaggaaatca actagcagat
    36961 tttcgtgcga atgagattgg ctttattttc caagatttca atttattaga aagcttgaca
    37021 gcacaagaaa atatcgctgt tcctctttct ttacaaggcg tcagaccaaa agaaatcaag
    37081 caacgtgtcc aaaaaattgc agaacgttta tccatctctc atattttgga aagctatcct
    37141 tctgagattt ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa
    37201 ccaacgattc tattagggga tgagcctaca ggtgcgctgg attccaaaag tgcccgggat
    37261 cttttagata cgatggacga attgaataca aaagatcatg tatcgattct tcttgtgacg
    37321 catgatccgt tttctgcaag ttattgccaa cgtattttat tcatcaagga tggcggtatc
    37381 catcaagaag tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga aatactatct
    37441 attcttggaa acttggaaca gtagggggag attgactatg ctttggaaat tatcacttac
    37501 tgggatcaag agccgtttaa gagactactt ggtgttattc tcaggattag ttatggcatc
    37561 tgctattttt tacatgtttg aatcaatggc aagcaacgaa gcttttttga aaagtaatac
    37621 gatcgttgcg gctacagtta tcattttccg attcggttct gttttactag ggatcatcac
    37681 attcgtttat attttatatg caaattcctt tttgatgacg atgcggcaaa aagactatgc
    37741 aatgtttatg atgctgggag caaaaggcag aaaaatcgca cagatgattt tttctgaaac
    37801 attcttagta gggatcgcag ctactttagt gggttcagct gtcggtgtag gattgacaag
    37861 tatcgtccac cgattattag tggatcagtt agatctcaat atttcgcatt ttacaccttt
    37921 tagtattcac gggttgttga tcactgtttt gttttttgct gttttgtttt tattagctgc
    37981 tgttgtcaat gctttttcga ttgtcaaaaa accgatttta gcattgattc gtgcggatca
    38041 gacaccaaca agaatcaagc agcataaatt cttgtttttc cttgaagtag tactgggaat
    38101 tgcttttcta ggctgcggtt actatatgat gaaacacgtg gggacctatc aagtctttgg
    38161 tataggggta gccttagtta cgatcgtatt aggaacttat ttcattttcc attctgtgat
    38221 cattttcttt ttatcattac taaagaaatc agaacagatt tcattgaaaa aattgaacaa
    38281 tttcacattg tcacagttaa actttcgtat tcgtgaatat acacaaatgc tctcaatggt
    38341 cgctatgctg tttgccttgg cactgggcgc tttgacggta ggattaggtt ttaaaaatga
    38401 aattgcagac ttgacagaaa acaattctgg atatgatcta gtgatgaata atcctcgggc
    38461 agaagatcag caaaaaatca aagaactttc accaacactt tctgcttctt atactcaaaa
    38521 agaagatgcg ggaacgattt actataatgc aagtgaattt gcggctcaac cattgatcat
    38581 gataaaacat gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa
    38641 aatgaatgaa ctgactgtac aagaggaact tcgaagctat gaattgccgg aacagcaaga
    38701 aaaagaaatc aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc cacaatccac
    38761 tttacagcta gttcaggtaa aagacttcca agcaaacctg aaaccaatcg aaacacttgt
    38821 tgctcaaaat aaaatgaaca atccatcttt gcaaaaagta gagtacagca accagaaata
    38881 tgaaatgtat gaagtgtata atggtctatt ctctggtttt gaatttatgg gattcttcct
    38941 aggtttagct ttcttgacca tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag
    39001 caagagtgac attgctcgtt atgaaatgct tgaaaagata ggaacacgac gcgggttgct
    39061 taaacaatcg atccgtagag aagtaggcgt gttattctta gcaccaggaa tcttaggtgt
    39121 agtccatgtc ttattcggat tacaaatgtt tggtctattc atgcaaaatc catacaaaga
    39181 tatttgggtt ccgtttacga tcttcttcgt attgtatttc atctactatg tactgacgac
    39241 atggttatat actggaatcg tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag
    39301 taataagaac agccaagcaa aaatagctcc tcatattttt cgtttggttg ggcttattac
    39361 cgcagatgca agcttttgaa caccgtttgt tcggaaaaag aggttgtgac aatatttttg
    39421 tcacaacctt tttttcacgg tcttttatta gaatatagta aattttttct cgttactcac
    39481 ggaataagct gttaagaaaa cggtttccat gtatactgga atcagtgcaa atgaaagaaa
    39541 agagggtgca aggaacatgg aaatcaatga acaaaatttt ggcgaaaact atcacttgat
    39601 tacgatcacg aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg cacgggttgt
    39661 tcattttcaa acaataatcg ataatgaaaa acgagagttg atcttaggtt ttgatgaagc
    39721 aaaagaatat ctagaaaaag acccttatat cggtgcttca atcggacgta tggctggacg
    39781 gattacgaat ggcacattca aattagatga tgaaacgttc gaagtaaaaa cagacccgga
    39841 cactggtcat tgtttacatg gtggtgcacc tagctttgaa gccaagcagt ggcattatac
    39901 agtcatcaat ggtgaaaacg aagcatcggt cattttctat cagactagcc cagatatgga
    39961 aaacggattt cctggaactt tagatgtaga agtacggtat acgctgacga acgatgatac
    40021 atggcgagtg acggtccaag ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca
    40081 tgtctatttc aatttaaccg gtgatccagc acagtcaatc gacgaacata tactttggtt
    40141 gaatagccga ttatttgcta cgctcaataa agatacaacg cctacaggag aagtagtttc
    40201 agtaagtggc acagcatttg acttccaacg tcctaaaaaa ttaggtgagg tctttgccag
    40261 tgattttcgc caatcaatga tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca
    40321 atctggttta ctaaatgctg cggaattgac aagcccagat gaaaaagtat cgatccaagt
    40381 aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat tttggagaag atggaccgga
    40441 aatgcgcggg aataaattag taaatcatgg cggtatcaca tttgagactc aagaactgcc
    40501 aggagcggag cgacttgaaa gttttggaga tgtcactctg gaacctaacc aagtacgcga
    40561 gtttgtaacg gaatacaaaa taaatataaa gaaatagaat aaattaaaaa gggagtgtga
    40621 cagtgtcaca tccccttttt aatttattct atttctttaa aggcagatca agccagaatg
    40681 atttgtctaa acttaacaag gttagtacag gaacagttgc agctttaact gtacgagtag
    40741 ttgtttctgg tatctgtctt gccgtaaaat tgttccacaa atcccagatt gaagcgacac
    40801 ggaagatgga agaagcgttg ttgacgcgtt ttatcgttga aaattctaat cctgtgattg
    40861 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    40921 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    40981 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    41041 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    41101 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    41161 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    41221 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    41281 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    41341 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    41401 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    41461 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    41521 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    41581 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    41641 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    41701 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    41761 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    41821 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    41881 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    41941 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    42001 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    42061 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    42121 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    42181 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    42241 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    42301 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    42361 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    42421 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    42481 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    42541 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    42601 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    42661 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    42721 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    42781 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    42841 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    42901 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    42961 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    43021 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    43081 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    43141 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    43201 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    43261 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    43321 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    43381 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    43441 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    43501 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    43561 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    43621 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    43681 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    43741 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    43801 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    43861 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    43921 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    43981 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    44041 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    44101 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    44161 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    44221 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    44281 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    44341 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    44401 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    44461 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    44521 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    44581 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    44641 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    44701 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    44761 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    44821 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    44881 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    44941 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    45001 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    45061 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    45121 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    45181 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    45241 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    45301 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    45361 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    45421 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    45481 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    45541 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    45601 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    45661 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    45721 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    45781 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    45841 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    45901 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    45961 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    46021 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    46081 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    46141 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    46201 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    46261 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    46321 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    46381 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    46441 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    46501 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    46561 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    46621 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    46681 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    46741 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    46801 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    46861 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    46921 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    46981 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    47041 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    47101 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    47161 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    47221 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    47281 aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca tattatctgt
    47341 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    47401 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    47461 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    47521 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    47581 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    47641 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    47701 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    47761 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    47821 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    47881 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    47941 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    48001 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    48061 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    48121 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    48181 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    48241 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    48301 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    48361 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    48421 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    48481 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    48541 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    48601 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    48661 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    48721 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    48781 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    48841 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    48901 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    48961 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    49021 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    49081 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    49141 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    49201 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    49261 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    49321 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    49381 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    49441 ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    49501 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    49561 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    49621 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    49681 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    49741 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    49801 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    49861 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    49921 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    49981 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    50041 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    50101 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    50161 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    50221 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    50281 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    50341 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    50401 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    50461 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    50521 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    50581 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    50641 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    50701 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    50761 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    50821 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    50881 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    50941 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    51001 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    51061 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    51121 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    51181 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    51241 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    51301 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    51361 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    51421 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    51481 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    51541 taaattttac aaacgctttc ggttcagtta taataataaa tgataacttg tatatataag
    51601 ttgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    51661 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    51721 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    51781 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    51841 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    51901 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    51961 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    52021 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    52081 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    52141 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    52201 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    52261 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    52321 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    52381 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    52441 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    52501 attttgcaga attaaagcga ctatttcata catggggaga aaagatgagt gagggtaacg
    52561 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    52621 ctatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    52681 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    52741 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    52801 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    52861 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    52921 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    52981 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    53041 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    53101 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    53161 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    53221 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    53281 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    53341 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    53401 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    53461 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    53521 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    53581 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    53641 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    53701 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    53761 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    53821 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    53881 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    53941 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    54001 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    54061 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    54121 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    54181 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    54241 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa catttgatga
    54301 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    54361 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    54421 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    54481 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    54541 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    54601 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    54661 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    54721 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    54781 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    54841 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    54901 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    54961 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    55021 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    55081 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    55141 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    55201 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    55261 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    55321 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    55381 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    55441 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    55501 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    55561 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    55621 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    55681 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    55741 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    55801 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    55861 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    55921 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    55981 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    56041 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    56101 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    56161 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    56221 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    56281 cactgatagg caaaaaagaa ccgaagggtt tggatcggct ggtcatttag tcggtccaaa
    56341 ctattcggtt ctttttatga aatcaatttc tttaatccac ggatagaggc tgggatggtc
    56401 tcgccgtttg ccaacgtgac ttctctggtt tttttattaa tcgaccgcac attttgtgta
    56461 ttgataacat aggatttatg acaacggata aaattccgat gcattttctc gatttctttg
    56521 atcttctcat agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct
    56581 cctagcgaag tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta
    56641 aactctattt tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg
    56701 ctcaggatat gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca
    56761 gtcggttcga tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag
    56821 attatttttc ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt
    56881 tcactgtctt ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg
    56941 gctgcattga gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt
    57001 tcaatcatga ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct
    57061 tcacaaatat aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt
    57121 tgagaaaaac gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc
    57181 aattcatcca agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac
    57241 cctttttgtt tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc
    57301 atcacggaat gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta
    57361 gctccttcaa tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt
    57421 ttcgtatcca gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt
    57481 gccatcatga gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac
    57541 atacttagat tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca
    57601 taataagacc gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc
    57661 agattttggt aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg
    57721 atataatcct gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta
    57781 gagattttcc aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa
    57841 ctcgtattca aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg
    57901 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
    57961 tgaaaccaag tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg
    58021 gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
    58081 acaaaaaaat cgtcgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
    58141 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
    58201 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
    58261 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
    58321 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
    58381 tacagctccc attgattttt accaaattat agcacaatta gcgtattatt tttttaattt
    58441 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
    58501 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
    58561 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
    58621 tagaaaagta agaagctgag aaaaataaag taatgagaga aaaaattaga aacatgctga
    58681 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
    58741 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
    58801 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
    58861 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
    58921 agatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
    58981 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaaggca aattttactt
    59041 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
    59101 tcacaacttt atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat
    59161 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta
    59221 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
    59281 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta
    59341 taaaaggtga tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat
    59401 atgcatttaa atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg
    59461 aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata
    59521 atctgcactg gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa
    59581 aatgttgaga gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa
    59641 tgttaaaaaa cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg
    59701 agaatactgt ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc
    59761 atgaatagga gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt
    59821 caaggctttt atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag
    59881 gatgccgata attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac
    59941 tgaaatgctt gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga
    60001 agtattgccg ctattttatt ctaatccaga aaagtatact tttttattac agattttctt
    60061 tttgaacaaa cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga
    60121 ccgctccatc tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc
    60181 tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga
    60241 aatttcgcca caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat
    60301 gttagcgaga atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact
    60361 ctattcctac tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat
    60421 atgtcccaaa atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg
    60481 agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc
    60541 ccagtcgatc cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata
    60601 atataaaaac taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt
    60661 agtttttttg attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa
    60721 atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg
    60781 ggcgccaaac agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac
    60841 gacacttaga tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc
    60901 atataagttc gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg
    60961 cccgttgatt ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt
    61021 tactaatgcg ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc
    61081 ataatataga acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag
    61141 atactttgtt agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac
    61201 acttagccat tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc
    61261 agaggcaaaa gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt
    61321 cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt
    61381 ttatgaatat ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt
    61441 cagaaaacta aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa
    61501 gaaaaaatag acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa
    61561 agatgggaag gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt
    61621 gaaacgctgg gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat
    61681 cattgctacc tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca
    61741 agtaaaagaa agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa
    61801 tcgcttcaat caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat
    61861 catagcagga gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat
    61921 gacgcaaaaa caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc
    61981 aaatgaacta aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg
    62041 aacattccat agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt
    62101 agcaatgcaa ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt
    62161 tttgcaacag ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac
    62221 gattgcttct cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca
    62281 gttagttaaa gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg
    62341 actgtatgga ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt
    62401 caatgatgta ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat
    62461 cggccaaaaa tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg
    62521 ggaaatcgtg aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga
    62581 tgatcagagt atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa
    62641 agaatatcct accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt
    62701 ccaaggagca aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat
    62761 tccagcacaa aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga
    62821 aagtcagtgg ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga
    62881 atcaatcata ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa
    62941 aacgaatatt ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa
    63001 gtctttccaa catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa
    63061 gaaaaatcag gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa
    63121 aagtgaatgc gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat
    63181 tttgtcgata agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc
    63241 gttgcgtaaa acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct
    63301 gccacgtata gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa
    63361 gcagcatgct actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa
    63421 agaattaaaa acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca
    63481 gagcattcac aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga
    63541 aggaagtttg ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa
    63601 agcagaacca gtgacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag
    63661 agcaagaaat cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt
    63721 atcgagattt atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg
    63781 gtgtgacaga agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa
    63841 atttttgtga aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg
    63901 aa
//
LOCUS       DACCIQ010000007        58687 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCIQ010000007 DACCIQ010000000
VERSION     DACCIQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 58687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.007"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(58..186)
                     /locus_tag="IVM62_000441"
     CDS             complement(58..186)
                     /locus_tag="IVM62_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6844434.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="IVM62_000442"
     CDS             complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="IVM62_000442"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6844435.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1673..2728)
                     /locus_tag="IVM62_000443"
     CDS             complement(1673..2728)
                     /locus_tag="IVM62_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6844436.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="IVM62_000444"
     CDS             complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="IVM62_000444"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP6844437.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="IVM62_000445"
     CDS             complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="IVM62_000445"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP6844438.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="IVM62_000446"
     CDS             complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="IVM62_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP6844439.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="IVM62_000447"
     CDS             complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="IVM62_000447"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP6844440.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="IVM62_000448"
     CDS             complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="IVM62_000448"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP6844441.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10490..12049
                     /locus_tag="IVM62_000449"
     CDS             10490..12049
                     /locus_tag="IVM62_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP6844442.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="IVM62_000450"
     CDS             complement(12160..14091)
                     /locus_tag="IVM62_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6844443.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="IVM62_000451"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="IVM62_000451"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6844444.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="IVM62_000452"
     CDS             complement(15020..15772)
                     /locus_tag="IVM62_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6844445.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15931..16140)
                     /locus_tag="IVM62_000453"
     CDS             complement(15931..16140)
                     /locus_tag="IVM62_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844446.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16151..17479)
                     /locus_tag="IVM62_000454"
     CDS             complement(16151..17479)
                     /locus_tag="IVM62_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844447.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17844..18431)
                     /locus_tag="IVM62_000455"
     CDS             complement(17844..18431)
                     /locus_tag="IVM62_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP6844448.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="IVM62_000456"
     CDS             complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="IVM62_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP6844449.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20011..21300)
                     /locus_tag="IVM62_000457"
     CDS             complement(20011..21300)
                     /locus_tag="IVM62_000457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP6844450.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21454..22386)
                     /locus_tag="IVM62_000458"
     CDS             complement(21454..22386)
                     /locus_tag="IVM62_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844451.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22543..23202)
                     /locus_tag="IVM62_000459"
     CDS             complement(22543..23202)
                     /locus_tag="IVM62_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6844452.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="IVM62_000460"
     CDS             complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="IVM62_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6844453.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24243..24533)
                     /locus_tag="IVM62_000461"
     CDS             complement(24243..24533)
                     /locus_tag="IVM62_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844454.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="IVM62_000462"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="IVM62_000462"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP6844455.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="IVM62_000463"
     CDS             complement(26820..28175)
                     /locus_tag="IVM62_000463"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP6844456.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="IVM62_000464"
     CDS             complement(28199..29368)
                     /locus_tag="IVM62_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844457.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="IVM62_000465"
     CDS             complement(29365..30252)
                     /locus_tag="IVM62_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP6844458.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="IVM62_000466"
     CDS             complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="IVM62_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP6844459.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34319..34840
                     /locus_tag="IVM62_000467"
     CDS             34319..34840
                     /locus_tag="IVM62_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6844460.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35539..36104)
                     /locus_tag="IVM62_000468"
                     /pseudo
     CDS             complement(35539..36104)
                     /locus_tag="IVM62_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36283..36939)
                     /locus_tag="IVM62_000469"
     CDS             complement(36283..36939)
                     /locus_tag="IVM62_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844461.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="IVM62_000470"
     CDS             complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="IVM62_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP6844462.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38476..39474)
                     /locus_tag="IVM62_000471"
     CDS             complement(38476..39474)
                     /locus_tag="IVM62_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP6844463.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="IVM62_000472"
     CDS             complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="IVM62_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP6844464.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40490..41221)
                     /locus_tag="IVM62_000473"
     CDS             complement(40490..41221)
                     /locus_tag="IVM62_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP6844465.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41225..42490)
                     /locus_tag="IVM62_000474"
     CDS             complement(41225..42490)
                     /locus_tag="IVM62_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP6844466.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="IVM62_000475"
     CDS             complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="IVM62_000475"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP6844467.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="IVM62_000476"
     CDS             complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="IVM62_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP6844468.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47832..49334
                     /locus_tag="IVM62_000477"
     CDS             47832..49334
                     /locus_tag="IVM62_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6844469.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49336..50073
                     /locus_tag="IVM62_000478"
     CDS             49336..50073
                     /locus_tag="IVM62_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6844470.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50118..50279)
                     /locus_tag="IVM62_000479"
     CDS             complement(50118..50279)
                     /locus_tag="IVM62_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6844471.1"
                     /translation="MIIIVSEGGIDYVLDIVNEPMVLSYAMEQVERLLVCAGEQAMRL
                     VQLSEKTRN"
     gene            complement(50441..51607)
                     /gene="dnaJ"
                     /locus_tag="IVM62_000480"
     CDS             complement(50441..51607)
                     /gene="dnaJ"
                     /locus_tag="IVM62_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP6844472.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51758..53587)
                     /gene="dnaK"
                     /locus_tag="IVM62_000481"
     CDS             complement(51758..53587)
                     /gene="dnaK"
                     /locus_tag="IVM62_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP6844473.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53638..54201)
                     /gene="grpE"
                     /locus_tag="IVM62_000482"
     CDS             complement(53638..54201)
                     /gene="grpE"
                     /locus_tag="IVM62_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP6844474.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54295..55338)
                     /gene="hrcA"
                     /locus_tag="IVM62_000483"
     CDS             complement(54295..55338)
                     /gene="hrcA"
                     /locus_tag="IVM62_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP6844475.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55573..56739)
                     /locus_tag="IVM62_000484"
     CDS             complement(55573..56739)
                     /locus_tag="IVM62_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP6844476.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56755..56982)
                     /locus_tag="IVM62_000485"
     CDS             complement(56755..56982)
                     /locus_tag="IVM62_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP6844477.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57369..57625)
                     /locus_tag="IVM62_000486"
                     /pseudo
     CDS             complement(57369..57625)
                     /locus_tag="IVM62_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57968..58433)
                     /locus_tag="IVM62_000487"
                     /pseudo
     CDS             complement(57968..58433)
                     /locus_tag="IVM62_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16152 a        12734 c        10236 g        19565 t
ORIGIN      
        1 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
       61 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
      121 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
      181 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
      241 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
      301 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
      361 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
      421 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
      481 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
      541 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
      601 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
      661 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
      721 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
      781 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
      841 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
      901 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
      961 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
     1021 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
     1081 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
     1141 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
     1201 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
     1261 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
     1321 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
     1381 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
     1441 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
     1501 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
     1561 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
     1621 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
     1681 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
     1741 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
     1801 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
     1861 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
     1921 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
     1981 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
     2041 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
     2101 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
     2161 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
     2221 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
     2281 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
     2341 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
     2401 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
     2461 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
     2521 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
     2581 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
     2641 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
     2701 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
     2761 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
     2821 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
     2881 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
     2941 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
     3001 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
     3061 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
     3121 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
     3181 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
     3241 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
     3301 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
     3361 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     3421 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     3481 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     3541 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     3601 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     3661 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     3721 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     3781 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     3841 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     3901 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     3961 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     4021 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     4081 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     4141 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     4201 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     4261 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     4321 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     4381 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     4441 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     4501 ttgtcccgct tttttgaagt aagcgtcata ataaccagca cttaaggaga atgtacctaa
     4561 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     4621 atctaatgat ttcacatttt ctgagcgata accgtagcgg ataccatcga atcgttgcaa
     4681 gttggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     4741 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     4801 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     4861 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     4921 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     4981 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     5041 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     5101 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     5161 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     5221 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     5281 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     5341 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     5401 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     5461 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cttcgacctc
     5521 aggctctgtt tctttgattc gttcaaatgt tgcttttgtc aaatcttgta cagtgatttc
     5581 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     5641 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     5701 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     5761 acgtttactg tttcaaccac attcgacgta aatggaacac cttctgtatc tacttcttcc
     5821 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     5881 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     5941 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     6001 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     6061 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     6121 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     6181 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     6241 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     6301 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     6361 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     6421 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     6481 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     6541 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     6601 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     6661 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     6721 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     6781 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     6841 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     6901 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     6961 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     7021 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     7081 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     7141 aacttgtaag ctgctgccca tctcggtgct ttgacagtaa aaccaagttg atcttgtagc
     7201 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     7261 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     7321 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     7381 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     7441 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     7501 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     7561 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     7621 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     7681 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     7741 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     7801 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     7861 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     7921 tattgttcct caattgctac taattctgcg tattctttat cataaacata gtcttccact
     7981 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     8041 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     8101 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     8161 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     8221 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     8281 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     8341 gaatacaggt gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     8401 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     8461 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     8521 gcgttcttct tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     8581 ttctagacca atcaggaata caactgggaa ttctaatcct ttggctgcat gcaaagtcat
     8641 caatgtcact tgggaagcat cttcttctaa attgtcaata tcggaaacaa gtgccagatc
     8701 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     8761 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     8821 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     8881 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     8941 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     9001 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     9061 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     9121 ttgaggattt gccagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     9181 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     9241 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     9301 gcttcgaatc tcttcttgca tccgatctac gataaaacgg gtttcgtctc gttcattatc
     9361 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     9421 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     9481 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     9541 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     9601 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     9661 ttggtactca tctacatgaa tataatggaa tttgttttga taatatgtga gtgaatcagg
     9721 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     9781 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     9841 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     9901 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     9961 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    10021 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    10081 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    10141 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    10201 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    10261 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    10321 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    10381 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    10441 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    10501 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    10561 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    10621 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    10681 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    10741 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    10801 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    10861 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    10921 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    10981 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    11041 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    11101 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    11161 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    11221 tatttatcag aaggtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    11281 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    11341 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    11401 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggtgg
    11461 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    11521 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    11581 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    11641 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    11701 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    11761 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    11821 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    11881 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    11941 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    12001 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    12061 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    12121 agaagagact gggaaaaacc agcctcttct tttttatttc tatgcttctg ctgctgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccat gaggagccag
    12301 taatttgatc ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcagctct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 cagagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccggcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagcaatt ttttaccata cggaatgatt tcttctacag attggaaggt tgtattgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaatg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagccggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ccaaaccgtt aatttcagtt tcagtatctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg gtccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattaaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctacgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactcg caatataaat cagtcaaaaa
    15841 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaaa aagactggtt
    15901 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    15961 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    16021 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    16081 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    16141 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    16201 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    16261 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    16321 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    16381 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    16441 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag cgattctcgt
    16501 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    16561 ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    16621 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    16681 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    16741 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    16801 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    16861 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    16921 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    16981 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    17041 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    17101 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    17161 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    17221 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    17281 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    17341 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    17401 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    17461 atttatcttc gccaatcatt ttctcacctc tttaaaatct ttttataatt taattataac
    17521 caacgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    17581 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    17641 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    17701 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    17761 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    17821 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    17881 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    17941 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    18001 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    18061 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    18121 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    18181 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    18241 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    18301 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    18361 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    18421 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    18481 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    18541 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    18601 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    18661 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    18721 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    18781 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    18841 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    18901 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    18961 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    19021 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    19081 tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt tcagcaaggc
    19141 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    19201 aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca cattgtaatc
    19261 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    19321 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    19381 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    19441 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    19501 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    19561 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    19621 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    19681 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    19741 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    19801 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    19861 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    19921 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    19981 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    20041 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    20101 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    20161 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    20221 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    20281 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    20341 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    20401 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    20461 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    20521 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    20581 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    20641 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    20701 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    20761 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    20821 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    20881 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    20941 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    21001 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    21061 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    21121 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    21181 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    21241 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    21301 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    21361 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    21421 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    21481 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    21541 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    21601 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttcattta acacggaact
    21661 aagcagcgca tcttcattac cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    21721 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    21781 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    21841 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    21901 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    21961 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    22021 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    22081 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    22141 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    22201 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    22261 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    22321 tgctagtgct tgttgtgcta ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    22381 cttcacgcag tgtcctcctc ttgctcatgt tcagctcatg ttcaatagta gcaaaacaaa
    22441 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    22501 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    22561 gttgaatgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcctag
    22621 atgttgcttt ttctgattaa agattgctgg aagataccct aacatcagca aaggaaattt
    22681 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    22741 atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttgac catgattgaa
    22801 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagatact
    22861 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    22921 cgtaatagct ccgatacaca aaagatcgac caagaaagca aacattcgga taaaaaaacc
    22981 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    23041 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    23101 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    23161 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    23221 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    23281 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    23341 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    23401 tttcgcatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    23461 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    23521 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    23581 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    23641 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    23701 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    23761 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    23821 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    23881 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    23941 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    24001 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    24061 ctgtctccct cttccaacac atttgaagct agttcttcat ccccatataa ccaactgttc
    24121 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    24181 acaaataatc ctgcagcaat cagaactgct gcccaacgtc tcttattcat tctttctcct
    24241 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    24301 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgtcaattc atcttctacc
    24361 gtaaactcac gagcttcagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    24421 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    24481 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    24541 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    24601 cccattattt tttgacataa aaagagcatt tgacaaagca tttgatatat accaatttat
    24661 tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc
    24721 agataaaaaa caaaagagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tatctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgctg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaatg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagattctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctctggaaaa attgacacag atccctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagatagccg ccaagttctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gcgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gattggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttactcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccatcatt gatatttaac
    27541 ccgtctggat ttgtgcccat ggtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacctc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgattg
    29221 gcaccccagt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatca gttctctacg
    29461 caaaattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaatgtaat
    29521 gctgacatcc ccagtttctt cagagacaac gatggtaatc gcgtcgctga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttcttttgga atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctct ccagtgatat ctgcatctaa
    29761 tgcaattcct gtctcaatat actcatctaa tccagtatgc cgttcgatag taattagcgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat ctgctcgtat ttttcttgag aaaaacgcat aattttcagg
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttctt tcttctgctt caaggtccgt cgttttttct
    30541 tttctagtct taacgggtac atcgatagat tcctctggtt caagtcgttt cttgattttt
    30601 tctaattctc ccgtcagtgc agcatagttg taaaaacgtg ttttagcgtc ttggatagtc
    30661 ttttcaaata aagcttttgc aagatttgga tttgcgttct ctagcgagga aaagcgaact
    30721 tgtttcctca tgaaatcagg catagcagag aaatcaggtt ttttgtagtc caaagtcatt
    30781 gggttcttgt ttttttcgac cattgcaggg ttatatcgat ataatgacca atagccagaa
    30841 gaaacagctt cttttgcttc tttaagcgat tctttcattc caccaagcaa accatgtgtg
    30901 atacaaggcg tataggcaat gattaaagaa ggtccaggat acttttctgc ttcttcaaaa
    30961 gcctttatcg tctgcatttg attggcacct gaagcaattt gcgccacata aacatttcca
    31021 tatgtcatcg ccatcattcc tagatctttc tttgaagcat actttccact agccgaaaat
    31081 ttagcgattg ccgaagctgg tgttgctttt gaggtttgac cgccagtatt ggaatagact
    31141 tcgttatcca aaacaagaat attcacgtct gctccgctgg caagtacatg atcgatcccg
    31201 ccaaaaccaa tgtcgtaagc ccatccgtct ccaccaatca tccattggct gttttttacg
    31261 aatagatcct ttctcttgta tatatcctcc agttcaggga caattgatcg ttcgctttcg
    31321 atcgcagctt gcagttttgc tgctctttgc tgcgttcctt cgctctcaaa catgtgtacc
    31381 aaccagtctt ccatcaattc tcgtagttct tctgatactt ttggcaatac ttctttgatt
    31441 gaacttgcca aagcctcgcg acgagtctgt gccgctagaa gcataccata accaaattca
    31501 gcattatctt ccaataaaga atttgaccat gcaggccctt gaccacattc attggtcgta
    31561 taaggagaag caggagaagc accgccccaa atagaagaac agcctgtcgc attcgcgatc
    31621 atcattcgat caccgaacat ttgtgtcaat aattttacat aaggtgtttc gccacagcct
    31681 gagcaagcgc ccgagaattc aagaagcggt tgattgaatt gtgtagacaa gacagtattc
    31741 tttcctttga ctggatttgc tttttgtctt aatgtcattg caaaagccca attcacagct
    31801 tgttctttct gttcttcata aggcttcatc actaaagctt tttcttttgc gggacaagcc
    31861 tctacacaca gaccacaacc tgtacagtct tccactgaaa cttggatacg gtattttacg
    31921 ccgtctgcgc ctcgcatttc tctcacgata aatccttctg gcgcttcttc catttcctct
    31981 tcatctgcta aaaatggacg gatggcagca tgaggacaaa caaaggcaca ttcattacac
    32041 atcgtacagc gatcactaat ccactcaggt acttctaaag cgacgccccg cttttccata
    32101 gaagccgttc ctaaaggcat ttcgccagtt gtcattccat ttgctacaag tgtaccaaca
    32161 gagagttgat ttccttcctg tgcattcact ggttcaagta tttcttgtat ataaccagtg
    32221 ttccttttat tgattttttc aggcatctcc aacgttctcc aatggtctgg gatcgcgact
    32281 tgatgaagca gttcggtcgt ccgatcgatt gcttgtatgt ttttttctac gatagccatt
    32341 gatttatgtc catagctttt cagggcttct tcttttagca gcggaagcac ctcatcaaaa
    32401 ggaatgatgt cagctaattt aaaaaaagcc gtttccattg ccgtattgat ccggcgtccc
    32461 aaaccgactt cttgggccaa tctcatggca ttgatcgtat agaatctaat gttattttca
    32521 gccaagtaac gttttaattt taagggcaga tgcgtttcta attgttcgtc gctccaaaga
    32581 gtattcaata aaaaaatgcc acctggcttc aatcctttga ccaagtcata gctatgcagg
    32641 taagctggtg tgtgacaggc aacaaggtcc gcatgctcga caagatacgc tgaacgtatc
    32701 ggcgtatctc caaaacgaag atgagagaca gtcagaccgc cagatttttt cgaatcataa
    32761 taaaaatagc cttgcgcata tttatcggtg tgatctccaa taatcttgat agctgattta
    32821 tttgctccaa ctgttccgtc agatccaaat ccccaaaact tggcctggaa tgtttgcggt
    32881 tctgtcaaat ccagactttc ctttggttcc aaagattgat aagtcacatc atccacgatc
    32941 cctatggtaa accgtttttt gcgtatgctt gggtcttttt tcaattcatc aaaaactgct
    33001 gagatttggt cgggagtcac atctttagac cccaatccgt agcgaccacc aataacagcc
    33061 gggcgaagtt cagaatcata taacgcactt tgaacgtcta ataagagagg ttctccacct
    33121 gcccctggtt ctttacttcg atcaagaaca gcaattgttt ttactgtatg aggcaacttt
    33181 tgcacgaatg tttccactgg aaaaggacga taaagatgga tattcaaaaa acctgttttc
    33241 cgtccgtttg cattcaagta atcaatcgtt tgttcaatcg tctgtgcaac tgatcccata
    33301 gaaacaatga cttcttccgc atcgtctgct ccgtaataag tcaccaagtc ataagaagtt
    33361 cctcgtaatc ggttgatctc tttcatatat ttttgaacga tttcaggtaa tttatcataa
    33421 taggggttga tcgtttcccg ttgttgaaaa tggatgtctg gattctggtt cgtaccacta
    33481 acagtcggat gattgggatt catccctcgt ttacgaaatg aagctaattt ctcctgattg
    33541 atcagaggag ctaattcttc atattccaaa acttcgattt tttgaatctc gtgactcgta
    33601 cggaatccat caaagaaatt caaaaaaggt acttctcctt caatagaagc aagatgggca
    33661 acaggtgcta aatccatcac ttcttgtaca ctgctttcag ctaacatagc aaatcctgtt
    33721 tgtcttgcag ccatcacgtc tccgtgatct ccgaaaatat tcaacgcatt tgtcgtcact
    33781 gcccgactag caacatggaa aacacttggc agtagttccc cagcaatctt gtacatattc
    33841 ggtatcatca aaagcagccc ttgagaagcg gtatatgtag ttgtcagtgc accagttttc
    33901 aatgatccat ggaccacacc agctgctcct gcttcggatt gcatttctac tacttttact
    33961 ggttgaccga atacattttc ttttcctgct gctgaccatt gatctactaa ttcagccatg
    34021 gtcgaactgg gtgttattgg atatattgca gcaatctcgg taaatgcata agaaatatat
    34081 gcggctgccg tattgccatc cattgttttc atttttcgca tgattcatca tccttgttca
    34141 tattctctct tattatctgt tccattataa cgtaaaacat tccatttttt cttttattta
    34201 taaaaatatt tcacatatgt ctatttgaat tggtcatcta atttttagac aaacagtcta
    34261 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    34321 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    34381 atcgaatgct cctaaaaaaa tggtcgaatt catcaaccta tcttgtccgt attgccgaca
    34441 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    34501 agtgatcaaa ttgttcgata aagaaaaaga aagcttgcag cgaggaaacg tgatgcaccg
    34561 gtatctcaca atcagcgatg gtcaaaaagc gatcaaagag atcaaacaaa tattcgatac
    34621 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    34681 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    34741 agcacatatt cgatttgttc cgactgtcat cttgggtaaa gaaatctttg atgaatctat
    34801 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtaggac
    34861 aaaagtaaag aacacttttg tctcacgctc taaacaccta taaacggcgg aagcagaagc
    34921 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    34981 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctgagcaaat
    35041 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    35101 tttcatacaa aatttctcaa attcccttaa cgagtaatat ttatgtacgg tgattcacac
    35161 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    35221 tttatgtatg ctgttagttg attagatggt cgtttgttga gctaggtcgt ttattttaaa
    35281 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    35341 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaactc ctaactcctc
    35401 tcatttaata gttgctctct tttttattca gatacatttt tgctgatcac agcctagtat
    35461 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    35521 tggattaata gatttcgatt attcaaagcg attttctatt ggtggattgt gttcccaact
    35581 aacaagacgg attccaaagt cattatataa ctcttcaaga gttaaccctt tcctgttccc
    35641 aatagatgta tctctcccac gctcgatata aaatatggag tttcgaatgc caagacctgg
    35701 caaatctttc cttgttacag ttacactgct actaacttcc ccatataaaa tccagctcct
    35761 ccaaaatgat aatatatttt tcgaccatca tctaactcat gtaacaataa ttgttgtgcc
    35821 ctcttgtcac tcgccaaatg caaacgccac gtgcgatacc atgccatgct acctgcatct
    35881 gcagaaaatc catcaaataa tgcaacaatt gtatcttcta tcacaatcct ttccccatct
    35941 acttcatatt ctaatctaaa atcaaactcc cctcgggtaa tcgcaggtct tggtggctta
    36001 ggacccaata ttattctact taaaaccgca ttcgttccca gatgaaacac agctataaca
    36061 acaataatga ttaatatttt gtttctattt gataaatttc tcattattcg caaatcacct
    36121 ctacattctc acatactagt tcgtaatact cctttaatat caaaatatct actgatcgta
    36181 ataatattcg ctatgaattc ctactactcg tataagaaag gccaaatcat cagcttttac
    36241 ttgctgatga tttggcttgt ttctatttat cgtaattgtt ttctaatcac caaaattgtt
    36301 aacaatcggt tcagatattt cccaattcac tagccttata ttatattgat ttaacaattc
    36361 ttcttcattt attgtatttt gatcaagcgg accaaattgt tctaataatt ctttgtcatg
    36421 taaaatatcc cttctagtaa tgcctctttc cctttcaatg aaagaagcac taggaaaagt
    36481 atgattataa ccattcaacc tatctcccat gtaataatta gcaggtcctg gaaaataaaa
    36541 tatttgctta ttatcactaa cttctaacag caacactaaa tcttctccgc tggctaaacg
    36601 acttgtccat ctacgatatc tccctatgcc ttcgttcatt ccaaatccat caaattcaac
    36661 tattaacgtg tcttctatca catgtaattc ttcattcaat tcatactcaa gtctaaaagg
    36721 aaattctcca tatgtaatct ctggcaatgg tggtgctgga gttctccaaa taaatacaat
    36781 aactgtgaat agaaaaaatc caacgatcga tataaatgtt atgagacctg ctcgaagtaa
    36841 aacccgatat aataaaaatg ctgtatttcg ctttgctata tctagaaaaa taaaaagtac
    36901 tgcaatcata atgatagtaa aagttgtaaa aaaacgcatt tttgccctcc ctttataaac
    36961 atcgattctc aaattctaaa ccaaatcttg gtagttcaag ttcaggtata acgtattcca
    37021 accatacatc tcaattacta tttatgaact tatgattatc actgaataaa tcactccatc
    37081 cccttgttaa aacttctaca ttaattacac ctggttgttt tcgactagct aatccaatga
    37141 acttatcaac catccttatc caccaaccac gagaatcttg accagaagta agaagagcag
    37201 ttctcaagga tgatcgagca gttccttcca gttttttaat taatcaggcc agttatagat
    37261 aaatacttga tcttatctat atcaggactt ctatattaag caaaaattta tgtacaaaac
    37321 taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa acaatagaag
    37381 cctttccaaa ttatgttact ccagtaaaga tggaattata gtttgacgga agaaaagcaa
    37441 gtataacttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat cagttttttc
    37501 tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt tgcgaattcg
    37561 gtgattaatt cccgattttg atatcgcacc tgagggaatc atttccccta attctttcaa
    37621 gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt caggaagggc
    37681 atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag aagccgcatc
    37741 aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg aatttctcat
    37801 atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac caatcaatgt
    37861 taagaaatca gcaatttttt ctgctccttt taaataagaa atatagccat tgcgccgttc
    37921 aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt ggttatgctg
    37981 ttcataaata gaaaatattt ccaaatgata acggctcgtt tccggattat tgactgaacc
    38041 tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc ccataatctc
    38101 gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca tcaacacatc
    38161 ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt tcattttttt
    38221 acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat agatacgtct
    38281 ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca caatacctaa
    38341 agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc gatgaacttc
    38401 caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct cctcctttct
    38461 ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt cttctactac
    38521 tttgtctcca tcgtggaaga caccaccatc tttcaattcc aaaaagtcgg tggaaatcac
    38581 tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta gatactcatc
    38641 atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca atactgtatc
    38701 gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat ctgtgaaatg
    38761 ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag cagttgtctt
    38821 gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg tgaataaact
    38881 tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt ttcgagccgc
    38941 tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg ttttacggtc
    39001 gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaagacag catgcaaggt
    39061 caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt ttgctaataa
    39121 ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga tcagattacc
    39181 gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga aaatatcttc
    39241 atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac caggaggagc
    39301 catagcgatc gattctctga ttgcaccgct gcttccccca tcatctgcaa cagtcactac
    39361 tgctgtgata tctgctcctt gatttcgcaa acttttcagg ataactggca gaccagtgcc
    39421 accgccgacc actacgatct ttggttttct tatccggtag gttttcattt tcacgaccgg
    39481 ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt atagtttttc
    39541 cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc gcctgtacag
    39601 ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa tacagtttcc
    39661 agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat cacataatca
    39721 tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg agggttgggc
    39781 aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt aaaaccaaat
    39841 gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc attgattttt
    39901 tcacgtaatt gtcgtggtgt caatgtcgtc gtatcaatga tcacggaagc ttgggctttc
    39961 aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc gtccatcgcc
    40021 attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc tgaagcatct
    40081 aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag catgttttgg
    40141 atttcttcaa agaaggaacg agaacgtaaa tccactccta aagccatttt tgtgactttg
    40201 cctgattctt tgattaattc ccagaatttt ggaatcaatc gaggcggcat attatcgata
    40261 caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc accgctcata
    40321 cctgttatga tgactaattg caaattatct gtcataatta cgaactcctc tcacattgtg
    40381 catagtaaaa ttatatcatt ccgggcgtat cattaccagt tttctttttc ttttacaaaa
    40441 agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt tacttttccg
    40501 atgctgtatc caatgccttg cttctttcag ctagcgctcg ttcaatcgtc cgatctgcta
    40561 aaatagattg ggcatcgatc actggataat ggttatcttc agcaaattca tgcatcaatg
    40621 acaattctgt gcaacctaag ataacttttt cacagtttag gcgctctacc gcttcttcta
    40681 agatttcgta atatagctct tgattcaaat aatccgattc cttgatctca tgatagatca
    40741 gataattgat tttttcttga agaacagtat caggaatcac tgtttcgaat ccaagatttt
    40801 tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga atcgctactc
    40861 tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata tgaaggatag
    40921 gaatatccgt tgccgcttgt aattcttcaa aaaagtagtg tgcggtgtta caagtcaaca
    40981 caatgaagtt cggctgcaat aaattttgtt tctcaatatc atccagcaga aacggcattg
    41041 gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt gcatgattga
    41101 ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg ttgatcaagc
    41161 gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa aaattctcca
    41221 tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag ttatggaaca
    41281 tatatttcat caacaaccag cgcttgattg accggtcttt ttcataaaac acagtagtac
    41341 cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt tcccgagcat
    41401 attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct gattgattcg
    41461 ttccataggt cgtttcaaca aagggtttgt taaacactcg atcttctgtg acgaaacgag
    41521 ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc aaattgattt
    41581 caaataattt atattctcca tctctaggat catacttcat atcgaaattg gcaaagcctg
    41641 tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg ttataatctg
    41701 gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaac agtggatgac
    41761 caagacacat catccgaact tgatgatctt catctacata cgcatttagc acacgcatat
    41821 tactatcatc acccgggata aaatcttgta caatcatttc acttttataa ccggcttcgt
    41881 atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa gctttttttc
    41941 ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa gcaactggga
    42001 attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct ctgacgatca
    42061 atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt actttattga
    42121 tcagtctttc aaataaagaa taatcaatat atggacagat aaaatattct gacagctctt
    42181 gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa agatattttt
    42241 tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata aacacagggt
    42301 ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg gtaggggcta
    42361 actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa gaacgtgcca
    42421 taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca ttttcaatac
    42481 tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca actacacaag
    42541 gaatatcata ccataaagaa aaaagcaatc aaaggaaact cctccgattg ctcaattctt
    42601 ttttgaacgg attgaactta taggaagttt ttgtctatac caatcagtgt agaaagaact
    42661 accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc atttttttat
    42721 gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt caaatagtga
    42781 cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc aaggatcgtt
    42841 cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt tttgattaca
    42901 tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct ttgtaataca
    42961 tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc tagtatatag
    43021 aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg ttgtgcctca
    43081 ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc aacatcaaca
    43141 atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac agcatcttct
    43201 actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc taatgtttca
    43261 gagttatatc gtttgccatg acatacttca caaggaacat agacatcagg caaaaagtgc
    43321 atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc ttttacgttg
    43381 aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg agcaaataac
    43441 ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg cgtccggccg
    43501 attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga aattgtcttg
    43561 aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt ctttaagata
    43621 ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac aaattctcct
    43681 aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt gatcttgatt
    43741 gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt ccctgaaaga
    43801 tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc tgcagccacg
    43861 atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcagataatc tgctgcccac
    43921 atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg cattttcttt
    43981 aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg ttcatctaaa
    44041 atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat tcgctgcgct
    44101 tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag tcccacattt
    44161 tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat tactttctct
    44221 tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga caactcattt
    44281 gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt caatcgataa
    44341 cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc acgcgtgaaa
    44401 tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc gaaagtcgtt
    44461 tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag atcatctgtc
    44521 ccattcaaga tgatatcctg atgctcttcc ggtaattctt cgaatggtgt atctaaatcg
    44581 ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga gctgattgga
    44641 ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat cactaaatct
    44701 tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc aaaaggcgca
    44761 ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata aggacaggca
    44821 tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc atagccgtct
    44881 gccaatcgca gagccgcctc taaagaatca aatagacgag aacgaatccc ttctttcaca
    44941 atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag ctcaggcact
    45001 tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc tttttggatt
    45061 cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc caacacttgg
    45121 atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac tgattgactg
    45181 gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa caacaagcgt
    45241 aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt acttgttgtt
    45301 ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc tatctacgtc cggcttatcc
    45361 atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct ccgctgccct
    45421 tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa acctgtaacg
    45481 accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg ggcacgtgca
    45541 ccgtgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc ttttaattct
    45601 aaaatcatat ctcgcacatt agcggctgtt tcaaaatcca atgctttcgc cgcatttttc
    45661 atttcttttt ctaatttcat caataacgta tctttttctt ctttcgtcat ttcttcatac
    45721 gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga aatcaaatct
    45781 cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta cttctgctga
    45841 atcgtacggc gtcttgatgt ttcatccatc gctagacgca tagaatctgt tactttgtct
    45901 gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt ttggactaag
    45961 gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac aagggaaact
    46021 tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc aaattcacct
    46081 aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact atgcaaatat
    46141 ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat ttttttcgtc
    46201 aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc gcctactaga
    46261 tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag ccctgtcgga
    46321 cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc tggcgtcgca
    46381 gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag tggccgattg
    46441 tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct cgctcggtca
    46501 ccattataca ttcctctgac ttgaggcatg gttacgtgcg attcgtccgc cacgatcaaa
    46561 aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt acgtccgtcc
    46621 atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat catttcaata
    46681 tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc atttcgcaag
    46741 acagttaaac gctgttctaa ttccgctttg atattcgcta cggcatgctc catatgttca
    46801 tcattcgtca caaagtgagt cgccgggaag attgccacgt gttccgtttc tcctaaaact
    46861 tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa aaactccaca
    46921 cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc gcgcactcga
    46981 aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac taagtcacga
    47041 atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg tttttggtac
    47101 tcaaaaggcg aacctaatcc aaaaatacat gaaacggatg caatgacgat cacatcattt
    47161 ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc atttacactt
    47221 gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg ataataatca
    47281 taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa ttccccgtac
    47341 agctgaccag ccaatgtttt gttatgggca ataattagtg ttggtttatt cactttctca
    47401 ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa gatttgtgct
    47461 tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg atcaccagcc
    47521 ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat cattcttcgt
    47581 cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg aaagcaaagg
    47641 cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt ttatcttatt
    47701 atgcatgtta cgttttataa cataaaatgt tagaaactcg catttttttc attttttcga
    47761 gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat tggaaattta
    47821 ggaggttgat tatgaaaaga aagcatttat ttcactcatt tgttttctta atgacaggac
    47881 ttgtgacact tgcgtttgga catgttgcac aagctgacaa cactacaacg gcagagtcaa
    47941 gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga cgtatacgta attgccagtg
    48001 actctgcttt tgctccattt gaatttcaaa acgcaaaggg cgagtacgaa ggcatcgatg
    48061 taaaattgat ggaaaacatc gccaaactgc aaggatttaa catccgaatg gactaccgtg
    48121 gtttctctgc tgctttgcaa gctttggaat caggacaagc agacggaatg attgccggaa
    48181 tgactgtaac agatgaacga aagaaaagct atgatttttc tgattcttac tttgatagcg
    48241 ggatccaatt agctgtcaaa aaaggagata cttcgatcaa gtcttatgaa gatttgaaag
    48301 gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga atttttaagc gaccatgaat
    48361 ctgaatatgg ctattctatc aaatattttg atgctgctga tcaattgtac gacgggttaa
    48421 aagttggcgc aatcgatgca atgatggatg attatccggt aatcggttat gcgatcaaac
    48481 aaggacaaga attggaaaca ccgatcaaaa aagaatctgg cggaaaatat gcatttgccg
    48541 ttaaaaaagg acaaaatcct gagcttctta atatgttcaa tgaaggattg aaagaattaa
    48601 aaagaacagg cgaatacgat aagattatta aagaatatgt agcggatggt tcagaaactt
    48661 ccagtgagtc ttccactgct gatgaatcaa catttcttgg acttatccaa aacaactatc
    48721 aagcattgtt gaaaggactt tggagaacaa ttctcctaac tttgatctcc tttgctttgg
    48781 ctatagtaat cggtgtgatt tttggattat tcagtgtagc accaatacgc gctcttcgca
    48841 caattgctag tatttatgtc gatatcatcc gaggaattcc aatgatggtt cttgcgttct
    48901 ttattttctt tgggttgcct ggtatcgttg gatttacgat tcctgacttc cttgctggtg
    48961 ttatcacctt gactcttaac gcaagtgctt atatcgcaga gattgttcga ggcgggatca
    49021 acgccgttcc tgtcggacaa atggaagctt ccagaagttt aggtctttct tataatcgga
    49081 caatgcaaaa aatcattttg ccgcaagcga tacgcatcat gatcccatca ttcgtcaatc
    49141 agttcgttat ctcattaaaa gacacaacga tcatttccgc aatcggtgta gtcgaattgc
    49201 tgcaaactgg taagatcatt gttgcacgta caacacaaag tacgtatgtt tacttgatca
    49261 ttgcgatcat gtacttgatt ttgatcacag cattgacaaa attagcaaaa gttttagaaa
    49321 agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc atttagtcaa aaaatacggc
    49381 gataacacag tattaaacga catcaatgtg tccatcaacg aaggagatgt cgtctgtatc
    49441 attggccctt caggttcagg aaaaagtaca tttttgcgtt gcttgaatcg tttagaagaa
    49501 ccttctgacg gtgaaattat tattgacggc gcccacttga tggataaaag cacagatatc
    49561 aacttggtga gacaacatat cggaatggta ttccagcact ttaacctatt ccctcatctt
    49621 tctattttgg aaaacatcat tttagcaccg atggacctga agaaagaatc tagagaagaa
    49681 gcagagaaaa aagcaatcaa attgctgcag acagttggtc tagaagataa aaaagatatg
    49741 tatcctgaaa tgctttccgg tggacaaaag cagcgtgtgg ctattgctcg tgctctagcg
    49801 atgaaccctg acatcatgct ttttgatgag cctacatctg cactcgaccc cgaaatggtc
    49861 ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag gtatgacgat ggttatcgtt
    49921 acgcacgaaa tgggcttcgc taaagaagtg gccaatcgtg tcatgttcat tgacgacgga
    49981 aactttttag aagacggaac gcctcaacaa atctttgaaa atccgcaaaa tgaacggaca
    50041 aaagatttct tgaataaagt attaaatatt taataaaaac caaaaatgcc cggtcgcttc
    50101 atgacgaccg ggcattttta gtttcttgtt ttttcactta actgtaccag ccgcatcgct
    50161 tgttctcctg cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc
    50221 ggttcattga cgatatcaag tacatagtcg ataccacctt cagaaactat gatgattatc
    50281 ttattctaaa gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga
    50341 acgtatgatt gttcgtctgg tttttaatta ttaatgactt agcgttttta ctagaaatag
    50401 aaaatttggg caagtctact ttatattttt actagaaaaa ctacttccgc tttttcccgc
    50461 caaaagcatc tttaaactta tcgaagaaac cgtcttcttg ctgttcttca actttgatgc
    50521 cgccagcttc agcgaatgca cgtaatgctt ctctttgttg ttcatttaga tttttcggtg
    50581 tgatcagttt tactttgaca tgttggtcgc ctgttgcacc accacgtaac ttcggagcgc
    50641 cttttccacg taaacggaag ttcgtacctg tctgtgttcc agcagggatc ttcaatttca
    50701 catctccatg gacagttggt acttggactt catcccccaa agctgcttgt acaaaactta
    50761 aaggcaattc atagtaaatt tcagaacctt cacgatcaaa gatatcactt tcttctacac
    50821 gaaaaaccac atacaaatca ccaaatggtc cgccgttttc tccagcttct ccttgaccag
    50881 ctaaacgcat ttgttgtcct tcttctacac cagctggtac attcacttta actgaatgtg
    50941 ctttcttctc atgtcctgag ccatgacagg ttggacaagg ttctttgatt tctttccctg
    51001 tgccatgaca aacatcacaa gtttgacggc tcatcactcg tccaagtggc gtttggcgtt
    51061 ctacattgat cgtacctgca ccatgacatt tatgacaagt agttggttgt gtgcctggtt
    51121 ttgcaccgtt tccaccacaa gtatgacaga tttcttcccg attatacttg atttcttttt
    51181 ctacaccaaa aatcgcttct tcaaaagaca agtcgaccgt atattgcaaa tcagcacctt
    51241 gacgaggcgc atttggatcg actgaccgac cgcctcctcc aaagaaagag tcgaagatat
    51301 cttcaaaacc accgaagccg ccaccagaga agccgccgaa tccaccaaat ccgccaccag
    51361 ctccaccagc accgtaattt ggatctgtgc ctgcatgccc gtattggtcg tatgcagcgc
    51421 gtttttgcgg gtcacttaaa atctcatatg cttctgatac ttctttgaat ttttcttccg
    51481 catcaggttc tttattgatg tcaggatggt attttttcga aagcttacga taagctttct
    51541 ttatttcatc ttctgatgct ccttttgaga gccctaagac ttcataataa tcacgttttg
    51601 ttgccatagg ctttcctcct attggttctc atcattttca cgtcgcttgt tatcatatca
    51661 tattttgttt gaaacaaaaa ccgatacttc aaactatttt tcaaaagaaa aagccaaagt
    51721 agcaactttg gccttctctc gaaattcagt gaactgatta tttatcgtcg ccatcgactt
    51781 cttcaaaatc agcatcaacg acatcatctg ctccgccttg agctgcttct ggattttctt
    51841 gagcttgttg ttgtgcagct tgttcgtata gtttaacagt caagttttga acgatttcgt
    51901 tcaatgcatc gcgtttagtt ttcatttcct cgatgttgtt tgcttcaaca gcggctttta
    51961 attcgtcacg agctgtttct gcttttttca cttcttcttc gtccactttg ccttctaatt
    52021 ctttcaatgt tttgtccaca gagaatagca atgcgtctac atcattacgt aggtcaactt
    52081 cctctttacg agctttatct gcttcagcgt ttgcttctgc atctttcacc atacgttcga
    52141 tttcttcgtc tgttagacct gaagaagatt tgatcgtgat tttttgttct ttttgtgtac
    52201 ctagatcttt cgcagataca tttacaatcc cgtttttatc aatatcgaat gttacttcga
    52261 tttgtggaat accacgagga gctgctggaa tatctgtcaa ttggaatctt cctagagttt
    52321 tgttgtcagc agccattggg cgttcacctt gtaatacatg gatgtctact gctggttgat
    52381 tatctgccgc agttgagaat acttgtgatt tacttgttgg aatcgttgta ttgcggtcaa
    52441 tcaatttagt gaacactcca cccattgttt cgatacctaa tgacaatggt gtaacgtcaa
    52501 gtaaaacaac atctttcaca tcaccagtga tcacgccacc ttggatagca gcacccatag
    52561 caactacttc atcagggtta actgatttgt ttggttcttt tcctgtttct ttacgtactg
    52621 cttctacaac tgcagggata cgagtagatc caccgactaa aataacttcg tcgatttctg
    52681 attgtgacag acctgcatct tttaacgctt gacgtactgg tacttttgta cgttctacta
    52741 agtcagctgt taattcatca aattttgcac gtgtcaatgt catttccaaa tgcaatggtc
    52801 cagattctcc tgctgtgata aatggcaagc tgatttgtgt gcttgatacg ccagataaat
    52861 cttttttcgc tttttcagca gcgtctttca agcgttgtaa agccattttg tcttgagaca
    52921 agtcaatgct gttttctttt ttgaattctg ctaccatgta gtcgatgatt ttgttatcaa
    52981 agtcgtcccc acctagatgg ttgtcgccag ctgttgacaa tacatcaaag acaccgtctc
    53041 ctagttcaag gatagatacg tcaaaagtac cgccaccaag gtcgaatact aagatttttt
    53101 cgtcgcgatc tgttttatct aaaccatagg caagagcagc tgcagttggt tcattgacga
    53161 tacgttctac ttctagacca gcgatcttac cagcatcttt tgttgcttga cgttgtgcat
    53221 cattaaagta agctggcaca gtgataactg ctttttcgac tttttcacct aaatagtctt
    53281 ctgcgaatcc tttgatatat tgaaggatca ttgctgagat ttcttgtggt gtatatgatt
    53341 taccttctac atctactttg taacctgctt caccgatatg gcgtttgatt gatgaaatcg
    53401 tatttgggtt tgtcacagct tgacgttttg caacttcccc tacttggatt tctccatttt
    53461 tgaatgatac aacagaaggt gttgtacggt ttccttctgg gttagcgatg atttttgctt
    53521 cgtttccttc caatactgct actgcagagt tagtcgtacc taagtcaata ccgataattt
    53581 tactcataat taacgtctcc ttctttaatt gaaagtgtaa gttttatatc cgcacaatta
    53641 ttgtgcaaca atgaccatag ttggtcttaa cacgcgatca tgtaatttgt agccttcttg
    53701 tagtacttct acaatcgtat cggctggtgt atcttcagtc gctggaacag tttgtactgc
    53761 ttgatgaaga ttaggatcaa aggcttctcc tttagcaggg attttttcaa ttccttcttc
    53821 ttccagtgcg ttacgcaaac tttccaagac catttccacg ccttttttca aacctgcccc
    53881 ttgatcatct gacacttctg tggctaatgc acgttccagg ttgtcaattg atggaagtaa
    53941 ttttttggct aaatcttgtg aacggtattt ttgtaattgc tcacgctcat tttttcctcg
    54001 atttgccata tttgcgattt cagcacgagc ccgcaagtat ttatcttcca tttcttctaa
    54061 ctcagctttt aatttcgctg tttcgaattc ttccgcttca gcttctgaaa tacccgaatc
    54121 ttctgcagct tcgacatcga tttccggttc tggctgagca tctgtcattt ctttatctag
    54181 ttcttcttga ttttctttca cgatacagtc ttcctttcat tattcagtca gctgtttgat
    54241 tgctcttact aagaaagaga gaacgatcag ctgcaaatag cttcgttatt cacattacga
    54301 gtttgacaag tcaagcgagc ggtagtaatc agcaagttta gctgctagtt ctcttcgata
    54361 aacatcgact agaccgaaca tccgagaata aggcatacta gtaggcccta acaaggctat
    54421 tgtacctctt ccatgaccat ctatctcgta acttgcttga atcatactca tattctgcaa
    54481 taaatcattg cctaattcag caccaatctt gatatgaata ttgctatcca tcggagtgat
    54541 catttgtgta agtccgtctg agtctttcat aaaagaaaac atcgatttga actgatcaag
    54601 atcttgacgg ggctcataat ccaagagatt catttgtcca ctgacataaa ccttttcttc
    54661 aaaaacatga ccaagaacgg tgtcaaataa ctgagacatc ccttctgtcg tctggaaata
    54721 tttatgaaga atcatcggaa tctccgtacg aagtttatgg tacaccgtca tcagaggatc
    54781 tccgactaga cggtcgttta caatctgtac cattttttct agttcctgac ttcccatata
    54841 ttcgggaaga gtgaacactt ggctttctac attccccata tctgttacga cgatagcaat
    54901 aatctgtcga ttattcaagg gtacgatacg aaagccagtc agtcggcggt cttttatttc
    54961 aggtcccaat gaaaaagtcg tatagcttgt cagctcagat aatatctcag cagattgctt
    55021 aataatctca ttgatttcat gaaattctct gcctagagac tgacgaatca gcagcaaatc
    55081 atcttctgct acgcgagcag gttttaacaa atgatccacg taataacgat agcctaaagc
    55141 agatggaatc cgcccagaag aagagtgagt cttcaacagc aatccttcat cttctaatgc
    55201 tttcatatca ttgcgaatcg ttgctgagct ggccttgact ccctcagaca ttaatgtttt
    55261 ggaaccaacg ggcacgccac tactcgtata gtgttggatg atcagacgta aaatgtcgtt
    55321 ttgtcgcttt gttaacatct tcctcacctc tcattagcac tcatatattc taagtgctaa
    55381 ttcacttata aatatagcaa gaagcccttc tcctgtcaag agaaagactc cattttttag
    55441 cactctaaac acaagagtgc taattctaaa taattgatgt aaatccagtt atatcaatac
    55501 ttcaatacct cattgtcaaa ctttctaaac tttttctttt caagtttcga taaaaaacaa
    55561 atattcttca aactattttt caaataaaaa gcgctcaaac acattgttcc ccaaaaaagt
    55621 ccctcttggt gtcaaataaa cacggtcttc ttcattaaca agtaaccctt cttgctctaa
    55681 ttcccttagc acatcaccgt acaccgattc aattgttttt tgatagcgat catagaaatg
    55741 ttttttagta atccctgttt tttttcttag tcctaagaac atttcttctt ctatttgatt
    55801 ttttcgtgtc aatttttctt cttccaatac cgggcgttca tttgctcgta atgggcgtaa
    55861 atagtgttgg attggtcctt tgttgcggta acgaatatct ccaagataac cgctagcacc
    55921 agctccaaac ccaaaatagt ggtcattgtt ccagtacatg aggttatgtt gagattcatg
    55981 tccggagagc ccaaagttac ttatttcata ttggtgacgt ccatgctttt ccattgcttc
    56041 aatcgtctct tcaaacattc tggtttccgt ttcctgatca ggcagttcga gtcttccttg
    56101 tcgtacccag ttcatgaaca ttgttttgtt ttcgaggatc aatgagtaca aagaatagtg
    56161 aggcagatcc aaagccaaag ctttttccaa agtatcccga tatccttcta gtgtttgtcc
    56221 cggtaatgca taaatcaaat cgatgcttac attggtaaaa ttttctgctt ctaaaaattg
    56281 cattgtttga tacacatctt ctgcagtatg tttacgtcca atttttttca ataagcgatt
    56341 gtcaaatgtc tgcactccca tggataaacg attgacgcca tagtttttca tcacttgaag
    56401 tttttcacgg gtcaaatcac caggattcgc ttctacagta aactcattac cattttgaaa
    56461 aggcaggatc tctctagcac ctgataatag tcgatcaagt tgtttagcag ataaagaagt
    56521 tggtgttccg cctcctacat aaagtgtttc caaattcgct gtaggatgac tttccagcat
    56581 taatctcatt tcattcagca gcatttccac atactcatct actggctgcc cttctaaaaa
    56641 aactttatta aagtcacagt aatagcagat atgctcgcaa aatgggatat gaatgtacgc
    56701 agaaaccttg ttttctgcgg gatttattgt ttctatcatt gaaattcttt ctccttacgt
    56761 cgaaaagtcg tgcctgtttt cgaccttctt aaaatcgcgt tcaagcacca actcattgaa
    56821 ataagccgca tatttagaat caaggaaacc tagctctaaa aatgcttgct tattgtcatc
    56881 ggagctacac ggtgctttta cagcctctcg ataaacggta atcgcaaagc tgaccttcgg
    56941 cattaagtca aaatctgcaa aaatatgaaa agctattttc acagattcct ccttaagtgc
    57001 tcagaccaca acgctttgct tacaacctct catatacgca tactaaacgt atttggcgaa
    57061 caaatccact aaaaaatcca agaatcgact tcttggatga tttttttaca ctcatgattc
    57121 ttcctaaaca attttgtcct tattttattc ataaaaaaat ctagtcaaat ctctcacttc
    57181 aagagtttta actagattcc aattattatt tttttgtaaa aataaacagt ctcttaatca
    57241 tagacatttc ttcggtgccc tacttgaata gcgataacaa cgaattgatt atcttctatc
    57301 tctaccaata cacgtatttg ccaatcagag ctgcttctga atttcgggcg tgaattttaa
    57361 acggcaggtc attccagccc accaaattca ttaaggattc ttccatgaat actgttgttt
    57421 tcccacttct tatccaatgt ttatgggcaa tttcagccgt ttgacgattg tattcatctt
    57481 ctagttgttc catcgaatag ttttttagaa gttcagataa agtaatccca taaaattcag
    57541 ccatataaga aagccattct tttttggcgt cacttacacg aaccgtaatc atagccctat
    57601 aattgcctct cttaaagaat ctcatgcatt tcctgatgtg attttagaaa aatttacttg
    57661 ctaaaaaact gtgaaaatgt aggagctagc atccttgtag taaatgatcg agctctttat
    57721 tatacattaa aaaagttcag aaattttgat gaagtagcca actacttttt catgagtaaa
    57781 gcaacactag aaaaagaatc ctccagtact tagaatttac tggaggatgt acttttgaag
    57841 aagcctatag gttatttaaa aaatattact acaggaagga acgagaatta cttcgttact
    57901 tataatagaa cttagtctta tatagaaggc atagctcaca ttagcttatt tgtcctataa
    57961 aaataaatta cggtatttta gaaaagctat tttctctttt ttcaacgatc ttcagcaatt
    58021 tttctttatc gtcaggtaac tcaagttcat gaattctagc atgacaattg ggacataacg
    58081 cgatacagtt ttcaatcaca tcttttccac cttgcgacaa atattctata tgatgacaat
    58141 gtaaataagg tacaccgtct tttataaatg gccctttttc ttggcaaagt tgacaaatac
    58201 cttttgcaag tcgtttaaca tattcagata cgaagggatt acgtttatat atttttgttt
    58261 ttgagaagca aaactcctct tcgttaacat attttgcagc cattttttta ttataattac
    58321 taacttttct tgcttcttcc tctaatttag cattagataa attacgaata tgtctagttt
    58381 gattttcttc ctggttatat atctttatct tcaatcaatt gctcttgatt tattaaagct
    58441 aatggaaatt tatatacttt tctgctgttg cccgaactat ctttttcatt aacagtatat
    58501 atttgtcctg ctaaatatac ttctcccaga tatgtgtgtt cttttggttt aaatgtttca
    58561 aataaatgaa tctttacaaa attacttttt gaattggcta aaactttatt ttgattctta
    58621 tccaatactt gatccccttt tagccccata ccggtatatt gtaaaatacc atttaaccac
    58681 ttatctt
//
LOCUS       DACCIQ010000008        53296 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCIQ010000008 DACCIQ010000000
VERSION     DACCIQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 53296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.008"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            68..232
                     /locus_tag="IVM62_000488"
                     /pseudo
     CDS             68..232
                     /locus_tag="IVM62_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            480..698
                     /locus_tag="IVM62_000489"
                     /pseudo
     CDS             480..698
                     /locus_tag="IVM62_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(827..2035)
                     /locus_tag="IVM62_000490"
     CDS             complement(827..2035)
                     /locus_tag="IVM62_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HAP6844478.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2325..3290
                     /locus_tag="IVM62_000491"
     CDS             2325..3290
                     /locus_tag="IVM62_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6844479.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3312..3524
                     /locus_tag="IVM62_000492"
     CDS             3312..3524
                     /locus_tag="IVM62_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6844480.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3599..4075
                     /locus_tag="IVM62_000493"
     CDS             3599..4075
                     /locus_tag="IVM62_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP6844481.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4072..4572
                     /locus_tag="IVM62_000494"
     CDS             4072..4572
                     /locus_tag="IVM62_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAP6844482.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5132..6949
                     /locus_tag="IVM62_000495"
     CDS             5132..6949
                     /locus_tag="IVM62_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6844483.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7058..8383
                     /locus_tag="IVM62_000496"
     CDS             7058..8383
                     /locus_tag="IVM62_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6844484.1"
                     /translation="MQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTIN
                     FSKSMKYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIW
                     YEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLL
                     SAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFN
                     FILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREI
                     SASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTS
                     ENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYK
                     YLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8460..8621
                     /locus_tag="IVM62_000497"
     CDS             8460..8621
                     /locus_tag="IVM62_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844485.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8662..9042
                     /locus_tag="IVM62_000498"
     CDS             8662..9042
                     /locus_tag="IVM62_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAP6844486.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9057..9338
                     /locus_tag="IVM62_000499"
     CDS             9057..9338
                     /locus_tag="IVM62_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844487.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9597..10379
                     /locus_tag="IVM62_000500"
     CDS             9597..10379
                     /locus_tag="IVM62_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP6844488.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10552..12270
                     /locus_tag="IVM62_000501"
     CDS             10552..12270
                     /locus_tag="IVM62_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP6844489.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12272..13126
                     /locus_tag="IVM62_000502"
     CDS             12272..13126
                     /locus_tag="IVM62_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844490.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13802..14122
                     /locus_tag="IVM62_000503"
     CDS             13802..14122
                     /locus_tag="IVM62_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844491.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14146..14556
                     /locus_tag="IVM62_000504"
     CDS             14146..14556
                     /locus_tag="IVM62_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6844492.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14703..14909
                     /locus_tag="IVM62_000505"
     CDS             14703..14909
                     /locus_tag="IVM62_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6844493.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15256..15777
                     /locus_tag="IVM62_000506"
     CDS             15256..15777
                     /locus_tag="IVM62_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844494.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15835..16260
                     /locus_tag="IVM62_000507"
     CDS             15835..16260
                     /locus_tag="IVM62_000507"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6844495.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16355..16669
                     /locus_tag="IVM62_000508"
     CDS             16355..16669
                     /locus_tag="IVM62_000508"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAP6844496.1"
                     /translation="MPNGYKIVRQSLVDDLEGEKKVCWETEILQDDDSGFTYEISRCL
                     YFDTCKEHGYAEFCKVFCNHDWYAYGVLKHHSRFIRKSTIAEDGTVCHDTIEKVTEKH
                     FL"
     gene            16703..16885
                     /locus_tag="IVM62_000509"
     CDS             16703..16885
                     /locus_tag="IVM62_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HAP6844497.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            16998..18509
                     /locus_tag="IVM62_000510"
     CDS             16998..18509
                     /locus_tag="IVM62_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844498.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18832..19056
                     /locus_tag="IVM62_000511"
     CDS             18832..19056
                     /locus_tag="IVM62_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAP6844499.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19049..19615
                     /locus_tag="IVM62_000512"
     CDS             19049..19615
                     /locus_tag="IVM62_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAP6844500.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19603..19833
                     /locus_tag="IVM62_000513"
     CDS             19603..19833
                     /locus_tag="IVM62_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844501.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            20034..20399
                     /locus_tag="IVM62_000514"
     CDS             20034..20399
                     /locus_tag="IVM62_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HAP6844502.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            20399..22126
                     /locus_tag="IVM62_000515"
     CDS             20399..22126
                     /locus_tag="IVM62_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP6844503.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22156..24072
                     /locus_tag="IVM62_000516"
     CDS             22156..24072
                     /locus_tag="IVM62_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6844504.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24757..24939
                     /locus_tag="IVM62_000517"
     CDS             24757..24939
                     /locus_tag="IVM62_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844505.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            24966..26663
                     /locus_tag="IVM62_000518"
     CDS             24966..26663
                     /locus_tag="IVM62_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6844506.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26688..26819
                     /locus_tag="IVM62_000519"
     CDS             26688..26819
                     /locus_tag="IVM62_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844507.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            26911..27774
                     /locus_tag="IVM62_000520"
     CDS             26911..27774
                     /locus_tag="IVM62_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844508.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27805..28347
                     /locus_tag="IVM62_000521"
     CDS             27805..28347
                     /locus_tag="IVM62_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP6844509.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            28361..28783
                     /locus_tag="IVM62_000522"
     CDS             28361..28783
                     /locus_tag="IVM62_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP6844510.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28713..31112
                     /locus_tag="IVM62_000523"
     CDS             28713..31112
                     /locus_tag="IVM62_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP6844511.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31144..33135
                     /locus_tag="IVM62_000524"
     CDS             31144..33135
                     /locus_tag="IVM62_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP6844512.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33158..33409
                     /locus_tag="IVM62_000525"
     CDS             33158..33409
                     /locus_tag="IVM62_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP6844513.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33399..34628
                     /locus_tag="IVM62_000526"
     CDS             33399..34628
                     /locus_tag="IVM62_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP6844514.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34625..36706
                     /gene="topB"
                     /locus_tag="IVM62_000527"
     CDS             34625..36706
                     /gene="topB"
                     /locus_tag="IVM62_000527"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6844515.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            36855..40775
                     /locus_tag="IVM62_000528"
     CDS             36855..40775
                     /locus_tag="IVM62_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP6844516.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40776..41720
                     /locus_tag="IVM62_000529"
     CDS             40776..41720
                     /locus_tag="IVM62_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844517.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41724..41927
                     /locus_tag="IVM62_000530"
     CDS             41724..41927
                     /locus_tag="IVM62_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844518.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42227..42463)
                     /locus_tag="IVM62_000531"
     CDS             complement(42227..42463)
                     /locus_tag="IVM62_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6844519.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42646..43017)
                     /locus_tag="IVM62_000532"
     CDS             complement(42646..43017)
                     /locus_tag="IVM62_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844520.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(43014..44342)
                     /locus_tag="IVM62_000533"
     CDS             complement(43014..44342)
                     /locus_tag="IVM62_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6844521.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44303..44632)
                     /locus_tag="IVM62_000534"
     CDS             complement(44303..44632)
                     /locus_tag="IVM62_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6844522.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(44890..45261)
                     /locus_tag="IVM62_000535"
     CDS             complement(44890..45261)
                     /locus_tag="IVM62_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6844523.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45406..45591
                     /locus_tag="IVM62_000536"
     CDS             45406..45591
                     /locus_tag="IVM62_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6844524.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            46103..46321
                     /locus_tag="IVM62_000537"
     CDS             46103..46321
                     /locus_tag="IVM62_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844525.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            46534..47193
                     /locus_tag="IVM62_000538"
     CDS             46534..47193
                     /locus_tag="IVM62_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP6844526.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47196..48539
                     /gene="vanS"
                     /locus_tag="IVM62_000539"
     CDS             47196..48539
                     /gene="vanS"
                     /locus_tag="IVM62_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6844527.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            48710..49516
                     /locus_tag="IVM62_000540"
     CDS             48710..49516
                     /locus_tag="IVM62_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP6844528.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            49534..50361
                     /gene="vanW"
                     /locus_tag="IVM62_000541"
     CDS             49534..50361
                     /gene="vanW"
                     /locus_tag="IVM62_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP6844529.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            50358..51329
                     /locus_tag="IVM62_000542"
     CDS             50358..51329
                     /locus_tag="IVM62_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP6844530.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            51322..52350
                     /gene="vanB"
                     /locus_tag="IVM62_000543"
     CDS             51322..52350
                     /gene="vanB"
                     /locus_tag="IVM62_000543"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP6844531.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            52356..52964
                     /locus_tag="IVM62_000544"
     CDS             52356..52964
                     /locus_tag="IVM62_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP6844532.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT        15263 a        11861 c        13957 g        12215 t
ORIGIN      
        1 tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga
       61 caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac cgaaaaatac
      121 aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc
      181 gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttcctcaa aattttaggt
      241 tatcgctgaa agccccggaa atacgggcat ttcgcaggat atgagaactc aatttactac
      301 caatttacta ctttcggatt gagccgcata tatgctgaac catactgcta atacccgcca
      361 ccccaaatac ggcacatcat aaattgaata cgacaccgta aacgcggcgt tcctctttcc
      421 cgtcatggga gaacaggacg ccgctttttt atgcccaaat agagaaagga ggcgacgcca
      481 tgctatttac ttccggccct gaccgggcgt ttgaacggct gatgcaggaa aagcccggtt
      541 tcgatcacta cgacggcggc ggggcggacg cgccggagga ttgcggcacc tgccgatttt
      601 accgcccgga ttggaaatat caattctgcc agtttgcaga gtgtccctat cagccgggca
      661 agctgaccga ctatgcagcg gtcaaattca cagtgaaaga cctgctaata aaagtcaagt
      721 agaaatttca gatttttagg gaggcgaaaa aatgcaatac aaaatcaagc cgctgagcat
      781 ctcaaaatta aaacggcggc cagccagatg gtatagagtg taaaaatcag tcgtgctcca
      841 gggattccaa gtaggctttc acagaagcgt agtagatgcg caggaacttg ttggcagagg
      901 ccatcatgta gacacgatat ggcttgcctt cagagcgttt cttgtccatg aactggtaca
      961 ccggctcgtc tattggagca cattgcagga tgacgctcat caccagaaag agcgtcctgc
     1021 gcagtgaggc agatcctcgc ttggaaatgc tacggctacg gacatctatt tgaccggatt
     1081 ggtagggcgg ggcgtcaata cccgcaaagg caaccaacgc ttttttggaa tgaaaacggc
     1141 gtacatcgcc aatttcagcc aggagttgag ggccgagggt tgggccaaca ccaaacatcc
     1201 ccatcactac aggatactcc ggcagagaag ctgccaggga ttgcatctcc tgtttgagag
     1261 cagctaacgc ggaagaagtt gtctggagtt gagaaatggc ctgttctacc aaaagttttg
     1321 ccgtatccgt tttcggtatg acaccgaagt gtctgcaagc agaggcataa atatccagcg
     1381 ccttatcctc gctgaaattg tagccgtgct ttctgcacca cttctggtat tgggtagtaa
     1441 aggccttctc agaccggcca caaacgcact cgcaatgcca aaaagtagcg acaaagtcca
     1501 cccacttctc gctgccatcg gcgcggggcg gactggtaaa caagcgattt gcgtctggga
     1561 aggtggtgtc cagcagggag atcaggttat tcttcagcat ggtctgaact ttggaatact
     1621 gttggtactg ccggtagcag attttcagca tgagtcgggt atcctcctcc gggatatatc
     1681 tcggcaatgt gagccagtgg tcaagaccgt agttggccag ctttacggca tcctttttgt
     1741 cggttttggc ccgtcttaaa ctgttgtttc cgtagtcatg caccagcatt gcattgacta
     1801 ccgagacata aaggcccgcg tcgtggagca gccaggccac cggagcatgg taattacccg
     1861 tggattccat caccacacgg gtctcgccgt ccaggctttt gagcagtcct gccagctcgc
     1921 tcagttcatt ggcggtgtga cgtacttcaa agggtgaaac cactacctct ccgaagggcc
     1981 gcatgactgc aatcatgctc ttacccttgg aaacatcgat gccaacgcag ttcattcaca
     2041 ttcctccaat acaaagaatc tgcaaccgga atccatcttt tctcgctgcc gattcaatct
     2101 attgtgtgac acgaactctc cgacatccgg tggttcaacc tgcttaaatc gaacgctgca
     2161 acgagaggat ggctaacagt ctttcacacg gacatacaag cccaaggagt gattggtcag
     2221 ccagttgctc ctctcattgt agcttaggca cgagatggag ggaaagccgg actggctgtc
     2281 cggctttcct gtccaaattt attgtaatag gagcgaacaa cgctatggca gttttccgtg
     2341 tggaaaagaa caaaggctat acggtgatgt caaatcacca cctgcggaat aagaacctga
     2401 ccttaaaggc caagggcctg ctgtcgcaga tgttgtcctt gccggaggat tgggactata
     2461 ccctaaaagg actgtcctat atcaaccggg aaagcatcga cgcgatcagg acagcggtat
     2521 gggagcttga aaaagctgga tatatccggc gtgagcaggg ccgtgacgca aaaggcaaaa
     2581 tggccgatat ggtctatacc atctacgaac agccggtatt ggaaaacccg gtattggaaa
     2641 atccaacatc ggataacccg gtgttggaaa atccgacatc ggataatccg gcgtcggaaa
     2701 atccaacgca attaaataaa gaagtacaaa gaactgactt atcaaaaaaa gaaaaaataa
     2761 ttacagatgg acaaagtacc cattccattc ctatcccatc cccgacccct gcccctatgg
     2821 agggcgaggc agcggctacg ccgccggaac ggaaaaggac aggaaaggat gaggctgtac
     2881 ggatttatcg ggaaatcatt ttggagaaca tcgagtatgc ctatctcatt caggacagga
     2941 gcattgaccg tgaacagctt gacgaaatcg tagacctgat gctggaaacc gtctgcaccg
     3001 cccgaaagac aatccgtgtc gccggggacg actaccctgc cgagctggtg aaatccaagt
     3061 acatgaagct gaccggcgaa catatccgct ttgtccttga ctgtctgcgg gagaacacaa
     3121 cgaaagtccg caatatcaaa caatacctgc gggctatgct gttcaacgcc cccagcacca
     3181 tttccaacta ctacacggcc cttgtcgcgc acgacatggc acagcccgat tggggaaagc
     3241 ctaaatccgg cctgccggac tattcctgcg gcgagggcga aagcctgtaa cgagaaaagg
     3301 aggacgaccc tatgaagcag ggcgcattga tttttgacga gcgaacagac cgttacgaca
     3361 tccgctttga tctggccgac tattacggcg gcctgcattg cggggagtgt ttcgacgtga
     3421 tgatcggcgg caaatggagg cccacccgca ttgaaatggc cgaaaactgg tatcttgtcg
     3481 gcatccgggc cgacgacctt tccggcctgc gggtgcggat ttgacctata tctacctgcg
     3541 gcgtctggtg gttccgcctt agacgccgct tttgactttc tgacaaggag ggatgcgatt
     3601 gcaggaagaa gttactcaaa agaccattgc cctgtccatg aaaacgggca agctcacggc
     3661 tcaagtgctg caagcggcgc tgaaaaagta cctgcaacac cgggccaagg gcaaaaacac
     3721 gctgcaccac ggccagcaga gcctaaagca gcttaaaaag catggggctg atctctccaa
     3781 cattgagatc accgaggcca acattggagc gttcaagcca tgcgccaaga aatacggcgt
     3841 ggattttacc ttacgaaaag ataagaccac ccagccgccc cactacgttg tgattttcaa
     3901 ggccaaggat gcagataacc ttgatctggc gtttcgggag tttaccgcca agacgctttc
     3961 caaggagcag cggccctcta tccgcaaggt tttgtccgct gtgaagcaaa aggcggcggc
     4021 ccagaccaag cagcgggcca aggagaaaat caaggaaagg gggctggaac tgtgaagcct
     4081 gacgtaaaga aattgctgat cctgaacctc ccgtatctga tttttgtgta cctgtttgcg
     4141 aaatgcgggg aggcgtaccg gctggccgct ggtgctgacc tgtcggggaa gctcctgcat
     4201 tttgcggatg gttttgcggc ggcttttgcc aacccgctgc caagcctgca cctgtttgac
     4261 ctgtgtatcg gtatcgccgg tgccgtgatc gtgcggctgg tggtgtactg caagagcaag
     4321 aacgtgaaga aataccgcaa aggcatggag tatggcagcg cccgctgggg gacggccaaa
     4381 gacatagcgc cttacatcga ccccaagttt gaaaataata tcctgctcac ccaaacggaa
     4441 cggcttacca tgaccggcag gcccaaagac cccaagacag cgcggaataa aaacgtgctg
     4501 gtgatcggtg gcagcggttc agggaaaacg agatttttcg tgaaacccag tgcgcccgta
     4561 agggcggtat aaatcctacc ttgagcaagt agtaggtaaa agtatcaagc ggcattgtgc
     4621 cgcaacctat cattccccgt cttaccccaa aagggaaacc ggagggcgac catagcatga
     4681 cggaggacac ggggcgctga agcctcagaa gcgccggcgt gacggaatga aaatccacgt
     4741 atgaggatgg aagctagact gcttgaatga cagcctgaaa tcgggaccaa gagcgtaccc
     4801 ctgacgtaga gaggttgtac tcgtcagtgt tcctgacagt cgcattgttt ggctatgcgg
     4861 ctgctgatac tcggagcagg ttcagccacg ggaaagtgga aaaaggcgaa cgtcacatac
     4921 cgacaacgtg gaacgcttgt ttataccgta ctaaacgggg attgcctaaa gtgaaatgcc
     4981 gaaaggctat gaaaacgctg aaatgcgggg ttctgaatat tcaccatggc aacagagttc
     5041 ccatagtagt ctgcggacgg gaaagccgtc tacatggcaa aggggaacag tgaatcattt
     5101 tcaaaacaga aaggatggtg tgtgagacac tatgagaaat ccgattcatg tcttgaaaag
     5161 ccttgaagaa aaggcaagtg taagtaacta caaatatgaa agattatatc gcaacctata
     5221 caatccagag ttctatctcc ttgcatatgc gaacattgcg aaatcgcagg ggagcatgac
     5281 gcagggggta gacgggcaga cgctggacaa catgagcctg ccgagaatta accggattat
     5341 tgaatccata cgcaacagga catatcagcc aaagcctgca aaaagaaagt acattcctaa
     5401 aaagaacggg aaacttcgcc cgttgggaat cacttctact gatgataagt tagtgcagga
     5461 agtggtcaga atgattcttg aagcaatcta tgagccgaca ttcagcaaca attcacacgg
     5521 tttcagacca aaaagaagct gtcacacggc acttacgcaa gtgaagaaaa acttcacagg
     5581 tgttacatgg attgtcgagg gagacattaa ggcgtgcttt gataacttcg accatcatgt
     5641 gttggttgaa ttactgcgga aaaggatttc agacgaggct tttatcggtc taatctggaa
     5701 gttcctgaaa gccggatata tggaacaatg gcagtacaac tgcacctact ccggtgttcc
     5761 gcagggcagc ggtatcagtc cgatatgtgc aaacatctac ctcagcgaac tggacaacta
     5821 tatgcaggaa tacaaagaaa agtatgattg cgagccagaa cgcagaagga cgaccagaga
     5881 atacgagcgg gcgtcccgga gatacagaaa ggcacgtaaa gcgttaatgg gcgcagaaaa
     5941 atcaactcct gaactggtaa aagaatttaa ggattccaga aggaagaaga tgaatcagca
     6001 ctattacaat ccgtttgagg aaggcttcaa gaaaatccag tacaaccgtt acgccgatga
     6061 ttttgtaatc ggcgttatcg gctccaaaaa ggacgcagaa aaaatcaagg aagatgtaaa
     6121 aatctttctc caagaaaaac tgcatttgga aatgtccgag gaaaagacga aagtcaccca
     6181 ttccagtaag ccggtacgct atctggggta cgatttcaaa gtaatccatt ccaagaacat
     6241 gaagcgttgt aaaaacggcg acatgaaacg ggtgtggtat ggaaaagtat tcctgtatat
     6301 gccgaaagaa aaatggatta aaaaagcgat ggaacgggga gcgatacagg tgaaacgcaa
     6361 caatgacaca ggcaaagaaa tgtggcggcc tatgccaagg aaagacctga tgaaccgcag
     6421 cgacgcagag attgtgtcta ctttcaattc cgaaattcga gggttatata acttctatcg
     6481 gatagcagaa aacgtaggcg cattgcacaa gtattactac atggtgcggt acagcatgtt
     6541 aaaaactctg gcaggaaagc acagaacgaa tgtcagtgtt attaagaagc ggcacatggt
     6601 aaacggagta ctgagaatcc catacgatac aactaaggga cgtaaatact gcgaatttta
     6661 ccatgatgga ttcagaaagc acagcgacgg ctatgacaat gtggcagacg ttatgccgag
     6721 ttataggaaa tatgacagca ggcacacgat agtaaaccgc ataaaagccg gagtatgcga
     6781 gatttgcggg gaacatgcag actatttatg tatgcaccac gtaagaacgc tgaaatcgct
     6841 gaaaggcagg gatatatttg agcagaaaat gctgaaaatg agaagaaaat ctctggctct
     6901 ctgccctgac tgctttgaac tcttacacga aaccaaagaa tcaaggtgat tcatggaaag
     6961 ccggatacgc tgagaggtgt acgtccggtt tgggaggcgg ctccctgaaa cctactgatg
     7021 aaagtcagca aggcgcaggg tgcctacctc atacctgatg caatgctttc cgaccacgga
     7081 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
     7141 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
     7201 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
     7261 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
     7321 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
     7381 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
     7441 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
     7501 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
     7561 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
     7621 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
     7681 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
     7741 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
     7801 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
     7861 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagct
     7921 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
     7981 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
     8041 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
     8101 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
     8161 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
     8221 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
     8281 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
     8341 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
     8401 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
     8461 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
     8521 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
     8581 caaaatcgca gggtgtcaag cacttaatgt cttctatgtg aaaagatttt aaagagaaaa
     8641 tgcacatcct acgcagataa catgacaatt tcatgcaaag cgaaaggaga caccatgaaa
     8701 atcgaataca caaaagtggg agactattat ttaccgaatc tttactaccc ggaggaaacc
     8761 agaccgattg gcttatgggg aatgcttcgg aaggaatatc tgaaagaaca taaatctgga
     8821 acttatacat atcttcttct gaccgccagg ctggacagtt atttggcaga tctgaatgaa
     8881 caggcacagg aacgctttga attgatcgaa actcagatgc gaagtgcgga gggagtttcc
     8941 gaggaactga agagacagaa tcctatggaa tggctatgcc actgtaacaa tatccgcaac
     9001 cgggctgctg agatcatcaa gcaggaactg atttatgtat aagggggatt ctagatatga
     9061 gaagagaact gattgaaagt tattatgaag aataccagcg ggagctggaa agatttcttg
     9121 aatctgaccc ggagatgttc actctggaag cagaaattca tcattacata gaaatactgg
     9181 aaccggtcct cagagatggc ggacaggaga tgtggcagac actggacagg attatttcag
     9241 cgaaaaatgc aatggaggga attgtggcaa aagaaatgta tatccgggga tttctggatt
     9301 atgaaagact ggtgtgtgaa aaagacagta agatctgaat aatcagaaca tagactatat
     9361 ataacgaggc ttttattgta tagacagtaa gagcctcatt taatatagtt ttcataataa
     9421 aaaacaggtg gtagtacgta tttgctttgt gttatataag aattggtctg gctgaacaat
     9481 ggtgaaaatt tactatgatt ttacttaaat ctgattgaag gatgaaaaag tgatgatatc
     9541 atatagtggg ttgatagggt tacagaaaat gtattcacag aggcagagaa gaaataatga
     9601 aagtaaaaaa tgcagatgaa ttttataaaa tattagaaag aaaggctata tatccggtat
     9661 ttcagccgat tgtaaattta cagactggag atgtggcagg atatgaagct cttagtcgta
     9721 tcgacaggca tgatacgact cttatgattt cagatttgtt tgtgattgct gaacaggtag
     9781 gctgtgtttg gaaattggaa aaattgtgca gaaataaagc attgaaggca gcggcgaata
     9841 aaccagaaca tgcaaaatta tttcttaatg tagatggtaa tatcattcag gataaatcgt
     9901 tcatacaggg atttacaaac cgtaaggctg cgaaagccgg agtaccgtcc tgtgacattg
     9961 tatttgaaat aacggaacgg tcggatatag aaaactatca gattctgcaa cagattatga
    10021 agcattatgc agatcaggga tatgaaatag ctttggatga tgttggagct ggatattctg
    10081 gtctgaaccg tgtagtaaat acatcgccaa actacttaaa agttgatata gaactggtca
    10141 gagatattca aaaacataag aaaaaggaaa ttatgatgga atttctgttg cattactgta
    10201 atgagacagg agcaattctg attgcagaag ggatagaaac agaagcagag ttggagtgtc
    10261 tgtgtagatt aggtgttcat tacggacaag gatattttct tggaagaccg gacagaacgt
    10321 tcaaaaatgt aaggaatgag gcgatgttgg tattacgtag actgaataat aaatcgtaat
    10381 gaaaactata tgtcagcagg tgaaaaatga ataagtcaat tataacatta taaagatttc
    10441 cattgatttt tacacctatt ttacacaagc actcacatag atttgatttc tgtcttctat
    10501 aattgtatag gaaaatgtag atcttatata ataacaggag aggaaagaga aatgaacgag
    10561 aatgtgaaag tggtttttgg tctgatcggt ggccttgcct tgttccttta tggaatgaac
    10621 agcatgagtg atgcacttca gaaagccgca ggagaacgaa tgaaaaaaat ccttggattt
    10681 cttacaagaa atccaattat gggagcactt gccggagcac ttgtaacagc tgtattgcag
    10741 agcagttcag cgacgacagt tatggttatc ggttttgtca gtgcgggact tatgagtctt
    10801 ccgcaggcaa tttctgttat ctttggtgcc aacataggaa ccactatgac ggcacagctg
    10861 atggcattta aaatcagcaa ttatatttat ccaattatct ttgttggatt tattctgaac
    10921 tttgttagca aaaaagaaaa ggttaaaaat atcggtatgg taattttttc attcggactt
    10981 ttatttgaag gaattgaaat catgggtgaa gtcatgaaac ctttggcggg aagtccggtc
    11041 tttgttgatc tgatgggaaa agtttcatcc attccggttc ttggtgttgt actgggagcg
    11101 gtcatgacat tggttgtgca gagtagttca gctacgattg cagttctgca gaattttgca
    11161 tcacaggcag gaccggacgg agtaagcagt gtaatagggc ttaccggtgc gattccgatt
    11221 cttcttggag ataatatagg tacaacgatc acagcacttc ttgcatcaat tggacaatct
    11281 aaaaatgcaa aacgtacagc aattgcgcat agtattttta atatcagtgg aagctgcgtt
    11341 tttattttcc tggttccgtg gtttgcaaaa tttgtccagt ttatttcacc aaagggaaat
    11401 gagattgatg tgatttcaag acagattgca aatgcacata caacgtttaa tatcgtctgt
    11461 acactggtct ggctgccact gattccgctg atggtaaaaa ttgttacaac aatcatccgt
    11521 ggaaatgaca aaacagagaa agcagctttt gaacccaaat atctggatat gaaagtgatt
    11581 gaacagccgg cggctgctat ggttctggta tctaaggaac tgaaccgact tggagaactg
    11641 gcagaatctc tgttaagtga tttaaaaacg gcgattgtag cagatggaga ctcaaaaact
    11701 catggttcgt ttatagaaaa tctggagatt gtacatcagt tacaggacag tgtgacagaa
    11761 tatattacca gattattcgc aagcggaaac cttacagaac aacagtctga gcagacggca
    11821 gggttactgt atgtaaacaa tagtattcag aggattgcag acagatgtga agatattgac
    11881 cagatatgtg aaaaagtaaa taacaatgga aaaagactga ccagtgaagc ctcctcagaa
    11941 atggaagact gcattgatat tatccagcag cttttaaaga aagcaatgga ggcaatccgt
    12001 aagggtgatg ctgtagtggc tgaaacagtt tttaaaaata agaaaaaaat gcataaagca
    12061 gagaaaaaat acagtaaggc acatttaaac cgtgtaaaaa atgaggtttg tgatgcgtct
    12121 atgacaagat atttttctgg gatcatgtat aattttgacc gtatggcaga taattgtgtc
    12181 agcattgcag aagaagcctg tgataatgtg gcttttataa accttgatga agagacggga
    12241 aaatcaatga tggaaagagg tgcagtataa aatggtaaag aaaaaaaaca tccgaaaatc
    12301 tgcacttctg ctgataatga taatgctggc aacgggaata acagcatgtg gaaccgagga
    12361 agagccattg ccggatctgt ctgctatggg agctgtcaca gctgtatcac gagaagaagg
    12421 gtcaggaacc agagcagaat ttgagaatct tttgaaactg cctgagagtg atacaggaat
    12481 agttgttgat tctacagaga aagttctgaa aaaggtggaa gaagataaaa acgcagttgg
    12541 ttatgtcgca tatagttccg caacagatac gaatggaaaa atacttcaaa tcaacggagt
    12601 tcttccttct gagaaaacaa tagacaataa cagttatcct ctttgcaggg attattatct
    12661 ggcttataat ggagaactga cagatgtgga acaggatttc ctgacttatg taaagagtaa
    12721 aggacaggat attgtgaaac aatattgtat tcaggctgac agtacaacaa cattcctttc
    12781 tgacaagtca gaaggaaaga ttctgattga aggatccact tcaatggaac caatggtaaa
    12841 agcactggca gatgactatc agaaacaaaa tccaaatgct gaaatagaag taaaagcaac
    12901 agattcttca agagggatta cggcggtgat aagtggagag tgtgatttcg caatgtcatc
    12961 cagagaatta aaggattatg aagcagagct tctggaaaca aaagtcattg gaaaagatgc
    13021 tatagctatt gtgataaatg aagaaaatcc actggaaaat cttacaataa aacaacttac
    13081 aggtctgtac aatggaacat ataaaaactg ggatgatctt tcctgagaaa aacgaatata
    13141 atatcttaca agcactgact ataacggtcg gtgcttcttt tttggatttt ttcaaaatta
    13201 tctgacattc ttcaaattcc cacgtgtgat tagtggaaaa ataatttttt ctgcaaaagt
    13261 accgtccaaa tgccctctga aacttgctat tagtgaggga atatttcatt tatgcgaaaa
    13321 aaacttgcaa aagggggttg gtaaataccg cttcccaggc tttataggta gaggaacaat
    13381 tattttaaaa attttacccc aaagatgtcc gcttttgcct gtctcagtac agagtatgtg
    13441 aagggactaa caaaagtaaa agaaaaaatg ataaaaatgc aaaaaaatat cacgaatctt
    13501 ttgcatcttg cgaagtcatt tgactctagt aatcagagga cgttttggaa tgttccttcg
    13561 atgaaccttg acaaccgata agtgcaccct agaagcctaa caggagttat gccatgacct
    13621 ttctttttct gagagcgagc gaaattataa tgacactccc gatgatgggc ggctgaccga
    13681 agccttatgg tcagtgacct gctgatgcag gcagaggcag tctgagacgg ctgcaggtga
    13741 cagggaaacg cacgccagac cgccggacga atgaaactga tgacaatgtt tacagggcaa
    13801 aatgcccgga aaggagcagt ttatgacaga actgaaaatg aaagaaatgc agttgattaa
    13861 ctggtttgga tgcccgaatc tgatttatac aagggagaga atccatctgg ttgccatgct
    13921 tacaacagat gaagaaatga aaaagaatct ttacagggta tgcagattcc ttgcaagaga
    13981 agaaatcgca tatcggtatc cgatcatgta tgaaatgatc cggaaaatat tttcaccgga
    14041 tgataccaat ccaccggaag catgtgcaat gtttatgccg gacgatgaaa cagatgatga
    14101 agaatatgca atggcagcgt agctgcggaa aggacagtaa cgattatgaa taaagtggtt
    14161 ttaatgggaa gactgacaaa gaatccggag atcaaatatg ccggaaaaga taatgatatg
    14221 gcagtagcaa gatatacact ggcggtaaac agaagatata aacgtgacgg tgaacaggaa
    14281 gcggatttta tttcctgtgt cacattcgga aagagtgcag agtttgcaca gaaatatctt
    14341 cacaagggaa tgaggattgt gattggcggt cgtatcagca ctggcaatta taaggacaag
    14401 gacgggaaaa caatttatac tacagatgtg atcgtggaag aacatgaatt tgcccagaac
    14461 aaggataatg gtgttggagc tgattcatcg gaaacaccga aaacagataa ggacggattt
    14521 atggaagcac cggaaggtga ggttccattc gactaaaccg aagtaaatgg ttggagaggt
    14581 tgccctttgt cggggcatcc tcttttccat tttacagaag acatcaaaaa ttacaggtat
    14641 tcaacctaac gaaatcgtga tataatcaaa atatgttcag atgaaaaata tggaggttgc
    14701 ttatgggcgt aagttataac agattatgga aaatgctcat agataaaaat atgaagcgga
    14761 tagagatgca gtacctcacc gggatcagtg gaaatattct tgccaggatg ggaaaaaacc
    14821 agtatgtatc aatggagacg attgagaaga tctgcaagaa gctggactgc accgttgatg
    14881 agatgctgga atttacagat gatgagtaac aagagcagac atgtcaaatt attaaaactg
    14941 gaagaatgct tcagaagtcg aaaaaagaaa ttgaaaaaag ataacacgcc ggatacggca
    15001 taaatctctt agcaacgcta agagcgcact tctatacagg caattccatc tgcctgtatc
    15061 gtgcgacctg cggtgctgtt cccgatttca agaaatctca aaccgggaaa tacagcgaaa
    15121 tcagagattt cgtacaaggg gaagatcccc ttgcggcaca gatgcctatc cctgataaag
    15181 tttgtgactg aatatttcag atatagacat ataaggggaa agaaagtgtg aaatcaaaat
    15241 ttgtggagag attttatgat tagaaaagca atactaaaag atgttaattc gattgaagat
    15301 acttacaatg agcatttcca gtatgaactg aatcatacag cctttactgt atttaagaaa
    15361 ggggtttatc caacgaaaga tgatgcagaa cgggctattt atgctggtgc tttgtttgta
    15421 tatgaagaaa acggaacaat tgttggaagt atcattattg ataaagttca accaatagaa
    15481 tatgcaacta ttccttggaa agaaaaatta tctgaagatg aggtaatggt aattcacctt
    15541 ttaatggtac gtccgagtat gtctggtaaa ggaattgcat catcactaat aaaattcgct
    15601 acagaattag cacaaaaaaa tagttgcagg gcactacgtt tggatacagg aagtcaaaat
    15661 attcctgcgc tatctttgta tcaaaaaaat ggatttgaaa taattgctag tgcttctaaa
    15721 aaagttggag atgtaattgc acacaaaaac catctttttc tcgaaaagat attgtagttc
    15781 gtaaattcca gtttgaaaaa ttgagaaaat cggagtttga ggagcatcac cgttatgatt
    15841 agagaattac ggaaagtgga tataaataaa gttgcagaaa tatggttaga tacaaatata
    15901 aagacacatt attttatctc tgctcaatat tggaaaagta attttgagtt agtaaaagaa
    15961 cttctattac aggcaactgt ctatgtttac gaggataaac aggaaataca gggttttata
    16021 gggttaagta acgaatacat tgaaggtatt tttgtttctg ccgaaatgca atctcaaggt
    16081 ataggtaaaa ttttgctaaa ctatgtaaag ggaaaaagaa acaaattaat cttaaatgta
    16141 tatcaaaaaa atacacgggc aatatccttt tatcaaagag aaggatttga gattcagtat
    16201 agcggtttgg atgaagctac tggagaaaaa gactatgtaa tggcatggca gcagaaatag
    16261 aaattcaagt tttagtgttt ggaaaattat gacttagtgt gcaagttggg agaaaatatg
    16321 gtaacggaaa aagagaaaaa agcatgtcga tttattgccg aatggctata agattgtgag
    16381 acagtctctg gtagatgatc tggagggcga aaaaaaggta tgttgggaga cagaaatttt
    16441 gcaggatgat gatagtggat ttacttatga aatcagcagg tgtctgtatt tcgatacctg
    16501 taaggaacat ggctatgcag agttctgcaa agtattttgc aaccatgact ggtatgctta
    16561 tggagtatta aagcatcatt cgagatttat cagaaaatcg acgattgccg aagatggaac
    16621 tgtatgtcat gatacaatcg agaaagtaac agagaaacat tttttataaa accgaaaaat
    16681 tgattatggg aggaaattga aaatggcgaa agaagagaga tttgaaaaaa tatattctca
    16741 gggagcatta actactactg agatatgggt ggataaggaa actggtgtga attatttata
    16801 tcatgcatgt gggtattcag gtggactgac tccattgttg gacagagatg ggaaaccagt
    16861 tatttctcag gctataaata gatgacctta gaaacagcaa ctgttatggt aagtaacagc
    16921 atcgtcacaa tatgtatatg gtcgattggt gtgtgcggta atatactata ttcagaaaaa
    16981 caattcagga gatttttatg agaagatcag gttggacaaa atggttgatc gtgattccgg
    17041 caatgctagg cattgtgttg gtgacctatg tgcggtatac aacggatatc tggggatttg
    17101 gtgcatttat ctgccagctg attgcaattt tggaattggc ttatggtctg cgaattgcga
    17161 tgctggcaca gaatagaaag aagtcctatc ggttaacacc ggaagaacga catgagtatg
    17221 cacggtattt atatgagaag caatatcacc gttaccctgc tgtagcgaat cagatgcttt
    17281 tggttatggc acggatgagt gtactcctta acaattatga acgggctgca caggagctgg
    17341 cagatattcg tatagataaa ttcaatccag cacagttgaa gttgtattat tacctgaaag
    17401 tggttacagc aatggcagca ggagatgcga cagggattca agagagccag atgtgttacg
    17461 cagggattcc ggatactaaa tgcacatatc cgtcaaaagc agagcttgct atgtggatag
    17521 agcagagagc ggcagcgcag atggcagagg cactgaagaa ggcagtgccg gataaaaaag
    17581 agcatccgat acggatcagt atcattactt tggttcttgc gtatagtacc ttcttctatg
    17641 gattgtggta tggtataaaa cgtgatatgg gatatgaagt gagatatcgt ttcgcagaaa
    17701 tatcgattac tgttattaca atcagtctgg cggttctttt gatctggggg atcgtgagtt
    17761 tatacagaca caattgccag gcattatcca tacaattcgg taaaaacaaa gtggcattga
    17821 taattgtcta tggagctgct gcgatatttg cacttttgat tattggaatg agtggtctga
    17881 gtgtagtgtt cggaatggat ggaacagaaa cggtgactgg ttgtgacagc aaatatacgt
    17941 atataactgt gcaggaagat tatggtggtg aacggagata cagaaccaat aatccatttg
    18001 ttatgcagag tatggatggt ctgtggtggt cttcggatac aatagatgaa gaagataatc
    18061 aagaagagga tactacagaa ttaccggatt tggatacaga gaattcaaca gattcagagg
    18121 atattcagag tggaaatacc atagaagatt acgagacaga agggcagcat gatggtctga
    18181 caattcaaaa tgcaatgctg gcagtttata attaccttca ggagcagaat acacttcaaa
    18241 atatggcgtt cagttacacg gcgaatgcaa agggagaagt ctatgctgtc gtcagcgaaa
    18301 cgcaggaaac aaagaacggt aatacggtaa atgtgagata ctgtctgtac gataatggtt
    18361 ccaagacaga tgcagatggt aattcctgcg aagaactggt gttagaaaaa gtatatccgg
    18421 atggcaatta cgagaccgaa ttggtagatt tttatctggt aaatgcagat acgatgcagg
    18481 tgacagacga acagaagaat acgtggtaaa ataaattctg gggaagtagg tgcattgtat
    18541 gacaatgaga gaaaaaataa aactattatc gtaaatgttt caagcggact gggttctttt
    18601 ggaagtatgc aaaaggactc caagacataa gaggtgaact gcgctgatcc aggtgccacg
    18661 gcaacagatt tgaatgtcga cggaggaaag caaacagtac aggcgcaatc gtaaaacttg
    18721 caatgattga taaaaccgga ctaacagtaa cttttattag tcgatttgga aatatgcctt
    18781 ggtaatcaac aattccggtt ttactgacta agaaaatgag gaggattcat aatgagtgag
    18841 aaaacagaga ttacttttat gcaaacaaga ctaatccggc ttgcttcaga agagtggcat
    18901 ttacctgttg aacagattat ccacttgttt aaagaagccg atgttcttgg atatatagaa
    18961 aaatgctatg ggatttttca ttgtgaaggc gatgaagctg tgcttgaaga tattactgaa
    19021 tttttgcaaa gaaaggggat agagattagt gcttgagtta aaagatggat ttgttctcta
    19081 tcatggaagt tattgtgaag taaaagagcc agatcttgcc aaatgtgcaa agagaaaaga
    19141 ttttggacag ggattttatt taaccacatc aaaggaacag gcagaaagtt ttctgagaac
    19201 ctcaatagcc aaagccatcg caaccggaac aattgaagag gggcagaagt ttggatatat
    19261 ttcaactttt gaattcaaac tttcagaaaa tcttgaaacg catatttttg aaaatgcaga
    19321 tgtggactgg ttacattgta tcgccgctca ccgaaagaaa aaaatgttta ttgaggttga
    19381 acgtgaaatg gcaaaatatg atatcattgc aggaaagatt gcggatgatg caacaaacgc
    19441 tactcttacc gcttatcttg cgggtgcttt tgggactgca ggtgataaag tagcagacga
    19501 tttctgtatc agacagttgc tgccgaataa attgaaagat caatactgct ttaaaactga
    19561 ggcagctatt gagtgtctga aatttgtgaa aggtgagaag atatggctga agtaacaatt
    19621 ccaaaggaaa aaatgaatta tacaatcgac cttctgatca ctatggtgac agatgaaatc
    19681 gcagaagaga caggcaagga cagaaacgag gtattgacag attttttgtg ttcaaagaca
    19741 ggaaaagcac tgtatgatga gaagacaaag ctgtggtgca ctggtccggc atatatagct
    19801 gaactgtata tggaagaatt aaagaaatcc tgatatggga tgtgagttta aatttatacg
    19861 acaatggtga caaagacttt cattccaagg agcaacaatt aaatatttca aaaacgctga
    19921 ggtcagcatt tcctaatcac aggacaacga cctcagtttt ttatacccag gaatcggcat
    19981 tcatgaataa gccggttctt tttgtttaca gaaaaaagaa ggagtacaag aaaatgagca
    20041 gagcaaaaaa tccaaagtat gaatccatgt cattggagga actgaaggcc aacatggaaa
    20101 agagcagaca ggaactggag gcagcaattc accacaaaaa cattctggag cagcggaaga
    20161 agctggtaga acgcagggaa cgttcccacc ggctgattgt caaaggggca gagtttgaga
    20221 aagtatttcc gctgtccaga gatctggaac aggaagacgt ccaggacatg atgaatcaac
    20281 tgcagaacag cagttacaac aagagtattg tgcggcaggc tcatatagcc gccttacaca
    20341 aagatcaaca gcagattgca gaagcagtaa aaaagacaga gaagggagat gacagttaat
    20401 ggcactgtat catttttcag taaggaatgt atccagaggg aaagggcaga tggtggtcgc
    20461 ttccgcagcc tatatttccg ggcagaggat ttttgacagt tattacaaca agatacacga
    20521 ttatacgagc aagtcaggtg tgatatttac agaaatcctg acaccggagt atgtgccaga
    20581 gcgtctgact gacagggaaa cactctggaa cgaggtggag catgtggaac ggaacaacaa
    20641 ggcacagctt gcctattcgt ttgacattgc actgcagaat gaattgacac ttgaggaaaa
    20701 tatcagactg gcaaaagagt tctgccagga gcagtttgtg gcacgtggca tgatcgtgga
    20761 tctggcagtc catgagggaa aagcggaaaa tgaagaagaa ccggataatc cccatttcca
    20821 tgtgcttgtc ccaatccgtc cttttacaga ggaaggaatc tggggcaata agcagagaag
    20881 ggaatatatt cttgatgagg atggaaaccg ggtgaaggat gccaaaggaa aagatatgtt
    20941 caatgctgtt tctactaccg gatggaatga tccggagctt ttaaaagaat ggcggcgggc
    21001 atggacagag aaagtaaatg ggaaattcag ggagtgccac atggcatcca gaatcgacca
    21061 ccgttcctac aaagaacagg ggattgacct gatcccgacc atccatgagg gatatgaagt
    21121 ccgggcaatg gagaaaaagg gaatcaaaac ggtgatcgga gaattgaacc gggcaatccg
    21181 gcagtttaac cagatgttta tttctctgaa agaatccatt cagtggatga agacagctta
    21241 tgaggaaatg aaagcagagc tggatcgcag gcagaacccg acactgctgg aaagcctgca
    21301 ggattattat gataaaaaga cacagggcag accaccactt cccaattttt atgcggagat
    21361 gaaacagaga ggtaagaatc tctccaattt acaggagttt gcaaaatcca tcagttatct
    21421 tcagacacat cagatagaaa cgatggatga cttgcaggaa cggattgagg aactgaacgg
    21481 tgttgtttct gtcagtaaaa aagaaatttc tgaaaagcgg aaacagttaa aggaacttga
    21541 gaatctggaa aagatggcag aggtaataaa aacaaatcag ccattgattg atgagtataa
    21601 ccatttctat tttcagaaaa gacgggagag atattatcag cagcataaaa aagaaatcaa
    21661 ttattaccgc aaatgtgaac gggaactgaa acaacatctg gataaaaatg gaaaagtgcc
    21721 aactgccaga tggaaacggg agaaagaaga acttcaggcg gggatcgaag agctgaaagc
    21781 agataatcag ccgtaccagg aggaactaac ttttgtgaaa aaggtacaga gctgtgcgga
    21841 tattgccaga cgtgaccggg aaatggcaga agcagatact tccgggcgtt cagaagaaaa
    21901 aagggagaag caggtgaagt tcccgacatt ttacacagtt caggcgaaag agattcttga
    21961 agaaaacggg aaagcagagc aacagccgtt gaaccagtcg gaacagaatc cagaaaagaa
    22021 gacctctctt ctgagaaaac tggatgaaaa aaagaaagag tgtgcagagc gtgatgcaaa
    22081 acaacaggca gtgaagaaaa agcgaaacta tgacatgagt ttataacagt ctgtcagcag
    22141 gacagacggg aaaggatgag caaaatgcag tatacagaag aacaaattat aagagcaaat
    22201 cagacagatc ttgtttcttt tctgagcgca cagggagaac aactagggaa aagcggaaag
    22261 gaataccgtt ggaaaaagca tgacagtgta acggtatccg gaaaccggtg gtacaggcac
    22321 agtcagggga ggggtggcta tccggtggat tttgtgatgg aattttacaa tgccacattt
    22381 ccggaagctg tcaagatgct aaccggggag gaaggcgaag gcaggaacag tacctgcccc
    22441 gccccgtccc ctgacttccg attaccagag aaagaggaaa ataatgatag aatcatcaga
    22501 tatctgacag aaaacagagg gattgaaaag aatatggtgg aggaatggat cggcagtggg
    22561 gatatttatg aagaaaagaa gcaccataat gtggtgtttg ttggcagaga tgcagacggt
    22621 attccaagat atgcccattg caggggaact ggtgaaacaa aataccgggg agatgtggct
    22681 gaatctgata aatcttacgg attctgtcac agagggacgg ataatcagtt gtttgtgttt
    22741 gaagcagcca ttgatctgct ttcttttatc caattatttc ccaaagactg gaaaaagcgg
    22801 agctacctga gtctgggtgg catttccagt gcggcactga tggcttttct ttctgaacgc
    22861 cctcagatta cttcggtttt cctgtgcctg gataatgacc atgcagggaa tgaagccagt
    22921 gagaaattgg caatagaaat cccggacgga tacagtgtca tccgtctgaa accgtcccga
    22981 aaggactgga atgaaatcct ctgtgataaa aatgcagaca ggaagaaatc aatcattgaa
    23041 acagttacaa tgaaagtacc ggagaaggaa gaactggtgc cgatgttgtg ttatgaagac
    23101 attgaacaga ccagtgtaga atggctgtgg tttccctatc tgccatttgg aaaactgacg
    23161 attattcagg gcaatccagg agagggaaaa acttattttg ccatgatgct gaccgcagcc
    23221 tgcacgaacc gtaagacttt cccgaacatg gaagagattg aaccttttaa tgtgatctat
    23281 cagactgcag aagacggtat gggagatacg attaagccga ggctggtgga agcaggagca
    23341 gacctgagtc gggtgatggt cattgatgat acagaggaag cattgacgtt atccgatgac
    23401 cgtattgaaa aagcaatccg tcagaatcag gtcagacttt taatcattga tccggtacag
    23461 gcttttatcg gtgccgatgt ggatatgaac cgggcaaatg aagtccgtcc ggtattccgg
    23521 aaactgggaa tgatcgctga aaagaccggt tgtgcgattg tgctgatcgg acatctgaat
    23581 aaatcttcag ggacacagag tacttaccgg ggacttggtt ctattgatat catggcggcg
    23641 gtgagaagcc tgctttttat cgggaaagta aagaaagacc cgacaacaag agtactgatc
    23701 catgagaaaa gttctcttgc acctccaggt gagacgatgg catttaaact tggagatgaa
    23761 gaaggattcc gctgggtggg agcttatgag atttccgcag atgatcttct ggacggaaag
    23821 gaaggaaagc cgacagaaac aaaactgcaa cggggaacga aactgatcta tgaactgctt
    23881 gcagacggaa atgcagtcac aatccgggaa ctggatgaaa aggcaaaagc acagggaatc
    23941 tcccagagaa ccatgcgaga agccagaagc cggatgaaag aagaactgga ttatcggatg
    24001 aatgaaaagc aggaaaatac cattcgatta aaaaaacaag ggagaatggg tgacggcaga
    24061 attttggagt gaacggcagg ctttatagat accctatctg ccgtctgccg tctgccggtg
    24121 caaggcaatc aaacggaaat agcaggtatc catacagaat aaggattgct gtgaatctgg
    24181 aacaaaaatg aattgtaata tggcggatat aatggtatga ttatccggta acagcaatgt
    24241 tacggggaag cacctgtgac agggtgtggc tgcctcctga tatttacaga ccggagcctt
    24301 tcggggttct gagatgaaag aaaggaggtg cgtgatatga cagcttatga gattatctca
    24361 atattcttag gcgtactcag tttactgatc gcttttggta gcctgttaat tgcgattatt
    24421 gcctttcttg atcggaagaa caagaggaaa taaaaatgcc tatcctgtct gccaacagga
    24481 taggcggtgg tcgtaagacc atttagtcaa ctcaggcggc atccactttg gagtgggtgc
    24541 ttttcctata acaaatgtac cacacattcc tcttgttttc aatgggaaaa ttaaaatatg
    24601 cagttcatct gacagggatt tatccctgtc tttattatat ccccaatttc tgaaatgtta
    24661 atacaaaaat tttaatatat gtaaccaaca gtctgggcag ttaatctgta aaaatggatt
    24721 agctgctctt tttttgcaaa aaatggaggt tttacaatgg cagtgaagaa gaaaattatc
    24781 aacatcagag tatattttga cggtgatcag gacgcaaaag aagtgtttgc agaggtgatc
    24841 gcacagaaaa tacgtgacac gaaaacaaaa gataaacttg caataatgaa agataagcag
    24901 tataatgatt atgagttcag taatctgaaa tctgcgtagg atgtgcaaag taacggaggt
    24961 atcagatgaa caacagatat atgagtaaca atatagaaag aaatgcaacc tggagagttg
    25021 gtgtatactg ccgactttcc aaagatgacg aattgaccgg agagagtgcc agcatctcca
    25081 accagagaga catactggtg aattactgtc agtcacaggg ctggcaggtg gtggatgtgt
    25141 ttcaggacga tggttataca ggattgaaca cagaccgacc tgatttgcag agacttctca
    25201 aagcctgtga aaaaggactg gtgaatcttg taattaccaa agaccagagc cgactgggac
    25261 gaaaccatgt acagacagga tttctgatgg aggagttttt cccaaagcat ggagtaaggt
    25321 atatcgcatt atatgataat gtggatacct ttgccggaga caatgagatt gccccattta
    25381 agaacgtgct gaacgagatg tattccagag atatttccaa gaaagtccat gcgtcctatc
    25441 acctgcaggc aaccaaggga aagtttacag gtgttgttcc accactggga tatcagaaag
    25501 acccggagga aaagggacat ctcctgattg acgacgagac agcaccgatt gtccggaaaa
    25561 ttttccagtg ggcggtggac ggtcatggga ttaactttat atccagagaa ctggaacgcc
    25621 agcagattcc ctgcccgtca tggtggcacc ggaaacgggg actccgcacc tattacacaa
    25681 aatgggaaaa gaaagacccg gaaaatggga aatatgtatg ggatgaatcc tatctgaaat
    25741 ccctgctgat gaatcccgtt tactgtggtg acatggcttc ccagaaaagg tactaccgtt
    25801 tcaagattgg caatcaggga gataaagctc cggaggactg gatcacagtc agggataccc
    25861 acgaagcaat cattccgcag gatgtatttg atctggtaca ggcaaagatg aaaggcagga
    25921 aacgccagac agaacaggga gaatacagca tgtttgccgg attactccgc tgtgcagagt
    25981 gtggcggtgc gttgacgttg aagaagacgc acaccaaaga ccagcatgaa gtttatacct
    26041 gttccactta tatccataag gggaaagcac attgtaccca gcaccgggtg gacgctgatg
    26101 acctgtatga tgcagtcctt accagaatcc aggaatgtgc aaaagcagtt accggagacg
    26161 gaacagaact ggaaaaccgg gtaaaggagt tatgcgagga agatacacag ggacacaggg
    26221 aaagtctgga gaagctggta tcaaagcaga aagaccgcct ggaaacgtta gaccggctga
    26281 ttgcaaaact gtatgatgac cttatcaatg acaagattac agaatctgtt tttgataaga
    26341 tgctggagaa gacacagaag gagcagacag atatcaaaaa ggaacttttc cagaatgaga
    26401 gtgtgctgaa tacggaagaa aaactggatg cacagtccca gcagtggatt gacgatatta
    26461 gcgagtatgc ggacatcaaa gaactggatg ccaatcttct gaaccggctg atcagtaaaa
    26521 tcgttatctc agaaccgcag gagaaagacg gaacagaaaa ttactgccgt actcctgaaa
    26581 cggtcacaat ggaaattcat ttcaacttaa aaccaatacc ggagcttgga accatcgaaa
    26641 gaggttctgg ctcccataaa taagaaaaat ctatcaaaat ttaatctatt ttaatctttt
    26701 caaggctcat ttcgagcaaa tattttctca taaaagacat aaaacagctt gtcgctggcg
    26761 gcggcgtggc cctgatcggt gtcactctgg ttcccctgct gtccgggctg ctgggctgat
    26821 cctggccttt gtccctgtcc aaacccttaa ctaatcgcct ccgcgtcctc ccggccagac
    26881 cgggagggcg cgggaaagga ggtgctaccc gtgattagtg aaatcattga agaatggata
    26941 aaaggcatcc tcatggacgg tatcacgggc aacctgtccg ggctgttcga tacggtaaat
    27001 gccaaagtcg gagaaatcgc cgccgacgtg ggacgaaccc cgcaggcatg gaacagcggt
    27061 attttcaata tgctgcacag cctgtcggaa accgtgatcg tccccatagc cggggccata
    27121 ctcgcccttg tcatgtgcta tgagcttatc cagatgataa cggaaaagaa caatatgcac
    27181 gactttgaca gctccatgtt cttccgctgg atattcaaat ccgcgtttgc aatcctgatc
    27241 gtaacgaaca catggaacat cgtcatgggc gtattcgacg ccacccaaag cgtcgtcaat
    27301 caaagcgccg gtgtgattat cgggcagaca tccattgatt ttaacaccct gataccagac
    27361 cttgaaagcc aattggaggc tatgagcgtg ggcgctcttt tggggctgtg gttccaaacc
    27421 cttgttgtgg gcctcaccat gaatatcctt tccatctgca ttttccttgt gacctatggc
    27481 cgcatggtgg aaatctatgt tgtcacgtcg ctggggccta tcccccttgc cacaatgggc
    27541 aacgcggaat ggcgcggcat gggccaaggg tatctgaaat ccctgctggc gctgggcttc
    27601 caagcctttt taatcatggt tgtggtgggt atctacgcgg tgctgataca gaatatcggt
    27661 tccgcttccg acatatccgg cgcaatctgg ggctgtatgg gctatacggt gctgctgtgc
    27721 ttctgcctgt tcaagaccgg cagtatatca aaggccgtat tcggggcgca ttgatcccaa
    27781 aatccaagca tacacaggag gaacttgttt atttcaacgt acaacatcat ttacgctgac
    27841 ccgccgtggc ggtatgactg caaaaacgta cagggcgcag cggaaaatca ctattccacc
    27901 atgagcattg acgagctgtg cgccctgccg gtggaacggc tggcgtccaa agactgcctg
    27961 ctgtttttgt gggccacatt ccccatgctg ccggaagctc tgcggttaat caaagcgtgg
    28021 ggcttttcat tcaagaccgt ggcttttgta tggctgaaac agaaccgtaa aagccttaca
    28081 tggttttacg ggctgggccg ctggacaagg ggaaacgcgg aaatctgcct gctggcaaca
    28141 cacggcaagc cgaagcgtcg ttctgcctct gtccatcagt ttattatcag cccgattgaa
    28201 caacacagca aaaagccgga tgtgacccgt gaaaaaatcg tagagctggc gggcgatttg
    28261 ccccgcgtgg agctgtttgc cagacagaaa acccccggct gggacgtgtg ggggaacgag
    28321 gtggaatgtg accttatcct gacgtgaaag gaggtgccaa atggcttatg taaccgtccc
    28381 caaggactta acccatgtga aaagcaaggt tgtgtttggg cttaccaaac ggcaactgat
    28441 ctgtttttcc ggggcgctgg tgatcggcgt ccccttatat tttctcaccc gtgactatct
    28501 taccaacagc gcggcggccc tgctcatggt atttgccatg ctgccgggct ttttgcttgc
    28561 catgtacgag cgtcacggcc agccccttga agtggtgatt atgcagatca tccagtgctg
    28621 cttcatccag ccaaaggagc gtccctatca gaccgacaac acctacaccg cccttgtgcg
    28681 gcaataccag atggaacagg aggtaaaggc cattgtccaa aaaggaaatc ccaagagaaa
    28741 ccgaaaaaac aaagctgacc cgcgcccagc gaaaagaaat tgacgcggta atccgtaaat
    28801 ataagggcga cggcaggccc cacacggcgc agcagagcat cccctacgag gtcatgtacc
    28861 cggacggcgt gtgccgggta tcgcccggcg tcttttccaa gtgcattgag tttgcggaca
    28921 tcagctatca gcttgcccag ccggacacgc agacggctat ctttgaaaaa ctgtgcgacc
    28981 tgtataacta tgtggacgcc tccattcata tccagttttc tttcctcaat cggaaagttg
    29041 acccggtgca gtacgccaag agctttgaga tcgcgccgca gggggacgac ttcgacgaca
    29101 tccgggccga gtacaccggc attctgcaaa agcagcttgc aaacggcaac aacggcatgg
    29161 taaagaccaa atacctgaca ttcaccatcg aggccgaaag cgtgaaagcg gcgcgagcca
    29221 gattgaagcg catcggcttt gacctgttag gctatttcaa gagcatgggg gccgtagccc
    29281 atgtgatgga cggctgggaa cggctgaacc tgctgcatgg cgtgtaccac ccggacggcg
    29341 agatattcaa ttttgactgg aagtggctgg ccccgtccgg cctctccacc aaggatttta
    29401 ttgccccgtc ctccctgtgc ttcggcaatg caaagacatt cggcatgggc ggcaaatacg
    29461 gcgcggtaag tttcctgcaa atcctgtccc cggagctgtc ggacgatatg ctggccgact
    29521 tcctcaatac ggaaagcggt gttttagtca atctccatgt gcaggccatt gaacagacca
    29581 aggcgatcaa gaccatcaag cgcaaaatta ccgaccttga cgctatgaag attgcggagc
    29641 agaaaaaggc cgtccgcagc ggttacgata tggacattct tcccagcgac cttgccacct
    29701 acggcgagga cgccaagaag ctgctgacaa agttgcagac ccgcaatgaa cggctgttcc
    29761 agttgacgtt ccttgtcctc aacgtggccg acaccaagca aaagctgaat aacgacgtgt
    29821 tccaagccgc aggcgtggcc caaaagcata actgtcccct tgtccggctg gactaccagc
    29881 aggaacaggg ccttgcaagc agcctgccgc tgggcgtcaa tcagatcaag atacagcgca
    29941 gccttacgac ttccagcgtg gccgtgttcg tcccctttgt gacgcaggag ctttttcagg
    30001 gcggcgcggc catgtattac ggcatcaacg ccaagtcccg caacatgatt atgctggaca
    30061 ggaagcaggc ccgttgcccc aatgccttga agctcggcac ccccggcagc ggcaaatcca
    30121 tgagctgtaa atccgagatc gtcagtgtgt tcttaacgac gcctgacgat atttttattt
    30181 cagacccgga ggccgaatat tacccccttg tcaaacggct ccacggacag gtaatccggc
    30241 tgtcgcctac cagcaaggac tttgtgaacc cgctggacat caacctgaat tattcggagg
    30301 acgataaccc gctggccctg aaatccgact ttgtgctgtc gttctgtgag cttgtcatgg
    30361 gcggcaagaa cgggctggaa gccattgaaa agaccgtgat agaccgggct gtgcgcgtga
    30421 tctaccggcc ctatctggca gacccccgcc cggagaatat gccgatcctg tccgacctcc
    30481 acaaagcgtt gttagatcag catgtcccgg aggctgaccg ggtggcgcag gcccttgacc
    30541 tgtatgtgtc cggctccctg aacgtattta accaccgtac caacgtggac atcggcaacc
    30601 ggcttgtgtc ctttgacatt aaggagctgg gcaagcagct caaaaagctg gggatgctca
    30661 ttgtccaaga ccagatatgg gggcgcgtca cggccaaccg aagccaagga aaggccacat
    30721 ggtattttgc cgacgagttc catttgcttt tgaaagagga acagacggcg gcctattctg
    30781 cggagatttg gaagcggttt aggaaatggg gcggcattcc aacaggtgcc acccagaacg
    30841 tcaaagacct gctttcctcc ccggaaattg aaaatatcct tgaaaacagc gacttcatta
    30901 cgctgctcaa tcaggcgtcc ggggatagaa aaatccttgc ggaacgcttg aatttgtcca
    30961 ccgaacagca gaagtacatc gacaattccg aacccggcga gggccttttg atctttgaaa
    31021 acgtggtgct gccctttacc aaccccatcc cgcacaacac ccagctttac aagattatga
    31081 cgacccgctt aaacgaggtg gcgggagtat gacccggcga cgccacaaag gaggtgaacc
    31141 accatgaaag agctgaaagc caaggcgaaa gtgacgcagg ccatgacccg cgacgggctg
    31201 acgctggaaa atcaggccac cggcgaggtg gagaacattt ccagccggga ggccgaacag
    31261 gatttgtccg cgccggggcc gggcggcacc gccgataagc tgctggaacg ggccgacgcc
    31321 atccatgacg gccataaggc caagaaagcc gcgaagcagg ccggggctgt ggccgccgag
    31381 ggggacgctg ccctgcaccg gccatccgcc cggctccaac tgtcggagga agaacgggcc
    31441 gacccggatt tacagcccta tatccgaaaa tcggacaaac gggccgataa gctggataag
    31501 gcccgcgccg ccatccccaa gaagcgcgtc ccggtcaagg aaaccgtctt tgacgaggca
    31561 agcggcaaag caaagaccgt cctgcacttt gacaaacagg acaaggggcc tccctccctg
    31621 aaacccaatc cggcaggccg tcctgtttcc gaggtttttc tattcggcca cgggaaaatc
    31681 catgaagtcg agcatgaaaa cgtgggtgtg gagggcggcc ataaggggga ggaacttgcc
    31741 gagcggcagg ccggaaaagc catccgcagc accatccggc accacaggct gaaaccctac
    31801 tgggacgcgg aaaaggccga gcgcaggcta ttgtctgcca acgcggagta tttctaccag
    31861 aaatctttgc gggataaccc ggagatcgca caggcggcca gcaaccccat ttcccggttc
    31921 tggcagaaac agcagatcaa gcgccagtac gccaaggccg cccggacagc gggccagacc
    31981 gcaggcgcgg cggcacaggg cgcggccaag acagccgggg cctccacgaa agcggccaag
    32041 gccgcagcca aagaaagtga acgggcggcc tcctttgtgg cccgccactg gaaaggggcg
    32101 ctggccgtcc ttgtgttggg gcttttgatg ctgcttatca tcggcggctt acagtcctgc
    32161 accgccatgt tcggcagcgc cggaagcggc atcacggcgt cctcttactt ttccgaggat
    32221 agcgatatgt tggccgccga ggacgcctac gcggagctgg aagccgagct gcaagactat
    32281 ctggacaact acgaggccac ccatgactat gacgaatacc attttgacct tgacgagatc
    32341 agccatgacc cctatgtgct gatttccatt ctgtcggcgc tccatgacgg gatatttacc
    32401 atcggggagg tacagggtga tttgtccatg ctctttgaca agcaatatat cctgacagaa
    32461 aacgtcgtgg tagaaacccg ttaccgcacc gaaaccgata catggacgga cgcggagggc
    32521 aacacccata cagacaccta ccaagtgccg tatgactatt acatctgcta cgtcacgctg
    32581 gaaaacttcg acctgtccca tgtgccagtc tatatcatgg acgaggaaac gctaagtttc
    32641 tattccctgt atatgtccac gctaggaaac cggcctgatc tgttttccgg cagccagtac
    32701 cccaacgcct ccaccctgac gccgccgacc tactacgacg tacccccgga ggctttggag
    32761 gatgccacct ttgccgccat gctcaaagag gcggaaaaat atatcggtta tccctatgtg
    32821 tggggcggca gctctcccag caccagcttt gattgcagcg gcttcatttc gtgggtaatc
    32881 aatcatagtg gatggaacgt gggacggctg ggggcgcagg gcctatataa catctgcacc
    32941 cctgtttcca gttcgcaggc caagcccggc gaccttgtat ttttcaaggg aacctacgac
    33001 acccccggcg tgtcccacgt cggcctgtat gtgggcaaca atgtgatgct gcattgcggg
    33061 aacccgattt cctacacgaa ccttacttca aactactggc aatcccattt ttacagttac
    33121 gggcgtttac cttaacgcca gaaaggagct ttcaattatg gcaaccaaac ttgaccgcat
    33181 cgaaaaggac atccagaaaa ccaagtctaa gatcgcggag tttcagaaac agcttcggga
    33241 gctggaaacg cagaaaaccg aacaggagaa tttgcagatc atccagcttg tgcgcggcat
    33301 gaacatgaag ccggaggaat ttgccgcgtt cttacgcagc ggcgcaatgc aggccgcccc
    33361 tgccgcaacc ccttaccatg aacaggagga caatgcccat gaagaataag accagacgga
    33421 tttcccgtat tctccccgca cttctggccg tgatgctctg cgtcacggcc tttcccgtta
    33481 ccgcccttgc aggcggcaat gacccggcac cggccccgct gccggaggct acaaccgaac
    33541 ccgctacggg cggtttggag cctgaaacag acgaaaccgg caccgccacg gacgggacag
    33601 aaaccgaggc cgaaaaagat accggccctc tgaccggcaa ggacatcacc gacctgtttt
    33661 ccgcgctgtt cggttccaag gtgagcattg ccgccacgga cgacggcatc cagatcacca
    33721 ccggcaccga caaaaaagaa cctgaacaga ccggcaccgt caccaccaac ggcggcaggc
    33781 tgaatgtccg caccggcgcg gggcttgaca agaccgcgtt cacccagctt cccaatggga
    33841 cgacggttga ggtagtcggc acagacggcg actggattaa aattctcttg ccggagcgca
    33901 tcggctatgt ccattccgac tatatgaccg tcagcgaaaa ggaagtagcg gctaccggcg
    33961 agggcggttt ttccctctcc attgacccgg aggaaattgc ttctctgctg gaactgttca
    34021 atggcggcgg ggccagcgcc gccctgtccc cggacgggaa cctgtcactg attgacgaca
    34081 tcggcagcgc cgcgaaaaat ggcaagcagt ttatcaccgt ggaaaccaaa aacggcaatg
    34141 tgttttatct gattattgac cgggacgacg agggaaaaga aactgtccat ttcttaaatc
    34201 aggtggatga agccgatctg ctgacgctga tggaggacgg ggaaaccgtc acccccgctg
    34261 ctgtctgctc ctgtaccacg aaatgcaagg ccggggctgt gaacacgaat tgcccggtat
    34321 gtaaaaccaa cctgaccgag tgcagcggcc cggagccgca ggaaccccag ccggaggaac
    34381 ccgaagcccc ggaggaagaa cccaagggcg gcgcgggcgg cctgatcgtg ttcctgctgg
    34441 tggcgctggc gggcggcggt gcggcccttt actatttcaa gttcaaaaag cccaaggctg
    34501 aaaccaccgg cagcgacgat ttgggcgagt atgattttgg ggaggacgag gatttggagg
    34561 acgaggaacc cgaaaccgag tttgacccgg aagctgattt tgtgagtgaa caggaggacg
    34621 aggaatgaca tataaacttg taatcgcgga aaaacccagc gtggcccgca gcattgcggg
    34681 cgtgatcggg gccacggaga aacacgacgg ctatttggag gggaacggct atctggtgag
    34741 ctggtgcatc ggacatctgg tgtcctttgc cgacgccgga cgctatgacg agcgtttcaa
    34801 aaaatggcgc tatgaggatt tgcccattct cccggaaaca tggcagtaca tcgtccccga
    34861 cgacaagaaa cagcagtttg acgttctctg ttcgctgatg cagcgccccg acgtgaccgg
    34921 ccttgtgtgc gccaccgacg ccgggcgcga gggggaattg attttccgtt tcgtctacca
    34981 gatggcggga tgccaaaagc cctttgagcg cctttggatt tcctctatgg aggacagtgc
    35041 tattaaggac ggctttgccc acctgaaacc gggcggcgac tatgatccgc tgtatcaatc
    35101 ggccctgtgc cgggccaagg ccgactggct gatcggcatt aacgccaccc ggcttttttc
    35161 cgtcctgtac cacaagaacc tgactgtggg ccgcgtccag acgccgacct taaaaatgct
    35221 ggtggacagg gacgccaaga ttaccgattt caagaaagag aaatatcaca tcgtccatat
    35281 cacggcgggc ggcgcggacg cggtaagcag ccgcttttcc gacgctgccg aggcaaacac
    35341 cgtcaaggcg gcttgtgcaa aagcgcaggc cgtttgcgtt tccgtgacgc gggagaaaaa
    35401 gacggaacag ccgcccaagc tctatgacct taccaccttg cagcgggagg ctaaccggct
    35461 tttcggctat accgccaagc agacccttga ctatgcccag gcactgtatc taaagaagct
    35521 gctgacctat ccgagaacgg atagtcaata tcttaccgac gatatgctgc ccgccgtgga
    35581 aagccttgtg tccggcctgt gggagctggt gccgtttgcc aagggcctga acctttcccc
    35641 gcagtttgac cgcatcctga acagcaaaaa ggtgtccgac caccacgcca ttatccccac
    35701 cgctgaattt gtgaagcagg gctttggcac tctggccgag agtgaaaaaa agctgctgtc
    35761 cctgatctgc tgcaagctct tatgtgccgt ggccgggccg catatctatg aggccgtcac
    35821 cgccacattt acctgtgccg ggcaggagtt taccgccaag ggcaagaccg tcctttcccc
    35881 cggctggaaa gagattgaac gccggttccg ttcttcctta aagaccgacg ccgacgagga
    35941 cgccgaggcg gtgcgggagc tgccggagct ttgcgagggc cagacctttg cagacgtggc
    36001 cgcctctgtc accgagcatt tcaccacgcc gcccaagccc tacaccgaag atacgctttt
    36061 gtccgctatg gaacgggccg gggccgagga tatgccggag gatgccgagc gcaagggatt
    36121 aggcacaccg gccacccgcg ccgccattct ggaaaagctg gtgcagatgg gctttgtcca
    36181 gagaaaaggc aagcagctca ttcccaccaa ggacggcatc aatctggctg tggtgctgcc
    36241 ggaggcgctt acttctcccc agcttactgc cgaatgggaa aaccggctga ccgagatcgc
    36301 caaggggcag gccgatcctg acgaatttat ggacggcatc gagacgcagg cgcgggagct
    36361 ggtgcagacc tattcctgta tcagtgagga aaagcagaaa ttgtttcagg ccgagcgtgt
    36421 tcctatcggg acgtgtcccc gctgtggtga ggctgtctat gagggcaaga aaaactacta
    36481 ctgcggaaac cgggcctgcc agttcgtcat gtggaagaat gaccgatttt ttgaggaacg
    36541 caagaaagcg ttcaccccga agatcgccgc tgcgctgctc aaaaccggca aagtcaaggt
    36601 aaagggcctc tattccgtaa agaccggcaa aacctacgac gggacggtgc ttttggctga
    36661 taccgggggc aagtacgtca actaccgcat tgaacacagg agctaaagga cagtaacaca
    36721 gcaagcatag ggagtgtggc tacactcccg gtaaaatccc ccggccgcgt ccctgccggg
    36781 ccggggattt ttgcttgttg gggaagtccc caacccccta ttttttgaaa cagaacagag
    36841 aaaggagtga ccgcgtgaac gaagtatttt ctatccaagt ccataaccgg cagtttttgg
    36901 aacagggcaa acaaggcgtc tggctggatt tgcccaccac aaccgaaacg ctgcaagccg
    36961 ccctgcggga tgttggcatt actgccgaca atccgcagga tttttttatc aacgggtatt
    37021 cctgcccgga ggacagacac cttgccattc cctatgacat ggtgctggcc gccgacgtgg
    37081 acgagctgaa tttccttgcc gcccggctgg gccagcttga cgccgccgag atcgcagagc
    37141 tgaacgccgc cctgcaaaat ccccgtggcg gctttgagaa tatcggccag atcatcgact
    37201 acaccgaaaa tgtggattac tatgtgcatc ttcccgacgt taggggggat ggacagttgg
    37261 gcgactacta cctgaaccgt tccggcatgg tggatatgcc ggaggaatgg aaagccggga
    37321 ttgatacggc gcagtttgga cgccacattg cccagcagga acaaggcgtt tttacccagt
    37381 acggctatct ggtgagaagc ggcgacgaat ggcagcgcgt ccatgagggc cagccggtgc
    37441 cggaggaata ccgggtgatg cactaccccc agccggaagc tgaccgggag gaaccgcagg
    37501 cccagcagcc ggaggcatcc gaaagccccg acaaagcgcc gcccccggtt atcccgatca
    37561 tgctggacgg catgaacagc aaagaacgca tgaaagtgat tactgaccgt ctggaaaccg
    37621 gcgtacagga gctttttgaa agtagccgct atatggctta cttatccgct atgtccaaga
    37681 tgcacgatta cagtctgaac aatacgctgc tgatcgccat gcagggcggt tcgattgtgg
    37741 cgggctataa caggtggcag gatgaatttg accgccatgt gaagcggggc gagaaaggca
    37801 ttaagatact ggccccggcc ccgttcaagg ttaagcagca gatggagaaa atcgacccgg
    37861 ccacccagca gcccgtgatc ggcgcggacg gcaagcccgt gaccgaagaa caggaagtga
    37921 cggttccggc ctttaaggtg gtgacagtct ttgacatcag ccagaccgag ggcaaggatt
    37981 ttcccatgcc ctatatcagt gaacttaccg gcgacgtgca gcagtatcag gatttctttg
    38041 ccgcgctgga acggacatca ccctatccca taagcattga gccgatttca gacggggcca
    38101 agggccgctg cttctacgca aaacagcgca ttgccatcaa cgagggcatg agcgaattgc
    38161 agaccctaaa gaccgccatc catgagattg cccatgccaa gctccacgcc attgaccggg
    38221 aggcccccgc cgaggaacag gcggcccggc cagaccgcag cacccgcgag gtacaggccg
    38281 aaagcgtggc ctataccgtc tgccagtatt acgggctgga cacgtcggac tattcttttg
    38341 gctatgtggc cgattggagc agcggcaggg agttatccga gctgaaagcc tcccttgaaa
    38401 ccatccgggc cacggccagc gaattgataa ccgagatcga cggccatttt gcccagcttc
    38461 agcaggagcg cgaggcccgg caggaacagg aggccgtcac ggttgacgtt accccggagg
    38521 cagcggcccc ggcgcaggcc gaggaagaac aggccccggc ccttgatccg gcagccgagc
    38581 cgcttgtgac tgtgctatgg agcgaaagcc cccagctaaa ggacggccag caaatgccgt
    38641 tgcatgaggc cgacgcgctg tttgcccgtc tggatgcagc atatccggca gaggcaggct
    38701 atgacaaaac cgcgttccgc attgactgta tctttcaggg cgagccggac agctacgagg
    38761 ggcggcagga ttttggagat agggacggtt ccctgatcga acatatccag agctaccacc
    38821 aattttacgc gcaggacgaa aactggaaaa agcatgtaat cgctacaacc ggcatagagg
    38881 gctgggagca ggacaaggcc catcgggata tgctgttagg cgagtttgtc ccctatatga
    38941 agctgcattg cagccttgcc caaatggaac agacggcgca gggccgctta cagtccgggg
    39001 aaaccctgac gccggaggaa acggcctatt tgggggcgct tctctcttat gtgcaggagt
    39061 gccgccccat gctcaatcag ggccagtatc agttgccgga gccgccgaaa ctggctgact
    39121 tcgaccagag cttgcaggac tacaaagcac aggtacaggc cgagatcgcg caggaggccg
    39181 ccgcagccgg tatgaccgtg gaggaatacg cggcggctgg ctatgaagca ccccaacagg
    39241 acaccttttc catctaccag cttcgaggcg gggaggaaac ccgcgactat cggtttgagc
    39301 catacgagcg tttacaggcc gcagggctgg ccgtggacag tgccaactat gaccttgttt
    39361 atacggcacc gctggataaa agcacgtctt tggaggatat ttaccgcaca ttcaacatcg
    39421 accacccggc agattttagg ggacacagcc tttccgtttc cgacgtggtg gtgctgcacc
    39481 agaatggaca ggacaccgcc cactatgtag acagcttcgg ctatatggaa gtgccggagt
    39541 ttttgcggga aaatcccctg cgtaccgccg aactgtctac ggagcagaac gaaaacatga
    39601 ttgacggcat tatcaacaat acgccgcccg aaccgcagcc ggaggaaccc gaacagctat
    39661 cttttgagga tgtggcagtt gcttccggcc agacacagga ggaacccccg gaggctgcgc
    39721 ccccgcaaac ggctgtccgc tattatccca tcaatgaggg agccgcccgc cgcgccaagg
    39781 aagccatgag cttttctgac taccggcccg gcagcgccac ggcggaatac cggcactatg
    39841 tcgatcaggc cgtggaaatt gcggagcgcc agaaaaagcg tgtggagcca gagtatcacg
    39901 aaaagataga cagcctcctt gacacctacg cccgcaagct ggccgagaac atgaataagg
    39961 gctatgagat taccgcccgc gtcccctctg tcctgattgc cgggccgtcc aatttcccgg
    40021 tacgggcgaa agaaaagcag aacgccgcca gtgaccgcaa catggaggaa tttcagtaca
    40081 ttcagggctt gcttgacaaa atccgcagca ccggcatggg tggtatcagc gccgacgatc
    40141 cgcaggccgt ttccaagctg gaaaagaaac tggaaaagct ggaagcgtcg caggagttga
    40201 tgaaggccgt caatgcctac taccgcaagc atgggacgct ggacggatgc ccccatctga
    40261 ccgaaagagg cattgaaaac ctgaaagcgg atatggcgtc gggctggcac tatgagaaaa
    40321 aaccgttcca gtcctggcag ctctccaaca ataacgccga gatacgccgg ttaaagggcc
    40381 gcattgagga attgacccgc caaaaggagg cggcctatgt gggatgggag tttgacggcg
    40441 gcactgtgga gatcaaccgg gtggccaacc gcctgcaaat cttctttgag gggaagccgg
    40501 atgctgctgt acgcgacgag ctgaaaagca acggtttccg ctggtcgccc aaggccgggg
    40561 catggcagcg gcagttaaac gacaccacaa tccgcgtcgc tgaccgtatc aagtgcatcc
    40621 agcccctttc cggcgaaaag cccagcgcct tacagcttgc ggcccgcagg gagaaagaaa
    40681 agccctctat ccgggcgcag ctcaacgcgg ccaagaccga acaggccaag cagccaccgg
    40741 cgcgggaaaa atcaaaggga ttggaggtag agtaaatgca gaagtttgaa aatgtggacg
    40801 ttctggccgc gctggaacag atcatgcggc agaacaccgc gttttatcaa agtgactttg
    40861 gcattgacaa agacattatc aggcgggatg ccgccagcga tcaggccgca gacaagaccc
    40921 tgctctggat gtcccggccc tccgggacgt actgtttccg ggaacgtgat gttttcctga
    40981 aagacacccg acagtacaac acatggaaat tttacgggga gcagacccgt gacaagatac
    41041 tggcctacgc ggtggagctg accggcacac agggcggcac tatccggggc aatctctatg
    41101 agctggacta tccgcagcat ttccggcacg tcattgaaac cgcccagccg gtgagtgtga
    41161 accggctctt ttatgagcat ggcacccgtg acatcccgga gagccagcgt tttgacggaa
    41221 gccctgaccg ggtattggga aactttttgt actatgaggc ccagccccat gacccggctg
    41281 tgctgcagga ggcgctacgg acggagcagc acagccgggg gcgggccgtc cccggcgatt
    41341 tcaaagtcca cgtcaccgct ttgcatgaca gccggattga aaccgaggcc cggcgtattg
    41401 tggacagcct gaaagatctg tccgcgccta acagccccaa taaaacgcat ttcatggtgg
    41461 agatctcccc gcactttgcg gcgattgctt ccaccaagga taatgaccgg ctctttgcca
    41521 tgctgcccta taaatccctg tgttttaccg ggatgaaagg caggcatggc gtgtatgccg
    41581 tgattaaccg ggacgagcgc cgggatgtga gtattcgcag gccccggccc tctatccgca
    41641 agcagctatc cgacagcaaa caggccgcca gcccaaaaaa ggcggcggcc agaaccaaaa
    41701 agaatgaatt ggaggtataa ggaatgatgc actttaccgt ggaggaagaa aacctgatct
    41761 gtatgtacca caatgcagac cggcgcagaa cgataaacag gctcaccgcc gtcatgccgg
    41821 ttatggatgg ggatatgcgg gcgcaggccc agcagaccct ttccaagctg gaacgcatga
    41881 cggatgccga ctatgacggc cagaagttcc acttcacaga cgaataacga acagcagacg
    41941 ccgggcgcgg cggccatgtg gtcgcttgcg tccggcgtct tttctttttg cctattccta
    42001 cgcatttaga acccggtatt gtcggggata ggatgaagta ttcgcttgcc cctgcaaaca
    42061 tgcgggagaa gccttgcagc gggcggtttt caatccctta aatgcgtagg ttgcagctat
    42121 aattagaccc ctgaaacaga tatagccgcg cagcgggtgt atctgtttca ggggtgtgca
    42181 ggtgatagcc gcaaagcggc gtaagagggt gcagccctct tgccgtctat ctctgttcca
    42241 gcgggaagct cggcaatgct tccagcagtt tcagggaaat gcgctgccgt atatcttcgt
    42301 caatgacttt cttctttttg ccgtttgcct gtctgatctc caccgtggaa agctcgttga
    42361 tgtagtcagc ataatgggcc aacacttttt ccgtggccca cctctcaccg gcaacggcgg
    42421 ctttgatcgt ttcatagtcc agcagctttt cttgtccgtc catcctgata cccccgtcag
    42481 actgattttt tctatgatac catattcgga agcatggaac cagagaaaac ggcgcttttc
    42541 tgctggtaaa ttctacgcat ttagaacagc acttcggcgg ggataggata aaacacccac
    42601 ataccccgct gaccatgccg gaaaagcctt gaaatataag ggcttttaca gctctaagtg
    42661 cgtaggtttc cagtatcttt tccgctggcg ctcggcctct ttgcggcggc gcatccgttt
    42721 ggagcaggcc gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg ccccgcactc
    42781 acagcagcgt ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg ccgcatccag
    42841 cgggaggaca gcggccttaa accagcggca cagaagcgaa taggaaatgc tctgtacgca
    42901 aatacattct tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt tgcagcactc
    42961 atgcgtcagc ttccgggcct tgcgatattg agggtaattc attcgtggaa tactcatagc
    43021 tcttgcgtcc attcctttcc cggcgtcata cgcaaaatgc cgtccacatt ctgcttgatg
    43081 atgccgtatt cctgcatctg ctttttcgct tcgccgtact tctcatacag ccgttccttt
    43141 tcctcggcca atcgcttgta ctcggctttc agggcgtaca gatcggggag ctttttcaca
    43201 ccctgtccct ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct ttcatgctca
    43261 cgccggaatt ttgccttatc cgctgcattg cggtattcca tagccacggg ccagagctgc
    43321 ttgtaggtag aaatattctt tatcagcacc gccatatccg caagccggtg ttccatagct
    43381 ttgagggtag cggcggcctc gtcgctggcg gcggtgatct cggccacttt gctttccaaa
    43441 tccgggtagg tgtctatccc atgttcagtc aggaaattca aagtctttgc ggcctgcttc
    43501 aaattgtgga gcttcgccca cctctcatag cctgccgact gctgggcctt aatgttgttt
    43561 tccagatcaa tccgcaaaga aatccccttg cgctctttgg gcgctctggt gataacgcgg
    43621 cctttgatac gctctttgat ggcttcctcg gtgtaggctt cccccaatgt cttgcagcgg
    43681 gtaaagcgtt cctgtccggg agcgcggaaa gaaataaact ttcccggctt gatctcatag
    43741 ccctgcgcct gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg gatagcggcg
    43801 tcaatggctg ctttgagctt cgctttccag ctcgtccctt tccgctgggc gtcccactcg
    43861 gcatagctct tgcccttact ctggccgggc ttcaccacgg acaatccgtt ttccttgcac
    43921 agcctgtcgc tggtgcgccg gatataggca tagctctgtt tgttggaaat gtacttgcgc
    43981 tgatttacca tatccacggc acagaaaata atgtgattgt ggacgtggcc cttatcaata
    44041 tgggtagtca gcacataggg atatttccct tgcagcactt cctcggcaag ctgcttcccg
    44101 atctcatggg cctgttccgg cgtggcttcg ccgggggcaa aggattgtat caggtggtgg
    44161 cccagattat tgcccttttg gtatgcctgc gccagcagca tttcaaattc aatgtccgct
    44221 gtttcataag agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt gtccgggttc
    44281 tcgatgtagt caagggcttt tttcagcgtt gacttgatag gatgtatctt tgtaactgcc
    44341 atatctgcgc cagcgccccc tttatatcct cgatgtcctg tgcgtacacc ggccccatac
    44401 cgttcagcct ctttgcaatc tgattgacgt tgacgccgat cttctgtatc tcggcggtct
    44461 gcgcccggat gtcgctggtg tccatgttga cgatatagcc gtcaatgagc atcttccggg
    44521 cgtaggctga aaagttcttc gtatgcagca gcgccatttt ctgttcaatc atagcccgtt
    44581 cctccggcgt cacagggacg cgcagcacga tttttcgctt tctgtttgcc atgcttcacc
    44641 gtcctttcca ttcaggggtt tggggatttc cccaacaagc aaaacggacg cggcccggca
    44701 gggacggggc aggccgtttt tccgggagtg gctacactcc ctatgcttgc tacgttactg
    44761 tttctccact aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag ccctctggaa
    44821 agaaaaaagt gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa gtaaaacaga
    44881 ggatgccagt tacacatcct ccgtttcatt cttcttcggc ttttcaatta gtatggcgct
    44941 gtcaatcgcg ccctcgatga tcggcaggta ttcttcgggg cagagtttca gcttgtggct
    45001 gaccctttgc cgcagttcgc tttcctcccg catgatctcc gggttaaagt agcgttccac
    45061 aggaagcccg caggctttaa taagctgtat gacaaccggc agactgggaa tggccccgtc
    45121 gttttcgatg ttggcaagat agcgccagtc aatatttatt ttctctgcca ttgctttgcg
    45181 cgaaacgcgc tttgcttgcc gggccgcttt cacatccgcg ccgaaagttt caaagccggg
    45241 acaatcttca atattcgcca tatcgcacct cccacttaca ttgtatattt catactctgc
    45301 ccgtggaatg ttggtatatg cacctttctt aaagtttata tttcacattt tgtttctttt
    45361 attcttcgag caaaagaatt ataatgtacc ctgtggaggt gtactatgga ctacatgacg
    45421 ttgaaagagg caggcgagaa atggggtgtg cccccacgat ggataaacta ctattgtgct
    45481 gcgggacgca tccccggcgc tgtaaagatg gcgacgatct ggctcattcc caaaactgcg
    45541 gaaaagccga ttgacaggcg tacaaagaaa ggaaaggcgg ttactgaata gtataatgaa
    45601 accggcgcat tttggatatg gttttgaaga gagagaaagc atggtatttt gtcacggaaa
    45661 aactataaga tgatagatgg caggctgctc cagaccgata aaaagtattc tcatctgaaa
    45721 cagaaagaaa agatttacac atggatgtat gaagaagcga aactttatca tgacagaaat
    45781 ggaaaatgcc cgaaacaaag acgaggaagc tgctgctgta tcagccgtat atggtagaat
    45841 tgaaaaggcc ggcatctgga ttctttatgg tgaagtctcc agacattgga taactgctgg
    45901 tctaatgcgc ctttgagtat ggggtgattg attttaatac cggggaggta cggctttttg
    45961 aggaaaatat tctgggattt acatttggcg attattacat ttcctgtaac tgcaaggaca
    46021 cggatgatga agatatgagg aatatagact tgctgatttt ctactgtctt gaacggtatt
    46081 aggactgaat ctgctgcgcg gagtgcggca agagcgaccg gagggagtac acacaggccc
    46141 tgcctgtgtg aaaagccata ccgcaaggtg cattcgcaaa aaagaggccg cgcattttgc
    46201 tgaggcttgg ggtgtcccca acaagcaaaa cgagaacgcc gcaggcgggc aagtggtgga
    46261 tcagaatatc cgtaaaggat attctgatcc attgcttgcc agtattatcg ggatgttttg
    46321 acaaccgctg taatcgaaac atcccaaaca ttaagaaggt gttaattatt attatcttga
    46381 tttaattcgt ttaaaccaat aatatagata catacaccat gaaaagaagc gagtatccca
    46441 atgacggatg attaaaatcc gaaaagaaat cgctttgaaa ctacagggaa actacagact
    46501 gttatgttat cttcttaaaa tggagggatt tttatgtcga tacgaattct acttgtcgag
    46561 gatgatgatc atatctgtaa tacagtaagg gcgtttctgg ctgaggcagg atatcaggtg
    46621 gatgcctgca cagatggaaa tgaggcatac accaagtttt acgaaaacac ttatcaactg
    46681 gttattcttg atattatgct gcccggtatg aacgggcatg aacttttgcg tgaatttcgt
    46741 gcgaaaaatg atactcccat tctgatgatg acagccctgt cggatgacga aaaccaaatc
    46801 cgggcgtttg atgcagaggc agacgactat gtaacaaagc cattcaagat gcagatttta
    46861 ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc tggcaaagga aatccgtgtc
    46921 ggcaggctga cacttctgcc ggaggatttc acggtacttt gtgacggtac agagctgccc
    46981 ctgacacgaa aagaatttga aatccttttg ctgctggtgc agaacaaagg cagaacctta
    47041 acgcatgaaa tcattttgtc acgtatatgg ggatatgact ttgagggtga tggcagcaca
    47101 gtccacactc atatcaaaaa tttgcgggcc aagctgccgg aaaatatcat caaaaccatt
    47161 cgcggtgtag gataccgatt ggaggaatca taataatgga aagaaaaggg attttcatta
    47221 aggttttttc ctatacgatc attgtcctgc tactggttgt cggtgtaaca gcaacactgt
    47281 ttgcacagca atttgtgtct tatttcagag tgatggaatt acagcaaaca gtaaaatcct
    47341 atcagccatt ggtggaactg attcagaata gcgataggct tgatattcaa gaggtggcag
    47401 ggctgtttca ctacaataac caatcctttg agttttatat tgaagataaa gagggaagcg
    47461 tactctatgc cacaccaaat gccaatacat caaatagttt tagacccgac tttctttatg
    47521 tggtacatag agatgataat atttcgattg ttgctcaaaa caaggcaggt gtgggattgc
    47581 tttatcaagg gctgacaatt cggggaattg ttatgattgc gataatggtt gtattcagcc
    47641 ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc gatcaaagcc ttagcggaca
    47701 gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc gctggagcga aaggatgagc
    47761 ttggcgcact ggctcatgat atgcattcca tgtatgtcag gctgaaagaa accatcgcaa
    47821 ggctggagga tgaaatcgca agggaacatg agttggagga aacacagcga tatttctttg
    47881 cggcagcttc tcatgagcta aaaacgccca tcgcggctac aagcgttctg ttggagggaa
    47941 tgcttggaaa tatcggtgac tacaaagatc attctaagta tctgcgcgaa tgcatcaaaa
    48001 tgatggatag gcagggcaaa atcatttccg aaatactgga gcttgtcagc ctgaatgatg
    48061 ggagaatcgt acccatagct gaaccgttgg acatagggcg cacggttgcc gagttgctgc
    48121 ccgattttca aaccttggca gaggcaaaca accagcggtt cgtcacagat attccagccg
    48181 ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc actatccaat gtcatattga
    48241 atgcagttca gaacacgccg cagggaggcg aggtacggat atggagtgag cctggtgctg
    48301 aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat tgatgatact gcgcttccaa
    48361 ggctgttcac cccattctat cgcattgatc aggcgcgaag cagaaaaagt gggcgaagcg
    48421 gtttaggact tgccatcgta caaaaaacgc tggatgccat gagcctccag tatgcgctgg
    48481 aaaacacctc ggatggcgtt ttgttctggc tggatttacc gctcacatca acattgtaaa
    48541 tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc tgccggtctt tttttgctgc
    48601 tacaggaaaa ctacagactg gctacaggga aagtacagat gcgcttgcca taataacaat
    48661 cgtaccagcc acaaatcgta gttttattgc aaaggaggca ttcgatcaaa tggaaaaaag
    48721 caactatcat tccaatgcgg atcatcacaa acggcatatg aaacaatccg tggaaaagcg
    48781 ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt tggggtggag
    48841 actggcttcc gtattggagg caacacagat accgcccatc cctgcaactc atacaggcag
    48901 cagcactgac gtagtggaga atttggagga aaacgctctt gccaccgcca aagaacaggg
    48961 agatgaacag gaatggagcc tgattttagt gaacaggcag aaccccatcc ccgcacagta
    49021 cgatgtggag cttgagcaac tatcaaatgg tgagcggata gatattcgga tttctcccta
    49081 tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt tacccgattg tcgcatccgg
    49141 ataccgaaca acagaaaaac agcaagaaat tatggatgaa aaaattgccg aatataaggc
    49201 gaaaggctac acctctgcac aggctaaagc ggaagcagaa acttgggtgg ccgtgccggg
    49261 aacgagcgag catcagcttg gtcttgctgt ggatatcaat gcggacggaa ttcattcaac
    49321 aggcaacgag gtttatagat ggctggatga aaacagctat cgttttggtt ttattcgtcg
    49381 ctacccacca gacaagacag agataaccgg tgtgagcaac gagccgtggc attaccgata
    49441 tgttggcatc gaagctgcca cagagatgta caaccaaggg gtttgccttg aggaatattt
    49501 aaaaccagaa aaatgagaaa aggatgttat gttatggaca gaaaaagatt gacacagcgt
    49561 ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa tgtgctttta tgcgggaatg
    49621 agatttgacg gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc ccatttgctc
    49681 tttgaaacgg attgcgcatt atacaaccac aataccggat ttgacatgat ataccaggaa
    49741 aacaaggtgt tcaacttaaa gctggcagca aagaccttaa acggcttatt gataagaccg
    49801 ggggaaacct tttctttctg gtggctggta cgccatgcgg acaaagatac cccctataaa
    49861 gacggcctta cggtgaccaa tggtaaactc accaccatgt cgggcggcgg tatgtgccag
    49921 atgagcaatt tactattttg gatgttcctg catacgccat tgacaattat ccagcgcaga
    49981 ggtcacgaag taaaggagtt tccagagcca aacagtgacg agattaaagg ggtggatgca
    50041 accattccag agggctggat tgatttaaaa gtgcgaaacg ataccgactg cacctaccaa
    50101 atatgggtga ccttagatga tgagaaaatc atcggtcagg tgtccgccga caaagagccc
    50161 caagcattat acaaaattac aaatggcagt atccagtatg tccgtgaaag tggcgggatt
    50221 tatgaatatg cccaggttaa acggatgcaa gttgccttag gtaccgggga aataatagat
    50281 tgcaagctgc tttatacaaa caaatgcaaa atctgttatc ccctcccgga aagtgtggat
    50341 attcaggagg agaaccaatg agaaaaagtg tgggcattac tgtttttgga tgcgaacagg
    50401 atgaggcaaa tgctttccgc gctttatcgc cggattttca tattatccct acgctgatta
    50461 gcgatgcgat atcggcagac aacgcaaaat tggccgctgg caatcaatgc gttagcgtag
    50521 gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc ggggtaaaat
    50581 acatttctac ccgcagcatc ggctgcgatc acattgatac gactgccgcc gagagaatgg
    50641 gaatctcggt tggcacggtt gcgtattcgc cggacagcgt tgcggattat gctttgatgc
    50701 tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg caacaagatt
    50761 tcagattgga tcgtatccgg gggaaagaac tgggggatat gactgtggga gttattggaa
    50821 ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc catgtgctgg
    50881 cctatgataa cagccgaaaa atggatgcag attatgtcca gcttgatgag cttctaaaaa
    50941 acagcgatat tgttacgctc catgtgccgc tttgtgcgga tacccgccat ctgatcggtc
    51001 agaagcaaat tggagagatg aagcaaggcg catttttaat caacaccgcg cgcggagcgc
    51061 ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt ggtgcggcac
    51121 tggatgtgtt ggagggcgag gatcagtttg tttataccga ttgctcgcag aaagtgcttg
    51181 accatccctt tttgtcgcag cttttaagga tgccgaatgt gatcatcaca ccccatacgg
    51241 cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg aattgtctca
    51301 attttgaaag gagtttacag catgaataga ataaaagtcg caatcatctt cggcggttgc
    51361 tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa ttgctgcgaa cattgatacg
    51421 gaaaaattcg atccgcacta catcggaatt acaaaaaacg gtgtatggaa gctatgcaag
    51481 aagccatgta cggaatggga agccgacagt ctccccgcca tactctcccc ggataggaaa
    51541 acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat tgatgtggct
    51601 ttcccggttt tgcatggcaa attcggggag gatggtgcga tacaggggct gtttgtattg
    51661 tctggtatcc cctatgtggg ctgtgatatt caaagctccg cagcttgcat ggacaaatca
    51721 ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca aattattgat
    51781 aaaggtgaca agccggaggc gggtgcgctt acctaccctg tctttgtgaa gccggcacgg
    51841 tcaggttcgt cctttggcgt aaccaaagta aacggtacgg aagaacttaa cgctgcgata
    51901 gaagcggcag gacaatatga tggaaaaatc ttaattgagc aagcgatttc gggctgtgag
    51961 gtcgggtgtg cggtcatggg gaacgaggat gatttgattg tcggcgaagt ggatcaaatc
    52021 cggctgagcc acggtatctt ccgcatccat caggaaaacg agccggaaaa aggctcagaa
    52081 aatgcgatga ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg ggtgcaggaa
    52141 acggcaaaga aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt tgatcttttt
    52201 ttgcaggagg atggcggcat cgttctaaat gaggtcaata ccatgcccgg ttttacatcg
    52261 tacagccgct acccacgtat ggtggccgcc gcaggaatca cgcttcctgc actgattgac
    52321 agcctgatta cattggcgtt aaagaggtga taagtatgga aaatggtttt ttgtttttag
    52381 atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc cacatgggat aacttcacag
    52441 gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc gtggcgtttg
    52501 ctctgcgcga ggcacaaatc catgcggctg cgcttggcta tggcttgctt ttatgggatg
    52561 gatatcggcc aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa ccggaggaca
    52621 atctcacaaa agaaaaattt taccccaata tagagcgagc cgagttgatt acaaagggtt
    52681 atgtggcctc acaatccagc catagccgtg gaagcgcaat tgatcttacg ctctaccacc
    52741 tggatacagg ggaacttgtt tcaatgggaa gtaacttcga ttttatggac gaacggtcgc
    52801 atcatacagc aaaagggata ggggatgcag aggcacaaaa tcgaagatgc ttgcgtaaaa
    52861 tcatggaaag cagcggattt cagtcttatc gctttgaatg gtggcactat aagttgattg
    52921 atgagccata ccccgatacc tattttaatt ttgccgtttc ataatgaaag tattggtttt
    52981 gtatcagatt ttggatttga taaaatctat gctggtcatg gacaatcttt tactggaaag
    53041 tggttaaagc atcttttgta atacattgca aattatagta ttcaatctgc ccagcggcag
    53101 aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc cattttgtat tttgctggcg
    53161 gcggttttga gaaatcaagg ccgccgttct tttctggcag caaaaggtgt ggcggcccat
    53221 aggaggatgc cgctatgctg ttttgctatt gccatgctct aatccacttc caccactcaa
    53281 aaaagccttg cccctc
//
LOCUS       DACCIQ010000009        51804 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCIQ010000009 DACCIQ010000000
VERSION     DACCIQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 51804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.009"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            96..674
                     /locus_tag="IVM62_000545"
     CDS             96..674
                     /locus_tag="IVM62_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844533.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(914..997)
                     /locus_tag="IVM62_000546"
     tRNA            complement(914..997)
                     /locus_tag="IVM62_000546"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(961..963),aa:Leu,seq:gag)
     gene            1380..2597
                     /locus_tag="IVM62_000547"
     CDS             1380..2597
                     /locus_tag="IVM62_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6844534.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2781..3617
                     /locus_tag="IVM62_000548"
     CDS             2781..3617
                     /locus_tag="IVM62_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6844535.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3693..4841
                     /gene="nagA"
                     /locus_tag="IVM62_000549"
     CDS             3693..4841
                     /gene="nagA"
                     /locus_tag="IVM62_000549"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP6844536.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5061..6119
                     /locus_tag="IVM62_000550"
     CDS             5061..6119
                     /locus_tag="IVM62_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP6844537.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6138..7157
                     /locus_tag="IVM62_000551"
     CDS             6138..7157
                     /locus_tag="IVM62_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP6844538.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7259..7975)
                     /locus_tag="IVM62_000552"
     CDS             complement(7259..7975)
                     /locus_tag="IVM62_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6844539.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7944..8729)
                     /locus_tag="IVM62_000553"
     CDS             complement(7944..8729)
                     /locus_tag="IVM62_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6844540.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8720..9469)
                     /locus_tag="IVM62_000554"
     CDS             complement(8720..9469)
                     /locus_tag="IVM62_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP6844541.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9767..10291
                     /locus_tag="IVM62_000555"
     CDS             9767..10291
                     /locus_tag="IVM62_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844542.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10307..11806
                     /locus_tag="IVM62_000556"
     CDS             10307..11806
                     /locus_tag="IVM62_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP6844543.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11894..12496)
                     /locus_tag="IVM62_000557"
     CDS             complement(11894..12496)
                     /locus_tag="IVM62_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6844544.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12600..13556
                     /locus_tag="IVM62_000558"
     CDS             12600..13556
                     /locus_tag="IVM62_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP6844545.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13861..14817)
                     /locus_tag="IVM62_000559"
     CDS             complement(13861..14817)
                     /locus_tag="IVM62_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6844546.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14991..15551
                     /locus_tag="IVM62_000560"
     CDS             14991..15551
                     /locus_tag="IVM62_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844547.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15544..16368
                     /locus_tag="IVM62_000561"
     CDS             15544..16368
                     /locus_tag="IVM62_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6844548.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16378..16995
                     /locus_tag="IVM62_000562"
     CDS             16378..16995
                     /locus_tag="IVM62_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP6844549.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17013..17696
                     /locus_tag="IVM62_000563"
     CDS             17013..17696
                     /locus_tag="IVM62_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6844550.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18379..19614
                     /locus_tag="IVM62_000564"
                     /pseudo
     CDS             18379..19614
                     /locus_tag="IVM62_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19622..20509
                     /locus_tag="IVM62_000565"
     CDS             19622..20509
                     /locus_tag="IVM62_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6844551.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20526..21362
                     /locus_tag="IVM62_000566"
     CDS             20526..21362
                     /locus_tag="IVM62_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6844552.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21365..21988
                     /locus_tag="IVM62_000567"
     CDS             21365..21988
                     /locus_tag="IVM62_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844553.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21985..23277
                     /locus_tag="IVM62_000568"
     CDS             21985..23277
                     /locus_tag="IVM62_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6844554.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23636..23733
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23849..24430
                     /locus_tag="IVM62_000569"
     CDS             23849..24430
                     /locus_tag="IVM62_000569"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP6844555.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24433..25740
                     /locus_tag="IVM62_000570"
     CDS             24433..25740
                     /locus_tag="IVM62_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP6844556.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26033..26521
                     /gene="purE"
                     /locus_tag="IVM62_000571"
     CDS             26033..26521
                     /gene="purE"
                     /locus_tag="IVM62_000571"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP6844557.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26505..27638
                     /gene="purK"
                     /locus_tag="IVM62_000572"
     CDS             26505..27638
                     /gene="purK"
                     /locus_tag="IVM62_000572"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP6844558.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27651..28946
                     /gene="purB"
                     /locus_tag="IVM62_000573"
     CDS             27651..28946
                     /gene="purB"
                     /locus_tag="IVM62_000573"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP6844559.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29126..29503)
                     /locus_tag="IVM62_000574"
     CDS             complement(29126..29503)
                     /locus_tag="IVM62_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844560.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29759..30487
                     /locus_tag="IVM62_000575"
     CDS             29759..30487
                     /locus_tag="IVM62_000575"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP6844561.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30487..30741
                     /gene="purS"
                     /locus_tag="IVM62_000576"
     CDS             30487..30741
                     /gene="purS"
                     /locus_tag="IVM62_000576"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP6844562.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30743..31414
                     /gene="purQ"
                     /locus_tag="IVM62_000577"
     CDS             30743..31414
                     /gene="purQ"
                     /locus_tag="IVM62_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP6844563.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31415..33637
                     /gene="purL"
                     /locus_tag="IVM62_000578"
     CDS             31415..33637
                     /gene="purL"
                     /locus_tag="IVM62_000578"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP6844564.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33622..35061
                     /locus_tag="IVM62_000579"
     CDS             33622..35061
                     /locus_tag="IVM62_000579"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP6844565.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35093..36136
                     /locus_tag="IVM62_000580"
     CDS             35093..36136
                     /locus_tag="IVM62_000580"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP6844566.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36133..36711
                     /gene="purN"
                     /locus_tag="IVM62_000581"
     CDS             36133..36711
                     /gene="purN"
                     /locus_tag="IVM62_000581"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP6844567.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36708..38243
                     /gene="purH"
                     /locus_tag="IVM62_000582"
     CDS             36708..38243
                     /gene="purH"
                     /locus_tag="IVM62_000582"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP6844568.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38259..39533
                     /gene="purD"
                     /locus_tag="IVM62_000583"
     CDS             38259..39533
                     /gene="purD"
                     /locus_tag="IVM62_000583"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP6844569.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39655..40569
                     /locus_tag="IVM62_000584"
     CDS             39655..40569
                     /locus_tag="IVM62_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP6844570.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40702..42267)
                     /locus_tag="IVM62_000585"
     CDS             complement(40702..42267)
                     /locus_tag="IVM62_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6844571.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42260..43711)
                     /locus_tag="IVM62_000586"
     CDS             complement(42260..43711)
                     /locus_tag="IVM62_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6844572.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43725..44741)
                     /locus_tag="IVM62_000587"
     CDS             complement(43725..44741)
                     /locus_tag="IVM62_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6844573.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44961..46049
                     /locus_tag="IVM62_000588"
     CDS             44961..46049
                     /locus_tag="IVM62_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6844574.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46103..47629
                     /locus_tag="IVM62_000589"
     CDS             46103..47629
                     /locus_tag="IVM62_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP6844575.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47631..48836
                     /locus_tag="IVM62_000590"
     CDS             47631..48836
                     /locus_tag="IVM62_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6844576.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49093..50070
                     /gene="guaC"
                     /locus_tag="IVM62_000591"
     CDS             49093..50070
                     /gene="guaC"
                     /locus_tag="IVM62_000591"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP6844577.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50334..51758
                     /locus_tag="IVM62_000592"
     CDS             50334..51758
                     /locus_tag="IVM62_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6844578.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT        16530 a         8825 c        11352 g        15097 t
ORIGIN      
        1 aaattaataa atggtttcct gaatataaaa atttcaactt tcttaataag ttagatttag
       61 gtatttcatc atttggaaag ccgttttttt tagacatgaa tatcttattt ttattgctat
      121 ttttcttaat tttccttcta acatatagga aatgtcaaaa ctattatgtg cgtatattga
      181 ctgcattacc gtttttctta aacctgatta tttattttgg taatacaatg ggtcaaagct
      241 ttacttacgt caatggaaat aaaagggcaa tgatttggag tagtagtaac ctgaataatc
      301 tctttacaga gttaggaacg aaactgtcac ttttttatcc aggtacttgg atagctactt
      361 tagtagtctt ggcattatta ctatgtttaa ttgttggaat ttatttaagt tttgacaata
      421 agaaaacttc tattttttta gttattttga tgataatggg attttgttca aggttaatta
      481 tgggattttc tcccacagta tgggcttccg gaatgcggac gtactatata ctttatgttg
      541 ttatcgccat cttagtacta atggcagtta aagagctaat gaaaagcatg agtgtacaaa
      601 aaaatgaatt tatgcaattt ggattaaccg tgcttggtat ttgtaccttt ataataacgg
      661 tgataaacag ataatataca aaaaatccct cgctttagta ttaggtgagg gattttactc
      721 gtttttcgat taattgtttt aacacttgca agtttctacg agtagcttgt ttccgacctt
      781 catatactca cctcaggctg tttacatgat tgtacgttct ttttgtaaag gatacagtga
      841 tatgtatata atggcataaa gaaacgttca aacaaaaaaa ctcgtgaagc aaaattaact
      901 tcacgagttg tagtgcagat gatgggactc gaacccacac gagctaacgc tcacatgacc
      961 ctcaatcatg cttgtctgcc aattccaaca catctgcgta ataataacac actaatgata
     1021 gcgaggtttt acatagttgt caagctcaaa aatgggagga cttattaaga tggagaaata
     1081 tgacggagaa ttttcaggac tgggaatgat cttaggtgta ttgattggac ttgcattcgg
     1141 acgttttttg cttggtttta tgctaggaat catttgtgga attgcaatgg attgggcagc
     1201 gaatctatgg aatgactatc atgacaatta agttctcaac gcttgtttgt gtttttttaa
     1261 tacttacgtt tttcctaaat aagtggcaaa attttctagg atggagtatg atattgaaat
     1321 agtaaataac aaaaaatttg gaaagttcta actttttgaa atgagggatt ggctaagtga
     1381 tgagagaatt tgaaaaatcc aacaaattag aaggtgtcag ttacgatgtt cgcggtcccg
     1441 ttctagaaga agcggagcgg atgcaagaag agggcattag tattttaaaa ttgaacacgg
     1501 gaaatcccgc accttttgga tttgaagcac caaacgaagt cattcgtgat ttgatcatga
     1561 atgcccgtga ctcagaaggc tactcagatt ccaaggggat cttttctgct agaaaagcaa
     1621 ttgagcaata ttgccaactg aaacattttc cgaatgtgac gatcaatgat atctacaccg
     1681 gcaacggagt gagcgaattg atcacgatgt gtatgcaggg gttgctggac aatggagatg
     1741 aagtactggt tccaatgccg gactatccgt tgtggacggc ttctattgca ttagctggag
     1801 gcacacctgt tcattatatt tgcgatgaag aggcagaatg gtatcctgat attgatgata
     1861 tcaaatcaaa aataacgagt cggacaaaag ctattgttat cattaatcct aataatccta
     1921 ctggggcact ttatccaaaa gaattattag aagaaatcgt tgaagtagct cgacaaaata
     1981 acctgattat ttattccgac gaaatctatg atcgcttagt gatggatggt ctagaacata
     2041 ttccgattgc tacattagcg cctgatttgt tcgttgttac gctgaacggt ctatccaaat
     2101 ctcatcgtgt agcaggattt cgagtaggct ggatggtcct aagtggagat aagtcacacg
     2161 tcaaaggcta tatagaaggg ctgaatatgt tgtcctccat gcgtttgtgt tccaatgtat
     2221 tgtctcagca aattatccaa accgcgctag gcggttatca aagtgtcgat gagctgctcc
     2281 tgccaggcgg aaggatctat gaacaaagag aatatatcta taatgcaatc aatgatattc
     2341 ctggtctttc agccgtaaaa ccaaaagctg ctttttatat attcccaaaa attgatacaa
     2401 agcgattcaa tattttggat gatgaaaaat tcgttttgga ctttttacat gaacatcata
     2461 ttctacttgt acatggtggt ggatttaatt ggaaccagcc agatcatttt cgaatcgttt
     2521 atcttcctaa aatggatgat ttgaaacaaa cagctaagaa aatgagagaa ttcctagcaa
     2581 cctatcgcca aaaatgattt taaaaaccaa atttttcagt aggagggcca cagacaaaca
     2641 cactgaggct ctctttctag tggagttttc ttaaataact aaagtatatt atttagttgc
     2701 aagtcttttc ttgaagtttg cgacttccta aagtaaaata cgaatagaat gcgaatacgc
     2761 atattttggg gaggatactt atggaaacgt taatcgagtc ctttggaacg atcattaaag
     2821 agattcgaaa agaacaaaaa atgacgcaaa aaatgctttc gcatgatatt tgttcacaaa
     2881 gtgtattaag tcggatcgaa aataatgagg aactgccgaa tgtattggtg atgatgcaga
     2941 tttgtcaacg gctaggggta acgattgatc atgtgatgaa tctctctcga aaaactactc
     3001 ggacaaacga taaattattt gaactgttgg atagttgttt ccaaaaacgt gattatcgga
     3061 agttagaaca agtactaaaa tcttctgata tttctgaaaa tctgtatcaa gcaactgact
     3121 ttcaacgcta ttattattat ctcggtgttt gcgaattcac gttgcgtcaa aatatctctc
     3181 agtcccttta ttatttgaaa gaagcattaa gctactctag ccaaaaagaa agaatccatg
     3241 tttctgacac agaaatacaa ctgattagtt gtatcggaaa aatatatggt gtggcaggaa
     3301 aaacggctga agcacgctac tatttgagtc gaagcatcca actgctgcat caatcagctg
     3361 atgaacgatc aaaaagtgaa ttttcgcaaa ttttttataa ttatggaaat ttcttattcc
     3421 atcaaaatga aatcgacgaa gcgttagaac aggtgaatca aggaatctat tgggctcaag
     3481 agaagaatag ctactattac ctcaatgatt tatttgtgct taaaagctta atatacaagc
     3541 gtaaagaaga accagaaaga gcagcattct atgaaggctt agcacaagct gtgaaacaaa
     3601 tttcaaaaaa tttgtaaatg gtgtagacca ttcttgactt cttctctaaa taagagtact
     3661 ttagaggtag aaaaacaaag ggagagaata gaatgagaac ttttatctat gctgacaaat
     3721 tctttttagc gtcaggtgtc aaacatgcag gctatcttga aattaccgat ggactttttg
     3781 gtgaatacac caaagaacta cctgagggag aagtcaacat cattgaccaa agcggcaaat
     3841 ggattgctcc tggtttggta gatacacata ttcacggcta tatgaatcat gatgtaatgg
     3901 ataatgatgc agaaggcatc aaagtgatgt ctgaaggtct gttatcttgt ggagtgactt
     3961 catttttacc aacaacattg acgtcaagca aagaacgttt aaaagatgta gctgagacaa
     4021 tcggcaaaat gtatcaggaa gcaccaggag ctaaaatcaa aggaatctat tttgaaggtc
     4081 ctttcttcac agaagaacac aaaggggcac aaaacccaag ttatttcggg gatcctgatt
     4141 tggatacttt ccatgaatgg caagaagctt caggtggttt aatcaaaaaa attgctttag
     4201 caccggaacg tcaaggtgta gaagagtttg tcaaacaagt gactgaagaa ggagtagttg
     4261 tttctctagg acacagtaat gctacactag aagaagcgca aaaagctgtt gaagcaggcg
     4321 caagtgtatt tgtccatgct ttcaatggta tgcgtggatt gaatcatcgc gaaccaggta
     4381 tggtgggtgc gttattatct ttagaacatg tcttttctga attgatttgt gatggccatc
     4441 atgtacatcc aaatgctgcg ggaatcctaa tggaaaaagc aggacatgat catgtcgcat
     4501 taattacaga ctgtatgatg gccggtggga tgcctgatgg aaattacaat ttaggcgaat
     4561 ttccagtagt agtaaaagaa ggaacagcac gtctagaatc tggcaactta gctggaagta
     4621 tattgaaatt gaaagaagca attaaaaatg ttgtagactg gggattagca acaccagaac
     4681 aagcagtcat gatggctagt ctgattccag caatcagctg taaaatcgat gatacatgtg
     4741 gaaaaatcgc tcaaggacgc gatgcagact ttatcgtatt gaaccctgat atgacattgg
     4801 aagcaactta tctagatggc gtggaacgtt tccacgcata atataattta agttaacctt
     4861 aaaactaaac ttcttaagtt tggttttaag gtttttttgt gttctagtaa aaatcacttt
     4921 acataagttt caaaagtttc tttaatttcc tattgttcaa taagtaacaa tgtaaagata
     4981 aaattaaaaa tgttaatacg tattagcaaa ataaaagctt tttttattgt gaataaatga
     5041 acataattag gaggagaaag atgaacttat ttgacatttt tcgatcgact ttgacagata
     5101 tgtcgattgt cagtgcaatc acttcaacag tttttattat attattaggg tttgtttgta
     5161 gaaaaaaggg gattttttct gaatctgttg gaaaaattct atcgaaagtc gtcttaacag
     5221 tggctttgcc agcactggca tttcgagctt tcatgcaaga tatcaataca gaattactga
     5281 tgcaaggaat caatgtcttg atttggggat tagttattta tattattctt attctagttt
     5341 caaaaccttt tttcttaagc tataaagggg ataagcaaac aacgttgcgt gtcctgacga
     5401 tctttggttc tacgaccttt ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg
     5461 gcgtgatgta ttcctcaatt ttcaatattg gctaccgaat ctttttgtat tcttatggat
     5521 acataaaaat gagtggcttg aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc
     5581 caatcattat cgctactttt gccggccttt tcatttggct gttccaaggg tatctgccac
     5641 aagtgtcagt tacaggtgca gaaggagaga taagccaagt agcgtttttg cgaattgacc
     5701 agactgcacc gtggctattt cagccgatgg attatttagc tggtttaagt tcaccattgg
     5761 cgtggctagc aatcggcgca acattaggtg aagtaagctt caaaaaagct gcatctgaaa
     5821 aaacttcatg gtattacagt ctagtcaaag tggtatttgt acctgcattg aatatcgtga
     5881 ttctagcagt tttgacggca acgaatattc taccagtcaa ttttgaagca ctggcaacaa
     5941 tcgttatcat gatggctaca ccagctgcaa cagtggcagc agcctatgct atcagttttg
     6001 atagggaggc acttttagcc tccaatgcat ccttactgtc aactattttt gctgtggtac
     6061 tgacacctgt atggatcgtt gtattacaaa tgattcatgg cataggttta ttttcataag
     6121 aaatggagga agaaagtatg agaatcgcat gttacggtgt tcgtccgaac gagatagatt
     6181 tttttgaaaa attgaatatt taccattatg atctcagtct ttatgaagaa ctgttgacac
     6241 atgacaatat cgtgtcagca aaggaccatg atgctgtatt gttgagggga aattgtatag
     6301 cagatgagac caatcttgca aaaatgcagg aatatgggat tcgttatgtt tttacacgga
     6361 ctgtgggagt caatcatatc gatctacaag cggcagctga ttttggtatg acagtagccc
     6421 gtgtaccatc gtattcgcca aatgcgatag cagagttatc tttgactttt gccatgatgc
     6481 ttttgagaaa tactgcgtat acgacgatcc gaacatcttt caaagatttt cgagtggatg
     6541 agcaaatgtt tagtcgtgaa atcagaaatt gcacagtagg gatcatagga acaggcagga
     6601 tagggttgac ggaagctgtt ttgtttaagg gactaggagc caaagtgatc ggctacgata
     6661 ttcatcagac agaagctgct aaaaaagtat tgacgttcca ctcgcttgaa gagttattag
     6721 ccaaaagcga tattgtcagt attcatgtac ctcatatccc aggtgaaaac gatcaaatga
     6781 tcaatgaagg atttttagct caaatgaaaa aaggtagcat cttgattaat acagcccgtg
     6841 gagagcttca agataatgaa gctattttaa gagctttgaa aagcaatcat ttagctggtt
     6901 ttgcaaccga tgtttttgca aatgaaacag aggttttttt ccgatccttc aagccatggg
     6961 agacgattcc agaccctgct atccagcaat tagtagaaat gtaccctaga gttcttgtaa
     7021 ccccgcatgt aggttcaaat acagatgagg cattatcaaa tatgatttcg acaagtttcg
     7081 agaactttcg tgaaataata gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag
     7141 cccgtcaatt aagataagaa aacatggata ttcgtcaata aaatgtttaa aaatagtcca
     7201 agtaaacagt tgttacttct gtatacttgg actattttgt atgggataat tcgatgactc
     7261 aacagtaatc tgtgagcttt ttccgcccat tctttgtcaa ttcataacag gttagcggct
     7321 ggccgatttc caaatatttg attctttcgt tcaatatatc ttgttctact aaaaaattca
     7381 aatatttctt gacggatata cgtgacagtg agcttttttg agccagttct tttgttgaaa
     7441 aaatagtatc ttcttttaga ataaatgcac ttattttttt taaagtcagt tttgacagac
     7501 ctttaggcaa atcgatgaat gatggtattt cgtgaatggg agtggtgtgg ttgaatactt
     7561 gatcgatcaa tttctgatcg gctttttcag tcgtttggat tgtctgagag aaagcaagat
     7621 acttttcgat cgccagctga aaacgttgca aagtgaacgg tttgatcaaa taatctaagg
     7681 caccgtagcg gatcacattc tttatggttt cgatatcatc tgcagcagtg atcaaaatca
     7741 ctgggatatc atttccttgc tgtctaagcc aagataaaaa atggatacca ctttcgccag
     7801 gcatgtatac atcaagcagc agcagatctg cgtttttttg tctgaaatat ttttttcctt
     7861 cttctacatt tctgaaactt tcgattacgt tcatattagg gatgctttcc acaaaagtac
     7921 gattcaattc tgcaaccatt ggatcatctt cgattatcag gacatttgtc atcattttga
     7981 tcttcctttt ttggtaattc tagatagacg attgtaccgc catttgtttg tttggaaaat
     8041 tctaaaaatc caccgtggcg tttgacgatt gaatggatga gattcaatcc aaacccacga
     8101 ttgattccct tcgtcgaaaa acctagcgta aatatttttt ctgagatttc ttctgatatc
     8161 ccgacaccgc catcgcagac ggtcacgatg aatacttcag cttctgtgtc atagtttaaa
     8221 tgaatccgga cctgtccgtt tctttcatgt ttttgtagca cggcttcttt tgcattatca
     8281 agtaagttcc ctaacacttg caaaagtgaa tggatggagt catccatctc cattttaggg
     8341 ataaaagaat cgctttctaa cagcatttca acagattgtt ctttggcttc gtttattttg
     8401 cctaataaaa aaccggcaat tgcaggtact ttgatcagac ttgagacttg gccaatctct
     8461 tttttataat tttgatttag ctgctggata aaatgggtga cttcttcata cttcttcaaa
     8521 tcaatcaaac cagaaatgac atgaagttta ttcatgaatt catgggtttg agctctcatg
     8581 gagtttatat attgtttcgt ccccccaagc tcctccatta atttcattac ttctgatcga
     8641 tttcgtaaag ttgcgactgc tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta
     8701 gaaatagttt ctatcccatt taaatacatg gattgagtcc gaacggtcgt ttgatgctca
     8761 atcgtctctt taaacaaaac gtcatataga ttatcactga tttttttcct acttcgaaat
     8821 ccaatagctg ctctgtttgg tagaaaatgt tttttgcagg tgtattgatc aagaggatag
     8881 ttttatcaga agagatggct aagattcctt catctacttc attttcgata atcgtttttt
     8941 cacttaactg ttctgctatt tcttgtggtt caagtccaag cagtactttt ttcatttttt
     9001 gggcaagaaa aacggcgcct atgatgccta cgctaagtcc aataatcaat gaaaaaacaa
     9061 tcattttttg tccatctttg acttgattgt caatcgtgtt gtttgttaag ccaacacaaa
     9121 ctgcaccaat aatctcttga tcataataaa tagggacgaa atagcggaag ccgaacccta
     9181 acattccttc tttttcagag aaaaattctt gtcctccaat cacttttttc acatcttcat
     9241 gacttgagaa aggaagaccg atgtaacttg ggtctgcgtg agaatatcga atgccttcag
     9301 ggtccatgac aacgataaaa tctaaattcg atgcaatcat gatttcattt gttattcctt
     9361 gaatcgcatg tgtcgggtca ccagtctgca gctttttttg gatgagttta tcattggcaa
     9421 cgattttagc aatgctagag attttatctg acacactaga tcggttcatc gaaagaataa
     9481 aattcctgat caatatggca gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa
     9541 ggatgatcgt gttctggagc tttaatggtt ttttcttaaa aaaaggaaac ataggctacc
     9601 tcgaaagaat ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa attgtcaacg
     9661 ataaacacaa ccttcctata aaaaacacca ctgagtcggt aaaagaagct atacagaatt
     9721 ttcaaacatt ggtatgatag acgtagaaat ggaaggaggc gtgtatatga tagggggaat
     9781 cgcacttgag acgatttatt ggtatacatt gctgatttgt gcaggattgg cagtcctatt
     9841 gattattttt ggagatgtat tcgattttga tggcccgctt gatccgatgc ttattatccc
     9901 ttggttaact ttcacaagct tatttggttt tctaggtgag cgtttaacag atcaatcatc
     9961 tttattttta tttattgcta gtggaggcat atcgatagtg ttggtatttt tgcttaattt
    10021 ctatatattg atgccgatga aacatgccga atcaacgatg tcgtcttctg aaaaaactct
    10081 ggaaggacaa gtagctgttg tgatcacacc gatccctgta cgaggtatgg gagaaattca
    10141 gttgaaaagt gttaccggtt ccattagtcg tccagcttgt ttttacagcc cccaagaaca
    10201 gacagctgag cggggaacga aagtattgat cattgagatc aaggaaagaa tctgttatgt
    10261 catgccttat gaaagcatga taaaagctta aaggagcgtg tgttaaatgg aaataccagt
    10321 tgtttttatc gcgatcgcaa tcgcagcttt gattttactg atgttgctga tcgtctttgt
    10381 agcgaagtac cagacagcaa aaccggatga agcgttgatc atcagtggaa gttatttagg
    10441 aagcaaaaat gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg
    10501 cgcattcgtc ttgccagttt ttcaacgttc gaatcggatc agtctattat cgagtaaatt
    10561 ggacgtctcc acccctgaag tgtatacgga acaaggcgta ccggtgatgt gtgatggtac
    10621 ttcgatcatc aaaatcggtt catctgtaga agagattgct acagcagcag aacagttttt
    10681 aggcaagaca agagaagaac tggaaaatga agcaagagaa gtattagaag gacacttgcg
    10741 ctcgattctt ggttctatga ctgtagaaga aatctatcaa aatcgcgata agttcagtca
    10801 aagcgtacaa gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt
    10861 cactatcaaa gaggtcagag acaaaaacgg ttatcttgac tctttaggga aaccaagaat
    10921 cgcccaagtc aaaagagatg cagatatagc agaagcggaa gctctaaaag aaacgcgtat
    10981 caaaaaagct caagcagaaa aagaatcaca agcagctgaa ttacagcgac aaacagagat
    11041 cgcagaatca ttaaaagaaa aagaactgaa attagcaacc tacaaacaag aacaagatgt
    11101 tgctaaagca aaagcagacc aagcctacaa tttagaaagt gcccgagcac aacaacaagt
    11161 aatcgaacag gaaatgcaga ttaaaatcgt agaacgccaa aaacaaatcg aactggaaga
    11221 aaaagaaatc acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc
    11281 cgatcggtac gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc
    11341 cgaagcagaa cgattccgtg tggaagcact ggcagaagcg gaagccaata agacacgttt
    11401 agctggacaa gcagaagccg aagcagcact agcaaaagga aaagcagaag ccgaagcaaa
    11461 acaaaagatc gccaatgcct tcaaggaata tggcgaagca gctgtcttga gtatggttat
    11521 cgatatgcta ccgcaattga tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat
    11581 ctctgttgtc gatacaggaa gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa
    11641 ttatgccaca aatttgcttt cttctactca agaaacacta aaagcgacaa caggacttga
    11701 cttgaaggaa atcgtagaga atttgtcgac acgtgggact actagccaag taaatatgta
    11761 tgaagcgcca aaagaggcag aacaaaactc gatgaaggaa gaataaagta gtacacttca
    11821 ccgttcaatc aaaaatggag gaagactttt taaagttctt ctctccattt ttttatttta
    11881 ttgcaaaaga aaattagtcc tcgctatttt tcttttgttt ttggatctta gcttctatct
    11941 cttgtaaatt tgttcttagc aatcgttctt tcttttggag ttcgaccaaa tcttctgata
    12001 gttcttcctt cttttctgtt tcaggaggac gaagaaagaa attgataccg attgcaatga
    12061 atgtccctat aaaagtatct agtacacgtt ggatagcata caaagcggat tcgccttgag
    12121 ggatgctcaa cgcaatcagc aacaacgtag ctattgcgga aatgataccg gaattattgt
    12181 taataccatc tgacacgacg atcacaataa taactagaat aggaagcaag atcaattcta
    12241 ccaaaaaatc attgtgaaaa tagcttttga caaaaaaata aatgatagct aatgtaccac
    12301 ctaatgtatt gccgatgatc ctagactttc caaaagaaac ggttgtagtc aagtcctgac
    12361 gcaaagaaaa aacagcagat aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa
    12421 aaaacaagat acataaaaaa acagctaagg cacttttgat cgtacgcata cctagtcgaa
    12481 aacggccaaa ttgcatagag attctccttt ttggtctttt tactatcata cctaaacttt
    12541 tgtctgatag ctagtttttc attattcaag tataattaaa ataaaaaagg gggaagtaaa
    12601 tgcgtatagg aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg atggtcacag
    12661 cgggtagctt atcgctgttg tattggccag ctgcagggat cattgcatta ttaagtgtag
    12721 gaaataccaa gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag
    12781 ctacggcaac agcactgttc agtttttttg ctgttgggta tactattttt ggcttcagtc
    12841 tttttttatt cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag
    12901 taaattcggt tttggtcact cattatttag tggaggaaaa catgtcttgg cagctgatcg
    12961 gcaatgaagc gggattaatg gcgattggat tagtatttgc tttgcttgca aatgtttaca
    13021 tgccagatcg tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa
    13081 atattttgag acaaatggca gaatttcttt tatcagaaaa taaagaagat gtgcagatca
    13141 aatgcgaaca tttactgaca ttcattcgcg aaagccaaga agatgccaga gaacatcaag
    13201 aaaactactg gctcagacag ccgctatact acgaaactta cttctctatg agaagagcac
    13261 aggccaatgt gattaaagac atgctggaaa atctagagag aatccagcaa ccagcttatt
    13321 atggaaaaca tatctatggt ttattgattt atactgcaga aacattttca gagtcaaatg
    13381 atggcagaca aattttaaca cgtattgaag aagtttatgt attgtatcgg caaatgcctc
    13441 tgccgactag tcgccctgaa ttcgaggatc gagcagaatt gttccagttt ttgcagtctt
    13501 ttaaaagttt tattgaaatc aaggcagaat tctcccaaca aaaaatccaa aaatagaggt
    13561 tgtgaattca gctaatattc aggtcaaaaa gctgatttca caacctcttc tttaaacggt
    13621 gttctcctgt caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt
    13681 tttgaaattg ctccttgtga ctcggagctg aacggtaggc tcacaacctc tttcttaaac
    13741 ggtgttcaaa agctttcctc tgcggtaata agtccagcca aacgaaaaat aggaggagct
    13801 atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa ccttttaaaa
    13861 ttacatattt ttccatagaa cttgcatttg atccaagatt tcgatgacat cttttgtcaa
    13921 gaagagagag cgattgggca agtcaccttc aatcatgtgt accatattct ttatttcgta
    13981 attcattgct tcagatgtat tcccgctttc gatgaattcc tttgtaccat ctgtaaataa
    14041 gatctccgca cgatcagcac gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc
    14101 aacgataccg acttttggca tttttgcctg aaaagtcaag ctgacagcag caagttcatt
    14161 ttctttgtta cgcaagatag tgacggattg ttcgtccacg cctgtttcaa aaggaaccat
    14221 tgtagaggca actacttcag gttgagagct caaaaagaaa cgagcgaaag atacagcata
    14281 cgtaccgata tccaacagag ctcctccagc taattctgga ttgaagaacc ggtttttagg
    14341 gtctggttct ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc
    14401 tgtatccatt attgagcgta actgctgata aagtggcatg ttgaagattg tcatcgcttc
    14461 tgctaagatc aaattctttt cttctgctaa gcgcatagcg tcagccagtt cttttttatt
    14521 catcgtgatg gctttttcac atagaacgtg tttgcctgct tccaatgctg ccaagatatg
    14581 gtcgatatgc tgtctatttg gtacagcaat gtacacgatg tcgatttcag catcgtctaa
    14641 catttcttga tagcttccgt aagcttttgg aatattgtaa cggtgagcga agttttccgc
    14701 tttttctgaa gttctggaag caactgctgt tagttcgctc gtctcttgat tgaagctttc
    14761 ggcaaactca tgtgcgatgg taccaagacc aacgattccc cattgatatt ttttcatctg
    14821 aaaacgctcc ttcatagttg gattgagttt cttgtctgtc tattattata acaaaaatca
    14881 cgaagaataa agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag
    14941 gtgctttttg atcaaaactt aagtagaata gttaaaagga ggggataaac gtgcatcaag
    15001 tatatattga tcttattatg agtgtattat tagaacagtt tgaaactgaa aaaaattttt
    15061 tagatgaata cttgctgatc agtccagaac aatggcaaaa atggaaagat ggcgagaaaa
    15121 atctgccatc tgaagcgatg cagaaagtaa aaaatctttt cactgattat gaatggatgc
    15181 taacgcaaaa gattttgcgt caaacgatta tttttcccga aaaacgaaac gttgccgttg
    15241 cagagtacaa gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa
    15301 aagtcgagct gatccctttg aaaaataatc agcaggctgg tagatacctt gacttgaaag
    15361 taagtattca atatgatgaa tggggctatg atgatatttt gaattttcgt ctgccagcag
    15421 atgttcaaaa acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc
    15481 tggaagaaac ttacgttcat gaggcgaatg gggaacgtga agaacaacga aaggattttt
    15541 caagtgaata aatttttagg atttggagca gttgtgatta tgatcgcagc aatgggctat
    15601 gttattttta gcgatgatga ttcgcatgtt caagccggca atcaagcagt cgaacgaaca
    15661 agcaccaaaa caactgctag taaagaaaag aacagtcaaa caaccagcag caaggtaaag
    15721 gacaaagaaa caaaagatcg tacgtccaaa caattgccag atgttaaaac agacgattgg
    15781 aagctagttt tggttggtcc agatcacaaa ttagaaaaag aaattgatga gtctactcaa
    15841 ttggcatcat taagtaacgg atacttgatc gacaaacgca ttgctccaga atatgaagca
    15901 tttgccaagg ctgctgaagc agctggattt ccattagtga tggtctcagc ttaccggtct
    15961 gtatcatccc aacaacaagt tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt
    16021 gtatcagaag aggaggcgac aaagattact aaagaaacga ttacggagcc aggatacagc
    16081 gaacaccaca caggtctggc agtcgatgtc gtcgatcaaa actggtacaa cagttatcct
    16141 tctactgtat tggatgcaag ttacggagat cagcctggag ctaaatggat cgcagataat
    16201 gctgcgcagt atggatttat cgttcgttat ccaaaaggac acgaagatat aactaagatc
    16261 acatatgaac cttggcactt acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa
    16321 caccaattga cgctagaaga atatctgaat gaattaaatg aaaagtagga attgatcatg
    16381 gcgaaaaaaa agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc agtggttgcc
    16441 ggtgtagcag tgatactctt agcctttgtt ttttccttga agaatctgtc ttctcctttt
    16501 actgataatc agcaagaaaa tgaagcgcaa tcccatcagc aatttattga acggcttgtt
    16561 ccgcatgccc aagaactaca agatggttat ggagtgcttc ctagtatcat tttaggacaa
    16621 gctatattag aatcaaattg gggaaaaagt accttggcta gcaaatacaa taatcttttc
    16681 gggatcaagg cttatgggga tcagaagaaa gtaagcctgg aaacaaagga atttgttaac
    16741 gaagaatgga taactatcca aggagacttc aaagtgtatg attcatggga acagtccatg
    16801 gatgaccata cacaactgtt cgtccaagga gtagattgga atcctgcgct gtatgaaaaa
    16861 gtgattacgg ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg
    16921 acagaccccg gttatgcgca aaaaatcatc caggtcatcg agacgtatca attgaatcag
    16981 tatgaccgga gatagcaaag gagaagtggc tgatggcaga acaatttacg ccagaaatca
    17041 tgacggaatt gagaaaagat atcgtaagag taccggaaat cattttagaa gcaagcggga
    17101 tcaagatatt tggaaaattg atccgttcga tcatctttac tacggatatt gcgatcatac
    17161 gtaatacaaa tgcgaatgca gtaattgctg tgtatccttt tacacctcat ccagcgatta
    17221 ctaaaagcat tattgaggca gctgacattc cagttttctc cggtgtaggt ggtggtttga
    17281 cacaaggtgc tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt tcgttagggg
    17341 tcgttttgaa cggtcctacg cctttagaga cagtaaaaga agtttgtcga gtagttgata
    17401 ttccagttat cagtacggta acgtcgaaat atacagaaat agatgaaaag ttagcagctg
    17461 gtatttcgat tatcaatatc agtgcaggta aagaaactgc agaagcagta cgccattttc
    17521 gtaaaaaata tcctgaactt cctatcattg cgacaggtgg tccaacggaa gaaagtattg
    17581 ctgagaccat tcaagcagga gcaaatgcca tcacatatac gccaccaagt agcggagagc
    17641 tttttagtcg taaaatggaa aaatatcggg aaactgagaa aaagaatcat gaataacagg
    17701 tgagaatcaa aagacgagga agagcagata gtggctcttc ctcgtctttt gtactccagt
    17761 actatttgtt cagaattcaa aaaaaagaga aatttttttg tttttttacc tgtaatgtac
    17821 ataagaagag ttaaaacctt ttgttattat tagcattcta acaaaaaaat gtcttgattt
    17881 attgaagcgc tttctataga atgaaaaacg aataaaaact aaaaaaggca cccgacaatt
    17941 tagtagcagc taaattgtcg gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc
    18001 atagtcaatg cttgtgcatc gacgtgtata ttgtacctaa tttcatacag aattactagt
    18061 tatttttctg ttttttaggg aagagcgcac acgaagaagc atgcttattt agctatgtca
    18121 aggatattgg gcagttgtct gatatccttt tttctttgcg taattatcct cccatcattg
    18181 aagcaagaag tgtttttagc gatgaaagaa gaatcagcac tatctaattt ttcttgaaag
    18241 tattgagttg ttcatcctag gcagaacgat aaggcaagtc ttggttttta ttccccttga
    18301 aaaagatcgt ttaataaata tgttctgtat aaaaatacag tatattgaaa aataacaatg
    18361 gaagtagagg tgcagagaat gaagaaaaga atgagaatca tcttagcttt aagtgttatg
    18421 ttattcttct tgacggcttg tgggcaagaa gatgacaaaa aaatcacgct gaacatggct
    18481 tggtggggtt cgcaagtccg acacgatgca acagtaaaag taatcgagct ttatgaagaa
    18541 aaaaatcccc atgtgacgat cgattatgaa ttttatgatt atgaaggata ttacacaaaa
    18601 ttaggtacac tagcagcatc agatgatatc tgggacattt tccaactgaa caatcaattt
    18661 cctcaataca tcaatcagat tgaaccatta gatgaatatg tggagcaagg gataattgat
    18721 atttctaatg cagatgaatc ctatttagca acgacgactt acgacgggca attagttgga
    18781 ctgtctaacg gggtgaattc gtttgcgatt gcttacaata aaaatatatt tgaacaatta
    18841 aatatccctg aaccagcttc aaattggaca tgggatgaat ttgaacaaat cagctatcaa
    18901 atcactgaag aattaggaat ctatgctatt tcaagattcg aagattttat agctggctgt
    18961 attattcaaa taccacaagt tgagaaagga ttgaattttt ataatcggga agatctatct
    19021 aacagcttgg gatttgaaaa tccagactat ctaacagatt ttttccagct gcgtaaaaat
    19081 ctggtagaca aaggggccca tccagaacca ggtggtggag ctgcaagtac gacggatctg
    19141 tcttttcagg cggttaggga tagagaagcg gcaatggtgt ttctatcaag taaccagttg
    19201 acagaaatat tgtcaggggc tcctgaagat atggagattc agttgattaa tccacctcgc
    19261 agaaaagcag atggagaatc tggtatccct ttacgttctt cgcaaatgct gagtatggca
    19321 aaaagctcag cccataaaga agaagctgct aagtttattg atttctttca aaacagtgaa
    19381 gaagccaatg aaattttaaa aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa
    19441 cagctcgctc gtgcagatga tgcaactttg caagcgacat ttgattattt agaaatgatt
    19501 tccgatatgg acaacggcga agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa
    19561 gaccaataca atcttttagt tgaaaaagtc atttatgaag aagtaacagc agaaggaagg
    19621 catgaaaaaa gaaaaactaa aacggttctt ggatcaagac aatgtggtcg gttacgtatt
    19681 tgcttctcca ttcatcctag gatttctatt gataacagtg ataccgatga tcttgtcact
    19741 ttattattcg ttcaccgatt atcgtttggg aaatccagtc tcatggatcg gcttggataa
    19801 ttatataaag atgctgagtg atgcccgttt tttgaactcc atccgagtca cttttctcta
    19861 tgtatttgca tctgtacctg tgaaactgat ctttggtcta ttgattgctt atcttttaac
    19921 gaggaatatc aaaggaacga ctttctttcg ttctttgtat tatgtgccta gtttgatcgg
    19981 tggcagtatt tctatcgctc tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa
    20041 tattttcctg gaacgattag gcgcacctgc gctttcctgg tttagcgatc aaaaactggt
    20101 catgatccct cttgttttga tgtctgcttg gcaattcggt tcggcaatga tcatctttgc
    20161 tgcaggtatc aaagagatcc caggctcgta ttatgaagcg gcagagatcg atggagcgaa
    20221 caaatggcag acgttttaca agatcacgtt acctcttctt tcgccgatca ttttgtataa
    20281 cttggttatg cagacaatcg cggcgtttat gacttttacg caggcattta tcattaccca
    20341 aggtggtccg aatgatgcca cgaactttta tgctctttat gtgtataacc aagctttttc
    20401 ttttaattct atggggtatg ctagtgctat ttcgtggatc atgctgttga ttatgggagc
    20461 tgtcaccttt ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg
    20521 aagaaatgaa taatacagta acgaaatggt ttcaatacgg ttttattttg ctattaggtc
    20581 tagtcatgat ttatccggtc ttatggatga tcgcaggttc ttttaaaacg gatcaagaga
    20641 ttatgagtgg ttcgctgaat ttgattccag agacttttca atgggaaaat tacgcaaaag
    20701 gatgggcggg gtttgctgga atttcatttt ttactttctt taggaattcg tttgtcatct
    20761 ctgcagtttc gacgataggg acagttctga gctcgacttg tattgcttac gcattatcga
    20821 gaatcgattt caaaggaaaa agattctggt tcgtcgtcat gttagtaacg atgatgattc
    20881 ctgcacaagt gattttgatt ccgcagttta ttatttacaa tcggctgaat ttggttggga
    20941 cgtatgtgcc gctgatttta ccacattttt ttggacaagc atttttcatt taccagatca
    21001 tgcaattcat ggtcagcatc cccaaagagt tagatgaatc agcgattatt gacggatgca
    21061 gcaagtatag tgtctttacc aaaattattt ttcctttgct gaaaccttcg atcatcacga
    21121 cgatcatcat ccagttttat tggaaatggg acgacttcat gggaccattg atttatttaa
    21181 acaagccgag atcttacacg gtatcgattg ccatcaaatt atttgcagac gctggatcta
    21241 gtacgactta cgcgagtatg tttgcgatgt ctacgttgtc gctgctaccg gtttttctga
    21301 ttttcttatt cttcaataaa tatcttgtcc aggggatcag tacaagcggt ctgaagggct
    21361 gaatatgaaa aagccaatag aagagatgtt tctttacaag ctcaactact atgtctgtcg
    21421 gattttttta accagtctta tcagtaatgc tcttttcgct ttagttctag gatgtttgtt
    21481 cactttagga ttagtgctgt ctgctcgtgt tgtctatctg atttattttt tgatgatgta
    21541 tccttcttat tatctatctg tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg
    21601 gaaaatcaaa cggatgtgtt tcatcattac gttgtttcag ctgtttgtca ttatttttgc
    21661 agttgattgg tatttattag tgggaaagat tggagcttgg tatctgattc cttttctatt
    21721 gtgtctgttg ctttttgtat cccatagttt ttttagtacg ctaattgata tctgcaaaga
    21781 agataaagca gatagtcttg tttatttgat caaaaggaat atctggctgt tgtcaaaaaa
    21841 cgtcaagggg tttctgtggt atttatgcct tttgtatgga ctgttctggt gtatacagcc
    21901 tgatttttta ctcactgcat tactgtatcc agggctgcca tttgtcacag tattttgctt
    21961 aaaagaaaag gagaatgact ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg
    22021 agagcgcgtt ttttttatga ggcaattgcc tctacgtatc atcaaacttg tgaactagtc
    22081 ggtttttgtg atgtatctca aacaaggatg gattatgcaa atcggatact tgtgaacaag
    22141 tatggacaga aaaaagtaaa gacatgtatg ccatcacaat ttgagcaact actggatgac
    22201 gtacggccgg ctgaagtcat tattacgtca gtagatcgaa cgcatcatga ttatatcatt
    22261 cgtgcaatga aaaaaggata tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa
    22321 aaagctcaag aaataataac ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt
    22381 aattatcgtt atgcaccgta tcacacgaag ataaaggaac tgatagaaag tgatgtgatt
    22441 ggtgatgtgt tttctatcca cttcgaatgg ctgttaaata cgcaacacgg ggcagattat
    22501 ttcagaagat ggcatcgaaa taagcacaat agcggcgggc ttcttgttca taaatctacc
    22561 catcattttg atctagtgaa tttttggttg aacagccgtc cccaaaaggt atttgcattc
    22621 ggtggattaa acttttatgg gatgcataat gcggagaaac gcggagtaga aatattttac
    22681 agtcgatgct atggaagcga actagctaaa gctgatccat ttgctataga cctgtcagcc
    22741 aatgaggaat tgcgagaaat gtatttagaa gcagaaaaag acagtggata tattcgtgac
    22801 cgcagtgtat tttccgatga tatctctatt gaagatacac taggcgtaac ggtcaaatat
    22861 gaaactggtg cgattttgac gtattcgcta aacgcgtata tgccgtggga aggattcaat
    22921 gtttctttca atggcagcaa aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc
    22981 aatgcaggtg gagataaaaa ggatgaagga gcagccgttc ttcagtccat ccatgtctat
    23041 ccgatgttcg gtgagccata tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga
    23101 ggagatccgc gtttattaga tgatctattt ggtgaagaaa aagaagaaga tccgtggaat
    23161 cgggcagcaa ctcacgtaga tggtattttg tcgattttga caggaatcgc agcaaatcat
    23221 tctattgctt caggaaaagc agtagtgatt gacgagcttg tcagcttaaa agaataagaa
    23281 tagatttacg gaaattactt cccgtagttt ataaaatctt gccttattgc cgataggctg
    23341 tcttttgaac actatttatt cggaaaaagg ggctatgaca tgctttttgt catgccttct
    23401 ttttatgtgg gctgcttttt tagataggag aaaataaaaa taactattta aacccgaaca
    23461 aaaaggaata atgcattaaa aatcattaga ttttacaaaa aaataagtaa accacgttgt
    23521 cagtttcaga aagtagtgtt agactgtttt caatatttga aacaacattt tttattcaaa
    23581 tatggcaaac tgccttcacg tgcaaaacag ttgcaagaaa atgaatttac actattatta
    23641 gttttacata cctacttata tatcgtcata atatggatga cagtttctag ccagtaccgt
    23701 aaatgctgga ctataagtaa aagattggct atttcaccct tcttttctta tgataaagtg
    23761 gagggtgttt ttctttaaca gaaacatagt aggtttttgc acaaaagcgg ttatttcggt
    23821 attaataaat gataaaggag caaataaagt gaaagaatta gttgaacgta ttcaaaaaga
    23881 cggacgtata ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc accaagtaga
    23941 ccctgtattg atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat
    24001 cacgaaagtt gtgaccattg aagcttctgg gatcgcacca gctttatttg ctgcaagaga
    24061 attagatgtt ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt
    24121 attgacagct tctgtttatt cttttacgaa acaagtgaca agtacgattt ctatttctcg
    24181 taaattttta tcttcagaag acaaagtatt gattatcgac gatttcttgg caaacggaca
    24241 agcggcaaaa ggattgatcg aattatgcca gcaagctgga gcgcaagtag aaggaatcgg
    24301 gatcgtcatc gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa tgggattgag
    24361 agtcgtttct cttgcgcgta ttgcttcctt gacgaatgga caagttgagt ttcttgagga
    24421 ggacgcataa tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt agggctgcag
    24481 catctgctag cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg tacaggacta
    24541 ggctttaatc aagaacagat gacttatttg atttcaattg atatctttat gtgtggatta
    24601 gcgacattat tgcaattgac agttaaccga tttttcggaa ttggcttgcc tgtcgtttta
    24661 ggatgtgcga tccaagcagt agctccactg atcttgatcg gaaccaatga aggagtagga
    24721 gctatctacg gatctattat cgcttctggg atctttgttg tgttagtttc tggtgtcttt
    24781 tctaaaatta aaaaactatt ccctcctata gtaacaggaa cggttatcac cgttattggt
    24841 ttgactttga ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac agcagctgat
    24901 tttggtgaca aaacaaatct gctacttgca tttgtgacga ttctcctaat tgtaggcgtg
    24961 caaatgctgg caaaagggtt cattcgttca atcgcggtat tgattggttt agtcggagga
    25021 agcatcttag ctgctttcat gggaatgatt gatctgagcg cgattggaga tgcgccgttg
    25081 ttccatgttc cacaaccctt ttattttggt aaaccaactt tcgacgtatg gtcgatttta
    25141 ctaatgatca tcatttccat ggtcagcatg gtagaatcaa caggtgtata ttttgcactt
    25201 ggtgacatta ctgggaaaaa tgttggagaa gaagaattga aaaagggata tcgtgcagaa
    25261 ggacttgccg ttatcttagg cggtattttc aatacattcc cttatactgg cttttctcaa
    25321 aatgtcggat tggttcaact ttcgggaatc aaaacaagaa aacccatcta tttctctgca
    25381 ttcttcttga ttgttttagg attattccct aaaatcggtg cgttagcaca aatcatccct
    25441 gagccggtac ttggtggagg aatgttagtc atgttcggga tggtggcagt ccaaggaatc
    25501 cgtatgctgt tggaagttga tttcagcaat gataaaaatc tattgattgt tgctgtttct
    25561 gttggatttg ggctgggttt caatgttacg ccgacattat tcagtcaaat gccgcaaaca
    25621 ttgcaaatgt tcactggaaa tggcatcgta atgagtagca ttacggctat catattgaat
    25681 ttgatcttta atggattgaa aaaagatgaa gaaatattgg aagaagcctt caagaagtaa
    25741 tagagcaaaa gaaaggttgt gaaatcagcg ttttgacctg attgttagag aaacaaacgg
    25801 aaaaatagtt cctcgtattt tgccgtttgt ttgagctaat accgagggtc agctgattga
    25861 acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct ttttttcatc
    25921 tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat
    25981 caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc
    26041 aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca
    26101 aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc
    26161 agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc
    26221 aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt
    26281 tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt
    26341 ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa
    26401 tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt
    26461 ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta
    26521 aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga
    26581 tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt
    26641 gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc
    26701 tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag
    26761 aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga
    26821 ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc
    26881 cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc
    26941 cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa
    27001 gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag
    27061 catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt
    27121 tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg
    27181 ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg
    27241 cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg
    27301 ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag
    27361 cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag
    27421 aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg
    27481 aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac
    27541 aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa
    27601 cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac
    27661 gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc
    27721 tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag
    27781 atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa
    27841 aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag
    27901 aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct
    27961 atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca
    28021 ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc
    28081 atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga
    28141 acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag
    28201 tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga
    28261 tccgtccaca agaaatctct actcaagttc tccctcggga tctacatgct gaatatttct
    28321 cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc
    28381 aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg
    28441 ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg
    28501 tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt
    28561 ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt
    28621 taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga
    28681 atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata
    28741 agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg
    28801 acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat
    28861 ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga
    28921 tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga
    28981 atagcaaaaa taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat
    29041 tatattttct tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc
    29101 tctttacata caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat
    29161 gctggttatg gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat
    29221 tgaataagat ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga
    29281 ttgaaagttc gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac
    29341 agcgcttggt tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata
    29401 aataaacgtc tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta
    29461 attctgataa aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg
    29521 aacccattat ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac
    29581 gcttgttgat ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat
    29641 accttaaatg tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga
    29701 aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat
    29761 ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga
    29821 gaacttgatt tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga
    29881 ccagataagt ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct
    29941 gactcaaaga ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc
    30001 aaaaaaagta caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt
    30061 ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta
    30121 ctataaggat gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca
    30181 acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat
    30241 tttgaccgaa ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg
    30301 ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg
    30361 ggatgaacgt acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt
    30421 gatcccagtc tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg
    30481 agtatgatgt atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa
    30541 ggggaggcag taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga
    30601 ataggaaaat actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt
    30661 gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc
    30721 aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg
    30781 tgacgcagat atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg
    30841 gcatgatgag tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta
    30901 tggtgactat ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat
    30961 tcgttttgct gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac
    31021 agaagcagga cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa
    31081 aactgtacca ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga
    31141 aataatctat cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact
    31201 tcaagaacta aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag
    31261 cgtggaaaac attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca
    31321 tccagaacgt gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc
    31381 cattttaaaa aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc
    31441 tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg
    31501 aatgatcgaa gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc
    31561 tgttatgtgg agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc
    31621 tactagtgga cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg
    31681 tgatggacta gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc
    31741 ttatgaagga gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc
    31801 tcgtccaatt gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa
    31861 atatttacta gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc
    31921 gactgtaggc ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc
    31981 aatgtgcgta ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg
    32041 taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt
    32101 tgcctcagaa gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga
    32161 tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga
    32221 ttctctaata ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat
    32281 ggcttcaaaa gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac
    32341 tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt
    32401 taaatcagga gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt
    32461 cactatcggt aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt
    32521 agcaaatctg ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa
    32581 agaaccagct cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga
    32641 acctggacaa gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat
    32701 ctacgaaact tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc
    32761 tgctgtgatg agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc
    32821 gcgctatctg tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag
    32881 aaatattatt gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc
    32941 tcctgataaa ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc
    33001 gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga
    33061 tggtcaagca atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa
    33121 acatatcact actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac
    33181 gtttgcagat tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt
    33241 gatccgaaac tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat
    33301 ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact
    33361 ggcagaatca gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc
    33421 tcaattattt gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac
    33481 acgattcgaa gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc
    33541 gataatcgaa atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa
    33601 gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg
    33661 atcttcggtg tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt
    33721 cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag
    33781 gggcatcgag atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag
    33841 ttaacaggag aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg
    33901 gacaatattc agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat
    33961 ggtaacttga tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc
    34021 cattctaact ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat
    34081 tttttaaata aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta
    34141 atgacagaaa cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc
    34201 ggacaaatga aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt
    34261 ggggcacgtt ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga
    34321 atccagattg attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc
    34381 tattttgcca gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat
    34441 atgggacgga atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct
    34501 aattcttctt tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc
    34561 ggattagtaa aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga
    34621 gagcaaggtg ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg
    34681 attatggtcg atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg
    34741 aaagaagcag gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca
    34801 tgtttttacg ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt
    34861 gatgaaatca aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg
    34921 atcgaatcga tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc
    34981 aacggagatt atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa
    35041 gaaaaagtat cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa
    35101 tgcatacgca aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa
    35161 gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg
    35221 ttgttttgat ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg
    35281 cgtggggacg aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga
    35341 ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt
    35401 agactactta gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt
    35461 agcagatgga tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc
    35521 tggtatgtac tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa
    35581 atctcaatta ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc
    35641 tagcgggatt cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca
    35701 gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga
    35761 gccaacgaaa atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg
    35821 agctgcacat atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc
    35881 tatggctgca caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca
    35941 aaaatatggt gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat
    36001 ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg
    36061 cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat
    36121 aggagaagaa cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg
    36181 ctagccgatt atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat
    36241 cagcctgaag catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt
    36301 tcacctaaag aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa
    36361 gaaaaaaaga tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg
    36421 ctgaaaaatt atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct
    36481 ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt
    36541 cattatatcg accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt
    36601 gaacaggagg acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc
    36661 tatccagaag tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat
    36721 tgatcagtgt atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag
    36781 gagtagaaat catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa
    36841 cgctttcgat cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac
    36901 ttcatcctaa aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg
    36961 caatggaaac ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca
    37021 aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg
    37081 gtggtcctag tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg
    37141 atccatctga ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt
    37201 ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga
    37261 tcgctcaata cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt
    37321 atgaacgaaa acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc
    37381 agtccgctct tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac
    37441 tttcttataa taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg
    37501 agccaacggt agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga
    37561 ttttagaagc gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca
    37621 tcgttttgaa tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag
    37681 aaatcatcat tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa
    37741 acatccgatt actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg
    37801 tctcagtctt aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag
    37861 agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg
    37921 cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga
    37981 cagtaggaat cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac
    38041 aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg
    38101 attcggttga atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca
    38161 tcaaagacca agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg
    38221 atgttcggca ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg
    38281 caggtggtcg tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg
    38341 tgtattgtgc gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca
    38401 gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta
    38461 tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat
    38521 tgaaaatctt cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta
    38581 aacaattaat ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg
    38641 aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct
    38701 tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag
    38761 aacaaatgtt gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt
    38821 ttttggcagg agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga
    38881 tggtcatttc gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg
    38941 gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg
    39001 aagaagtttt aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg
    39061 ttttatatgc gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc
    39121 gtttcggtga tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag
    39181 taatcgatga tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca
    39241 atgtaggcgt tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc
    39301 taccagattt tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg
    39361 gcaaactagt tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca
    39421 aagaagcaca gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt
    39481 atcgtacaga tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct
    39541 gcttccattt ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt
    39601 ttcataaaga gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa
    39661 aaaattcaat taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt
    39721 atcaacaatg cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat
    39781 ttttttgacc atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct
    39841 ctagcaaaaa catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt
    39901 ccaggtgtga tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg
    39961 acccgtctga aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta
    40021 gaaccagagg aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat
    40081 tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa
    40141 ccattagtag ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg
    40201 attcatgtca atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat
    40261 tcccgattgc atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa
    40321 ggtgtattta tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca
    40381 gctaaatatc aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc
    40441 aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct
    40501 tcagaaatta atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt
    40561 ttaccttaaa gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac
    40621 ttgaaagctg ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta
    40681 tcattgtacg gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata
    40741 ttcttttgga gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt
    40801 ataaccaatt tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt
    40861 tgctaattcg attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga
    40921 aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga
    40981 catatctgga tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg
    41041 atatttgttc cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac
    41101 tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca
    41161 atgaaccatt tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt
    41221 caagacaaat aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg
    41281 ttcgatgtac ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa
    41341 gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat
    41401 ttgttcttta agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg
    41461 taattcgctg attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc
    41521 cttttctaat tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc
    41581 ttcaagagaa tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac
    41641 ttgttgattc tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa
    41701 gtctgtactg acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg
    41761 attgaaagat tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc
    41821 gttcaagaaa tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt
    41881 ttgctgcaaa tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt
    41941 aatcggttta agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa
    42001 atcgtcgaat ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt
    42061 tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg
    42121 taagtcgata attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat
    42181 tggataataa gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc
    42241 gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag
    42301 ggatcatcag tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt
    42361 gatcatttcc gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac
    42421 tgaaatctga atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag
    42481 ggatgccaat cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg
    42541 taatacgaat aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa
    42601 tcaatggctg caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga
    42661 tcttatagtc aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat
    42721 ggctaatttc tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa
    42781 agccggaaag tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag
    42841 aaataatggc atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac
    42901 gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta
    42961 gatcttccgc cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca
    43021 gggttaaacg aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat
    43081 tgatcggttc ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa
    43141 tggtactaag aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg
    43201 tatttttttt gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca
    43261 gcagttgagt gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt
    43321 ttgccaagat attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta
    43381 gcgatacatc taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa
    43441 tatcattcct aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc
    43501 cagaaatcaa gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat
    43561 ctgctttttg gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca
    43621 gtatgctcag tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa
    43681 aaatctgttt tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt
    43741 gggatgttag ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt
    43801 aatgtttttc ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag
    43861 ccgacatcag gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc
    43921 atatcttctt gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt
    43981 tcttttatcg cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg
    44041 atttcggata aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga
    44101 ataaaatcac cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca
    44161 tcttttttta gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg
    44221 gtcggtgtgg agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa
    44281 tttgtgaagt gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg
    44341 ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg
    44401 acagggatcc ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa
    44461 ggagtaaaaa tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc
    44521 cgttcctgat tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca
    44581 gtgataattg attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa
    44641 ccaacgacta gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa
    44701 gcgattacaa caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg
    44761 atttgttagt actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga
    44821 aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa
    44881 gtaaatccca taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca
    44941 attgaaatgg gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta
    45001 ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta
    45061 cggggtatgt aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga
    45121 acaacatggt ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag
    45181 ataaagtaga aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac
    45241 ttgtgattgc ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag
    45301 cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt
    45361 acgcgacttt tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact
    45421 taggattgaa agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg
    45481 acaacaacgc actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca
    45541 acggtcagtt ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct
    45601 gggatggttc tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc
    45661 aaacattgga tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat
    45721 taaaaggcgt ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg
    45781 cgacagttgc tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag
    45841 acacacgtat cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag
    45901 aggttccggt caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt
    45961 tagcaaatac agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt
    46021 attataaaga atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta
    46081 agtcagagag aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa
    46141 aaaattttct ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat
    46201 ccatgcattg tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg
    46261 cttgtatcca tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt
    46321 taaagatttg aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact
    46381 aagtccttat ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg
    46441 cgtgattgat tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt
    46501 gtctatcgac ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga
    46561 aatagctaaa gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc
    46621 attgaatgaa gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg
    46681 tgtaacctcc atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat
    46741 caccatttta cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga
    46801 gcatattatt cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc
    46861 ggatattgga gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca
    46921 cgagcgaatc ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat
    46981 agctggactg atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta
    47041 tggaagcaag atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt
    47101 tccttcagcc attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact
    47161 gaatctgctg atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca
    47221 aaaaggagta ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat
    47281 gcgtataaaa acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca
    47341 aaaagtcgtt ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc
    47401 aacccgtggg atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc
    47461 agcaaatggc aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg
    47521 tgatcgaatc tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc
    47581 agatcaggaa gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg
    47641 caaaaaatct tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc
    47701 tttttggtcg ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga
    47761 tgacggatgg tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc
    47821 atatattagt actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt
    47881 cggtagggtc agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc
    47941 aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat
    48001 ggcaaggttt ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat
    48061 tgctgatgtt tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc
    48121 cagctgtttt ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc
    48181 ttcacttctt gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat
    48241 ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct
    48301 tggtaaaggc ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt
    48361 atcaagggta tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt
    48421 tgacaaataa aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg
    48481 ctgaactatc agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag
    48541 tcatggcagc tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag
    48601 caggtacatc gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt
    48661 ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga
    48721 acaatggcat gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga
    48781 tcttgctttt agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga
    48841 acgccggtta ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta
    48901 aaagcgaaca ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat
    48961 tcgtataaat tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg
    49021 cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag
    49081 gaggataatg taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt
    49141 atcgtaaata gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa
    49201 atgccagtag tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta
    49261 gcggaaaatg gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt
    49321 atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag
    49381 gaatattcct ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc
    49441 gacatcgcgc atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat
    49501 ttgcctgaaa cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg
    49561 gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca
    49621 aaaatcaaga caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg
    49681 aaagcagctc gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca
    49741 aaatctgtcc gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag
    49801 gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct
    49861 gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa
    49921 cacaaaggat ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt
    49981 tcttatgctg gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa
    50041 aattcgattt ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa
    50101 aaagttgtcc tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt
    50161 gtttggctgg aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg
    50221 actgtgataa gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc
    50281 tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg
    50341 aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag
    50401 aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc
    50461 aactttttgg tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg
    50521 gggtactaac gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat
    50581 tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact
    50641 ggtatgagca aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat
    50701 acgctagcga gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg
    50761 aagaaatctt agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca
    50821 aaatcaaacg gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg
    50881 tgactggtgg cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag
    50941 aggggatagc tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag
    51001 gaacgcgttt atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag
    51061 aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta
    51121 caggaaaagt gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc
    51181 ggcttccaat tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag
    51241 tccttcctat acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg
    51301 ttttaggaaa aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac
    51361 tagctcaagc gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg
    51421 ctaatgtatt taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag
    51481 aaaaaagccg actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa
    51541 ttttaggtgg ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag
    51601 cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata
    51661 taaatggaat cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt
    51721 caaagatgaa aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa
    51781 agaatataac agcttggatt taaa
//
LOCUS       DACCIQ010000010        51119 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCIQ010000010 DACCIQ010000000
VERSION     DACCIQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 51119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.010"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            115..960
                     /locus_tag="IVM62_000593"
     CDS             115..960
                     /locus_tag="IVM62_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6844579.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1241..2461
                     /locus_tag="IVM62_000594"
     CDS             1241..2461
                     /locus_tag="IVM62_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP6844580.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2458..5178
                     /locus_tag="IVM62_000595"
     CDS             2458..5178
                     /locus_tag="IVM62_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6844581.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5162..6106
                     /locus_tag="IVM62_000596"
     CDS             5162..6106
                     /locus_tag="IVM62_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6844582.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6175..7185)
                     /locus_tag="IVM62_000597"
     CDS             complement(6175..7185)
                     /locus_tag="IVM62_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6844583.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7365..7709)
                     /locus_tag="IVM62_000598"
     CDS             complement(7365..7709)
                     /locus_tag="IVM62_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6844584.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7702..8136)
                     /locus_tag="IVM62_000599"
     CDS             complement(7702..8136)
                     /locus_tag="IVM62_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP6844585.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8274..9011
                     /locus_tag="IVM62_000600"
     CDS             8274..9011
                     /locus_tag="IVM62_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844586.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9004..10215
                     /locus_tag="IVM62_000601"
     CDS             9004..10215
                     /locus_tag="IVM62_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844587.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10422..11201
                     /locus_tag="IVM62_000602"
     CDS             10422..11201
                     /locus_tag="IVM62_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP6844588.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11271..11933
                     /gene="trmB"
                     /locus_tag="IVM62_000603"
     CDS             11271..11933
                     /gene="trmB"
                     /locus_tag="IVM62_000603"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP6844589.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12031..13785)
                     /gene="ade"
                     /locus_tag="IVM62_000604"
     CDS             complement(12031..13785)
                     /gene="ade"
                     /locus_tag="IVM62_000604"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP6844590.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13917..14846
                     /gene="cpaF"
                     /locus_tag="IVM62_000605"
     CDS             13917..14846
                     /gene="cpaF"
                     /locus_tag="IVM62_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP6844591.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14803..15852
                     /locus_tag="IVM62_000606"
     CDS             14803..15852
                     /locus_tag="IVM62_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP6844592.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15849..16127
                     /locus_tag="IVM62_000607"
     CDS             15849..16127
                     /locus_tag="IVM62_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP6844593.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16102..16572
                     /locus_tag="IVM62_000608"
     CDS             16102..16572
                     /locus_tag="IVM62_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP6844594.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16589..16891
                     /locus_tag="IVM62_000609"
     CDS             16589..16891
                     /locus_tag="IVM62_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844595.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16908..17279
                     /locus_tag="IVM62_000610"
     CDS             16908..17279
                     /locus_tag="IVM62_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP6844596.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17251..17610
                     /locus_tag="IVM62_000611"
     CDS             17251..17610
                     /locus_tag="IVM62_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844597.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17782..18789
                     /locus_tag="IVM62_000612"
     CDS             17782..18789
                     /locus_tag="IVM62_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6844598.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18814..19998
                     /locus_tag="IVM62_000613"
     CDS             18814..19998
                     /locus_tag="IVM62_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP6844599.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20435..22018)
                     /locus_tag="IVM62_000614"
     CDS             complement(20435..22018)
                     /locus_tag="IVM62_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6844600.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22596..22739)
                     /locus_tag="IVM62_000615"
     CDS             complement(22596..22739)
                     /locus_tag="IVM62_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP6844601.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22962..23441)
                     /locus_tag="IVM62_000616"
     CDS             complement(22962..23441)
                     /locus_tag="IVM62_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6844602.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23514..23798)
                     /locus_tag="IVM62_000617"
     CDS             complement(23514..23798)
                     /locus_tag="IVM62_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844603.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23876..24070)
                     /gene="ssrS"
                     /locus_tag="IVM62_000618"
     ncRNA           complement(23876..24070)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVM62_000618"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24142..25428)
                     /locus_tag="IVM62_000619"
     CDS             complement(24142..25428)
                     /locus_tag="IVM62_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP6844604.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25566..26237
                     /locus_tag="IVM62_000620"
     CDS             25566..26237
                     /locus_tag="IVM62_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP6844605.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26203..26691
                     /locus_tag="IVM62_000621"
     CDS             26203..26691
                     /locus_tag="IVM62_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP6844606.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26704..27651
                     /gene="prmA"
                     /locus_tag="IVM62_000622"
     CDS             26704..27651
                     /gene="prmA"
                     /locus_tag="IVM62_000622"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP6844607.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27652..28416
                     /locus_tag="IVM62_000623"
     CDS             27652..28416
                     /locus_tag="IVM62_000623"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP6844608.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28649..30862
                     /locus_tag="IVM62_000624"
     CDS             28649..30862
                     /locus_tag="IVM62_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP6844609.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30873..31319
                     /locus_tag="IVM62_000625"
     CDS             30873..31319
                     /locus_tag="IVM62_000625"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP6844610.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31490..32749
                     /locus_tag="IVM62_000626"
     CDS             31490..32749
                     /locus_tag="IVM62_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP6844611.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32838..33785)
                     /locus_tag="IVM62_000627"
     CDS             complement(32838..33785)
                     /locus_tag="IVM62_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6844612.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34179..35471
                     /locus_tag="IVM62_000628"
     CDS             34179..35471
                     /locus_tag="IVM62_000628"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP6844613.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35486..37255
                     /gene="aspS"
                     /locus_tag="IVM62_000629"
     CDS             35486..37255
                     /gene="aspS"
                     /locus_tag="IVM62_000629"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP6844614.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37364..38239
                     /locus_tag="IVM62_000630"
     CDS             37364..38239
                     /locus_tag="IVM62_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6844615.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38505..40388
                     /locus_tag="IVM62_000631"
     CDS             38505..40388
                     /locus_tag="IVM62_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6844616.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40463..40747)
                     /locus_tag="IVM62_000632"
     CDS             complement(40463..40747)
                     /locus_tag="IVM62_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6844617.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40737..41471)
                     /locus_tag="IVM62_000633"
     CDS             complement(40737..41471)
                     /locus_tag="IVM62_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP6844618.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41600..42223
                     /locus_tag="IVM62_000634"
     CDS             41600..42223
                     /locus_tag="IVM62_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP6844619.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42415..43536
                     /locus_tag="IVM62_000635"
     CDS             42415..43536
                     /locus_tag="IVM62_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP6844620.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43543..46671
                     /locus_tag="IVM62_000636"
     CDS             43543..46671
                     /locus_tag="IVM62_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP6844621.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46690..49884
                     /locus_tag="IVM62_000637"
     CDS             46690..49884
                     /locus_tag="IVM62_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6844622.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50030..50737)
                     /locus_tag="IVM62_000638"
     CDS             complement(50030..50737)
                     /locus_tag="IVM62_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6844623.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16727 a         8940 c        10618 g        14834 t
ORIGIN      
        1 tcctattctt aaaatgatgt ttgtacttac ttaaattagg tataattagt atatgttttg
       61 tgagaaattt agacttacaa taagcagtga atttataatc aacgtatagg agacatggat
      121 gaaatgaaac taattggtat agatttagac ggtactcttt tgaattctga ccagagcatt
      181 agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag actgttttcc attcatttgt
      241 tctggaagag aagtagaaga cattaataat attctcaaaa aatctggttt aaggatacca
      301 gcagtagggt taaatggttc tgttggattt gatggagaaa aaaagatatt tgagtcttcc
      361 ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc aaccaaaata
      421 tatacaaatc tcggaagtta tgaatctcag aattataaaa ataaaatgaa aaaaattttt
      481 cacgaggtag ggagtgaatt ttctatcaaa gagctaaact atgagcttga gtatgaaaaa
      541 tctatttgtt caatccctta taataatatt gaggaaatta tagaaaagga tgctatcagc
      601 atttacaaat tttttatttt tattcctaat cgtcaattga aagctaatat atttaaccat
      661 ttgaaaaaga tgctaaatgt gtctgttact gaatcggcac ctgtaaatat tgaaatcgta
      721 cctcaaaatg tatctaaagg tgtggtgttt gatcgtttgg aatcaatcta caatttaaag
      781 aaacctttga gaatcgcgat cggagatagt ttaaatgact tatctatgtt tgaaagtgca
      841 gatattagtt ttgcaatggg aaatagtcat caagaaatca tgaaaaaagc tgattatgtc
      901 actgtttcaa atgataaaga cggagtatct caagcatttg aaagaattct taccatataa
      961 actaattaag gtagtataga acatagaatt ctaaatacgg aaaaatggag cataaattcc
     1021 agtacaaata gtacttcttc agtgtcaaac gaagcgaata taacatagat atttaggatt
     1081 gtgacaaaaa tcatgtcaca atcttttttt cgaataaact tattttttga agtcggccat
     1141 ttatcgtaat ttctttgcat aatacgaaca aattttcggt tattgatgaa aaaaaacatt
     1201 gaaaatggta tgataaaaga aaaaaaggag gaaattttta atggtgaaat ttatccatgc
     1261 ggcagattta catatggatc ggtcatttga aggactgaca actcttgata aaactgtaca
     1321 ggaaaaacta ttgaagacga atctgaccgt tttatcaaat atcattgaac aggcaataat
     1381 aaataacgtt gattttgttt tacttgcagg agataatttt catcaaaatc gtccttcatt
     1441 aaaaatacag aaacattttt cagaacagat gaaacggtta gaaaagaacc atatacctgt
     1501 atttataata ttcggaaatc atgatttcta tcaaaaagag cggtattggt tctcctttcc
     1561 taaaaatgtc catttattta catccgaaac ggtagagaca aaaaaaataa cgataaaaag
     1621 tggagaaact gtctctctca gtggatttag ttatcgtcaa ccgtggattc aaaaagacaa
     1681 agtgatggaa tttccttcta gagaactcac agattaccat attgggttgt accacgggga
     1741 acctggcgtc tctcaaaaag gaaactatgc tcctttccaa ccctcaaaga tgcaagaaaa
     1801 gggatacgat tattgggctt tagggcatat ccatgtgcca acggtcttaa atgaaaagca
     1861 aacaattgtt tatccaggtg cgcctcaagg tcatactaaa aaggaacaag cttctacatc
     1921 tattctacta gttgaattaa gtaaaggaag ttgccaaata catccgatta aagtagcaga
     1981 ggtctattgg aagacaaagg aaatatcatt gaggaaagca agaacgacaa aagaggtcat
     2041 tgttcacatt cgtcagcagc tttcaaaagt agaggatgga tttagtttaa ttgagataaa
     2101 attaaaagat tatgaccatc tcaatacaga tgttttggaa cggatacaat caggagagct
     2161 attagactat ttgttagagg aattctcaga taggataaat gatttcttta tttggcgtat
     2221 ttctctcata gagaatactt tttatacgaa gaccccactt gctgcttctg ctaaattgat
     2281 ggaacagtta tttcaatact atcagacacc tcaagatttc caacaaattt taaatgaagt
     2341 atatagtcat caagaagcgg ctagaatatt gagcgaactg ccggaatatc aagaagaaac
     2401 acttgaaaaa gccaagcagc tgcttaatca agatttttta tttgaggagg atcaggaatg
     2461 aagattttaa ggatcgagat tgctgctttt ggaaaatggc gtcaaaaaag ttttgacttt
     2521 tattcaggca atcagctgat ctatggggga aacgaagcag gaaaatcaac gatttatcaa
     2581 tttattcaag caatattatt tggtttccct tcaaaaggcc gtaaaaaaaa ggattataca
     2641 ccaaaagacg gctcagccta tggcggcaaa atatggctta aacatcctgt atatggtgaa
     2701 tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat cgaaggtgtg gctgggggat
     2761 caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac ctttgacgaa agagttattc
     2821 cagcaggtat ttactttcca gcaagagcag ttgatggaac tagatcattt gagagaaaaa
     2881 gagctacatg atgcgctgat ttctttaggc atcacgggca gttctcaagt attccaaaag
     2941 cggcaagaat attatcagaa agcacaacaa ttattcaaga aaaaaggcca aaaattacta
     3001 atcaatcaaa aattaaatga atggaaagag ctgcaagaaa aaattcatca aaaacaagaa
     3061 caggaagctt attttggcaa gttgatccaa caagaacagg aatacgagtc gcaacagcag
     3121 cagttattgg cacaattaaa agaggcgaac aaacaatttt cattagccag acagcaagaa
     3181 cttaattttt ctttatatga agaatggcaa tctctcaaaa aaaacagcac tacaggtctt
     3241 cctgatgagc aacttctttt agatctggag tctttctcca aacggtatca gcaaacaaat
     3301 gagcgtttgg aagaattaga agcaacactg gaaaagcata gcgggatgga tcagcagtca
     3361 gccagatatt atttttattt agagcaggag caggaactaa aagatctcca gcaacttcaa
     3421 tttacagcgg aacaattaaa cgaagaatgg caaagattac aaattgagac tagagaaaca
     3481 aattcagaat tgaagatttt ggaacaaagg tggcattggc agaaaaatcc gttaccggaa
     3541 gaatttttat atgaagaaga atggaagcat cttctggctg aagaaagaag gattgctgat
     3601 ctagaaagcc agatttctgt gcgtctccaa atagctaaag aacagcaaga gatgattgaa
     3661 gcagaagtga atgacttgga agagacctat ccagcattat tacaaaatca gtcagagata
     3721 gcaccagtct cttcatctaa accgtatatg ctgatggctg gcggagcaat aatcattgca
     3781 ggtatatttg taccaatgcc cttgaaagtg atccttttac taattggtgc aggcatgatt
     3841 ggctatttct tctacaaaag gcaaccaaaa attaaaacta aaaatgattt ctctgagata
     3901 aaaggaatgt ggcaagaaaa actaggacaa cttgatctag ccttagctca attagctgaa
     3961 gcagaagaaa aacaaaagag aactggagaa gaaaaacaga ttcttcagca agaagtatat
     4021 cgttttgcgg atcatcggca cttaggaaag atggatacgc tcaaactcat gaaagaatat
     4081 ggacaggaag tcatagatta tcagaaatta tatggcgtat ttgagcggaa acataaaaga
     4141 gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa aatttgagtt tgttaaagaa
     4201 tggttaccga tacaagataa gacaatttca gaaaaaatgg tattgctttc ccgttttata
     4261 caagagatgg aacagatgaa atttgccaga agctatcagc aaaatacttt attgaagcaa
     4321 gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac aagaatacag cgaacaattg
     4381 actcaagcag gcttatctta tccttcggaa gtacctgtct ttttacaaaa agccaaacaa
     4441 gccgaacagc agatgaaaag atacaatgaa ttatcggaaa tgcttactcc actatttcct
     4501 gaagagctga caaaagagaa attatctgta agactgcgcc aactgaaaga aaagcaatcc
     4561 caactccagc aagaagaaaa agaagcaagt gaaaatcggc aacgactcca acttcaagta
     4621 gaagagttgc aaagagacgg cacattggac gaactttatc aacaagctag ccggcaaaaa
     4681 gcaaaattac aggaattact ggaggaatgg ggagcacttg aaatcgccgg gacattctta
     4741 ggtgatcttt caacagagat gagtgaaaga caattacccc agttgttaag agcagcaggt
     4801 gattatttag gtattttaac aaaaggaaat tatcagaaga tattactgaa aaatgataca
     4861 ctgactttaa cagacggata tacagaattt cccatctatg agttatctac aggtacgaaa
     4921 gatcagctga ttatggcgat gagatttgct tacctctcat tagaagctaa ccgttcgata
     4981 tgcccgatca tcattgatga tggctggctt cattatgatc atcagcgaaa gtatcaatta
     5041 gctgaacttt tagctaaatt tggagaaaag tatcaaataa tttgtttttc ttctgataaa
     5101 gaaatggtaa gctattacca tgagatgaac caaatcgtaa aacaattaga aggagcaaat
     5161 aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt gaacaatttg tcctgatcaa
     5221 aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt attgctttta ctttccaaga
     5281 tacttcaggt actatcgatg gtaaatactg ggatgcttcg gaggaggaaa tcaataaatt
     5341 tacagccggc acagtcgttt ttctaaacgg taaaagagag gtttatcaag gaaaccctca
     5401 agtaaagatt cttcatatgc gcttaacaag ggtagatgag ccaggagagc ctagtttata
     5461 catggaaaga gcccctttga agaaagaaga aatggaagaa gaaatcaatc aattgttgtt
     5521 tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat ctacttaata aatatcaaaa
     5581 acaatttttt gagtttccgg cagcaaaaag aaaccatcat gcatttgcca gtggattagc
     5641 ttatcatact ttgacaatgc ttcacttagg gaaatcaatc gccgatgaat atcctgagct
     5701 gaatcgagca ttgttgtatg ctggtatcat tttgcatgat ttaggaaagg tgattgaact
     5761 ttccggtcct atgtcgacag aatatacgct tgcaggaaat ttgatcggtc atctggtatt
     5821 gattgacgaa gaaataacta aagcctgtct tgcgttgaaa atcagcgaag ctgacgaaga
     5881 tgttctcgta ttacgtcata tggttttagc acaccatggc ttattagaat atggctcacc
     5941 tgtacgtcca agaataatgg aagcagaaat attacatcaa atcgacaata tcgatgcttc
     6001 gatgcagatg atgttgactt cgattcgtca aacagaacca ggaaaataca ccgatcggat
     6061 tttcggtatg gacaaccgaa gtttctatgt accaaaagat ttatagcaaa aatttatttt
     6121 tttcattcaa aacaaaaaaa gcgagacata agtcttcgct ttttttgttt tcttttattt
     6181 tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg attttgtgtc
     6241 agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg aagttgttgt
     6301 gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg catcttttaa
     6361 ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt cagtagcgat
     6421 atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt ttttcaccat
     6481 tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtaa taacatcgga
     6541 gatctcaccg tctttcaatt tgaaagctgc ttcttttact tctgcaggaa tagttgttgt
     6601 cgttgagtcg aatttaactt taccgccatc ttcttttgtc tctgtatcag ttgatttgtc
     6661 tttggctaat ttagagaaat catctccatc atccgctgat tttttcacat cttttgcttc
     6721 atcttcactt gataatttga tgatttgtgc ttctacttct ggatggaaag atttccaagc
     6781 agttttcaaa tcgtcatctg taatatctac gtgagctttc aagccagctt caaaagctaa
     6841 attgttacga atatattctt tataagtatc tttcgtatat ccagcagctt ctaattgtga
     6901 ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat tctgcatcca cttgcttatc
     6961 ggttacttta tcgccatatt tgttattgaa tactttataa atgatcatcc gttgtactaa
     7021 ggattggttc gatgattcaa gttttgcttc atcatagaaa tcagatacag tgatcgttcc
     7081 gcctttcatc gttgcgatat ctttattagt atcgcctgaa caagctgcta gagttaaaac
     7141 agccaacgca ctagtagctg ccaaaataat tgattttttt ttcattaaaa tttgcactcc
     7201 tgtaattagt tattattttt atatattaac tataccatat cccatttcct ttaaaaacgt
     7261 tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca ttcctaggaa atacgcaaac
     7321 ttttctcact tatatagtta aatctgttga gaaaataatc attattatat ttgttcagga
     7381 tactctggca gatcagcttt tagtttttct acttgttcat tgatttgtgt gatacgtggt
     7441 tcggcttgga atttaaaatc ttcgatatct tttttgattg cttcttggaa agcaggaatc
     7501 acagattcag ctgtttcttt tgtttcgatc aatgcgcttt taaaatgatt caggctgtta
     7561 tttaaatcat ttgtcagagt tgttgcttct tccaattctt cgatcagttt ctttcttgtt
     7621 tcattcccgc tcctgggcgc caataacaga cctccgacag tgccagcagc agctccaaat
     7681 aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc tgctttacga ttgtttcttg
     7741 aatagctttt agttcttccg atccataact atcttgatga ttgacaaagt gaatagaaaa
     7801 atcatcctct ttgctgtagc gagggactaa atggatatgt gaatgaaaaa cagattgata
     7861 ggctagttca cggttgttat tcaagatatt aagacctttc atttctggaa aagctttttc
     7921 caatgcttga gcaatcttag gcacacgcga aaaaacctct cctgcaagtt cgggatcata
     7981 ttcaaaaata tctgctacat gacgtttagg cacaactaat gtgtgtcctt tcgttgcttg
     8041 agagatgtct aaaaaagcat agaccttgtc gtcttcatat attttatagc ttgggatttc
     8101 ttgattgatg attttgcaaa aaatacagtt ttccatataa aacatccttt ctataaagaa
     8161 taaatacttc agcttaactt taccataagt taaagctatt gtaaggaaaa aaacaaaata
     8221 ccgtcatttt cttcgtggta taataaataa aaataaatgg cggaggaaaa agaatgagtt
     8281 taattatcga tcatgtcaca ggggggtacg gacatctccc tgtgttaaaa gatatcagtt
     8341 tcgatgtaca gcctggagag atagtagggt tgattggact gaacggagca gggaaaagta
     8401 cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa aggaacgata gaaatcaatc
     8461 atctatcctt aaagaaaaat acggaagaat atcgcaaaca aattgggtat attccagaga
     8521 ctccttcttt atatgaagaa ttgacattga gagaacatat cgaagtcact gcgatggcct
     8581 acgatatacc tatggatata gcaatgaaaa gagcagaaaa gttgttggag acttttcggt
     8641 tatctaatcg tcttgactgg ttcccagcta atttttcaaa gggaatgaaa cagaaagtga
     8701 tgatcttatg cgcctttttg atcgagcctt ctttatatat catcgacgaa ccatttttag
     8761 gcttggatcc actagctatc aatgcattgc ttgaattgat ggtagaaatg aaaaaagaag
     8821 gagccggtat tttgatgtct acgcatattt tagctactgc agaaaaatat tgtgatcgtt
     8881 ttgtggtctt acataacgga gagatacgag cagacggttc tcttgagatg ttgagaaaag
     8941 aatttcagct gccggattct tctttggacg aaatctatat tgctttaaca aaggagcagg
     9001 cacatgagta gtttttttca aaaacgttta gcaagacatc aaaagaaaat gatgaaatat
     9061 atgcgttatg tattgaacga tcattttgtt ttagtttgtc ttttcttgat tggcggtgtg
     9121 ggattatatt attctaattg gttgaaaacc ctgcagccac cgtttgcaat tggagggctc
     9181 ataatctctg tattttggat gctctgttta ttcgtgggta agatggctac ttttgcagag
     9241 gcagcagata ttgttttttt acttcctaaa gaaaaagaaa tgcaaaccta tttggaacga
     9301 gcttttcgct attcttgcat ttttccgttg atctttttga tattggcatg cggattggcc
     9361 atgccattag tggtggttag tacggagcaa tccttttcga tgtttttcgt ctatctgatc
     9421 atattatgga gtttgaaatt cagtcatctg caaatcaaga gagcagaatt gtttcgacta
     9481 gatcaacaag ttattcgtta ttggaaatta ggctggtttt tttcttctct gatctttttg
     9541 tttctatcat tttattggct aatatgggca ggaatagcag gtgcattagt acaggcaagt
     9601 ctatattact ggtttttgtg gaagcaacaa gaaaaaggat tggattggga gaaaatggtg
     9661 catgtagaag aacataggtt gcatcagatc tatcaattta tcaatttatt cacagatgtt
     9721 cctgaaatta cggcaaagat caaaagaaga cgctatttgg atcctatatt gactcatatt
     9781 aaaagagaat caaaaaacac ttatctttac ttgtttgcaa gaagattggc aagaggttct
     9841 gattttagtg gtctttattt ccgattagta attataggtg ggttattgat tgccggagta
     9901 gtcgactttt attttagtct gattatcggt ttcttatttt tatatttgat cggtttccaa
     9961 ctgttacctt tagcgaacca gttccgctat atggttctcg ttcagcttta tccaatccat
    10021 aagaatcaga agaagcaggc gattcagtat ctactcacat gggtattaat tatcgcgtcg
    10081 gttatttttg gtttgattgc tgcagtcgta ttagatgggc agcagcgatg ggttccgcta
    10141 gcggcttatt tgattgttac tatgctcttt gttaaactat atgtaccaac tcgtttgaag
    10201 aagatgtcag actgaaaaca aagctgtgta gggtaaaaat gagaatgaaa aatagagcgt
    10261 tgacagtaag aatgctgttc gttctatttt ttttgagaaa agctggtttt ttgaatggca
    10321 attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg ttcaggcgac tagaataata
    10381 agtactgcaa aacaaagagt aagaggacgg gagaatgcat gatggagttt cagttagacc
    10441 aagagtggcg gttacgtccc atcaaaggcg acacaggaaa aacatacata ggcatgcgtg
    10501 atgaggacaa agtctttatt aaacgaaaca cgactccgat gttggctgct ctttcaaggg
    10561 aaggaattac accaaaactt gtttggacaa aacgaacggg gaatggcgat actttgacag
    10621 cacaagagtg gctggaaggt cgcttgctga ctcctaatga gattggtcaa agaaacgacg
    10681 tgattgatgt tttgtaccat ttacatcatt caaattctct taaaaccatg ctgacccgta
    10741 tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa atatgaaaaa gaattaccgg
    10801 aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg ttttcttcgt gagcagatcc
    10861 cgtcaataga taaggaaaac ttatcagtag tacacggaga tgtaaaccat cgtaattggt
    10921 tagtatgtaa aaattattta tatctagtcg attgggattc aatcatgttt gcagaccctg
    10981 cggtggatat tgcaacgatt ttaggaaatt atgtgccact gtctagctgg aatcagtggc
    11041 tgatcagtta tggcatacgc ccgactgacg aaatgcttga aaaatttcat tggtatgcgg
    11101 ccatgaattt gctccaagaa atcaccagat attgcaaacg tgaagatcat cgacggatga
    11161 acgaagaaat tttacaatta aaacgaattt tcagtggctg aggtttggcc caaactctgc
    11221 ggagatttgg ccagaccttt ttaattagag gaaaggaatg gagataaata atgcgtgtcc
    11281 gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc gaattttgta attagtgatc
    11341 caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa cgatcatcca atccacattg
    11401 agattggtat ggggaaaggc cagttcatca ctggaatggc aaaagcccat cctgaaatca
    11461 attatattgg agtagaaatg caggtcagtg ttgtttcgat tgcactagac aagttgatcg
    11521 aacaaccgct tcctaacctg aaacttctac atgtggatgg atcagcgttg acagagtatt
    11581 tcgctgacag cgaagtagat caaatctatc tgaatttttc cgatccatgg ccaaaaaaac
    11641 gtcatgaaaa acggcgattg acatataaga cttttcttgc tgtagatgaa cagattcttc
    11701 gtccaaatgg agaaatccat ttcaaaacag ataatcaagg attgtttgag tattctctgg
    11761 caagtttttc tcaatatggc atgatcctca aacaggtatg gttagattta catcaaagtc
    11821 aatttgaagg caatattatg acggagtatg aagaaaaatt ttcttcaaaa ggtcaacgta
    11881 tttatcgagt agaagctcaa tttcaagata agaccaaaaa gaaaagtctg tgaaactaac
    11941 tttctgcttt cataaaaaca ttaatatcca taaataaatc ccagaaaatg aacattacag
    12001 atgtaatgta ttttctggga tttatttgat ttaaaatgta tttatttcat cggtttttcc
    12061 acaaggtgga atcaattgga agcgagaaaa tgaaaataat cctttgtcgg tgatcttcaa
    12121 ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa aaaggatcaa aggttttagc
    12181 tgctcctagt tgatgtgctt tagttattaa ttcattcaac tgttcataga cgatcgaaaa
    12241 aggttcctgt gtcatgattc cgcctatagg aagagaaaca caagctattt cctgctgttt
    12301 gttgacaacg gtcatcccgc cacctttttg aatcaaaaga ttagcagcat acagcatatc
    12361 ctcgtcgttt gtcccaacga caactagatt atgcgagtca tgggcaatcg ttgtcgctat
    12421 cgctccttgt ttcagctgaa atcctttcac tatgcctaat ccaatgtttc ccgtttgatg
    12481 gtgacgctcg atcaccgcaa tctttaattg gtctttttcg attgaaggaa tgaatcgttc
    12541 gccttgtcgg tccacttttt ctaccaaatc tttagtcact agactattcg ggataatttc
    12601 gatcacatgg gcttgtgaag aagaaagaag cagctctaac gaatgtggat ttaatggttg
    12661 agcgtttagt tttggcagac aacacgtata tgaatttgaa tgatcagcaa ataaatggtc
    12721 ttggagttgc ccagattgaa caactaatgt tcccttatag aaaacttcat gaattggcag
    12781 cttggtaaga tcgtctgtta agaagaaatc agcttgatac ccaggggcaa tcgcacctag
    12841 tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc atctgaatgg cttgtaatgg
    12901 atcgattcca agtgcaatag cttgacggac gatatgatcg attgatcctt cttttactaa
    12961 atcattgatc agtttatcat cggtgacaaa gaagcatctt cttgaatttt caggtgtgat
    13021 cgccggtagt agcgatgcca gatcttttgc aacggtccct tctcgaacca ttaaatacat
    13081 accaagatcc aaacgttctt tagcttcttt tgcagttgta gcttcatgat ccgtccgaat
    13141 acctgctgca ggataaacat ttaactcttc catgccgata ccagctgcat gcccgtcaat
    13201 ttttttctta tgctggtgca tcaattggat cttttctagg atgtctgttt cattatttgc
    13261 tactgcttga tagttcatga cttcagctaa gcctaaaact tcgggttttt gtaaaaaagg
    13321 ggctaatttt tttgcattta aatttgcacc attggtctca aaaggggtta caggtacaca
    13381 agaaggtagc ataaaaaaga tattcatgag tgtttgtctt gcatcttcta tcataaactc
    13441 aagacctgta ctcccagcaa tgtttcctat ttcatgggga tctgtcacta cagtagtaac
    13501 tccatgaagc aaggatactt ttgaaaattc gcttggtgat aaaagtgagc tttcgatatg
    13561 cacatggcca tcgatgaatc ctggcactag atattgccca ttagcattat gtacgctttt
    13621 tccttcgtat tcaccgattc ctacgatcat tccttcgcag attgctacat ctttttccat
    13681 gatttctttc gtaaagacgt tgacgatctt agcatgcttg atcactaggt cagcaggctt
    13741 ttttttactt gctacttcga tatatttcct taatgttgtt tccaatggag tgcctcccaa
    13801 gataaatttt tataagtata acataaattc ggtttttgcc aatatttttt tgtaaattca
    13861 ttttatttga aacaaaaaga tcaaaaagac gtatgttttt tgtggagggg agaataatgg
    13921 aaataaaacg attatcggat caaatgataa gtgaaagttt tgccaaacat gcccaagacg
    13981 tatatatatt gccagcttca gaagtatttt acatctattt tcgctatgga aaaaaacgaa
    14041 cattctggaa gaaagtctct tcggcagatg gacagcaact gatctcaaga tttaagtatt
    14101 tgggacagat ggatatcgga gaaaaaagaa aagctcagtt aggtgcattt acttattctt
    14161 tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg agattacctt caacgagaga
    14221 gtttagtgat tcggctatta tacggtactt ttgaaacacc ccatcgttgc tttagttctc
    14281 gagattttgc aactatttta cagaaaaccg tcagacgtgg attatacctt ttcagcggac
    14341 cagtcggatc aggcaagact tctctaatgt atcggttggc tttggaagaa aaattgcaag
    14401 tgattactat cgaagatcct gtagagatag aagaaccgtt ttttctgcag ctacagatca
    14461 atccgaaaat agcacagact tatgatacac ttttgagact cgcattacgg catcgtccag
    14521 acttgctaat cattggcgag ataagagatg aatcaactgc acatgctgcg gtaagagcag
    14581 cactaacagg gcaccgggtt tttgcaaccg tacatgcacg caatcttgaa ggtacattgg
    14641 tcagaatcca tgaactgaca aagaaaaaag aagagctatc tgaatgttta gctggtgtca
    14701 tttatcaaga acttttggaa gatgccaatc accatccttc tgttttatgg agttatcaat
    14761 ttatggagga agaatcagta aaaaaatcat gggaggaaag ttatgaagaa gctcaggaaa
    14821 atatttggca gtcagaacta ttctaaagga ttaacaagaa gccagcaaaa ccagtttgca
    14881 caaaccatgg gagaactgct tttatctggt tttagtctgc aagaatccct ttatttatta
    14941 ctcacgatcc gttcgtttcc acctgttatt ttggcaaata tccaacatca gctaaaggaa
    15001 gggcaagcgt ttagtgaggt actttcgcaa ataggatatg gagcagagca actggtacag
    15061 atcgagttag ctgaacgtca tgggaatctg ccgacaactt tgttggaaat tgccaaacag
    15121 tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc tgcttgcata tccgatgcta
    15181 ttgctcacct ttttgctagg tatcttgttt agtatgcggt tgttcgtttt gcctcagctt
    15241 attggttcag gaatggtcat gcaagagcat tggggtattc agctgataca agtctttcat
    15301 tggtatgttc tactattcat cctagcggtt actctattgg ctttactagt aaggtatcgt
    15361 ctgatgaagt tgtctcctgt tgatcagagt gaatggataa gcagacaatt attgattgga
    15421 ccgatatatt ccttgtatca atcggcttat ttgtcactag agattggaaa gctttttcat
    15481 gaaggtttcg aattaaggca aatcatagtc tgcttaaagg aaacgaaaca agaaagtctg
    15541 attcagcatc ttgcctctaa gttgattcaa ggattggagg caggtatgtc attggctgag
    15601 cagttccaga attatcgttt tctatcagaa gaattcagta aaattgtttt acagggagag
    15661 gcaaagggaa gtttaggtaa agaactgatt ttttacagcg aatggacaag aaaacagctt
    15721 ttcaaacgtc ttcatcactg gatgcatttt attcagccaa tcattttttt gggcgtagcg
    15781 gctttgattt tattagttta tgccgctgtt ttattacctg tttacggaag catagaggag
    15841 gttttgccat gaaaaagaga aaaatatctt attatagcgg ttttacattg atcgaaatgc
    15901 taatcgtctt actgatcatc agtgtattag tgttgttgtt cgtccctaat ttgtcgcgtt
    15961 ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat ccagttggta gacacacaaa
    16021 aagagctcta tgcgcttcaa aataatggaa gagttcctac tgtggaggaa ttgttgaatg
    16081 aaggctatat caaacgagaa catgcagaaa tctatcagcg gccttgaagg taaggtttta
    16141 gcatcaggat atacgttgtt tgaatcactc ttactgattt tattacttac aacgatcctt
    16201 tcttttccag ttctcgcatt ttctgcatgg aagaatgaat tggcagtcta tcaatttttc
    16261 ggccaatttg aaaaacgaat ctattctacg caaaaaatcg ctattgtgaa tcagatccaa
    16321 acaggctttt attggaatag tgaagagaat caaatcattt ttcatgttcc gaatccagat
    16381 aaaataaatt gggaagtatt agatattcct gatgagatta ccttgaaacg ccatgcttct
    16441 attacttttg ctgctagaac cgggaatgaa agttcactaa aagcttatca attttattgg
    16501 gaagaaaaag agcggacgat tacgtatcaa ttccagatag ggagtggtcg ctatacaaaa
    16561 agaattgaat aaaacaggat ttgttttgtt ggagtccctt acagcgctta tgatcgcttc
    16621 tgtctgtgtc tacttagtga gctttggcca gctgtttttg cttcaggcag aaaagaaaag
    16681 tggggaggaa ctgctattta ttcgaatgat ttatgaagat atcaagacgc agcggatgta
    16741 tcacaaaaga ccagagcgaa gggaagaaat gatcccatac gatatccaaa ttgtcgataa
    16801 taatggagta atcgaaaaag tgtttgtcaa tatggagaaa gaagcgatca gtattgaaaa
    16861 aacaagtaat tccagcattt acgctgattg aatgtctaat tggactgatg atattgagca
    16921 gtctattgct cacattcaat cttttgattc agcaaaccat ccaagtaagt gaatttttgc
    16981 agcgtaaaga tcaaaaagag tggctaatat ttttagcaca attagaagaa gaactcaaga
    17041 gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta cacgatcgag gataaacagt
    17101 atatcatcga aagatatcag gcaatgatac gtaaaagaag aacttccggc ggacatcaac
    17161 ctatgttaac aagtatcact gagcttcaat tattggaaaa agaacacaca atttcttttt
    17221 atgtacactt tgaaaatgga gaagatggat atgccatatg gacaaaaaac gatcagtaga
    17281 aaatgttata agggctctat tttattttct gtcttgcttt tatttctttt atgtacgttt
    17341 ttattgatgg cagcattaga aagctaccgt ttgtcaatgg atcttaatat acgaatcaaa
    17401 aattattata tagcaaaaat aatgaagcag atggctttat cggaacagtc tacgcttgcc
    17461 gaaagttcgg aaggagtttt ttactacaca actggatctg taacatatca gtgggtacaa
    17521 cagtccctat tcttagaggt ggaagtctca ccgtttatct ttcggttcac agaagaagta
    17581 aaaaatgata cagatactag tacagaatga tggaatgttc gtgaaaacgc cctcttttct
    17641 gttataacac tgtttttaat aaaaaaatgt ttgccgtttc acaaataaaa aacagtgaaa
    17701 acgattgaaa ttttgtcaat tgttcgatag aattaaacag gtgaaaaaat tgttataata
    17761 tatctagaaa tgaggtgcac catgttacca gaaaaaatgg aacaagggtt taatcaaaac
    17821 ctggaagcta ttcagctttt acagaatgca ttgggaactt cctttttaga agcctatgtc
    17881 gaaaatgcag aaaatttaat agatgattat caagtacgtg tggtggatgg agtgccgaca
    17941 aaagagacaa cgcaacggat cacagctctt tatgaagagt tgaagaaact ttctttcgag
    18001 ccagaagaat ggcgcagact gtctcaatta cttttattga agggcagcca gacagaacat
    18061 ttgcaggcga accatcaact cactcctgat agtatcggtt ttttatttgt ttttcttatt
    18121 gaacaactat acacaaataa aaaagcaccg gttaagattc tggatattgc ggcaggaatg
    18181 ggcaacttgt tattaacagt tcttttaaac ctatcgaatg cggggtacca aacagaagga
    18241 attggtgtgg atattgatga tacattgttg gctgtcgcag cttctacaag tgatttgaca
    18301 caagcaaatg ttcaatactt tcatcaagac gggttgcagg aattattaat agatcctgtc
    18361 gattttgcaa tcagcgattt gccaatcggt tattatccga atgacgaaaa agcaaaagag
    18421 tttttaacaa gtaccgaaga aggccatagt tatgcacatc atttattgct agagcaatca
    18481 atgaaatatg tgaagccgga tggttttggt ttgtttttga tgccgagtgg atttttagaa
    18541 acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag aaggttatct tcaaggaatg
    18601 attcaattgc cagatgaatt gttccgtaat aaacaatcgc aaaaaagtat tttgatctta
    18661 cagaaaaaag gaccacaagc acaacaagta aaagaagtct tgcttgtgaa acttgcttca
    18721 ttaaaagaac cagagaaggt gacggaattt ttcaacgagt ttaaaaactg gaaatcatct
    18781 gctctgtaat cattcaacga gaaggagagt catatgtcta aaacaattgc aatcaatgct
    18841 gggagttcaa gtttgaaatg gcaattatat caaatgccag aagaaaccgt agttgcgaaa
    18901 gggatcgtcg aacgtatcgg tttacaagat tctattttta ccatcaagta cggcgataat
    18961 caaaaattcg aacaaatcat ggatatcgaa aaccacgaag tagcagtaaa aatgctgtta
    19021 gatcagttga ttgaactgca aattttaagc tcttatgatg aaatcaccgg agtagggcac
    19081 cgtgttgttc acggaggaga aacttatggg gattcagtag tgatcgatga gaaagtaatg
    19141 gaccgcattg ctgaattagc agaatttgct cctttgcata atcctgcaaa cttaatgggg
    19201 atcaaagcgt ttaaaaagat tttacctgac attttgagtg tggctgtatt cgatacatct
    19261 ttccactcaa caatgccaaa gcaaaattat ttatatagct taccggtgtc ttattatgaa
    19321 gatttcaaag taagaaaata tggcttccat ggcacaagtc atcgttatgt gtcggaacgt
    19381 gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa tcatcacttg ccacttagga
    19441 aatggtgtat cgatcacagc agtcgatgga ggtaaatcca tggatacttc tatgggattc
    19501 acaccattag ctggggtgac aatgggtaca cgttcaggtg atatcgatcc agctgttctc
    19561 ccatatttaa tggaaaaatt agagattgat attgatgaaa tgatcaatgt tttgaacaag
    19621 aaatctggtc tgctaggcct aacaggattg tctagtgata tgcgtgatct tgaaaagaac
    19681 tatgaaaaag aacatattcg tttagcttat gacattttcg tggaccgcat ccgaaaatac
    19741 atcggcggtt atgtgacaac gatgaatggt gttgatgcaa ttgtctttac tgctggtatc
    19801 ggcgagaatg acggacatgt acgtagtgaa atcatcaaag gtatgtcttg gtttggttgt
    19861 gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag tagatatctc tacaaaagaa
    19921 tcaaaagtaa gagtattagt gatcccaaca gatgaggaac taatgattgc ccgtgatgtg
    19981 gagcgtctgc gcggctaagc caaagcattc gttaggataa aagatgaaac gcatgcaaga
    20041 gggaattctt gcatgtgttt tttttgcgga gaagaccgca aaatggtaat ttaacttcaa
    20101 tttcccttct tttttacagg atcttcttag ggaattttgc ttggaaaagt ggtcaaaagg
    20161 gaaaagactg gcattttatt tattgatttt caggaaaatc ccaatatttt attttcattc
    20221 atctttataa gggacattac atatgtaaaa agaaaaaaca tttttagatc agcctcgtgg
    20281 gctgtcgact ttattcttcg ctttatgttt agctagaaaa ccgatccgaa acattcattg
    20341 atcgaataat aaaaacttga ttttccacct gtactttgcc tgaatcgtga aagcacaggt
    20401 atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc aatcagttgg ccgagcggct
    20461 ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc tgcaaaaagt
    20521 agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt tcttcaattt
    20581 cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg acgttgattt
    20641 tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat aaatctttga
    20701 tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt cgagcgactt
    20761 cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga tcggcaaact
    20821 gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg gtgccaccgt
    20881 aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca ccattgtcgc
    20941 cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg aatagtactt
    21001 gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca gattttccac
    21061 cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg agtgtcagtt
    21121 tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg tctccttcat
    21181 gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct ttttgctgca
    21241 gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga aaacggctgt
    21301 agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt gtggtgactt
    21361 gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta ttctgtaaat
    21421 agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca tcgagtaaaa
    21481 gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt tcgcctccgc
    21541 taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc aagatcgctg
    21601 caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc agctcaccgt
    21661 aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct tcgtagcgac
    21721 tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag tggttgacgg
    21781 tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct tttttgcgac
    21841 tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag gttgatttgc
    21901 cagttccgtt gataccgact aaggcaattt tttcaccagc ttcaatttga caggataaat
    21961 cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt atgatcatga
    22021 tctctctcct tgagaccact caaaaaggcc atggttatcc atggcctgtg ttatttaaac
    22081 tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga attcaactgt
    22141 tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc taaatgcgat
    22201 atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca tagtattttc
    22261 cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt tcaactagcg
    22321 ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt tttatctgac
    22381 tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc gttcaggcga
    22441 aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc atttgcttaa
    22501 taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg ttcaggagta
    22561 tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga tttaccgaat
    22621 gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt ggcgataaaa
    22681 tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga aacttccaca
    22741 ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca gggcgactaa
    22801 tcgccctggt gttcactttt ttttatttta cttgaactca ttgactgttt tccctttatt
    22861 ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt
    22921 gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt ggtgtggtct
    22981 ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca agctgcgttt
    23041 ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt caatcctgtg
    23101 gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc aataatttgt
    23161 tttggtgagc catattctac gacagaggtt acattattca agccagcttt cttcgcattg
    23221 ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc aagcattcca
    23281 tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa tagaagttct
    23341 ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc ttcatcagaa
    23401 ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca ctcctcaagt
    23461 caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat tatttactgc
    23521 tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac aataattacg
    23581 atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa aatacccatt
    23641 acgcctaaaa cagcatatgc agctccgtaa aattgtagga atccttgatt tttatcattt
    23701 ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct aacgaataat
    23761 aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc caaaataagc
    23821 aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat
    23881 ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag caggtgggtc
    23941 ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac
    24001 gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc
    24061 acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca
    24121 gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact cttttatacg
    24181 gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct gataatactg
    24241 agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat caaaatgatg
    24301 aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat ggctatctct
    24361 caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa
    24421 tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga cgtctgctag
    24481 tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt tcactgtacg
    24541 agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca tcagacgccg
    24601 gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa gtgcagcatc
    24661 cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat gagcatctcc
    24721 atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg acagtgtaag
    24781 atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca gcccattcaa
    24841 tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa
    24901 ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt cgactgccaa
    24961 ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc
    25021 tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga ttcggccact
    25081 ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa
    25141 taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta gatccttttt
    25201 tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg cgatcgcact
    25261 tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag agagcatgcg
    25321 agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct gaccaacgac
    25381 ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag gattttcctc
    25441 cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag aaagaacagc
    25501 cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga
    25561 gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg acgccagaag
    25621 tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga agattagctg
    25681 gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc catagctatg
    25741 gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt
    25801 acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg attcctcaag
    25861 gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt aaaaatcgtg
    25921 gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa gcattagcaa
    25981 ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta gtttttgaga
    26041 aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca aacaaaggtg
    26101 tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc tctttacaaa
    26161 gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta
    26221 ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt agctttagat
    26281 tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga aaataaagca
    26341 caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa
    26401 gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa ttatctagca
    26461 gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc tttagtggaa
    26521 tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt ggatgaagac
    26581 gatgacgaag ctgtattcga gcttcactgg gataatcagc aactagcaga attagtagaa
    26641 gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta aggagtgaca
    26701 gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga agcagttgct
    26761 aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt agatgttgaa
    26821 cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac atcgctgaaa
    26881 gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga aatcttgcct
    26941 tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg aaaaaataaa
    27001 gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa atactatcat
    27061 ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta ccagacagaa
    27121 gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac aggcactcat
    27181 cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg tgaaacattg
    27241 ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct tggtgcaaaa
    27301 gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga aaatatggat
    27361 atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac aggaatcgag
    27421 attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatccca
    27481 gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat cattgaagag
    27541 aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga tcaaattttc
    27601 cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcgg
    27661 tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg ggaaaactat
    27721 caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt ggcattcaat
    27781 gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt ttatctgaaa
    27841 gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc
    27901 tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt aggagcgcaa
    27961 gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg
    28021 aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc ccatcggcag
    28081 atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg gctagatgat
    28141 tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca
    28201 gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt tggaccagaa
    28261 ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat actttgtgga
    28321 ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag tgctgctagc
    28381 tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat aaaagagtat
    28441 cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat tatttataca
    28501 atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg
    28561 tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat tcccgtttta
    28621 ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa cgggacccgg
    28681 tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg ttgagaaagc
    28741 atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg aaccctacat
    28801 tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc cccatacagt
    28861 ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag aagatctgag
    28921 agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat tagggaaaat
    28981 aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc tgctagcaat
    29041 ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata acatgcgtac
    29101 gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt tagaaatcta
    29161 tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag aagatacagc
    29221 acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc agacgaagag
    29281 agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag cgacagaaga
    29341 gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa tctatcgtaa
    29401 aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta ttcgagtgat
    29461 cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa aatggaaacc
    29521 aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt accaatcttt
    29581 gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc gaacacagga
    29641 aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag aaggaaaaaa
    29701 tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg agatactcga
    29761 acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag gagatatctt
    29821 tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac caaaaggatc
    29881 cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa ccactggtgc
    29941 aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag atatcattga
    30001 gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat tagttgctac
    30061 tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt
    30121 gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta cgcctaaaga
    30181 tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc aaacagaaga
    30241 tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg ccaatcgtct
    30301 gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat
    30361 catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt
    30421 cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc cgattcctgg
    30481 tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc gtcgagattg
    30541 tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag tcgaatggga
    30601 agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg gctataatcg
    30661 ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag
    30721 cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg ttggtatcca
    30781 gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg atgtctacag
    30841 tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa cgggtaagta
    30901 aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc ctacttgttt
    30961 tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta aaaaaaattg
    31021 cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg
    31081 gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa aaaggaaatc
    31141 gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac gaatcattca
    31201 atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt
    31261 cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa aacaaataaa
    31321 gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag actgataaac
    31381 tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa
    31441 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
    31501 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
    31561 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
    31621 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
    31681 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
    31741 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
    31801 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
    31861 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
    31921 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
    31981 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
    32041 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
    32101 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
    32161 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
    32221 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
    32281 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
    32341 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
    32401 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
    32461 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
    32521 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
    32581 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
    32641 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
    32701 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
    32761 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
    32821 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
    32881 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
    32941 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
    33001 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
    33061 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
    33121 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
    33181 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
    33241 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
    33301 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
    33361 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
    33421 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
    33481 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
    33541 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
    33601 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
    33661 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
    33721 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
    33781 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
    33841 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
    33901 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
    33961 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
    34021 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
    34081 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
    34141 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
    34201 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
    34261 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
    34321 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
    34381 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
    34441 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
    34501 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
    34561 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
    34621 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
    34681 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
    34741 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
    34801 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
    34861 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
    34921 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
    34981 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
    35041 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
    35101 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
    35161 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
    35221 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
    35281 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
    35341 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
    35401 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
    35461 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
    35521 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
    35581 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
    35641 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
    35701 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
    35761 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
    35821 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
    35881 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
    35941 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
    36001 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
    36061 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
    36121 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
    36181 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
    36241 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
    36301 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
    36361 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
    36421 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
    36481 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
    36541 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
    36601 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
    36661 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
    36721 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
    36781 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
    36841 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
    36901 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
    36961 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
    37021 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
    37081 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
    37141 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
    37201 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
    37261 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
    37321 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
    37381 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
    37441 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
    37501 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
    37561 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
    37621 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
    37681 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
    37741 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
    37801 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
    37861 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
    37921 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
    37981 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
    38041 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
    38101 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
    38161 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
    38221 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
    38281 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
    38341 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
    38401 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
    38461 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
    38521 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
    38581 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
    38641 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
    38701 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
    38761 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
    38821 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
    38881 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
    38941 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
    39001 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
    39061 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
    39121 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
    39181 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
    39241 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
    39301 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
    39361 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
    39421 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
    39481 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
    39541 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
    39601 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
    39661 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
    39721 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
    39781 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
    39841 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
    39901 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
    39961 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
    40021 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
    40081 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
    40141 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
    40201 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
    40261 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
    40321 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
    40381 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
    40441 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
    40501 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
    40561 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
    40621 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
    40681 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
    40741 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
    40801 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
    40861 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
    40921 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
    40981 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
    41041 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
    41101 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
    41161 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
    41221 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
    41281 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
    41341 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
    41401 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    41461 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    41521 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    41581 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    41641 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    41701 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    41761 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    41821 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    41881 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    41941 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    42001 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    42061 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    42121 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    42181 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    42241 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    42301 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    42361 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    42421 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    42481 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    42541 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    42601 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    42661 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    42721 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    42781 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    42841 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    42901 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    42961 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    43021 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    43081 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    43141 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    43201 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    43261 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    43321 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    43381 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    43441 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    43501 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    43561 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    43621 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    43681 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    43741 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    43801 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    43861 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    43921 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    43981 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    44041 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    44101 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    44161 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    44221 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    44281 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    44341 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    44401 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    44461 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    44521 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    44581 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    44641 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    44701 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    44761 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    44821 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    44881 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    44941 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    45001 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    45061 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    45121 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
    45181 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    45241 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    45301 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    45361 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    45421 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    45481 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    45541 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    45601 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    45661 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    45721 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    45781 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    45841 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    45901 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    45961 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    46021 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    46081 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    46141 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    46201 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    46261 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    46321 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    46381 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    46441 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    46501 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    46561 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    46621 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    46681 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    46741 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    46801 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    46861 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    46921 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    46981 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    47041 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    47101 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    47161 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    47221 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    47281 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    47341 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    47401 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    47461 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    47521 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    47581 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    47641 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    47701 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    47761 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    47821 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    47881 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    47941 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    48001 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    48061 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    48121 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    48181 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    48241 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    48301 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    48361 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    48421 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    48481 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    48541 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    48601 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    48661 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    48721 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    48781 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    48841 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    48901 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    48961 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    49021 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    49081 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    49141 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    49201 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    49261 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    49321 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    49381 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    49441 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    49501 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    49561 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    49621 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    49681 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    49741 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    49801 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    49861 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    49921 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    49981 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    50041 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    50101 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    50161 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    50221 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    50281 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    50341 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    50401 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    50461 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    50521 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    50581 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    50641 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    50701 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    50761 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    50821 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    50881 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    50941 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    51001 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    51061 tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacag
//
LOCUS       DACCIQ010000011        50269 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCIQ010000011 DACCIQ010000000
VERSION     DACCIQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.011"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..620)
                     /locus_tag="IVM62_000639"
     CDS             complement(<1..620)
                     /locus_tag="IVM62_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844624.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYP"
     gene            complement(799..1518)
                     /locus_tag="IVM62_000640"
     CDS             complement(799..1518)
                     /locus_tag="IVM62_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6844625.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1649..2800)
                     /locus_tag="IVM62_000641"
     CDS             complement(1649..2800)
                     /locus_tag="IVM62_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6844626.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3017..3757)
                     /locus_tag="IVM62_000642"
     CDS             complement(3017..3757)
                     /locus_tag="IVM62_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6844627.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3761..4084)
                     /gene="rsfS"
                     /locus_tag="IVM62_000643"
     CDS             complement(3761..4084)
                     /gene="rsfS"
                     /locus_tag="IVM62_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP6844628.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4141..4734)
                     /locus_tag="IVM62_000644"
     CDS             complement(4141..4734)
                     /locus_tag="IVM62_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6844629.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4718..5365)
                     /locus_tag="IVM62_000645"
     CDS             complement(4718..5365)
                     /locus_tag="IVM62_000645"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP6844630.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5378..5689)
                     /gene="yhbY"
                     /locus_tag="IVM62_000646"
     CDS             complement(5378..5689)
                     /gene="yhbY"
                     /locus_tag="IVM62_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP6844631.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5705..6814)
                     /gene="yqeH"
                     /locus_tag="IVM62_000647"
     CDS             complement(5705..6814)
                     /gene="yqeH"
                     /locus_tag="IVM62_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP6844632.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6811..7338)
                     /locus_tag="IVM62_000648"
     CDS             complement(6811..7338)
                     /locus_tag="IVM62_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP6844633.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7518..8303)
                     /locus_tag="IVM62_000649"
     CDS             complement(7518..8303)
                     /locus_tag="IVM62_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP6844634.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8296..9165)
                     /locus_tag="IVM62_000650"
     CDS             complement(8296..9165)
                     /locus_tag="IVM62_000650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP6844635.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9166..10545)
                     /locus_tag="IVM62_000651"
     CDS             complement(9166..10545)
                     /locus_tag="IVM62_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP6844636.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10553..10972)
                     /gene="fabZ"
                     /locus_tag="IVM62_000652"
     CDS             complement(10553..10972)
                     /gene="fabZ"
                     /locus_tag="IVM62_000652"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6844637.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10997..11473)
                     /gene="accB"
                     /locus_tag="IVM62_000653"
     CDS             complement(10997..11473)
                     /gene="accB"
                     /locus_tag="IVM62_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP6844638.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11476..12711)
                     /gene="fabF"
                     /locus_tag="IVM62_000654"
     CDS             complement(11476..12711)
                     /gene="fabF"
                     /locus_tag="IVM62_000654"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP6844639.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12730..13467)
                     /gene="fabG"
                     /locus_tag="IVM62_000655"
     CDS             complement(12730..13467)
                     /gene="fabG"
                     /locus_tag="IVM62_000655"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP6844640.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13479..14396)
                     /gene="fabD"
                     /locus_tag="IVM62_000656"
     CDS             complement(13479..14396)
                     /gene="fabD"
                     /locus_tag="IVM62_000656"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6844641.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14503..14727)
                     /locus_tag="IVM62_000657"
     CDS             complement(14503..14727)
                     /locus_tag="IVM62_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6844642.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14780..15742)
                     /locus_tag="IVM62_000658"
     CDS             complement(14780..15742)
                     /locus_tag="IVM62_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP6844643.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15752..16192)
                     /locus_tag="IVM62_000659"
     CDS             complement(15752..16192)
                     /locus_tag="IVM62_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6844644.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16498..17463
                     /locus_tag="IVM62_000660"
     CDS             16498..17463
                     /locus_tag="IVM62_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6844645.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17520..17750)
                     /locus_tag="IVM62_000661"
     CDS             complement(17520..17750)
                     /locus_tag="IVM62_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP6844646.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17836..18723)
                     /locus_tag="IVM62_000662"
     CDS             complement(17836..18723)
                     /locus_tag="IVM62_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6844647.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18812..20035)
                     /locus_tag="IVM62_000663"
     CDS             complement(18812..20035)
                     /locus_tag="IVM62_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6844648.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20052..21131)
                     /locus_tag="IVM62_000664"
     CDS             complement(20052..21131)
                     /locus_tag="IVM62_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6844649.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(21262..22989)
                     /gene="ptsP"
                     /locus_tag="IVM62_000665"
     CDS             complement(21262..22989)
                     /gene="ptsP"
                     /locus_tag="IVM62_000665"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP6844650.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22989..23255)
                     /locus_tag="IVM62_000666"
     CDS             complement(22989..23255)
                     /locus_tag="IVM62_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP6844651.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23734..25968
                     /locus_tag="IVM62_000667"
     CDS             23734..25968
                     /locus_tag="IVM62_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6844652.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            26116..26436
                     /locus_tag="IVM62_000668"
     CDS             26116..26436
                     /locus_tag="IVM62_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6844653.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(26641..26864)
                     /locus_tag="IVM62_000669"
                     /pseudo
     CDS             complement(26641..26864)
                     /locus_tag="IVM62_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(27062..28642)
                     /locus_tag="IVM62_000670"
     CDS             complement(27062..28642)
                     /locus_tag="IVM62_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP6844654.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(29043..30419)
                     /locus_tag="IVM62_000671"
     CDS             complement(29043..30419)
                     /locus_tag="IVM62_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6844655.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(30547..31665)
                     /locus_tag="IVM62_000672"
     CDS             complement(30547..31665)
                     /locus_tag="IVM62_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6844656.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31807..32241
                     /locus_tag="IVM62_000673"
     CDS             31807..32241
                     /locus_tag="IVM62_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844657.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(32319..32852)
                     /locus_tag="IVM62_000674"
     CDS             complement(32319..32852)
                     /locus_tag="IVM62_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP6844658.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32946..34124)
                     /gene="mutY"
                     /locus_tag="IVM62_000675"
     CDS             complement(32946..34124)
                     /gene="mutY"
                     /locus_tag="IVM62_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP6844659.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(34117..34914)
                     /gene="recX"
                     /locus_tag="IVM62_000676"
     CDS             complement(34117..34914)
                     /gene="recX"
                     /locus_tag="IVM62_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP6844660.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            35021..36403
                     /gene="rlmD"
                     /locus_tag="IVM62_000677"
     CDS             35021..36403
                     /gene="rlmD"
                     /locus_tag="IVM62_000677"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6844661.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(36577..37155)
                     /locus_tag="IVM62_000678"
     CDS             complement(36577..37155)
                     /locus_tag="IVM62_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP6844662.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            37477..37947
                     /locus_tag="IVM62_000679"
     CDS             37477..37947
                     /locus_tag="IVM62_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP6844663.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(38055..39281)
                     /locus_tag="IVM62_000680"
     CDS             complement(38055..39281)
                     /locus_tag="IVM62_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP6844664.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(40035..40235)
                     /locus_tag="IVM62_000681"
     CDS             complement(40035..40235)
                     /locus_tag="IVM62_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6844665.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(40369..40977)
                     /locus_tag="IVM62_000682"
     CDS             complement(40369..40977)
                     /locus_tag="IVM62_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP6844666.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(41062..41532)
                     /locus_tag="IVM62_000683"
     CDS             complement(41062..41532)
                     /locus_tag="IVM62_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP6844667.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(41580..42494)
                     /locus_tag="IVM62_000684"
     CDS             complement(41580..42494)
                     /locus_tag="IVM62_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6844668.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            42640..43443
                     /locus_tag="IVM62_000685"
     CDS             42640..43443
                     /locus_tag="IVM62_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP6844669.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            43621..44568
                     /locus_tag="IVM62_000686"
     CDS             43621..44568
                     /locus_tag="IVM62_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6844670.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(44636..44929)
                     /locus_tag="IVM62_000687"
     CDS             complement(44636..44929)
                     /locus_tag="IVM62_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP6844671.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(44957..45622)
                     /locus_tag="IVM62_000688"
     CDS             complement(44957..45622)
                     /locus_tag="IVM62_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6844672.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(45762..46394)
                     /locus_tag="IVM62_000689"
     CDS             complement(45762..46394)
                     /locus_tag="IVM62_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6844673.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(46401..47468)
                     /locus_tag="IVM62_000690"
     CDS             complement(46401..47468)
                     /locus_tag="IVM62_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6844674.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(47465..48196)
                     /locus_tag="IVM62_000691"
     CDS             complement(47465..48196)
                     /locus_tag="IVM62_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6844675.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(48365..48844)
                     /gene="greA"
                     /locus_tag="IVM62_000692"
     CDS             complement(48365..48844)
                     /gene="greA"
                     /locus_tag="IVM62_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6844676.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(48980..50155)
                     /gene="mltG"
                     /locus_tag="IVM62_000693"
     CDS             complement(48980..50155)
                     /gene="mltG"
                     /locus_tag="IVM62_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6844677.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        14442 a        10599 c         8406 g        16822 t
ORIGIN      
        1 tcaggatatt ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga
       61 tattctgggt caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa
      121 attcgattcc attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca
      181 caatccaaaa tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt
      241 ttcgtgtcta tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc
      301 cactctaata gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct
      361 gaatcagaac gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt
      421 gcgcggtcca ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct
      481 aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt
      541 acgttcacag ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct
      601 aaagtgattt ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc
      661 acatgatact tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac
      721 aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt
      781 ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc
      841 ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag
      901 tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt
      961 ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg
     1021 tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat
     1081 ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac
     1141 cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc
     1201 tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa
     1261 tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc
     1321 tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag
     1381 tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc
     1441 aaattttgca taaatccggc tattattagc atctttagca gttttctttg ctacgatatt
     1501 tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa
     1561 aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc
     1621 ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct
     1681 gttccggaat attgcctccc aattgataga tttgatcact tctaactaac agttccgctt
     1741 cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga
     1801 atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag
     1861 gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc
     1921 atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag
     1981 ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca
     2041 gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc caagtctgca
     2101 gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc
     2161 cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt
     2221 tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact
     2281 tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg
     2341 taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt
     2401 gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc
     2461 cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt
     2521 ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc
     2581 attcgacggg aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat
     2641 gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa
     2701 tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt
     2761 gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt
     2821 atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa
     2881 ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc
     2941 aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact
     3001 cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg
     3061 atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca
     3121 gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa
     3181 taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt
     3241 gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat
     3301 agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc
     3361 ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat
     3421 ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg
     3481 ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct
     3541 ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt
     3601 ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt
     3661 cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca
     3721 cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa
     3781 gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg
     3841 catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt
     3901 ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg
     3961 taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca
     4021 aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt
     4081 caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc
     4141 ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc
     4201 ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc
     4261 agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata
     4321 gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac
     4381 ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc
     4441 gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc
     4501 tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa
     4561 tgcagcaata ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt
     4621 ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg
     4681 catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc
     4741 cttcattttg aatataatca attactttat ctggaacaag gtagcggata gaacagcctt
     4801 ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa
     4861 ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca
     4921 tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga
     4981 taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg
     5041 tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt
     5101 ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa
     5161 catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag
     5221 cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc
     5281 ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa
     5341 caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg
     5401 atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga
     5461 tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct
     5521 catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa
     5581 tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat
     5641 gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc
     5701 ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa
     5761 ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt
     5821 ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag
     5881 gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg
     5941 ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga
     6001 taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa
     6061 ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt
     6121 gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta
     6181 aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt
     6241 gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta
     6301 ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg
     6361 tcatctcatg agatttctta gcactagtta ataatacatc tactggacga attcctgctt
     6421 cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata
     6481 tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg
     6541 ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt
     6601 gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct
     6661 cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt
     6721 tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg
     6781 caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc
     6841 agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt
     6901 cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt
     6961 ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa
     7021 atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac
     7081 atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac
     7141 aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt
     7201 tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat
     7261 gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg
     7321 tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta
     7381 ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt
     7441 ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat
     7501 ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta
     7561 tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt
     7621 gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg
     7681 attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg
     7741 cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc
     7801 gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca
     7861 ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt
     7921 ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct
     7981 gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa
     8041 gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga
     8101 tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca
     8161 ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg
     8221 aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg
     8281 acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag
     8341 cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga
     8401 taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat
     8461 gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc
     8521 cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa
     8581 aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga
     8641 aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact
     8701 ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact
     8761 tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc
     8821 tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc
     8881 cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa
     8941 acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc
     9001 gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt
     9061 tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct
     9121 attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat
     9181 ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc
     9241 acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat
     9301 tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt
     9361 atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc
     9421 cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg
     9481 gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc
     9541 gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc
     9601 tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc
     9661 cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa
     9721 ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc
     9781 tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg
     9841 attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc
     9901 acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc
     9961 atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc
    10021 ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat
    10081 cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc
    10141 agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt
    10201 atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc
    10261 acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc
    10321 ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc
    10381 ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc
    10441 tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg
    10501 tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa
    10561 tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc
    10621 ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg
    10681 gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct
    10741 tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca
    10801 atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg
    10861 ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca
    10921 aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc
    10981 ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa
    11041 ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat
    11101 tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta
    11161 cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg
    11221 aaacaacctc gattccatca aatactgccg attctttttc tttcattatt tctggagcag
    11281 ttgttgcttg ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt
    11341 gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca aattgacctt
    11401 cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt
    11461 cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc
    11521 aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac
    11581 aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg
    11641 atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg
    11701 tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat
    11761 ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat
    11821 atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga
    11881 tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta
    11941 tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag
    12001 aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat
    12061 cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat
    12121 ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg
    12181 tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat
    12241 agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc
    12301 aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat
    12361 aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg
    12421 gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta
    12481 gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc
    12541 gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt
    12601 agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc
    12661 atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct
    12721 ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat
    12781 atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg
    12841 ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat
    12901 atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc
    12961 tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg
    13021 accaatattt ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg
    13081 cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc
    13141 cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc
    13201 ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg
    13261 tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc
    13321 ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc
    13381 tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat
    13441 aaatacattt tttcctttta catccataac gttcctcctt atagtattgc tattgtttca
    13501 tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata
    13561 aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt
    13621 aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc
    13681 gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt
    13741 tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata
    13801 gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt
    13861 aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt
    13921 gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg
    13981 atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct
    14041 tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca
    14101 ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg
    14161 attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt
    14221 gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag
    14281 ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct
    14341 tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt
    14401 ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc
    14461 ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc
    14521 tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc
    14581 aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc
    14641 ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat
    14701 gatttcttga attttttcga ataccataat aatatattcc tccgatattg tttgcttttt
    14761 atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc
    14821 catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg
    14881 cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg
    14941 tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt
    15001 ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt
    15061 ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg
    15121 aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg
    15181 agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg
    15241 cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc
    15301 aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc
    15361 ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat
    15421 caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg
    15481 tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat
    15541 ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg
    15601 ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca
    15661 tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag
    15721 taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca
    15781 agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt
    15841 tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg
    15901 ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt
    15961 tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg
    16021 ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg
    16081 tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat
    16141 cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc
    16201 gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt
    16261 caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc
    16321 atttaaaaca actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct
    16381 cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt
    16441 gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg
    16501 ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga
    16561 gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat
    16621 tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat
    16681 tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc
    16741 caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta
    16801 catattacca ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct
    16861 tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg
    16921 ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa
    16981 atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag
    17041 ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa
    17101 gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa
    17161 caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag
    17221 agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct
    17281 ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt
    17341 caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta
    17401 gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat
    17461 tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt
    17521 tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc
    17581 tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta
    17641 cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg
    17701 accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct
    17761 ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct
    17821 aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt
    17881 gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt
    17941 caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat
    18001 ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat
    18061 acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat
    18121 cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt
    18181 catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga
    18241 accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac
    18301 ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc
    18361 ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc
    18421 tgccaggctt ggtgtatttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga
    18481 aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt
    18541 aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc
    18601 tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc
    18661 ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt
    18721 cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa
    18781 caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt
    18841 tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa
    18901 ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa
    18961 gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg
    19021 ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc
    19081 ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat
    19141 aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat
    19201 aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca
    19261 gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc
    19321 tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt
    19381 tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg
    19441 actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt
    19501 cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa
    19561 taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt
    19621 attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat
    19681 acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa
    19741 gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa
    19801 ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg
    19861 gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg
    19921 taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg
    19981 tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt
    20041 tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga gactttttca
    20101 ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct
    20161 tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg
    20221 atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac
    20281 cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct
    20341 aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa
    20401 tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga
    20461 tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac
    20521 caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta
    20581 cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat
    20641 tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg
    20701 gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt
    20761 gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata
    20821 ggagaaagct ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca
    20881 tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt
    20941 ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata
    21001 ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca
    21061 agcatctttt cactttcaaa atataataat accttcacct tacgtccccc ttcacttata
    21121 tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta
    21181 ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca
    21241 caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc
    21301 catcatatcg cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa
    21361 gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag
    21421 acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc
    21481 tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga
    21541 tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt
    21601 gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc
    21661 tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac
    21721 ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg
    21781 gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc
    21841 acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat
    21901 acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag
    21961 ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc
    22021 taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat
    22081 atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac
    22141 tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac
    22201 agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc
    22261 tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc
    22321 gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat
    22381 tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc
    22441 tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc
    22501 atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa
    22561 taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata
    22621 agcattatct tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc
    22681 tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg
    22741 atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg
    22801 cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa
    22861 acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg
    22921 aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat
    22981 ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca
    23041 tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa
    23101 gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca
    23161 tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca
    23221 gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt
    23281 gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc
    23341 ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc attcaatttc
    23401 agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac
    23461 aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc
    23521 ctttgaccct ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta
    23581 taataagagt caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat
    23641 ctcttaaaaa actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat
    23701 gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat
    23761 gaggcaacga ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt
    23821 cagagctgtt atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa
    23881 aatgatccat ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg
    23941 caacagcaag gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc
    24001 aatggtggtc agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat
    24061 atcacagaac aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc
    24121 aaacgagtca ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa
    24181 ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat
    24241 gtaccgcaaa aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa
    24301 ggtactggta tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt
    24361 caagcagaaa atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct
    24421 gctggtgatg gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa
    24481 ttgcaaatgg ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct
    24541 ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt
    24601 attttgaaag gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag
    24661 gccatcgaag cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat
    24721 aaagccatcg atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt
    24781 gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct
    24841 cgagaagaag atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa
    24901 atgaaagaac gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa
    24961 aaaatcgttg aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca
    25021 caactgaaaa acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta
    25081 gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca
    25141 atcggctcct tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa
    25201 cttgcctacg aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat
    25261 atggaaaaac atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa
    25321 gaagctggtc aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat
    25381 gaagtagaaa aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt
    25441 cgattgacag atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca
    25501 agtaatgccg gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt
    25561 gttactcgtt ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt
    25621 tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca
    25681 ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct
    25741 acgaatgtca aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct
    25801 cttcgacgca cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac
    25861 ccttctatcc atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct
    25921 aaaccagaac gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat
    25981 agcgtgagac ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa
    26041 tactgttcat tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata
    26101 aaagaggtga gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta
    26161 aaccagcttg aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact
    26221 attgtctaca ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa
    26281 atccagccga aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc
    26341 gtttaccacg aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt
    26401 ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa
    26461 agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    26521 gaaagcagaa gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt
    26581 tttgtgaatt ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt
    26641 ctagcttttt gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg
    26701 cgtgtcttta tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat
    26761 ttgctcgttt ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc
    26821 tttttctgtt tttttctttc acgtctttgc cattctgttt ccatgtgtca cactctcctt
    26881 tctttaatca tatgtacgca aaataatttt tatatttttt tgattagaca tttctatgat
    26941 tttaatatag taaaaagagc cgtcacggaa aatccgtcac ggctctaaac aaaatatgag
    27001 aggctgtttt tgtttggcta ttcttattac ttgatgcagg gcagcttgtg tcaagacttc
    27061 tttacatcaa actttttaat tttacattgg gatatttatc cgcaaaccag cgttctgcaa
    27121 attggttctc gaataagaac aacggttggt cgaaacggtc acgagccaag atattgcgac
    27181 ttgaactcat cttctcatcc aaatcttctg gatcgatcca gcgagcgatt ttgtgtccca
    27241 ttggagacat gattacttcg gcgttatact catttaacat acgatattgg aatacttcga
    27301 attgtagctg accaacagct ccaatgatat attcttccgt tagatatgtt ttataaagct
    27361 gaatcgctcc ttcttgaact aattgataga ttcctttgtg gaaggatttt tgtttcatga
    27421 cattttttgc agtcactttc ataaacagct ctggagtaaa tgaagggagt tcttcataag
    27481 caacttttag ttttccttcg aacaatgtat ccccaatctg ataattgcct gtatcatata
    27541 caccgatgat atcgcctgct actgcttttt cgacgttttc tctcgaatct gccataaatt
    27601 gtgtgacatt gcttaatttc agttttttat ttgtacgttc caaccagaca tccattcctc
    27661 gttcgaatgt tccagaacag attcggacaa aagcgattcg gtcacgatgg gctggattca
    27721 tatttgcttg gatcttgaat acgaaacctg aaaattcttc ctcataagga cttaccatct
    27781 gatcatcttg tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa gtctctaaaa
    27841 aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc tgatcgccac
    27901 gagcaatttt ttcttgatta aatgcatctc cagcctcttt gattagttct acttcttcta
    27961 agacttgttc atataaggaa ttttcagtta gaggatgatt ttcgggaagc agtccatctt
    28021 catccaattc tactaatcgt tcatcctgat aattttccgg acgataaaac tctacacgat
    28081 tatggtagat atcataaaga ccttctagcc ctttacccat accgattggc caattcatcg
    28141 gataagattc gatatctaat agctcttcta attcttctaa caattccaat ggttcacgac
    28201 catcacgatc tagtttgttg ataaaagtaa atatcgggat gccacgtttt tttacaactt
    28261 ggaataattt ctttgtctgt gcttcgatcc cttttgcact atcgattacc atgaccgcac
    28321 tatccacggc catcaaagta cgataagtat cttcagaaaa atcttcatgt cctggtgtat
    28381 ccaagatatt tactcgtttc ccttgataat caaactgcat aacagaacta gtaacagaaa
    28441 tcccacgttg tttctcgatt tccatccagt ctgatttggc aaaattacct gtttttttgc
    28501 cttttacggt cccggcttga cggatagccc caccaaacaa tagtaattgt tctgtaatcg
    28561 ttgttttccc agcatccgga tgggaaatga tcgcaaaagt tctgcgattg tccacttgtt
    28621 gctttaattt tggattattc ataacctaac tccatttctt ccattcaatt atttatattt
    28681 tctctaggca cagattgtcc gatctcttaa agaaagacaa tttcatatag ggctgtgaca
    28741 aaagccgtgt agcagtctct ttttccgagt aaacggcgtt ctagagacag cctatcagca
    28801 ataagcccac ccaagccaaa aatacgaaaa gctgtttttg cttggctgct cttattactt
    28861 aatgcaggat agctttttca caacctttgt caactactac aactgaatca gtatagcatg
    28921 gattcagcga atgaaaaagc caccaaacca taagagtcaa atattttttt gtcagctgac
    28981 taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg tctttcataa ccaagtttcc
    29041 aattatcttc ttggctcgtc atcttcgaat tcttttctga agtatcttcg ttcttcttca
    29101 ggttccgctt ctgtttcttc cggatcatga aaaatgacac ggattttaag gattcgagaa
    29161 ccttccattt cttcagaaac cagcgtaata ttatcgacat caaacgataa cttttcgcct
    29221 tcatccggaa tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc tacatcactc
    29281 atatgcagat cactgtcaaa agcttcattg aactcatcaa tcagcatacg tccttggatg
    29341 atatattcgt gttcatcgat tttttcataa agattctcaa cttcatctga ttcatcatcg
    29401 atttctccga cgatttcttc taaaaggtct tctagtgttg ctaacccaac gacgccgcca
    29461 tattcatcta ataaaatagc catctgattc tgcgtctttt tcatttcata taacagatca
    29521 tcaataaaga ccgtttcagg aacgaataaa ggctcctgca tgattttctt gatatctaaa
    29581 ttatcaaagc cgaatttatg ggcagctttt agtaaattct ttgtatgaag gattccgact
    29641 actttatctt tgtcttcgtt ataaacaggg attcttgaat agttttcgga taaaacttca
    29701 ttcacatttt cttccacagt gtcgttgata tcaatcataa atgcatccgt tcgtgggacc
    29761 atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat ttctaattct
    29821 tcattattca atacgccttc gttttcaagc atataacgca tttcatctct ggtcattttt
    29881 gaatcttcat catcaaaagt catcggcgtg atacgcgcca ataaacttgt ggaagcagaa
    29941 agcagccaga cgaagggctt agctactaca ccaatcaagc gtatcagccc tgaagtgaac
    30001 tttgcgactt cttctgattt atttaaagca atacgtttag ggtacaattc tccaaaaaca
    30061 atcgatacat aggtcaaaat agccagcaca atgatatttg caatattttt tgccgctgtg
    30121 ccgccaccta ataccggagc taaccgttcc gataaagtat tagctagtga agcaccagac
    30181 aaaatgttga ccaaagtgat tcctacttgg attgtcgata ggaaattatt tggattctgc
    30241 aatattttta aaagcttttg agatttcaca tctccttcat cagctttttg ctcgattcga
    30301 ttcttattga tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt gatcaatgtc
    30361 aaaacgatca ataataaaat ctgcgctaac agcgactgac tctcagggtc agcattcata
    30421 atcgcattct ctccttataa aattaatttt tagtttacga tagaaaaaaa cgtggcacta
    30481 tagccacgtt aaaatggtat cacaaagcaa ttttctttgc aataaaaaca agtgtactaa
    30541 aactacctac gaggtcgtag caattttctt cttgttttca ttttgcttgt cttctttgac
    30601 gattttgatt atataggaaa tgatcgtctt gcagattgcg taaaaaggaa cacctaagaa
    30661 gataccaagc aatccggcaa tatttccagc tactagaaga acgataatga tcgttaatgg
    30721 atggattttc agtgatttcc caattacatt aggataaatg atattcccat ccaactgttg
    30781 gacgattagc acaaccacac aacaaagtaa tgcttttacc ggatcattga agactgttac
    30841 tagaaatgca ggtgctagtc ctagataagg tcctaaatat ggaattaggt ttgtcaatcc
    30901 cgcaatgacc ccaaataaga aagcataatc cacaccaatt accatatatc ctaaaaatgt
    30961 gaaggtacca acgaataaac attcgattgc ttgtccgcta atgtaattag ccaatgtttt
    31021 attcagttgc cccaataact ccacaatttg gtttcttctt ttttctggga aaaactgttg
    31081 gatgtttggc actagttttt ctccgtcttt caacatatag aacaagatga atggtgctgt
    31141 aatgattact acggttgccg aagcgatcgt tccaacgatc gaacctaaac tattggatac
    31201 tccactaagg aattgctgaa tgattgtccc gtaagaaatg tctaattgct caaaatattt
    31261 attgatatct acttccttga acaaaggcaa ttcggctacc tcttttaacc agctttctac
    31321 ctgtttgatg aaatcaggca tattagatgc caatgaggaa atttgctgta ctagacttgg
    31381 gatgacacta agtaaaatcc aaataattgc tgcaactaaa aggagcagca caattgcgac
    31441 agcccatatt cttttgattt ttgctttcat caacagcttg acaataggat tcaataaata
    31501 gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg tcccgatcgg
    31561 ttcgaaaata aagtttattt ctgatgaaat catgatcaat gcagctatga tcaataattc
    31621 aagcgaccaa aacattagtt tagactgtct aagtttttca aacaatagat ttcctccttt
    31681 ctttcttact agtatatatt atattcgtta aaaattcttt tattcttttt ttatttttga
    31741 aaacttgcca tactttttat ttgttttatt ttccagtata ctatacgaaa ggaaggtggc
    31801 tagaccatga aagttgtaca aacaaaagat acaatgagca atatttactt agatgctgta
    31861 cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg agaaatagac
    31921 aaagatgaag cccattgtat ccacttcgtc ctgtactcag ataaaaaaga accgcaagga
    31981 actgtacgtc ttttgccttt agaaaacgga aaaatgaaat tacagcggat ggcgattctt
    32041 tctgaataca gacaccaagg gttaggaaaa atattgattg aagaagcaga aaactttgct
    32101 aagaatcaag gctataatac gattttatta ggggcacagt ccacagctga aactttttac
    32161 gaaaaactag gatatacagc ttacggcgat ccttttgaag atgctggcat gcctcatatt
    32221 tatatgaaaa agactttata aaaatttatt tcgaaaaaag caaaaaacag caagcttttg
    32281 agaaaaatct ctcactggct tactgttctt ttttgtgttt acttacgcga caattgcttg
    32341 taacggttat accaaatatc aatatagcct tccgagaacg ggccatctcc attattgatc
    32401 cagtcaacca gaatcttcac attttctttt aggatgaagt caatatcgtt cgggtaattc
    32461 atcattttgc tgtgcatctc atattcatcg acatccaata gtcgtttctc gccgtcagga
    32521 aataccttga tatccaagtc atagtcgata tatttcaagg cctcatcgtc cagcaaaaaa
    32581 ggtgaagcta gattacaata ataggaaacc cccttttctc tgatcattgc tattacattg
    32641 aaccagtatt ttttatgaaa ataaacaatc gctggttcgc gcgttaccca tcggcgtcca
    32701 tcagattcag tgactaatgt gtgatcattc acgccgatca tagagtgttc gcttgttttt
    32761 aataccatgg tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta actttggatc
    32821 gtcacaaact ctccttcttt tggaactccc atagcaaccc ttccttaagc agcattttgt
    32881 gctttgtgtt tgggtgttca ttacaccgac atcataatta ttatatcaat aaaaatgagc
    32941 tttgcctaga aaggattcct attgtttcga tttttctcta actgatctac cagtttcatt
    33001 tggaccttag gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt cgaagttttg
    33061 ttctcggtaa attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag gagaacatgc
    33121 catttcagat gactaaaaat atgagtaact tctcctaaat gtcgtgtctg ccaaacaaag
    33181 agttcctgct tttcaaatgg gagattttgc tcttcttctg ctactaggtc atcaaacaga
    33241 tcgatttctg gctttgtttc ccattctttt ttcaatcgct catactcctc ttgtgtcact
    33301 tccatcattg gaaatgtcca catattcgcc agcaattttt ggctatctct ttcttcaaaa
    33361 taatacgctc cagaatgatt ttgaagagct gcactgatat aataaacgtc ttttggcttt
    33421 gctttttttg tttttacagg aaaactcgtt tggattcctc gtttgttagc taaacaaaag
    33481 gcttggatcg gacaagtctc acactttgga gaagttggtg tacagatagc tgaacctaga
    33541 tccatcattg cttggttgaa ttcacctgga tgcttttcat caatgatttt ccgcatcgct
    33601 tcatcaaaga ttttcctgct agaagctttt gcgatgtctg cttcaatgca aaacaatctg
    33661 ctgactactc gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa tgcgatactt
    33721 gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg agctaatttc ttcaggcgtt
    33781 tgcggcattt ccccatcaaa ttctgacatg atctgtttag cagcagcttg gatgttccgg
    33841 gctcttgagt aataaccaag gccctcccat gcttttaaaa gcttttcttc tggagcattt
    33901 gctagctctt caattgtagg aaaccattcc atgaaacgat aaaaataatc gatcactgtg
    33961 tctactctgg tttgctgaag catgatctca gagatccaga tacgataagg atcacgatta
    34021 tagcgccaag gcaggtttct tttttcttgt tcataccatt gaataaactg atcttgaaat
    34081 tcttttgttt ctttatctgt ccatttttcc cattctttat tcactttcct cttccttttc
    34141 cgtaatgaat cgctggatca tatccaaatc aaatcctttt tgatataagc tttgctttac
    34201 ttttagattt ctttttttag gttcaaacct taaatttctt cgccatagtt tatctccttg
    34261 tttgactaaa taatcatatt ctatttcttg atctgcttct tgcggaagat cagctatcgc
    34321 ttgttggatc acttcttgag aaaagccttt cgtcatcaat gtttgttgga tttttctcaa
    34381 taattctttt tggctttttc cgtgattttt tccaaacgct ttttcggctg tctgatgtgc
    34441 attttgaact tggtcttcaa atgtatattg gctgagcgct tgttcaatga tttcaggttt
    34501 caaacctttt tgctgcatct gctgcgctaa tttctttggt cctttgtcac ttaagcgcat
    34561 attcgtgcgc acataacttt ccccatagac caaatcatcg acaaggtcca attcttttaa
    34621 gcgggctacg attttatttc gatcttcttg aaggatttcc ttttctttta agtatgttcg
    34681 gatttctttt tccgatcgca actgataact taagtaattc attgctaggt gaaaaccgaa
    34741 atcagctccg ctactcttct tgatctcttg gatagtttct tcgtctaatt cccgtccttt
    34801 taataatcga tagcgtacta atacatcttc agatacttgt aaagcttctc cttcagataa
    34861 atctacgcga tagaaagggc ctttgccttt actgattctc gtaacggtta acataaacat
    34921 cctcctcgaa catatattct ctatcatacc acagaaaatc cccgtaatca ggtattatat
    34981 gttagaataa accacagaaa aaagaaaaag ggagatactt atggcagaaa caattgtcaa
    35041 aactggacaa acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg aaggaatcgg
    35101 ttatttcaaa cggttgatca tatttgttcc atatgcacta ccaaaagaag aagtactagt
    35161 gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa ttaataaaag tcaaaaaaac
    35221 aagcaaagat cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg gctgtcaatt
    35281 acaacactta gcttatcaag ctcaattgga ttttaaaaag gatctgcttt tgcaagcatt
    35341 ggaaaaattc aaaccagcag gctttcgatc ttatcaactt ttgccgacga tcgggatgga
    35401 tgagccttgg cattatagaa acaaagcaca atttcagtta cgaaaaaata agcagactca
    35461 aaaaattgaa gctggtttat acgaagcaaa ctctcatcag ttagtcccta taacagactg
    35521 cttggttcaa gaacctcgaa cacaaaaagt aatgaatact gttgttcaac tattgaataa
    35581 atttgatgca cccatctatg atgaacggac aaacagtgga atcttccgca caatcatggt
    35641 tagaatcggt gttaaaactg gggagttaca agttgttttt attactcgca gtaaaaaatt
    35701 cccgcaaaaa aacgcaatga tccgtgagat caatgaacga ctgcccgaag ttgtttcaat
    35761 catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa cgatccactt
    35821 atggggaaaa gaagccattg aagaacaaat caatgaagtg actttcgatc tttctcctcg
    35881 tgccttcttt caactcaatc ctcagcagac cgaattgctt tatggcgaag gaatcaaagc
    35941 attagatgtc cagcctaatg aaacgatcgt ggatgcttat tgcggagttg ggacgatcgg
    36001 cttaagtgta gcaaaacaag ccaaagaagt acggggaatg gataccgttc ctcaagcaat
    36061 cgctgatgca aaaaaaaatg cggaaagatt aggatatcac aacactcgat atgaagtcgg
    36121 gacagcagag caagttctgc ctaaatggtt gaatgaaggt tttcagcctg atggtatcat
    36181 cgtagatcca ccacgtacgg gtttggataa cgcattgatc caatcattat tgaaataccc
    36241 tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca cttgccagag atctggttaa
    36301 tttaagtaaa aagttcaaga ttgaatattt acagtccgtc gacatgtttc ctcagactgc
    36361 acgttgtgaa gttgtcgtga agatgacgag actcgctgac taaatgaaag aggttgtgaa
    36421 aaagtctgcc ctgtatcaag taataagaac agccaaacaa aaatagctct tcatgtttta
    36481 tgaatactgt acttgtgacc gaagagttaa caggaggaca ctatttattt aaaaaaaggg
    36541 ttgtgacata gtttttgtca cagccctttt tgattattat tggatattca cgtctatcac
    36601 ttgataaaat gcatttgtcg tatcgctgat attccatact gctaaaatca cattatatcc
    36661 ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa cagttgcttt
    36721 gtcgtctatt tttgtgatca attcaaaatc actaaatttc aaaggttgat taggattcca
    36781 agttggtttg gtaatgtagt aatcccaagt acttgtccga tgttgtgctg ttaaattcca
    36841 agtaatattt aatgttcctg gattaaccat gtttttatgc catctgttag atgtctgttc
    36901 atctaaaggc tcaaagccgc tgactcctgc actagcaatt tttccatcaa tgaatgtatt
    36961 tttggtagct tcgatacttt gcggttccca ttgtgctctt ccgacatttt gatttaagtt
    37021 tcctgctgct gatgttccta ggtatgcacg gcttcctgga gaagtaatat aaccatgcgc
    37081 aaaacctgga atgctaaaac cgaacaaccc taaactaact aacccaataa acaataacgt
    37141 actaatcttt ttcatgtaga aacctccttc atttgataac cacattgtaa acgctttatt
    37201 gtattaacac aaacattatg cttttataat tttatgttga agtaaaaaag gtgaaaaaag
    37261 taagatgcag attataacag aagtagacga gaaaagtaca aatagaaaac atgagaatca
    37321 aatcatatga tctttttcaa aaacatcatt catataaaca aatgttcaac tttttaataa
    37381 caacaattat caattttttt cttacttcac aaactaaata caggtcttgc tgtgagcctt
    37441 catggtaaac taatattata gaaaggagtt agtaaaatga agatgataaa agatattcgt
    37501 ttttgggttt gtgttatagg aattgtcata ttaggctttt tatcagggtt gcttagcgga
    37561 aatcccggaa aatattacta ttctttgcaa cttccaccat ttgcaccacc tagctggata
    37621 tttgggccga tgtggacctt actgtatata ttgatgggta tttctcttta tttgttactt
    37681 aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat tcagttcatt
    37741 tttaatttta tatggtctgc tctatttttt aatttaagaa atattttcat tgcagcaatc
    37801 gatattacac ttttagttat tttcttgtca gtgcttatgt atcagttgtg gctgcatcat
    37861 cgcctgacaa tgtggctcat gattccttat tatttatggg tactattcgc aactctattg
    37921 aattattcaa tttatttttt aaactaagcg ctaaaaagag acgtcctgta atttaacaca
    37981 tagaaaaagt gccacctaag ttagattttt tgtctaattt aggtggcact ctttattaac
    38041 caaactattt ttttctagta cccacttgtt gttccatact cattattctg gtcataacca
    38101 taaccatttt gttcctgtcc agcgtaagga tcataacttt gagtatcttg acttggtgta
    38161 tagtactgac ttgtatcttc tgtttgctga taataggtat tcggatcttg gtactgatca
    38221 tatgtgtttt ctgaatagga agaataatcg gtgtaccctt cactattcga tccatcaccg
    38281 tttgtcgaat cgccatcttc cgaatcatca aagaacatca ttgaagcatt ctcatttttc
    38341 aaattaggta atgtatcgct tgtttttata tttaattgtt tcttcagttc attttggatc
    38401 gaaagaagat cattttttcc taaaatttga tagtagatat cattgatcat ttgtccttcg
    38461 ccttgcaact ggtcttgttt gattgtttca aacgccgggg tgtagttcgt cgctacatca
    38521 agcatgtcat cccaatctaa gtccgtcgtc acattgttct caactgcttt taaaattttt
    38581 ctataattgc ttacgccatc taaacttaaa actttattga cgattttcgt cactacttcc
    38641 cgctgacgac gttgtcgacc gatatcgcct tctggatctt cgtgtcgcat tcgagaatag
    38701 gctaaacctt tttctccatt caatgtttgt tcacctactg gtacatggac accatccaaa
    38761 gtaaagtcaa ttttattatt cactttgatg ccaccgaccg cattgatcaa atcttccatg
    38821 ccatccaaat tgatcgttac ataatgatca ataggaatat cgagcagttg ctcaattgaa
    38881 tccatggcca tttcgacacc accaaatgcg taggcatgat ttaatttatc taccgttcca
    38941 taacctacga tattcgtata aatatctcgg tccaaactga tgatcgttga ttttttttgt
    39001 ttcggattaa ctgttactac catcatcgta tcagaacgtc cttgttccgt tcttcctaat
    39061 ccaccggtat ctaaacctaa tagcaaaacc gaaaaaggtt ctttgtcatc aatgcttact
    39121 ctctcagaac gtttggagga tattcgatta gagtttttag aaattttgtt tactgtttgg
    39181 ctcgcttctc ccataaatct gattccgtat gctgataatc cggcaatcag caaaacgaat
    39241 acccctaaca aggttaatag aactttttgc catcgcttca taatacacct ctgtttcaca
    39301 ttttctaaaa tattgtctca tataactgtt caatcagcaa attcactttt taatcataag
    39361 aaaactttaa aaaacatttt tgtgtaacga ttattatttt ttaaaatttc tcaaaattct
    39421 taactatcat accataccgt gaaaaagatc gaaagaccga atacacgcag ttcgcaatcg
    39481 ttttatcttt ttttatgtat tttttccaga aaaagcgaac aacaaggtat tttccaaata
    39541 atcgtgcagt aaaatgatcg cacatacctt tcccatcttt ttaacagtct ttgcctaccg
    39601 ttcagattat cattcgattt cacgacaatt tcttcctatg actggtcctt ttgttaaacc
    39661 catcttctat tcgttttatt tccagctcac tgcatggctc ttaaaatgac tcgaactagt
    39721 gattgcatta ccatcatctt ttaatggata gatatgctcc tcttcaaagt aaaacgattt
    39781 tcttttaaag taatagaatt gacatggtaa tgagtatgta tactagtggt ttcacaaatg
    39841 gtcttttatt caacatccaa aaactgaaat ttctttaaaa tccattacta acgaagaaaa
    39901 tgttccttgc ttgataatta gttaggacat gtcaagtgaa caaatgcatt tacttagcca
    39961 aaaaatctaa aaacttcata aatagacaaa aagacacgac tctctaagaa gtcgtgtctt
    40021 tattcaacaa attattataa tttagtcact tcagcagctt gaggtccgcg tgcaccttca
    40081 acaatcgtga attctacttc ttgaccttct tctaggctct tgaagccgtc tccttgaatt
    40141 gctgagaaat gaacaaatac gtcatctccg ccgtctactg taataaaccc aaatcctttt
    40201 tcattgttga accatttaac cgttccgtgt tccataaatg tatatcctcc aaaaaagttc
    40261 ttgcaagtaa ccttgcaaga acattatacc caatttagaa aacgctagca agctttcgct
    40321 cttttatttt ccaaattatt tttaagactg atatgacgga ggaaatggtt attttgctgc
    40381 ttcgaaacgt ttgtttactt catcccaatt aataacattc cagaatgctg caatataatc
    40441 tggtcggaca tttttatatt tcaaataata agcatgttcc caaacgtcca aacctagaat
    40501 tggtgtcttt ccttccatca atggagaatc ttgatttgca gtagaggtaa tagctaattt
    40561 tccattttcc attacaagcc aagcccatcc agaaccaaat cgtccagcgg ctgctttttt
    40621 gaattcttct ttaaaagaag aaaaatcacc aaaagcttca ttaatcgctt cttttatttc
    40681 tcctgtaggt tcgccaccag catttggtgc catgatttcc cagaaaaatg aatggttagc
    40741 atgtccgcca ccattgttac gtacagctgt cttgatatct gttggaatag cgtccatatc
    40801 agataataat tcttctattg ttttttcgcc taattctggg tatttctcaa ttgctgaatt
    40861 taaattcgtc acataagtat tgtgatgctt atcatgatgc agatgcatcg tttcttcgtc
    40921 aatataaggt tccaatgcgt catatgcata aggtagatct ggtaatgtgt aagtcataaa
    40981 aaaattcctc ctcaatgttt tcttaataat gtctacatta caaagagtaa caaagagaag
    41041 gaaattcttc aattgttatg cctagtcgtc tttttcactt tgacctactt cttcttcaaa
    41101 aacctcagtt tctttaaaaa taaacaactt gctgaaaata tagttcgcaa ggataacaat
    41161 aatttgagtg atgattttcg ccacatactc attgctgttc ataacattga tcaacaatac
    41221 cattgccccc atgtccaatc cgtaagaaag gatacggtag aagaagaact taccgcattc
    41281 aataagtgca tgtgtaaaat tcggtgattt tgaatggaat acccaaagtt tgttcgttac
    41341 aaaagcaaac aatacagaac aaaaccacga aatagaattg gataaggcca tacttaaatc
    41401 aaaatattgg cgtgcgagag caaaactaac aaaatttaca atcgtagcca aaccaccaaa
    41461 aaagagatag actagtatct cccaatatcc ttttgcttct aaatatcctt ttaactgtcg
    41521 atactgcttc atgagccaac ctaccttatt aaaaattctt atttatcata ctctatattt
    41581 caataacttt catctttatt taaaaaaatt aatttttttt gatcctttgc ctttgtctct
    41641 gcttggatcg tcacatgtgt aataccatat tttaatttta ataaagaaat tatacgagga
    41701 tatatctttt caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata
    41761 attatctttt cgctatactg ccaaatatgg acatgatgaa catcctttac cccatcgatt
    41821 gcttcaattt cttgttccat tttttcatag tcgatagcag gtgcactttg tatcaaaata
    41881 gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt
    41941 gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc
    42001 acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa
    42061 ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata
    42121 actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat
    42181 gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc
    42241 aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt
    42301 ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct
    42361 gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc
    42421 gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact
    42481 attccttcct ccattttatt tatattggat agtctttaga caagaataac atttttttga
    42541 tatttcacca atcattcagc tgtttcctta aagcacttgt atttcacttg atacttcgct
    42601 agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt
    42661 atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa
    42721 gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt
    42781 tttcaagtct taaacaacat tcaattggat gggcaaaaga ttgctactcg cattcctgat
    42841 tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca
    42901 aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact
    42961 gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt
    43021 cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc
    43081 aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct
    43141 ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat
    43201 ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta
    43261 acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact
    43321 ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct
    43381 tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat
    43441 taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag gttgtgacat
    43501 ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg
    43561 cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact
    43621 atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc
    43681 catactatag cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat
    43741 gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc
    43801 ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca
    43861 aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat
    43921 gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt
    43981 gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta
    44041 atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg
    44101 aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct
    44161 ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta
    44221 acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct
    44281 gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta
    44341 aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa
    44401 gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca
    44461 cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc
    44521 acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca
    44581 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
    44641 gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat
    44701 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tccatttcca
    44761 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
    44821 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
    44881 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
    44941 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
    45001 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
    45061 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
    45121 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
    45181 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
    45241 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
    45301 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
    45361 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
    45421 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
    45481 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
    45541 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
    45601 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
    45661 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
    45721 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
    45781 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
    45841 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
    45901 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
    45961 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
    46021 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
    46081 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
    46141 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
    46201 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
    46261 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
    46321 tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat
    46381 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
    46441 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
    46501 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
    46561 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
    46621 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
    46681 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
    46741 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
    46801 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
    46861 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
    46921 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
    46981 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
    47041 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
    47101 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
    47161 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
    47221 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
    47281 acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc
    47341 agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat
    47401 agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt
    47461 cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc
    47521 tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa
    47581 attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta
    47641 cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat
    47701 catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga
    47761 tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct
    47821 gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt
    47881 tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac
    47941 caataaataa aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa
    48001 aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt
    48061 tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat
    48121 tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac
    48181 gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat
    48241 ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac
    48301 acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt
    48361 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa
    48421 tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg
    48481 aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt
    48541 ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta
    48601 caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc
    48661 cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc
    48721 cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt
    48781 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
    48841 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
    48901 tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca
    48961 atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt
    49021 cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
    49081 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
    49141 gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt
    49201 catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa
    49261 gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt
    49321 cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat
    49381 ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt
    49441 caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt
    49501 aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga
    49561 atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct
    49621 tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg
    49681 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact
    49741 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
    49801 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
    49861 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga
    49921 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
    49981 taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc
    50041 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
    50101 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
    50161 ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg aattggcgtg
    50221 aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatt
//
LOCUS       DACCIQ010000012        49627 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCIQ010000012 DACCIQ010000000
VERSION     DACCIQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 49627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.012"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            293..1432
                     /locus_tag="IVM62_000694"
     CDS             293..1432
                     /locus_tag="IVM62_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP6844678.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1499..2074)
                     /locus_tag="IVM62_000695"
     CDS             complement(1499..2074)
                     /locus_tag="IVM62_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6844679.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2079..3053)
                     /locus_tag="IVM62_000696"
     CDS             complement(2079..3053)
                     /locus_tag="IVM62_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP6844680.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3077..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3336..4303
                     /locus_tag="IVM62_000697"
                     /pseudo
     CDS             3336..4303
                     /locus_tag="IVM62_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            4416..4694
                     /locus_tag="IVM62_000698"
     CDS             4416..4694
                     /locus_tag="IVM62_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6844681.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IVM62_000699"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IVM62_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP6844682.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IVM62_000700"
     CDS             5160..5906
                     /locus_tag="IVM62_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6844683.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IVM62_000701"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IVM62_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6844684.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IVM62_000702"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IVM62_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6844685.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IVM62_000703"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IVM62_000703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP6844686.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IVM62_000704"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IVM62_000704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP6844687.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IVM62_000705"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IVM62_000705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6844688.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IVM62_000706"
     CDS             10643..11185
                     /locus_tag="IVM62_000706"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6844689.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IVM62_000707"
     CDS             11208..12764
                     /locus_tag="IVM62_000707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6844690.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IVM62_000708"
     CDS             12778..13680
                     /locus_tag="IVM62_000708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP6844691.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IVM62_000709"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IVM62_000709"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP6844692.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IVM62_000710"
     CDS             15123..15545
                     /locus_tag="IVM62_000710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP6844693.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IVM62_000711"
     CDS             16014..16244
                     /locus_tag="IVM62_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP6844694.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IVM62_000712"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IVM62_000712"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6844695.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IVM62_000713"
     CDS             17655..17831
                     /locus_tag="IVM62_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6844696.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IVM62_000714"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IVM62_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP6844697.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18793
                     /locus_tag="IVM62_000715"
     CDS             18326..18793
                     /locus_tag="IVM62_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844698.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18878..19762
                     /locus_tag="IVM62_000716"
     CDS             18878..19762
                     /locus_tag="IVM62_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6844699.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IVM62_000717"
     CDS             19879..20112
                     /locus_tag="IVM62_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP6844700.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IVM62_000718"
     tRNA            20200..20280
                     /locus_tag="IVM62_000718"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IVM62_000719"
     tRNA            20287..20358
                     /locus_tag="IVM62_000719"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20873..21580
                     /locus_tag="IVM62_000720"
     CDS             20873..21580
                     /locus_tag="IVM62_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6844701.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21639..22273)
                     /locus_tag="IVM62_000721"
                     /pseudo
     CDS             complement(21639..22273)
                     /locus_tag="IVM62_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22400..22687
                     /locus_tag="IVM62_000722"
     CDS             22400..22687
                     /locus_tag="IVM62_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844702.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22795..23115
                     /locus_tag="IVM62_000723"
     CDS             22795..23115
                     /locus_tag="IVM62_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6844703.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23196..24509
                     /locus_tag="IVM62_000724"
     CDS             23196..24509
                     /locus_tag="IVM62_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6844704.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24506..25225
                     /locus_tag="IVM62_000725"
     CDS             24506..25225
                     /locus_tag="IVM62_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP6844705.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25353..25904
                     /gene="raiA"
                     /locus_tag="IVM62_000726"
     CDS             25353..25904
                     /gene="raiA"
                     /locus_tag="IVM62_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP6844706.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26126..28660
                     /gene="secA"
                     /locus_tag="IVM62_000727"
     CDS             26126..28660
                     /gene="secA"
                     /locus_tag="IVM62_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP6844707.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28786..29887
                     /gene="prfB"
                     /locus_tag="IVM62_000728"
     CDS             join(28786..28848,28850..29887)
                     /gene="prfB"
                     /locus_tag="IVM62_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP6844708.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29992..30678
                     /gene="ftsE"
                     /locus_tag="IVM62_000729"
     CDS             29992..30678
                     /gene="ftsE"
                     /locus_tag="IVM62_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP6844709.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30662..31555
                     /locus_tag="IVM62_000730"
     CDS             30662..31555
                     /locus_tag="IVM62_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844710.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31707..33155
                     /gene="cls"
                     /locus_tag="IVM62_000731"
     CDS             31707..33155
                     /gene="cls"
                     /locus_tag="IVM62_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6844711.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            33492..34349
                     /locus_tag="IVM62_000732"
     CDS             33492..34349
                     /locus_tag="IVM62_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP6844712.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            34464..35384
                     /gene="pstC"
                     /locus_tag="IVM62_000733"
     CDS             34464..35384
                     /gene="pstC"
                     /locus_tag="IVM62_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP6844713.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            35384..36268
                     /gene="pstA"
                     /locus_tag="IVM62_000734"
     CDS             35384..36268
                     /gene="pstA"
                     /locus_tag="IVM62_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP6844714.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            36283..37089
                     /gene="pstB"
                     /locus_tag="IVM62_000735"
     CDS             36283..37089
                     /gene="pstB"
                     /locus_tag="IVM62_000735"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6844715.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            37105..37863
                     /locus_tag="IVM62_000736"
     CDS             37105..37863
                     /locus_tag="IVM62_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6844716.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            37876..38553
                     /gene="phoU"
                     /locus_tag="IVM62_000737"
     CDS             37876..38553
                     /gene="phoU"
                     /locus_tag="IVM62_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP6844717.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            38804..39822
                     /locus_tag="IVM62_000738"
                     /pseudo
     CDS             38804..39822
                     /locus_tag="IVM62_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            39819..40586
                     /locus_tag="IVM62_000739"
     CDS             39819..40586
                     /locus_tag="IVM62_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6844718.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            40686..42254
                     /gene="liaX"
                     /locus_tag="IVM62_000740"
     CDS             40686..42254
                     /gene="liaX"
                     /locus_tag="IVM62_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP6844719.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            42554..42871
                     /locus_tag="IVM62_000741"
     CDS             42554..42871
                     /locus_tag="IVM62_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP6844720.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            42871..43221
                     /locus_tag="IVM62_000742"
     CDS             42871..43221
                     /locus_tag="IVM62_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6844721.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            43388..44326
                     /locus_tag="IVM62_000743"
     CDS             43388..44326
                     /locus_tag="IVM62_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6844722.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            44341..45171
                     /locus_tag="IVM62_000744"
     CDS             44341..45171
                     /locus_tag="IVM62_000744"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6844723.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            45215..46240
                     /locus_tag="IVM62_000745"
     CDS             45215..46240
                     /locus_tag="IVM62_000745"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6844724.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            46255..47193
                     /gene="galU"
                     /locus_tag="IVM62_000746"
     CDS             46255..47193
                     /gene="galU"
                     /locus_tag="IVM62_000746"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6844725.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            47318..47758
                     /locus_tag="IVM62_000747"
     CDS             47318..47758
                     /locus_tag="IVM62_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP6844726.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            47761..48363
                     /locus_tag="IVM62_000748"
     CDS             47761..48363
                     /locus_tag="IVM62_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6844727.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(48422..49528)
                     /locus_tag="IVM62_000749"
     CDS             complement(48422..49528)
                     /locus_tag="IVM62_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6844728.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT        16367 a         8647 c        10752 g        13861 t
ORIGIN      
        1 gtttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
       61 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
      121 agtagaatag gaatctatgg gcaagtcctc tcgtataaat aaaataggag ggaagtagca
      181 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
      241 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
      301 agaagaaaaa atcgcgattt tacaagagat tatccgaatt aaatcagtga atggaaacga
      361 aggtgaagta gcagcctatt tgaataagct tttggcaagg catgacataa caggagagat
      421 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
      481 aaaagtgtta ggcctatctg gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg
      541 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
      601 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
      661 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
      721 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
      781 agagccaacc aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
      841 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
      901 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
      961 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
     1021 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
     1081 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
     1141 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
     1201 tcctttgatt cattccattc agcaacaatt cagccagcca ttacctttag ttggagcggc
     1261 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
     1321 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
     1381 tttggatatg atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
     1441 taataaatga aaaaacagag ccagattagc attattttgc taatccagct ctgtttattc
     1501 agcctatttt tctttttgaa tatatcaagc gttggggcaa catagtgatg actaatgaaa
     1561 caacgacgat agacaacaag cgatcaaggt agtctgtacc cacctgtacc aagatcacac
     1621 ttgttgtctg acttaaacct aacccatgta atagctgaac gatcatactg gatcctgagg
     1681 aggtgatccc gccaaataac tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg
     1741 acaaaatcaa agcgagtaat aacagtttca agctgttttt atctttgccg agatatcttg
     1801 gcaaaatcaa tccagcaagc aagcctgtga gaatttggat tggcgaataa tacagagaaa
     1861 aaatatctgt agtcactcca cttaaaatac cgctggacaa tcctgtcagc attccaggta
     1921 caggcccaag caatgcacca gcaaaaatcg ttccgataga atcgagatag attggcaaac
     1981 gaagccataa agcaatcgtt cctccaatat aattgatccc aatacacaaa gccatcactg
     2041 taagtacgcg agttgccatt ttgtttttat tcattttgtt agcccatctt taacctttcc
     2101 aaatctttca taatcgtttc ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa
     2161 aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg ccaaaattca
     2221 taacgatcaa ccaatgtttg tccgcgactt attccttgag tttctacagc agtgaagctt
     2281 ttaaagcctt cacagatgga ctcatcgata aaataagcaa cagctaatgg atcattgatg
     2341 acacatccta agattctttc ttgtttccag tgaaagtcaa aataaaaacg agtgatcgct
     2401 tttagatact ctccctcttc aggattcatt tggcagcaat attcaaggat agtcggtgtc
     2461 aagacgattt ctcttgtcac atctaaccca accatttcta tcggttgttt gagcttttcg
     2521 aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac tggtgaacag
     2581 ttaccatggc ttttgtaagt tccgcccatt gagacaaaac gatccatatg tttcccaaca
     2641 gatgggctgt ttttcaaagc agaagcgata ttggtcaaag gccccaaagc aatgactgat
     2701 gtatctgttt tttctttgaa ataatcagtc agaaaatcaa ctgcatggag cgattctgcc
     2761 tgtttggcta agaccatcgg gaaatttgtg tcccctaatc catccaaccc atgagtgtct
     2821 tgcgcactaa tgaaaggttg cttcaaaggt ttttccattc cttgataaac agggatatct
     2881 aaacgatcac agcgctccaa acatttcagc acattctgcg taccgatatc tacagggaca
     2941 ttcccgcaaa cgacggtaat agcaactact tcgatatcag gggatttgac agctagtgat
     3001 aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttactttacg cattatttcc
     3061 aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga accagtccaa
     3121 gacgagtgtc ttgtttataa tgcacgaaat tagtatactg gcttttattg tgaatgtaaa
     3181 tgctctctta caagtaaatc tattgagaag aactttaagt aaatgataca atgaaaaaga
     3241 aatggaattt acgtgtgccg aagaaaaaga atcctattga tcggtatatt gtataataaa
     3301 tagattatag tttggcagaa agcggcgggt gtacgatggt tattttttat ttcattctta
     3361 ttctgatatt tataggaatc agttaccatt ttctttctta tttttatgca aagaaattag
     3421 taagagcggg ggtacagaga ggctatgact ggtatgaaga aactgggcac cgcttgatgg
     3481 atcctgctgc agtcacgtca gtagaaaaaa agcgacaaaa attagaagaa gagtgtgaaa
     3541 gcttctggga acaaggaata ccgaaaacga tcaaaagtta tgacgggttg aaactagctg
     3601 gacagatgtt cctccaacca actcagcaga ataaatgggt catttgtgta catgattatc
     3661 gaagtacagg gaaaagagat atgtctcata taggtaaaag atatgcagag aaaggattca
     3721 acgttcttat acccgattta cgtgcgcatg gagaaagtga aggagaaatc atcggaatgg
     3781 gatggctaga ccggctagat ttaattgcat ggatccaact catcttagat gaacaaccag
     3841 atcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaaaaaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaattg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga gagctctttt
    20401 ttttgctttg ctaccttgtt ccttgaagat tctgctgaat taagatagag ttaagttaac
    20461 tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa
    20521 gttatatgat ttggttaaat cagaaaaata caatattgaa gtgaatattc cgaaaaaagg
    20581 cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa aatggatggc
    20641 taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga ttttagggtc
    20701 ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata ggagtaggga
    20761 gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt ttcagttgtg
    20821 aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa
    20881 tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg aacaaggtat
    20941 atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat tcaatgtcag
    21001 ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct tagaaagaaa
    21061 gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac
    21121 aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga caatctctta
    21181 tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta aagatgacac
    21241 gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc
    21301 cagtgttcca caaaagctga tcgatggcta cagcaaagca gagattactg aatcgttgta
    21361 tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc
    21421 tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag atgcagttct
    21481 tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg tgcgtacgca
    21541 atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg atccctgatt
    21601 ccttattaga aataaaagag aatcagaatc acctgttttt atatatgatc agttatcagt
    21661 tgctcgtgat aagaaacaga accctcttca aattggactt gtccgttcag cagctcaatg
    21721 atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga
    21781 tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt
    21841 cctagttgcg gatacgtgag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
    21901 acaatgcttg agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
    21961 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
    22021 cttccaacat cggtacgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga
    22081 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
    22141 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
    22201 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
    22261 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
    22321 ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca
    22381 aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
    22441 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
    22501 gtttcccagt aacgggcttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
    22561 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
    22621 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
    22681 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
    22741 taacagacgc gaagaaaacg tttgcatagt ttgttttaaa aaggagagaa aagtatggct
    22801 aaaaaaacaa ttatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
    22861 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
    22921 gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt
    22981 cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc
    23041 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
    23101 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
    23161 cttgtttttt cgtatatttt tttagggcga agaatatgaa tcatttttgg ggtagaaggc
    23221 ttctgacgag agatattgaa gcaatagact gtgaggaggt aaatttacca aattatgaga
    23281 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
    23341 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
    23401 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
    23461 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga
    23521 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
    23581 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
    23641 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
    23701 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
    23761 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
    23821 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
    23881 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
    23941 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt
    24001 tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
    24061 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
    24121 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
    24181 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
    24241 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
    24301 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
    24361 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
    24421 actttttcta tgaagaaaaa actgcagcta ttcgaaaagt ttgtcaggaa atcaaagaaa
    24481 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcttcca
    24541 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
    24601 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
    24661 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
    24721 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
    24781 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
    24841 actagcaggc acctttgcat atagatggac gcagataaaa aaagaatacc cgacatacat
    24901 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
    24961 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
    25021 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
    25081 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
    25141 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
    25201 ccgaaccatt tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc
    25261 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
    25321 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
    25381 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
    25441 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
    25501 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
    25561 cttctccaga tttatacgca agtatcgatt tggttgttga taaattggaa cgccaaatcc
    25621 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
    25681 ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
    25741 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
    25801 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    25861 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    25921 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    25981 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    26041 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    26101 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    26161 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    26221 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    26281 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    26341 agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct
    26401 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    26461 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    26521 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    26581 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    26641 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    26701 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    26761 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    26821 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    26881 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    26941 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    27001 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    27061 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    27121 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    27181 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    27241 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccaaat
    27301 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    27361 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    27421 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    27481 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    27541 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    27601 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    27661 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    27721 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    27781 acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    27841 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    27901 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    27961 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    28021 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    28081 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagcattac
    28141 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    28201 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    28261 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    28321 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    28381 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    28441 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    28501 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    28561 aaaacagcca atccatgtga ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    28621 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    28681 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    28741 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    28801 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga
    28861 cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt
    28921 ttgggatgac agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac
    28981 ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat
    29041 gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt
    29101 agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa
    29161 caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag
    29221 catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact
    29281 agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca
    29341 taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc
    29401 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    29461 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    29521 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    29581 gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca
    29641 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    29701 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    29761 tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    29821 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    29881 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    29941 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    30001 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt
    30061 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    30121 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    30181 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    30241 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    30301 atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac
    30361 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    30421 caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa
    30481 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    30541 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    30601 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    30661 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    30721 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt
    30781 cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa
    30841 cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    30901 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    30961 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    31021 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    31081 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    31141 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    31201 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    31261 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    31321 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    31381 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    31441 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    31501 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    31561 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    31621 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    31681 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    31741 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    31801 ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat
    31861 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    31921 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    31981 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    32041 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    32101 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    32161 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    32221 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    32281 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    32341 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    32401 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    32461 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    32521 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    32581 tttttccgtc cattcacact actggtgata tagctgttca aatcgtgaca agcggaccag
    32641 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    32701 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    32761 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    32821 tcgtttactg ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg
    32881 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    32941 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    33001 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    33061 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    33121 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    33181 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    33241 tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa
    33301 agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat
    33361 agaaactttt aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg
    33421 attttgattc gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa
    33481 ggagatttct aatgaaaaaa cttaaattat tcttaccaat aataggatta gccattcttt
    33541 tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg
    33601 ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat
    33661 tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag
    33721 tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat
    33781 tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg
    33841 taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga
    33901 gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa
    33961 cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac
    34021 aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg
    34081 ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga
    34141 cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa
    34201 gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac
    34261 aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg
    34321 attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa
    34381 cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa
    34441 acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa
    34501 aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg
    34561 tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc acctttttta
    34621 ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg
    34681 gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga
    34741 cctttttatc tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg
    34801 agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta
    34861 ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta
    34921 tttttggcgg gacagggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct
    34981 tgccgactgt cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg
    35041 aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag
    35101 cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag
    35161 ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacagga cttactactc
    35221 ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag
    35281 ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta
    35341 atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc
    35401 agataaaatt gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc
    35461 agctttacta ctttatatct tagttcgggg aataccgcat atttcatggt ccttcttgac
    35521 cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat
    35581 ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta
    35641 tctttcagaa tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat
    35701 cttgagctcg ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca
    35761 attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc
    35821 tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc
    35881 aggattggcc ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc
    35941 gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc
    36001 tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc
    36061 attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat
    36121 ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc
    36181 tgccgtgttg atcattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg
    36241 attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg
    36301 aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat
    36361 ctacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa
    36421 aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg
    36481 ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa
    36541 ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg
    36601 gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg
    36661 aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa
    36721 caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct
    36781 ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg
    36841 ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca
    36901 cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca
    36961 gctcgtatca gcgactatac tgcctttttc tatctaggaa aagtaatgga atatgacaaa
    37021 actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat
    37081 tttggttaag acggaggcct caacatgaca aaagaaatta tttcatctaa agatcttcat
    37141 ctgtattatg gaaagaaaga agcactaaaa gggatcaatc taagtttcaa tcaaggggaa
    37201 atcactgcga tgatcggtcc ttctggttgc gggaaatcca cttatctgcg ttctttgaac
    37261 cggatgaatg acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa
    37321 gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc
    37381 cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta
    37441 aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg
    37501 tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg
    37561 cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg
    37621 gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta
    37681 gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt
    37741 atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa cgatacgaag
    37801 aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga
    37861 taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat
    37921 cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac
    37981 atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac
    38041 atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca
    38101 actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc
    38161 gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg
    38221 gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc
    38281 ctagtagctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa
    38341 agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat
    38401 cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc
    38461 ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact
    38521 gagttaaata cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt
    38581 taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag
    38641 aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga
    38701 tgcaagcttt tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc
    38761 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaaattagca tcatcactat
    38821 attttttatt tgtgtgctta cttcatgggt tattcagcat caagctgctt tatatgaagt
    38881 acctgtcatc gagatcgaaa aagtacgaat aaaagaggat catcaattga ttacaggaag
    38941 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
    39001 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
    39061 tgaatggcag ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt
    39121 cattggcgcc atgttgctta ttggcggaaa acagggtcta tttgctttag gaagtctcat
    39181 catcaatatc gttttacttt acttttgttg ctcatcttta ctaaaacaga agggatttct
    39241 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
    39301 aatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
    39361 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
    39421 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
    39481 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
    39541 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
    39601 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
    39661 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
    39721 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
    39781 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
    39841 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
    39901 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
    39961 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
    40021 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
    40081 tttgtttcct actattgctt cctatcactg attttttagc gatccaaggt tttacatcgg
    40141 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
    40201 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgctagt gtagcaattt
    40261 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
    40321 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
    40381 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
    40441 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
    40501 tgtctgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
    40561 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
    40621 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
    40681 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
    40741 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaataaaaa
    40801 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
    40861 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
    40921 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
    40981 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
    41041 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
    41101 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
    41161 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
    41221 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
    41281 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
    41341 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
    41401 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
    41461 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
    41521 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
    41581 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
    41641 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
    41701 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
    41761 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
    41821 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
    41881 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
    41941 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
    42001 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
    42061 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
    42121 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
    42181 cagatggtga aatcacgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
    42241 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
    42301 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag gctatctctt
    42361 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
    42421 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
    42481 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
    42541 gaggtgagac atgatgaaaa aaaagttgac aaaatccaat aaaaatgttg ttttgacagg
    42601 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
    42661 tgtattcctg agcttagttg cttttggaag tccaatcatt ttatacattt tattagcttt
    42721 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
    42781 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
    42841 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
    42901 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
    42961 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
    43021 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
    43081 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
    43141 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
    43201 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
    43261 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
    43321 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
    43381 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
    43441 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
    43501 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
    43561 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
    43621 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cggctccaga
    43681 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
    43741 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
    43801 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
    43861 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggtcatcgat tgatcgcaga
    43921 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
    43981 cctggaacat ctaattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
    44041 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
    44101 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
    44161 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
    44221 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
    44281 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
    44341 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
    44401 gcattgatca tcgtatcagg tatcattatc gccatgtggc ttagcagcag agaggctgtt
    44461 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
    44521 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
    44581 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
    44641 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
    44701 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
    44761 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
    44821 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
    44881 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
    44941 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
    45001 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
    45061 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
    45121 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
    45181 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
    45241 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
    45301 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
    45361 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
    45421 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
    45481 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
    45541 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
    45601 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag cgaaacatga
    45661 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
    45721 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
    45781 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
    45841 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
    45901 agcaatgggt gccaacccgc tgacctttat cggattaagc ggagtaggcg atctagtcgt
    45961 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
    46021 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
    46081 agcagcgatg gaactagccc aaataatgga aatcgaaatg cccatcactc aaacaatcta
    46141 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
    46201 tgggaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
    46261 gtcaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
    46321 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
    46381 gaagcgttga aatcaggaat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
    46441 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
    46501 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
    46561 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
    46621 ccgtttgtcg ttatgctagg cgatgatatc atggaagatg aagtaccatt gacacagcaa
    46681 ttaatcgatg attacgaacg tactcatgct tctacgatcg ccgttatgca agttcctcat
    46741 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
    46801 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
    46861 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
    46921 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
    46981 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
    47041 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtatatc
    47101 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
    47161 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
    47221 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
    47281 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
    47341 attgattgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
    47401 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
    47461 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttgc tggtcaaaag
    47521 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
    47581 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
    47641 gaaagtcggc ggaatcggta aagcgacagc taaagcgacg gttgccggaa aagtcggtgg
    47701 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
    47761 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggaacagc cgcagcaatc
    47821 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
    47881 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
    47941 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
    48001 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
    48061 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
    48121 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
    48181 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
    48241 atgtcagaag caaaaccagt tgcagatgac acaaaagaaa tgatgaaaat ggcaaaagaa
    48301 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
    48361 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
    48421 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
    48481 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
    48541 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
    48601 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
    48661 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
    48721 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtat attttttctt
    48781 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    48841 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgtg tttcctggtg
    48901 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    48961 aaacaccttg ttttttcaat tggtattcga tttctgcaat gatcgcttgt tcagtcacgc
    49021 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    49081 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    49141 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    49201 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    49261 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    49321 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    49381 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    49441 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    49501 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    49561 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    49621 aattttc
//
LOCUS       DACCIQ010000013        48513 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCIQ010000013 DACCIQ010000000
VERSION     DACCIQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 48513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.013"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..623)
                     /locus_tag="IVM62_000750"
     CDS             complement(<1..623)
                     /locus_tag="IVM62_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6844729.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFA"
     gene            complement(772..1485)
                     /locus_tag="IVM62_000751"
     CDS             complement(772..1485)
                     /locus_tag="IVM62_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6844730.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            1660..3084
                     /locus_tag="IVM62_000752"
     CDS             1660..3084
                     /locus_tag="IVM62_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6844731.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            3383..4090
                     /locus_tag="IVM62_000753"
     CDS             3383..4090
                     /locus_tag="IVM62_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6844732.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4077..4400
                     /locus_tag="IVM62_000754"
     CDS             4077..4400
                     /locus_tag="IVM62_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6844733.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(4489..5526)
                     /locus_tag="IVM62_000755"
     CDS             complement(4489..5526)
                     /locus_tag="IVM62_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6844734.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5847..6830)
                     /locus_tag="IVM62_000756"
     CDS             complement(5847..6830)
                     /locus_tag="IVM62_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP6844735.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7245..8015)
                     /locus_tag="IVM62_000757"
     CDS             complement(7245..8015)
                     /locus_tag="IVM62_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844736.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8008..8943)
                     /locus_tag="IVM62_000758"
     CDS             complement(8008..8943)
                     /locus_tag="IVM62_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844737.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9259..10908)
                     /locus_tag="IVM62_000759"
     CDS             complement(9259..10908)
                     /locus_tag="IVM62_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6844738.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11025..13628)
                     /locus_tag="IVM62_000760"
     CDS             complement(11025..13628)
                     /locus_tag="IVM62_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6844739.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(13640..14689)
                     /locus_tag="IVM62_000761"
     CDS             complement(13640..14689)
                     /locus_tag="IVM62_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP6844740.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(14715..15029)
                     /locus_tag="IVM62_000762"
     CDS             complement(14715..15029)
                     /locus_tag="IVM62_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP6844741.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15045..15491)
                     /locus_tag="IVM62_000763"
     CDS             complement(15045..15491)
                     /locus_tag="IVM62_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6844742.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(15492..17468)
                     /locus_tag="IVM62_000764"
     CDS             complement(15492..17468)
                     /locus_tag="IVM62_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP6844743.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(17626..18522)
                     /locus_tag="IVM62_000765"
     CDS             complement(17626..18522)
                     /locus_tag="IVM62_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6844744.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(18515..19195)
                     /locus_tag="IVM62_000766"
     CDS             complement(18515..19195)
                     /locus_tag="IVM62_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6844745.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(19196..19978)
                     /locus_tag="IVM62_000767"
     CDS             complement(19196..19978)
                     /locus_tag="IVM62_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6844746.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19971..20888)
                     /locus_tag="IVM62_000768"
     CDS             complement(19971..20888)
                     /locus_tag="IVM62_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6844747.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21052..21375)
                     /locus_tag="IVM62_000769"
     CDS             complement(21052..21375)
                     /locus_tag="IVM62_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6844748.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            22163..25390
                     /gene="fss3"
                     /locus_tag="IVM62_000770"
     CDS             22163..25390
                     /gene="fss3"
                     /locus_tag="IVM62_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6844749.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            25503..25817
                     /locus_tag="IVM62_000771"
     CDS             25503..25817
                     /locus_tag="IVM62_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6844750.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            25830..26204
                     /locus_tag="IVM62_000772"
     CDS             25830..26204
                     /locus_tag="IVM62_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6844751.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            26214..26549
                     /locus_tag="IVM62_000773"
     CDS             26214..26549
                     /locus_tag="IVM62_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6844752.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            26632..27972
                     /locus_tag="IVM62_000774"
     CDS             26632..27972
                     /locus_tag="IVM62_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6844753.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            28050..28724
                     /locus_tag="IVM62_000775"
     CDS             28050..28724
                     /locus_tag="IVM62_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6844754.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            28957..29391
                     /gene="dcm"
                     /locus_tag="IVM62_000776"
     CDS             28957..29391
                     /gene="dcm"
                     /locus_tag="IVM62_000776"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6844755.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            29392..30099
                     /locus_tag="IVM62_000777"
     CDS             29392..30099
                     /locus_tag="IVM62_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6844756.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            30089..30379
                     /locus_tag="IVM62_000778"
     CDS             30089..30379
                     /locus_tag="IVM62_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844757.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            30638..31822
                     /locus_tag="IVM62_000779"
     CDS             30638..31822
                     /locus_tag="IVM62_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6844758.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            31837..31956
                     /locus_tag="IVM62_000780"
     CDS             31837..31956
                     /locus_tag="IVM62_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6844759.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            32702..34612
                     /gene="ltrA"
                     /locus_tag="IVM62_000781"
     CDS             32702..34612
                     /gene="ltrA"
                     /locus_tag="IVM62_000781"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6844760.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            34716..34940
                     /locus_tag="IVM62_000782"
     CDS             34716..34940
                     /locus_tag="IVM62_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844761.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            34953..35456
                     /locus_tag="IVM62_000783"
     CDS             34953..35456
                     /locus_tag="IVM62_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6844762.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            35516..35905
                     /locus_tag="IVM62_000784"
     CDS             35516..35905
                     /locus_tag="IVM62_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6844763.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            35892..37394
                     /locus_tag="IVM62_000785"
                     /pseudo
     CDS             35892..37394
                     /locus_tag="IVM62_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            38139..39965
                     /gene="ltrA"
                     /locus_tag="IVM62_000786"
     CDS             38139..39965
                     /gene="ltrA"
                     /locus_tag="IVM62_000786"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6844764.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            40000..41019
                     /locus_tag="IVM62_000787"
                     /pseudo
     CDS             40000..41019
                     /locus_tag="IVM62_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303685.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            41024..43147
                     /locus_tag="IVM62_000788"
     CDS             41024..43147
                     /locus_tag="IVM62_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6844765.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            43144..44148
                     /locus_tag="IVM62_000789"
     CDS             43144..44148
                     /locus_tag="IVM62_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6844766.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            44167..45078
                     /locus_tag="IVM62_000790"
     CDS             44167..45078
                     /locus_tag="IVM62_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6844767.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            45265..45521
                     /locus_tag="IVM62_000791"
                     /pseudo
     CDS             45265..45521
                     /locus_tag="IVM62_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(45738..45953)
                     /locus_tag="IVM62_000792"
     CDS             complement(45738..45953)
                     /locus_tag="IVM62_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844768.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(46202..46420)
                     /locus_tag="IVM62_000793"
                     /pseudo
     CDS             complement(46202..46420)
                     /locus_tag="IVM62_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(46715..48154)
                     /locus_tag="IVM62_000794"
     CDS             complement(46715..48154)
                     /locus_tag="IVM62_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6844769.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT        15421 a         9092 c         9374 g        14626 t
ORIGIN      
        1 gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt taaaacagcg
       61 ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag tttaataaat
      121 aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc tcctgtatag
      181 gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg agtaaacatc
      241 atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga
      301 tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac taataatgta
      361 gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta
      421 ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga attaataagt
      481 gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc tcgcaaagtt
      541 cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc aaatacaatg
      601 tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa tcgattactt
      661 tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat taaatatata
      721 tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag cttatttttc
      781 aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg aatactcgaa
      841 aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt cacctggatc
      901 tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac gtatgtgcga
      961 agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt tctttaaatg
     1021 ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg
     1081 ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta cactgatact
     1141 taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga tttctgaatc
     1201 tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt
     1261 atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca aaactccttc
     1321 ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat aaataactgc
     1381 caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta caattttttt
     1441 ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg accctccaat
     1501 caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc acatcaaaat
     1561 tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt aagttgtaag
     1621 cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat gaaaaagcta
     1681 acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa
     1741 ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca accttaccgg
     1801 ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata taaagaagat
     1861 atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc ttgggcacga
     1921 atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa
     1981 ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata tcattttgac
     2041 atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt tgatgcttat
     2101 gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca ttggtttacc
     2161 ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca ttatccaaac
     2221 ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc acacgcaaaa
     2281 gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat tctaaatttg
     2341 agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga aatcgctgat
     2401 ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc taaaaagctt
     2461 atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga agatcttgta
     2521 tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc acgtcgcgtc
     2581 aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg gtttttcgaa
     2641 ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat ttacgaaaaa
     2701 ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga agcatttatc
     2761 tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac agggcaaata
     2821 attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg gaaggcaata
     2881 aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg ctggtcatgg
     2941 atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac tcaaaaaaga
     3001 acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg atttagatac
     3061 gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa cacattagaa
     3121 aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc atttatactt
     3181 gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct ggaaatctaa
     3241 cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa ttgaagttga
     3301 caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg aaaatttatg
     3361 tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg cgatcaaaga
     3421 cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta tcgttgggaa
     3481 agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct atgctggttc
     3541 tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct ccatcgttct
     3601 ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg ctccttattt
     3661 caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag atgaaagttt
     3721 cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc gttggatgaa
     3781 tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg gcgcactttt
     3841 agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt
     3901 tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac gattacagtt
     3961 gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta tcccaagtaa
     4021 cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga tcaaacatgc
     4081 cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact tggctttcta
     4141 ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt
     4201 taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta tttacccaaa
     4261 atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt cctacacttt
     4321 tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt ctttcccttt
     4381 cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc
     4441 tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt atccgttgaa
     4501 cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt tggttgcttg
     4561 tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac tgcggataat
     4621 cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt cgatttcata
     4681 gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt ggtttttcca
     4741 aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc caaatggtcg
     4801 gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag cgacatactc
     4861 tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat aatcgattgg
     4921 atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag tcgcgtagac
     4981 ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt
     5041 gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac caaatttttc
     5101 aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt cttcatctga
     5161 aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt cttgattgct
     5221 tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac
     5281 ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac gtaaagcatt
     5341 tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata ctgcgttgac
     5401 ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat caataaagct
     5461 ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta attccttttc
     5521 tgacatacga attcctcctt agcttcatca agttggagca tccatacagt tttcatttca
     5581 atatatatag tatgctattt gttccacttt tattcatcat aattattctc ttacttaaag
     5641 taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt gtcacaacct
     5701 cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg acaaaatatg
     5761 aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacgacct
     5821 ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg atgttctttt
     5881 tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat caactgaact
     5941 cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg atgtagtcct
     6001 gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg cagacgaagt
     6061 agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc ataaggattc
     6121 ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc caattcttct
     6181 cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg agcgatttgg
     6241 tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt actcatctct
     6301 ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag gtgactgatc
     6361 cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa
     6421 ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag gatcaagcca
     6481 ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat atcgattcct
     6541 tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg cttgtctccc
     6601 caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg
     6661 tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa gaagacatcc
     6721 ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc ttccattggt
     6781 gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat ttatttgtta
     6841 ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt cattatacta
     6901 ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa
     6961 gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt caaaagcttg
     7021 catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt gtttggctgt
     7081 tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg tgttcaatca
     7141 gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt tcccgtttgt
     7201 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact tctcaatcct
     7261 cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt
     7321 acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta acggaaaccg
     7381 gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg cggcaacata
     7441 gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa tgtaggcacg
     7501 atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac
     7561 aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac atgaagtggt
     7621 acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg acccactccg
     7681 ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa gatttcttcg
     7741 atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata cgaactagtg
     7801 atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa accacccatg
     7861 tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga tgtcgtgatc
     7921 acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc cttgatagct
     7981 aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat gattattttc
     8041 ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca tagaaagaac
     8101 tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat ttcgttcaac
     8161 ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa tcacaggttt
     8221 ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg atagaagctc
     8281 tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt tgcgacaaag
     8341 catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa
     8401 ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg ctgttggttc
     8461 atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca gtcttcgctt
     8521 cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat tggactgctt
     8581 caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt agcctgcttg
     8641 attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa
     8701 tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga agacattcac
     8761 ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag acttcccagc
     8821 tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat cgattcctcg
     8881 caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa gcgcatttgc
     8941 catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa tacagcagat
     9001 gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat
     9061 gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat cgaagataaa
     9121 ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga acacttttgt
     9181 ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg atccaataaa
     9241 tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat caaataacgc
     9301 ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc
     9361 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
     9421 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
     9481 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
     9541 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa tctcatctac
     9601 gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga tcgatttgat
     9661 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
     9721 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
     9781 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
     9841 gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta acaatggatg
     9901 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatgggcat
     9961 ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca attcccacag
    10021 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
    10081 tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac tcccactctt
    10141 catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
    10201 ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa tcgttgaaac
    10261 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
    10321 cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc ctaaaagaag
    10381 caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
    10441 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
    10501 acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat aaaaagcaat
    10561 cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc
    10621 ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga acatatccag
    10681 gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct tctccgtgag
    10741 ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa ttttttccca
    10801 tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag caactaataa
    10861 aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat ccctctccgt
    10921 tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa
    10981 cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct ctcatttatc
    11041 aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt ctccagtgca
    11101 ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg ttctgttgga
    11161 ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat cgatgataca
    11221 attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag ttcattttgt
    11281 aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg cttttgataa
    11341 gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc attaaatctt
    11401 ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg agctagcggt
    11461 gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa
    11521 gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat taattgattt
    11581 tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg atcattttct
    11641 gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac catagtttct
    11701 gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg aatttgtgca
    11761 attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg cgagtaaaca
    11821 gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact gaacgatatt
    11881 tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa atctttagga
    11941 agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata tactttagct
    12001 gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc atacgtatcg
    12061 ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc tccaactatc
    12121 aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat ctcagttttg
    12181 tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac aacagtgtat
    12241 cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc tggttcatca
    12301 aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg aaaataaact
    12361 tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga atcagctact
    12421 acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac aaggacttca
    12481 ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc gattccgtca
    12541 gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt atctgaaaca
    12601 cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa ccgacgaagt
    12661 aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg ctcaactcgt
    12721 tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct tactgagcca
    12781 atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata ttcaggtaat
    12841 tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat attgtggaat
    12901 ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat tgacttttga
    12961 tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag catttcaact
    13021 gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc taaggtcatc
    13081 attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc tcccaatcct
    13141 ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg
    13201 aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg
    13261 tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc actgattcca
    13321 atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt ttgtgcatac
    13381 cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt aagcttttgc
    13441 ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca aaagtttact
    13501 ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga caatacgatg
    13561 gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat tatatgtgca
    13621 tttaccatat cagattctcc tactttcctt cttctactga atttacttgt tgttcttttt
    13681 tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc ccaataacaa
    13741 ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag gttgaaagca
    13801 ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca gtgcccacca
    13861 cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa
    13921 tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat tcatcataaa
    13981 gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca attcctaata
    14041 acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca aaagcatatg
    14101 cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa atagccgaca
    14161 tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct gttgtcattg
    14221 cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat gtagtaacaa
    14281 aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt acttttttca
    14341 atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata aatcccatac
    14401 caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga cggtcagcaa
    14461 ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa
    14521 tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg ccaccaaaaa
    14581 tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata taggaaaccc
    14641 cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt agtaactccc
    14701 ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt ctcttcacca
    14761 tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt
    14821 tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac atccgaaatt
    14881 gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc taaccctttc
    14941 ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat ccctgcgata
    15001 catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa tgatagagct
    15061 tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc gtcatccatc
    15121 aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc agtgtctttt
    15181 attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca ttcaacagac
    15241 cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt gccatgagga
    15301 atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt
    15361 tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga taatttttcg
    15421 actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat attgattttt
    15481 tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa cgacgaactc
    15541 gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt ttcataaatt
    15601 ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga atgaacaagt
    15661 gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg cttttttata
    15721 aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga tagtttttct
    15781 cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac caattttttg
    15841 gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa aaagcaaaat
    15901 cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg tagatactgt
    15961 tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac aggaaaattt
    16021 gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct atcttgtagt
    16081 tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt atttattctt
    16141 tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac tttcgttttg
    16201 tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc gatttcatct
    16261 tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac acttatcata
    16321 aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat atttactccc
    16381 aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat caattcttca
    16441 tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac acccatatct
    16501 aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc aatcgaagta
    16561 atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa tgctttttca
    16621 atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt attactacgt
    16681 gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat agctaaagtt
    16741 tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt aagcaattcg
    16801 gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat gaaattgata
    16861 ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca ataatagttc
    16921 acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg cacgccattt
    16981 cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc tttcattgtg
    17041 ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat ctttagagaa
    17101 tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg agagtctgcg
    17161 atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc aataagcgaa
    17221 acgccttttt taggttgaac caacatttgt acatctggca ttttttggat tatttctttc
    17281 aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc tgataattct
    17341 ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg ccgttcgcta
    17401 aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat tattatctct
    17461 attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc ttgattattt
    17521 caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta ttaaatatat
    17581 ataagataat acatttttct actagttctc actaatcttg tataattaaa ccctccgcaa
    17641 cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc
    17701 ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct
    17761 ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg ttcctggttt
    17821 gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg tcacctttgc
    17881 ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat
    17941 gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt
    18001 tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga
    18061 aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg
    18121 atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg
    18181 tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg
    18241 agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg
    18301 aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat ctactaggtc
    18361 cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga
    18421 aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact
    18481 tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac cccactcccc
    18541 acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat
    18601 gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat
    18661 ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc
    18721 ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct ttgtccagta
    18781 agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac
    18841 gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc gtcacatagt
    18901 catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa
    18961 cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc
    19021 ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc
    19081 cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta
    19141 aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg accacctact
    19201 taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta
    19261 gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat
    19321 acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga
    19381 aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac
    19441 tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct aatgtcagca
    19501 gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt
    19561 caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc
    19621 caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc
    19681 gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc
    19741 acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc attaaaatcc
    19801 ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat
    19861 gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga
    19921 cccacatttt acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt
    19981 ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac taggtgaata
    20041 ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac ttggtcaatt
    20101 ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata accaagagag
    20161 tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat
    20221 tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg caaaatacca
    20281 tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg
    20341 ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc
    20401 atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca
    20461 atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt
    20521 tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct
    20581 gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc
    20641 gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa
    20701 ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca
    20761 ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac tttatctaat
    20821 gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca
    20881 aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg ggtataaaga
    20941 aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa
    21001 tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt atcagtttcc
    21061 tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga aaatttttgc
    21121 ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa ctatagcaag
    21181 actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa
    21241 gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg ttgttatcgt
    21301 tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga ccatataaaa
    21361 agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca tgccgtttgg
    21421 gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca atcccttctt
    21481 ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca
    21541 tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    21601 ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg tttgtttata
    21661 gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa acacaaaaaa
    21721 ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac tgcgctcagc
    21781 attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc gcaattaacg
    21841 ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct tcgtatcatt
    21901 gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt ttattatatt
    21961 tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc agtatatttc
    22021 gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa
    22081 aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct
    22141 taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac
    22201 tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc
    22261 tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc
    22321 tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc
    22381 agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa
    22441 tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt
    22501 agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa
    22561 gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag
    22621 tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa
    22681 gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac
    22741 ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat
    22801 tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag
    22861 tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc
    22921 tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat
    22981 tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt
    23041 tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac
    23101 tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat
    23161 taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat
    23221 taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat
    23281 tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc
    23341 tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac
    23401 cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg
    23461 aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt
    23521 aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg
    23581 gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga
    23641 taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga
    23701 tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg
    23761 ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt
    23821 agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata gccttgatga
    23881 aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac
    23941 ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc
    24001 tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc
    24061 attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga
    24121 gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact
    24181 tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac
    24241 ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca
    24301 agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc
    24361 attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt
    24421 aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga
    24481 gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc
    24541 gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc
    24601 agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa
    24661 aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta
    24721 tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag
    24781 cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac
    24841 aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt
    24901 tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat
    24961 tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga
    25021 ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg
    25081 aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa
    25141 tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga
    25201 agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc
    25261 tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt
    25321 tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg
    25381 ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc
    25441 tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga
    25501 agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg
    25561 ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg attggtcgca
    25621 gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg
    25681 aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg
    25741 ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg
    25801 atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt
    25861 caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa
    25921 atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg
    25981 cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt
    26041 atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc
    26101 gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc
    26161 cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa
    26221 ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg
    26281 aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt
    26341 tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag
    26401 aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca
    26461 ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg
    26521 ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt
    26581 gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa
    26641 ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta
    26701 ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat
    26761 atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt
    26821 ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt
    26881 gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat
    26941 tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga
    27001 accagagaga ctatcagtta cttccccaaa atctattatc gaatgaaaga tggattactg
    27061 tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag
    27121 aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg
    27181 gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca
    27241 gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat
    27301 atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa
    27361 gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct
    27421 gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg
    27481 gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac
    27541 aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa
    27601 tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc
    27661 aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg
    27721 ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg
    27781 ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt
    27841 atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt
    27901 tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag
    27961 atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta taatactttt
    28021 atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat ttagagtatg
    28081 caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc cccgaagttg
    28141 gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag gttgccattg
    28201 gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt cgcacccagt
    28261 ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct tggttggtag
    28321 agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat gcaacttttg
    28381 caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt cgagtgacga
    28441 ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa cgtgaaattg
    28501 cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa tcaagagaga
    28561 acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata ataaaagatt
    28621 atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta tttttaccac
    28681 tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga acaagtaacg
    28741 aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc tactgcaaaa
    28801 gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca acggaaatta
    28861 gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag agcaaaatgt
    28921 tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga tttattcgcc
    28981 ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat cggtttttgt
    29041 gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag taaggaggtg
    29101 gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg accagtggac
    29161 atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg gcaaagattt
    29221 tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct tctccgacct
    29281 aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg ggctacgttc
    29341 gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta ggtgcttaac
    29401 tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca ttctcgagac
    29461 gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga tcaaaacatc
    29521 agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt ctatggttca
    29581 gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact gccagcaagg
    29641 gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa tcgagtttat
    29701 gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa aatcatgatt
    29761 cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa tctggctttt
    29821 cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac cttactaaca
    29881 ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac acctagagag
    29941 tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca agtcaattca
    30001 gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat ttttgatatt
    30061 gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg gagaaattta
    30121 ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc gacggttaaa
    30181 atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa tgaatgagga
    30241 gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga ttgcggacca
    30301 agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt tggaagtaaa
    30361 tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct gaccgcggag
    30421 gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc gttagccgac
    30481 catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa taagtaagca
    30541 accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt gttaactcat
    30601 ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga atttagatta
    30661 tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat tggctacggc
    30721 ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatctat
    30781 aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt tgcctttgac
    30841 gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa aaattattca
    30901 agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct tttactccta
    30961 tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag aagataaagg
    31021 tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt tactcgctca
    31081 aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg tgatgaaacg
    31141 cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc taacgaaaaa
    31201 atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc gttccgggga
    31261 acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg gcagtctcaa
    31321 gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca agttagggat
    31381 tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa atgaccgtgc
    31441 ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca cgttttccat
    31501 tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa ccaactggcc
    31561 cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc aattaacttc
    31621 cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac aagtcgcccc
    31681 tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata tccaggatat
    31741 ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac aaagtatggc
    31801 aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa agatattttt
    31861 ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa tgcaagtgca
    31921 gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg gacaaaattt
    31981 atgtgcgact cgttccttag tgaaaagcta aggcactaat aaaagaacga aaaagtttgg
    32041 ttattaggag aactgataat ctgacaggtg gaatgatgaa gtaacgctct gaaacgcctg
    32101 ccctgagcct ccgacaagca ttgtatgagg aaactcagaa tgtttgaagc tcggtaaaga
    32161 cggctgaaag ataccgtaat agttgatgaa catatatctt ctatcgaaaa ctatccagag
    32221 gtgggtagcg tatccaacag gtcgggggtc tataaaatat ctatggttag aatgttctat
    32281 aaggaactga cgaaacagcg aatgtacggg tctaaatttg aactttgtag aaatgcaaag
    32341 gtacgagaaa tcgtagctgg tgtggcaaag taagaatata ggttatgaaa tgccatataa
    32401 cgttacaggc ggtatcaagc cagcaggctt atagctagtg acctaaggat atatatgtat
    32461 agataagatt atcggaacga ggaaaggtat tgggttctca tcaagagaat aaactgacga
    32521 agaacaataa gcagttgaac taatgctgaa aagcagaggg cgaacctaag aaattcttgt
    32581 aatgagaatg gaggaatgcc ctcaagtcgt gtatatatta gaaaagtatt atgttgtttc
    32641 aataaggatt acgagtaaga taaaaaagct cactcaattt caagtaaagg agtgaagcct
    32701 tatgcctaaa aataaaaatg aaacattgtg tgtagaagat ttaagacacg ctgaatatta
    32761 cgaaatgcaa aacacctttg atgatttata tgccaaaagt aagaatggag atattttcac
    32821 tcatcttatg gatattatcc tatcgagaga aaatatatta ctggcatatc gaaatatcaa
    32881 agcaaatgca ggcagtaaaa cagcaggaac agacggtaca ataattaaag atattggcaa
    32941 attgccagca gaaacagttg tcaaaaaagt aagatacatt gttgcaggca gtccacatgg
    33001 gtatcgacct aagccagtga ggcgaaaaga aataccaaaa ccaaatggta aaaccagacc
    33061 tctaggtatt ccttgtatgt gggacaggct tatacaacaa tgtataaaac aagtattaga
    33121 accaatctgc gaagctaaat ttagtgaaaa cagctatggt tttagaccta ataggtcagt
    33181 tgaaaatgca ataaaagcaa cctataaccg cctacaaatc agccagctac attatgtgat
    33241 tgaatttgat attaagggtt tctttgacaa cgtgaaccat tccaagctga taaaacagat
    33301 atgggctatg ggaatacggg acaagcactt gatattcatt ttaaaaagga tattaaaagc
    33361 tccaatcaaa atgactaacg gtacaataac atatccagag aaaggaactc cgcaaggggg
    33421 aataatatcc ccactactag caaacattgt tcttaatgag ttagaccatt gggtagaaag
    33481 ccaatggcag gaaaatcctg ttacaaaaaa ctatgtggtt catatcaata aaagcggtag
    33541 cccatgtaaa agcaacgctt acaaagaaat gaagaaaaca aagttaaagg aaatgtatat
    33601 ggttcgttat gcagatgatt ttagagtttt ctgccgctac aaggaaagtg ctgaaaaagc
    33661 caaaatagca atcactcaat ggattgaaca acgattaaaa ttggaagtat cacaagaaaa
    33721 aacaagaatt gtaaatgtta gaaaaagata ctccgacttt cttggtttca agattaaaat
    33781 gataccgaga agaaagaagt tagttgttaa atcacatata agtgataaac aattcaaaaa
    33841 ccaaaaagac aagctggtat cacaggcaaa gaaaattgct tctccctcaa aagggaaaac
    33901 ggaattagaa gaaacaagac tttataattc aatggtatta ggtatgcaaa attattatca
    33961 gattgcaacg aatgtaaatc tggattgtat gaaactgaat agagctgtaa tggctatctt
    34021 cactaacaga ttaggtacaa acaaaagggg cagattgaga aaaacaggca gacaacttac
    34081 gacaaaggag gcaacaagat atggtaaatc taaaatgctt cggtatgttg ctggcgaggg
    34141 cgaacctatt ttccctgttg gatatatcca acacagaaaa ccaatggcaa aggtgtattt
    34201 agcgaattgc tacactctag agggaagaaa atttatccac accaatctaa gtgttgataa
    34261 attactgatg taccagttaa tgaaattatc tttggaaaat cgcacaatag aatacgcaga
    34321 caatcgaatt tcgctatttt ctgctcaaca tggaaaatgt gctataactc tggaagaatt
    34381 tcaacaagct aatgaaatac attgtcatca tattgtacca actggagctg gtggaaatga
    34441 cgattataaa aatcttattt tggtaaaaga ggcggtacac cgtctaatac acgctaaaac
    34501 agaagaaaca atacaaaaat atatagctct attgaagcta aataaagtcc agctcataag
    34561 actaaataaa tatagagtat tagctggcaa tcaagaatta aacttgatat aggtctacta
    34621 cataaatact tttcaatata aacacgatgg aacgcggagt gcggtgaaag tcgcacgctc
    34681 cgtgtgaatg gggggaaaag tttgagatat ttataatatc aggaacttac ctatccatat
    34741 atcaatggtt tttaaccaat gcccaatcct tagtcttact ggcaattgtg gtcattggtt
    34801 tgtttttagg atttaagcgg gagttttcca aactgattgg ctttttggtg attgctttga
    34861 ttgcggtcgg attggtcttt aatgcttctg gggtgaagga tgtcttactc agtttgttta
    34921 accgaattgt cggtgcgtaa gggggaagca cgatggaaca gatgcgtgtt tatattgcga
    34981 acctcgggaa atataacgaa ggtgaattgg tgggggcgtg gtttacccca cctgtcgatt
    35041 ttgatgaagt gaaagaacga attgggttaa atgacgaata cgaggaatat gcaattcatg
    35101 attatgaact tccctttgag attgatgagt atacgccaat tgaggaaatt aatcggttgt
    35161 gtggcttagc ggaggaactt gaaggaacgc cagttggtga agttgcttct gagattcaac
    35221 atgctttctt caattctttt gaggagatgg tagagcatgt ggatgatatt atctattatc
    35281 cagattgcga tgatatgagt gatgtagcat atttcttgat tgtagaagct ggtgattttg
    35341 gagaagtacc agaacacttg aagacgtata ttgattttga tgcgtgtggt agagacttgg
    35401 agattggagg caattatttg gtaacgagtc gaggtgtttt tgagtatcca tattaacaaa
    35461 attattgaag ggtagttgat ttctaaacta tccttctttt taaaggaatg atttgatgaa
    35521 gaaaataaaa agctacacca gtatttgggc agttgaaaag gtcatttatg caattaatga
    35581 ctttcaactg ccttttcctg tgacttttaa ccagatggct tggtttgttt tatccttgct
    35641 gtttgtcatc gtatttgccc atgtgcctcc actatctatg atagaggggg cctttttaaa
    35701 atacttaggt ataccggtgg cagttacttg gttcatgtcg caaaaaacgt ttgacggtaa
    35761 gaaaccattg ggatttttac gatcttttat tagctatcac ttacgattta aggtcacttt
    35821 cgcaggaaag aaagtcaaag aacagaagaa acgatgggac gaacccatta ctctggtaag
    35881 gagtgtgaac tatgtacccg attaagtata ttgaaaataa tcttgtcttt aatcaagagg
    35941 gagaatgttt tgcctactat gagttaatac cttacaatta ctcctttttg tcacctgaac
    36001 agaaatatca agtccacgat aactttcgtc aattgattgc gcaaaatcgt gaaggcaaga
    36061 ttcatgcctt acaaattgcc acagaaagta gtattcgtgc cacgcaagaa cgatcaaaaa
    36121 aggaaattac cgggcgatta acagaagtag cgaaacaacg aattgattta cagacagagg
    36181 cattagtatc catgattggc gatagtcaga ttgattatcg cttttttatt ggcttcaaac
    36241 tgattgcgac agatgaagag gtcaatctaa agaatctcaa gaaatctttc ttctccggct
    36301 ttcaagaatt tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca
    36361 atgaagaaat tcgccgatat accaagcttg aaaaactaat ggaaagtaaa ttggctcgaa
    36421 gatttaaagt cagacgagtc acaccgagtg atctgatgta tttgattgaa catatctatg
    36481 gcgaaaaggg tacaccattt gaagaatatg aatttcagct accaaagaaa aagctgaagt
    36541 cggaaacttt ggtgaaacgt tacgacttat tacgtcccag tcgttgtttg attgaagaaa
    36601 agccccggta cttatgcatg gaacatgaga atcatgaatc atatgtagcg tacttaacaa
    36661 ttaatacaat tgtgggggaa atggagtttc cctcctcaga acttttctat taccagcaac
    36721 aacaatttac attcccaatt gacactagta tgaatgtgga gattgtgacg aacaaaaagg
    36781 cacttgctac cgttcgcaat aagaaaaagg aattaaaaga cttggacaac catgcctatc
    36841 aatctgacaa tgaaacaaat tccaatgtct tagatgcatt ggattccgta gatgaattgg
    36901 aaactacctt ggatcagtcc aaagaatcca tgtacaaact gagctatgtg gttcgagtaa
    36961 gtgccgaatc agtagatgaa ctaaagcgcc ggtgtgatga agtgctagat ttttatgatg
    37021 ataccaatgt aaaactagtc cggccttttg gcgatatgat ggggctacat gaagaattct
    37081 taccttctag taagcgatac atgaatgatt atatccagta tgttacgtct gattttctcg
    37141 ccggacttgg ttttggcgcc actcagatgt taggggagtt ggaaggcatt tactttggtt
    37201 ataacgtaga tacggggcgc aatgtttacc tgaaaccggc attggcctca caaggggtca
    37261 agggttcagt taccaatgcg ctagcagcgg cattcctcgg ttcactcggt ggcggaaagt
    37321 cctttagtaa caatctctta gtttattacg ccgtcctttt tggtggacaa gctgtgattg
    37381 tcgatccaaa agggggtgcg aaacgttcct acttgaaaag agtaggcaca gcacttcatt
    37441 aaatcaatat ttaatgtgtg ttggagaact aaacacacga atagtcgaat gacggggtaa
    37501 cgccctgaaa ggctacttta atactctgac tggcagaaac ttgagtaaac aaggctctgt
    37561 cagaagctcg gtgaagtcgg cagacagaca ccgtaacaga taatgctgac gaaagattcc
    37621 cagaggtggg cgttgtgtcc tatatgccgg aagtctataa aatatctatg gttagaatgt
    37681 agatgagagg tctactgacg aatccttgaa tgtacaggtc tatatcgggg ctggatagaa
    37741 atgtccagtg acacaaacag tgtcgctact gtgggtgagt aagactccgc atttatgaaa
    37801 atccatataa cgttaaaggc gttatcaagg tagcaggctt atagaggaaa cctaaggata
    37861 tatgtacaga tagtgtgttt ggaacgttga aagctgacaa catggagatt ttactgtctg
    37921 tgaagtgttc acatcagaaa gttattgtga gattagcaat gatataaggt gtgttaatgt
    37981 cagtgagagt ggtggcacag tacctatgaa accgtgataa caagcggtgg agggatagcc
    38041 actagtcata tgaagaatag aattactaag aaaaatggta tgggttcgag taagactaag
    38101 agatgtactt gctcctgcaa acaaggaggg tacagaccat gctaaagaaa aacaagctcc
    38161 gctataacga gtattatgat atgcaacata tttatgacga gttatacgca cagagcaaga
    38221 acggtaataa cttttataag ttacttgaaa ttattggttc ggaacaaaat atttgtttag
    38281 cttaccgtaa tctgaaatca aacagtggca gtaaaacggc tggaacggat ggaatgacga
    38341 tagacgatat aaaacaactc agcaatgctg agattgttgc taccgttcgg gaaagcctta
    38401 gcaactaccg ccccaaatca gtcagacgtg ttttcattcc aaaagctggg agcgataaaa
    38461 tgcgtccatt ggggattccg tgtatctggg acaggctggt acaacaatgt atcctgcaag
    38521 tcctagagcc gatatgcgaa ccaaagttcc ataatcattc ctatggattc cgtgcgaatc
    38581 gaagtgccca ccatgcagtc agcagagtaa cgacactgat aaatttatca aaatatcatt
    38641 actgtgttga cgtagacatt aaaggatttt tcgataatgt caatcatgga aaactattaa
    38701 aacaaatctg gacacttggt attcgggata aaagattgat ttgcattatc agcaaaatgc
    38761 tgaaagccga aattgacggc gagggtgtcc cagaaaaagg aacgccacaa ggcggtctgt
    38821 taagtccact gctctccctc atagtcctta atgaattaga ctggtgggta agcagtcagt
    38881 gggaaacatt ccagccgaag aaccgcagta aaaatgggtg gcttcaatac gcaaagaaat
    38941 ataccaaact gaaaagcggt tttattgtac gttacgctga tgattttaaa atcatgtgtt
    39001 caacctatgg agaagcacaa agattttacc acagtaccgt agattttctt aataaaaggc
    39061 tgaaattaga aatcagtcca gagaaatcaa aggtagtaaa tctgaagaaa aattcatcag
    39121 attttctggg gttcaaaatc aaagtcattc caaaaggaag aacgaaacac ggttatgttg
    39181 ccaaaacaga tatgaaccaa aaagcattaa agaaagcgaa aacaaatcta aagttaaagg
    39241 taaaagatat agccagacat acgactggct tcaatatcag cagatataat ctgacagtta
    39301 tcggtatgca gaattactac tgcatagcaa ctaacgtata taacaacctg acagaagtaa
    39361 gttacgctct cttaccgacc atacgaatcc gtttaaggaa cattgcaaaa tcagttccat
    39421 ttgaaagtac aagttcagaa tttcagagca ggacaaaagg aatcagacca aaaacaaaaa
    39481 tagtgatgat tgcggataat ccgctattgc cgattcaagg agtacaacac aaaaatccta
    39541 tgaatttctc gcaagatatt tgtaatttta caaagcaagg gagaaacaag gtgcatgagg
    39601 acgttgtagt tgtaacaaaa gaagaaatcc gagcgttgtt ggaaaatgaa aaccctgccg
    39661 atagcgtaga gtttaacgac aatcgtattt cggcatatat cgcacaacaa ggcaactgct
    39721 atgtaatgaa tcgtcgagga actccgtcca ctctgatatg tattcacaaa tcagacaaag
    39781 gagataacct tgacagatat tctaatctgg catttgtgga aattcccatt gcaaaggcaa
    39841 tcctaacaga atcagcagac gaagcaaaat cactgttaaa aggttatgtt cttaatagtc
    39901 agcagaagaa aaaactgaac agaatacgga cgaattacgg ataccagaca ataactggga
    39961 aataacttat aaatctattt ggatatgatg gaacgccgta tgaagcgaaa gtttcatgta
    40021 cggtgtgaag cgggggaaaa cttggagata acatcaaaaa gttacctatc gctataacgg
    40081 ggtggctgga aagaaacctt gccagaaatt gcggatgaaa tcaatattct caacttaact
    40141 agtaagccaa aaaatcaagg attacttgat ccttatgtga ttatggagaa aaagaaggat
    40201 tctgaaagtc tagcaattga tattctgacc tttttaacag gaatttctag tcgagatggg
    40261 gagaagtttc cagtgttgcg taaggccatt cggacggtta cccaaagcaa tcaacgggga
    40321 ttattacaag taattaatga gcttcgaaaa gaaggctcac cagttgcgga aaatatcgcg
    40381 gatcacatcg agtcctttac cgattatgat tttgcccatt tgcttttctc agatggttcg
    40441 attacccagt cgattagttt agataggcag ttaaatatca ttcaagtagc ggatttggtt
    40501 ttaccagatg cagaaaccac ttttgaagaa tataccacca tggaattgct tagtgtttcc
    40561 atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaatcg agaaatcttc
    40621 aaaattgtag acttagatga ggcgtggagt tttcttcaag tagcgcaagg gaaaacatta
    40681 tcgaataagc tcgttcgtgc tgggcgggct atgaatgccg gggtttactt tgtcacgcaa
    40741 aatagtgatg atttactgga tgaacgcttg aagaataata tcgggttgaa gtttgcgttt
    40801 cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggctt ggatcgagaa
    40861 gatgaaagca atcaaaaacg cctacgagat ttggaaaatg gccaatgctt gatgcaggat
    40921 ttgtatggtc gggtaggcgt ggttcaggta catccagtct ttgaagaact cttccatgcc
    40981 tttgataccc gtccacctgt tcaagagaga atggagtgag gggatgaaaa agaaactttt
    41041 tcggattgtg ggaatcgttt ttattagcat actcactctg atagttctat tgagtttagt
    41101 tggtacggta gccgaggcga cagggctagt tgatgataca gttgaagcag gaaatctata
    41161 ttccaaatat tcactgaaca actatcaact agatttcttt gtggatagct cttgggattg
    41221 gttgccttgg aattggggtg atggcttagg gaaaagcgtg atgtatggtc tttatgccat
    41281 tactaatttc atttggacgg tgagtttgta tttgtcgaac gccacgggtt atgtggttca
    41341 agaggcttac aaattagatt tcatttcgga tacggctgaa agtattggaa agaatatcca
    41401 aacattggct ggtattactg aaaatggact ccagacctcc ggattttatt ttgggtttct
    41461 attattgatg attttagctc tgggggtgta tgtggcgtat accggattat taaaacgaga
    41521 aaccacgaaa gcagttcgag ccgtgattaa ttttgtgatg atttttctac tatcgggttc
    41581 gttcattgct tatgcgccta cctatatcac aaaaatcaat gactttagct cagatgttag
    41641 tgaagcggcc cttacccttg gaaccgagat cgttgtaccc aattcagaaa gccaaggaaa
    41701 agatagtgtg gatttgattc gggatagttt gttttcgatt caagtccaac aaccttggtt
    41761 actactgcaa tttgatgatt ccaacataga agaaattggt gaagaccggg tcaacaagat
    41821 tctatctgtg agtccagatg aaaacaaagg caaagatcgg gaagaagcgg tcaaggcaga
    41881 gattgaagac aatgacaatg cgaatctcag tattaccaag accatgaacc gtttagggac
    41941 cgtggtcttt ttggtattgt tcaatattgg catttccttc tttgtttttc ttctaacagg
    42001 gattatgttg ttctcgcaaa ttctcttcat tatctttgcg atgtttctgc caattagttt
    42061 cttattgtcc atgttgccga cttatgaaag tttaggaaag aaggcgatta ttcgtttatt
    42121 caatacgatt atgatgcgag cgggtgttac tttggtgatt acgactgcat ttagcatctc
    42181 cacgatgttt ttcaatattt cggcaaccta tccatttttc atggttgctt ttctgcaaat
    42241 tgtgaccttt gccggtatct atttcaaatt gggcgacatt atgagtatgt tcaacctcca
    42301 gagtaatgat agccagtcaa tgggaagacg cgtcatgcga aaaccgcaaa tgttaatgaa
    42361 tcgaaaattg cggcaactca atcgaaatgt gggacgagct ttggcttttg gtggtggtgc
    42421 agcagtcgga aataagttgg caaaggaaca atcaaaatcc aaatttaagc catccggttc
    42481 ttctctacga aagaattcac gattaccgaa cgatcacgaa atactatctg acttaactaa
    42541 ggagaatcca gtttcaaata ataagaaaca atcccgaatg aatttggctg ggagaaaaat
    42601 cggcaaagtt ttggataccc aagctttggt gaaagataag actaaacaag taaaggatca
    42661 ggtaaggaat acgccaacga atttgaaata caatctccat aaaggaatcg aaaagtcgaa
    42721 aaaagcacca gaagaattca agcgcggact gttccaagaa aaagcggatc gagtggattt
    42781 gcgagaaaaa caacgacaac gaagaaatgc gaaaatggac gaaaaacgca agatactgag
    42841 tgaagcaaca aatcgtcagg gaaaaagaag ggcgaatatt ccaatagata aagcagctca
    42901 acctcagaaa gatatgatgc tgaaaaaaga agcaccaaag cggattgtga gagcaaatct
    42961 gaatcccgaa atcaaacgaa aactaactag tcaagaaatc atttcaaaag ggaaaagcca
    43021 atcagcaagt aagaaaaatt ctttccaaca agtgaaacag aggtccactc tgccaaagca
    43081 gaccaatcaa aaagtacaaa aaagaatgaa ccatcgtcca aaatcaaggt caggtgagaa
    43141 aaaatgaact tgaagaaagt gggtgttatt tcattaactt ttctgaccat ctcttttatg
    43201 ggtattttga tgtgtgtggg tttgttgttt ggtgaagaca gtgagggaaa tggtagttct
    43261 ggcacttctc ctggcggaac tagtgtctca gaagaagtat tgaaacaccg tgcaatggtg
    43321 gaaaagtatg ccaaagaatc ggatataaca gagtatattc caattctttt ggcgattatt
    43381 caagtggaat ctggtggcac aatggaagac gttatgcaaa gttctgagtc agccggatta
    43441 ccacctaatt cattaagtac cgaggcatcc atcaaacaag gctgtctcta ttttgcgtct
    43501 ttggttaagc gttccaaaga actagggtgt gatcaagata gtattattca ggcctataac
    43561 tatggtggcg ggtatctaga tcatgtcggg aaaaacggga agaagcatag ttttgcttta
    43621 gctgaatctt tttcaaagga aaaatcaggt ggtcaaaaag tcgattaccc caacccaatc
    43681 gcgattaagg aaaatggtgg gtggcggtat aattatggca atatgtttta ctgtttatta
    43741 gtgaagcaat acctaactac aactcagttt gatgacaaaa tagtccaaga aatttttgat
    43801 gaggcattta agtatgaagg aacggcctat gtgtttggtg gctcaagtcc agaaacgggg
    43861 tttgattgta gtggcttgac ccaatggtgt tatgccaaag ttgacctgaa acttcctcgg
    43921 gtggctcagg atcaatatga tgccatgact catattgatc taaaagatgc caaaccaggt
    43981 gatttagttt tctttcattc aacctatgat gccggtactt atgtgaccca tgtgggcatt
    44041 tacgcagggg agaatcgcat gtttcatgca ggagatccag tggggtggac gaatctaacg
    44101 gaaaactatt ggcggcagca tcttatcgga gctggccgat acaaataaga gaaaagagga
    44161 aaaagaatga agataaaaat tgaacggaat caaaaggaaa agagcccaaa aaagaaaaaa
    44221 gaacgagttg tatccgtggg taagcatcga aaaatggtgc ttgccctttg gcttttattg
    44281 tcttgtagtc ttgcttttgg cgtttataaa aatttcaccg ccattgatca acatacaact
    44341 catgaaaaag tggttataaa agagaaggta gtgaatactt ctggcgttga aagttttaca
    44401 gaggattttg cgaaagaata tttttcatgg aaaaataaca aagaagcaat tgaaaagaga
    44461 atgagtaatc tcgaacagta ccttacagaa aacggtctcg ctttaatcca agatatggtt
    44521 cgagcagata ttcccacgag ttccgaagtt caatctgtaa aaattctaga tgttgaaaaa
    44581 cactcggaag aatttgtcgt ttcattttta gtggagcaac atattacaga gggaaaaaag
    44641 acgcaatcaa tttcttctgc atatcgagtg acaatctttg aagatgaaaa caggaatcat
    44701 ttagtaacta gtttgccaac aatgattgga aagccagaca aggcaaaata tgaagcaaaa
    44761 caagtagaaa acgattcaga aattgatgcc aaaacaacgg aagaaatcac cgatttttta
    44821 gaaacatttt ttaaacttta tccatcagct tctgaaaaag aattggagta ttatgttgaa
    44881 aatggtgcga tgcgaccaat caatattgac ttaaagtttt cggagttaga gaatccagtt
    44941 tatctacaaa acggaaaaaa ttttctagtg agtacaaaga taaaatacct tgataatgta
    45001 tcaaaaacca ctagctactt tcagtacaaa ttagtattac aaaaaaatgg aaactggaaa
    45061 atcgttgatg ttaaataata atgactcaat ctaattttat tagaagttat aagtagtgta
    45121 acttatttca ggcagaagtc gcccatcctc tataggtggg tgatgaatgc cattcggtaa
    45181 ctcgaaaacc gacaattaag acatagtgcc tttttatttt aatccatgtc ataatcagtc
    45241 tatataggaa atggttgata taccatggaa ttagatacaa ataatcattc agtttttctt
    45301 cttggttacc cccttatttt agtagtgaaa cattgtaaac atgtcattga tgatgtaatg
    45361 tcagcttatg caaaaactgc ctttgaaaga atttcagaat cacatcacat taccttagat
    45421 gaatgaaatc gtgataaaga atactttcat attatgctca aagcacaacc taaaaccgaa
    45481 ttaacaaaat tcagcaatgc ctaagctcac gtctgataaa atgagacttt ctaagggtca
    45541 aaaatttcct ttgaatacga ttattgctta gataaaagta aaagccttag aactacgggg
    45601 atagcttggt taataagaga tatcgttgtt agtaaagaca taggcttgca agtatgctct
    45661 attcccaaga atctccccct tcaagcacca aggatcgtta aattcagcta agtggcaagt
    45721 aattcactag acgataatta tctccatatt tcaatctcag cttttccatt tttcatagca
    45781 aattccgtta tttttatagc atctaaaaga ttcagctttt ccactgatct ttcaccactt
    45841 ttcagctttt ttattgtgct taaattgata ccagtacctt ttgatattga attggaagta
    45901 aaatcattaa gggttttttt tattaaattc atatccgcaa ttccagcgtt cataagaatg
    45961 aagattctcc tctcatagac tactttgaaa atggtactac agtggctgtt tatatatttc
    46021 agtaggttac tgtagtggtc atcttattat aattagaaaa ggaacctaaa tcaaaaatgc
    46081 taaagaaaat tttcatagta ttaagcctct catgactaaa gccacggaat tcttgataaa
    46141 agagtatact tgcaagccta aacagcggta tctcttatta accaagctat ccccgttgtt
    46201 cctaaggttc ttgatgtttt ctaaggcaat gcttgtattc atagcccttg gtcaaaatat
    46261 tgatactatc gttgacgttt cgattatgat gtatacggca aataggacaa gtccattctc
    46321 gaattgtgag tgacttctta ccatctgtgt gtctacattc tgaaccaatt tgactagacg
    46381 gaaatcattt atctatctta atgatttttt gacagaagta tcgaaaatct ttacataatc
    46441 tgggtgatgt attcttatgc agatgagagc gttggctccg tattcataac cagtagcctc
    46501 taaaacgtag tccatcggtg ggatacctag cttgcggttg ccagcggctt tcgcagtctt
    46561 cttgtgatca aatcaagcat ctcattgagc actagtattt tgaagataat acttgagttt
    46621 ttcggctttt agaagttcac ttcatcttcc atcatccatg gaaatcccac tcacccatac
    46681 gcaggcctcc aaaaattctt caacagtggt tttttcagac atttttctga atacctcctt
    46741 gttttttgat acgccagata gtcaggaaaa aggcaacaaa acacaaaaca aagctgccta
    46801 aaaaggtaat cttcattcca tataaaaaga catccggccg atttgcgata tagttagtta
    46861 cttgctggcc catcttagca gacatcgctt gatataagac cgtcgtagaa agcgaaatcc
    46921 cgctgaccat ccccaaattt cgggcaaagg aattcatcga gcctgccagt cccaagtttt
    46981 cgatagtgac cgagctcata accatcgtat tattggggga ctggaacaag gcattgccgg
    47041 cacccatgat ggcagtagcg acaatgtata gccacaaagg agagtccact gacaaaaaca
    47101 tatacatgac ctgggttacc gacaataagc ccaagcctac taataccaaa atctccgggc
    47161 caacccggtc ggtcaggtag ccgcttaaag gcgccccgac gaccatcatc aaaggaaaga
    47221 ccatcatcaa cagcccagca tagctaggac tcaacccgcg agcattttgc agataaaaag
    47281 gaatcactac attgacaaag aagttggaag aaaaaatcaa caaggctgta atcaagctca
    47341 tggtaaaaat cttgttctta aaaataccga aagaaatcaa tggagaggct gctttctttt
    47401 caacttttaa aaagctgaca aaggcaatca ctgaaattac gaacagtaca agggggactg
    47461 gatggaaaaa gccactttct tgccccaaaa agattgctcc aaaaaagctg ataatcgtag
    47521 ccgcaatggc agcaaagccc cctccatcga ttgtatggcc gctcatggtg atatcttttg
    47581 gtaagaattt ttcagcaatc aacatcgtta cgattcccac cggtacattg atccaaaaaa
    47641 tgtacgccca agaaaattgg gacaaaatca agccgccaag acctggtcca gcaatcgagc
    47701 ctaatgaaac aaacgcacct attgctccca atgcccgacc acgttcatgt atgggaaaga
    47761 cttccgtgat gattccggca tttgtagcca tagtcatact agcaccaatg gcttgtacca
    47821 cccgtgcgaa caataaaata cctagcgaat gattgaaccc acacaacagc gatcccaaga
    47881 caaagatcag cgtaccaatc cgaaatactt tgattttccc acggctatcg ccaattttac
    47941 caaacagcaa caaacaagaa cagacaatca tcagataaac ggacactacc cattccgctt
    48001 gattcattgg tatgcccata tcatctgaga tggtaggtag tgcaatattg acaatactgc
    48061 catccaatgt ggacatgaag gtaaacatag atatagcaat caaaatccac cagcgatttt
    48121 tttggacttt tgcatcttct tgataactat acataatagg atttcctttc taatgatcgt
    48181 gtagctaaga acaatataac ctatgtctac tgcccaacaa cttctcgcga ctctagccat
    48241 aggaagcttg agtaatttcg atttgatatc ctcatgtaac catgtaagtt ggagatgctt
    48301 tatttgaact tactctatag taactagtga taattcgctt tactttcttc tagcaatttc
    48361 taaaagtcga cttcattgtt cttcagatta atagtgtgag ctatctcgtg agacgtaagc
    48421 aaatttccat tctacttttt cccaattttt tcatatttat ggtgttgcac ctatttgttt
    48481 tccaatttgc ttttcaaata cattgctatg ata
//
LOCUS       DACCIQ010000014        44723 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCIQ010000014 DACCIQ010000000
VERSION     DACCIQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.014"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            103..495
                     /locus_tag="IVM62_000795"
     CDS             103..495
                     /locus_tag="IVM62_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6844770.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            523..1863
                     /gene="glnA"
                     /locus_tag="IVM62_000796"
     CDS             523..1863
                     /gene="glnA"
                     /locus_tag="IVM62_000796"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP6844771.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3056..4666
                     /locus_tag="IVM62_000797"
     CDS             3056..4666
                     /locus_tag="IVM62_000797"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP6844772.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            4958..5827
                     /locus_tag="IVM62_000798"
     CDS             4958..5827
                     /locus_tag="IVM62_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP6844773.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5992..7263
                     /locus_tag="IVM62_000799"
     CDS             5992..7263
                     /locus_tag="IVM62_000799"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6844774.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7260..8555
                     /locus_tag="IVM62_000800"
     CDS             7260..8555
                     /locus_tag="IVM62_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP6844775.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8654..8917
                     /locus_tag="IVM62_000801"
     CDS             8654..8917
                     /locus_tag="IVM62_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP6844776.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9362..9811
                     /locus_tag="IVM62_000802"
     CDS             9362..9811
                     /locus_tag="IVM62_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6844777.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9868..11286
                     /locus_tag="IVM62_000803"
     CDS             9868..11286
                     /locus_tag="IVM62_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6844778.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11286..12146
                     /locus_tag="IVM62_000804"
     CDS             11286..12146
                     /locus_tag="IVM62_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP6844779.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12320..13093
                     /locus_tag="IVM62_000805"
     CDS             12320..13093
                     /locus_tag="IVM62_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6844780.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13240..13605
                     /locus_tag="IVM62_000806"
     CDS             13240..13605
                     /locus_tag="IVM62_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP6844781.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13753..14961)
                     /locus_tag="IVM62_000807"
     CDS             complement(13753..14961)
                     /locus_tag="IVM62_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6844782.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14980..15258)
                     /locus_tag="IVM62_000808"
     CDS             complement(14980..15258)
                     /locus_tag="IVM62_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844783.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15451..16107
                     /locus_tag="IVM62_000809"
     CDS             15451..16107
                     /locus_tag="IVM62_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6844784.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16549..17346)
                     /locus_tag="IVM62_000810"
     CDS             complement(16549..17346)
                     /locus_tag="IVM62_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP6844785.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17343..18527)
                     /locus_tag="IVM62_000811"
     CDS             complement(17343..18527)
                     /locus_tag="IVM62_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6844786.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18552..19799)
                     /locus_tag="IVM62_000812"
     CDS             complement(18552..19799)
                     /locus_tag="IVM62_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP6844787.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20014..21759
                     /gene="dnaX"
                     /locus_tag="IVM62_000813"
     CDS             20014..21759
                     /gene="dnaX"
                     /locus_tag="IVM62_000813"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP6844788.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21779..22093
                     /locus_tag="IVM62_000814"
     CDS             21779..22093
                     /locus_tag="IVM62_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP6844789.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22118..22714
                     /gene="recR"
                     /locus_tag="IVM62_000815"
     CDS             22118..22714
                     /gene="recR"
                     /locus_tag="IVM62_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP6844790.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22778..23422
                     /locus_tag="IVM62_000816"
     CDS             22778..23422
                     /locus_tag="IVM62_000816"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP6844791.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23437..23766
                     /locus_tag="IVM62_000817"
     CDS             23437..23766
                     /locus_tag="IVM62_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844792.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23780..24718
                     /gene="holB"
                     /locus_tag="IVM62_000818"
     CDS             23780..24718
                     /gene="holB"
                     /locus_tag="IVM62_000818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP6844793.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24754..25578
                     /locus_tag="IVM62_000819"
     CDS             24754..25578
                     /locus_tag="IVM62_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP6844794.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25571..25918
                     /locus_tag="IVM62_000820"
     CDS             25571..25918
                     /locus_tag="IVM62_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6844795.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25987..26859
                     /gene="rsmI"
                     /locus_tag="IVM62_000821"
     CDS             25987..26859
                     /gene="rsmI"
                     /locus_tag="IVM62_000821"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP6844796.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26968..28089
                     /gene="dinB"
                     /locus_tag="IVM62_000822"
     CDS             26968..28089
                     /gene="dinB"
                     /locus_tag="IVM62_000822"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP6844797.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28143..28745)
                     /gene="nrdG"
                     /locus_tag="IVM62_000823"
     CDS             complement(28143..28745)
                     /gene="nrdG"
                     /locus_tag="IVM62_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP6844798.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29060..31249)
                     /gene="nrdD"
                     /locus_tag="IVM62_000824"
     CDS             complement(29060..31249)
                     /gene="nrdD"
                     /locus_tag="IVM62_000824"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP6844799.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31575..32342
                     /locus_tag="IVM62_000825"
     CDS             31575..32342
                     /locus_tag="IVM62_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6844800.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32332..34374
                     /locus_tag="IVM62_000826"
     CDS             32332..34374
                     /locus_tag="IVM62_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6844801.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34655..34804
                     /locus_tag="IVM62_000827"
     CDS             34655..34804
                     /locus_tag="IVM62_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP6844802.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34815..36329
                     /gene="dltA"
                     /locus_tag="IVM62_000828"
     CDS             34815..36329
                     /gene="dltA"
                     /locus_tag="IVM62_000828"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP6844803.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36326..37531
                     /gene="dltB"
                     /locus_tag="IVM62_000829"
     CDS             36326..37531
                     /gene="dltB"
                     /locus_tag="IVM62_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP6844804.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37576..37809
                     /gene="dltC"
                     /locus_tag="IVM62_000830"
     CDS             37576..37809
                     /gene="dltC"
                     /locus_tag="IVM62_000830"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP6844805.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37812..39080
                     /gene="dltD"
                     /locus_tag="IVM62_000831"
     CDS             37812..39080
                     /gene="dltD"
                     /locus_tag="IVM62_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP6844806.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39221..40303
                     /locus_tag="IVM62_000832"
     CDS             39221..40303
                     /locus_tag="IVM62_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP6844807.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40303..41106
                     /locus_tag="IVM62_000833"
     CDS             40303..41106
                     /locus_tag="IVM62_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6844808.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41176..41916
                     /locus_tag="IVM62_000834"
     CDS             41176..41916
                     /locus_tag="IVM62_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6844809.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41927..42931
                     /locus_tag="IVM62_000835"
     CDS             41927..42931
                     /locus_tag="IVM62_000835"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP6844810.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(42976..44673)
                     /locus_tag="IVM62_000836"
     CDS             complement(42976..44673)
                     /locus_tag="IVM62_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844811.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14361 a         7815 c         9326 g        13221 t
ORIGIN      
        1 aaaaatagag atctcatgtt agaaaaccta acatatgcag ttgacaatat tttttttaat
       61 tggtacactt tagccaactt ataaaacgaa aggggacgtc tgatgggaga aaaggaattg
      121 cgccgatcta tgtccgtatt tccaattggt acagttatga agctgactga tttgtctgct
      181 cggcaaattc gctattatga agaacaagat ttagttcatc cggaacgaag cgaaggaaac
      241 cgacgtatgt attcgctgaa tgatattgat atgttgcttg agattaaaga ctatctttca
      301 gagggattga atatggctgg gatcaaacgt gtatacgaaa tggcacaggc gaaaaaacaa
      361 gaagcaaaaa acaagaagcc gttgacagat catgacgttc gacaaatttt tcgtgatgag
      421 attttggcgc aaggtgggtt aagcaaaaca ggtcattatc agtcgcaagg attgcaaaga
      481 caattctttg actgaataaa ttaaaaaagg aagagggata ccatggtaaa gaaacagata
      541 acaggtgaag aaatcaaacg aatcattgag gaagaaaacg tacgattttt acgattgatg
      601 ttcacagaca tcttaggtac gatcaaaaat gtagaagttc cagtgagcca gatcgacaaa
      661 gtattagaaa ataaaatgat gtttgacggt tcttcgattg aaggatttgt tcgaattgaa
      721 gaaagcgata tgtatttgta tccagatctc tccacttgga tgatcttccc gtgggaaagt
      781 gctcatggaa aagttgctcg tttaatttgt gatatctata atccggatgg gacaccattt
      841 gccggcgatc ctcgcgggaa tttgaaacga gcattgaaag atatgcgcga ccttggtttt
      901 acctctttca atttaggacc tgaaccagaa ttcttcttat ttaaattaga cgaagacggt
      961 gggatcacaa cgaccttgaa tgataaaggt ggttattttg attttgcacc aacagattta
     1021 ggtgaaaatt gccgccgtga catcgtgttg gaattagaaa gtctaggctt tgaagtggaa
     1081 gcttcacatc atgaagtagc gccaggacag catgaaattg actttaaata tgcagatgtg
     1141 gtagatgctt gtgataatat ccaaaccttc aagctcgttg tcaaaacgat tgcacgcaag
     1201 catggacttc acgcgacttt catgccgaaa ccgttatttg ggatcaatgg ttctggtatg
     1261 cattgcaata tgtctttgtt caatgatgaa ggaaatgttt tctatgataa agacggtgaa
     1321 ttggaactaa gtgaaacagc ttatcatttc ttaggtggtt tgctgaaaca tgctcgtgcc
     1381 tacacagccg tttgcaatcc aacagtcaat tcttataaac gtctcgttcc aggttatgaa
     1441 gcgcctgttt atgtcgcatg gagtggccgt aatcgttcgc cattagtacg tgtaccagaa
     1501 tctcgcggtt tatccacacg tttggaattg cggtcagttg atccatctgc aaacccatac
     1561 ttggcaatgg cagtattgtt gcaagcagga ctagacggta tccgtaatca gatcacaccg
     1621 ccagcagctg ttgaccgtaa catttatgta atggatgagg aagaaagaga agctgcacat
     1681 atccaggact taccttcaac tatccataat gctatcaaag aattgcgtaa ggataatgta
     1741 atgattgacg cattaggaca acatatcttc tcaaattttg ttgaagcaaa acgtctcgaa
     1801 tgggcggcat tcagacaaac tgtttccgag tgggaaagag aacaatatct agaactttat
     1861 taaaacagga ataccgtgaa ttagaggctg tgaaatcagc gttttgacct gagtattaga
     1921 gaaactaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     1981 agctgattga acaccgttta gtggagaggt tgtgaaaaag ctgccctgca acaagtaata
     2041 agaatagcca agcaaaaaca gcttttcttg tttttgaatt ggctgaactt attaccgcag
     2101 atgcaagctt ttgaacaccg tttaccaaga ggttttgaaa aagacgttta gtttcgagta
     2161 ttaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg
     2221 agaaactggc ttttgaatac cgtttatcca gaggttgtga gcctaccatt taactccgaa
     2281 tcacaagaaa taatttcaaa aacagcttct catgtttgat ggatattgtg acttgtgacc
     2341 gaggagttgg caggagaaca ccgtttattt agaaataagg aggcacgact tttgtcgcat
     2401 ctccttattt tttacttatg tgattcgtta ccttcaatga atccctacaa ggcacttttg
     2461 ttgaaaaaaa ggtcatcttt gaaaaaatta gtggaattgt tttgtttttg atattattta
     2521 atagaaaaga tatgccaaaa atccgagaac tgtggactac gttttttacg taaaggaggt
     2581 aaataagggt gaaagaaaaa gattgtttct acgataagct atataaactg ttgctgcaag
     2641 taggaaaaag tagttctttg ttaggtggga tattatctat cataatgttt gttttatttt
     2701 tttatctatt atttcactta aaatttttca gtaacgattt tctgatttgc ttaggtttaa
     2761 cgttagctat gtttatatca agcttattat gtgggttaaa ttttttttgc actcataaag
     2821 gacttaagaa ataatttctc aaaggatatc gaaaaagaaa aaattgtaag cggatagtat
     2881 tcatttgagt acttgacttt cccttagtca gcttgtatta tacaccttgg gcaccctttt
     2941 agagggtcag aagtttacag aaatttcggc tccctattct tattgaagaa tagggagctt
     3001 tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg aacttatgac
     3061 aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga ttgttgcagc
     3121 ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa aatttgatcc
     3181 atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag tattcgtaac
     3241 agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta ttgacatcaa
     3301 tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt tgcgtaaaga
     3361 acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta caaatgagat
     3421 caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca ttacagaagt
     3481 agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc gtcaaatgaa
     3541 agcagatgtc ggtacagata atgttatgta tatccataca acattgattc cttacttgaa
     3601 agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc tacgtggttt
     3661 aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc aaagcgtgaa
     3721 aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt cgccagatgt
     3781 agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc aaattgtttg
     3841 cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag aattagaaga
     3901 acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat atgtcgagtt
     3961 accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg cctataattc
     4021 agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg tagaagaacg
     4081 tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg gagtagaagg
     4141 gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag gtatttgtct
     4201 tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag aagatgcgtc
     4261 atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg ctgatcagga
     4321 aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta aaatcaaaaa
     4381 aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac gtcaccgtca
     4441 ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc tagtcttttc
     4501 aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga aaaaattctt
     4561 tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc aaaaattaat
     4621 caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt tgaacttgat
     4681 agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt ttctcttttt
     4741 ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt tcgattaaag
     4801 cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt ttcattgatt
     4861 ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga attgtcttaa
     4921 caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat caggagctga
     4981 atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca caaacaactt
     5041 ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag tacttatcca
     5101 aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag atttaatcac
     5161 tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag accatggtga
     5221 ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt tcgatggttc
     5281 acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa aagcacatgc
     5341 aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag acggaatcat
     5401 cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta caggtatcga
     5461 ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact ggaaaggatt
     5521 agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc ctttagtatt
     5581 acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa tgggtatctc
     5641 aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc gtaaatatat
     5701 cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat tagctccagg
     5761 taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt cagctaacaa
     5821 agcgtaagca tccacataac agatacgata tataggaatg gagaacgact tttgaagtcg
     5881 ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga
     5941 taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa
     6001 atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat
     6061 agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt
     6121 gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact
     6181 gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg
     6241 ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt
     6301 aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt
     6361 gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc
     6421 atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt
     6481 tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta
     6541 attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg
     6601 ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta
     6661 catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta
     6721 gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc
     6781 tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa
     6841 gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt
     6901 gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa
     6961 atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc
     7021 gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc
     7081 acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg
     7141 gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt
     7201 atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa
     7261 tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt
     7321 atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag
     7381 ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga atattggata
     7441 tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag
     7501 atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg
     7561 caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa
     7621 gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac
     7681 ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa
     7741 tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa
     7801 aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg
     7861 aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac
     7921 gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc
     7981 gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg
     8041 ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa
     8101 gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt
     8161 tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag
     8221 tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc
     8281 tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca
     8341 aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt
     8401 ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct
     8461 ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg
     8521 gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta
     8581 agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag
     8641 gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga
     8701 ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga
     8761 atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc
     8821 attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa
     8881 aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt
     8941 ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt
     9001 cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat
     9061 agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt
     9121 cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt
     9181 ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata
     9241 cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg
     9301 atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt
     9361 tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc
     9421 aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa
     9481 tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga
     9541 agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt
     9601 ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat
     9661 ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact
     9721 ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc
     9781 agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga
     9841 tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact
     9901 ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac
     9961 catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa
    10021 atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt
    10081 gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta
    10141 atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct
    10201 gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt
    10261 ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg
    10321 gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga
    10381 gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct
    10441 ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca
    10501 ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca
    10561 aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt
    10621 ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg
    10681 gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc
    10741 ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact
    10801 tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg
    10861 gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa
    10921 tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt
    10981 acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg
    11041 acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct
    11101 catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc
    11161 ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca
    11221 gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa
    11281 gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag
    11341 taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga
    11401 gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg
    11461 agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact
    11521 tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg
    11581 tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg
    11641 ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata
    11701 atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga
    11761 tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct
    11821 tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac
    11881 aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg
    11941 aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt
    12001 tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt
    12061 atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca
    12121 cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat
    12181 agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt
    12241 cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata
    12301 aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa
    12361 ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag
    12421 cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt
    12481 tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat
    12541 caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat
    12601 tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa
    12661 cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt
    12721 gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc
    12781 aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat
    12841 aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc
    12901 aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca
    12961 aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt
    13021 gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg
    13081 tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac
    13141 cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga
    13201 taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg
    13261 gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt
    13321 gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta
    13381 ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt
    13441 tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg
    13501 atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg
    13561 ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact
    13621 attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata
    13681 aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc
    13741 tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg
    13801 actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc
    13861 aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc
    13921 aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg
    13981 ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag
    14041 gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa
    14101 taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc
    14161 gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa
    14221 aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac
    14281 acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc
    14341 aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat
    14401 attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa
    14461 ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac
    14521 agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc
    14581 agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc
    14641 attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt
    14701 tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa
    14761 ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa
    14821 acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat
    14881 caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt
    14941 attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa
    15001 tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg
    15061 aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg
    15121 attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac
    15181 taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt
    15241 gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat
    15301 aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt
    15361 taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt
    15421 ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg
    15481 gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg
    15541 atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca
    15601 aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta
    15661 aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat
    15721 gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag
    15781 ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg
    15841 cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat
    15901 gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa
    15961 gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa
    16021 ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac
    16081 ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac
    16141 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta
    16201 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac
    16261 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    16321 tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct
    16381 gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga
    16441 cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac
    16501 acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct
    16561 gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct
    16621 tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc
    16681 ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga
    16741 tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa
    16801 aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt
    16861 ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt
    16921 agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc
    16981 atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga
    17041 aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc
    17101 gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt
    17161 cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa
    17221 agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc
    17281 atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt
    17341 catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga
    17401 gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt
    17461 ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc
    17521 cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca
    17581 ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt
    17641 aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg
    17701 aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg
    17761 ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa
    17821 gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct
    17881 atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct
    17941 gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa
    18001 gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct
    18061 ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat
    18121 agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg
    18181 atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat
    18241 tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc
    18301 atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct
    18361 ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct
    18421 tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa
    18481 gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct
    18541 ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat
    18601 ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc
    18661 ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa
    18721 ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga
    18781 ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga
    18841 taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct
    18901 tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta tccttcacgt
    18961 acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca
    19021 tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc
    19081 caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca
    19141 taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg
    19201 aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg
    19261 attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc
    19321 cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg
    19381 aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg
    19441 acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg
    19501 aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg
    19561 atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt
    19621 ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg
    19681 actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct
    19741 agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata
    19801 aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata
    19861 ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt
    19921 aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag
    19981 agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc
    20041 tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa
    20101 aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc
    20161 ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac
    20221 ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat
    20281 gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat
    20341 cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat
    20401 atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt
    20461 gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg
    20521 actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt
    20581 atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct
    20641 gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa
    20701 ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg
    20761 gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac
    20821 caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc
    20881 cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc
    20941 agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata
    21001 aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg
    21061 gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta
    21121 gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac
    21181 gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca gccggtacag
    21241 caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa
    21301 gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg
    21361 ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct
    21421 ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat
    21481 gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg
    21541 cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg
    21601 caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc
    21661 gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa
    21721 ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat
    21781 gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga
    21841 aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga
    21901 attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga
    21961 agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga
    22021 tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt
    22081 gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc
    22141 gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact
    22201 cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt
    22261 aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc
    22321 ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc
    22381 gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca
    22441 tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt
    22501 gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga
    22561 gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac
    22621 tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt
    22681 attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat
    22741 acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat
    22801 cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa
    22861 agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga
    22921 agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc
    22981 actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc
    23041 aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc
    23101 aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac
    23161 tacgccggat tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa
    23221 gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg
    23281 tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga
    23341 tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta
    23401 tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc
    23461 caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca
    23521 aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt
    23581 gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag
    23641 caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca
    23701 gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa
    23761 ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt
    23821 gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga
    23881 aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg
    23941 tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa
    24001 gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca
    24061 gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt
    24121 tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt
    24181 ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct
    24241 ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact
    24301 gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt
    24361 aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa
    24421 ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta
    24481 tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc
    24541 tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg
    24601 caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc agaaactacg
    24661 cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct
    24721 tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat
    24781 agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac
    24841 aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa
    24901 gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa
    24961 gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca
    25021 aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt
    25081 gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg
    25141 gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc
    25201 gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct
    25261 tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg
    25321 aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc
    25381 gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct
    25441 gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg
    25501 actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta
    25561 ggtgagatga atggataaga gatcattata cgacggattg aatcaattag aatcagagtt
    25621 gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa
    25681 aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca
    25741 agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct
    25801 tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga
    25861 caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc
    25921 aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg
    25981 tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt
    26041 gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa
    26101 gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt
    26161 gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa
    26221 ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct
    26281 tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg
    26341 atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct
    26401 caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg
    26461 aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta tcggatcact
    26521 gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa
    26581 ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc
    26641 gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg
    26701 ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc
    26761 gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa
    26821 aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa
    26881 tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg
    26941 ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat
    27001 acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga
    27061 gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt
    27121 gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca
    27181 atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat
    27241 cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt
    27301 gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc
    27361 aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta
    27421 acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa
    27481 aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg
    27541 ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg
    27601 atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga
    27661 aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt
    27721 acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa
    27781 gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga
    27841 gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg
    27901 accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca
    27961 agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact
    28021 ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa
    28081 gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt
    28141 ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt
    28201 tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg
    28261 ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca
    28321 aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca
    28381 aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt
    28441 cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat
    28501 ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat
    28561 ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag
    28621 cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg
    28681 tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt
    28741 ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta
    28801 ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt
    28861 attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact
    28921 tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc
    28981 ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca
    29041 ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc
    29101 ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta
    29161 catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg
    29221 gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa
    29281 gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt
    29341 ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt ggataatcct
    29401 tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt
    29461 ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac
    29521 ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat
    29581 attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag
    29641 cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca
    29701 atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat
    29761 cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg
    29821 gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc
    29881 ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg
    29941 gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt
    30001 cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta
    30061 attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca
    30121 atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca
    30181 aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc
    30241 gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc
    30301 ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca
    30361 aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc
    30421 gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg
    30481 caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg
    30541 ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt
    30601 cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc
    30661 agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt
    30721 tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat tttccaacga
    30781 tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt
    30841 tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt
    30901 tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt
    30961 cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt
    31021 cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat
    31081 aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga
    31141 tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg
    31201 tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact
    31261 cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg
    31321 gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat
    31381 tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt
    31441 ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac
    31501 ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa
    31561 gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca
    31621 gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt
    31681 ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca
    31741 cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga
    31801 aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca
    31861 atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga
    31921 tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag
    31981 aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg
    32041 ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg
    32101 actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag
    32161 caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat
    32221 ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct
    32281 ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat
    32341 aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt
    32401 ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta
    32461 gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc
    32521 attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg
    32581 caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata
    32641 tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt
    32701 attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact
    32761 agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc
    32821 cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt
    32881 tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta
    32941 ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg
    33001 gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt cttttttcct
    33061 tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg
    33121 ttatttttga tcatattgag taaatggaag atttattact atggtatcaa ttttttgata
    33181 atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc
    33241 attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc
    33301 taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa
    33361 atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat
    33421 aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat
    33481 ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt
    33541 tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat
    33601 atttcattat atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa
    33661 atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta
    33721 gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta
    33781 tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg
    33841 ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc
    33901 acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt
    33961 ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat
    34021 atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca
    34081 gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg
    34141 gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt
    34201 ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat
    34261 ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat
    34321 cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg
    34381 gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg
    34441 ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa
    34501 ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact
    34561 gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta
    34621 cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa
    34681 attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt
    34741 cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt
    34801 ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa
    34861 atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac
    34921 aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg
    34981 tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg
    35041 gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc
    35101 gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg
    35161 ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc
    35221 ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg
    35281 gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga
    35341 tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg
    35401 atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa
    35461 ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga
    35521 atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg
    35581 gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa
    35641 ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa
    35701 cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg
    35761 accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta
    35821 aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag
    35881 gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa
    35941 gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac
    36001 gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt
    36061 atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa
    36121 aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc
    36181 agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc
    36241 agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa
    36301 aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc
    36361 gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg
    36421 ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt
    36481 tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat
    36541 acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc
    36601 cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca
    36661 tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca
    36721 aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca
    36781 gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga
    36841 aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt
    36901 ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt
    36961 actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt
    37021 aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt
    37081 tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt
    37141 tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt
    37201 ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag
    37261 caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca
    37321 tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac
    37381 agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac
    37441 gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc
    37501 aggattttta gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca
    37561 aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca
    37621 gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta
    37681 gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct
    37741 gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc aactgcattg
    37801 aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg
    37861 gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga
    37921 ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt
    37981 atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca
    38041 gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc
    38101 tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg
    38161 ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg
    38221 taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt
    38281 caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa
    38341 aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac
    38401 ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg
    38461 aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac
    38521 gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac
    38581 gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag
    38641 cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat
    38701 tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca
    38761 tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac
    38821 aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg
    38881 attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga
    38941 aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg
    39001 ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag
    39061 ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt
    39121 tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat
    39181 ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca
    39241 gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga
    39301 agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag
    39361 tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga
    39421 cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct
    39481 tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg
    39541 gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag
    39601 aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga
    39661 agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg
    39721 aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt
    39781 ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg
    39841 aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa
    39901 tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc
    39961 aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat
    40021 gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc
    40081 attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag
    40141 agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc
    40201 agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg
    40261 gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct
    40321 atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact
    40381 tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc
    40441 agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt cgataattta
    40501 cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc
    40561 agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt
    40621 ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc
    40681 atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta
    40741 cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg
    40801 atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca
    40861 tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt
    40921 ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat
    40981 ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg
    41041 tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt
    41101 acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt
    41161 gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa
    41221 aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca
    41281 ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg
    41341 atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg
    41401 caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat
    41461 tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa
    41521 gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta
    41581 gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt
    41641 gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca
    41701 gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct
    41761 ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt
    41821 ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata
    41881 gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc
    41941 aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat
    42001 gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag
    42061 aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt
    42121 tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag
    42181 ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat
    42241 catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct
    42301 agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt
    42361 tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt
    42421 aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat
    42481 caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat
    42541 cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga
    42601 tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa
    42661 ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat
    42721 tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt
    42781 cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa
    42841 agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga
    42901 accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat
    42961 ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg
    43021 atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt
    43081 catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa
    43141 taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc
    43201 gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa
    43261 ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc
    43321 ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta
    43381 acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt
    43441 ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat
    43501 taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg
    43561 gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa
    43621 atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat
    43681 aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat
    43741 acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg
    43801 tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt
    43861 gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc
    43921 aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa
    43981 aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc
    44041 tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat
    44101 accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa
    44161 gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa
    44221 ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga
    44281 ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg gcagtccagc
    44341 atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg
    44401 taaatctatc acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg
    44461 gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa
    44521 atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc
    44581 cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt
    44641 atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat
    44701 aaaataaaag aaataagcaa ttc
//
LOCUS       DACCIQ010000015        42524 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCIQ010000015 DACCIQ010000000
VERSION     DACCIQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.015"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            252..941
                     /locus_tag="IVM62_000837"
     CDS             252..941
                     /locus_tag="IVM62_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6844812.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            928..2136
                     /locus_tag="IVM62_000838"
     CDS             928..2136
                     /locus_tag="IVM62_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6844813.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2430..3701
                     /gene="serS"
                     /locus_tag="IVM62_000839"
     CDS             2430..3701
                     /gene="serS"
                     /locus_tag="IVM62_000839"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP6844814.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4250..5734)
                     /gene="guaB"
                     /locus_tag="IVM62_000840"
     CDS             complement(4250..5734)
                     /gene="guaB"
                     /locus_tag="IVM62_000840"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP6844815.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6239..6934)
                     /locus_tag="IVM62_000841"
     CDS             complement(6239..6934)
                     /locus_tag="IVM62_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP6844816.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7024..8124)
                     /gene="ychF"
                     /locus_tag="IVM62_000842"
     CDS             complement(7024..8124)
                     /gene="ychF"
                     /locus_tag="IVM62_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP6844817.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8133..8324)
                     /locus_tag="IVM62_000843"
     CDS             complement(8133..8324)
                     /locus_tag="IVM62_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP6844818.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8345..9235)
                     /locus_tag="IVM62_000844"
     CDS             complement(8345..9235)
                     /locus_tag="IVM62_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP6844819.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9222..9989)
                     /locus_tag="IVM62_000845"
     CDS             complement(9222..9989)
                     /locus_tag="IVM62_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6844820.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10001..10714)
                     /gene="rsmG"
                     /locus_tag="IVM62_000846"
     CDS             complement(10001..10714)
                     /gene="rsmG"
                     /locus_tag="IVM62_000846"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP6844821.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10946..12847)
                     /gene="mnmG"
                     /locus_tag="IVM62_000847"
     CDS             complement(10946..12847)
                     /gene="mnmG"
                     /locus_tag="IVM62_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP6844822.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12864..14261)
                     /gene="mnmE"
                     /locus_tag="IVM62_000848"
     CDS             complement(12864..14261)
                     /gene="mnmE"
                     /locus_tag="IVM62_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP6844823.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14605..14943
                     /locus_tag="IVM62_000849"
     CDS             14605..14943
                     /locus_tag="IVM62_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844824.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15111..15869)
                     /locus_tag="IVM62_000850"
     CDS             complement(15111..15869)
                     /locus_tag="IVM62_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6844825.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15887..16705)
                     /locus_tag="IVM62_000851"
     CDS             complement(15887..16705)
                     /locus_tag="IVM62_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6844826.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16702..17058)
                     /gene="rnpA"
                     /locus_tag="IVM62_000852"
     CDS             complement(16702..17058)
                     /gene="rnpA"
                     /locus_tag="IVM62_000852"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP6844827.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17176..17310)
                     /gene="rpmH"
                     /locus_tag="IVM62_000853"
     CDS             complement(17176..17310)
                     /gene="rpmH"
                     /locus_tag="IVM62_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP6844828.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17833..19167
                     /gene="dnaA"
                     /locus_tag="IVM62_000854"
     CDS             17833..19167
                     /gene="dnaA"
                     /locus_tag="IVM62_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP6844829.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19365..20495
                     /gene="dnaN"
                     /locus_tag="IVM62_000855"
     CDS             19365..20495
                     /gene="dnaN"
                     /locus_tag="IVM62_000855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP6844830.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20724..20966
                     /gene="yaaA"
                     /locus_tag="IVM62_000856"
     CDS             20724..20966
                     /gene="yaaA"
                     /locus_tag="IVM62_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP6844831.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20953..22077
                     /gene="recF"
                     /locus_tag="IVM62_000857"
     CDS             20953..22077
                     /gene="recF"
                     /locus_tag="IVM62_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP6844832.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22074..24020
                     /gene="gyrB"
                     /locus_tag="IVM62_000858"
     CDS             22074..24020
                     /gene="gyrB"
                     /locus_tag="IVM62_000858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP6844833.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24040..26511
                     /gene="gyrA"
                     /locus_tag="IVM62_000859"
     CDS             24040..26511
                     /gene="gyrA"
                     /locus_tag="IVM62_000859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP6844834.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26747..27046
                     /locus_tag="IVM62_000860"
     CDS             26747..27046
                     /locus_tag="IVM62_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP6844835.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27096..27608
                     /gene="ssb"
                     /locus_tag="IVM62_000861"
     CDS             27096..27608
                     /gene="ssb"
                     /locus_tag="IVM62_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6844836.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27634..27870
                     /gene="rpsR"
                     /locus_tag="IVM62_000862"
     CDS             27634..27870
                     /gene="rpsR"
                     /locus_tag="IVM62_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP6844837.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28013..29986
                     /locus_tag="IVM62_000863"
     CDS             28013..29986
                     /locus_tag="IVM62_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP6844838.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            29992..30444
                     /gene="rplI"
                     /locus_tag="IVM62_000864"
     CDS             29992..30444
                     /gene="rplI"
                     /locus_tag="IVM62_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP6844839.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30718..32085
                     /gene="dnaB"
                     /locus_tag="IVM62_000865"
     CDS             30718..32085
                     /gene="dnaB"
                     /locus_tag="IVM62_000865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP6844840.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32865..34157
                     /locus_tag="IVM62_000866"
     CDS             32865..34157
                     /locus_tag="IVM62_000866"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP6844841.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            34944..35807
                     /locus_tag="IVM62_000867"
     CDS             34944..35807
                     /locus_tag="IVM62_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6844842.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(35936..37558)
                     /locus_tag="IVM62_000868"
     CDS             complement(35936..37558)
                     /locus_tag="IVM62_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6844843.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37807..38328
                     /locus_tag="IVM62_000869"
     CDS             37807..38328
                     /locus_tag="IVM62_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844844.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38514..40244)
                     /gene="spxB"
                     /locus_tag="IVM62_000870"
     CDS             complement(38514..40244)
                     /gene="spxB"
                     /locus_tag="IVM62_000870"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP6844845.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40409..41227
                     /locus_tag="IVM62_000871"
     CDS             40409..41227
                     /locus_tag="IVM62_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP6844846.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41330..41527)
                     /locus_tag="IVM62_000872"
     CDS             complement(41330..41527)
                     /locus_tag="IVM62_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844847.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41696..42274
                     /locus_tag="IVM62_000873"
     CDS             41696..42274
                     /locus_tag="IVM62_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6844848.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        12911 a         8182 c         8426 g        13005 t
ORIGIN      
        1 gaaaatgctt atttatcaac atttctagct tacatgatca ttggttagtt ctggtccttt
       61 ttcgttcgtc tttataactc cgagagacgt aaaatccttt tgttttcagt ttaatgttca
      121 tgttcttgtc ttattcacac caactagatg tctcaccttc taaaattttc cttaacaaag
      181 tgctgctttt atctatttct gctttttttt gctagaataa tagtaacaat atgactctgg
      241 aggatttatt tatgaaaatt ttgattgctg acgatgataa agaaatcgtt gaattattaa
      301 gtatctatgt tcacaatgag gggtatgaag cagtaaaggc ttatgatggg aaagaagcat
      361 tatctaaaat cagaacaatc cctgatattg aattacttat tttagatatc atgatgcctg
      421 agatggatgg gatgcaagta gtgaaagagc tacgaaaaga atcccaaata ccaatcatta
      481 tgctgacagc aaaaactact gatatggata agatcaaagg actggtcgct ggtgcagatg
      541 attatgtgac caagccattt aatccgttag aagtaatggc tagagtaaag tctttattac
      601 ggcgtacaaa aatgcagatt gccccagatc agcctgatgt attagaagta aatgcattaa
      661 tcatcaataa agactctcat gaagttaaaa caatcgatgg gaaagaaatc cagttaacag
      721 ctttggaatt cggtatcctc tacttgcttg ctagtcatcc taatcgagta tttagcgcgg
      781 atgaaatttt tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa actgtcatgg
      841 ttcacgttag tcatttaaga gataagattg aagaagcaac tggtggtgaa aaagtcattc
      901 aaaccgtttg gggagtgggc tataaaattg acgcaagata gtaaacgagc aactgaaaaa
      961 cgccgtcgga tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc
     1021 acgattatcc ttttgcttct attaaatgtt tctattctgg ttgttatcag ttcggtgatc
     1081 aacagttcac cttctttgac gaatgcgatt tgggattcaa agaatatttt tgcagaacga
     1141 ttgaacactg atttgttctg gaacggccgg aattttatta ttcccttttt ctttctatta
     1201 gatattggtg ttttgtattg gcgcttgatt cgaagatatc gccaaatgca attgcggcat
     1261 atcatcagcg aacttcacta catcgcaaac ggaaattatg atcatcgtat cccttttgaa
     1321 ctgagtggtg acttaagccg agtggtaaca agtatcaatg gattggtgga tagtaccgta
     1381 gcagcgattg aagacgaacg taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc
     1441 agtcatgata tccgtacccc actcacgtcc atcattggat acctaggact gatagaagat
     1501 ggccagtatc actcagaaga agatctttta aagtatacgc atactgctta tatcaaagca
     1561 aagcagatga aatctttagt ggacgactta tttgaataca ctaaggtacg ccagcctgct
     1621 gtcccggtga atttttctgc ctttgacatg atccagttga tcgaacagtt agcagctgat
     1681 tttgagttgg aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa agtagactcc
     1741 cttatcatgg atggcgatac cgaaaaactt gtacgtgtct tcaataatct tttgacgaat
     1801 gcgttgaaat acggaaaagg agcaacgaag atcgtaatcg aagtagaacg tatcggttca
     1861 gaagttgttg caacagtcaa aaataacgga gcaatgatcc cacaacaggc aatcgataat
     1921 ctattcgacc gtttttatcg tgtagaagaa tctcgttcac aagcaactgg aggaactgga
     1981 cttggtttag caatcgctca aagcattgtg gctttgcacg gcggatacat ttatgcaaaa
     2041 tcagataagc aatggacttc tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta
     2101 cctattgata cacaggaagt tattgacgaa agctgaaata ttcgctattc ttattacaat
     2161 tattagaata aatgaaaaac acagaaccca gtacacttta ttctttttca gagagctgat
     2221 ggcaggtgta aatcagcaaa ggataatttg gaagttccag ttcgtttacg tgccctttcg
     2281 aaataagaaa ggtcggttca acagccgtta tgtgttcaac aagtgcagct gacgctgaac
     2341 ttgggtggta ccgcgaattt cagactgatt cgtcccaaga ataatgggat ggatcagtct
     2401 ttttttattt tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg cgacaaaata
     2461 ttgaagaagt acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat
     2521 tcatggaact agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat
     2581 atcgaaatga tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg
     2641 ctaaaatcgc tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat actgaaattg
     2701 ctgaagtgga tgaaaaattg aaacaaattg caactacttt gccaaacctt ccccatgatt
     2761 ctgtgccagt tggaacagat gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac
     2821 gtacatttgc ttttgaacca aaaccacact gggaaattgc agaaaatctt gatattttag
     2881 attttgaacg tggcgcaaaa gtagccggta gccgttttgt ctattacaaa ggattaggtg
     2941 ctcgattaga acgtgctgtc tacaacttca tgcttgatca gcatatttac gaacacggct
     3001 atactgaaat gatcactcct tatatcgtca acagtaaagc gatgtttggt acgggacaat
     3061 ttccaaaatt caaagaagat gttttccaat tggagaatac tgatctaaca ttaatcccaa
     3121 cagctgaagt accattaact aactattata atgatgaaat tttagatggt gcagatttac
     3181 ctatttattt cactgcacta agccctgctt ttcgttctga agccggaagt gctggtcggg
     3241 atacacgagg actgattcgt cttcaccaat tcaataaagt agaaatggtc aaattttcgg
     3301 atgtagagca ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa gatatcttga
     3361 aaaaattgca tttgccttat cgtgtgatgg ccttatcaac tggagacatg ggcttttctg
     3421 cagctaaaac ttatgactta gaagtatgga tacctgcaca agatacttat cgtgaaatca
     3481 gttcttgttc taactgtgaa gatttccaag cacgccgcgc aatgatccgt tatcgcgata
     3541 atgatggaaa aatccactat actcatactc tgaatggttc aggacttgca gttggccgta
     3601 cagtagctgc cattttggaa aactaccaaa atgaagatgg ttctgtcaca gttccagaag
     3661 cgctcgttcc ttatatgggg aatctgaaag ttattaaata aagaggctgt gaaaaagccg
     3721 cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
     3781 tggacttaat accgagaaac tggcttttga acaccattta tcgagaggtt gtgaaatcag
     3841 cgaattaacc tgagtattag agaaaaaaat ggaaaaatag tccctcatat tttaccgttt
     3901 gttgaactaa taccgagggt cagctgattg aacaccgttt agtggagagg tcgtaagcct
     3961 accccccagc tcctagttac aaggacaaat tcaaaaaaca gctcttcata ttttatgaat
     4021 gttgtacttg tgattcggag gtggcaaggg aacactgttt attcaaaaag aggctgggac
     4081 agaagcgttt aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg
     4141 tgaatttctt cttatttccg aaggagttgc ttctacttcc gccgtttata cgtgtttaga
     4201 gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaatgtttt taagattcaa
     4261 ctgaatagtt tggtgcttct tttgtgattt gtacatcatg agggtgtgat tcttttaatc
     4321 cactgccgct catttggata aattgcgctt catcacgtaa ttgctgtaga ttggctgccc
     4381 caacataacc cattcctgaa cgcaaaccgc cgatcatttg gaaaataata tctgcaacac
     4441 ttcctttata agcaacccga ccttcgattc cttctggtac taatttattt gcttcattga
     4501 cactaccttg gaagtaacgg tcttttgaac ctttttccat cgcgcctaat gagcccatac
     4561 ctcggtatgt tttgaatcga cgtccttgga agatttcgaa ttcacctggt gattcatctg
     4621 tccctgccaa catacttcct agcatcactg catgtccgcc ggcagctaat gctttgacga
     4681 tgtcaccaga atatttgatc cctccgtctg caataatcgc tttgccatat tgacgagcaa
     4741 ctgatgctgc atcataaata gctgtgagct gaggtacacc tacaccggct accacacgtg
     4801 tcgtacaaat cgatcctggt ccgataccaa cttttacgac atctacacct gcatcatata
     4861 aagcttttgt cgcttcagcc gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag
     4921 ttgaacggat ttcttgaatt tttcggatca cacctgcact atgtccatga gctgtatcga
     4981 tgatgattgc atctgcccct gcttctaata aagcattcgc acgctcaaat gtatcgcttg
     5041 ttacaccaac ggctgctgct acaagcaatc gaccatgttc atcttttgct gcatttggaa
     5101 attcgatcac tttctcgatg tctttgatcg tgatcaatcc gcttaaacga ccttcgttat
     5161 ctacgattgg taatttttcg attttatgtt tttgcaaaat cttttctgca tcttttagag
     5221 aggtacctac tggggcagtc actaagttgt cttttgtcat cacatcagca atcggcatgt
     5281 gataatctgt tacaaaacgc atgtcgcggt ttgtaattat ccctaccaat tttctgtttt
     5341 caagtgtttc aacgattggc acaccactga tacgatattt gctcatcaat tcttctgcgt
     5401 ctgctacaag atgctgtggc gttaagaaga acggatcaat gattacgcca ctttcagaac
     5461 gtttgacttt tcttacttca tccgcttgct ggctgatcgt catgttttta tggatcacac
     5521 ctagtccgcc ttgtcttgcc atagcaatgg ccatcttgct gtcggtaaca gtatccatac
     5581 ttgcactgat gattggaata ttcaacttaa tatttttagc taattgtacg ctcatatcca
     5641 cttcgtttgg taacacgtga ctttctgctg gtatcaataa gacatcatca aaagtaaatc
     5701 cttttttcgt gaactttgtt tcccagttgg acatttttag taccactcct ctaatttagt
     5761 ttatctatta aaataattga ttttttcatt taagtcaatg atctacttgg aatcaagtta
     5821 gctttctcat agaataccaa tataaagcgt ttacatctgt gtgtattaaa agagattgta
     5881 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt
     5941 tgaattggtt gggtttatta ccgcagatgc aaacttttga acaccgttta gtggagaggc
     6001 tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
     6061 tttttgcata aattggactt aataccgaga aactggcttt tgaacaccgt ttatcgtaaa
     6121 aagggactgt gacaagactt ttgtcacagt cccttactat tatttaacta tggataaaca
     6181 aaatgaggtt ctttactacc cctagatatc tagcaaaata gctgatcttt ttgctagttt
     6241 atcttcttgg gacggataaa cttccaataa tcccgtattt tttcttcaag tagtaacgga
     6301 ttgctaaagc agttgccccg ataatgataa ctactactgg atctaatact ggattgataa
     6361 ctggtggcaa tagtgttgtc gcagaaaaca gtacgaccca aagaagtgtc gttccaccta
     6421 ggataagcaa tgtcttaccc attcctggtt tcttgcttcg atctgctccg ggatattcat
     6481 attgatagat gtatttgtac atcatataaa agacccagcc accgattgca gaacctagaa
     6541 cgagtgtaga aaagccataa gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa
     6601 tcatgatact taatagacca agcaacagca acgtattatc taaccacatc aataaagggg
     6661 tggatgaatt ttttacttct gctggcttat tgatgattgc ttccgttctt tcagagactg
     6721 tcccaaacaa ttgacgggct gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa
     6781 tgtcatgaag ggcttttact ttttcttctt cagacaaatt cgctgcttct aaagattttt
     6841 tcaaatcaaa aatgtactgt tgatttcttt tggttaattt tgtttctaaa ctattgtttt
     6901 cttttacgat tgctcgtaat tgttctggtt ccattatcta atagtcctcc ttagccgttc
     6961 cttttctcaa cggtcgtcac aaaagacatt ctaccatatt ctacagctag tttgatagcc
     7021 cttttacaca ttaaagcgga ataacattac gtcgccgtct tgtacgacat aatcttttcc
     7081 ttctaatcga acacgtccag cttcttttgc tgcatgcatg tttccgtaat gatccaagtc
     7141 ttcaaaagag acagtttctg cacggataaa tccgcgttcg aaatctgaat ggatgattcc
     7201 tgctgcttgt ggtgctttga ttcctttttt gaatgtccaa gcacgtactt cttgttcccc
     7261 agcagtaaag taagtagcta agcctaacaa atcataagca gcacggatca actgatccaa
     7321 acctgattct tcgattccta aagcttctaa aaattccgct ttgtcttctt catctaattc
     7381 tgcgatttct tcttctgcac gagcactgac cacgatcact tctgcatttt cttctgcagc
     7441 aaagttgcgc acttcttgta cataatgatt cgcgtctgca tctgctacgt catcttctga
     7501 gacattggct acataaagaa ttggttttgt cgtcaataaa aatagtgatt taacgatttt
     7561 ttgttcttct tcagtaaatt cgatcgttcg ggcagatttt ccttcttcca gtacaggttt
     7621 gattttatcc agtacagcta gttctgccac tgcatcttta tctttggttt tagcaacttt
     7681 tgctacacga gtatagcgtt tgttgataga ttctaagtct gctaacacta gttccaagtt
     7741 gatcgtatca atatcttcta gtggatcaac acgtccttct acgtgagtaa tattatcatc
     7801 atcaaaacag cgaacaacgt gacaaatagc gtctacttgg cggatatggc ttaagaattg
     7861 atttcccaaa ccttctcctt tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc
     7921 aaaagtcgtc ggaacagttt ttttcggatg tacaagttct gttaagcgtt gcaaacgcca
     7981 gtcaggtact tctaccatcc ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc
     8041 ttctgctcct gcttttgtga tcgcattaaa caaagttgat tttcctacgt tgggcaaccc
     8101 aacaattcca gcagtcaatg ccataaattt attcactctt tctctgcatt tttttcaatt
     8161 acttttttta atttcttttc aaaatctcga cgggtcatca tgacgatatg tccgcaattc
     8221 atacatctga ttttgatatc tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa
     8281 gcgtgaggtt ttttcatttc aacgatatcg tgcaagtcgt acataaaaat agcctccttt
     8341 ttgtttattc ttcatcaaat tgaatattca agatatcaag aattcgagtc aaatcttttt
     8401 gagaaagata ttcaatctcg atcttacctt tcccattttt ttcttggata gctacacttg
     8461 taccaaattt atccatcaaa cgatcttcgc tttctcggat ataatatggt ttttcctttg
     8521 ctagccttgg gatttttttc ttttcttttc catcattttg atttaactca gaaacaagct
     8581 gttccagttg gcggacagtt aaattttcag ttactgcacg attggctaat ttcaagatca
     8641 atgttttatt ttttaaacct aacagcgtac gggcttgtcc catagataaa cgctgatctt
     8701 gtaccatttc cttgacaaga tccggtaaag aaagcaagcg caaataattg gcgatataag
     8761 gtctgctctt tcctaaacga ctagctactt ctgcttgtgt tagtttcagg tttttcatca
     8821 gcatctcata tgcttctgct tcttctaacg gattcagatc ttctcgctgc aagttttcca
     8881 gcactgctac ttgcatcatc gcttcttcgt caaagtctct aacgatagca gggatcgttt
     8941 ctttttcagc tagtttggac gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt
     9001 ttactgcgga acggcgtacg atgatgggtt gaaagacacc tgattgttca atagaattag
     9061 ctagttcctg caaagatact tcttcaaatg ttttacgggg ctgatacgga tttggtcgca
     9121 gctcattcaa tggtagctgg acaacggtat catcttttac atccaccgtc tctaagtttt
     9181 ctaaatcttg aaacagtgca tcgattcctc ggcctaatcc tttacttttc ttcacgactt
     9241 accacttcct ttgccaatgc ttggtagact tcagctcctc gagaacgtgg atcgtagtcg
     9301 atgatcggtt ttccatgact aggtgcttct gataaacgaa tatttcttgg aatgatcgtt
     9361 tcgtaaactt tttcgcggaa gtatttacgt acttcttcta cgacttcatt tcccagattt
     9421 gttcgtgcat catacattgt cagtaagact ccctcgatct ctaattctgg attaaagtgt
     9481 ttttggacta aacgaaccgt attcagtaat tggcttaatc cttccaatgc gtaatactca
     9541 cactgaacag gtatcaaaat cgaatcactt gctgtaaatg aattgattgt cagatggcca
     9601 agagaaggcg ggcaatcgat caaaatataa tcatactgac tgcttacttc tgctaaagat
     9661 ccttttaatc ttgattctct tgccatcatt gacgtcaatt cgatctctgc tcctgctaat
     9721 tggagagtcg caggcacaat actcaaattt tcgtgttcag tgatcaaagt tgcctcatca
     9781 attggcaatt cattgactag aacatcatag atgtctcttg tcacatcagg ttttctgatc
     9841 ccgacaccac tggtagcatt tccttgtgca tccatatcca ctagcaatac ttttttgcca
     9901 aggctagcaa gacaagcacc tagattcacc gtcgtcgttg ttttgccgac gccacctttt
     9961 tggttcgcaa cagatattat ttgtgccatc tcttctcctc ctatttgatt ggctgcttat
    10021 taggcagtcc gggctttctt gggtatttct ttggggttgg tttctttttt tgaataacga
    10081 tgatatgccg ttgatcagag acattcggca aggtaaccgc ctcttctttg aggaatttac
    10141 cacctaataa agcaatcgct tgttttgctt ctgtcaattc ctcttcactt tttgctgctt
    10201 tcaatgcata aaaatagcct tcttgtttag ccaagggcaa acacaattcc gctaacacat
    10261 tcaaacgagc aaccgcacga gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt
    10321 gcccaaatgt ttctgcccgg tcatgatata aggaagcatc gattcctaat aatcgggtta
    10381 actcagataa gaaggttatt cgtttattca gagaatcaac gatcgttatc tgcagttctg
    10441 gaaaggcaat ttttaaaggt atgctaggaa accctgcccc cgaaccaaca tcgcataaag
    10501 ataaaggttg cgtaaagtct acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca
    10561 gatatacgtc tttttcatcc gtaatagctg taaggttcat cttttcgttc cattcgacta
    10621 atgtccgata ataaatagcg aattgattca tttgatgatc ggtaagctct atcccttttt
    10681 ctgccaagga ttggcgaaat tcttctggta tcataaatat tcctttcatt gtgaaacagc
    10741 aattgtttca cagattctct ccctacttta acgcaaaatc tctagacatg caatccttcc
    10801 atatggattc ttaaaaattt gaaaagtaag aaaagccgat agactttttc tttctagtat
    10861 gactcataaa atatacacag aagagagaac tagctttgag caatttctgc aaaaactagt
    10921 tctctcttct atttattatt acgtattacg ctggttcact gactttcgca atcttccctt
    10981 gttcgatata gaccattagg atacttaggt ctgcagggtt tacgccactg attcggctag
    11041 cttgggcaat tgtttctggt tggattttct gcaatttttg ttttgcttct gttgctaatc
    11101 cgttgattgc tgcgtagtcg atgttttctg gaatcttttt cgcttccatg cgttttaatt
    11161 tttctacttt ttccatggct tttttgatgt agccttcata tttgatttgg atttctactt
    11221 gttcgatttc ttttcgtgtc aattcttcat tttctggaat aaaccgtaga agttctgcat
    11281 aagtaatctc aggacgtctt aaaaagtcgc ttgctagtac accatctttt aatggtgccg
    11341 cattaatcgt tgctaaaaag tcttggactt cttctgttgg tttgattcgt acattcatca
    11401 aacgtttgat ttcttgttca actgcttctt ttttcttcaa ataattcttg tattgatctt
    11461 cttttactaa gcctacacgg tgacccattt ctgttaatcg caaatctgca ttatcatgac
    11521 gtaaaagcag acggtattca gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt
    11581 ttgttaccaa atcatcgatc atgacaccga tgtaaccgtc acttcgtttg atcactaaag
    11641 gttcttttcc ctgaactttc aaagctgcat tgataccggc aatcagccct tgtccagccg
    11701 cttcttcata accgcttgta ccatttgttt gtccagctgt ataaagattt tcaacgactt
    11761 tagtttctaa tgtcggacgg agctggtgtg gctctactac gtcatattcg atagcatatc
    11821 ctgtacgcat catttctact ttttctaatc cagcaataga atgaagcatg ttgacttgaa
    11881 catcctctgg taaagaagta gataaacctt gaacatagac ttcttccgta tttaagcctt
    11941 ctggttccaa aaataattga tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga
    12001 tggatggaca atagcgagcg cctacccctt caacgatccc tgtaaacatt ggggcacgat
    12061 gtaagttgtc tcgaataatt tggtgagtcg tctcacctgt ataggtcagc cagcaagatt
    12121 cctgatctag tttataggca ctatctggtg tgctgaagct aaaatgattc ggctctttgt
    12181 caccaggctg ctcttctgta acagaatagt cgatcgtact tgattttact cgaggcggtg
    12241 ttcctgtttt gaaccgttcg atagcaaagc caagttcttt tagatgattc gctagtccaa
    12301 tagaaggttg agagttattc ggacctgatg aatatttcaa ttcaccgata atgatttctc
    12361 cgcgaagtgc tgttccggct gtaataacaa ctgctttggc ccggtagctc gctcctgtgg
    12421 acgtcactac tccatgacaa acaccatctt cgacgatcag ttcttcgacg attccttgtc
    12481 ttagcgtcaa atttggttct ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt
    12541 tgtctgcttg tgctcgcaat gctcggacag ctgggccttt tcctgtgttc aacatacgca
    12601 tttggatata ggttttatcg atattcttac ccatttctcc gcctaacgca tcgatttctc
    12661 gaacgacaac gcctttagct ggtccgccta cagaaggatt acatggcatg aatgctacca
    12721 tgtccaaatt tattgtcagt aataatgttt ttgatcccat acgtgcagca gcaagtgcag
    12781 cttctgaacc ggcgtgtcct gcgccaacaa cgatcacgtc atacgcatct gcttcataat
    12841 gttccaatcg tttgttcctc cttttatttt cctaaacaaa attgactgaa taattgagtg
    12901 attagctcat cttgtacact gtcgccgaca acttctccta gatagtccca gcaacgcgtc
    12961 atatctattt gaacgagatc cacaggcatt cctgaatcaa tacctgcaat gacttcttca
    13021 agtgagaggg aagctttttc taacaacgcg atatggcggg tattagaaac ataagtagcg
    13081 tctctttctc ctgtttctcc gctgaagaat aaatcagaaa tagctgattc caaacgatct
    13141 agtccgtcgt ttttagctac ggaaattgaa aaaactggtt cattttcgat caatttcttc
    13201 aatttttctt gttccagctg tgctggcaaa tctgttttat ttagaagaat gatccgtttc
    13261 aatccacttg tcgcttccaa taattgttca tcttctgctg tcagtggttc gctttggttt
    13321 agaacaagta agatcaagtc tgcttctgct aatgcttttc ggcttctttc gaccccgatc
    13381 cgttccacca catcttctgt ttcccgaatt ccagctgtat cgattagctt gagcggtacc
    13441 ccccgaacat tgacatactc ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg
    13501 attgcttttt cttctcttaa caaatggttt aacaaacttg atttgccgac atttggccgg
    13561 ccgatgattg ccgtacttaa cccttcccgc agaaccttcc cttgcttgga tgtagcaagt
    13621 agcgcttgga ttctttgttg gatcatctgc gctttttcaa gcaataactt cgtagtcaat
    13681 tcttcgacat catcatattc tggataatca atatttactt ctacttgtgc taatgtctct
    13741 aaaatctctt gtctcaatga gcggatcaaa gcagacagat tgccgtctaa ctgattaaga
    13801 gctagtccca ttgctttgtc tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt
    13861 gaaaggtcaa cacgaccatt caagaatgct cgttttgtga attcgcctgg ctcagctaat
    13921 cttgcacctt ctcttaacaa tagctgcaaa atttgattga ctacaacgat cccaccgtga
    13981 caattgattt ctactacatc ttcccgcgta aatgtctttg gtgctctcat cacggagacc
    14041 atgacttcat cgacaagctg attgctcttg ggatcaacga tatgtccata atggatcgta
    14101 tgacttggta cttctgataa tcgtttgttt ccagattgat aaactttatc tgctagttgc
    14161 acagcttgat cgccacttaa acgaacgata ctgattgcac cttctccagg tggggtagaa
    14221 attgccgcaa tcgtatcaaa ttctaaagta atttcagcca ttttttcact tcctttttca
    14281 ttttgcgcac aaaaaaagtg cccactccac cctttatgca caataaacgg tgaacttcgc
    14341 actttgactg cttgttcttg attaaattct ggtcatagat tatcacactt gctataagga
    14401 atcaagtgtt taaaacaatt agatgaagtt gagcctgttc tgcttccatt accttatatt
    14461 taattgtcct tggatgtctc tttgcgttct ttggtttatt cgaagagaag atactgtata
    14521 aacaaggctt tatctttctt aaaaaaaacg gtacactaaa tggtagcgct atctaaacta
    14581 tacacttttg aaaggagaaa atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa
    14641 agtgaccctc ggatcaacga tgtcgtttta tctacaggga atatcaacca gaaatatatc
    14701 gtccgcgata tcgtttttgt tgtggaatgc ttagaagctg atttgtttga cccatctttt
    14761 cccaaagatg accttttctt aacaacgaag aggaaattaa agacgaaggt acttgaatat
    14821 ggtgcgaatg cagtaatcaa ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag
    14881 tattttctgg aactttttgc ttatggaaca gtgatccaat ttatccaaac aacgattggt
    14941 taaatgaaac agacaaaaaa agcggatcaa aactgaagac agtttcgatc cgcttttttc
    15001 tatcaaagtt agtgactact tcatcgtata ctctgataag aatgatttct agtaaaatat
    15061 acttggtttg aaaaactttg tcaacgtgat ttattcaaaa agtaatggaa tcaataatat
    15121 tttttagcag gttctactac taaataacga taaggttcct caccttccga atgtgttttt
    15181 acatgttcat ctttgcttaa tgcagcatgg atttgtttac gttcaaatgc aggcattggt
    15241 tctaagaaaa caggctgccc tgtccgtttt actttttcag ccgtgcgatc agctaaacgc
    15301 tgaatgatct cttggcgctt ttcacggtaa ttgcctacat tgactactac agacagctta
    15361 tttcttgcta aacgatggat gaagacttgt gccagatatt gcaatgcatt cagtgttttt
    15421 ccatgtttcc caatcagtat gccttgcttt tgtgtttcta aatggaaaac aaccatatta
    15481 tttgattctc tttccatttt gacaagagct ggagcatcaa gctgttttga aatttcagta
    15541 agataaactg ccaattccgt taaagctgct tcatcaccta gctcacttac ttcttctgtt
    15601 ttttcttcaa gaatcgtttc gttgactacc tcgatctcag ctgttggttc ttcttccaaa
    15661 atttcttcta cagtctgttc tactgtttca gaaacttttt cgctgatact tggctggatc
    15721 gaaagccgtg cattctttcg acccattcct aggaaacctt tttttccttc ttccagtatt
    15781 tcgatttcga cttggtcttt ggccaaaccc aaatcagcca gacctagttg gacagcttcg
    15841 tctattgttg cagcttcata aatcggcatt agcattacct cccctattat ttccgttttt
    15901 tcttaggatt cattgctttc ttaagagcac gttctctttc gcggattctc cgtgcttctt
    15961 cttcacgctc ttttcgaatt ttgaaagggt tgttgatcag cagcgtttgt acaacttgga
    16021 aggcattgga aactacccag tataatgaaa gaccgctggc taaattgaca cccatcacta
    16081 aaatcattac aggcatcacg aaattcatga tcttcattga tgcattcgat tcaatttgac
    16141 tcatgctaga taaatacgta cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa
    16201 atgttggatc cgcgttccct aagttcaacc ataagaaatg cccttgtttc aactctggta
    16261 cacgtgaaat agattgccat aacgccatca agattggcat ttgtactaat aaaggaagac
    16321 aaccagcata tggattgacg ccattttctg catataaacg ctgctgctct tctcttaact
    16381 tactttgtgt ttccggatct cttgaactat attgttgctg cagtgctttt aatttcggtt
    16441 gtaattcttg cgttttacgc atgctctttg tttggaaatg catcaacggc aataggatca
    16501 cgcggatgat cagtgtaaat aaaatgatcc ctatccctgc attaccaaat gacaacgcct
    16561 tgatcgcctc agcaaagtaa taaacaatat aacgatccca aattcctgta ctctgtgcgc
    16621 tgacttcccc agttccgcaa ccggataaga cgatgaccaa actaacgata ccggccatca
    16681 aaagtaaacg tttgtatttc ttcacttact cttttcctct ctgtcgatta tttttgcaag
    16741 ttttaagacg tggactaaat tggagcgaac ctcatcataa gtcaacccct tcacacttgg
    16801 tctagcaatc acaataaaat ccaactccgg atcaatttct tcttttatag aataaactgc
    16861 tgctcggatt tttcgtttga cttcattgcg gtttaccgcg ttgccgacct tttttcctac
    16921 agataaacca actcgaaaat gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt
    16981 gcggttagca aaagaatctc ccgtatggaa cacggcttga aactctcgtt ctttttttac
    17041 tcgatacgct tttctcatta gttttctttc cttttctatc tctcacttac gctcataatc
    17101 ataccataat tacgattgtc ctttcatcat ttccctaaaa aagagtataa aaaaaaccac
    17161 tgaaacccca gtggcctaag ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa
    17221 cacacgacgt ccatttttag tactcatacg tttacggaat ccgtgaactt tttgacgttt
    17281 acgtttattt ggttggtatg ttcttttcat atattccacc tccaaaatgt tcttatctat
    17341 tttataaaca cagacatact ttgatagtat aacgacaaag tagtgatttt gtcaatataa
    17401 aatagaagat cgattgttaa aacttatcca caagattatt tttttctatg ggaatcctat
    17461 gtggaaaaag ttatgcacag ggattatcca cagataataa aaggctggtt ttacacagaa
    17521 aacacacgtg taaaaaaagt aatccacaat caggcgattt cctgtgtatt ccgtctccta
    17581 actattggta tatctgtatt ttttatccac agacgcaaga ggaaatctat tggtttttac
    17641 gagttttcca cctgtgcatt agtggtgtgg aaaacttttt ttgacaactg tgtttttctc
    17701 ttttttcttt ttgtcgattg tgtggataat tgtaattaaa aaattttttt cttcggtttc
    17761 tgtggaaaac tattgaaaaa aatgctaaat tagtactagt tattcacttt ttaatgaagg
    17821 aggaggcctt atatggtatc cctcgatgct ttatggaatg aattaaaagc aacataccaa
    17881 aaggatttat cgcctgctag ctataataca tggatcgaaa cagcgaaccc tcgaaccctt
    17941 gatcaaaacc agctcgttgt tgaggttcct agcaagattc ataaagaata ttgggaaaaa
    18001 aacctggcga caaaaatcgt tgaagtcggt tatatgttat caggaaatga gattatccct
    18061 cgctttatta ctggcgaaga agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa
    18121 gttgtggcac ctagtccgct aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg
    18181 tttgtcattg gtaaaggaaa ccagatggct catgcagcag cgctcgttgt agcagaagat
    18241 ccaggttcta tttataaccc gctcttcttt tatggaggcg tgggacttgg taagactcac
    18301 ttgatgcatg caattggtca tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat
    18361 gtaagcagtg aaacttttgc taatgatttc atcaattcga tccaaaacaa aacagcagaa
    18421 gagttccgtc aagaatatcg aaatgttgat ttactgctag tggatgacat tcaatttttt
    18481 gcagaaaaag aagctacgca agaagagttt ttccatactt tcaatgcctt gtacaatgaa
    18541 gggaaacaaa tcgtactaac cagtgatcgg ttgccaaacg agattccgaa actgcaagaa
    18601 cgccttgttt ctcgctttgc atgggggctg tctgttgata ttacaccgcc ggatcttgaa
    18661 acaagaacag cgattttacg taaaaaagcg gcggctgaac gtctagagat cccagatgac
    18721 acactaagct acatcgcagg acaaattgat tctaacattc gtgaattaga aggtgcgctt
    18781 gttcgagtgc aagcttttgc tgcgatgaat agtgaagata tttcgactag tctagctgca
    18841 gacgcactga aaacgttaaa atcaggaaaa ggacatcctc aattgtcaat cctgcagatc
    18901 caagaagaag tggcaaagta ttatcacatc caattgaaag atctcaaagg aaagaaacgc
    18961 gtcaaatcta ttgttgttcc tagacaaatc gctatgtatt tggcacgtga gctcacagat
    19021 aattctttac cgaaaatcgg agctgaattc ggtggcaagg atcatacaac ggttatccat
    19081 gctcatgaaa aaatccagca gctgatggat tctagtgtca gtatgcaaaa tgaagtatca
    19141 gaaatcaaaa actttttact aaattaattt gtcgattcta tcaactgaaa atagcatgtg
    19201 gataaaagaa aaaaacgttc aaaagttata cacacttttt ccacagctgt tttttcgttt
    19261 ctctcttacg ttttttgagt tttccacaga atcaacatgc cctattactt ttattattct
    19321 ttttaactaa taataaataa acaaacattc aaaggagata catcatgaaa gttactttaa
    19381 accgagctag ctttatgcag gaattgcaaa ctgttcaacg agctatttca agcaaaacca
    19441 cgatccctat tttgacaggt gtaaaaatca cactgacaca agaaggtttg actttgacgg
    19501 ggagcaacgc tgatatatca attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata
    19561 tgcaaatcga atctactggt tccattgttt tacaagcacg tttctttagc gaaatcattc
    19621 ggagacttcc tgaagaaaca tttactttag aagttttaga aaataaacaa gtagcgatca
    19681 cttctggaaa agcgaatttt atcgtaaatg gattagatgc agataactat cctcatcttc
    19741 ctgttgtcga aagccataac cagatgaaat tacctgtaca cgtattgact aaactaatca
    19801 acgaaacagt ttttgctgtc tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt
    19861 tcattttatc tgataattct ttattagctg tagctactga ttctcaccgt ctaagtcaac
    19921 gcgtgattcc agtagaacaa gcggctgatc attttgatat tgttattcct ggaaaaagtt
    19981 tgatcgaatt atctcgctca ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg
    20041 aaaaccaagt gttgttcaaa acagaaacga tgtatttcta ttcccgtttg ctagaaggaa
    20101 actatccaga taccaatcgt ttgattcctt caagttttaa tacagaggtt gaattttctg
    20161 ttcctagctt tttagcggcg atcgaacggg cttctttact ttctcatgaa ggccgtaaca
    20221 acatcgttcg tttatctatt cgtccagatg cggttgtttt atacggaaat tcgccagaaa
    20281 tcgggaaagt agaagaaagc ttgagttata cagcaagcag tggcgatcca ttagatattt
    20341 ctttcaatcc ggattatatg aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag
    20401 tgaaatttat ctctgctatt cgtccattta cattggaacc aacggaagat ggcgttcaat
    20461 ttattcagct gattactcct gtacgtacaa attaacaatg attttcaatg ttcttgaaaa
    20521 tttaattgaa aagaaataga aaaaataaaa ttttaaacag aaggccctaa aatctctttt
    20581 aaggctttct gtttattttt gtttaaatca ccttttttag attaaatcgt cttatatgga
    20641 aaaatatatg gataaatttg ttttattgcg aaaaaaaagg tataatagag tatactttta
    20701 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
    20761 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
    20821 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
    20881 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
    20941 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
    21001 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
    21061 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
    21121 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
    21181 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
    21241 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
    21301 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
    21361 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
    21421 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
    21481 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
    21541 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
    21601 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
    21661 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
    21721 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
    21781 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
    21841 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
    21901 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
    21961 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
    22021 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
    22081 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
    22141 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
    22201 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
    22261 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
    22321 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
    22381 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
    22441 ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat
    22501 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
    22561 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
    22621 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
    22681 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
    22741 tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca
    22801 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
    22861 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
    22921 tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga
    22981 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
    23041 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
    23101 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
    23161 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg
    23221 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
    23281 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
    23341 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
    23401 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
    23461 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
    23521 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
    23581 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
    23641 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
    23701 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
    23761 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
    23821 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
    23881 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
    23941 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
    24001 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
    24061 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
    24121 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
    24181 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
    24241 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat tgcgatctat
    24301 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
    24361 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
    24421 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
    24481 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
    24541 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
    24601 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
    24661 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa
    24721 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
    24781 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
    24841 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
    24901 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
    24961 cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa
    25021 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
    25081 aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc
    25141 tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg tattgctttg
    25201 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa
    25261 gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
    25321 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
    25381 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
    25441 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
    25501 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
    25561 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
    25621 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
    25681 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
    25741 cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc
    25801 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
    25861 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
    25921 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
    25981 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
    26041 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
    26101 ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
    26161 gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
    26221 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
    26281 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
    26341 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
    26401 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
    26461 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga
    26521 ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt
    26581 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
    26641 caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata actctccttg
    26701 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
    26761 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
    26821 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
    26881 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
    26941 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
    27001 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
    27061 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
    27121 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
    27181 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
    27241 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
    27301 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
    27361 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
    27421 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
    27481 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
    27541 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
    27601 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
    27661 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
    27721 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
    27781 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
    27841 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
    27901 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
    27961 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
    28021 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
    28081 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
    28141 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
    28201 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
    28261 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
    28321 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
    28381 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
    28441 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
    28501 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
    28561 tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact
    28621 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
    28681 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
    28741 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
    28801 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
    28861 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
    28921 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
    28981 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
    29041 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
    29101 caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg
    29161 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
    29221 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
    29281 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
    29341 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc
    29401 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
    29461 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
    29521 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
    29581 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
    29641 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
    29701 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
    29761 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
    29821 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
    29881 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
    29941 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
    30001 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
    30061 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
    30121 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
    30181 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
    30241 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
    30301 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
    30361 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
    30421 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
    30481 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
    30541 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
    30601 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
    30661 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    30721 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    30781 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    30841 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    30901 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    30961 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    31021 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    31081 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    31141 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    31201 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    31261 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    31321 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    31381 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    31441 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    31501 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    31561 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    31621 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    31681 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    31741 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    31801 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    31861 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    31921 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    31981 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    32041 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    32101 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    32161 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    32221 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    32281 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    32341 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    32401 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    32461 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    32521 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    32581 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    32641 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    32701 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    32761 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    32821 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    32881 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    32941 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    33001 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    33061 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    33121 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    33181 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    33241 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    33301 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    33361 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    33421 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    33481 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    33541 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    33601 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    33661 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    33721 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    33781 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    33841 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    33901 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    33961 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    34021 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    34081 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    34141 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    34201 gtcgaagtgt ttatctatac gataataata acttgaagag gtagtgaaag agacgtttag
    34261 ccttattgcc gataggctgt ctcttgaaca ctgtttattc agaataaggg actgtgacaa
    34321 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
    34381 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
    34441 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
    34501 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
    34561 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
    34621 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
    34681 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
    34741 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    34801 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
    34861 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
    34921 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
    34981 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
    35041 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
    35101 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
    35161 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
    35221 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
    35281 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
    35341 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
    35401 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
    35461 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
    35521 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
    35581 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
    35641 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
    35701 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
    35761 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
    35821 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
    35881 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
    35941 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
    36001 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
    36061 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
    36121 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
    36181 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
    36241 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
    36301 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
    36361 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
    36421 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
    36481 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
    36541 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
    36601 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
    36661 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
    36721 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
    36781 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
    36841 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
    36901 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
    36961 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
    37021 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
    37081 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
    37141 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
    37201 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
    37261 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
    37321 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
    37381 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
    37441 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
    37501 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
    37561 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
    37621 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
    37681 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
    37741 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
    37801 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
    37861 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
    37921 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
    37981 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
    38041 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
    38101 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
    38161 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
    38221 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
    38281 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
    38341 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
    38401 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
    38461 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
    38521 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
    38581 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
    38641 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
    38701 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
    38761 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
    38821 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
    38881 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
    38941 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
    39001 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
    39061 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
    39121 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
    39181 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
    39241 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
    39301 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
    39361 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
    39421 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
    39481 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
    39541 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
    39601 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
    39661 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
    39721 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
    39781 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
    39841 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
    39901 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
    39961 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
    40021 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
    40081 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
    40141 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
    40201 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
    40261 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
    40321 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
    40381 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
    40441 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
    40501 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
    40561 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
    40621 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
    40681 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
    40741 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
    40801 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
    40861 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
    40921 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
    40981 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
    41041 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
    41101 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
    41161 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
    41221 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
    41281 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
    41341 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
    41401 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
    41461 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
    41521 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
    41581 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
    41641 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
    41701 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
    41761 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
    41821 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
    41881 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
    41941 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
    42001 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
    42061 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
    42121 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
    42181 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
    42241 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
    42301 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
    42361 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
    42421 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
    42481 cattattcac agcaataaaa aaataaacat ctttatgtaa tctg
//
LOCUS       DACCIQ010000016        42302 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCIQ010000016 DACCIQ010000000
VERSION     DACCIQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.016"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            6..245
                     /locus_tag="IVM62_000874"
     CDS             6..245
                     /locus_tag="IVM62_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844849.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="IVM62_000875"
     CDS             312..521
                     /locus_tag="IVM62_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844850.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="IVM62_000876"
     CDS             581..1252
                     /locus_tag="IVM62_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6844851.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="IVM62_000877"
     CDS             1305..3281
                     /locus_tag="IVM62_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6844852.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="IVM62_000878"
     CDS             3294..4049
                     /locus_tag="IVM62_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6844853.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="IVM62_000879"
     CDS             4046..4870
                     /locus_tag="IVM62_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844854.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5159..7270
                     /locus_tag="IVM62_000880"
     CDS             5159..7270
                     /locus_tag="IVM62_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6844855.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="IVM62_000881"
     CDS             complement(7308..9365)
                     /locus_tag="IVM62_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP6844856.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="IVM62_000882"
     CDS             complement(9479..9691)
                     /locus_tag="IVM62_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844857.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="IVM62_000883"
     CDS             9878..10345
                     /locus_tag="IVM62_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844858.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="IVM62_000884"
     CDS             10342..10584
                     /locus_tag="IVM62_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844859.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="IVM62_000885"
     CDS             10602..10904
                     /locus_tag="IVM62_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844860.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="IVM62_000886"
     CDS             10976..13030
                     /locus_tag="IVM62_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844861.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="IVM62_000887"
     CDS             13030..15642
                     /locus_tag="IVM62_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6844862.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="IVM62_000888"
     CDS             15639..18170
                     /locus_tag="IVM62_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844863.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="IVM62_000889"
     CDS             18222..18554
                     /locus_tag="IVM62_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844864.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="IVM62_000890"
     CDS             18555..19148
                     /locus_tag="IVM62_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844865.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="IVM62_000891"
     CDS             19311..21005
                     /locus_tag="IVM62_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6844866.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="IVM62_000892"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="IVM62_000892"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6844867.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="IVM62_000893"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="IVM62_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="IVM62_000894"
     CDS             24078..25283
                     /locus_tag="IVM62_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6844868.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="IVM62_000895"
     CDS             25299..25943
                     /locus_tag="IVM62_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844869.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="IVM62_000896"
     CDS             25954..26760
                     /locus_tag="IVM62_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844870.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="IVM62_000897"
     CDS             26782..27012
                     /locus_tag="IVM62_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844871.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="IVM62_000898"
     CDS             27558..27833
                     /locus_tag="IVM62_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844872.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="IVM62_000899"
     CDS             27875..28297
                     /locus_tag="IVM62_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844873.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /locus_tag="IVM62_000900"
     CDS             28316..29908
                     /locus_tag="IVM62_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844874.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="IVM62_000901"
     CDS             29920..30255
                     /locus_tag="IVM62_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844875.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="IVM62_000902"
     CDS             30402..30596
                     /locus_tag="IVM62_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844876.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="IVM62_000903"
     CDS             30628..33000
                     /locus_tag="IVM62_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6844877.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="IVM62_000904"
     CDS             33061..34521
                     /locus_tag="IVM62_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6844878.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="IVM62_000905"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="IVM62_000905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6844879.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36975..37568
                     /locus_tag="IVM62_000906"
     CDS             36975..37568
                     /locus_tag="IVM62_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6844880.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="IVM62_000907"
     CDS             37584..38483
                     /locus_tag="IVM62_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6844881.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="IVM62_000908"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="IVM62_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6844882.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="IVM62_000909"
     CDS             39337..39597
                     /locus_tag="IVM62_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844883.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="IVM62_000910"
     CDS             40038..40244
                     /locus_tag="IVM62_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6844884.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="IVM62_000911"
     CDS             40244..40495
                     /locus_tag="IVM62_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844885.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="IVM62_000912"
     CDS             40510..40947
                     /locus_tag="IVM62_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844886.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="IVM62_000913"
     CDS             40940..41647
                     /locus_tag="IVM62_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844887.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42028..42207
                     /locus_tag="IVM62_000914"
     CDS             42028..42207
                     /locus_tag="IVM62_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844888.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT        15586 a         6722 c         8665 g        11329 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatattttt ccaaggagta gagaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactttcac cgtgagtgac gttgattatc tgttctttca aaatatcaac tcgcccctca
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcaccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtca cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatggtaa ccatatattc ttttggcgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattcggtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tggacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggcgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa
    26461 gatgaagatt atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacgtcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ctctaaagcc actcgaaaag acgggtattt
    34621 tgaaatccaa gatcgattgt ttcctggtga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgaatta gattcgcccg atatgtacga tgaaaattgg aaacaatggt cattagaaca
    34741 tttgcctatt ttccctacac attatcacta tcatgttcct aaagataaga aaaagcaatt
    34801 caaggtggtg aaacagcaac ttcaatcggc cgacaccatt attattgcga cagatagcga
    34861 tcgtgaaggg gaactgatcg cttggacgat cattcaacaa gtaggcgccg atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 gcagttacga gacgcaaaag agacctatcc aaaatttgaa gaagcacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagctttt ccttaggcag agtccaaacc ccaacgcttt acatgattta
    35161 tcaattgcag gaaaagatta gaaatttcaa gaaagaacct tattttgaag gaaaatccga
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccgaatgaaa cccaagcgac
    35281 gcaagaagca tttgaagatt atttaaaaga aaagggggtt caattgggaa aacagccagg
    35341 catgattcgc caagttgaaa ccgagaaaaa aagcgcggca agtccacgtc ttttttctct
    35401 atccagcctt cagtctaaaa tgaatcagct catgaaagct agtgccaaag acactttgga
    35461 agccatgcaa ggcttgtatg aagggaaata tctaagctat ccacgaacag acacccctta
    35521 tattacggaa ggggagtatg cgtatttgtt ggatcattta gatgaataca agcatttttt
    35581 aaaggccgaa tcaatcccaa cccctattca tacgccgaat agtcggtatg tgaataacaa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggccat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaaatata cctatgaaga aacaacgatc ttgacgcaag tccagcagct
    35821 tcagttaaag gcaatcggaa aagtaccgat agatctaggt tggaagaagt tatttggcaa
    35881 agagaacgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtagatctac aagtccttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 ca
//
LOCUS       DACCIQ010000017        38866 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCIQ010000017 DACCIQ010000000
VERSION     DACCIQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.017"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            379..1488
                     /gene="pdhA"
                     /locus_tag="IVM62_000915"
     CDS             379..1488
                     /gene="pdhA"
                     /locus_tag="IVM62_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP6844889.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1491..2468
                     /locus_tag="IVM62_000916"
     CDS             1491..2468
                     /locus_tag="IVM62_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP6844890.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2493..4136
                     /locus_tag="IVM62_000917"
     CDS             2493..4136
                     /locus_tag="IVM62_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP6844891.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4144..5550
                     /gene="lpdA"
                     /locus_tag="IVM62_000918"
     CDS             4144..5550
                     /gene="lpdA"
                     /locus_tag="IVM62_000918"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP6844892.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5712..5987
                     /locus_tag="IVM62_000919"
     CDS             5712..5987
                     /locus_tag="IVM62_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP6844893.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5993..6760
                     /locus_tag="IVM62_000920"
     CDS             5993..6760
                     /locus_tag="IVM62_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP6844894.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6928..8757
                     /gene="typA"
                     /locus_tag="IVM62_000921"
     CDS             6928..8757
                     /gene="typA"
                     /locus_tag="IVM62_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP6844895.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9018..10790
                     /gene="recQ"
                     /locus_tag="IVM62_000922"
     CDS             9018..10790
                     /gene="recQ"
                     /locus_tag="IVM62_000922"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP6844896.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10904..11206
                     /locus_tag="IVM62_000923"
     CDS             10904..11206
                     /locus_tag="IVM62_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP6844897.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11209..12372
                     /locus_tag="IVM62_000924"
     CDS             11209..12372
                     /locus_tag="IVM62_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6844898.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12398..15826
                     /locus_tag="IVM62_000925"
     CDS             12398..15826
                     /locus_tag="IVM62_000925"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP6844899.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15847..16971
                     /locus_tag="IVM62_000926"
     CDS             15847..16971
                     /locus_tag="IVM62_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844900.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16961..17398
                     /locus_tag="IVM62_000927"
     CDS             16961..17398
                     /locus_tag="IVM62_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6844901.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17382..17720
                     /locus_tag="IVM62_000928"
     CDS             17382..17720
                     /locus_tag="IVM62_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP6844902.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17839..18399
                     /gene="rsmD"
                     /locus_tag="IVM62_000929"
     CDS             17839..18399
                     /gene="rsmD"
                     /locus_tag="IVM62_000929"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP6844903.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18396..18887
                     /gene="coaD"
                     /locus_tag="IVM62_000930"
     CDS             18396..18887
                     /gene="coaD"
                     /locus_tag="IVM62_000930"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP6844904.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18877..19920
                     /locus_tag="IVM62_000931"
     CDS             18877..19920
                     /locus_tag="IVM62_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6844905.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20079..20807
                     /locus_tag="IVM62_000932"
     CDS             20079..20807
                     /locus_tag="IVM62_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP6844906.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20923..21168
                     /locus_tag="IVM62_000933"
     CDS             20923..21168
                     /locus_tag="IVM62_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6844907.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21165..21383
                     /locus_tag="IVM62_000934"
     CDS             21165..21383
                     /locus_tag="IVM62_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP6844908.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21519..22058
                     /locus_tag="IVM62_000935"
     CDS             21519..22058
                     /locus_tag="IVM62_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP6844909.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22051..24324
                     /locus_tag="IVM62_000936"
     CDS             22051..24324
                     /locus_tag="IVM62_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP6844910.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24362..25408
                     /gene="holA"
                     /locus_tag="IVM62_000937"
     CDS             24362..25408
                     /gene="holA"
                     /locus_tag="IVM62_000937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP6844911.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25497..25751)
                     /locus_tag="IVM62_000938"
     CDS             complement(25497..25751)
                     /locus_tag="IVM62_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP6844912.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26151..26420)
                     /locus_tag="IVM62_000939"
     CDS             complement(26151..26420)
                     /locus_tag="IVM62_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844913.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26890..27345)
                     /locus_tag="IVM62_000940"
     CDS             complement(26890..27345)
                     /locus_tag="IVM62_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP6844914.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27483..29411)
                     /locus_tag="IVM62_000941"
     CDS             complement(27483..29411)
                     /locus_tag="IVM62_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6844915.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29608..30441)
                     /locus_tag="IVM62_000942"
     CDS             complement(29608..30441)
                     /locus_tag="IVM62_000942"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP6844916.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(30633..31337)
                     /locus_tag="IVM62_000943"
     CDS             complement(30633..31337)
                     /locus_tag="IVM62_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6844917.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31360..31986)
                     /locus_tag="IVM62_000944"
     CDS             complement(31360..31986)
                     /locus_tag="IVM62_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6844918.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32312..34687
                     /locus_tag="IVM62_000945"
     CDS             32312..34687
                     /locus_tag="IVM62_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP6844919.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(34711..35202)
                     /locus_tag="IVM62_000946"
     CDS             complement(34711..35202)
                     /locus_tag="IVM62_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP6844920.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            35389..36309
                     /locus_tag="IVM62_000947"
     CDS             35389..36309
                     /locus_tag="IVM62_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6844921.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            36463..36807
                     /locus_tag="IVM62_000948"
     CDS             36463..36807
                     /locus_tag="IVM62_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP6844922.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(36997..37497)
                     /locus_tag="IVM62_000949"
     CDS             complement(36997..37497)
                     /locus_tag="IVM62_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6844923.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            38025..38108
                     /locus_tag="IVM62_000950"
     tRNA            38025..38108
                     /locus_tag="IVM62_000950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38059..38061,aa:Leu,seq:cag)
     gene            complement(38432..>38866)
                     /locus_tag="IVM62_000951"
     CDS             complement(38432..>38866)
                     /locus_tag="IVM62_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6844924.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        12878 a         6812 c         8049 g        11127 t
ORIGIN      
        1 gatataaaga aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa
       61 ataaatgttt gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt
      121 aagtttgatt agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga
      181 ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt
      241 tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa
      301 tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga
      361 gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta
      421 gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt
      481 aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg
      541 gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc
      601 ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat
      661 aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta
      721 cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa
      781 gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt
      841 gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat
      901 ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca
      961 aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa
     1021 actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc
     1081 gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc
     1141 agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta
     1201 tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa
     1261 gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa
     1321 gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac
     1381 aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa
     1441 tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa
     1501 aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg
     1561 tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat
     1621 tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg
     1681 caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct
     1741 ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc
     1801 gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc
     1861 atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc
     1921 gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa
     1981 gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag
     2041 tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag
     2101 atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc
     2161 ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg
     2221 tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac
     2281 aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt
     2341 cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag
     2401 cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg
     2461 aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata
     2521 tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga
     2581 tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt
     2641 cacctgttac aggaacggtt aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg
     2701 gggatgtatt agtagaaatc gatgcaccag gacacgaaga caatgaaggg gacagtggtg
     2761 tagccgcaga atcccaaact ccagctaaac cggctgctga acccactgtt gatacagaat
     2821 cagctggatc gtcaagcgaa ggagtcttcc aatttaagtt accggatatc ggtgaaggaa
     2881 tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg
     2941 atacattact agaagttcaa aatgacaaat cagtagaaga aattccgtca cctgtcacag
     3001 ggacagttaa aaacgtgatt gttccagaag gcactgtagc aaatgttggg gatgtattag
     3061 tagaaatcga tgctcctgga cacaatagtg catcgtcaac tagtgcgcca tcagctgaag
     3121 caccaaaaga aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct gccgatccaa
     3181 ataaacgtgt attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata
     3241 tctcacaagt aactgcaact ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact
     3301 tcttggcagg tggtccaagc agtgcaccag cgaaatcaga agcgccagaa gcggcagcac
     3361 cgaaagaagc agctcctgca gcagaaagca aaccagctgc accagcaaaa ccattcaagt
     3421 ccaacttagg agacttggaa gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta
     3481 aagcaatggt caacagtaag catacagctc cacacgtgac attgcatgat gaagtagaag
     3541 tgtctaaact atgggataat cggaaacgat ttaaagaagt tgctgctgca aatggtacga
     3601 aacttacttt cttgccatat gtcgtgaaag ctttaacagc gactgttaag aaatacccag
     3661 tattgaatgc atctatcgac gatgcaaacc aagaaatcgt ttataaacat tactacaata
     3721 tcggtattgc aactgatacg gatcacggac tatatgtacc aaatgtgaaa gatgcagacc
     3781 gcaaaggaat gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg
     3841 gcaaactatc tgcagaagat atgcgcaatg gaacaattac gatcagcaat atcggatctg
     3901 taggtggcgg ttggtttaca ccagtcatca actatccaga agtagctatt ttaggtgtag
     3961 gaacgattgc acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga
     4021 tgaaactgtc attaagtttc gatcaccgta tcgtagatgg agcaactgcg caacaagcaa
     4081 tgaataatat caaacgtctt ttagctgatc cagagctatt aatgatggaa ggatgataaa
     4141 caaatggtag ttggagattt tgctgtagaa ttagatacag ttgtaattgg tgcgggacct
     4201 ggtggctatg tagctgccat ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa
     4261 agagagtata ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa agcactgata
     4321 gctgcaggac accattatca agagtcacta gattctgaaa tgtttggagt aactagtgaa
     4381 aacgtcaaac tagattttgc aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg
     4441 ttgacttctg gtgttggttt cctattaaaa aaacataagg ttgaaacaat cgaaggagaa
     4501 gcgttcttcg tagatgatca cacactacgc gtcattcatc cagattcagc tcaaacttat
     4561 tcgttcaata acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa
     4621 ttcggaggac gtgtgttaga ttctactggc ggattagcat tgaaagaagt acctaagaaa
     4681 tttgtgatca tcggtggcgg tgtgattggt gccgaattgg gcggagcgta cgcaaactta
     4741 ggagcagaag taacgattct tgaaggctca ccacaaattt taccaactta cgaaaaagat
     4801 ctagtaaaat tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca
     4861 atggcgaaag aagcagtcga taacggtgac agcgtgacag tgaaatatgc agtggacggg
     4921 aaagaagaat ccgttactgc tgattatgtg atggtcactg taggtcgtcg tccgaataca
     4981 gatgatatgg gcttagaaca agcgggtgtc gaagtaggag aacgaggatt gatcacggtg
     5041 gataaacaag gacgtacgaa cgtacctaac atctatgcca tcggtgatat cgttccaggg
     5101 gcggcattag cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc
     5161 aaaaaagtag ctgtagatta taaagcaatg ccggcagtag ccttcactga tcctgaattg
     5221 gcttctgtag gtatgacgat caaagaagca aaagatgctg gtatcgaagc aaaagcatat
     5281 aaattcccat tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga aggattcatc
     5341 cgtctagtta cgacgatcga agataacgtg cttattggcg cgcaaatcgg tggagtcggc
     5401 gcaagtgata tggtctctga attagcttta gcaatcgaat caggaatgaa tgctgaagat
     5461 atcgccttga cgattcatcc tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa
     5521 ttagcattag gtttaccaat ccatatttga tttgaataat acaggcgaaa cgcaactgaa
     5581 aaatcagttg cgttttgttt tttagaatta tcaataatat tattatgtaa acttaaaaat
     5641 atgacaactt cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag
     5701 gagagttgaa gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa
     5761 tggtcattgt aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca
     5821 atgaagcttt tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg
     5881 aaaaacgatt gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc
     5941 agcaagcaaa aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca
     6001 gatgattgat catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca
     6061 acaaacatta acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg
     6121 aaacatccag aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt
     6181 ggcagaagaa gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga
     6241 tcctattgat ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc
     6301 tgtttatgaa tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact
     6361 tttttggggt ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc
     6421 aaataaatcc ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa
     6481 atatggaatc cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg
     6541 tcttgaacta gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc
     6601 ttgggattat gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct
     6661 ttttggtact cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc
     6721 atatgcagaa atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc
     6781 acaaaaatga taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact
     6841 atatttcatg aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa
     6901 gaaccaataa aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca
     6961 attattgccc acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca
     7021 gatacattag acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa
     7081 aaagaacgtg gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc
     7141 gtaaacatca tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg
     7201 aaaatggttg acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca
     7261 cgttttgtat tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag
     7321 attgacaaac cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc
     7381 gaattaggag cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat
     7441 ggtacgtcaa gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt
     7501 gatacaatta ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc
     7561 caagtttcat tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt
     7621 agaggaacaa tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag
     7681 aaattccgtg taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa
     7741 gcaaaagctg gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca
     7801 gttactcctg tggatcatca agatgcgtta cctatccttc acattgacga accaacattg
     7861 caaatgactt tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc
     7921 gctcgtaaga ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt
     7981 gaaccaacaa actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct
     8041 atcttgattg aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt
     8101 attgaaaaag aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca
     8161 ccagaagaat atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa
     8221 gatatggtcc atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt
     8281 ttgattggtt attcaactga attcctttca atgacacgtg gttacggaat catgaaccat
     8341 acttttgacc aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg
     8401 cttgtttcga tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc
     8461 ggtacagtct tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac
     8521 agccgtgata atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg
     8581 tctgctacta aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa
     8641 tctttggaat tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg
     8701 agaaaacaaa ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct
     8761 gtaaaaggat gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc
     8821 caactctttt ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg
     8881 aaagacgttt aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag
     8941 ataatgtttc acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa
     9001 gagaaaggag cgggaagatg aacgacttat taaaacaata ctttggttat gatgagttta
     9061 gacctggtca aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca
     9121 tgcctaccgg aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc
     9181 ttactgtggt tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc
     9241 aattaggtat acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac
     9301 gcttccaaga aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta
     9361 tcatgccgga ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg
     9421 aggcacattg tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga
     9481 accagctaga tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg
     9541 tgcaagtagc tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga
     9601 ctggttttga acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat
     9661 atctaatcga atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga
     9721 gaaaagaagt agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat
     9781 atcatggtgg tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc
     9841 gcttgcagct gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc
     9901 gttttgttat ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc
     9961 gggcgggaag agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc
    10021 aagtccaaaa gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat
    10081 atgaaaaatt aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca
    10141 tcctaaacta ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg
    10201 acagagaaat agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta
    10261 tgggtgagaa ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa
    10321 agctgcaagc tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc
    10381 aaaaagaaac gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca
    10441 atggtgagta tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag
    10501 aatctgttta tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata
    10561 cactatttga agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc
    10621 cgtatgtcgt attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca
    10681 gattggagat gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag
    10741 cctttttatc tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa
    10801 tacgtaaaaa tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc
    10861 aaatatgtta taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa
    10921 aaaacttgcg atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa
    10981 tcaaaacaac agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca
    11041 gatgtacggc ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa
    11101 cgagggccag ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt
    11161 ttaccaagaa atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa
    11221 gtgaaaaaaa ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta
    11281 ttgggattga tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat
    11341 ccgtccgcat cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca
    11401 ctactttata aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc
    11461 gcagttttga cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg
    11521 aaaggttggc tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt
    11581 tctatctggt acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt
    11641 cttgggactg tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt
    11701 cttcccgatt ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc
    11761 agcggagtca attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact
    11821 tttacgatct ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat
    11881 atctataatc gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat
    11941 caactagtca acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt
    12001 aacagtatcc agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc
    12061 gtagtggaag aactgggagt gattatgggg attttgatat tagcgttgct attctttatg
    12121 attgtgcgca ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc
    12181 attgggatcg gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc
    12241 gttattcctt tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg
    12301 atgctttcga tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc
    12361 ttaggtttat aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa
    12421 caggggtgaa atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt
    12481 tggaatctat gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata
    12541 tttagtagga caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc
    12601 tatcgcgaaa gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa
    12661 cttgaatttt gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca
    12721 tcatttagat atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat
    12781 tgcttcgatt ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc
    12841 gaaacaatac ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat
    12901 gcgtgtagct cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc
    12961 aaaagcagcc tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca
    13021 tatcgaagta cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga
    13081 ttgttctgtt cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa
    13141 tgaacagcaa cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta
    13201 tgtcaacgca ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt
    13261 caaccctcgc gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt
    13321 aacaacgcaa ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca
    13381 gcaagaagga attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc
    13441 gttgaataac tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt
    13501 tggcgtacgt ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga
    13561 ttcattgcta gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat
    13621 ggaacgttgc ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct
    13681 caatgtcatc acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag
    13741 tacagcaaca ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata
    13801 tatcggtgaa attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta
    13861 tgaagaacca cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa
    13921 tgtattagat gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt
    13981 gttgttgacg gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg
    14041 gacaaaggat ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc
    14101 aagtgagatg tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc
    14161 ttggcaacgg ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt
    14221 cagaggttca aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt
    14281 caaagaagca gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac
    14341 tccacaaatg gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc
    14401 tatttgctat actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta
    14461 taaagacatg gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat
    14521 ggctgggctg cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac
    14581 agatctgcca atccatctgc atactcatga taccagcggg aatggaatca tcacttattc
    14641 tgctgcttca aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa
    14701 tactagtcag cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc
    14761 agaaatcacg attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta
    14821 ttacaaaccg tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat
    14881 gccaggagga cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg
    14941 ctgggatgaa atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa
    15001 agtaactcca tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct
    15061 tacagaagaa gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac
    15121 tttcttccaa ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat
    15181 tattcttaaa ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga
    15241 cttcaacgaa gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga
    15301 agtattaagc tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca
    15361 atttggcgat gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga
    15421 aacaatcaat gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg
    15481 agaacccgat atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga
    15541 aatcactatc aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc
    15601 gacaaaccga gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa
    15661 aaaaggcgat aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga
    15721 aacggccatc gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga
    15781 cactatttcg tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg
    15841 ggaaacatga aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc
    15901 tacatggaac cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa
    15961 atatcaaacc aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact
    16021 tatatcggaa aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta
    16081 actagcggat ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt
    16141 gaagcgaaca tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct
    16201 gatatcgcgc cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc
    16261 tatactaatt tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag
    16321 gatatgaatt atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt
    16381 gatcaaacaa caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa
    16441 ctattgccat atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct
    16501 gattgggaat caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt
    16561 agaagagaag acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag
    16621 ctgctactac atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct
    16681 gattggcaaa aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat
    16741 gaattggaga agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat
    16801 ccggcgattg acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac
    16861 cgtttcatgg acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta
    16921 gtcttattgc aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct
    16981 gtttcaactg gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat
    17041 ggcttcttat cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa
    17101 agaagcgttc ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc
    17161 acctgattat cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga
    17221 tgagcatgtt gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat
    17281 tgtccaagta cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt
    17341 ttttgaaacg aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg
    17401 aatttgaaat aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa
    17461 agaatctgaa aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc
    17521 tttacttaaa tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg
    17581 ttcgaaacgt agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag
    17641 gaaacaaaac agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc
    17701 gattagctga acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg
    17761 ataacgatcg atttttgatc attgtccaag tatttttttg gtatactagt accagtacca
    17821 tttctaagga gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa
    17881 agtttagcag gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat
    17941 atgattggac catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt
    18001 ttagctatcg aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac
    18061 caagcgctaa aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc
    18121 acaataaaac ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc
    18181 gactacttgt ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa
    18241 atggaagaac tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca
    18301 gtcgaattgc cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata
    18361 acggccgtaa ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa
    18421 gttttgatcc ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg
    18481 atgaggtagt aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag
    18541 aaagaatgac attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc
    18601 aagaaaatca attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag
    18661 gcatacgaag tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc
    18721 tatatgatga attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt
    18781 ccagtatttt aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag
    18841 tgatcaacga agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa
    18901 acagtactga ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg
    18961 tattatgtag aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat
    19021 aaggaagata aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca
    19081 ggtttcttag ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa
    19141 ttgactggtg gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct
    19201 tcccaaaatg cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa
    19261 ttcaaaggcg tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca
    19321 gttggagata cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg
    19381 gattatgtaa aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt
    19441 gaagaaaaaa cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata
    19501 ggtatcacgc ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct
    19561 ggctctatcg gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc
    19621 actggtaaaa acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa
    19681 ggagaagttg gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc
    19741 gtagagattt tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct
    19801 gaaataaaaa ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg
    19861 aaaatcgtac cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga
    19921 aactaaataa ttaaagaggg gttgtgacac gagttctgtc acaacccctc tttttcgtca
    19981 aaaatcttca cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg
    20041 gacgattttg ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt
    20101 tttactggtc attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag
    20161 cttcacccaa attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg
    20221 gttttttatt atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa
    20281 tactttcttc gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag
    20341 aggatgctcg tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta
    20401 tctattcctt ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat
    20461 tagaagaagc ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc
    20521 tatatatccc tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg
    20581 aaggaaaaca gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc
    20641 catcagaact tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat
    20701 ttagagaaga aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg
    20761 gtgaaaagac agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat
    20821 cgactggcac gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag
    20881 cttggcagga cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa
    20941 acattaaaaa aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt
    21001 atgaatacgg ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat
    21061 gtacttagct atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag
    21121 cataaacaac tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga
    21181 aaaaagacta tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa
    21241 aacaaaaata tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag
    21301 caaaacgcca tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc
    21361 gaagaatttt tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga
    21421 tcttttgtca gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta
    21481 ctttttaatc aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag
    21541 catgcgatac gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg
    21601 accagtactt tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag
    21661 aagtaggagc gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag
    21721 tcagtggaga tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc
    21781 gaacgatcca tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg
    21841 aaggttcaga aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc
    21901 aagctggtgt gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag
    21961 cgttgatcga acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt
    22021 caaaacttca aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga
    22081 aatcggttga tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac
    22141 aattggggtt atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga
    22201 cgttttatcg cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta
    22261 caagagtatt catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat
    22321 acgattcgtg caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa
    22381 aagctccagc tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc
    22441 cgaggaaagg gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg
    22501 aatctttata attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc
    22561 caaattcaaa agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga
    22621 aaaagaggat cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat
    22681 attaattctt tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt
    22741 agggagagtg gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta
    22801 gggtggctgt attatttatt tcgacgtatc ggatggccac tacacgtcag tattattccg
    22861 ttgagtctac taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt
    22921 gctagtttgc tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc
    22981 atggacttat ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt
    23041 catgcgggtg gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat
    23101 ggagattggt tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg
    23161 attgtatggc atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca
    23221 ccggtattca agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg
    23281 ctgccggaaa ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc
    23341 gatttttctt cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg
    23401 tatattttcg gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg
    23461 ttttatgttg gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca
    23521 acgatcgctt ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga
    23581 aaagaaacga ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg
    23641 caaggtatga tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg
    23701 ataagtgtaa tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta
    23761 cccacgttat ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga
    23821 aatgagccat tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa
    23881 atcaaacatg gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa
    23941 aagggagagg gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc
    24001 tttttatttt taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct
    24061 aatctgaaag ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca
    24121 gattttcttt cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt
    24181 tatggtcacc cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg
    24241 cgaacggatc aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg
    24301 ctagtggaaa caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa
    24361 agtggtgagt aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc
    24421 atcttgttac ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat
    24481 tgtcaaaaaa atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga
    24541 aaatgcaagt ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca
    24601 acggttagta ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat
    24661 cgaacaaaat actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt
    24721 agtattcttt gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa
    24781 aaaaacagca attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct
    24841 gatgaatacc ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt
    24901 gaggttgacc gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta
    24961 tggacaaaat caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt
    25021 agaacataat atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt
    25081 gcgtttatac gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt
    25141 gctttctcaa ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca
    25201 ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga
    25261 agtaaaaaaa ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga
    25321 ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg
    25381 cacagggcag ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg
    25441 tcgtttgttt ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat
    25501 ttagcgattt ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga
    25561 ccttttgatt cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg
    25621 tcagcacctg aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt
    25681 tgagatgaat ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca
    25741 atgtttggca ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt
    25801 caacattcat tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt
    25861 ttccttaaca gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa
    25921 aaaatcaaaa agacgctatt gtgggaggca atagcgtcaa accaaactag atttttattt
    25981 atctatttaa acaaagtttg aatgggaatg tttggtacta agttattata taacaaagag
    26041 aaccgtttgc aaagtttttt ttgaaaaggg atacgattcc ctgttgttct ttgtttttct
    26101 attaagaaac tgcagttatt ttataaccag tatcctttga tcagtaaagg ctattctggt
    26161 gtaccgatta ttttaatgta ttcatcgata ttttttatca acattttttt tatgaccaac
    26221 tggttccgac ggttccattt gccacttact tccagcacat atttatttag gggcaaacgt
    26281 aagatatatt ttgccaaata gcgatcttta agcaggccat tgatcacacg gtcctcaagc
    26341 tgcaagttga tcccagtcag catcattggc tgttcttgta aaacgtacgt atcaattaaa
    26401 agaccttcaa aagtttccac aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc
    26461 actaattctt ccattttgat attcgttgaa gtggatgcga tcattctttc ttttgtccac
    26521 tgtaatctag tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag catcgaattc
    26581 tataggaagt ctcattggca cggcctaaat tattacaaaa cattgattaa aaaaagcccc
    26641 tgcatcgcag gagcatcccc tatatcgttt taataggtca atcaaaaagc agtatcaaac
    26701 acattttgta tcaaatcagg aaataataga taagctaagc ccagaatcaa aagtaccgct
    26761 agaagaaacc agccgattgt tcctatgaaa tactttaatt tttcgaacat tgtatcacct
    26821 cgttgtcagt atatcacgga tcagtaaatt gggggtgtta agaatctcct aaaatagaca
    26881 aaatttgttc tattttccta aagcaaatac ttgtaagata cagtaagtga taccagaagc
    26941 aatgaccggt ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac
    27001 aagtgcaaca gtgatttgtg gatcagaagc aatcaatcct acacccttgg aagataagac
    27061 agccactagc ccaccgcaaa aaacagctac ccagccaata ggggatttaa aggcttctaa
    27121 tagatcacgt agaccgattt ctcctgttgc gatagggatt agaatagccg ctgtgatgat
    27181 cgtcacccca atattgattc ctttgccttg aagccaaggg aaaaaggaac gggaggctgg
    27241 tatgagcttc ataatcaaaa caaaagctgc cgctatcatc agactttgat ttttagctaa
    27301 aattgctaca agtaaaataa ttcctaaaaa aagccaactt tccatgaaat atattcctca
    27361 ctttcttcga aacaatcata acatgttttt agaaagttac aaaagtagga attcatcctt
    27421 caagacctat tcataagctg tttttttcat aaaataaaca aaatcataaa aaaacacttt
    27481 gcttatttga cagaagacag ccgcgctttg tatagtggac aagacttaca tttttttgaa
    27541 gaatcagaaa gatcaggtaa aggtcttagt aaacgcaagc cattcagaat aaccaatatt
    27601 gtgcttcctt cgtgtcctac aactccaaga ggtagattta aaaattgcag gaagttagaa
    27661 gcaatcaaca agagaataac aagaatcgaa aaaataatat tttgtttgat gatccgccgc
    27721 atttttaaag atagccgatg actgtagaca aatttattta agtcgctttt cattaagacg
    27781 atgtctgcga tatccatagc ggcatctgtc ccttgcccca tagcgacacc taaagaagcg
    27841 gctgctaaag caggcgcatc attgactcca tcaccaacca tcgcatttac ttggtaaagt
    27901 tcattctctt ttttgactag ttgtgttttt tcttccggag tacagcttgc atagtaatgt
    27961 tcaatcccta cttgttcagc aatggccttt gctgtgccag aatggtcgcc ggttatcata
    28021 cttgtatgga tgttttggga tttaaagtag gaaattactt gttgggtatt tgcttttgga
    28081 atatctagca gacctaaaac agcaattaac tgctgatctt tagaaagata aatcacggtt
    28141 ttgccttgat tttccaaacg ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc
    28201 tcaggatcat atgcgtgctt gccaactttc cattgcgcac ctaaataggt agtttgtaat
    28261 ccaaaaccag taatttcttc tattggtaag tattggattt cttcaggtat tgtgcaatcg
    28321 aaatattgca aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat agcaacaaga
    28381 agttgttgtg ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac tggttttcct
    28441 ttagtgagcg ttcctgtttt atcaaaggca atcgctttta attcagaaag cttttctaaa
    28501 tggataccgc ctttgattaa aatcccattt tttgcagaat ttgaaagagc agctagagaa
    28561 gctggtgtag cagatgcgac aagcgcacaa ggagaagcca caactaataa taccattcct
    28621 cgataaaagc tttcatccca actccaacct aggaaaaagt gggggagaag gatcattaga
    28681 ggaacagcaa gcaaaatcaa ttttacataa ttattttcga ttcgttcgat aaatgaagct
    28741 gtttgagttg gcgtattctg cgcttcttct actaattgga taatcttagc aaagacagtt
    28801 tcggaactcg tttttgttac tcgaatcgtg atgggatttc cttgattcag cgttcctgca
    28861 tacatctcat cgcctaaata tttttctaca ggcacagatt caccgcttaa tgcagactca
    28921 tcgatcgttg cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc tcctttagcg
    28981 acaaatacca agttatccac ctttagttga gaaacatcta cttcttgcat ttgtccattt
    29041 tcttttaata attgggcttt ttgtggttga agattcatca aagcagtaat ttctttttga
    29101 cttttattag ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt
    29161 gctccttcaa agtaattacc gatcaaacag gcaccaatag cagctaaagc catcaataaa
    29221 tccacattca gtgtccgatc attccacaat tctttcaagc cttccgatgt ttgtttatat
    29281 ccaccgatga taatggcact gatataaaaa agtggggtga tggtaagaga gaatcggtcg
    29341 aagataaaac cgaagatcat aaataaaagg cagccgattg ttgctgcaaa agcttttttc
    29401 tgattagtca tcgttattag acctccttgt caaaacaagg atagcataat aaaaatgtaa
    29461 ttgataataa taacagctaa atgagaacga taatggttat catttggtga ttaagcttga
    29521 tgttgttttt attatttata atgattatta ttaaagaaaa taaaaaacca gaacatttct
    29581 caaataagag aagtgttctg gttttttcta cattgcaata aaccaataac caatcaaaat
    29641 gataccaagg ataatacgat accagccaaa agcggtaaag tcgtttttct tcaaatagct
    29701 tagtaagaat ttgatagcta agacggaaac cacaaaagat acgaatgttc ctacaagaag
    29761 aacaacagtt tcttccattt tgaatgaatt cccttcagct aaatatttga cgatcttcaa
    29821 gccgcttgcg ccaaacatga caggaatccc taagaaaaat gaaaactctg tagcgacaaa
    29881 acgcgaagca ccaatcaaga tagatcctag gatcgttgcg ccagaacggg atgttccggg
    29941 aacaagtgcc aagacttgga ataacccaac aagaagagct gcttggtacg taaagtcatt
    30001 cagagaagtc caacgaggtg ttttgttttt gttgcgtttt tcgacaataa taaaggcaat
    30061 accgtaaacg atcaaaacaa tggcaactgg taagaacttg tggaaatttg cttctaacca
    30121 atcatccaaa ggcaaaccaa tgatgatggc tggtatgcaa gcaacgatca ctttaaacca
    30181 aagtgtccac gtatcttttt tttctaccgt tgatttactt ggagaaaatg ggtttaactt
    30241 gtgaaaataa agaacgacga cagccaagat cgcccctaat tggataacga cattgaacat
    30301 cgacataaat tgtgaactgg catccagttt gatgaattca tctgctagga tcaaatgacc
    30361 agtactactg atgggcagcc attccgttat tccttcaata ataccgagaa tcactgcttt
    30421 gataatattt gcaaaaaaca taaattcctt ccttcccaat taagatacat cttatagtat
    30481 accgttattt tttgagaaac ccaatctgtt ttttaatatt aagaaaaact tgagaaataa
    30541 agcagataag tacacgaaga gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt
    30601 atgattctct ggtaaaaatt aaaatatccc cattatagaa acgagtcgcg actatgtgtt
    30661 ttcccgttat cgatataaga cgtatcattg atgccgtgga tagaaaatcg accgtttgca
    30721 taagtgacct ttgttacgct gccatttaaa agcccagcac gaacaggctg tttaggatcg
    30781 atcagattaa gcaaaaaggc gatcgtcaaa ccatgagata cgactaaaac atttccacca
    30841 ccattttttt cgcagtgatg ggcgatatct tcaaatcctg tccatacacg gtctcgcaat
    30901 gtttcataag tttccgccca cccagcggta tctaagttgt atacagcatt agcaatttgt
    30961 tcaaaagaaa cttctgtggt aaacatttca tcatatgttt taaaattcaa tacacgaggg
    31021 atcacacccc acatttccat gtcatatcca ccttcaagag acccgaaaca ccattctcta
    31081 atcctttttt caataaaata aggaatttct tttcccttag aatgttctcc tagaataata
    31141 cgggcagttt cgattgcacg accactgtca ctagaataag caaatgaaaa gtcgacattt
    31201 tttaacccgc gacctaaata atgaatgcct tcttgtcctt gctttgtgag gggtgtatca
    31261 caccagcctt gtactcgttc aattgtattg aacatcgttt ttccgtgtcg tacgatataa
    31321 agtgtagttt ctgtcatttt attctcctcc taagtcgttt taacgattga aaaaaggatt
    31381 tgtccttttt tcctgttcaa tcgtcgttgc atcgccatgt cctggataga ctgcaaattc
    31441 atcaggtaat acaaacagct gggtccgaat actatgaagt aattgttcta aattccctgt
    31501 atacagatcg gttctaccga tacttcctct aaacagggcg tcgccagaaa cgacaaaatc
    31561 atcaaaaata aagctgacgc ttcctataga atgccctggg gtagggacga cgcgaaaaga
    31621 catgtttcct aaagtataag ttttcatttc aaattcatat tctgcaggcg agacaataac
    31681 atcagcaata tcatcatgtc taccgagtcc agaaagattc aaaatcggat cacctagcca
    31741 tttttgctct agtgggctga cataaactgg gatttgataa tacgcgcgta aaggttccac
    31801 tgcaccgata tgatcgtagt gagtatgggt aagcaaaatc gctaccggct gttggtttgt
    31861 ttttttgatt tcttgtttga ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc
    31921 ttcttcgttg tagaccaaat aacagttttc ttcaatcatt cctgttttta atctttcgat
    31981 atgcatgtgc attcctcctt gatagccaaa acttctttca tccattagta taacgcattc
    32041 attgctagga aacaaaaaca ggtacttatt tctctttact tatctgaatt atcagactat
    32101 atgaaaagta tttggatatt ctaaagagaa ggaaagtaag ttttcatagc attttaatat
    32161 gtccttgtta cattagattc atccaaaaat ccaaattcta tatcctatat ccctgcctaa
    32221 tcagcagggt gtttttttat gtttggaaag agagctcttt ttgctaaact aggttttttc
    32281 aggtaatctt agagagaaag aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg
    32341 gcacttagta gaatttattt tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca
    32401 tactgcatta gaaggagcga gaatccaccg caagctccaa aaagatgcgg gagaaaacta
    32461 tgaaaaagaa gtctttttaa aaacaacagt tcaattagat gaatattacc tgatcgttga
    32521 aggccgtgca gacggtattt tcaaaagaga cggtatttat tatatagatg aaatcaaaac
    32581 ctccgaacca cgatttgaag atttagaacc agaacaagtg gaacttttct ttcatcaagc
    32641 aagggtgtat gcctatatct atagtcaaga acatgattta caagaaataa atctgcaatt
    32701 gacttacttc cagacggcag aagaagtgat cacgagaaag gttgaacatc agacaagaga
    32761 gcagttagaa acttttttta aaaagctgac tgaagattat aaagaatggc ttgtttttca
    32821 agaaaactgg cgcactgtac gcaatgcctc tttgatggct ttgaaattcc cccatgatga
    32881 gtataggaaa ggtcagcgtg aactagcggt agctgcttat aaaacgatcc gtacgaaaca
    32941 gaaattgttt gtagaagcac ctaccggaac aggaaaaaca atctctacgt tattccccgc
    33001 tttgaaagct gtaggggaag aagagggaga aaagatcttt tatctaacgg ctaaaacaat
    33061 aactagacaa gtagctgaag atgctatgac agcattaaaa gatacaggag ctgaagtaaa
    33121 aagtgtcact ttgacggcga aagacaagat ctgcttttta gatgagacga cctgtaatcc
    33181 agatcagtgc ccatatgcca atggttatta caatcggatt aatgaaggtt tatgggatct
    33241 tttgaatcat gagaatcaga tcactcgtga agtcatcgaa acttatgcaa gaaagcatac
    33301 actttgtcct ttcgaactat cattagatgt cagtatctgg tgtgacgtaa tcattgggga
    33361 ttacaattat ttatttgatc caactgttta tttaagacgt ttttttgaag atgaaaaaaa
    33421 cgaggactat ctgtttttga ttgacgaagc acataatttg gtgaatcgtt cccgagagat
    33481 gtattcggca gaactatctt atgaaaaaac gaaacgaagt aaggaagcta taccaaagga
    33541 attcaaaaaa cttcatcgcc gtttcaacaa actattaaaa gaatttgatt cgatccgaga
    33601 aatagcaaaa gaagatcatt gggattatca tcatcaaaaa gcaccggctg aatctttagt
    33661 aaaggcaggt tatcaattaa gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca
    33721 cccatcacaa gaacaattgc ttccttatta ttttgatttg cttcattttt tgaaggtcag
    33781 tgaattttat gatgatcact acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt
    33841 aaaagagttt tgtattgatc caagcttgtt tcttgagcag tcgttggata aaggaaaaag
    33901 cagtattctt ttttctgcta gtttttcacc actctcttat tatcaggaaa cactgggggg
    33961 acaaaaaagt ctggcttaca aactgccgag tcctttccca gaaggaaacc agcaagtttt
    34021 catcgcaaat tacctagaga cgacttatcg aaaaagggaa cacagtctgg caaaattagt
    34081 agagacgatt catgcttttg ctgatggaaa ggtcgggaat tatttcgttt tctttccttc
    34141 ttatcaatat ttagatcttg cggtggaagc attcagacag caatatcccg acagcaatgt
    34201 gctaatccaa gaaacggaca tgaatgagga agaacgcgaa cgatttttaa caaaatttca
    34261 aacaaatccc gctgaaacat tgattggatt ttgcgtactt ggcgggattt tttcagaagg
    34321 aatcgatttg agaggggacc gattgatcgg cagtatgatc gtaggtgtag gtttgccgca
    34381 gatgaatcat gagcaggaac tgatcaaatc atattatgat gagaaggaac accttggatt
    34441 tgcttacgct tatcagcttc ctggaatgaa caaagtcttg caagctggtg gtcgagtgat
    34501 tcgtggcatg gcggatcaag gaatcgttgt attggcagat caacgatttt ctagtttttc
    34561 ttatcgacgt ttattcccac aacattggca aacagcaaga gaagtacgaa gtgttgggga
    34621 attggaagaa ggaatacagc gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga
    34681 ggaatgacag acgtcagcct cgttttttga ttaacgatag ttagtgaatt gcaactcgat
    34741 tggtaagtca agtttacgta ataaagcaat cacagcttgt agatcatcac ggttcttacc
    34801 tgtcactcta atctgatctt cttggatttg tgcttttact ttcagttttt catttttgat
    34861 agctgtagtg attttcttcg cgttttcacg atcgattccg ttgaccaaat cggcagtttg
    34921 tcgagctttg ccacctagag cgtgttctga ttcggaaaaa tggatatttt tgattggcac
    34981 atttcttttg atcagcttac caaataatac atctttgatc tgttcgattt tgaaatcatc
    35041 atcgctcaat acaactagat tgctgttctc taatttgatt tctactgtag aatccttgaa
    35101 gtcaaagcga ttggacaatt cttttgttgt ttgctgaatc gcatttttta cttcttctgt
    35161 attgacttct gatacaatgt cgaagctagc atcttttgcc ataggaaccc tcctgatata
    35221 catataaata tttaccatac atctatctta tcataggatt agggcagatt cttacctgtt
    35281 tgctaaaatt cttctcattt gagttcgaag tattttattt taatagataa actgttattc
    35341 ttacatttgg aataccaaag taggggagag gtgcttgtga gtaaaattat gactgcgtat
    35401 cataaaaatg aaagattaaa gaaaatagtg gttattttga taaccggatt gaccgctgcg
    35461 gtcgctttaa attatttttt gattccagcc aatgtctttt ctgcagggat gaatgggatc
    35521 gctcaaatca tttcctcgct tttatatgaa tggtttcata tcaagataga cacaggaaca
    35581 tttatttttc ttttgaatat ccctgtgttt gtattagggt ttctcaaagt aggaaaacga
    35641 gcaacgatcc ttagttttgt gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca
    35701 gtcggacaga tcactgataa tattttgatg aatgccctag taggcggtgt tttattaggt
    35761 cttggcgtag gattatcttt aaaaatgggc tttacaactg gtgggatgga tatcatctct
    35821 cttgtacttt cacaaacaac tggaaaaaca gtcggaaact acatgctgat tctaaatggt
    35881 atcattgtag tcatcgcagg cttcttcttt aactgggaaa gtgccttgta tacgattatt
    35941 tccatttatg caatgactca agtaattgat gccatccaca cgagtcatca aaaagtaaca
    36001 gcaatgatcg ttaccgtcaa gccagaagag gtaacagaag aactagcaaa acggatggta
    36061 cgtggaatga cattactgcc gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc
    36121 atgatggtca tcacccgcta cgaaatgtat gatcttgatc agatcgttca tgaaatcgac
    36181 gacgatgcat ttatcaatat tttaccaact cattcagtat ttggccggtt tgcaaatgaa
    36241 aacgaacaaa aaatgtatcg aacaactggc gtattgccag aaataaaaaa tcgttcacaa
    36301 aaaaaatgag gaatcaggtt tattttagaa taaatttaat ctattacatc taataagaac
    36361 atttttataa atttaaacga gaatcttact tctaaattct catttacaag cagctaaaaa
    36421 aagagtatga taaataaaaa tcgtgttaag gagtgtcgta cgatgagcga agcaaaccaa
    36481 gaacggtctg gacaagaaat agaactaatc aaagaacgta ttcttgctgc cttggaaatg
    36541 gtgattgatc ctgaattagg aatcgacatc gtcaatttag gcttgattta tgatatagaa
    36601 ttcaatcctg aaaatggaga aacagtcatt aagatgactc taacaactat gggctgtcca
    36661 ttagcagata ttttaacaga atctatccat gacgcattaa aagaagtgcc agaagtaacg
    36721 aaagcagaag tcaaactagt ctggtatcca gcatggacga cagataagat gagccgttat
    36781 gcacggattg cgttggggat acgatagaaa tcttggctga ctagctatga gaagcaaata
    36841 ggaaaaacaa aaaagccttt tatcctttct agaagaaaag agataaaagg tttctttgtt
    36901 tataaaattt tgtctgctat attataagtt aattaaataa atgtgttcta ttgataaagg
    36961 acattcaaat ccctttcctt ttggaatcct tcttttttac atcagtttga ttttcgtata
    37021 gtattctttc tcatcttcac gataaatcgc agccccattt ttttgttgtg ctttaagact
    37081 gccaagattt tttttattta cagacaaaag tgcctcatca atgccgattt tttttgcctc
    37141 ttgtagtact aatgctaatc cttgtgtggc atatcctttt ttacgataat taggggagat
    37201 gccgtaacca atatggccag cgccgtttct taatgtttcg ttcagtttat ggcgcaaatt
    37261 aaaaattcct acatactcat gttcatcact ttctaaaata aagacggttg cgggaacgta
    37321 actctcagga agatcgattc ctaaagacgt tttttcgcat ttttttacat aatctaaaaa
    37381 ttcagcaaat gtatagccat aagcattgtt gataaatcca ctctcatttg gtccaaagct
    37441 agtttgtagc tgataagctt tctttaaatc tgcttcccat agtttgatca gtttcatcgt
    37501 attcacctct ttaagtaacc ttattcattt gttttctact atagcactat tgttgtaaat
    37561 agccatgcct atttttattt attgtgtcat atcgtattaa gaatcatcga acaatacaaa
    37621 tatgaattac aataaaaaaa ttatttgaag ttcgttcatt cgttacttat gatctctaga
    37681 agaaagaaaa cgtgaattac ttcagcgtct agaattccag aaggatagta atcaacagaa
    37741 gaaataatca atatccaaat gaaagaagat agctgaatcg agaaaaagag gaaaaaagaa
    37801 atgaagaaaa aaataaaata gttactaatc ccagagtcgt tcgtttaaca ttgaaatcga
    37861 tcatatggat aaaacagatt ttttcaccaa ccctcaatag atgatggagt tgctcggatg
    37921 aattatttgt gtagatggca taagggaggg cgtacagaaa tgaaaaagaa caaaaatatg
    37981 ttcttgacaa aaaacacatg taatcctaaa ctaaaagatg tgatgcggct atggcggaat
    38041 tggcagacgc gctagcttca ggcgctagtg agggtgacct cgtggaagtt cgagtcttct
    38101 tagccgcata atttgttttt ttataaagag aaggtacacc tggagcgatg agaaagaaaa
    38161 taacatatta tctaaaagaa tataggaaaa attttaaaca acggaaccag attcaaataa
    38221 atcaaaaaaa atttgtgtga aggtgggaca gtttaaaacc taatagattg attcaaatag
    38281 tagatttata gattctgata caagaaatac tggcaaacga tagttaagga ataaagacta
    38341 aacttaaact caaaaagagg tcatgcgaaa gaataaatcg catgacctct tactatattt
    38401 tgaatgtgtt taaacgttca aataatgaat cttagtacca accgtttgct tcccagaatg
    38461 ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt
    38521 ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa cggccgatgt
    38581 aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg
    38641 cagagcttgt acttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt
    38701 cagcaacagg cgctgcttgt gtttccgttt gaaccgtttc ttgtacaaca ggttgttcag
    38761 cttgaacagg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt tcagcagctg
    38821 cttgtgcaga gccttctgtt gggattgtta attgttgtcc tgagta
//
LOCUS       DACCIQ010000018        38192 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCIQ010000018 DACCIQ010000000
VERSION     DACCIQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.018"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            113..760
                     /locus_tag="IVM62_000952"
     CDS             113..760
                     /locus_tag="IVM62_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP6844925.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            775..2301
                     /locus_tag="IVM62_000953"
     CDS             775..2301
                     /locus_tag="IVM62_000953"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP6844926.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2350..5148)
                     /gene="ileS"
                     /locus_tag="IVM62_000954"
     CDS             complement(2350..5148)
                     /gene="ileS"
                     /locus_tag="IVM62_000954"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP6844927.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5410..6117)
                     /locus_tag="IVM62_000955"
     CDS             complement(5410..6117)
                     /locus_tag="IVM62_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP6844928.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6138..6920)
                     /locus_tag="IVM62_000956"
     CDS             complement(6138..6920)
                     /locus_tag="IVM62_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP6844929.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(6969..7256)
                     /locus_tag="IVM62_000957"
     CDS             complement(6969..7256)
                     /locus_tag="IVM62_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP6844930.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7271..7870)
                     /locus_tag="IVM62_000958"
     CDS             complement(7271..7870)
                     /locus_tag="IVM62_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP6844931.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7884..8561)
                     /locus_tag="IVM62_000959"
     CDS             complement(7884..8561)
                     /locus_tag="IVM62_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP6844932.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8578..9819)
                     /gene="ftsZ"
                     /locus_tag="IVM62_000960"
     CDS             complement(8578..9819)
                     /gene="ftsZ"
                     /locus_tag="IVM62_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP6844933.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9842..11167)
                     /gene="ftsA"
                     /locus_tag="IVM62_000961"
     CDS             complement(9842..11167)
                     /gene="ftsA"
                     /locus_tag="IVM62_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP6844934.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11323..12543)
                     /locus_tag="IVM62_000962"
     CDS             complement(11323..12543)
                     /locus_tag="IVM62_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP6844935.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12558..13646)
                     /gene="murG"
                     /locus_tag="IVM62_000963"
     CDS             complement(12558..13646)
                     /gene="murG"
                     /locus_tag="IVM62_000963"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6844936.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13672..15033)
                     /locus_tag="IVM62_000964"
     CDS             complement(13672..15033)
                     /locus_tag="IVM62_000964"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP6844937.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15047..16009)
                     /locus_tag="IVM62_000965"
     CDS             complement(15047..16009)
                     /locus_tag="IVM62_000965"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP6844938.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16035..18227)
                     /locus_tag="IVM62_000966"
     CDS             complement(16035..18227)
                     /locus_tag="IVM62_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6844939.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18228..18629)
                     /gene="ftsL"
                     /locus_tag="IVM62_000967"
     CDS             complement(18228..18629)
                     /gene="ftsL"
                     /locus_tag="IVM62_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP6844940.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18634..19593)
                     /gene="rsmH"
                     /locus_tag="IVM62_000968"
     CDS             complement(18634..19593)
                     /gene="rsmH"
                     /locus_tag="IVM62_000968"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP6844941.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19614..20045)
                     /gene="mraZ"
                     /locus_tag="IVM62_000969"
     CDS             complement(19614..20045)
                     /gene="mraZ"
                     /locus_tag="IVM62_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP6844942.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20215..20583)
                     /locus_tag="IVM62_000970"
     CDS             complement(20215..20583)
                     /locus_tag="IVM62_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP6844943.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20754..21710
                     /locus_tag="IVM62_000971"
     CDS             20754..21710
                     /locus_tag="IVM62_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6844944.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21783..21899
                     /locus_tag="IVM62_000972"
     CDS             21783..21899
                     /locus_tag="IVM62_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP6844945.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21954..23636)
                     /gene="recN"
                     /locus_tag="IVM62_000973"
     CDS             complement(21954..23636)
                     /gene="recN"
                     /locus_tag="IVM62_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP6844946.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23654..24103)
                     /locus_tag="IVM62_000974"
     CDS             complement(23654..24103)
                     /locus_tag="IVM62_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6844947.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24210..25028)
                     /locus_tag="IVM62_000975"
     CDS             complement(24210..25028)
                     /locus_tag="IVM62_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP6844948.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25037..25927)
                     /locus_tag="IVM62_000976"
     CDS             complement(25037..25927)
                     /locus_tag="IVM62_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6844949.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25929..26153)
                     /locus_tag="IVM62_000977"
     CDS             complement(25929..26153)
                     /locus_tag="IVM62_000977"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP6844950.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26158..27495)
                     /locus_tag="IVM62_000978"
     CDS             complement(26158..27495)
                     /locus_tag="IVM62_000978"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP6844951.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27496..28350)
                     /locus_tag="IVM62_000979"
     CDS             complement(27496..28350)
                     /locus_tag="IVM62_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP6844952.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28447..28896)
                     /gene="nusB"
                     /locus_tag="IVM62_000980"
     CDS             complement(28447..28896)
                     /gene="nusB"
                     /locus_tag="IVM62_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP6844953.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28889..29314)
                     /locus_tag="IVM62_000981"
     CDS             complement(28889..29314)
                     /locus_tag="IVM62_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6844954.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29560..30624)
                     /locus_tag="IVM62_000982"
     CDS             complement(29560..30624)
                     /locus_tag="IVM62_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6844955.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30796..31041)
                     /locus_tag="IVM62_000983"
     CDS             complement(30796..31041)
                     /locus_tag="IVM62_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6844956.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31180..31554)
                     /locus_tag="IVM62_000984"
     CDS             complement(31180..31554)
                     /locus_tag="IVM62_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP6844957.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31637..32551)
                     /locus_tag="IVM62_000985"
     CDS             complement(31637..32551)
                     /locus_tag="IVM62_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP6844958.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32572..33375)
                     /locus_tag="IVM62_000986"
     CDS             complement(32572..33375)
                     /locus_tag="IVM62_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP6844959.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33408..34397)
                     /gene="manX"
                     /locus_tag="IVM62_000987"
     CDS             complement(33408..34397)
                     /gene="manX"
                     /locus_tag="IVM62_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP6844960.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34500..34991)
                     /locus_tag="IVM62_000988"
     CDS             complement(34500..34991)
                     /locus_tag="IVM62_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP6844961.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35182..38016)
                     /locus_tag="IVM62_000989"
     CDS             complement(35182..38016)
                     /locus_tag="IVM62_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6844962.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
BASE COUNT        10646 a         8310 c         6477 g        12759 t
ORIGIN      
        1 gttgtaaaaa cgtctaaaaa tcctttgttt tttatttaga aaaatagtca attaacgttt
       61 tttttgttac acttactaat gatggttttt taccatgaaa ggatgatcga atatgacacc
      121 aaatcgagaa gattatttga aaatcatttt agaactaggt ggagatacaa cgaaagtcaa
      181 caataaacaa atcgtatcta gtctagatgt ctcagctgca tctgtcagtg aaatgatttc
      241 taaacttgtg aaagaagagc ttgtagaaca ttccccttat caaggtgtac aattgactgc
      301 aagcggactg caaaaagcta gcagtctgat tcgcaaacat cgcttatggg aagttttttt
      361 agttgaacat ttagactata gctggaatga agtccatgat gatgctgaag tattggaaca
      421 tgtgacttcg gaacatcttg cagatcattt agaaaactat ttgaatcatc ctgaacattg
      481 tcctcatgga ggatcgattc ctaaaaaaga ggaagttgtc catgaagaac gccgacaaac
      541 attggagtcc tatcctgtcg gtacaaaagt gcgtatcgca cgagtgcttg atgaaaaaga
      601 attgttggat tacttagtca ctattgacct gaatatcaac gaagaatatg agatcgttga
      661 catcgcagct tatgaaggac cgatcactat ccaaaatgat agaaagaaaa tccccgtcag
      721 ctttaaggcg gccagcacga tttttgtcga taaaatataa aggagtgtat ccacatgtct
      781 aatgaaaaaa atgtattgtt tacgatattc ggcggtacag gtgacctagc ccagcgtaaa
      841 ttgtacccgt ctttgttccg cctttacaga aaaggaaatt taggcgagca ttttgctgta
      901 atcggaaccg ctcgccgtcc ctggagcgat gagcactacc gtgaagttgt caaagaaaca
      961 atccgtgcat tgaatccttc agatgaagaa gcaactacct ttgcaagcca tttttactat
     1021 caatcccatg atgtaaatga ttcaacgcat tatcagacat taaaagaatt gtctgaccga
     1081 ttagatggac actatcaatt aaacggtaac cgtgtctatt atttggctat ggcacctcaa
     1141 ttttttggaa cgatcgtttc tcatttgaaa tcacaaaata ttatgacaaa aaatggcttc
     1201 aatcgtttga tcatagaaaa accgttcggt tctgattacc agtctgcttt tgaattgaac
     1261 cagcaaatca gagaagtatt cccagaacaa gatattttca ggattgatca ttatttagga
     1321 aaagaaatga tccaaaacat ctcagctatc cgttttgcca ataatatttt tgaggctcaa
     1381 tggaacaatc gctatatcga caatatccaa attacttttg gcgaaaacct cggtgtagaa
     1441 gatcgcgggg gctactacga tcatagtggc gcacttaaag acatggtgca aaatcatatt
     1501 ttacaggttg tagcattact tgcaatggaa ccgccagtag cattttccga aaaagaaatc
     1561 cgtacagaaa agatcaaagc attgaaagct atccgcattt ataacgaaga agaagtgtta
     1621 gaaaacttcg ttcgcgggca atatgctcaa ggtgaattgg atggtgtaca attcaagggc
     1681 tatcgcgaag aagacaaagt ggatgctcaa tcagagactg aaacattcgt cgcaggcaaa
     1741 ttcactatcg ataacttccg ttggtcaggt gttcctttct atgtacgtac aggcaaacgt
     1801 ttgactgaaa aagggactcg gatcaacatc gtattcaaac aagttcctgt taatgtattc
     1861 aaaacatctg tcgatgaacc atgtgacgat acgacacttc cacctaatgt tctaacgatc
     1921 tatattcagc caactgaagg attttcttta tccttgaatg ggaaagaagt agggcaagga
     1981 tttgaaacgg aaccaatcaa attggaattt agaaatagtg cagaaatggt tgaaaatagc
     2041 ccagaagctt acgaaaaatt actattagat gcgttgaatg gtgatggaac gaacttctct
     2101 cattgggaag aagtcgctca atcttggcat atcgtggatg tcattcgaaa agcttgggac
     2161 caaacacaac cggattttcc caactacaaa gctggatcaa tgggacctaa agcagctttc
     2221 gaactacttg aaaaagacgg attcgaatgg atctggcaac cagataactg gtatcgtgaa
     2281 cgtggaaaat taaacgattg ataacaaaag ggggtgtgac aaagaacatg tcacaccccc
     2341 ttttcccttt tacttttctt cgaatccttc agctacagct tcaggataga agtcttcaac
     2401 gatttttgca caacgaccgc atagatgagg aagtttttca tctactccta catctttacg
     2461 aacttggcga cagcgatcgc aagtttcccc gtctgctttt tccactgtaa tagcaacatc
     2521 atcaaatacc atagcgttat ctggcacttg ttcatctgct gccataactt caaagtagtc
     2581 tggtgaaacg atcaataatt gtgcaatatc tgcatccaaa gcttttaaca tttcacgaac
     2641 ttgttgtttt ggataaacag ttagttttgc ttccaaagat ttaccaatca atttgctgtt
     2701 tcttgcttct tccaatgctt ttaagacatt atcacggaag tccatgaaag cagtccaggt
     2761 atccatcaat tcttcttggt ttgcaaactc ttcataacca ggaaactcgc ttaactgaac
     2821 gtactcctct tcttctttca ggaatgacca gatttcttct gctgtatgcg gaatgatagg
     2881 agtcaataat tttgtcaaag ctacagctgt ttgatagaaa acagtttgca tgcaacgacg
     2941 ttgataatca tcttctgctt caatataaac tacatcttta gcaaaatcca ggtagaatga
     3001 cgatagatca accgtcaaga agttcataac tgtacggtaa atatgcatga agttatattt
     3061 gtcgtaccct tcttctcgga tttctttgat tacttggttc aatcgaacgg tcatatattt
     3121 gtctacagaa cgtaaatcat tgtacgaaac agtatctttt ttaggatcga aatcttctgt
     3181 attcgctaat aagaaacgca tcgtatttct gattttacga tatacttctg ccacttgact
     3241 taagatatcc atagaaacac gaacatctgc ttcataatct acactagata cccataagcg
     3301 aaggatatct gcaccaaatt gtttgatcac tttttctggt gcaatgatgt ttcctaaaga
     3361 tttactcatc ttacggcctt caccatctaa agcaaatcct tgagaaataa ctgatttgta
     3421 tggtgctaca ccattgatag ccacactagt cgtgatactt gagttgaacc agccacgata
     3481 ttggtctgat ccttctaaat acatatctgc tgggaatgat aattctggtc gctgacgtaa
     3541 aacagcttca tgagaagaac cagaatcaaa ccagacatcc atgatatctt tttcttttgt
     3601 aaattcgcca ttaggagaac ctgggtgggt aaatccttca ggaagaagtt ctttcgcttc
     3661 tttttcaaac cagataattg atccatgttc agcaaataat tttgccacat gttcgattgt
     3721 ttctggtgtg atgattgctt ctccattttc agcatagaaa atcggaagtg gtactcccca
     3781 tgcacgctga cgggaaatta cccaatcgcc gcggtcacgg atcatattat acaaacgtgt
     3841 tttaccccat gggataaccc actctacttt ttctacttca tccaaaatat tctgacggaa
     3901 tttatcgata gatgcaaacc attgtggagt tgcccgatag atcacaggct ttttcgtccg
     3961 ccaatcgtga ggataactat gagtaaagaa atctagcttc agcaaagctc ctttttcttt
     4021 taatagatca gtgatcatag gatttgcttt gtcataaaag ataccttcaa atccaggtgc
     4081 ttcttctgta aacaatcctt ttgagtctac aggtgacact acagatagtt tatatttgct
     4141 accagcgata tagtcatctt ccccatgtcc tggagcagta tggactaatc cagtaccagc
     4201 gtctaaagta acatgatcgc ctaaaatcac aagtgattca cgttcataga atggatgact
     4261 tgcagtcata tattccattt cttgtccagc aaattctttt agaactgtta cattttccca
     4321 accgatttct gattggactg tttctagcaa atctttcgct acgacaaact tacggccatc
     4381 ggcttccacc aatacatacg taaattttgg atttacagca ataccttggt ttgctggcaa
     4441 tgtccatggt gttgtcgtcc aaatgacaaa agctgtatct tcatcaagaa tatctttgcc
     4501 gtctttgaca ttgaaagcta caaaaataga tggagatttt acatctttat attcaatttc
     4561 agcttccgct aaagaagact cacttgacgg agaccagtaa attggtttca atcctttgta
     4621 aatatagcct ttttcagcca ttttaccaaa tacgcgtact tgtgcagctt cataagaagg
     4681 atctaatgta acataaggat gttcccaatc tcctgcaact cctaggcgtt tgaaatctgc
     4741 tcgttgctta tctacttggg aaagtgcata ttcacgacat ttttccaagt attctgccat
     4801 ggacatttct ttacgtttca cgcctttatt agccaatact tgttcaatcg gcaatccatg
     4861 tgtatcccat cctggaacat atggtgcacg atagccagac attgattttg aacgaatgat
     4921 aatgtcttta ctgattttat tcaatgaatg tcctaaatgg atattaccat tggcatatgg
     4981 aggtccatca tgaagaacaa agctaggttt tccttcgttc aactcttgtc ttttttggta
     5041 gattcgattt tcttcccatt ctttttgcca ctcgccttct cggtttggca aattaccacg
     5101 catagggaat tttgtttttc ctaattgcaa ggtttctttc attttcattc aaccatacac
     5161 tcctttattt gttttccgga cacaaaaaag catgttcagc cgcaagggac gaacatgccg
     5221 tggtaccacc caaatttagt tgcatctatc tagtgcaaac tctctgtgac caataacgag
     5281 gtcagccgct gcttcttact ctaatttcgg aaaagcaggt ctcaagtaga ggatctttca
     5341 ccatgcagtg tcttaccgaa cttccactct catcggctcg ctttaagaat aacaaagtgc
     5401 ttttctgttc tactcgtttt cattttcacc aggcacgatc gagatatcaa tagcctgtgt
     5461 atcaaatgcc gtcataggtg cttctatgac tcctgtgtct gagtttacat cagtatcatt
     5521 ttcattgtca agctgttttg ctaaaacttc tttgatcact tcatggctgt cagtcacgta
     5581 gctggagaat ggttgtaaaa tttcatccca ttccggactt ttcacttgtt ctaattgaga
     5641 ctcaagcatc aagctgatac gttgatggaa tactcgtgtt tttttcttaa gatcttcagt
     5701 ttctgtcgca agctgtcttg ctttttctgt tgcatctgtc aagatttttc ttgctttttc
     5761 ttctgcatca gttgttaatt gatgtgcacg tttttcagcg ttcgcaacta actcatctgc
     5821 tttgttttgt gcagaagtaa cgatcacttc ggattccttg cttgcacttg tttttacttt
     5881 atcagccgta tcttgagcta cgatgattga ttggttcaaa gcatctttta attcattgaa
     5941 gtactcaagt ttttcttcag aatgtttgac tgctttttct aattctcgat ttctttgagt
     6001 caattcctca tagtcacgaa caactaaatc taagaaatca tctacttcgt cttgattata
     6061 tccccgcatt tttgtcggga atgtcttatt ttggatatct aatggagtta aagccatgag
     6121 taaaccacct ttttatttta tttacgtaat acaccgaaca gtaccctgta cttttctttt
     6181 ttcgtcttcc cctcaagttc ttggatctgg actctgccat aacctctgac tgaaataata
     6241 tctaatagtt caagtacaaa atcaggtctt gtattttctg tccagttcac ttttacttta
     6301 ccagcttcga tcagctgctt ggaccgctgt ctggaaatat tataaatctc agagatcaca
     6361 ctgtctagtc gcatggaact aactgttcct ctttcttgtg tccagctatc tttcggcata
     6421 atcatttctg tgtatttgat tggttccaag cgtacagcaa cctttccaat ctttgttact
     6481 tgtgcagcaa caaaatgatc cacctcattt gcaataaata cttgccagcg atcaccgtcc
     6541 gagatgatat cgccaaagta agagcgtttg atccccgttc ccactaaagt tccaaggatt
     6601 ttcccatgac ttaggctggc aaatttgcta ggataatgga tttcatacaa tgctgtttca
     6661 aaatcttcct ctacaggttc gtaataatcg gggaaaatca aacatctttt tctttctgcc
     6721 tgttcatatc cgccataaaa catgaaacgc aaatcactgc tttgtcgaat cagtgtttct
     6781 aaaatatagg attgtcttgg gtcaagaaat tcggtcagat agggtgcata ttgtccttga
     6841 acctgttcaa tccaatcgcc taccgaatcg ataaatgtac gttcatctgc ccgaaagtgc
     6901 tggtatacat ttgcatccaa aattttcccc tcctatctat tctgagaata aagattttgt
     6961 tgcttcaatc aataaactaa atggacagtt atccatataa cgatctgtgc taaaccttgt
     7021 acggctaagt tcaagatcag gattgcccct aaaattgtga aatccaaggg tccaatcctc
     7081 aaaggcagac gatcgaacaa actgatatac ggttcgcaga tcttagctaa taatcgtcct
     7141 agccctgatt gatacccgcc tggaaaccaa gagagcaatg catataccat caagataatg
     7201 ctatagaaat aggctgcccg ttgaagtaaa tgaagtaata gtgcaatagt ataaatcgta
     7261 gttcctcctc ttacagatca aacaaattcg catctgcaaa agattgagcg gttgaactgt
     7321 caatctccat cccagtcggt gtacaaagaa aaatctcatc tccaacacgt ttgatatctc
     7381 catctagtgc ataaactgtt cctgttagaa aatcaacgat tcttctcgct tgtttttctt
     7441 ctactaaatg aaaattcact aaggttgctt ctccagcaat gattcgttta gcaatcgcca
     7501 tagcttcaga ataagctctt ggttccatta cagtgatttt tccagcaaga tttgctttag
     7561 gttgtgtaga ggttcttgaa gctgttcttt ctttcgctac agtttttgta ggtgattgat
     7621 gtagttcaat tacttttttc ttttcagccg gctgaatact ttcatgcgtt acaggacgtg
     7681 aagtcgccgt tttttgtttt tcatgagtat tcgcagcaga tacagaatga gttgattgat
     7741 tctgtgttgt atttttttta gttggctgta cagccttttt tgttgtttca ggataatctt
     7801 ggtattcgta ctcttcctca tcagctaatc cgaaaaaatt agcaattgct cctctgttta
     7861 aaagcggcat ttttgttcct cctttattcg tctttgaata gcgctgttcc tacacggata
     7921 aatgttgcac cttcttcaat cgcaatttga aagtcttgac tcatccccat acttaattca
     7981 ctacatggtg catgggcata accttttgct tgaacaagat cacgcaattc tttcagcgta
     8041 ccaaacactt tatggatttg ctcatcagaa gcttctttag gcgccatagt catcaaccca
     8101 acaaccttga tttttgtgca ggcagctaaa gattccacaa acggtaaaag ctcatctggt
     8161 gaaattccat gttttgattc ctcaccagaa acatttactt caataaaaca ggataattct
     8221 ttctctaacc gcttctggat ttcttctgct aatcttaaac tgtccaacgc atgaaagtaa
     8281 tcaatttcat ttacaatcaa ttttaccttt ctgcgctgta aattaccaat taaatgccac
     8341 tttatttcag gaaaatcaaa taaatcttgt ttcttttcta ataatttatc tactcggttt
     8401 tctgcacaat tcataacgcc agctctaacc aattcttcag cttgagcgga ttcaacagat
     8461 tttgttacag ctactaatgt aacttctttc ggatcacgat gaacaagagc acacgaatcc
     8521 tgaatttctt gcttgatttt ctgcaagttg tcagcaatca tgtgctcttc ctctccttta
     8581 ttttttacga cggaaaaacg gtggcgtatt taattcatca tcgccatgag attttgtttc
     8641 ttcgcggttg aatgtatcaa attctttttt ttcgatattg tcaaaattgg aatcatcaac
     8701 tctaggacga acattttgtt cacgtctgat atcccaatcg ccaaaactgt tttcttcttc
     8761 tgaagaagtt ggtttagctt gatccatatc taatacagtc ttttgagcag gtgattgcat
     8821 ttgtgcttgt ctagctggtc ggctagactt gcgttccttt tttgattcat cgatacctgt
     8881 tgcgatgacc gtcacacgga tttcatcgcc catttcttca ttgatagatg tacctagaat
     8941 gatatttaca tcgccagttg ctgcatttgc tacgatatct gaagcatctt gtgcttcgaa
     9001 caatgtcata tccaaaccgc ctgtgatgtt aagaagtact tgttcagctc catcaataga
     9061 tgtttcaagc aatggagagg agattgcttt tttcgttgct tctactacgc gatcttctcc
     9121 actagccaca ccgatcccca tcaatgctgt accttggttt tccatcactg ttttcacgtc
     9181 agcaaagtca aggttaacgt aacctggcgc tgtgatcaaa tcagagatac cttgtacacc
     9241 ttgtcgtaaa acattatctg cttcacggaa agcttcaagc atcggtgtct ttttatctac
     9301 aacttctaat aaacggttat ttgagataat taataatgta tccacatttt ctttcaaacg
     9361 agcgatacct tctgcagcaa aacggccacg tttcggtcct tcaaaagtga atggacgtgt
     9421 cacgacacca acagtcaatg cgcccaattc tttcgcgata cctgcaacga ttggtgcagc
     9481 acctgtaccg gttcctccac ccattcctgc agtgatgaaa atcatatctg caccgtctaa
     9541 agcttcgcgt aaggattgtt cactttcttc tgccgctttt tgtccaactt ctggctgaga
     9601 accagcacct aaaccacgag tatatttggg gcctaattgg ataactgttt cagcttttga
     9661 attttttaat gcttgcacgt ctgtgttggc tgtaatgaat tcaacacctt taacgttttc
     9721 ttcaatcatt cggtttacag catttccacc gccaccaccg actccgatga ctttgattac
     9781 tgcgccgtca ttgatattat tatcaattga gaattccata tcttattcct cccaggatga
     9841 attaatcaaa aatatttgta aagaaaccct tgattttatt ttttacattt tctccggact
     9901 cttttggttc cggctcatcg tagattgttt cttcaggtgc ttcatagctc tcatatgtgt
     9961 ttgttgtttc ttgttcaact accatttgac gagctgctga ggtttcaccc gtaacagcaa
    10021 tttttgccaa ttgatacact tcactcaagt ttgctgaata atccacgata cttattacat
    10081 ttgtaaatac cgggtttctc agccccatgt gatttggaac atacaatttt acatttacgc
    10141 cgaagatttc ttgtgccaag tccacaacac caggaagact agcagctccg cctgtcaata
    10201 cgacgccacc tggcaactct aatgcttcga tttggtccaa tgcatctttt gctttgttga
    10261 aaatctgttc cattcgagct gaaataattt ctgacaagta gcgttcatcg actttcactg
    10321 gttctgactg accaatcaca tctactggaa attcttcatc tggtgatgtc cgttctgggt
    10381 atgcatcccc gtagtttatt ttcaacgctt ctgcattgtt aaatgacgta tttaatacaa
    10441 tagagatatc tttagtgaca aattcgccac cttcttggtc aagattggta aacttcaatt
    10501 gcttatcatg cataacagcc gttgttgttt ggccgccacc catatcaatg acgatcgtac
    10561 caaaatcttt ttctccgtca gaaagaattg attcagtcag cgctaaagga gtaatcacca
    10621 tttcgttgac gattaatccg gcattttcca cgcatttacg tatgttatgg atgatcgttt
    10681 taggtcctgt aaacaacaga ccgtacattt ctaatcttac gccgatcatt ccacgaggat
    10741 ctttgattcc ctcgaaccca tcgacagtaa aatcttgtgg caatattgac acgatttgac
    10801 gttcaggagg tattgaacga acaagggcag ctgaagccac atttctgaca tcttcatcgg
    10861 tgatttcctt tgaatctccg ttaactgcaa tcattccctg gcaattttct acttcaagca
    10921 tgtttgctgg caaaccgaca cttacgcctt tgatctggat acctgctttt tcttctgctt
    10981 gtcgtactgc tcgttgtatc gcttggaccg ttttatcgat atcaacgata atgcctcggt
    11041 taatcccttc tgattttgcg tttcctactc caataatatt catttggctg tcgatatatt
    11101 cagccacgac aactttaaca gatgtcgttc caatatcaag gcctacatac attcctgttt
    11161 ttgccatgaa tgggattccc tcctacttac tattccatat acaagcgaac aactagcgtc
    11221 cgttttaaaa tatacttatt aatagatata ttcactgctt ccaattctac cataaattac
    11281 tggtttttag aaagtacatt cttacttttt aaagttgttt tatcaatttt catttaaact
    11341 tgacggagtt gtacttggat tatcttctat ttgacttgtt tcacttgaat catttgaatc
    11401 agatgatccg tttgtgctag acgatccagg tgtttgtgct ttttgcgttt ctgcctcttc
    11461 acttgattcg tttgtttgat ccgcattttc ttcactttca gttgtttctt ccgattggcc
    11521 cgattcttcc gtttcttgag tgctaccgta aggatgcgag aagataccaa cttccatgtc
    11581 gatcacacct ttttcatcca tatctttggc tacttgcgaa taatatttca tttggttcgc
    11641 aagattattg atatttacaa taacttgatt gccgtcattc atattcaatt ggatcagatt
    11701 tttattgctt gctcttggcg tgtacttgat ttcagaaatt gctttttgta attctgatga
    11761 taactgattg tattgctttg ttaattcttt gatttttgca gagtctttga actcttccaa
    11821 gatagggaga ttttttgtag gatttgctac tttttcatct aatactgttc cattttcaat
    11881 cactgggtag tattgattgt tttttgcgat caaagcaatc tctttatatt ctgttacatc
    11941 caaatcgaat tcattcatcc ctttgaaatg tatatttgct gtttcgattc ttggctgtgc
    12001 ttttttaagg cgatcgatat aagtagatcg atgaaaatac tgtccccaaa cattttctcc
    12061 taatgacaat tttgtatcag agatgatttc ttggctgttg actgtcttat tgcctgatac
    12121 tgcaactgct tgtaatttgc tataaggaga aacataatag atcaaaaaaa tcagcggtat
    12181 acccaataca gtgatgatca aagctaaccg acgaaatagt actttgtgcc gttgactttt
    12241 taaattgggc agccggttca aaaaagaacc gttatatggt ccaccgtttt tcttttgctt
    12301 gatctcttca aaaaattcga tttttttctc ggactggctt tcttcttgct gttcctcttg
    12361 aaccgttttt tcttcttcag aatctgaaag gggtgtatct aatgttatgt cttctttttt
    12421 tggctcttgg tttttcatct cttgttctgc cagatatttt cggttagcct tttgccaagg
    12481 agtcaaattg ttttcatcag aagacggatg agacaatttt tttggttctt ttttcttact
    12541 cactaagacc accgccttta ttttttgatt atttgtttta ctaagtcata aagacgtttt
    12601 gaagcatcag ggattccttg ttctttagaa gctcggcaca tttgcttttg caattcttca
    12661 tcacccatga tttcgttgac tgtctgactt agtgattcac ctgttagttc attatctgca
    12721 atcatttttg ctgctcctgc gtgaacaaga ctcattgcat ttttagtctg atgatcattc
    12781 gtaacatatg ggctgggaac caacactgcc ggtaacccca gagctgtgaa ttctgcgatc
    12841 gaagttgcac cagcacgccc caccagcaga tcactggaag ccatgacctc tgccatattt
    12901 ttgatataag gctggacact aatattttgt atttcaggat ttattttatt taggatctct
    12961 tcataatatc tatctcctga agcatacaaa acttggtaag aagaaggaat atattcactt
    13021 aaaaaaactg atactgcttg attgatttta agtgctcctt gactaccgcc aaaaatcaag
    13081 actgttttct tttctggatc caaatcaaat gttttcaaaa tatctgattt tttagtatct
    13141 gctacttctt gtgctcgagg attacctaca agaactgctt tgttttcagg aaaaaaggaa
    13201 gccgcatctt taaatgaaag agcgatccga tccacatatc ggcttaaaaa tttattggta
    13261 acacctggta cactgttttg ttcatggata actgttggaa tacccatttt ggctgcagcg
    13321 tacactaccg ctccggaaac atatcctcca gtaccgataa cgatatctgg ttgaaaatca
    13381 ctgaggattt tttttgcttg acggatactc ttaagaaaca atcggaccgt cttgatgtta
    13441 tgcatggaaa tttttcgttt aaatccttga atctccaagg tatggaaagg cattcctgta
    13501 tcagggacga ttttgttttc tagacctcgt tttgctccca catacatgaa ttcagcattc
    13561 ggttcttgag tctttacata attgacaaag gcaagagctg gataaatatg tccccctgtt
    13621 cctccgcctg taaccaaaat tttcattttt tatcgctcgc ttctactttt tctattttaa
    13681 tcggttcaca gcttccatga atctgtttcc tctagtttca aaatttgggt attgatccca
    13741 gctggcattc gctggtgata ataaaactac atcaccatct gtactgtttt ctaatgctaa
    13801 aggaactgct tgttctacag aatcggctgt tactattgtc tcgatgcctg ctttttttcc
    13861 agcatccatc aacttttctt ttgtttggcc aaatacgacc attgctttga ttccttttaa
    13921 tgaaggaatt aattcatcaa atgtatttcc tctgtctaat ccgccagcca ataacacgac
    13981 cttagacgtt tcaaatccac ttagagccat tttagtcgct aaaatattgg ttgcttttga
    14041 atcattataa aattttctgc cttggatttc tcctacatat tgcgtacgat gtggtacgcc
    14101 atggaaaaaa tgcaacgttt ctcgtatagc ttcatttgat ataccataca attttgctac
    14161 agaaatagca gctagtgcat tttctacatt atgcttacct ggtacgccaa gttcagatat
    14221 ttccattatt ttttcttttt tatagtatat tgagtaatcg tcagcataca caccgtcttc
    14281 caacacttct tttgtcgaga aaggcagtac gcgtgctttt gttctttgcg ctaattcttg
    14341 tagttctgac tgattccagt taaggatcag ataatctttt tctgtcatgt ttttttgaag
    14401 gttccattta gctttgacat attcttttct tgtaccatga taatcaatat gtgcttcata
    14461 gatattggta atcaccgcta tatgagggcg gaaatctgtg attcccatga gttggaagct
    14521 agacaattcc atcacaattt tatcttctga ttttgcttct tgcgctacac cacttgctgg
    14581 atacccaata ttacccgcta aacgtgcaat tccttgatct gctcctgcat tcagtagtag
    14641 tcctgtcatc gttgttgtcg ttgtttttcc gttcgttcct gtaatcccaa taattgggca
    14701 ttccgctact tcgtaagcta gctcaacttc cgtaattacc ggaataccca tttcttgggc
    14761 ttttgctact aaaggatgac tgtagggaat ccctggattt ttgaccatca atgaaaattc
    14821 ttcatccaac aattcgattg gatgactacc tgttattact tttatcccta aagaaagcag
    14881 ctcttgtgct tccggatttt cgtcaaaagg ttttccatca ttaacggtga caagtgcgcc
    14941 caattcatgt aataatttgg ccgcacttac tccgcttttt gcgagtccta agacaagcac
    15001 tttcttattt tcatatgttg atattttttt catttcccgt catctcctac caaacgatcc
    15061 ataatgtgat caacgaagtc acaatgctga tcaaccaaaa aactgtatca atcttccact
    15121 ccgaccatcc gcacatttca aagtgatgat gaattggaga cattttaaaa attcgttttc
    15181 ctgtaagctt aaaggaagta acttgaagca tcacacttgc tgtttccata acatagatca
    15241 aaccaatcaa aagcaatgtc cattcttggt ttagcattat agaaattgcc gctaaaagtc
    15301 cgcccaacgc taatgaaccg acatctccca taaaaatctt ggcaggtttt ctgttgtaga
    15361 cgaagaaacc cagtaaacct cctaaaacac ttaagcaaat gattaataca tcatactgat
    15421 gctgatgcca agcaattaga ccgtaagctg caaaagagat acttcctaag ccagcaacta
    15481 atccatcgat gccatctgtc agatttactg catttgaaaa tccaacaagc cagaagagag
    15541 cgaaaatacc ataaatgatt cctaaaggta gatctagtcc gaagaaattc aatacacctg
    15601 gataaccttc agaacggtaa acaagataaa agatgattcc ccctagaatc tgtccaatca
    15661 atttctgctt cgagttcagt cccatgtttc ttttcttgaa tatttttata aaatcatcaa
    15721 gaaagccgat tactccgtat aacgctaata caaaaagcaa gatgaaaaga gtaggagtta
    15781 gctgtttttg ccaagctcca acccaaattc ctgtcaaaat agagccgatc agaaacacta
    15841 agccgcccat tgtaggagtt ccggctttgc tattgtgcca ctttggtccc tcctcacgaa
    15901 tcgcctgacc ttgctttttc atttgaaaat aaccaataaa tagcggcatt gcggcaattg
    15961 tcattgcaca actgctgaca atgggaatca atgcttgtgt ccattccatg ctcttttctc
    16021 tcctatttct cttcttattc ttctaaagta aaactaagtt tgtcttcagt gattttttca
    16081 tatggagcaa gttcttgttt gacacaataa ccatctccat caaaactaac ttcaataccg
    16141 aggattttcc ccagcttcat gatatctgct tttgaccatc ctgttacgtc aggcatcagc
    16201 ttatcaccac cagtatatag gatcagtttt tctccagaga tcaactgatc accgttggca
    16261 gtagattgtt tttgtacttt tttaccatta ccgatcacta caggttgcag tccacttttt
    16321 tgagcatcag ctgctgcaac gtccgcttca agattacgat aatcggctac ggatactttt
    16381 tctgtttttg tttctgtatc tgaatcttct tcagtttctt taaaatccat tgcccgtttc
    16441 atcaatggat ttgcgatttt agccaatgcc attcgatcat atgtcttagg gtgtttcatt
    16501 gttagataca agacatagtc tggatcttca gaaggaacca tctccacaat cgaatacagg
    16561 taggctgtat ctcctgtttg atatccccct gtatcagaag cgatttgggc tgttccgttt
    16621 tttgccgata cattgtatcc aggtacacta tatacaccat aagcactacc gtagttttcg
    16681 ctttcgacaa catctctcat atattcacgg actttttctg ttgtttcttt tgaaaaaggt
    16741 tgccctagaa cttctgtttg agttgttcgc tcttctccag tttggggatc caccactttg
    16801 ctgatatatc gaggttgaat cattgtaccg ttattcgcaa tagaagtgaa agctttcatc
    16861 atttggaaat tcgtcacacc taccgcttgt ccataagcac tcattgctcg gtcgacaata
    16921 tttaatgtag gtaaagcacc gtttacttca tcatctaatc cagaatgagt actttgtcca
    16981 aatcccagtt tttgtaaata gttataccat cgtccgccta atcgctgttc cagtatgacc
    17041 atcccgacgt tacttgacca tgaaagagct tgacgcatgg tcagcacacc tttttcacca
    17101 aagtcatggt cattgatcgt agcatcttct acttggattt ttccagattg gaaggtttca
    17161 ttctcgttga attctccttc ttcgatagca gctgcagtag tgaaaacttt catggttgat
    17221 cctggttcat aactatcttg taccaagaaa ttacgccaga tagcatcttt tccagtcaat
    17281 ccttccattg tttcaggatt gaaagtaggt cgttgtccca ttgccaagat ttctccggtt
    17341 tttgctttca tcaggactgc tgtcaattct tctggctgat attcttcatt tacttgatcc
    17401 atcagtgttt ctaaataact ttgtagtctg ctgtctaaag tcgtgtagat atcttgtccg
    17461 tcgacagctt tttcttcttc tgccactgta cctggcagag gattttgaaa attatctttt
    17521 tgataaataa tcttaccatc tttaccgctc aagatatcat tgtaagcaga ctccaaacct
    17581 agtttaccaa ctaagccatt ctcatctttg tctggaactg cgtatccaat aaaatgagaa
    17641 gaaaagacgc catttggata catccgagcc tgatggtcga caaaataaag accagcgaca
    17701 ccttcttttt tcatgtccgc ttcgatgttt tgcttggttt cttgggagat atttttccca
    17761 tagctaccaa attcaacttg atacagctct ttttttgcgc cgtcctctaa aatattcagt
    17821 gcatcttttt tgtctataga aagattctta tgcaggattt ctgctatttt atcaaaattc
    17881 ttttcttcaa catacagttt tttatcccca gaggtatatg tttttgagag tatcgcttta
    17941 attgaataag aggaggcatc ttctgcgagc gccacaccgt tacgatcata aatcgttcct
    18001 ctttttgctt ttactacttc acttccttgg tacaactgtt ttgttttttc agctaatgag
    18061 gtaccagcaa catgacctcc aataacgata tacgagaaac ggacggcaaa taaaaagaac
    18121 aatccaatac tcgtagcaaa taaaatgata cctacttttt tgcgattgtt catcggattt
    18181 agatttttct tttccataaa ccgccgaatt ttatttttaa gactcattta cttcactttc
    18241 cttaaattgt cgtcattaat agacaatcct ttttcttctg cgatttgttt aatccgttca
    18301 gtacgagaaa gttcgttttt ttcttgttct aaactcgttt tttcttgctt tttatcattg
    18361 atttctgttt gtattgacgt gatttctttt tcagctccgc tgatatttgt gcgcagcata
    18421 actgttaaaa cacataaagc aacaatagca atcgccacgc ttgccacagc aattttttcc
    18481 aaagcagaaa tctgctttaa tcgttttgcc ggtgattgtg gaagaatgat ttcttgattc
    18541 gaagaagaat caggcatttg ttgttccggc tgatccacca attcaggtaa atcatattcg
    18601 taatcttgca ttttctttaa ttctgccatc attctactcc tctttcattc ttattttttc
    18661 tgcaatacgt aactttgcac tgcgtgatcg attgttttct gttagctctt cctctgaagg
    18721 aaggattggt ttacgagtaa tgactttcag ttctggttgg aattcgtcag gtatgacagg
    18781 aagtcccggt ggtagatctt gtaccgtact atattcttta aacatacttt tgacaattct
    18841 gtcttccaat gaatggaatg tgattacgct gattcttcca ttgactttca ataaccggat
    18901 tgcctgttct aaagaggcct cttcggcacc gagttcgtca tttactgcaa tccgcacagc
    18961 ttgaaaaatt ctttttgcag gatgcccgcc ttttcttctt gcgggagctg ggatgacttg
    19021 tttaatgatt tcgacaagct caccagttgt ttggattggt tttgtttttc gtactcgttc
    19081 gatttcacga gcaatttgtt tggaaaattt ctcctcgcca tagcggaaga aaatctttac
    19141 taattcatgg taggaatatt cattaattac gtgataagcg gataaaggag ctgactgatc
    19201 catgcgcata tccaacggtg catcttgatg ataactaaat ccacgttctg cttcatctag
    19261 ctgaggagag gaaaccccta gatcatacaa aatgccgtct accttatcta catgctcact
    19321 caagcgttct tctaattctc tgaagttcgc tttgataaat gtgaccatac cttgttcgac
    19381 ataagaagct aaacgcagct tggcgtgatc gattgctttt tgatcttggt caaacgcata
    19441 cagatgccct ttagaactta gttggcttaa taaatattca ctatgtccgg caccgccgag
    19501 tgtacagtct acataaacgc catccggttt gatgttcaat ccatctaccg tttctttcaa
    19561 catgactgta tagtgttgaa atgtttcagt cattagtcag cctcttccta ttcttatagt
    19621 ccgaaatcaa tcatactttc agcaatttcg tcaaagttct cttctgctgc tgcagaaaaa
    19681 tcttgccatc gtgtctcatc ccaaatttca attcggttag atacaccgat gactacgcaa
    19741 ccttttgtca aagctgcata atttcttaac gtgctgggta tattgattcg tccttgttta
    19801 tcaagttcgc attcagtggc agccgaatag aagaatcgca caaaagtacg tgcgtctttt
    19861 ttagccaacg gcatttcgtt cagtttagct tccagatttt cccattcgct catcggatag
    19921 ccgaaaagac agccatccaa tccgcgtgtc agaacaaact tttcgccaag ttcttcacga
    19981 agttttgagg gcacgattaa acgtcctttt gcatcaatat tatgttggaa ttcacccatc
    20041 aacattgaaa gccccccatt cctccactaa ctaaaattag tctaccacaa tcccccactt
    20101 tctaccacct tttctgggaa atccgggaac aacttttttt attcataaac atttttttaa
    20161 aaatcaagct gcaaaaagcc ttttaaaaga ctttttacag cttttctatt tttttcatag
    20221 gtaagaaagt atgttcacaa tgatcaagac agcgtacaag acgattgtca acagaaaaac
    20281 caaacgccaa gtcatcttga aataacgcgg ataaataatt tcttcataat aatatgcttg
    20341 gaaaacagca atacaaattc ccattagcaa aacagaaata aagaaaaacg gtaagataga
    20401 aattgtccct gtatttctag acaatgcatg cataccgccc cacaaaaaag gaatcgccaa
    20461 atcaggggct tttattttaa aacgacgatt taacgaaaat aattttacaa gaaattggca
    20521 accaaacaaa acgattaccg ggtaaatata ccaaaacaag ataagtggtg tgaatgtgcc
    20581 catgaacgta aactcctttt ttatcgttgc ttcaagtata ctgtatatac tttacacaac
    20641 tgtttcattt tatttaaatt tatgcagtta tattctatat cttacaaaca cattccatgt
    20701 attccaattc cctatccgtt ataataaaag aaacaggcaa caggaggaaa atcatgatca
    20761 aatattttac attagataat gagaaactgc gtaataccgg aaaggaaact gaccaaacga
    20821 tctggtattg tgtagaacgt cctaatcaag aagagattca tcagctgaca catcaatttg
    20881 aaattccgtc agattacatc acttccatat tggacgatgc agaaaattcc cgtacagaag
    20941 aattcaatca atcaaagttg acaaagccag cgttattatt gttacaatat ccttttgcta
    21001 aaacaagtcc tagcggttat tttcaagtgg atacataccc gctctctatt attttgacgc
    21061 ccaaaaagaa gcttatcaca attacgaatc atgaaccact gtttttaaag aaagtcttta
    21121 gccagacctt tccaaaaagt gatatttcgc ctaatctcaa tatcttcttt caattcattt
    21181 ggcaggtcac actctcttac aacagttatt tagctacgct tcaatctcaa tgcgatactt
    21241 tggaagccaa acttcaagta tccactgaaa acaaacaact ttatcaaatc atggatatcc
    21301 aaaaaagcct cgtttatttc agagaagcga cttctgctaa tttagatgca ttgaaagaac
    21361 tagcgcaaaa taaacttttc caagaaaatc atgctttgaa aaatcattta cgtgatgtat
    21421 tggtggaaac agagcaggca atgactactt ctcgcattca attaaaacta gtagaacaaa
    21481 tgaatcagac tttttcagca atcgtatcga ataatctgaa taatattatg aaaattttaa
    21541 cttctttaac gattatttta actataccga caatcgtcgg cagtatctat ggaatgaaca
    21601 ttaagttacc tattgctgaa agggatgatg cctttatatg gctagttcta ttaacattat
    21661 ttatttgtat cgtaactacc tattatttaa aaaaagggaa gtttttataa aaaagccctc
    21721 ttgtggaaat ggaaaaaaga ttgtacactt gaatcaacta attaatgacg aggttgaaaa
    21781 caatgcgtga aaaaaaacaa acaagtactt ttgctaaagt aacaaaattt gtcatttgga
    21841 caatgctgat tttaacgatt ggatctgttg tattgactgc tgtaatgagt gtcatgtaaa
    21901 aataaaatat agctatcagc tgctgaaaag gacctgaacc actgcgaggt ggctcaggtc
    21961 ctttttgcga gtttcaatag ttcttttgca tgttcgagtg tgagttttgt aatctcactt
    22021 ccagccagca tacgtgcaat ttcttcttct ctttcttttg ttttcaactc tgaaacagaa
    22081 gtttgcgtac gtccatcaat cactgctttc acaatataat attgatagtc ggcgactgca
    22141 gcaacttgag gcagatgagt gatgcacaaa acttgagagt atttcgatat ttgagaaatt
    22201 ttatctgcga tggcctgtgc aactcttccg ctcacacctg tatccacttc atcaaaaata
    22261 atacttgtca caccttgctc agaagaaaaa atagttttca aagcaagcaa catccgagac
    22321 agttcgccac cagaagcgac tttcaccaaa ggcttcagtg gctctcctgg attggtcgta
    22381 atgtaaaatt cgatttggtc aaatccatca tgattcaatt gtctattttc ttcttttaag
    22441 aaacgtacct caaactcagt gttttccata tacagacttt tcaattcatg cagaatggat
    22501 ttttccaatt cttttgcgat ttttttacgc gcttcatgaa gatctgcggc ttgttgctga
    22561 agtagaagct ctttttgctc tagttcctct ttcattttgt ccaattgacc ttctgaaaaa
    22621 tcagaagaag ctagctcttc ggtgatttcg tcatagtaag ccaatattga ctcaatggat
    22681 tcgccgtatt tacgtttcaa ctgtcggatt aattctaaac gttgcgtaac ttcttctaaa
    22741 cgactctcat ctaactccaa taaatcaatc tgacgactca tatcgccgat tgcatcttgc
    22801 agcaaatagt aagcactttg gatattttca taaattgctt catactccac atctagatgg
    22861 gcgatccctt gcagttctga aacggccaag ccaacaccat ctacactgtt ttgttcatta
    22921 ttcgtcagtg ctccgtaacc tgcagctaat ccatcagcaa ttttttgata attgctaagt
    22981 ttttctcttt cttcttttaa tttctcttct tctccgactt gcaaatcagc agcagctatt
    23041 tcttcttgct ggaaatggag catatctatt ctctgaacat aggatttttc attttgctgc
    23101 acttttcgta cccttttttc cagctcacga taagagatat attcttgctg ataactcttt
    23161 ttcttctgtt ggaaagctac atctccaaaa cgatccaata aagcaagatg tgattcaggc
    23221 tggagcagtt cctgatgttc gttttgtcct tggatatcta ctaaataact gccgattctt
    23281 ctcagattag ctaatgtgat aatatgacca ttcactcgac aaacattttt tccagttaag
    23341 gacatatccc gtcgaacaat cagattgtcc tcatctgttt cgatccctaa ttcaaccatc
    23401 agttcagaga atccttcttg ttttggtagt tcaaacaacc cttcaagcac acatttttct
    23461 gctccttgac gtatataatc gcttgaacct cttcctcctg ctaacagacc taaagcatcg
    23521 ataataatgg atttacccgc accagtctct ccggtcagcg cggtcattcc ttcgtgaaaa
    23581 gaaagccgca attccggaat aatcgcaaag ttagtaatac tgatttcttg tagcatcaca
    23641 ttcgctcctc aacttaaagg taatgcagga agtctttctt caataaaact gcctggtctt
    23701 cagttcgtgt aatcatcaaa acattatcgt catcattgat aatcgaaaat aattctttct
    23761 tgtaacgttt atcaatcaaa tttgctgctg ccgaagcatt tcccggcaat gttttcaaaa
    23821 taacaaattt ttccatttgg tcaactgcca cgaaagcgtc ttttaatagt ttttccaatt
    23881 tagtatttac atcttcgttt gtttcagctg gtatgctata acggtagccg ccatctgaag
    23941 ccggtacctt gatcagtttc agttccttta tatctctgga aatcgtagct tgagtaacag
    24001 atattccttt gcttttcagg atttcaacaa attcttcctg ctttcggata tcttgctcac
    24061 tcaacaatcg agtaatcaaa cgatgccgat ctttttttct cattacgtct tcgctcctta
    24121 gcatattctt cttaatcaat gatagcttat ttccttggta taataaagaa gaatccgcat
    24181 acttctcact tgattttcac agttacaatt tatttatggt caaactgttc gtgtgcattt
    24241 gaaaccactt ctgccatatt aatagctgaa ttgattgttc cgctttcggg tactttcctc
    24301 aagtgagcca agaactcgat atttccttct ccccccgtga tcggagaata acttaattcc
    24361 agcacatcat atccaccttg tgccgcaaat gacaaaatgt cttctaaaac tttttgatgg
    24421 gtctgagggt cacgaacaat tccgtttttc cctacagctt ctctgcctgc ttcgaattgg
    24481 ggtttgatta aagcaatcac ttctccctct tcttttaaaa tagcatgtaa tggaggcagc
    24541 atcagtttta gtgaaataaa agaaacatcg ataacagcaa cttctggaat tcctaaggta
    24601 aagtcttcgg gttttgcata acgaaaattc actctttcca ttacgacgac tctaggatct
    24661 tgtcttattt tccaagctag ctgattgtag ccaacgtcta aagcataact catttttgcg
    24721 ccattttgca aagctgcatc agtaaatccg ccagtggatg caccaatatc taacaatgtt
    24781 tttccttcaa cagatagatc aaatgctttg agtgcttttt ctaattttaa tccccctcga
    24841 gaaacataag gaagtttttt acctttgact cttaattcac tactcactgg aattttttct
    24901 cccggtttat caaaccgctc gtttttttcg ttgtaaatca aaccagccat cactgaacgt
    24961 ttggcttgtt ctcttgtttc aaataatcct tgtttgaccg ctaatacatc tacgcgttct
    25021 ttttccattc tttccctcat ttctccacat caaattgctt gatcatctgg cgcatcagcc
    25081 cgccatcaaa agaaaaaacg tcctcttcta atttgtctat gatcttgttg gcggataaca
    25141 gctcaatctc caacgcctca cgtgtctttt ctaggcctaa taaacgcggg taagtatttt
    25201 tttccatacg ttcatcttta ccagctgttt tccctaatgt tttcgttgta ccaatcacat
    25261 cgagtagatc atctcttatc tgaaaagcta aacctaaatg ttcggctaat cgttgaagta
    25321 gcaataagat ttcttcaggt tgcttcgcta atatgccacc agccagcaag gcataacgaa
    25381 tcaaatgccc tgtctttctt tcatgaataa atgctaattc ttctaaagaa agctttttcg
    25441 attcgccctc gatatctgca gcttgcccag caaccatacc ttgtgatccc gcacaaacag
    25501 ctaattgctg aagcagcaga agcttaggag aattacctaa gtgtgccata ctgattagct
    25561 ggaatgctcc tgtaagcaaa ccatctccgg ccaatatagc taatgcttcg ccgaatactt
    25621 tatgattggt aggttttcct ctacgaagat catcattgtc catcgcaggc aaatcatcgt
    25681 ggatcaagga atacgtatgc accatttcta aagcagctgc tacttgatag acgggaacat
    25741 caataggctc atcaaaggaa gcaacggccg ttaaaagtaa taagggacgt attcttttcc
    25801 cgcctgcttt cactgaataa ctcatcgcat catagagtcc tctatccgtt gtatgctcat
    25861 ctaaaaaaga caagatttct ttttctacag caggtagatg aatagctgaa aagtcagtta
    25921 atctcatctc agctttcctc gtttccgtca aaatcaactt cttcatttga ttctgtcatc
    25981 attttcgtca aggtctcttc tgctttagct aatgtatctt ggcaatgttt gcttaattcc
    26041 attcctcgtt taaacgaatc tagagctgat tctaatggaa cgtctccttg ttctaactgc
    26101 atgacaattt tttctaattc ttgcagtgac tcttcaaaag ttggattttc catattttta
    26161 ttctcctttg tctatctctt gtacttgtcc tttaacgatt ccatcttgat aatggatcgt
    26221 cagcaaatcc ccttgatgaa tctcggtgac actttttact acttgatcat ttaacgtcgt
    26281 atagctgtag cctcttccca tgattttcaa tggactcaac aaatcaagag attgaacagc
    26341 ttgagcaaaa cgctgctgct gttgtttcat atattgttgg attttctctt ctaagcgttt
    26401 ttctagataa gcagtttctt ggcgagctgc tttaactcga taaagcggag ccacttgttc
    26461 caattggtgg cttaatgcga tcgtttgttt ttctttcgca tgataaattt gctgtgtagc
    26521 ttgatataat cgctgtttca actggtctaa tttgatggtc tgaccttcat acaatctttc
    26581 aggctgccga aatacatagg aacgtcgagc acgctcgaac cgttcttgtt tgcgctggat
    26641 ctggcgtaca aacgcctgct gcaatctagc ttgccgttct tgaatgcgca tcaactcttc
    26701 tgacaaaaca ggaacagcta attctgcagc tgctgttgga gtggcagccc gaacatctgc
    26761 tactaaatca gcgatcgtgg tatctgtttc atgtcctaca gatgaaatca ccggcgtatt
    26821 agcttgataa atcgcccgag caacccgctc ttcattaaac ggccataaat cctcaatcga
    26881 accaccccca cgaccgatga tcattgtatc gaaattgccc atctcttcta ctctctggat
    26941 attcctgaca acattgtccg ctgcttggtt tccttgtaca actgttggga acaaaaccaa
    27001 ttggacaatt ggatatcttc ttttcaccgt cgtgatgata tcgcggataa ccgctccgct
    27061 tggactggta aggaccgcga ttcgctttgg gaaacgtggc agcatttttt taggcccttg
    27121 aaaaaggcct tccttcatca gcttttcttt tcgttcttcc aattcttgat ataaagcacc
    27181 tattccatca ggctccatgt gctctacata gatttgataa gaacctcccg cttcatataa
    27241 tgaaatacgc ccaacgacaa ggactttcat tccttctttt ggctggaatc gtaatttttg
    27301 gaatgctcct ttgaacataa tagcagatat ttttgcatgg tcatctttta agctgaaata
    27361 ttgatgggca ttggcacgta aacggaaatt cgatatttcg cctgttaaat atacgcgttc
    27421 taaataagga tcggcatcaa attttctttt caaatacttc gttaatgtag tgacagttaa
    27481 atattcttgt gccatctatt cacctgctcc aaaagcttcg gcagatttta cggtttgata
    27541 catcagcatc gtgatagtca ttggccctac accctttggc acaggagtga tccagcctgc
    27601 aataggctcc acttcatcaa atttcacatc accgatcagc ttaccgttag catcacgatt
    27661 catccctaca tctatcacta cagcacctgg ttttacgaat tcttttgtga caaaatgtcc
    27721 acgtcctatt gctacgacta aaatatctgc ttcttgtgcc agttcagcca gattttcggt
    27781 tttagaatga gcaacagtaa cagttgcatc tgccatcaag agtagctgag ccatcggttt
    27841 accgacgata ttgcttcgtc cgataactac tgctcgttta cccgccggat cgatagaata
    27901 agcttcaaac attttcatga tgccgtatgg agtacaagga atcttgtccg gtttacccgc
    27961 aaggagtctg cctaaattca tcggatgaaa accgtctacg tctttttcag ggcggatagc
    28021 taacaggatc ttttcttcat ctatatgctt tggcaatggt aattggacca atataccatg
    28081 aaaactagga tcttgattgt atttttcaat ttctgcaagt agttcgtctt cagaaatcgt
    28141 ctcaggataa cgctctacca atgaatggat tccgatttct tttgctacac gttctttatt
    28201 tcttacatag atttggctgg ctggattttc ccctaccaat aaaacgacta atccgggatg
    28261 gataccatta tctttcaatt tttggatttt ttcagcaatc tctgcctgca ttttttcagc
    28321 taattctttt ccgttaatca attctgccat ctaaccactc ccagatattt tttgtgtttt
    28381 cattctacca tagaattttc ccgatttata acaggtttat gatgattcat agaaaaaagc
    28441 taggacttac gccccagctt ctaattcttt caatacattg gataaaacac cgttgacgaa
    28501 tttacgtgaa cggtcatcac taaatgtttt tgccaactcg attgcttcat ttaaagcaac
    28561 agccgctggt acatctgtca cataagtcat ttcaaaaatc gcgatacgca agatcaccaa
    28621 atccattttt gagatccgat tgatgtgcca attgtttttt aaatgttttt caatcacttt
    28681 atccaattca tctttttttg cacagacacc gccaactagc gtatcaagat aaacaggaac
    28741 aaactctgat tcatcttcgt tgatcatgtc gcggtggtct aattcgattg cgttgaaaat
    28801 cgcatcttct tttgttaggt ctgcattgaa gtctaatgga aataaagcct gcaatgccat
    28861 ttcccgaatc tcgtgtcttg ataattcttt attcattctc ttcctcttcg tcttcaaaaa
    28921 gttcttcgat tgttggttgt tctaattttt caggaacaac agctactaca tggatattga
    28981 cttcagataa atcgatatcc gtcatgaaaa ggacttgctg gctgacccgt tcctgcattt
    29041 ccattgctac tttaggaact gaaacaccat attctagata acagtaaaga tctactttca
    29101 agccatcttc ctcgtttgct aagtaaacac cttttccgtg agctgcacgt cctaataatt
    29161 cagtgacatt gctggcaaaa gttccgcgca ttccgtatac gccttctact ttagaagctg
    29221 caatcccgat aattacttcg atgacttcag gagcgatcac gatctctcct aaatcttggt
    29281 ttgcgtttaa aatcaaattt ttttcatctg ccatttattt attcctccct ttgcctaagc
    29341 acgagataca tccgaattct ctatgatgcg gcatagtgat cacgtggtaa gaaaactgcc
    29401 gccgatcagc tgcaaactgc tatgaattgg agaaaacacc caactttatt atctcttaca
    29461 gcttaaaagt tcttctatag tgtatcatta aatcgagtga ttttctataa caaagtctgt
    29521 gaacactgat ttttacgctt acttcttcta ttgatgaaac tataagatga tcaattcttt
    29581 tggtgaacga gtcaacacac gatttccatc ttctgtgatc aacaagtcat cctcaatacg
    29641 tactccaccg attcctggta agtagatccc tggttcatca gtgatcacat ttcctggtac
    29701 aaattgcttt tctgcacgag aagaaacgtt tggtccttcg tggatctcta aaccgatccc
    29761 atgtcctgta gaatgtccaa atgcttcccc gtaaccatgt tttgaaatat aatctctagc
    29821 aactgcatct aattggacac cagtcattcc tggttttgca gcatcaataa ctgctaattg
    29881 agcatctaag actatttgat aaatttcttt taatttttcc ccaggatcac caatagcgaa
    29941 tgtacgtgtc atatcggaaa cgtacccttc gtaatagcaa ccaaagtcca atgtgatcaa
    30001 gtcaccttgt tcgatgattt ttttgcttgc tacaccatgc ggcatagccg aacgcaatcc
    30061 gctagcaacg atcgtatcaa aagaaacacc tgaagcgcca agagaacgca tataaaagtc
    30121 tagttgattt gcgacttcaa tctcagtcat gcccggttgg atcattttca ggatgtgatc
    30181 atatgccata tccgcaatat gacatgcttt ttcgataata gcgatttctt cttcatcttt
    30241 cacttctctg agttcttcga tcatctgtga aacaggtact aattccgcat cgatgatttc
    30301 ttctaaaacc gaatattcta aaaatgagac tgttgtttcc tcaaaagcta atgagtgcag
    30361 ttcttctttg cgtactaagt ttgctacctc atcaaaaata ggtccgacat ttttaataat
    30421 ttcaaaccct tgagcctgcg ctgccgcctg ctcggtataa cgaaaatcag taataaaaaa
    30481 tgctttttcc aaagtaataa cagctaatcc tgtcgtacct gtaaagttcg ttaagtaacg
    30541 aagattgtac ggactggtaa ccaaaaatga atccaaattt tcttcttgca tttttttgcg
    30601 caatttttct acacgtagca tcataatgaa actccctctt tctttgcacg cttttttcca
    30661 ttatagcaaa cttttcttcg tacacgacag ttaaataaaa atttccggaa aacttctttt
    30721 tattttaaat aaataaagcc aaaaaggagt cacagtctcg ctgcaactcc ttttagttta
    30781 ctattttgta aaaacttatg atcgttttcc aaagaaaaat gagacaactg ctaccaaaat
    30841 cactgcgcct aaaatagatg gaataagtgc catacctgca gcttttggtc cccattcgcc
    30901 gaaaatcgct tgtccaatac ttgaaccgac tagtccagca atgatattcg ttacacagcc
    30961 catcgaattt cctttactgg tgatcgcacc tgcaattgct ccgatcacgg ctccaacaat
    31021 caatgtccat aaccaactca taatataacc tccttctttg ctaatcaatt ttagcatatg
    31081 ggatttaaag gggacaaata attggttttg ctatttcata ttttcttctg caaagtaaaa
    31141 aaataaagaa aacctcctct tagaagtttt ctttattttt tacttgcgtt taaacatact
    31201 acgaatacca cgtttcacta catcaaagaa actcaatgat tgtaccattc tatcttcatc
    31261 tacatattct ctgatcgcac gaagcacttt tttaggttct ttggaagaaa atgtgtatgt
    31321 accatttttt ttcgtttgaa tggcatatcg aggaatccat tttcccttta gcatgactga
    31381 tgctagtaca taatccactt cttcccatgg aatttggata tatttgcgtc tatctcgcgc
    31441 atggtaaaac tcaaaaccat tatcacctat catgatttgc ccgtaatctg tcagacccgt
    31501 aaacgaagta gcatcaatta ccaagtctac ttttgtattg attgattgaa ccattttctc
    31561 cctccagata tttgtttatg aacagtatac tatataaaaa aagctcggga caacgcccga
    31621 gctatatttt tatggattat aaaagtccga tgacgtgtcc aacaacaccg actacgaata
    31681 aaccaaggat gataacgatt ggagaaacct ttttcttaag caaccacata cataacaatg
    31741 ttaatcctaa tgcagctaaa ccaggaatca attggtctaa gttgtcttgt aatgttgtta
    31801 ccttttcagg agaaagggct aagccattat taacttgttc aaatgcactc ttgattcctt
    31861 caccacctga aggcaattta tcccattcga tgtaagcacc tttatctaat tttacagttg
    31921 atacaactgg tgagaatttg attgataccc atctttgtac aagggcagct aatacgaaca
    31981 tccctaaaat tgaagcacct tttgttacgt cttgtaacaa tccgccagaa aggtcatcag
    32041 taattttaga accagctttg tagccaaatt cttgtgtata ccacatgaat gcccaacgaa
    32101 taatgttcca acctaagaag aagatgattg gtccaaggat atttcctccc atagctaatg
    32161 aagcacctag tgcacctagc attggacgta ctgtaaacca gaacactggg tcaccaacac
    32221 cagctaaagg acccatcata cctactttaa ccccttgaat cgctacatca tcaacaggcg
    32281 cgccattcgc acgttcttct tccagagcca atgtaacccc taaaattgga gaagcaatgt
    32341 atgggtgtgt attaaagaat tccaagtgac gttttaatgc tgcagaacgt tcttcttttg
    32401 ttttgtataa ttttttgatt gctggaatca tagaaaatgc ccagccaccg ttttgcatac
    32461 gttcgtagtt ccatgaacct tgaatgaagg tagagcgcca cgcaacagct aaacgatctt
    32521 tttttgataa tttgattttt tcttctgcca ttttattttc ctccttcaag attaatagtc
    32581 attcaaaatg tcgcctaaag ggtcaccaga gttgcttccg cctccattat ttgatgaacc
    32641 acccatttta gaaaggttca agtaaataag agctaatgca acacctaaag cacctagagc
    32701 aatcaatgtt aattgagaaa ttgcagctac aacgaaacca ataacgaaga atggccatac
    32761 ttcttttgta gccatcatgt tgattaccaa agcgtaacct acagctacga ccattccccc
    32821 accgatagcc ataccatctg ttaaccatgc tggcattgat tcaaggaatg attgcacagt
    32881 ttgtgctggg ataaatagca acgctgctgc tgggaccgcg atacgaaccc cttgtaaaca
    32941 tacagcaatg atgtgccaca tttcaacctt acggatgttt ccttcttcag cagccgcatc
    33001 catcaaatga acgattggaa cagcgattgt acgtacgatc attgttaaga acaaccctgc
    33061 aacagccaaa ggaacggcaa tcgcgatcgc agaaggaaca cctttaacac cttgtccacc
    33121 taaaactaaa ataattgctg atgctactga tgctaatgca gcatctggtg ctacagcggc
    33181 accgatattt gcccaaccta aagcgatcat ctgaagcgta ccgccaagta cgataccagc
    33241 ttcgaggtta ccggtaacta aaccgatcaa tgtacatgcc actagtggtt gatgaaattg
    33301 aaattcatca agaatacctt ccataccagc tagaaaggca actaatacga ctaaaattat
    33361 tgaaataata gacatgataa gcctcctata aattttaata ttatttatta cgcttttgct
    33421 aattcagctt ttgctttttt caaaatatca tccatgttat cgcgtgaatc attaggtact
    33481 ttacgtacat cgaatttcac acctttttgt tcaagcttct caaacgcttc aacgtcttct
    33541 tgtcccatag acaatacttt acttacaacg actttaccga ccgaatgcgc cattgaccca
    33601 acatttaatt ctttgatgtc tacgccacct tcaacagctg tcaatacgtc ttcaggattt
    33661 tcaaataaaa gcaatgcttt ggtatttcca aaacgagggt cttttgctac ttcgatcatt
    33721 ttgctaatag gaataacatt tgcttttaca cctggaggag cagcttgttc gattagtctc
    33781 ttacgcagat catcctttga tactgcatct gaaacaacga taatacggtt tggttgcact
    33841 gcttttgtcc aagctgtcgc aacttgtcca tgtaacagac gtgagtcaac acgtgctaat
    33901 acatatttga tttttccgtc tccgaccaca gttccttctg gaagtgctcc tttaggttgt
    33961 gcgtcttcaa ctgctgcttt cggtgcttct gccggttcta attcttccgg ttttactttt
    34021 acaccgtctt ttgctgtttc aatgatgtgt gctgcaattt catgagcaga ttccattgaa
    34081 aagcgtgatg cgtaggcttc aatcaacatt ggtaaattca aaccgctgac gattgcccat
    34141 ttatctttgt gctcttcgaa taatgtgttt gattgattga acggtgttcc gccccataaa
    34201 tcaactaaga ataacacttc gtcttgatta tcaaaagaag caaccgcttc ttccaatttg
    34261 gctctcaagt catctggacc ttcacttggt ttcaaaatca cggctttgac gttttcttgt
    34321 tcgccaaaga tcattgaacc ggattgtaag attccttcag caaattgtcc atgacttgct
    34381 aggataatcc ctaccatatt tatacctcct atttactttt gttgtaacaa attcaaaaat
    34441 attaataatt gctaaccatt acaacagaat tatttgacct tttccaacaa ccattcttgc
    34501 tacacctttt tgattttcgc tagtagttcc atcaaaagga ttttcctgtc agcaggtact
    34561 tttcgaatct ctaactccac ttcttgactg ttcaagtaac gaaaggaatc aatatcttgt
    34621 tgatttactg aaacaaaatt ggtaatcatt gatttgcctt cagtaaaact cataccgccg
    34681 atattcaacg acttcaactg tacaccgcag cggacaaccg cttcaacatc ttggggattg
    34741 gtgaataaaa gcatcacttt tgctcgtgat aataatccac tatgatagat ttgaatcatt
    34801 ttctgcacgg ttattacatt tgctgtgatc cccggcggtg cagcttcttt tagtagaaac
    34861 tttctcaatt tatcttgagc gactacatcg ctgatcacaa tgatacgttc aacacctgtt
    34921 tgcttcgacc aaactgttgc gacttgcccg tggattaaac gatcatctat acgtgccaat
    34981 cgaatatcca tctgcaaaac tccttcttcc tcactacttc acagaaaccc ctatcatttc
    35041 tgtgtgaaca accacctcct agcacacact actataagca aaaagcgtgc caactttttt
    35101 acgatacact aattgaattt aaaggattct taaccaatac acaaaaacga tacactataa
    35161 aagtgtatcg tttttgtgta ttcatggcac agtgtattct tttttatcta actgattatg
    35221 gactagacgt agcagataat acatttcttc tatcggcact tttactttta tctgatcttc
    35281 caacagaaca actgaggctg ccaattggct ataaagagga tcattcgttg tatttgtcaa
    35341 ttcgttttct tccacagtca atggttcatt ccgaataata cgttcgatca ttcctgctgt
    35401 gtgcatcact aggttgattt tgaatccata ttcttcatcg ccgattcccg tcgtctgaga
    35461 gatttgtgta gaaaattgcc acattggatc gattaactta ctaccattaa taaagatgaa
    35521 gttatcagag ataaactcaa cacacgtatt tttagctgac tcttcatcta gggagatgaa
    35581 ttcagtttcc tccaattctg attccatcag caactgatcc aaaatgactt cgatattttg
    35641 atcaataaaa cgttctaaag gaataaatgg ggcttggata ttcaaatcag taatcccagt
    35701 agtagctatg atctgatatt ctttttggat aactggcagt tcttctttca cttccacaat
    35761 cgacaaagct agaacttcta attctgtttc ttgacgcttg tttaccgctc gttcgatcaa
    35821 ttctttgatt ctttgtgctg taccttcgcc agatgcgcag atagccaaaa cagctttttt
    35881 cttatgaata ggttttgctt cctgaacaga ttctacagaa gatatttctg cgtaacctcg
    35941 aaaatttttc aatgattcat gcaacatatc tagatctgtc cctaatactg agacctttcg
    36001 tacagtttcc aaaactagag aagttgttac catatcaaca gtacgtactg gaattccagt
    36061 gtctctttga atttgcgaat taaacgacgc aagggacccc atatctacta agagtatgac
    36121 cccgcttcct tcatctactt cttgtacatt gcgttcaatt cggtccaatg ctgttttcgg
    36181 atccatatcc aaaggcatgt ctactgctcg tacgttctct gcatctaaca actgttggac
    36241 aacttggacc atactgcttg ctgtgctgtt tccgtgagct gcaacgacta caccgattct
    36301 gccgcttgct tgatttgccc gtaacgagac gagtagaacg gtcaagtaat agtcttcact
    36361 ttctggaatc tccacttgaa aatacgtttc gatataacta cgcacttctt tcgcaacagc
    36421 atattcatct ggaaaatcaa aagccatctc acggatgtta tcattcgtat gccgttcttc
    36481 tcctaagttg atttttttca aaaaagaact gatatgcaag ctcatagcat aaataaagtt
    36541 ttgctgaaac tcatatggta aagatctttt gaccattaca taaatctgat tggtcacttc
    36601 tatgattttc ggatctacaa attctgctaa tttgctgtct gcattaaaag aaaaaccatg
    36661 gtctttatag aaagacttca gatggatgtt gatatctgta gaaatgaaat gattgattgc
    36721 ttcttgatcc aaaccatccg acttcagtaa agctgcttta tctccgatga tatcatatag
    36781 attataaggt aactcatagg agtccgattg gattttcatg atcgtatcat ttggtgctac
    36841 agttatcttt ggctccaata ttttagaaag ctctgccatt gcactgcggt tgcttgctaa
    36901 ttgaatcaat ccgctacgaa tcccttctgt caaatcatcg attgtgatcg agatttcggg
    36961 tgattgcata tgatttagaa atcctcgtgc acatactaac tggatatttg acttcaattg
    37021 tcctatattt ccataggtca cactgccgat caatgctttt gctacatctt ctgtcaaaga
    37081 aatctttcgc tgcgtgcggt ttgcttcgtg ggcaatcatg atttttacta aatcgacttt
    37141 ctcatttgca ggacgttttt caaaagccgg aagtttgata ttgattggga tacgccgaac
    37201 aaaagtttct aatagagaag aacctgggtc ttctgtggtc gcacctacga tccgtacatt
    37261 tgcttcatgg gatttcgttg tttccccaag acgactgtaa gttccatgat ccataaagta
    37321 aaagatcatt tcttgacctt caggcggcaa ccggtgaatc tcatccaaaa acagcatacc
    37381 gccatccgct tgatcgataa tccccatttt ttcttcatct gcgcctgtaa aagcaccttt
    37441 tgcgtaccca aataaatgac tcatcaaaag ctcagggtta ttggcataat cagcacaatt
    37501 gaaaacaatc agttccgttt cctcatctat tacatgatgc gcttttgcaa aatgatacat
    37561 cgcgtgtgca aaataagtct ttcctgatcc ggtaggtcct gtaatcaaac aatttaatcc
    37621 tcttggcgga tacaaaatcg cagcttttgc ctgttccact gaatttttca tgctgccgtt
    37681 agctccaatg atttttgcaa agatatcctt agtatctatt tggagtgtat ttttttcttc
    37741 agagaccggc gtttctttgt aactgactac atgttttgta actggcttta cgtgcgtaac
    37801 gagtgtttga tatatgtaac gtactggtcg tccgtctgtc tttatcagtt ttccttcgcg
    37861 cactagctga ttcagatctt tactgctatt tgttcgctga atccctaaca cttcagcaat
    37921 ttcctgtgtt gttactcctt gatcatattc tgcctttgtt aaatgagcgg ttttttcttt
    37981 gacatacata taaattcggt caatgcgttt tgccaatggt atcgctcctt ttctatagac
    38041 tattgtactt aggcttcaaa acataatact atgtatttat ttctgtttcg tcaactattt
    38101 acctgatttt gttttgttga taacacgcgg atagatagaa atgttcaatt taaaagaacg
    38161 aaaaatcccg atacgatttt tataatggaa ac
//
LOCUS       DACCIQ010000019        37918 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCIQ010000019 DACCIQ010000000
VERSION     DACCIQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.019"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            548..816
                     /locus_tag="IVM62_000990"
                     /pseudo
     CDS             548..816
                     /locus_tag="IVM62_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1488..4889
                     /locus_tag="IVM62_000991"
     CDS             1488..4889
                     /locus_tag="IVM62_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6844963.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(5313..5483)
                     /locus_tag="IVM62_000992"
     CDS             complement(5313..5483)
                     /locus_tag="IVM62_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6844964.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5677..5856)
                     /locus_tag="IVM62_000993"
     CDS             complement(5677..5856)
                     /locus_tag="IVM62_000993"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844965.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(5866..6042)
                     /locus_tag="IVM62_000994"
     CDS             complement(5866..6042)
                     /locus_tag="IVM62_000994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844966.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            6243..7355
                     /locus_tag="IVM62_000995"
     CDS             6243..7355
                     /locus_tag="IVM62_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6844967.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7433..7726)
                     /locus_tag="IVM62_000996"
     CDS             complement(7433..7726)
                     /locus_tag="IVM62_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP6844968.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7894..8208
                     /locus_tag="IVM62_000997"
     CDS             7894..8208
                     /locus_tag="IVM62_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP6844969.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            8229..10208
                     /locus_tag="IVM62_000998"
     CDS             8229..10208
                     /locus_tag="IVM62_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6844970.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            10221..10547
                     /locus_tag="IVM62_000999"
     CDS             10221..10547
                     /locus_tag="IVM62_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP6844971.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10563..11081
                     /locus_tag="IVM62_001000"
     CDS             10563..11081
                     /locus_tag="IVM62_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6844972.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            11081..12433
                     /gene="celB"
                     /locus_tag="IVM62_001001"
     CDS             11081..12433
                     /gene="celB"
                     /locus_tag="IVM62_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6844973.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12517..14022)
                     /locus_tag="IVM62_001002"
     CDS             complement(12517..14022)
                     /locus_tag="IVM62_001002"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP6844974.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(14114..14704)
                     /locus_tag="IVM62_001003"
     CDS             complement(14114..14704)
                     /locus_tag="IVM62_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6844975.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14883..16529
                     /locus_tag="IVM62_001004"
     CDS             14883..16529
                     /locus_tag="IVM62_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6844976.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16578..17297
                     /locus_tag="IVM62_001005"
     CDS             16578..17297
                     /locus_tag="IVM62_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6844977.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17776..18723
                     /locus_tag="IVM62_001006"
     CDS             17776..18723
                     /locus_tag="IVM62_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6844978.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18735..19655
                     /locus_tag="IVM62_001007"
     CDS             18735..19655
                     /locus_tag="IVM62_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6844979.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19696..21153
                     /locus_tag="IVM62_001008"
     CDS             19696..21153
                     /locus_tag="IVM62_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6844980.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21498..22112
                     /locus_tag="IVM62_001009"
     CDS             21498..22112
                     /locus_tag="IVM62_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6844981.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            22105..23829
                     /locus_tag="IVM62_001010"
     CDS             22105..23829
                     /locus_tag="IVM62_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6844982.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23826..25277
                     /locus_tag="IVM62_001011"
     CDS             23826..25277
                     /locus_tag="IVM62_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6844983.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25750..27903)
                     /locus_tag="IVM62_001012"
     CDS             complement(25750..27903)
                     /locus_tag="IVM62_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6844984.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27905..28951)
                     /locus_tag="IVM62_001013"
     CDS             complement(27905..28951)
                     /locus_tag="IVM62_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6844985.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            29101..30390
                     /locus_tag="IVM62_001014"
     CDS             29101..30390
                     /locus_tag="IVM62_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6844986.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            30403..33105
                     /locus_tag="IVM62_001015"
     CDS             30403..33105
                     /locus_tag="IVM62_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6844987.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            33090..33947
                     /locus_tag="IVM62_001016"
     CDS             33090..33947
                     /locus_tag="IVM62_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6844988.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(34018..34875)
                     /locus_tag="IVM62_001017"
     CDS             complement(34018..34875)
                     /locus_tag="IVM62_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6844989.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            35086..37257
                     /locus_tag="IVM62_001018"
     CDS             35086..37257
                     /locus_tag="IVM62_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6844990.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            37325..37666
                     /locus_tag="IVM62_001019"
     CDS             37325..37666
                     /locus_tag="IVM62_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6844991.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT        12406 a         6549 c         7847 g        11116 t
ORIGIN      
        1 tttaatcaaa tagtccctac tgtgactttc ctcagctatc ggtgatacta ccgatagcat
       61 ttttttgctt ataaaataat tataagtatt tagagatttt tcttctaaca ttcttcaaac
      121 aagaaaaatc acaaaggtta ttgttatttt tatatattct ttttaagaat gattgttttt
      181 ttatagaaat tttaaaaaat aaaacacgtt attgatggat agttagtttg ttatttggta
      241 atattataga agttgactgg tagtgattta tttttcgtcg ttttataaaa atgttatttt
      301 ttggaaaaat tcacaaatat gtaacgaatg tttttctgga atttgctgtg ttaaaaagca
      361 gtatgactaa tataaatact attgtcattt atgcaatttt ttatggatag taattttaga
      421 ggggaatcaa tgaaggaaga atagaaggga aaaatagttg caaatacgcc agttagcaaa
      481 aatagcaata catcttcaag ctttaaaatt cgagagaatc atcttggtag tggaatcaac
      541 tacaacaaaa ggattaaacc aattaataga tagaattacc aatggaaaaa tagaaaaggt
      601 agttattcta tacaaaagtt gtttaattcg cttcggttat gaacttagtg aaaccctttg
      661 caatatatgg tacagaatat agaaattatt gacattacag aaaatgatga agatttaata
      721 cagattgtca ctatttgtag atacctttta caaggaaacc gagccaataa agctaagaag
      781 atgattaagg agttgatgga agacgataca agttaagaaa gtgaaactac gcccaaataa
      841 agagcaaaaa gagtaattgg acgtggaaaa tgcaagaaat gaaccatcga ttcggtggac
      901 agtttattag taatgacggt ttgagaacat ccatgacgag aatgaaggaa catcctaaat
      961 gcgtttagtt gaaccaagtg ccaaataata tggccaaata agcgatcagt tatcgatgaa
     1021 tcccaagtat tctaaactat atattcattt tgatgctaag cattgatata tttttttggg
     1081 attgatgaag gttaaccaat acagggactc actgatgaaa ttctagagtc tgaccttggt
     1141 atcaacgatt tagtgattgt ttctaatgga aaagtataaa agtatataag tacattaaca
     1201 aaataactat tagggttacg ctccagcttg tgcaaggcac tggcaggagg aatcgtgaaa
     1261 tcataattat ggttaagcca cacggacaac taaaagtcat acaaagctat cgaatcaatc
     1321 aacagaagac aatcggcaat cattagcttt gtttacagct tacattgcaa cgcttgagtt
     1381 gaaccgaaaa tacgagggag gtaacgcagc cattttgagt tggggaatct ttttagaaaa
     1441 ttatcgggta ccttgttgtt tagtcaaaat tataaggggg agtaatagtg aggaacaaat
     1501 ttcataaact gttaagtatg tttctagtta tgcttattgt gatcaatcaa tttgggaaca
     1561 taacggttct agctgagtct ttagcagaac agccagagcg ttcgctgacg atcgaagttg
     1621 atcaagagga ggctgctttg ggtgagcagt tccagttgcg agtcagagga acaccagacc
     1681 tgctaaatgc attggtcatt gataagagtg aggatttgat tgaaggggaa gtgaagcaac
     1741 tcaatgatta cgaacgtctg attacttatt cttcccagca ttcaggtaaa tacgaattga
     1801 ccgcaagaag cggtgatatt caatcaaata cggtctcaat agaaatcagt gtaccagaag
     1861 aaagcacaag tgagtctttt aatgtaccag aagaaagcac aagtgaatct tttaatatac
     1921 cagaagaaag cttaagtgaa tcttttaatg aagccagatc atctgagcca tctataacgc
     1981 aggaatctca gattgaaaga caagaggagc tcttgaatta tgacatcggc atctctgctg
     2041 agcgccccac tttgccgagc aggcatacac aaacgttaaa gggatacatt caaggaggcg
     2101 ctggcgatat tcttgaagaa tatggggaga tggtcgtacg agttcctaga gcatcgattg
     2161 gcttaattga gttcattaga gtggaggtcg ctgatgattc acctttaaag ccactggcat
     2221 tttaccaaga aggcacagac gttgtttttc catttacctt tgattatgaa ggattaaatg
     2281 aagccgctca gtcgtataca ttcagtatca cgtttgaagt tgctttcttt caatcaggcg
     2341 gtactgagcc aaataatgac tttcaagtga gtcttactta tgctgagcga gcagcaaaca
     2401 cggaattttc aattacgcct attccttcat caagacccat tttttcgaaa ttatactatg
     2461 gctctagaga aatcgacagc caaggaaatc ttgggaatct gctgattgac gggcaagacg
     2521 tgaccaacag taataaattt attttgcagg taaattatga aggatcacga aaagacggtg
     2581 tagtggttac cgatgtacta ccgcaaggaa caactttggc accgaatccg actacatgga
     2641 gttccactgg aaatagagaa cctatttcag gtggggttcg gatcgcagaa gtaacgaatc
     2701 caggtgaagc aaacgttaga actcattatg tttccgagga atttgtggac aaagtatctt
     2761 ttaataagga aacgaataca cttgttgtgg actttggtaa tctagcagag ggtcaatcgt
     2821 attttattga atatgctctt gaagtgaccg atgtcgactt gttcaatgaa ggaagacaag
     2881 aggatgggtt ttctcggaat cgagcgagta tgacctacaa taatcaggta atgagccgag
     2941 actaccgctt gagaatccct gaaagtaatc ttccggcaat ctctttaaga aaaagtgtgg
     3001 acaaaaatgt tatcaatctc aatgaaaatg aactaacgta tactctgcat gtctcgatgt
     3061 tttctggaac aagaaatacg cttgttattg aagatgtttt ggaagaaggc atggaatttg
     3121 tgtcatttct atcagatatt ccctctttca ttgatgccga agtcaataat ggaatagttc
     3181 gtttcagtgc gaataatacg attggtggtc cgacaagttt taccctctct tttaaggtca
     3241 atgtagaggc gtatgcggtc ggcgatcaaa ttttaaactt tgcaacgctt gtgaatagtg
     3301 acaatcgatt gaatacgagt actgtaagaa caaggaaaat cgatgggcgt attaaagtaa
     3361 ttaaaactga tgagaatggc caaaggctgg caggagcaaa atttgaaatt ttatcaagcg
     3421 attcacaagt gatacaagaa ggtataacgg cagaaaacgg agaattttta agtgaaccgc
     3481 ttcctgtagg tagctatcaa gtccgagaaa cagaagcacc ggaagggtat cttttagatg
     3541 cgacggtaca tgatgttttt ataaatgaat actccataga agaagtgacg ttaaccattg
     3601 aaaacatcct tgatacgggt ggtgttgaac taattaaaca tgctgaaggc agtggtgaag
     3661 tccttcaagg agctgttttt gagttacaga accgagaagg agaaacgcta caaaccggtt
     3721 taactacagg agaagatgga aaactggcaa ttgacggttt agcaccagga gcatatcagc
     3781 tggtagaaac tcaagcgcca atagggtatg aattagatgc gacaccaatc gagtttgaga
     3841 tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaaccgactt acaccaggcg
     3901 gtgtcgtgtt gacaaagatc gatgaccaaa gcggggaaat cctgcaagga gctgtttttg
     3961 agttacagaa ccgagaagga gaaacgctac aaaccggtct aactacagga gaagatggaa
     4021 aactggcaat tgacggttta gcaccaggag catatcagct ggtagaaact caagcgccaa
     4081 cggggtatga attagatgcg acaccgatcg agtttgagat cgaaagaagt cagacagcgg
     4141 tggtagaatt gacgaaagca aaccgactta caccaggcgg tgtcgtgttg acaaagatcg
     4201 atgaccaaag cggggaagtc ctgcaaggag ctgtttttga gttacaagat gccaatggaa
     4261 ccgttctgca gtcaggtcta accacaggag cagatggaaa actggcgatt gacggcttag
     4321 caccaggagt gtatcagctg gtagaaaccc aagcgccaac ggggtatgaa ttggatgcga
     4381 caccaatcga gtttgagatc gaaagaagtc agacagcggt ggtagaattg acgaaagaaa
     4441 atcgacttac accaggcggt gtcgtgttga caaagatcga tgaccaaagc ggagaagtcc
     4501 tgcaaggagc tgtttttgag ttacaagatg ccaatggaac cgttctgcag tcaggtctaa
     4561 ccacaggagc agatggaaaa ctggcgatag atggcttagc accaggagca tatcagctgg
     4621 tagaaactca agcgccaacg gggtatgaat tggatgcgac accagtaact ttcaagatcg
     4681 aaaaagaaca agaagtggcg atctttttaa caaaagagaa tagaaaagtt gctgatagct
     4741 cagatataag aggaaacaat ccaacaagca gtggaaataa gcatcttcca aaaactggag
     4801 aaaccatcat tagccagttt atattgtcaa tattgggtgt gctacttgtt ttcatcagta
     4861 taaaatttag aaagaaacga aatatatgag ctaaaataaa aaattgaata agcatagttt
     4921 ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc gattttagaa ggggtataca
     4981 aacaaatatt agtatcggag tagagaaggt tgaacccaac cagtaaataa gtaaagacaa
     5041 ttttgtgctg cgttgagaag tgaaaaataa acctttgtag taatactagt taataaaaaa
     5101 tatacaaaaa cactgcgaaa atcattcttg agtttcgcag tgttttttgc ctaatgactc
     5161 aagtttattg ggtaatactt tgattttctt tctacaaata atcagcgatc agttgtaatt
     5221 ttttctctag ttttaatggc tctttgtcaa taaggagaaa aacaagtcgg gtcttttgtc
     5281 tcggaaagct tcaaccaact cgtgaataaa ggttacgcct ccttgaaagg cgtggaaaaa
     5341 ccaagtaatc gatcaatcac caatttcgca tttggtagaa catgtttggt gagctgaaag
     5401 taggtggcgt tcatgtctgt aactaagaat tctatttctt taggattagt gcagctaagg
     5461 aaatagtttg ttaatcgagg aattttttgt gtaggtaaat tttctcgatt aatttttctg
     5521 tctcgccatc cgcggaaata aaactcagtt tatcctcaac ggaaacgtga gaacgaaatt
     5581 cacaaccctc aacactcaag acagaattct tttggaagac tttggtaaat agcttctcaa
     5641 ctctttcaat gtgcgaataa cggttgtcaa agatacctaa caacttttcg aaataaaaaa
     5701 taacgacact ttttcagtca gtaaagaagt gattttatgt ctgacatgat ttgaaatcga
     5761 gtgccttgga cggataaaat aactttgagc gttccaatgg cttcgacagt ttttacaggt
     5821 atagcgctgc ttttttaggc gtataaccat tggcatatga ttatattatt caaagtggac
     5881 aatggtttcc ttcttcttgt ttttcaccac cattaatttc ctatttccat ctaccacagc
     5941 agaaccacaa tttctacaga cacatggagc agggaagaga actgcagcaa cgatcaatgt
     6001 ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc atcaaatttt tgtctattga
     6061 tctcagaatt tttttgatag aaccattcat aaagcatatc gttctctcag ttgtttattt
     6121 tgtggtgatt taatcataca agaaaacgat atgttttttt ataaaaacat gaataggaaa
     6181 aatagttgac aagaaaatga caacgccata tgatgttcca tagtatttac aacctgtcat
     6241 agatgactgt aaaaaataaa agtaaaaaga aaggacgaca acaggtggat ttttatcagt
     6301 ctctacaact agatccgttt attttaaaac aaaaaatacg agaagcagct tctaaaaaag
     6361 aaaagtgcat gtatatttgt tcattgtttt tacgatcatt attcatcgta ttgtttgcca
     6421 tttgttttat cattataatt acaacattat ttgaatcgaa gcataaacca tacgctgtgg
     6481 ttttattctg tatgttgatg agtattcgtt ttgttgactt tggttataag atttcccatt
     6541 cgatcatagg tttagcaatc gtcatgttct cactattagt agcaccgtat gtccagctga
     6601 tcaaatggtc agttatgggc atgttgattc attttatttt actaagcagc atattaatgg
     6661 caaccgctag tgatcccaaa atggggaatg cttctttgta cggcttttca tatctcttta
     6721 ttgtttattc gttacctaaa gattcgttga acaagaattt ctttacacaa acaagtagct
     6781 tattattttt gtttttttgt tggttttcag ttctccttta cagaaaacat cgagaaaaga
     6841 acaaagacaa aagcttgttt aaagagattt ttttgaaagg catgtattcg caggaaaaaa
     6901 tttggatgct catctatgca tttggcatta gtcttttgat cgttgcagga gagtatgtcc
     6961 cttttcagcg attgatgtgg gcagggtttg cattttcttc tattgtatct agctatggat
     7021 tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat cattggctca ttgattggat
     7081 gtgttttatt tattggtatc agccaattta ttccttttaa ctgggtcggt atattaggtg
     7141 gtctagcttt aggtgtttgt tctacctata gatataaaac agtttttaat tcttttggtg
     7201 ctttggcaat cactgccagt ttattcgggg ttccaggagc agtaacgatc cgcatttttg
     7261 agaatatcct tggggtatgt ttaggaatca tgtatattgg ggtgactgaa atacttatcc
     7321 gaaaaataag agaaaaacac ggtttgaatc attagatgag atcgaataaa aaataaggaa
     7381 aaagtgaaga tacacatgga tgataaattg ctatcattcc atgtgtatct ttttacggta
     7441 aatcagtgaa acgttcgact ttcattttta atttatactt tttacggagt tcgtttattt
     7501 gagccatcat ttcggatttg tgatgctttt cgattgcttc ttcatctttc caacggtcga
     7561 tcagtaaaag agattctgga tcttctagtg gaaagtaata gtcatatttc tcgtttcctt
     7621 cttctgcacg aatcgctttg acaatgtttc tttcgaccat ttcttctgca aattttctgg
     7681 cttgtccatt tgtacttgta tacaaaatat tcactgtgat actcactaaa tcccctcctg
     7741 aaaaaattct atcactagta tatcacggtg ccagaaatta ttattcaaga tatgatacac
     7801 attttttccg caatgctacg gaaatatata ttttgaatag aaaacgctta caagatatgc
     7861 ttggtttata aaaataaaag gagtgattga tttatgaaaa aagcattgat tatttgtgcg
     7921 gcaggaatgt catcctcgat gatggcctca aagacaacgg atttctttaa gcaaaaaaga
     7981 gaagaggtac tcgtagatgc agtttcagct acagaaggcg ataatatgat taagaatagt
     8041 gattttgatt tatttctgat cagcccgcaa acgacgatgt ttttggataa attcaaaaaa
     8101 ttaggagaat ctgttggaaa gcctgttgtt agtatccctt tccaagcgta tgtcccaatc
     8161 cctacaggca tccagaagtt agcagaggtg atcgaagaga acatatgatg gaataggagt
     8221 ggatgataat gaaacagaag gaaaaattgc tgttgagata tttgattgat cacaagaagg
     8281 agtatgtcac cagtcaacgt ttagcttctg aattgttatt atctgatcgg acaatccgta
     8341 attacttgca gcggatcaaa gagttagtag aaaaaaacgg aggtaagatc attgctaaac
     8401 caggttatgg ctaccaactg catattttac aaagactgac atttgacttg tttttgagtc
     8461 ggcaagaaat agtacctgga tatgctagag aaccgcaagg attttatgag tccgaagatc
     8521 gacaaaagta tatattgaac caattgcttt tggaagatgc cattcttttt atggatgatt
     8581 tggcagaaga gctttatatc agtcgttcca gtttgacaaa ggacatgcaa gaaatcaagg
     8641 agcgattgat tccatattca ctaaaaatcg tttctaagca tggtcaaggg acatggatcg
     8701 aaggggaaga aagaaatcga cggcatttta tcatggatac tttttttgga aaccattatg
     8761 gaaattcatt gaaagaatat ttagggcaaa gccgtttttt cccagacatc agttttgaag
     8821 agctagtcat tatcattctc gatgaaacaa gagaagctaa gttaaaagtt tcggatttta
     8881 tcatacagaa tctgacttta cacttggctt tggggatcaa acggctccga gaagggtttg
     8941 aaatcaagga attaggcatc gaacaagaag tttctaagcg agtagagtat caagtggcgc
     9001 agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc gtttcccgaa gaagaaattg
     9061 cttatttgac attacatctc atggctaagt cgaatcacaa agaaaatcaa aatgatcagg
     9121 aactatcaaa ggaattagcg aaagttctga aaagattgtc tcaggaattt catttttcat
     9181 tagtagagga tctccagctt ttcaatggac tactcgatca tttgtcaccg atgatgattc
     9241 gattgagccg aggtattcgc attgagaacc cattgactga ggagataaag aaagaaaatc
     9301 ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat gccggctttg aaaaactata
     9361 ccatcaatga agatgaatgg gcctatttgg ctttgcatct catggcggca ttggaaaaag
     9421 aaagagctgc gcataaacta catgcattga ttatctgtgc aacgggttac ggcagtgccc
     9481 aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa tattacagtg gtaagtgtaa
     9541 aaggctatta tgagatcaat gaaacaactt taaaaggaat cgacctgatt atttcttcta
     9601 ttgatctttc gacaatgttt ttcaaaattc cagttttaca tgtcagtatt tttttgaatg
     9661 aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc tataccctat cattcctcag
     9721 atagaccgat accagcattt tctctacagc aaaaaaagca gatttacacc gaacagttgt
     9781 ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa ggaagaaatt cttcatgatt
     9841 tgctgcaaaa gctgagcata aatgaagaag aaggatatat aagagaaatg cgaaagcaga
     9901 tccagcaacg tgaaaatatg ggacaaatca tattcagtga tacagtggca gtcccacatc
     9961 ccgctgttcc agttggaatc gaaacaaagg tagccgttgc attgattcct tctgggatgc
    10021 agtgggatca atatcaagaa attcattttg tttttttagt ttcgccttct tatgtcgaaa
    10081 atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt agttgaccag ctaaaagtac
    10141 agcaggaaat cttagctgaa cctatttttg agaatttcag cagtaaattt atcaaactga
    10201 tcgaataagg aggaaaacag atggctgaaa aaataaacag tgaaacacta caagtgactt
    10261 cttttgatat cattttccat agcggaaatg cacggacact gatacatgaa gcatttcgct
    10321 cgatgcgtaa agagatgttt gaagaagtag aagagaagtt gcgacaagca aatgaagaga
    10381 ttcttcatgc gcatcagtca caaactcaac ttctgaaaga atatgccagc gggacaaaaa
    10441 tagaaatgga aattatcatg gtccatgcac aagatcatct gatgacgacg atgacattat
    10501 tggaagttgc acaggaaatg aattatttgt atcaaaaaaa taaatagaaa ggaaggatca
    10561 ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa aaagcaactt agtttgaaaa
    10621 acatgtattt caatcgttat ctaatcgtac gttacctgac tgcctttttc tttttcatga
    10681 acatgcaatg gatgattctg cttctatcag ctaaaagttt aggaagtagt ttaccgatga
    10741 ttctgatttt agctattttg cctgcagtag gagaacaagt aaagctttat cgaaaacacc
    10801 aaacgaacgt tccatggaca aagtggtatt ttttgttcca gagtgtcttt aatttattag
    10861 tgattccagt gttgtttacc tcaggattta cattcttgta tccttttatg gcaaataaca
    10921 atggaggaca gttatttgtt ttcattcttg ttgtaagcgg tatttttgcc agtgcattta
    10981 tacagtatcg tttaaagaaa atttcactca accaagacca acagtatatc cgtattaaac
    11041 aatatgaaaa agcgttatat ttaggaaagg agaataacta atgtttgatt ttttgcagaa
    11101 atacctgatg ggacctatgg ggaaaattgc gcaacttaaa atcgttcggg ctgtcatggc
    11161 agcggggatg gcttctattc ctttcacgat tgtaggctcg atgtttttgg ttttaaatat
    11221 tttgccatta ccgttcccat ttttagaagg attttttaat gcaacttttt tcaaagttag
    11281 tgacttgtac atgatcgtca atacaatgac gatggggatt ttatctattt actttgccat
    11341 tgtgtttgct tatgaattaa cgtcaattga acgagatgaa caaaaattaa atgttaatcc
    11401 gctcacgggg gcattactat ctgtttttgc attctttatg tgtatccctg aattgatcat
    11461 gactgatggt aaaatcagct tgattgcttc tatcaatgag acagaaactg tagtaaatgg
    11521 tattaggatg ggctcttttg tcgaacgttt aggaacttca ggaatcttta cagctattat
    11581 tatgtcgatt attgcggtcg aattgtattg tatgtgtgtc aagagaaatt gggtcataaa
    11641 aatgccagat gttgttccgc caggagtttc tcgttctttc actgctctga tcccaacatt
    11701 tgtcattgcg tttgtggtga tgatcatcaa tggattatta gttgcactag gaacggatat
    11761 cttcaaaatg attgctatcc catttgggtt tgttacgcag ttgacgaata catggcttgg
    11821 tgtcatggtg atttatttct tgatccatgc attgtggatc gtagggatcc atggagcgaa
    11881 tatcatcact tctttcttga ccccgatcgt attagctaat atggcagcta acgcacaagg
    11941 cgcaaattat ccgttggcag gagaattcaa caattcttat gtaaccgttg gtggatcagg
    12001 tgcgacttta ggattgatca tctttattgc attcctcgcg aaatctgatc agctgaaagt
    12061 gttaggaaaa gcctcacttg taccaggatt attcaatatc aatgaaccaa tcattttcgg
    12121 gatgccaatc gtctacaatc cctatttagc ggttccgttt ttcttagctc cgatggcttc
    12181 tgctacactc gcttattttg ccattcggct ggaaatcgtc aaaccgatgc ttgcacagat
    12241 gccgtggcct tcaccagtcg ggatcggagc atttgttggt agtggtggtg attggaaagc
    12301 tgctgtgtta gcagtcattt gtgcaatcat cgcattcatt atctggttcc catttatcaa
    12361 attctatgat aataaattat taaatgaaga aagagaaaag gcagcagaat tagctagtga
    12421 aaagttaaaa tgatttctaa gaaaaaagga actgtgacaa aaaatcacag tccctttttt
    12481 tcttagaaat gggaaaccac tagtatataa ataccattat tcttcaatca accgcttcac
    12541 gattgcataa tccccttcat aaggttcatc tacaccattg attttttgat agggatccac
    12601 gccttttccc gcaataacga ctgaatcttg cggagcgctt tctgctaaag cgcgtcgaat
    12661 cgcttcagga cgatccattt cataatgaac gatcaaatct ggattagtaa ttgcttcatt
    12721 gatttcttcc gcgatttttt tcggatcttc aaatgctggg tcatctgccg tcaaaaaggc
    12781 gatgtcagct gtttctgata gcacttttcc aaaatcatgt cgacgggaga ttgctttgtt
    12841 tcctgggctg cctaatacaa caattacacg gccatctgga tgttcatttt tggcaaattt
    12901 cagcaatgtt ttcaagctta gataattatg tgcatagtcc acatagacat gcgctccatt
    12961 ggattggatc aattgatcca ttcggcctgg tacacgagct tcttttaatc cgtcttgtgc
    13021 atcttgactg gaggcacctg ttagtgctgc ggcaatgatc gcactggctg cattttcttg
    13081 gttgaatcct ccagccagca aaatatcata agcagtatcg cagatagcta agcgatcttc
    13141 atgagaagat aaagtaaaac cgtgagtacc tttatctgaa cggatcactt gataattgct
    13201 ttcttccgaa cgaccataag tgatcgttgg aatctggaat agatcggctg tttccctaag
    13261 taaatcaaaa tagtcacttt catgatttaa aacaactgtt tttgaattca agatcaattg
    13321 tcgcttgcaa tagaaatagt catcaaatgt cggatgttcg atcggactga tatggtcagg
    13381 cgagatattc aagaagattc cgacatctaa agtcaagccg tatacgcgtt gtgttttgta
    13441 agcttgtgaa gaaacttcca tcactagatg agtcatacca ttttcaactg cttctgacat
    13501 catacgatat aaatctaatg actcaggcgt tgtcaaatgg gatttgaaat atgttttgcc
    13561 atctaacgtt gtattcatag tagaaagcaa tgctgttttt ttatttgttg tgtgatccaa
    13621 gattgctttt gtaaagtacg cagcagttgt tttacctttt gttccagtga atccgatcac
    13681 tttcaattta ttttgcggat agtcgtaaaa agccatagca agaagcgcca ttgctttacg
    13741 gatatccgtt acgatgattc ctactgctga attttcgtat ggttgttctg atacataata
    13801 ttgaataccc tgttcgattg ctgaatccaa atattcttct ttgaaattca ttcctttaca
    13861 gaaaaacaaa gtagaagcat cagcttttct tgaatcataa cttaactgaa gaaattcagc
    13921 tttttctggt acatcaagat gccagcctct tgttgaaaca aattctttta atagattttc
    13981 ttttaatagt aaatgacgga tgtcttctaa agagaagctc attgattgat ccctcgcttt
    14041 attcaatttc ttggtttatt atagcacact ctttttaata tatcgtttgt ttccgctatg
    14101 acaaaatcca gctttattca aattttcttg acacagggga caagcggatg agtattttca
    14161 taaacaatga actgactgta atagatagga gaacgaatag tgtctccaac aatccaaaaa
    14221 cataataatt caaaaagacg ataagactat tggtaagtaa aagtccttgc ggcattggaa
    14281 gccgaaaaaa tttgtggaaa ataatcggcg gtatcgttat accgccattt gaggccccga
    14341 ctgtgtacaa tgtgtaaacc ccaagactga acaaaagact tcctgaaatc agtgaaaaga
    14401 aatgtgagga gatcatttcc acttttggaa caatctctaa aacaacagga aaaagcagac
    14461 ttcccgctac agtcttaaaa aatactgatt ttcctaatag gacagcggct agaagcagca
    14521 tcataagatt gatgctccaa acagtaactg agcgttggat agaaaaggcc gtttcaacta
    14581 aatgtccgat ggcgcccact ccggcggaag ctatatcgtg agggagcaag aaccaattca
    14641 atccaactgt gataaagaaa agacctaaaa atataacggg tagttttttt atttcttgtg
    14701 tcattgatat ctcctataaa tgttagccat aaaaggctgt atatttcttt actttaccaa
    14761 aaaaacgaaa ccgaattcaa aaaaacaatt gttcttataa aagttatata aaatcgattg
    14821 ccagtttctg ttttaccaat taaaatgata gtaatgtttt gtagaaagtt ggaatctgac
    14881 cgatggataa tcaaagaaaa ccagcattag atcaattaac accgcaagaa aaaatggcac
    14941 gaggctctgc ttggatgacc gccagtaata tcatttcaag attgctaggc gctgtctata
    15001 tcattccctg gtatgcttgg atgggcgaaa atgctaaggc tgccaatgga ttattcaata
    15061 tgggatacaa tatatatgca ttgtttctac tgatctctac agcaggaata cctgctgcga
    15121 ttgcaaaaca aactgcacgc tataattcat tgaatgaata tggcacttca cgacgattat
    15181 ttatccgtgc attacagatg atgggcgggt taggcctgtt gtttgctttg ttcatgtaca
    15241 tcgcctctcc ttggctagct catgcttctg gtggcggaga agaactgatt ccaacgatgc
    15301 gttctttgag tatcgctgta ttggtttttc cttgtatgag tgtgatacga gggtatttgc
    15361 aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta
    15421 ttttttatat gctattggct acattcatca tcatgaaagt catggatggt gactatgtga
    15481 cggcggtcac gcaatcaact ttcgcggcat ttatcggtat gctagcaagt attcttgtcc
    15541 ttttgtattt tttgaagaaa catcaagcgt atacatcggc gtttattcac tacagcgaaa
    15601 acaaaataaa tattgcaacg aaggaattgt tgttagatac cgttaaagaa gcgattccgt
    15661 ttattattgt gggttcaggg gtaacgatct tcaaattagt cgatcagttc acttttatga
    15721 agatcatgtc tgcatccacc gagtattcca atgcacagtt actggatctt ttttcgattt
    15781 ttagtgccaa tccagataag ttgacgatgg tagttattgc attggcaaca tcgatcgcgt
    15841 ctactgggtt accgttgatt acagaagctg ttacgttgaa agatcgagta ggtctggcaa
    15901 aattgacaag cggaaattta cagttgtttt cattttttat gtttccggcg acatttggtg
    15961 ttatgttact tgcctatccg ctaaatacat tattttatac accggattct ctaggaagta
    16021 atgtactgat ccaagcaagt tttgtaggac tcttcctagg actatatatg ctagtttcca
    16081 acatgctgca aggaatgttt gaaaacaagg cggcaattaa ttatttggtt attgggtttc
    16141 tagtgaaact ccttcttcaa tacccagcca ttcgtttatt tgaagtatac ggccctttat
    16201 tggcgacaat gatcggattt gctgtgtctt gtaccttgat cttaaagaga atccatgaag
    16261 tagcccgctt taaaccaagt tttgtctggc gcaggacatt gctgatcttt attcttacct
    16321 tgattatgct tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag
    16381 atagcaaatt ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt
    16441 atggttattt agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc
    16501 gtttacgaca taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt
    16561 tgaaatgggg gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc
    16621 gcaaagaagt caaaggatta atcaaaaaag gaacgatcga agtcaatgga caagtggtga
    16681 aaagcgataa agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca
    16741 ttacgtatca aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta
    16801 ctcaggataa tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag
    16861 atctttttcc agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg
    16921 acggggtgtt tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag
    16981 ctgaggttag cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta
    17041 ttgatggcga tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga
    17101 cagaaacttc aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa
    17161 tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt
    17221 ggctgcctaa agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac
    17281 aagtgagaga aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc
    17341 atctgaaaag cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt
    17401 atttccgtag aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac
    17461 ttttgttaca gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc
    17521 cctaatactc aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct
    17581 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc
    17641 tctaatactc aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc
    17701 ctatactttg tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc
    17761 gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg
    17821 cgttataaag cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt
    17881 tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg
    17941 ttcttggcta gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt
    18001 tcttcggatc aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc
    18061 cttttgaatc tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa
    18121 cgactcgtta aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt
    18181 atcagttact ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc
    18241 actggacatg gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc
    18301 ttggtgttca ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct
    18361 gttatgggaa tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg
    18421 cagcagatca aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt
    18481 ttagccgtag gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat
    18541 tccggtgcat tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca
    18601 tccacaggag attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt
    18661 gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc
    18721 taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata
    18781 agacggctaa ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc
    18841 catttatatt tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt
    18901 acagtttttg gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc
    18961 aggataaaat tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga
    19021 tcaatagcac ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc
    19081 gtcgtttttt taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca
    19141 attatcttgt tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc
    19201 caatggctgt cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct
    19261 ctgattcgat tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc
    19321 agatcgtttt accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg
    19381 gttactggaa tgactggttc aatgctttac tatacatcca agatgataag ctagttccac
    19441 tacagtatct tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag
    19501 caggaagcca attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga
    19561 tcgtagtcat ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg
    19621 tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa
    19681 ttaggaggaa gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg
    19741 ttgttgggaa cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc
    19801 aattcggaca ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg
    19861 atggaagtag caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac
    19921 attggctggg gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat
    19981 gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg
    20041 actgaattag cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt
    20101 aaaggaaatc tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct
    20161 caacaagtac ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga
    20221 atccaatctt acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac
    20281 acggcagctt ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta
    20341 gggaatacgt taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa
    20401 tacgataatg aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga
    20461 ctcattccaa gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg
    20521 ctaatgcgtg gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca
    20581 gcaaaacaag aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca
    20641 caaatggcaa attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt
    20701 ctagggctgc ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga
    20761 gaagcatggg aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag
    20821 ccaaatacac atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag
    20881 tctattacgg atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca
    20941 ccgattttag gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct
    21001 aatgtcatga atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca
    21061 ttaccgaaat tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg
    21121 caaaaacaat atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga
    21181 caaaagacgt gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg
    21241 tattaagcta aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac
    21301 tcgaaactaa gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc
    21361 ggtattcgct cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata
    21421 atcaggtcaa ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa
    21481 tgtttggagg agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg
    21541 tcattataaa actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg
    21601 gattaggtgc ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata
    21661 aagaaatcac aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc
    21721 tgctatttag tttattttta tctggtactg ctttattgag ttacaattta tatttatctg
    21781 ttcaaatcaa agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct
    21841 gcctatttgc tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc
    21901 ctaatctgct aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa
    21961 taattattgg tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt
    22021 ttggtgtcat tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag
    22081 aattagataa acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc
    22141 ttactaaatc gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct
    22201 accgttattt ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca
    22261 agtgatgccg ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc
    22321 atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca
    22381 aaacccatag atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta
    22441 gtttctttcc caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata
    22501 tatatcgtgt tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg
    22561 gaaataaaaa aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca
    22621 caaggaggag ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg
    22681 attttagaaa atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca
    22741 aatcgactat acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa
    22801 atgatcatca cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa
    22861 aacaattttg ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat
    22921 aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt
    22981 attttagaca atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa
    23041 gtatctatcg tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc
    23101 gacataacaa acacggaata tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg
    23161 gatagcatca atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca
    23221 catatgcggg cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat
    23281 atccgaatgt atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt
    23341 tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg
    23401 gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat
    23461 cattttatga tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca
    23521 ttagttgaaa attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt
    23581 gtcaaagtct tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg
    23641 atgactgaaa aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaactt
    23701 cacggttcta tcggtctgca aaacgtgaat gaacgtttac gtgccagctt cggcagcagt
    23761 tattatatgt cgcttgagaa taatgagaca ggtggactga ccgtttctat tacattcaag
    23821 gagggatgag cgtgtatcaa gtgttattgg tagatgatga atacatgatt ttgaatgggt
    23881 taaaaaagat aattgattgg caaagtttag gctttcaaat agtagcaaca gcagagaatg
    23941 ccaaagaagg actagctgta cttgaacagc gacagattga tttagtagta acggatgtga
    24001 caatgccaga aataaatgga ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt
    24061 ttgaatttat gattttatca ggataccaag aattcgatta tttgaaagga ggtatgcaat
    24121 taggcgccgt gaattatttg atgaaacccg tgaataagtt cgaattggtt gaaagcctca
    24181 agaaaatcaa aacacggtta gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc
    24241 aagaactgtt attcagtcaa tggttgaatg aagaattgga tgaaataagt gaagaagaaa
    24301 taataggaca aataagtgag aagcagcata ggatagtatt gattcaactg gcaagaactc
    24361 atgatactct tatcaataga tggttgcaag aacggcaaca aatcttttac tatcaacgaa
    24421 attacggaga ccgtgtattg atcacgttgt tgttggaggc agatgaagtt gatggcttct
    24481 gttgttttgt tcaagaaaac tttcctgatc aggaatggct gatcagtatc ggggaagaga
    24541 cagattcttt ggacaaaatc cctgaaacat tccaacaagc gaaagacaat ctacagttgc
    24601 atcaatttta tggagataaa gacgagcatg ttttttacgc agtacaagca gaaacaaaag
    24661 atcaaaccat cgatttttca agtttcagac gcgtgctgca aaataaacaa ttggaagtcg
    24721 ctcaaaaaat gatcactgat ttttttgaac agtttcagtt agcagccatg atgccggaag
    24781 atatacgata ttccagtttt ttactgttta tggagattca acgggaattg attgatctgg
    24841 aagatgagga atatttacaa ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac
    24901 ttcagcagct acttctgtca ttcatcaaaa agcatcagag acagaaaaag tacagtgaca
    24961 atgtggaaaa agtaatcgta atcctgcacc agcattatca agaaccactt acactcaaag
    25021 aagtgtcaga aaacctgcat ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa
    25081 cgaaaaaaag tttttcagct tatttgaacc atttacgtat ggaaaaagcg aaacaactgc
    25141 ttttgcacag taatcaaaac atcaatgaga tagcaagtga gattggctac aacaacacca
    25201 cgtatttctc taaattattc aagaaaatcg tcggcagatc ccctaaagag taccgagaaa
    25261 acagtggaga atcataaaag aaaaagccaa tgtaatgaaa taacttataa aaaattgtct
    25321 agaaatctaa ttttgggctg ttcttattac ttgaggcaag gcagcttttt catgacctct
    25381 cgataaacgg agttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
    25441 gaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacgacct
    25501 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa tggcaaaata
    25561 tgaggaacta ttttgccatt tgtctctcta atactcaggt cagaacgctg atttcacaac
    25621 ctctcaataa acggtgttca aaagcttgca tctgcggtaa taagaccaac caaacgaaaa
    25681 atatgaggat ctatttttgc ttggctgttc ttattacttg atgcaggaca gctttttcac
    25741 aacctcttgt taattatagt ttgataagga aaaaggcaaa tcttttgctt gatatttctt
    25801 agggtgaggg atgatcccta attggtaaga gatcgtgccg cctttcagca aatcttgatg
    25861 ggtaaaatat aatttcgtat attctttgtc catccgtttg attttatgga taaattgttg
    25921 ctgcggccga ttaggcgttg cttcaatcgt caattccttt ccatttgaca aaagcaaaac
    25981 agccttgtca attaatggca taccgataac atattcaccg gttccaggcg tgacaggata
    26041 aaatcccaga ctattgaaga tataccaagc ggccatactt ccattgtcct catctccagg
    26101 gtaacccatt ggagaatcgt cgaaggtctg agtcatcaac tgtttaagca gtggctgtgc
    26161 catttcaggt tgtcctatat aactgaacaa gtaaggatag tgaaaacttg gctggtttga
    26221 aatagctaat tgtccaaatt ctatcgcggc catctcactc atttcgtgga tctcaaaacc
    26281 atatcctcct gggtcaaacg ttgggggctg attgcacagc tcaatgagtt tttctttata
    26341 tctctcactg ccaccatatt ttccgatcag tcctgaaaaa tcgtgataaa cagcaaagct
    26401 gctttgccaa gcacttcctt ctgcataatc ttgtccccaa cgattagctg aaaatggttc
    26461 tctgaacttg ccatctttat cttttgaacg cataaatcct gactcatagt caaagatatt
    26521 ttgatagttc aatgattgct gctgatattt cttcgtcatt tcctcttctt ctagtacctt
    26581 ggcaacttgg ctaatacaaa aatcgctata agcatagtct aatgtgtgat tgactgattc
    26641 gtggtagtga ttaggaacat agccatatgt caagtaatct tctgttcctt ggcgtccata
    26701 atttggatgt ttgctttgaa ccgtagcacc tttttccatt gccgttagaa attcaggcat
    26761 caagtctcgt cggatatctt ttacagcagc atctgcaatg accgcatcga tcaatgtccc
    26821 aggcataagt ccacgttcat caggagacag ccattttggc aaatagccac tttctctata
    26881 gctgttcaaa aaaccttcca gcatttcttc gtatttttct acagcaatca aagaataaag
    26941 aggataaacc gttttatacg tatcccaaaa cccattattt gtatagagaa caccaggttt
    27001 tatagatcga ctagtcgtat catagtggat tttttgccca ctaggatcaa tttcataaaa
    27061 tgtttgtgga aataaaaaca cccgatataa attatgataa aacgtccgca attgttgttg
    27121 atcatggtgt gtgagctgta tacgatcaaa ataggactgc cattgttctt gactgtgagc
    27181 aaggtaagtc tctttgcttt ggttttgttc tagttccagg ttataagctg cttgttccga
    27241 gctgataaaa gatgtcccaa atcggatagt ttgttttttt gtctgttcaa aatacaacgg
    27301 gatcgagtcg ctatttttgt tggaaacagc taactcatca attgttaaag gatattcaaa
    27361 tctcaatata aaataaaaag aaaaatcagg atcttctgaa ccagcaaaat gtgtgatttt
    27421 tccttccact gtgtaaggat ctataacaaa gagctgatgg cgtcccggaa aggataaaag
    27481 taaaccgctg tcatttttgc tgtaatcaat agataagatt ccgccataca tgctaggtat
    27541 caatgtagaa cagatgccat aacgtaattg acagatcgac agatgagtcg ggcaaaatat
    27601 actttcttct gggcggtaag aactttgagc atggaaaagg gtgttctcct gtagttttcc
    27661 attgatcggc gtaaacagca tatgactgaa atcacccatc caaggacttg gttgatgggt
    27721 aagacggtag ccttgaaaaa cgcggtcatc aggatgaaac caccagctgc cgttttgatc
    27781 cgtagtttgc ggtgcaaaat aattcatccc aaaaggaacg ccagtataag gctggcagtt
    27841 cccattagag aaatttggct gattcgctgt tgcatgtcta gtatctattt gaccgatatt
    27901 cattttattt ctcctttgtt gattgtcttt tgatgaatga aactggaaat ttactatcgg
    27961 atggtcgatc ttttgtctct aaccaagtga ccaaagcatc tcccgcaagc ttacctaact
    28021 gttcaaaatc ttgtgcgatg gttgtcagtg gcgggtcaac aagagctgct gcttggatat
    28081 catcaaatcc gataattgaa aaatcttcag ggatatgata accatgctgt ctcaagagat
    28141 tcatggtctg gatagcgaca acatcgtttt cacaaacaaa agcagttact tcatacttag
    28201 tgatataaga aagcaattca tctaatgtcg cgcttttgtc atccagtgaa gtgtggaaag
    28261 tcaaaccagc tttgtccaat gcttcaaggt aaccaaggta acgctgtcga acggattgtg
    28321 gatgagtctg cgtaccaaac aaataagcga tttttgaatg tccctgttcg atcaaataag
    28381 aggcagcaag cgcaccgcct tggaaattat cggaaagaac ggtaggaaaa ggtaactcat
    28441 agattttttt atctaatatg acgacaggga attctttcaa tgaaagctca aaaagtaagt
    28501 caagatgttg ttctgtatca agagcataat aaatcaatcc gtcaaattca ttgattagct
    28561 cttttgctgt tttattttgt agaaagtcaa gcgtcgtcat catgacatca atctgtttat
    28621 cttgcatcac agggttcaat cccttgctaa aatcaccaac agacaaatca gcgacatatg
    28681 gaagaagaaa cagaatccgt cgtgtttttg taggagaagc aagaatctgt tccggagaag
    28741 cggatttaac gaaactgcct ttcccgcgaa cgcgataaat aagtcctaat tgctctaatt
    28801 cagtcaatgc acgtttggaa gtgattctgc ttactttata ggtacgtgaa agttcttttt
    28861 cagttggtag ttggctgtct acaggtaatt caccagttaa aatagccgtt tgcaaatcat
    28921 taaatattgt ctggtacaat ggttgattca tataaacacc tcttaatgat atatcatatt
    28981 caaataatac cataatggat gaaaaagtaa ataaaaagat attttttgaa agcgtagaca
    29041 taattggctg tatgaagtat aatctttaaa gatatatcaa atagaaagaa ggttttatta
    29101 gtggcttata aagatatacc aaaatcggtg cagaaattta tggaagatat cacacaaaaa
    29161 tgtggggata tccatgcgga ctgggcaaag aattttacag cagcatttgc caatactctg
    29221 ttgacaacag tcaaaaagca aaatgacgga accacctttt tattgacggg agacattcca
    29281 gcgatgtggt taagagactc tactgcacaa gttcgtcctt atttagtgat tgcaaaagaa
    29341 gatgaggaat tagctgacat gatcagcggg ttagtcaaaa agcaattttt ttatatcaat
    29401 atcgatccat atgcgaatgc cttcaacgaa gaagctaacg gtgctggcca ccaaacagat
    29461 catacaaaaa tgaacgattg gatctgggaa agaaaatatg aaattgattc tttatgctac
    29521 ccaatacagt tggcctatct tctttacaaa aatacaggaa aaactgatca attcaatcaa
    29581 tcttttattg aaggtatcaa aaaaatcttg gacgtgttca aaacagagca agatcatgaa
    29641 cactctcctt atacatttgt acgagacacg acaagaaaag aagatacgct gatcaatgac
    29701 gggcgaggtg caaaaacagc ttttacagga atgacatggt ctggtttccg tcctagcgat
    29761 gatacttgcc gttatggata tctggtacca tccaatatgt ttgcggtggt cgtgttaggt
    29821 tatgtggaag aaatttttca aacgatactc gatgataaag aaattgtaca gcaaacacag
    29881 aaattgaaga aagagatcca agaggggatt cagaatcacg gacgaacggt taatcaaaaa
    29941 gggcaaacga tttatgcata tgaagtagat ggaatgggaa acgccacagt catggatgac
    30001 agtaatgtgc ccaatctgat cgccgcacct tacttgggat attgttctga acaagaggaa
    30061 gattatctgc aaacaagaag aacattgttg agtaaagaaa atccatattt ctatgaagga
    30121 aagtacgcaa aaggaattgg atcctcccat acgccagaaa aatacgtttg gccgattgca
    30181 ttagctatgg aaggaatgac aacgtcagac aaatctgaaa aagagcggat attgaattta
    30241 cttgtagcaa ctgacgcagg aacacacctt atgcacgaag gattcgatgt gaacaatcct
    30301 gaaaattata cgagagaatg gttttcttgg gcaaatatga tgttttgcga attagtcatg
    30361 gattactttg atattagagt agaaaagtag gaggtatcgt aaatgaaaaa gaaagtatat
    30421 atcatttctc atagccactg ggatcgtgaa tggtacatgg cttacgaaca gcatcacatg
    30481 cgactgattg aattgataga cgatctacta gagctatttg aagttgatcc gtccttcaac
    30541 agttttcatt tggatgggca aacgattatt ttagatgact atcttcaagt aaggccagaa
    30601 aaaagacagg caatccaaca agcaatcaat gaaggaaaat tgagaattgg accattttat
    30661 atcttgcaag atgattttct tatcagctcg gaatcgaacg tgagaaatat gctgattggc
    30721 atggaagaaa gccgtaaatg gggaacccct gtcatgttag gctattttcc cgatacattt
    30781 ggaaatatgg gacagacgcc tcaattaatg aaacaagctg gtatttcagc agcagcgttt
    30841 ggccgtggtg taaaaccaat tggctttgat aatcaagttt tggaagcaga aaactattct
    30901 tcccaatact cagagatgtg gtggaaagga cctgatcaaa cagcaatctt tggtctgcta
    30961 tttgccaatt ggtatagcaa cgggaatgag attccagtag aaaaagaagc agctcttgcc
    31021 ttttggaagc aaaaattagc ggatgcagaa caatatgcct ctacgaacca cttattgatg
    31081 atgaatggtg ttgatcatca accggtacaa aaagatatat caaaagcaat ccatctcgca
    31141 aatgaactgt ttccagatta cgagtttatt cattctaatt ttactgacta tctggaagca
    31201 gttcaaaaag atgtgccaga agacttaggc tcagtagaag gagaattgac aagtcaagaa
    31261 acggatggct ggtatacttt agccaataca gcatctgctc gggtttactt aaaacaatgg
    31321 aataccaagg tccagcgaca gctagaaaat attacagaac cacttgcgac gatggcttat
    31381 gaagttagtg gaaactatcc ccatgatcaa ttagattatg cttggaagac attaatgcaa
    31441 aatcatcctc acgacagtat ttgcggttgt tcagtcgatt ctgtccatcg ggaaatgata
    31501 cctcgttttg aaaaagcaga tgaagttgga aagtatcttg cacaagattc cttagaacag
    31561 ctgaccgcag caatcgatac gaccggattc ccaaaagaca gctttccatt tgtcattgta
    31621 aatactgcag gaatggataa aactggtgaa gcagaaataa ccatcgaatt agaaagaaaa
    31681 agattcgctg aaggaatacc tgaacaacta tatcaagagt tagaaaattt gcctaaacgt
    31741 aagtatcatg tagaaacaaa aagtggggcc actataccag ctattttatc tgaagaaact
    31801 gtccaatttg gttatgattt gccgaaagac cgtttccgtg tgccatatat ggcaagaatg
    31861 atcaaagtga ctttaccatt agaaaacatg ccagcatttt cttgggaaac attcgcgctt
    31921 gtagaagggg aagcgaaagc agaagaaaag gaaacaatga ttcatcagag tggacgaatc
    31981 atcgaaaacg gccctctaca tttgacgatt gaaaaaaatg gtaccattac aatggaagat
    32041 cgcaaaaaca aacgaaagtt gaataatctg catatttttg aagatatcgg cgatatagga
    32101 aatgagtata tctttaaaca acctttctgt gatcagccaa tcctatccag taacaaagaa
    32161 aactctgaag taaaagtatt agtagataca ccggaaatag caaaaataag tattctacag
    32221 gaaatggaaa tccctgtttc tgcagacgag cgtttagaaa aagaacagca gatggttgtg
    32281 gaatttcgtt atcgaaaagc agaacgatcc aaagaaaaac gaatccttca aataaaaaca
    32341 atcatgacaa tcagaaaaga tagtaaaaaa atcgactttg aaacgactct cgacaatcag
    32401 atgaaagatc atcgcttacg tgtcttgttt cctacaaagt tacatgtgga acatcacgaa
    32461 gcagatagta tttttgaagt ggtgaagcgc ccaaaccatg tgtctaaaag ttgggaaaac
    32521 cccacgaatc cgcaacatca gcaagcattt gtcaatattc atgatgaaga gtacggtgta
    32581 acagtcggaa actttggttt gaatgaatat gaagtaacag aagacggaca aatcgcagtg
    32641 acgctacttc gcagtgtggg agaactaggc gattggggat attttccaac accagaagca
    32701 cagtgtctag gtgagcatcg gttcaattac agcattgaac tacatggacc agaagaaaaa
    32761 ttctctactt atttgcatgc atacgcagca cagattcctt tttccactca acaaatcaaa
    32821 catcatgaag gaacattgat ttcaaaacaa caatatttaa cgataaaaag tgaaacgttt
    32881 gcaattacag ctttgaaacg cagcaagttt agtgataaag tagtagtaag aggtttcaat
    32941 atgtctagtc atttagaaaa attggaaata actaaagaca atggaaaaac tgtcatattg
    33001 aatctgcttg aagaaccaac gaagcaagcg gttgtcccaa taatccaacc ttatgagatt
    33061 cggacaatcg gattcgagga ggaaaacgaa tgtatggcgg aatagaagca ggcggaacaa
    33121 aatttgtgtg tgcggtttca gatggtggag aaattttaga aaaattaagt atccctacca
    33181 caacaccaga agaaacattg gatcaggtat ttgaattttt tgatcgttat gaattgaagg
    33241 gaatcgggat tggctcattc ggcccgattg gtatcgacaa aacaaaagat aattacggat
    33301 acgttttggc aactcccaaa aaagggtggg cacaattcga ttttcttggc acaatcaagg
    33361 aacgctacaa tattcctgct gcttggacaa cggatgtcaa tgctgctgca tatggggaat
    33421 tgaagcaagg agcagcacaa ggaaaaaata gttgtattta ccttaccgtg ggaacaggaa
    33481 tcggtgcagg tgtcgtgatc aatgaagaaa ttttttcagg gatcgcacat cctgaaatgg
    33541 gacatatttg ggtgaaacgc catccagatg accattatga aggtacttgt ccttatcata
    33601 aagattgttt agaaggatta gcagcaggac cgtctattga ggcacgcaca gggatcaaag
    33661 ggcaagatct gccgcaagat catcctgttt gggctattca ggcttattat attgctcaag
    33721 cactgatcaa ctatacgttg acgttagcac ctgaaaagat tattttaggc ggtggggtaa
    33781 tgaatcagga tcatctcctt caaaaaatcc gtcagcaatt tgttgaattg atgggtggtt
    33841 atatggaaac acctcaagta agtgaatata tcgttcgttg gggactgccg aatgaaagtg
    33901 ggattatcgg tagtttgctg ttagcagaac acgaacatca aaagtaagca gacaaattac
    33961 acaaaaagaa cagagtctta tcattatctg ctgataagac tctgttcttt tttatttcta
    34021 ttcccataat ccaggtaaag ctttaccgac tattttttct tgagtaaatg gatggatcat
    34081 gtgcagttct aatgcatgga gcatcagtcg ttcagctgtt ctattttggt agagagggtc
    34141 tccaacgatc ggatgtccct tatgagctaa atgaactctg atctggtgtg tacgtccggt
    34201 ttccaaaaca caatagactt gtgtttgttc agaaagtact ttgttgacag tgacatgtgt
    34261 caaggcgctc tgaccatttt tatcatctac gacccttttt ctccgatcat ggcgatctcg
    34321 tccaatttta ttggtgatgg tcatatcttt ggcaatttct ccccatacga tggcttgata
    34381 tctccgatag atcatttttt gttccaacat tcttccaagt atcggcaaga caaatggatt
    34441 ttttgcaaat aaaacggcac cgcttgtttc tttgtccaag cggtggacaa cgtaaggtcg
    34501 ttgctgttta gtgtcgagat aagcagctaa atgattgagt aaggtactgt tttctgtcgg
    34561 ttcatttgga tgagtcttca ttcctgcagg cttatccact ataatcaaat gctcatcttc
    34621 atataaaaca cggatgtgct tcgcatctcc taattgaact tcttgataat tataatctgt
    34681 ttcttcgaat aacaatgtga tctggtcatt gccctttact tcatgatgga acaaagcagg
    34741 ttgttgattg atccaaacgt ttttacgtat acgcaaaaag tgtcgaacct ttctaggaac
    34801 taaccattcc ttttcaagca agtcgcgaat tgttgtttca ggatggttca taggtaaagt
    34861 gatcgtgatt ttcacaagtg tactcctttc agtgcaatcg cagactaatt gtaccatttt
    34921 taaaacatat ttaagaaaaa acttacattt ttctgttcat tcttatgtta ctctacttgc
    34981 actaacgtaa aaaaacatat tgaactacaa cttaagcagg aagaaaacac tctttcacta
    35041 tggtaaaata agacgattga atcaaataga caggagaaga agcaaatgga ttttcaaagc
    35101 attttagaaa agataaaagc tgctttgatc agtttttggc gttggatcag accatatctc
    35161 ggacaattcc atcgatggcg aaagcgcatc tggaaaaaat atcatgtgaa caaattgatt
    35221 ttgctgattg gtttgatctg tgtcttagtg acaagtatct acctctttat tttagcaaaa
    35281 caagcaaatg tcgaaacgtt aaaatcagga ttaagccaat cgacagtcat ttatgataag
    35341 aaaaacgaag aagctggcac actgtacgcc caaaaaggaa catatgtaga attaaacgcg
    35401 atttctccct atatccaaga tgcagtaatc tcgacagaag ataggaattt ttacgagcat
    35461 cacgggtttg atatcaaagg gattgcccgt gcagcagtcc gaatggtctt gtctggcgga
    35521 acatcagggg gcggaggaag tacgatcact cagcagcttg ccaaaaatgc gtatctgaca
    35581 ttagaccaaa cgtttgatcg aaaagcgaag gaattatttt tggcaatcga gatcgaaaag
    35641 aagtacagca aagaagaaat cttgaccatg tatcttaaca atgcctattt tggcaacggg
    35701 gtatggggtg tccaagacgc ttccagaaag tattttggag tagatgcaac gaatgttacg
    35761 ctgtctgagg ctgctacttt ggcaggaatg ctgaaaggtc ctggtattta taatccgatc
    35821 gatcacccaa aaaatgcgaa tgatcggaaa aacacagtac taagtgtaat ggaagaaaac
    35881 ggaaaaatca caaaagaaca agaacaagca gaaagccaga cagatatcag cgcgtatctt
    35941 aatgacacat acgataattc agattctggt tatcgttatc cttattattt tgatgcagtg
    36001 atcgatgaag cagttaacga gtatggtctt gatgaagaag atgtcatgaa taaaggctat
    36061 aaaatctata cagctttaga tcaggattat caaaagcaat tagaagctac ttatcaaaac
    36121 agtgcattat tcccagcaaa tgcttcagat ggtgccatgg tccaatctgc ttcagtagct
    36181 ttagatccga atacaggtgg tgtacaagca ctagtaggac gaagaggaga tcatgtcttt
    36241 agaggattca gctttgctac acagatgcgt cgttcaccag gatcaacgat caaaccatta
    36301 agcgtatata caccagcact ggaagctgga tacaagcctt ccagtatctt gaaagacgaa
    36361 ccgcaaagtt attacgatgc gcataatttt gacgggacat accaaggaga agtaccgatg
    36421 tatcaagctg ttgcacaaag tttgaatttg cctactgtat ggcttttgca tgagatcggt
    36481 ttacaaaaag gatatgataa agcagaagaa ttcggcctgc ctttagcaaa atctgataag
    36541 tattatggct tagcccttgg tggactggaa aaaggagtat ctccattgat catggcgggt
    36601 gcttacagtg catttgccaa tgatggtcag atgtatacgc cgcatcttat cacgaaaatc
    36661 gttgattcga ctggagcggt gatcgtcgat aacacaaaca aaaaaccaaa acaggtcata
    36721 tcaaaggaaa cagccgatga aatgaccagc atgcttcttg gtacattttc aaatggaaca
    36781 gcggctgctg cgaatcctgc aggttatacg atagcaggga aaacaggaac cacagaaact
    36841 aatttcgatg ccacaaaagc aaatgaccaa tggatgatcg gttacacgcc agacgtagtg
    36901 atttcgacat ggatgggtta cgaagaatcc agcaagctgc actatttgga agggacaagt
    36961 ggaaatgttg tcgggaaagt cttcaaatca gcggcagaag gcatccttcc gtatacttca
    37021 aaaacaggat ttagtgtagc agatgcctat gccacaggag gacaagtggt agctgcagat
    37081 caagttggaa acacaggaaa ttctaatgga gagtctggaa attggcaaga taacttgaat
    37141 caatatggtg aacaagcaaa agaagggctg aaaaacttcg gcgatatggt gaaagaaggc
    37201 gttcagggaa ttggtgaagc ggctaaagac ctctggcgga aatatcaagg agagtagccc
    37261 ctttgaaaac gaataacccc atgttacaat agagagtaac tactaaaaga aaagagggca
    37321 taacatgtca aacatctacg atagcgcgaa tcaaatcgaa cgtgagatcc gtgaattacc
    37381 agaattcaaa gcgttgaaag aagcgtacga gaaagtacaa gcaaacgaag aagcacacaa
    37441 actgttcaaa gacttccaag cgatgcaaat cgaattacaa gaaaaacaaa tgagtggaca
    37501 agagttcagc gacgaagatg caatgaaagc acaagaaatg gctgtgaaaa tccaatcaga
    37561 agaagtaatc aatgacttga tgcaaaaaga gcaaggcttc agtacaatca tcaatgactt
    37621 gaaccgtatc atcatgacac cagttcgcga cctttacagc gaataaaact attttatgaa
    37681 acaacagagg gtgtgaaaga gacgttcggc cttgagcgat gacaaaattg cttcttattg
    37741 ccgataggct gtctcttgaa caccatttac caagaggtcg tgagcctacc gtttaactct
    37801 gagttacaag gaacaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt
    37861 gaccgaggag ttggcaggag aacaccgttt attcagaaat aggagctacg acaaaagt
//
LOCUS       DACCIQ010000020        37735 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCIQ010000020 DACCIQ010000000
VERSION     DACCIQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.020"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(334..2715)
                     /locus_tag="IVM62_001020"
     CDS             complement(334..2715)
                     /locus_tag="IVM62_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6844992.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2705..3352)
                     /gene="recU"
                     /locus_tag="IVM62_001021"
     CDS             complement(2705..3352)
                     /gene="recU"
                     /locus_tag="IVM62_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP6844993.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3416..3955
                     /locus_tag="IVM62_001022"
     CDS             3416..3955
                     /locus_tag="IVM62_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP6844994.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4045..4464
                     /gene="gpsB"
                     /locus_tag="IVM62_001023"
     CDS             4045..4464
                     /gene="gpsB"
                     /locus_tag="IVM62_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP6844995.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4486..4853
                     /gene="rnpB"
                     /locus_tag="IVM62_001024"
     ncRNA           4486..4853
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVM62_001024"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5062..6228
                     /locus_tag="IVM62_001025"
     CDS             5062..6228
                     /locus_tag="IVM62_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP6844996.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6274..7770
                     /locus_tag="IVM62_001026"
     CDS             6274..7770
                     /locus_tag="IVM62_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP6844997.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7851..8534
                     /locus_tag="IVM62_001027"
     CDS             7851..8534
                     /locus_tag="IVM62_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6844998.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9179..10369
                     /locus_tag="IVM62_001028"
     CDS             9179..10369
                     /locus_tag="IVM62_001028"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP6844999.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10471..11424
                     /locus_tag="IVM62_001029"
     CDS             10471..11424
                     /locus_tag="IVM62_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP6845000.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11493..12842
                     /locus_tag="IVM62_001030"
     CDS             11493..12842
                     /locus_tag="IVM62_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845001.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13025..13348)
                     /locus_tag="IVM62_001031"
     CDS             complement(13025..13348)
                     /locus_tag="IVM62_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845002.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13477..14640
                     /locus_tag="IVM62_001032"
     CDS             13477..14640
                     /locus_tag="IVM62_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845003.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14710..16059
                     /locus_tag="IVM62_001033"
     CDS             14710..16059
                     /locus_tag="IVM62_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6845004.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16104..16535)
                     /locus_tag="IVM62_001034"
     CDS             complement(16104..16535)
                     /locus_tag="IVM62_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845005.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16923..19565
                     /gene="alaS"
                     /locus_tag="IVM62_001035"
     CDS             16923..19565
                     /gene="alaS"
                     /locus_tag="IVM62_001035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP6845006.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERIVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19765..20361
                     /locus_tag="IVM62_001036"
     CDS             19765..20361
                     /locus_tag="IVM62_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6845007.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20483..21631
                     /locus_tag="IVM62_001037"
     CDS             20483..21631
                     /locus_tag="IVM62_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6845008.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21669..22082)
                     /locus_tag="IVM62_001038"
     CDS             complement(21669..22082)
                     /locus_tag="IVM62_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP6845009.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22161..22592
                     /locus_tag="IVM62_001039"
     CDS             22161..22592
                     /locus_tag="IVM62_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP6845010.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22691..22924)
                     /locus_tag="IVM62_001040"
     CDS             complement(22691..22924)
                     /locus_tag="IVM62_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP6845011.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23107..24777
                     /locus_tag="IVM62_001041"
     CDS             23107..24777
                     /locus_tag="IVM62_001041"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP6845012.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24924..25310
                     /locus_tag="IVM62_001042"
     CDS             24924..25310
                     /locus_tag="IVM62_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845013.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25297..25995
                     /locus_tag="IVM62_001043"
     CDS             25297..25995
                     /locus_tag="IVM62_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6845014.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26000..26464
                     /locus_tag="IVM62_001044"
     CDS             26000..26464
                     /locus_tag="IVM62_001044"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP6845015.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26461..27372
                     /locus_tag="IVM62_001045"
     CDS             26461..27372
                     /locus_tag="IVM62_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6845016.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27607..28146
                     /gene="pyrR"
                     /locus_tag="IVM62_001046"
     CDS             27607..28146
                     /gene="pyrR"
                     /locus_tag="IVM62_001046"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP6845017.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28160..29443
                     /locus_tag="IVM62_001047"
     CDS             28160..29443
                     /locus_tag="IVM62_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP6845018.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29456..30382
                     /locus_tag="IVM62_001048"
     CDS             29456..30382
                     /locus_tag="IVM62_001048"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP6845019.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30401..31681
                     /locus_tag="IVM62_001049"
     CDS             30401..31681
                     /locus_tag="IVM62_001049"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP6845020.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31686..32762
                     /gene="carA"
                     /locus_tag="IVM62_001050"
     CDS             31686..32762
                     /gene="carA"
                     /locus_tag="IVM62_001050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP6845021.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32767..35952
                     /gene="carB"
                     /locus_tag="IVM62_001051"
     CDS             32767..35952
                     /gene="carB"
                     /locus_tag="IVM62_001051"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP6845022.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(36281..37600)
                     /locus_tag="IVM62_001052"
     CDS             complement(36281..37600)
                     /locus_tag="IVM62_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP6845023.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT        12123 a         6662 c         8129 g        10821 t
ORIGIN      
        1 aaatgaagcc ttctttcaaa aaattattca aaaccatata ttgtattttt tatttgataa
       61 taacaatggg attattaaga agtggcacta aataaaagac tagtgtgtat ttagccgatt
      121 taattataaa caagtaaatg gataaagtat atgtaatgat aaaagaaaaa tggattttct
      181 aatttttaag ctaaatgtta gatcacacat gtatatgctg gatagtctta taaaaaatca
      241 tgtaatacag cttggtacat gatttttatt tgtagctgtt ttttacatag acacaaaaaa
      301 gaaaccacgc ttgtagacgt ggtttctttt ttactatctg tttccagaag ctgaggtgct
      361 gctggatggt gtatggtcat ttgcagcatc cgctccgcta gttgctggct cactggaaga
      421 agcaggagga gcagatgaag acggtgtcga gctttcttcg gatgaagaag aaggaaccgt
      481 tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt
      541 tgattcgctt tgactagtag agctagagct ttgagtcgtt gtctctgttg ttgagcttcc
      601 aggagtctgg acatagctgg aagatggaat agttgttgat ggtgtaattg cattcgatct
      661 agctgtatat tgatctttgt agtacagttc accgcccaca cgaattaatc cgcttggcat
      721 ctcccagtct gtatttgtca cgttagctga aacatactgc ataagctcgc gataaacatc
      781 agaggctacg tgtgacgatt ctgaagtgac aggtgtcatt tttttattgt aacctgtcca
      841 cacggaaatc gaataatttg gcgtatagcc cgcaaatagg atatctggat acaccccaga
      901 agaaatacca agttttgcat actcgtcatc tgtgtaattg gatgttccgg tcttaccggc
      961 ttgatataac ccagcgattt gcgcattcgt accagttcct tcagtgatcg tatcttttaa
     1021 aatgtctgtg atcatataag ctgtttctgg agacatggct gtttttccat ctggctcata
     1081 agtttcttct gttccgtctt ggaagacgat tttattaaca tattgaggtt tgtaataagt
     1141 ccctccattg gcgaaagccg cataggctgc tgccattttt aaggaagaag ctccgtattt
     1201 tgtcccatct tgttcttccg tattactaga gatcgcattc gattgatgga tcgtactgta
     1261 ttcgatccca agatttttta agaaagaagc cactttatct gaaccgactt cattgaataa
     1321 tttgactgct ggaacattac gggataagta aagtgcttgg cgtaaagtaa tggtgcccat
     1381 atattgatta tcccagtttc ctacaggagt cgaagtccct tcatagttat acggtgcgtc
     1441 agtaatagtt tttccagtag aatatttcaa atattcaaaa gctggtccgt aatctgtgac
     1501 aggtttcatc gtagaaccaa aatcacggga agtattcact gctaagttgt tgcctaaagt
     1561 tacgtcttct gcgatgtgac gtccgccaat ctgggctttt actttcccgg tattggtgtc
     1621 gataagggtg gaggcaactt gcatctcgtc gtctgggtaa gagacatact gatccgtgtt
     1681 gacgatatca tatagtttct tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc
     1741 gtctgtgtag acattcttat ctgttttttc ttgtacttca ttgatgactt ctttgacata
     1801 attatcgacg atcttagtat tgtcatcgga ttgagttagt tcttgcagac catccgttac
     1861 cggtacatta acagcttgat catattctgt ttgagagatt ttttcgtttt gaagcatcgt
     1921 atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga taaacataag gatcataggc
     1981 ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa
     2041 tttcttacca aagtacatct cagaagctgt ctccatcccg tacaagccgt ttgacatata
     2101 cactttattt acgtagtagg tcaagatctc ttgtttcgat tttttttgtt ccaatcttac
     2161 agccatccac gcttcttggg ccttgcgttt cagagtctga tcctcggcac tggtggagaa
     2221 gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt
     2281 aaagttagag agggcagaac cgatgatccg aatagggtca accccgatgt gtttataaaa
     2341 acggcgatct tctaccgaca cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga
     2401 aattttttca cgcttttctg cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag
     2461 tttggaagat acggtagcgt ctaatttttt atccgtcaat tcgggcgcat cttttgcgta
     2521 ataccagaaa agtcctacac ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa
     2581 aaggatcttg ataaataatc cttttttatg tgttccagag gactttccag aacctttgcc
     2641 agaattcttc ttcacactct ttttaggagt aggctgtttc tgtcgtctag agcttctagt
     2701 ttgttcattt gccattcttg ctttctcctt caatagataa tgttgataaa tagaagtcaa
     2761 ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt gatctctatt cctagttctt
     2821 cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa
     2881 tcaaatgaac actattcaaa taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa
     2941 agcagacccc attttgttcg aggcatgctt tcatatgttc gatttgatgg gcatggaagt
     3001 ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc
     3061 ctcgatagac gccattatag tctgtagttg aggcttgggt aaagtaggct tccttgatca
     3121 cagcagcact tctcttagga tagtctactt tcacgatttg gataggggta ggctttttat
     3181 ggataaccgc caacccgcgg gaaagataat atttattact ttcattgatt gcttcttcaa
     3241 agcgcatccc ccgattgccg aattcagtga tctgatttct tttttcttga attttttcga
     3301 cgggagaggc attcttataa taaggttggc catttggata gcgcaatacc atctttaatc
     3361 acactcctag gccacattat agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga
     3421 taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc gaactcggtg tttttaaaga
     3481 aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc
     3541 agaagaaggg ctggaatggg tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt
     3601 gcaaatagta gcggaattga aagaagaata tccggaacta tctcttggta ttttgtatcc
     3661 tttttctgat tttggtaata actggaatga agggaaccaa gaaaaaaaac aattagcaga
     3721 acaattagca gattatgtcg aagctgttag ccatcaaccc taccaatcac ctagtcagct
     3781 aaagaatcac actcgtttta tacttgagca tacagtgggc tgtctgctga tctatgatga
     3841 agaattccct ggaaaaacga agtatttttt tgaagatgca cagaattacc aagcagatca
     3901 tccctacgaa attcggctga tcagtatgga cgatcttcaa aattttagtg aataatcgat
     3961 attgtgtttg atttatcaac ttttttccta tacaatgaaa aaggtagaaa tgttattacg
     4021 taaatgaaaa ctagaggtgt aaatatggcg aatttggttt atagtcctaa agacatttta
     4081 caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg ttgaagttga tgaattctta
     4141 gacaatatca tcaaagatta tgaaacttac agcaaagaac tccttgcttt acaagaagaa
     4201 aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca
     4261 cgcgtgcaac aaacagaggc accaaagagc gcagcagtga ccaattttga tatcttaaaa
     4321 cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca
     4381 gtcaaaccag cacaacctaa tccaaataac tacacaaatg cagacacaag cttagatgat
     4441 aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat ttgcggtttt cgggtaatcg
     4501 cggtctgact tgatcaggct gaggaaagtc catgctcgca caggctgtga tgcctgtagt
     4561 gttcgtgctt agcgaaacca taagctaagg tactctttgg agtaacggca ggaaaacaag
     4621 ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg
     4681 aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc aacccaaaca atggtagggg
     4741 cgctttttgt ctggaagaag aacaggacaa aaaggcagta taactgcaga tagatggtta
     4801 ccgctttgtt ttttctccct ggaaaagcaa agtacagaac atggcttata gaaaaccgca
     4861 aacatcaggg aattaggtcg tgacaaaagt aatgcgttat aagaaacaag aaaaacattt
     4921 gaaagctatt tttcaatact tcagatgaat cgttcttatt acaaaacaaa cttttgtatc
     4981 accggttatt cggaatttga gtaaaagtgt ggggtaggac tgatgtccca cctccttttt
     5041 ttataaataa ggagattgtg tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg
     5101 gcaagcgggc tagaagcact ggttggtaaa gaattaagag atcttggaat cgaatgccaa
     5161 gttgagaacg gacgtgcacg ttttgaagga acaatggaaa cgatcgctac agctaattta
     5221 tggttacgta cagcagatcg agtaaaaatc gtggttgggg aattcgatgc gtatacgttt
     5281 gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag attttcttcc actagatgca
     5341 gcttttcctg tagctggaaa atcgatcaag tcgaaactat atagcacacc tgattgccaa
     5401 gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct attatcaccg tccagcttct
     5461 gtaccactga cggaaactgg cgcacatttt cagttggaag tagctttgct gaaagaccat
     5521 gtgatggtaa ctctggatac gacaggacca agtttattca aacgtggtta tcgcttagaa
     5581 aaaggtggag caccattgaa agaaaacatg gcagcagcac ttattatgtt gacaaattgg
     5641 cgaaaagacc gtccatttta tgatcctgta tgtggttcag gtacgctttg tattgaagca
     5701 gccttaatag gccataatat cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg
     5761 gattggttca gtcaagaaat catggaaaat gttcgatcag cagccgaaga aaaagcagat
     5821 tatgatattg aattagatat tactggttca gatatcaatg gacggatgat tgaaattgcc
     5881 aaagccaatg cagaagaaat cggattaggc tcttcgatta cctttaagca acaagcagtc
     5941 aaagacttca aaacggataa agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa
     6001 cgtctaggag aagaagaaac tgttcggaaa ttatataaag aaatgggcga tgtattccgt
     6061 ccattaaaaa catggagtaa atatatcttg actagcgact tagcgtttga ggaattttac
     6121 ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg cattgcgtac ggatctattc
     6181 cagtattggg gaactcgtcc gcctagaaaa aaagagaaag acgaataatc ttactaattg
     6241 attcattcaa ttggatgttt ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa
     6301 gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc tcgattggga tacacaaaca
     6361 ggtatgcctg aaaaagctag tgcataccga agtgaagtag acagctatct ttacagcatt
     6421 tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca ttcgctattt ttctgaacat
     6481 ccggaagaat tgtcggaagt tggaactgcc gtttttgcca aagtaaaaga agagtatgat
     6541 ttggaacatc gcgtaccgga agatcttatg agaaaatata cagctgccgt ttcttcagca
     6601 cacggacaat ggcaaaaagc tagagaaaca caacaatttt ctgattttca acacgcattg
     6661 aatcaaaata ttgaattaac aaaacagttg ataccttact ggagaaaaga tgaaaagacg
     6721 aattatgatg ttttgctgaa tcaatacgaa cccgggatga ctgtcgaaat ccttgataag
     6781 gtatttgatc agcttaaaga aggaattctt tcgatccgcc gtacgctcca agaaaaaggg
     6841 actgaacctg aaacagactt tttaaatcgg agaatgacga aaaaacaaca gaagagattt
     6901 gtagtgaaag taatcgaaca acttggttat gattttagtc gtggtcgttt agatgacact
     6961 gtccacccat ttatgactgg aattaatccg aatgatgcac gaatcacgac acgatggaac
     7021 gaaacagatt tcaaaatggc tgtttttgga gtgatccacg aggctggtca tggtatgtat
     7081 gaacaagaca tcgatgataa atatgcgtat acaccggttt atgaaggtac atcaatgggt
     7141 atccatgaat cacaatcgtt gtttaacgag atcattattg gaagtagtcg cagcttctgg
     7201 aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa cattcgatga tattcctttt
     7261 gacaaatttt atcgttcatt gaaccataca aaatcaagtt tggttcgtat cgaggcagac
     7321 agtttgactt accctctcca tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat
     7381 gaaggggtag atgttgctga actacctgaa atatggaatc aaaaatatga agagtatctg
     7441 ggaatacgac caacaaatga tgtggaaggc atcctacaag acgttcattg gtcaggtgca
     7501 agctttggtt attttccctc ttatgcatta gggtacatgt atgcggcaca attacttcat
     7561 tctttgcagc aagaaataga tgtggaaaat gttctagcgt cagacgatta ttctccaatc
     7621 aaaaactgga tgtcagagca tatccatcaa tatggtgcat ctagaaaacc aaatcaatta
     7681 atcatggatg caacacatga accgcttaat ccgcaatacc tgatcgacta tatgaaagac
     7741 ctgtatttta atgtatatca ggtaaaataa aatacaattt acaaagatag ctgaaattca
     7801 tctaatctat agatgttttc agctatttac ttaagaaaga agtggaacga atggataaat
     7861 tacaagtgat cgctgattat agtatggacc aattagcgca tgatcaaaca ggacatggga
     7921 gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg
     7981 cagaccgctt tgtgacgctt gcagcagctt acctacatga tacgattgat gataaagtcg
     8041 ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag
     8101 tagaggaaca gatctcgatg attttttcca ttatcgaaaa tatgtccttt tctaaaaatc
     8161 tctctgaagc ggccgaacta tctctggaag gtaagatcgt tcaagacgct gatcgtatcg
     8221 aagcattagg agcaatcggc attttgcgta ctgcttattt cggcggtgga catgggcacc
     8281 cgatttttga tcctgaactg tatccgcaaa cattcaagga caaagaaaat taccgaaaag
     8341 gcacgacagt gatcaatcat ttttatgaaa aactgttctt gctgtctgat aaaatgaata
     8401 ctgattacgg atatgaagaa gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag
     8461 aattttttct ggaatggtct attgacgatg aaaattttac tccagacaat tggatcaaaa
     8521 ataacaaaaa atgatggagg ttgtgacaga agtggtcagc tccaagatat ttcggatcat
     8581 tttccgtaaa agttacttct ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag
     8641 ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg tgtttgatga atattgtaac
     8701 ttgtgattcg gaattggcag gagaacaccg tttattcgga agaataggac tgtgacaaga
     8761 cttttgtcac agtccttttt tcgaattcaa caagtaaaac ttttaagaaa tattcagaga
     8821 gtaaaattaa aaattgctga taaacgaaga tggaaatttt atgtattttt attggtctat
     8881 gcaataaata aattaaatat tcagaataca attgatttta acgaaagggc ttctatcaat
     8941 ggttgtgttg tatttatctg cccaggtaga gtaaaataga tcattatcta aaaggtaatt
     9001 gttcatacta tataagtcat ttgaaagtaa aaagatactg taaataaaaa atgtggttaa
     9061 ataattctca taatcagtgt aagcgttatc aaaaatatag catacttgct ttgtagatga
     9121 atgacacgat atatgtttgg tatgttctgc aagacagatg aatatggagg tggagacaat
     9181 gtcttccaaa gaactaacaa tgtatttaga gaaagaattg gatcaactga aagaaaaggg
     9241 gttatacaat acgatcgaca tactagaaag tgaaaatggt gcttgtatca tcgtagacgg
     9301 aaaaaagatg atcaatctag catccaacaa ttatcttgga tttgccaatc gcgaagaatt
     9361 gaaaaaagca tgtattgaag caaccgaaac ttatggtgta ggagcgggag cagtccgaac
     9421 gatcaatggt tcgctgaaaa tccatcagca attagaagaa aaaatcgcag aattcaaagg
     9481 gacagaagca gcaatcgcct ttcaatctgg attcaactgc aatatgggcg ctatctctgc
     9541 agtaatgaca aaagaagatg ctatcttatc ggatgaatta aaccatgcat ctattattga
     9601 cggttgccgc ttatccggag caaaaatcat ccgaattaaa catcaagata tgaaagacct
     9661 tgagaaaaaa gcaaaggaag cgatagaaag taagaaatat aaaaaaatca tgtatatcac
     9721 agatggtgtt ttctccatgg atggagacat tgcacgattg ccggaaatta ttccgattgt
     9781 tgaaaaatat ggactgatca cctacgtcga tgatgcgcac ggctcaggcg taactggaaa
     9841 aggtgcagga accgtcaagc attttggatt aagcgacaaa atcgatatgc aaatgggcac
     9901 gttatcgaaa gctataggtg ttgttggggg ttatgtagct ggatcgaaaa cactaatcga
     9961 ctggttgaaa gctagaagtc ggccattcct attttctact tcgctaactc caggtgcagc
    10021 tgcagctgca ttggcttcga ttactttgat gcaagaacat ccggaacttg tcgaaaaagt
    10081 ttgggaaaat gctaattatt ttaaagaaga attgaaaaaa gtaggctata acatcggtat
    10141 gtctgaaaca cctattactc cagtcatttt gggagacgaa aaagtgactc agactttctc
    10201 taagaagctg atcgaacatg ggatttatgc taaaccaatc gtttatccaa ctgtgcctct
    10261 tgggacaggc aggatcagaa atatgccgac tgcggaacat acgagggaaa tgcttgatga
    10321 agcagtagca gtttatcaaa aaataggaaa agaaatggaa attatttaac taaatttagt
    10381 tttttcgtct atttaaaatt agagaacatg ttcacgcata tattgttaat catctgaaga
    10441 tagtagtaaa aaagatcagg gaggatcatc atgaaaaaag tattggtgac aggatgttta
    10501 ggacaaattg gttcagagtt gatcacacgg ctacgtcaag aactaggcag tgaaaatgtc
    10561 attgcgacag atatacgcat gcctgaagtc attgagaaaa acgatatctt tgagatccta
    10621 gatgtaatgg attacgaaaa aatgcgcagc atagcagaac aatatcaagt ggacacattg
    10681 attcatttgg ctgctctttt gtcagctgtt gcagaagcaa aaccacaatt agcctggaat
    10741 ttgaacgtga ctgggctggt caatgcattg gaagtcgcta gagaattcca gctgaaattt
    10801 tttacgccaa gttcaatcgg cgcgtttggt ccaaatactc caaaagatca taccccacaa
    10861 gatactcttc agcgtcctac aaccatgtat ggggtaacga aggtagctgg agaactacta
    10921 tgtgattatt attatgtgaa atatggcgta gatactcgtg gtgtccgttt ccctggattg
    10981 atttcttatg caaccttgcc aggaggcggt acgacagact acgcagtcga tatttattat
    11041 tcagcaatca aagaaggaaa gtacactagt ttcattgatc aaggtacatc aatggatatg
    11101 atgtatatgc cagatgcgat cgatgccatt ataaaactca tgaatgcaga tcctgatcga
    11161 ttggttcatc gaaatgcctt caatatatcg gcgatgtctt ttgacccgga agagatcaaa
    11221 gcatctattc aaaaaatcat accagatttt gaaatggatt atgatgtgga tcctgtacgt
    11281 cagtcaatag cagaatcttg gccaaattca ctagatgttt cttgtgcgca agaggaatgg
    11341 ggcttttcgc cgaaatacga cttagatgtg atgacaaagg atatgttgga aaaactgaaa
    11401 gaaaaattgg tagagacggt ataaaacaag aaccacatcc aaaaagtttt ttatactttt
    11461 gggattggct atggtatgaa aggggacaag ggatggaaga atatcaagcc acgccagtaa
    11521 aagaagtaaa aatagaaaat gaattaaaac gtacgatggg attttttact gctttatcga
    11581 ctgttatggg cacagtgatc ggtgcagggg tattttttaa agccgcaagc gtagcagaag
    11641 ttacaggatc cgccagtttg catatgtttt cttggtttct cggtggtatg atttctgtct
    11701 gtgcaggact aacaggagct gaactggcag ctgctattcc agaaacagga gggatgatta
    11761 aatatatcga acggatttac ggaaatacag cagctttttt attgggctgg gcgcaagtcg
    11821 tgatttattt tccagcaaat gtagcagcct tgtcgatcat tttcggtaca cagttcgtca
    11881 atctatttgg tctttcccag tcaatgattg ttcccgtagc tgttacagct gctgtatcga
    11941 tcctgttgat caatttttta ggttctaaag ctggaggggc ttttcagtcg attacgcttg
    12001 tctgcaagct tattcctttg tttgtgatcg tcattttcgg tttgttccga caagaaggag
    12061 tagacttcca actgtttccg atccaagcag gagaaaatct ttcatttttc tcagccttgg
    12121 gagcgggctt gctagcaacg atgttcgcat acgacggttg gatacacgta ggaaatattt
    12181 cgggagaatt gaaaaagccg gcgaaagatt tgccaaaagc aatttcttta ggaatcatcg
    12241 gaatcatgat cgtttattta ttagtaaatg cggtgttctt aagaaccgca tctatcgacg
    12301 gagtcgcagg aaacagcaat gcggcaagtg acgttgcaaa aatgattttt ggcggcttcg
    12361 gtggaagact ggtcacagtt ggtatcttga tttctgttta tggaaccatc aatggctata
    12421 cccttactgg aatgcgtctc ccttatgtta tggcaaaaga aaacaacctg ccttttagca
    12481 agctctttgc taaacttcat gataaaacta aagttcctgt agccgcaggt atcttagagc
    12541 tagtcatcgc tattggcatg atgatgatcg gtggttttga tacactaacg gatatgctga
    12601 tctttgtcat ttggattttt tatacgatgg tctttgttgg tgtcattctt cttcgtaaaa
    12661 aagaacctga tttgtttaga ccatacaaag ttccaatgta tccgtttatt ccactagtag
    12721 caatcattgg agggactttt atcgtatcaa gcacgcttat cactcagacc tttttagctt
    12781 cgatggggat agtattgaca cttgctggta tcccgattta tctctattta aaacgtagat
    12841 agctaaaaca ttcgaatttt attagaactg cacttctctg ccaaacaaaa tattttattt
    12901 aaatgaggag tacgtggaag aaatgtcaaa agtactcgtt aattttgatc acgtaaatgt
    12961 cgttttgtct acgatgaaat agcaaagtaa aagacagaaa tcgggctcaa ccgatttctg
    13021 tcttttactt tgatttgcgt aactgaacta ttcggcgtac gccgcgataa atatttaata
    13081 aaaccaagca accagaaatg aaaatgggaa gccaaatcag atagacaggg tagccaagca
    13141 ctttagacaa agcatgtgga gtcattttat ccatttcaag aagtgcttgc tgccaagtct
    13201 gaagattgaa gactagcatg aaacccgaaa taatcaggag aataaatcct aaataatgaa
    13261 ttgtagttct ttttcttaca taagcgatcg tagcataaag gaacagtaaa aaaataggta
    13321 cgccaaaaac aaagtacata ttcagcatct cgacaccccc tttgtgttta tcttacttta
    13381 ctttgaaaga aatgagaaat aatagcctcg ttacttgctt gaaacagcaa aggactgcta
    13441 agataagaga gatacaaaga atcaaggagg agtcatatgg caagttatat cgaaccgatt
    13501 cgttttgcgc tgattgtttt tccattttta gccttagcga tctcatctgt tttttttatt
    13561 tatgaatatc gaaagtatgg aacatttcta gtaacaaggg cagttatttt gtattctttc
    13621 gttttttatt tattgtgtgc gtattttttg gtgatcttac cacttccggc aaaggagacg
    13681 gttgcccaaa tgacagggcc aaagatggag ctgcatttat ttgcttcatg gcaacatttc
    13741 agagacaaca cggtattggt cttgtctgaa cctagtacct acctgccggc aatgaagcag
    13801 agtgtatttt tggaaccagc ttttaatgtt ttattactca taccatttgg catttattta
    13861 agatattatt tccggctttc ttttatgaaa acgatgattg ctagtttctg cctttctcta
    13921 ttttttgaat tgacgcagct aagtggactt tattttatct acccaagacc ataccgctta
    13981 tttgatgtga atgatttagt gacaaataca ttgggtggaa tcattggttt tgtcgttacg
    14041 ccgttattta catttatgct tcctacaaga agacgaatgg atgaactttc ttatgaaaaa
    14101 ggaaagactg tctcattgac tcgacgcttg gtcgcttatt tgatcgactg gacattttta
    14161 tctcttatta atttggtagc ggcgattttc tttaaaattg cgttcgattc aggggcagtt
    14221 acttctcata tctggtggct tttattagaa gtaggggtat actttatgat tgtaccgttt
    14281 ataaccaatg gatggacact tggcaaaaaa attgtgcgta tccgtttggt ggaagaagac
    14341 cgcgaacgaa tttctttaaa agcattgatg atccgctacg ggtatctgta ctttcttttt
    14401 tatggtttaa gctggttggg gcagttgctt cagcaaatca tgaattcgac aacgaatacg
    14461 gtgttgcaga tgagtgcagt attcatttct ctcggtgtct tgttactaca aggtattttt
    14521 attttaaata tcttgctatc atttttgaga aaaaaacgga ttctgtttta tgaaaaagct
    14581 agtcacacat atactgttag cacgattggc caaacaaagg atgatgattc tcgaaattag
    14641 ctgagcaaaa acgctttgtc tgtgttataa tgccaaagtc gtgataattt aaagagaaga
    14701 ggtgcacgaa tgaataattt tgatatattc cgcttcaaac cgtttatcaa tgaagcgtta
    14761 accgcaaagg gcttcaaaca gcccacagag gtccaggaac gtttgattcc tttaattgca
    14821 aaaggaaaaa atatcattgg gcaatctcaa actggtacag gaaaaacaca tacgtttttg
    14881 ttgcctttgt ttgaaaaaat caatgcagaa aaagaggaag tacaagttgt tattacagct
    14941 ccaagtaggg aactagctgc acagatctat gaagcagcta cacaaatcgc taaattttct
    15001 acaccggaga tccgtgtctc gaattttgtc ggcggtacag acaaacagcg tcaattaaat
    15061 cgtctgcatc accaacagcc acaggttgta gtaggcacgc ctggtagaat attagacatg
    15121 atgaatgaac aagcgctgaa gattcacacc gcatttgcat ttgttgtcga tgaagcggat
    15181 atgacattgg atatgggttt tttaaatgaa gtagaccaaa ttgcaagtcg attaccagaa
    15241 aagctgcagt ttttagtttt ttctgccact attccagaaa aacttcgtcc attcttacga
    15301 aaatacatgg aaaatccaat cattgaagaa atcaaaccaa aagcagttat ttctgaagac
    15361 attgaaaatt ggctgatttc aacaaaaggt aaagatgaga atcaaatgat ttatcaactg
    15421 ctgacaattg gtcatcctta tttagcaatc gtatttgcca atacaaaaca gcatgtggat
    15481 gaaattgccg attacttgaa aaaccaaggc ttgaaagtag caaagattca tggagacatc
    15541 accccaagag agagaaaacg agtgatgcga caagtacaga atctggagta tcaatatgtt
    15601 gtagcgactg atttagctgc tagagggatc gatatcgaag gtgtctctca tgttattaat
    15661 gcagaaattc cagaagatct agacttcttt atccatcgtg tcggacgtac tggacgtaat
    15721 ggcttaaaag gaacggccat tacgttatat tcgccaaatg atgagcaagc cattgatgcg
    15781 atagaaaaat tgggcgtctc atttgaacca aaagaaatca aaaacgggga aatcgtcaaa
    15841 acatacgatc gtaatagacg tgccaaacga gaaaaatcaa aagatgtatt agatcccacg
    15901 ctgattggtc ttgtgaagaa gaaaaagaaa aagatcaagc ctggttataa gaaaaaaatc
    15961 gattgggcaa ttgcagaaaa aaataaaaaa gaacgcaaaa tcgaacgcag acagcaaagc
    16021 cgtactgctc gtaaaaataa gaaaaattca cataaataaa aaacaagagg ttgtgacaaa
    16081 aggcgtgtca caatctcttg tttttaagct agactttttt tcattccgat acaaagtact
    16141 tcatcttcaa taaaaggttc tgtgcaagta tgataccctt gtttttcata aaaatctaca
    16201 gccgtcattt cagctactac ataactagtc tgacaacctt ctgaaaccgc ccgtttttca
    16261 gcagcttcaa gcaactgacc gcctaaccct tttcctcggt attcttttgc tacgcaaaat
    16321 cgttccggct tgaagcaagt aggtgttttc ttttcataac gcaaagtagc tactggtctt
    16381 ttttgatcaa gaaatagata ataggtttgt tgcgggatat cttttccgtc gaattctagt
    16441 tctgcaggaa tcccttgttc taatacaaaa acttgatatc tcaaaaagca gctagctgcg
    16501 cgtatccatt catccgctcc aactattttt ttcatcatat tcctccttgt catgaatagc
    16561 agagatgtat tgattccttt attcttatcg taccaaaaat cgtggagtat gaccaaaagg
    16621 aaatgaaaat aaaaattgag atattgacag tgatcaatag aatgcgtata ctaattaagt
    16681 acgaaaagga tatgatttgt tttttaagac tcacagaaaa ttctttcttg gctgaaaaaa
    16741 gaatagtcac taatcaaatt gctccctttt tctattttgg tagaaatacc gacgcagctc
    16801 agcgttatcg agtttaagag gtaatgatta cacatcattg caatcaaggt ggtaccgcga
    16861 gttttcgtcc ttggctgtga tggtgttttt ttgtatttaa tttatagaaa gcaggtaatg
    16921 acatgaaaca attaagtagt gcacaagttc gacaaatgtt tcttgatttc ttccaatcta
    16981 aaggacatac gattgaacct agtgcgtctt tgatccccgt gaacgatcca accttattgt
    17041 ggatcaactc tggtgttgca acattgaaaa aatattttga tggttcagtt gtgccagaaa
    17101 atccaagaat cacaaatgcc caaaaatcta ttcgaacaaa tgatatcgaa aatgtcggta
    17161 aaacagcacg gcaccatacg atgtttgaga tgctgggtaa tttttcgatt ggtgactatt
    17221 tcaaaaatga agccatccac tgggcatggg aatttttgac tgatccaaaa tggatggcat
    17281 tcgatccaga aaaactttat gtaacagttt atccaaaaga tacagaagca aaacgcatct
    17341 ggcatgaaga agttggcctg ccactagatc acattattga tgtagaagac aacttctggg
    17401 atatcggtgc aggcccatgt ggtccggata cggaaatttt ctatgaccgt ggagaagctt
    17461 ataacgattt agcagaagac gatcctgaaa actatccagg aggagaaaat gaacgttatt
    17521 tagaaatctg gaacttagta ttttcagaat tcaaccataa gcctgataac acatttgaac
    17581 cacttcctca taaaaacatt gatacgggga tgggattgga acgcattgtc tcaatcgttc
    17641 aagatgcgtc aacgaacttt gagacagact tgtttatgcc tattatccac gccgtagaaa
    17701 agctaagcaa acaagtaaaa tatggtgaaa acccagtaac agatatttct tacaaagtaa
    17761 tcgcagatca tattcgcgcc ctttcatttg ctattggcga cggtgcattg ccatcgaatg
    17821 aagggagagg gtatgtctta cgccgtttgc ttcgtcgtgc tgtcatgcat gggaaaaaat
    17881 taggtattga acaagcattc ttatatcgtt tagtccctgt tgttggggaa atcatggtaa
    17941 gttattatcc tgaagtactt caacaaaaag aatttattga aaaagttatc cgtacagaag
    18001 aagaacgttt caatgaaaca atcaatgaag gtttggaaat cttaaatcaa gtgatccaag
    18061 aagtaaaaaa cgaaaacggg acgactctag caggaaaaga catcttcaaa ctatacgaca
    18121 catatggctt cccggtagaa ttaacggaag aggtggcaga agatgaagga ctgaaagttg
    18181 atcatgctgg ttttgaaaaa gaaatggatg cacaacgcga acgtgctcgc tctgcccgca
    18241 gtactgaaaa atcaatgggg gtacaatctg ctttattgac agatatcaaa gtagaaagta
    18301 aattcattgg atacacagaa caagaatcag atagtcaatt attggtcatc gttaaaggtg
    18361 agtctctttt gaatgaagtc gcagaaggtg aagctcaaat gatctttgat caaacacctt
    18421 tctatgcaga aatgggtggg caagtagcgg atcaaggatg gatcatgaat gaagtaggcg
    18481 aaatcgtagc agttgttaca gatgttcaaa aagcgcctaa tggtcaattt atgcacaccg
    18541 tagaagtaaa agcaccaatg agtgaaggac aaacctatca tctattgatc gatcaatcga
    18601 tgcgcaatcg gatcatcaaa aaccatacag caacccatct actgcacaag gcattgaaag
    18661 aagtacttgg cgagcatgca aatcaagctg gttctttagt cgctcctgga catttacgat
    18721 tcgactttac ccactttggt caaatcactt cagatgaatt agctgaaatg gaaagaatcg
    18781 tcaacgaaaa aatctgggaa gctatcccag ttgaaacaat tgaaacagat atcgatacag
    18841 ccaaaaacat gggtgcaatg gctttgtttg gtgaaaaata cggacatgac gttcgagtag
    18901 taaatattgg cggctggtca atcgaactgt gcggaggcac acatgtagca aatacagaaa
    18961 acatcggaat cttcaaaatc gtttctgaat caggaattgg tgcaggcgtg cgacgaatcg
    19021 aggctgtaac aagtaaagaa gcgtacgaac tacttgaaga agaagaaaaa caactaaaac
    19081 aagtagcaac aattgttaaa tcacctcaat tgaaagaagt tgtttcaaaa gcggaacaat
    19141 tgcaacagca agtaagagag ctacaaaagg aaaatgaaca attagctagc aagttagcga
    19201 atcaacaagc tggtgatgta ttcaaagatc cagaaacgat caatggaaaa acaatcattg
    19261 cagcacaagt aaaagtgaaa gatatgaatc agctgcgtca attagctgac caatggaaac
    19321 aaaaaaatat ttcagatatt cttgttttag caaacgaaca agaaggaaaa gtcagtctgc
    19381 tagcggcaat gacgaaagaa tcaaatgaag ctggactaaa agctggcgac ttgattaaag
    19441 caatcgcacc gaaagtaggc ggtggcggtg gtggacgtcc agacatggca caagctggcg
    19501 ggaaaaatcc tgctggtatt ccagatgcac ttaaagctgc gaaagaatgg gttgcgcaac
    19561 aatagggcgt ttacgtttga cagaaaaaca aaagttcaaa cattgaacga aacatgatta
    19621 tgggggcgaa gacaaacaag tcttgccctc atactttttt caattggatg gcgcatctaa
    19681 tcaaatggtc ggattcggat ggtctttagt tgacgaaagt gacagaaatt tttacgatta
    19741 gtataaataa aaaggggtga aaaaatgaat agtagaaata agacctttcg actagtctta
    19801 cgtgcgatct tgttagcaat catcatcgta caagcgatgg ttccttggct ggggtttatt
    19861 ccattaggat tcattagttt aacaattatc catatcacag taattattgc tgcagtagta
    19921 ttaggcccaa aagatggcat ggtgattggc ttgttttggg gaatcgctac aattgttcgt
    19981 gcctatgcca tgccaacaac gccatttgat acattagtgt tcacgaatcc gattatctcg
    20041 gtagttccca gagtacttgt tggcttagta gcaggcttgg ttttccactg gatttatcaa
    20101 aaaaataaat caataacgat cagctcagcc tttgcagggg tactgggttc attggtcaat
    20161 acagtcttag tgttaggatt catgggatta ttttatacag gtgcaacggc agatgcctat
    20221 ggtgtagact ctgctctttt atttaaaacc ttagcagggg ttgcagcaat caatgggata
    20281 ccagaagcaa taggtgcggg aatcataaca ccattactag caaaagcgtt atttgcagca
    20341 acaccgttga aaccagaata atgaccatct tttaatagta ataggacgca cgatagatag
    20401 aaaaaggaag agaggagagg aaaacaatcg tcctctccat ctttttacct gagcaacaag
    20461 caaactagaa ggggtaaaaa acttggctaa gaaacaagaa gaaaaaaaga aacaaatacc
    20521 gcaacagcgg acaaaaaaag gagtactagc gggtatctta ggattggtta cgaatctgat
    20581 cttgtttgtc tctaagctaa tgatcgggct tttttcagga agtgtctcca ttatggctga
    20641 tgcagtgaac agtctatcag atactgcttc atccatattg actttgatcg gatttaaaat
    20701 tgctgcaaag ccagcagatc aggaacatcc ttatggacac gaacgcttcg aatatatcag
    20761 tgggttattt gtttcgatca tcattactta tgtaggattt caatttttag agtcttctgc
    20821 tgaaaaaatc atccatccag aaaatgtctc tttaacaccc atcgtctttt ttgtcctaat
    20881 catttccatc tttttgaaat ttgcacaagg caaaatgtat caggttattg cgcaatccat
    20941 tgattctgaa acattgagag ctacagcaac agatagttat aatgatgtct ttactacact
    21001 ggctgtattg ctttcagcag caatcgaatg ggtaactgga tggcgtattg atggatatat
    21061 aggttttgct ttagcattat atatccttta tagtggattg acgatgcttc gaaattttgt
    21121 atacgaacta ttaggaagtc gcccaacgcc agcagagatt gaagaaatgg aagagcattt
    21181 aaatgcttgc gatgatatac ttggctatca tgatttattg gtgcataact atggacccaa
    21241 taaacgcttt gcatcagtcc acatcgaagt agatgaatca atgaatttga atgtggctca
    21301 taagattatt gatctgatag aaaaagactt taaaaagtct ttggatgtgg aacttgtctg
    21361 tcacttggat ccaatgcctg ttgataacga aaaatactat aatatcttgc ggcagttaaa
    21421 gcaaattctt cttgaaacag gtacagggct taatattcat gattttagag taattcatca
    21481 aggaaaaacg ctgcaatttg atgtagtggt ccctgagaaa ttccagtttg ttgatacaga
    21541 actagaaaga atcttacgta gtcaaataga agaaaaaatc ggagattacc aattggaaat
    21601 tacgtttgat cacaattatc ttttatatta aatgagaaac tataaaaaga ctccttaaag
    21661 tttttaattt aaagagtctt tttttctttt aattgtttat ggagagcctg acgtgcaaga
    21721 ttatctgctc ctttattttt gttttcgggc aaccattgca ggatcaataa agaaaattcg
    21781 gatagaagtt cttggattct ttgaaggtag ggaaggaaga gctcgttctt cgttgcgttt
    21841 ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac agaaaatcgt ttcattctgt
    21901 aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga cttcgaactc tgcttgatgg
    21961 tttgtcccta tagaaagcgg gatagatagc tgttcttgtt taccttgata gacaatcaag
    22021 attccacctc cactaggacc agggttgcct tttgtagagg catcaatgta tacttttaac
    22081 ataaaaagag attctccttt ttgttcaaac taatgttatg gtaagattat atcattactg
    22141 aatcggtgag aggttagaga atgaaaaaaa atattcgatg gcagcctgaa ctggcacata
    22201 cgatcatcta ttggtcattg accttgatca ttctgttttt tagtctgatt ctttctttag
    22261 aaaacacagg tccttattgg aaaagcaatt ttgttttagg tatttttctt gtttttctat
    22321 ttctaggttt gcggcgttct tttattttaa aatcaaactc tgtgattata cgttatgctg
    22381 ctttttggaa agaccgagaa atatctgtcg gacagattcg atctttgacg ccaactaata
    22441 ctggggtgca aatgatgttg caagatgaga aagccccatc tgttttcatg atgcgaaaaa
    22501 aagatcaagt agtccttttt gatcattttg aaaaaaacgg acccaaatca gaatcaattc
    22561 cttatcctta tacgaaaaaa agaaaaagct aattttaaaa atcagaacct aaaaaaacga
    22621 atttttacag gaaaagaaaa gagtgagacg tacggttcat cccgtttagc gtctcactct
    22681 atttgtttat ctattcatgc atactagtat cggtagtctg aacgttgcct ttcttgacat
    22741 gggcagcttg tgttcctcga ttcccttcga tgatctcaaa ttcgaccact tgattttctt
    22801 ccaaagtttt gaatccctct tcttcaatcg cagtaaaatg tacaaaaatt tcttctgtgt
    22861 catcataact gataaagccg tatccttttt tgttgtcaaa ccattttaca attcctgtgg
    22921 tcaaagcact cacgaccttt ctaaagtctg atcatagcct tcattataaa gagtatttat
    22981 aagtacgtca aataaattaa aaacctgtaa ttttatgttt tttttctgaa taaaaaaagg
    23041 tataattggc atatcttaac aaagaagata ttttgttgaa taaagttcgt gaaaaggggt
    23101 aatcaaatga agaatgatct gcaaatttca caagaaacag agctagaacc gattgttgaa
    23161 gtggctagta aattagatct taaggaagac gatttggaac tgtatgggaa atataaggcg
    23221 aaaatcaatt tttctggaat caatcgatca aaggataacg ctgaggggca tttgatctta
    23281 gttacttcca tcaatccaac accagctggc gaaggaaagt caacgatcac tgtagggtta
    23341 ggagacgcgc tcaataaaat caataaaaaa tctgttattg cattgcgaga accctcactt
    23401 ggtcctgtga tgggaatcaa aggtggtgct gcaggcggag gatatgcaca ggtattgcct
    23461 atggaagata tcaacttgca ttttactggt gacatgcatg ctatcacaac agctaataat
    23521 gcattatctg ctcttttaga taatcatatt catcaaggaa atgaattgaa catcgatgcc
    23581 cgccgagtga tttggaaacg ggtagtcgat ctcaatgatc gtgaactgcg taaagtaatt
    23641 gttggcttgg gtgggccaat ccaaggtgtt ccaagagaag atgggtttga tatcacagtt
    23701 gccagcgaaa tcatggctat cttgtgtctt gcggcagacc ttgaagattt aaaggctcgt
    23761 cttgcaagga tcgttgtcgg ctatacattt gaccgtcagc ctgtgactgc tggagacttg
    23821 aaagcagaag gcgctctggc attactgctg aaagatgcaa tcaagccgaa tcttgtacaa
    23881 acaatttacg gaacaccagc ttttgtccac gggggaccgt ttgccaatat cgctcatggc
    23941 tgcaatagta ttttagctac caagacagca ttgagattag cagattacgt tgtcacagag
    24001 gcaggatttg gtgcagatct tggcggagag aaatttttag atatcaaagt acctaacttg
    24061 aaaaaagcgc cggatgctgt ggtgattgtc gccactgtcc gtgcattgaa aatgcatggc
    24121 ggaatgaaaa aagatgaact taaaaatgag aatcttgatg ctttgaaagt aggttttgca
    24181 aatttaaagc gccatatacg taatatggaa caatatcagc tacctgtgat cgtcgcaatc
    24241 aatgaattcg taacagatac agacaatgaa ctcacactgt tggagcactt gtgtgaaaat
    24301 caggggattc ttgccaagag agcgagtgta tgggcagatg gagcagaagg cggcgttgat
    24361 ttagcaaaag ctgtagtacg attgattgat agaaaagaag ctaattacaa gccattatac
    24421 cgcctagaag aaacgataca agaaaagaca gaaacaatcg tcaagaaaat ttacggagga
    24481 aatggtgttg ttttcagcaa aaaagcgcaa aaacaaatag aagagtttac gaagaatgga
    24541 tgggacaaac tgccgatttg tatggcaaaa acacagtatt cattttctga tgatccttca
    24601 ctgctaggtg caccggaaga tttcaccatc acgatccgcg aaatcatccc aaaattaggg
    24661 gcaggatttt tagtagcctt gactggagat gttatgacga tgcctggttt gcctaaaaaa
    24721 cctgctgcat tgaatatgga cgtgacaaat gacggagaag tgttgggatt gttttaagct
    24781 tgattataat ctaccatcga aggagctgta ttttgactac agcttctttt tttagacgat
    24841 agggaattaa ttttgtttac actaggatca ttctttttct ttattgtatt ttgagctata
    24901 ctaaagcata ataggagggt atgatgaaaa cgacatttat aagaaaaacc ggtttttacg
    24961 gtatgggtag ctcgattcag ctaagaatca acaatgaaaa acgcagtgtt ctcagtcatg
    25021 aacaaaagat aacgcttgat ctggaagtgc cctttacgat gcaggtcacc ttttattggc
    25081 taaaaagccc cgtttataca gtgaaagaag caaagcctgc ttatgtgatc acaatgaatt
    25141 acttgattct tcaaatctat ccgtttttat ttctagcaac tgggttgggg gccgttttcc
    25201 tacaaaattt atttttcagt ttttttgcag ttattgtgtt gattgcgttc tttttctata
    25261 taaaaaaccg ggcatatttg atcaaggagg aaaattatgg caaactctga tcttttttta
    25321 atcagtttca atcgaatcga aaaatggatg agagacgaga tgggaaatgc acgaaatatg
    25381 ggatttaccg aacttgtcag acgcctagct caaagaaaac aattaatgat cagaaaatac
    25441 gaagatgatc ttttacagct tgctcagtta aggaatgcga tcgtccatga tcggatcgca
    25501 gtcgattttg tcattgcaga gccaaatgat tggacagtca agcgaatcca gctgatcgaa
    25561 caagaactga ttcagcctga aaccgtgctg cctcgatttg caaaacgtgt aacaggtttt
    25621 gaacaagatc ttccgttact tgaactgtta aagatagtag cagaaaaaag gtattcacaa
    25681 tttccgttat acaataaagg aaaatttgta gcattgatta ctttacgtaa tatcgggttt
    25741 tggctagcaa aagaaagcca aaaaggtccg gttgatttaa caaataaacg agcgttggat
    25801 ttgatcatcc aaaacggaaa gtacacaaat tatcattttg ttccagctaa aacacatatt
    25861 tacgaggttg aagcgatgtt tcgtgagcaa gggactttgg aagccgtact tatcactaaa
    25921 gatgggaatc ccgatggtaa tctgctcggg atcgtacgac ctcgtgatat ttataaacaa
    25981 atagaaaagg attgattttt tgttaattat ttattggata ttcagcgctt tgctagtagg
    26041 agtggatcag ttggtcaaat ggtggatcac tgataatcta gcgcttggag agacgaaaaa
    26101 tcttatacca ggtattttgt cactgaatca tattcgaaac actggagccg catggagtat
    26161 gctagaaggg aagatgtggt tctttactat agtgacactc attgcagtcg ttgtgattct
    26221 cacattgatg attaaaaatc gtgaaaaagg taatcgctgg ttcatgattg gattgagttt
    26281 gattctagct ggtgcgattg gcaattttat cgaccgtatt cgtcttggct atgtagtaga
    26341 tatgttccag acagatttta tgagctttcc gatttttaac gtagcagatg tgacacttgt
    26401 atgtggagtg atttgtatgt taatttatat tattttagat gaaaaggatc aaagaaaaaa
    26461 atgacagaac ttaaaacgac gattcaccat gaatctggta ggatcgataa agtattgact
    26521 aatttattca ccgattacag tcgttcgcaa attcagctgt ggctgaaaaa tggagcggtt
    26581 tctgtcaatg gacagccagt caaagcaaat tacaaagtaa aacaaggaga cgagattact
    26641 gtcttgatcc ccgaaccaga aacattagat gtggtcccag aaaatatccc gttgacgatc
    26701 gtttacgaag atgaagatgt agcggtgata aacaaacctc aaggaatggt cgttcatcct
    26761 tcagcaggtc atacgcatgg cacaatggtc aatgcgctga tgtaccatat caaggatctt
    26821 tctacgatca acggcgttat tcgtccaggt attgttcacc gaatcgacaa agatacttcc
    26881 ggactgctaa tggttgccaa aaatgaccag gcacacgaat cattagctca gcaattaaaa
    26941 gataagacct ctttgcgcaa atatgttgct ttagtccatg gagagattcc tcacgaaaaa
    27001 ggaaccatca atgcgccaat cggccgttcg aaggaaaacc ggaaaatgca ggcgataaga
    27061 gaagatggga agtcggctgt cacacatttt actgtattgg aacgtttcga aggatttact
    27121 ctggttgaat taaaactaga aaccggaaga acacaccaga tccgtgtgca tatgaagtat
    27181 atcggttatc cgttagctgg agatccagta tacggaccga agaaaacttt gaaaggcaat
    27241 ggtcagtttc tccatgcgca aatgttaggt tttacgcacc cgactacaaa cgaaaagatg
    27301 atctttgaag cacctcttcc agaagttttt gaaaaaactt tagaaaaatt aagaaaaaac
    27361 attgcatttt aatttagata acggtatagt aattaacaac gaaagcataa aacttaatag
    27421 aattccttta agtttagtcc cgtgaggcta agaagggtgg gagcaaggat acgtatggtg
    27481 cgccataagt agattgctca ttgacatagt atagctatca gtgtatcagt atacgctcag
    27541 tctaaacatt cctcctgtcc tcttagggca ggagtttttt tatgcaaaaa agtaggggga
    27601 atgaaaatgc aagcaaaaga agtagtagat caggtaacca tgaagcgagc attgactcga
    27661 attacttatg agattatcga gagaaaccat agtatccaag atatcgtatt ggtaggtatc
    27721 aaaacacgag gaatctacat tgcatcaaga attgccgaac gcctgaagca gctagaagat
    27781 atcgatattc ctgtaggaga actggatatc actttatacc gagatgacaa aaaagaaaat
    27841 ccagaagaac cggaacttca ttcttctgac atcccagttt ccttagaagg aaaagaagtt
    27901 attttgattg atgatgtttt gtatacagga cgaacgattc gagcagcaat ggacgcagta
    27961 atggattttg gcagaccaag aaaaatttca ttagcagtcc tggtcgatcg tgggcatcga
    28021 gaattgccaa tccgtgcaga ttatgttggg aagaacattc caacatctcg ggcggaagag
    28081 attttagtag agatgcaaga gcttgatgga caagatcgca tcatgatttt gaaagaggaa
    28141 gattagagga gagagaaaca tgtcggagaa aaaaggattt cataatgatg acgtggtgtt
    28201 agatatccac gatcgcccaa ccccaggaca ttgggtagga ttaagtttac aacacttgtt
    28261 cacgatgttc ggagcaaccg tgttagtccc catcttagta ggtatcgatc caggtattgc
    28321 gctagtcagt tcaggactag gtacattggt gtacttattt actactaaag gaaagatacc
    28381 tgcgtactta ggaagcagct tcgctttcat tgcagcgatg cagcttttga tgaaaacaga
    28441 tggttatcca gcgattgcac aaggagcagt cacaactgga ttagtctatt tgattgttgc
    28501 gctgatcatc aaaaagatcg gttcagcatg gctggataaa atcttgcctc caatcgtcgt
    28561 cggtccagtc atcatggtta tcgggttggg acttgcggca aatgcagcaa caaatgctat
    28621 gtataatcag ggcacgtatg atttcaagta tattgctgta gcgctgatca cattaggatt
    28681 aacaattttc ttcaacatgt ggctaagagg ttttctcgga ttgattccga ttttactagg
    28741 tattattagc ggatacatcg tagccttgct gtttggaatc gtggatacta agccaatcgt
    28801 agacgctgcc tggtttgctg ttcctaactt tgagattccc tttgtacaat atgaaccaaa
    28861 gctatatctt ggagcaatca caacaatggc acccatcgct tttgtgacga tgacagagca
    28921 tatcggccat cttatggtat tgaataaact aactaaacgt aatttctttg aagaaccggg
    28981 tcttcataag acgttgacag gtgatggatt agcacaaatc gtggcagcct ttgtcggagg
    29041 tcctcctgta acaagttacg gtgaaaacat tggtgttctg gctatcacac gtgtccatag
    29101 cgtgtttgtg atcggtggag ctgcagtatt tgcagtcatt ttaggattcg taggaaaatt
    29161 aagcgctttg attttaagta tccctggacc agtcatctca ggaatcagtt ttatcttatt
    29221 tggagtaatt gccgcaagtg gaatgaagat tttaatcgag aacaaaatcg attttgataa
    29281 aaagaaaaat ctgttgattg cttcagttat cttagtagta ggaattggcg gactcgtctt
    29341 tgaagtaggc acatttacat tgtcggcaat ggcattagca actgtacttg gcatcatttt
    29401 aaatcttatc ttaccagaaa cttcacgaag cgaagaaaac taaggaggaa acattatgat
    29461 catcaaatca gaacgtatca gcttaaagca tttattgacc gttgaggcac ttactgatca
    29521 agaagttatg ggcttgatcc gcagagggca ggaattcaag caaggtgcta attggactcc
    29581 gcaaaagcgc cagtactttg ctacaaacct ctttttcgaa aatagtactc gcacacataa
    29641 aagcttcgat gtagcggaga aaaaactagg gctagaagtg attgaatttg aagcaagcac
    29701 aagctctgtc caaaaagggg aaacactata cgatacagta ctgacgatgt cagcattagg
    29761 cgtagacgta gcggttatcc gtcatggtga tgagaactat tatgacgaac tgatacaaag
    29821 taaaacaatc cagtgttcga ttatcaatgg aggagacgga agcggacagc atccaaccca
    29881 atgcttgctt gatttgatga ccatttatga agaatttggt cattttgaag gtctgaaagt
    29941 tgccatcgta ggtgatatca ctcactctcg cgtagctaaa tccaatatgc agatgttaaa
    30001 acgtctaggc gcacaagtat tcttctcagg tcctagagaa tggtacgacg atgaattcga
    30061 agtgtacgga cattatatgc cattggatga actactagat caagttgatg tcatgatgat
    30121 gcttcgggtg caacacgaac gccatgatgg aaaagaaagt ttctcaaaag aaggctacca
    30181 tcaggaacat gggttaacag ttgagcgtgc gaagaagttg caaaaacatg caatcattat
    30241 gcacccagca ccagttaacc gtgatgtaga attagctgat tcattagtgg aaggattaca
    30301 gtcgcggatc gttcagcaaa tgagcaatgg agtattcgtc agaatggcaa tcttagaagc
    30361 agtgctaaat ggaaaagcgt aattatagga ggatgaaaaa atgaaaacac tcattaaaaa
    30421 cggtcagatc aatacacgaa aaaatatgac gacaccagca gaaatttgga tagaagatgg
    30481 gcgaatcaaa gcaatcggta caggattctc agaagccgaa tttgatgaag tgtttgatgc
    30541 aaagggacaa ttgatcacac ctggtttagt ggacgtccat gttcatttga gagagccagg
    30601 atttacatac aaggagacga tcgaagctgg gactcgctca gctgctcgtg gcggtttcac
    30661 tactgtatgc gcgatgccga atttaaatcc tgtacctgat acggcagaaa agctgagaca
    30721 agtatatgac atcattagaa aagatgcagt tgtgaaggta ttgcagtatg cgccaatcac
    30781 agaaaatctg cgaagtgaga aattagttga tcaagaagca ctgattgaag aaggtgcttt
    30841 tgctttcaca aatgacggtg taggtgtcca aacagctggg acgatgtatc tggctatgaa
    30901 agaagctgca aaaaacaata aagccctagt agcccataca gaagatgaat ctttattatt
    30961 tggcggcgtt atgcacgctg gcaagaaggc agaagaactt ggtcttcccg ggatcttaag
    31021 cgtaaccgag tcttctcaaa ttgctagaga tctgttgcta gctgaagcaa ctggtgttca
    31081 ttaccatgtt tgccatgttt ctactaaaga aagtgtccgt gtaatcagag acgcaaaaaa
    31141 agcaggaatc cacgtaacag cagaagtttc accccatcat cttattttga tcgacgaaga
    31201 tattcccgaa gatttcggct tttggaaaat gaacccgccg ctaagaggac gggaggacag
    31261 agaagctttg atcgaaggct tgcttgacgg tacgatcgat tgcatcgcta cagaccatgc
    31321 cccccatgga ttagaagaaa aaagccaaag tttcatgaaa tcaccatttg gtatcgtagg
    31381 aagcgaaaca gcgtttcaac tgatctatac tcattttgta gaaactggtc gattcacgtt
    31441 ggaacaagtg atcaattggt tggcagttaa accggctgag atttttggtt tgaatgcagg
    31501 aacgctaacg gttggtgccc ccgctgatgt agcagttttt gatattacac aaacttgcac
    31561 gatcgataaa gaagactttt tatcaaaagg ggagaataca ccttttatcg gctggaaagt
    31621 aaaaggcgaa acacaaatga cttttgtcaa cggaaaactt gtgtggcaga agggagaata
    31681 aaagtatgga acgtttgctt atattagaag atggaacagt tttcgaagga aaggcttttg
    31741 gtgcagatgc tagtgtcttc ggagaaatcg tttttacaac aagtatgact ggttatcaag
    31801 aaactatcac tgatcaaagt tttaacggtc aaatcattac atttacctat cctatggtgg
    31861 gtaattatgg ggtaaataga gatgattatg aatcgatttc tccaacatgc aaaggagtag
    31921 tagtaaaaga acatgcacgt gtagcttcaa actggcgaaa ccaaatgacg ctagattcat
    31981 ttttgaaaca aaaaggaatt cctggaatat caggtatcga tacgcgcgcc cttactcgca
    32041 aaatccgtga agtcggaacg atgaaagcta gtatagttga tgctggagat tccttcgaac
    32101 atgcatttga tcagctgaaa gcaaccgttc tagcaaccaa tcaagtgcag caagtctcaa
    32161 ctaacaaacc ttatccaagt ccaggaacag gtcggaaagt cattgtcatt gacttcggct
    32221 tgaaacatag tattttacga gaattgagta aaagaaactg taacctcaca gtacttccct
    32281 ataacacaga tgccgagacg attttagcgc tatcaccaga cggagtcatg ctgacgaatg
    32341 gacctggtga tccaaaagat gtgccagaag caatcgaaat gatcaaaaaa atccaaggaa
    32401 aagtaccgat ttttggtatt tgtctcggtc accagttatt tgctttagcc aacggtgcag
    32461 atacctataa aatgaaattc ggacatcgag gattaaatca cccggtacga gaagtagcga
    32521 ccggaagaat tgattttact tctcaaaatc atggctatgc agtcgatgaa gccacgattg
    32581 atccagaaaa actcatcgta actcatgtag aagtaaatga cggaacagta gaaggagtca
    32641 aacacagaga ttatcccgct ttcagtgtgc agtttcatcc agatgctgcc cctggtccgc
    32701 atgatgcact acatttattt gatgaattta tggaaatgat ggacgcagga aaggagcagt
    32761 aaacagatgc cgaaacgtac agatatcaag aaaattatgg tgattggatc tggtccgatc
    32821 atcattggtc aagccgcaga atttgactac gcaggaacac aagcctgcct tgccttaaaa
    32881 gaagaaggat atgaagtggt tttagtaaat tctaatcctg cgacgatcat gacggataaa
    32941 gaagtggcag atcaagtata tatcgaaccg atcacacttg aatttgtatc aaggattctt
    33001 cgaaaagagc gtccagatgc tctccttcca actttaggcg gacagacagg cctcaatatg
    33061 gcaatggagc tggcagcatc aggtatttta gaagaattga acatagaact tctaggaaca
    33121 aagctttctg cgatcgatca agcagaagat cgtgatctat tcaaacagtt gatgaaagaa
    33181 ttgaatcagc caatccccga gtctgaaatc gtcaatacag tagaagaagc tgtagatttt
    33241 gcaaataaaa tcggctatcc tgtcatcgtt cgtccagcct ttactttagg cggaactggt
    33301 ggaggtatgt gtgaaaatga agaagaatta cgtatcgtat cagaaaatgg attaagtctt
    33361 tcccccgcta cccaatgttt gattgaacga agtattgcag gttttaaaga aattgaatat
    33421 gaagtgatga gagattcggc tgataatgca atcgttgtct gcaatatgga aaattttgac
    33481 ccggtgggta tccatacagg tgattctatc gtatttgccc ctagtcaaac attgtctgac
    33541 catgaatatc aaatgcttcg tgatgcttct ctttcgatta ttcgagcact taagatcgag
    33601 ggaggctgta atgtccaact cgctcttgat ccacatagtt ttaattatta tgtgattgaa
    33661 gtcaatccgc gtgtttctcg ttcatctgcg ttagcaagta aggcgactgg ctatccgatc
    33721 gctaagctag ctgctaaaat agcgcttgga ttaacattag acgaaatgaa aaatcctgtt
    33781 acaggaacga cttttgcaga atttgaacct gcattagatt atgttgtagc taaaattcct
    33841 cgctggccgt ttgataagtt tgaaaacgga gaacggcttt taggcacgca gatgaaagca
    33901 actggtgaag tgatggctat cggtcggaat atcgaagagt ctttattgaa agctgtgcgt
    33961 tctttagaaa tcggtacaca ccatatcgaa cttcctgagt tggcagacat cacggacgat
    34021 gacctaatcc aaaaagtggt tcgagcacaa gatgatcgcc tgttttatgt agctgaagct
    34081 attagaagag ggtacaccat agaagaatta gcggatttga caaaaatcga tttattcttt
    34141 ttagataaac tgttgcatat cgtagaaatc gaacagcagt tgaaacagca gccgttggat
    34201 gacaagatat tgaaaacagc gaaacaaaac ggttttacag acaaaaaagt ggcggagctg
    34261 tgggaaataa acgaagaagc cgttcgccag atgagaaaag aaaatgattt gattcctgta
    34321 tataagatgg ttgacacttg tgcagcggaa ttcgaatcaa aaacacctta tttttatagt
    34381 tcctatgaat atgaaaatga aagttcacga tcagaaaagc cctcaattct agtactggga
    34441 tctggtccta tccgtatcgg acaaggggtt gaatttgact atgcgactgt gcattctgtc
    34501 aaagcaatcc agcaggcagg atacgaagca atcatcatga acagcaatcc tgaaacagtt
    34561 tcgactgact tttctatttc ggataaatta tattttgagc cattgacctt cgaagatgtg
    34621 atgaatgtga tcgagctaga acagccgata ggagtaatcg ttcaatttgg tggtcagaca
    34681 gcaatcaact tagccgaacc tttgactgct gctggtgtaa aaatcttagg gacaacgatc
    34741 gaagacttgg atcgtgcgga aaatcgtgat ttattcgagc aagcgctgaa agagttggat
    34801 attcctcaac ctttagggga tactgctaca agtaaagaag aagccgtggt tatcgcttcg
    34861 agaattggtt atccagttct tgttcgtcca agctatgtat taggcggacg tgcgatggaa
    34921 atcgttgaaa accagcgaga tttagaagat tacatggaac atgctgtgaa agcatctcca
    34981 gaacatccag tactagtgga ccgttacttg cttggtggtg aatgcgaagt ggatgcgatt
    35041 tgtgacggtg aaacagtact catcccaggg atcatggagc atattgagcg tgctggtgtc
    35101 cattcagggg attcaatggc tgtgtatcca cctcaaacat tatctgaatc gatcaagaaa
    35161 acgatcgaag attacacgat ccgcttagca ttaggtttga attgtgtcgg gatgatgaat
    35221 atccagtttg tcatccatga aaatgaggtt tatgtgatcg aagtcaatcc tcgagcaagt
    35281 cggactgttc cattcttaag taaggtgaca ggaattccaa tggcacaaat tgcgacgaaa
    35341 gctattttgg gagagaaact aaagacactt ggttatgaaa acggacttta tccagaaaca
    35401 aaagaagttc atgttaaagc acctgtcttt tcttttacga aattgcagaa agttgatact
    35461 tacttagggc ctgaaatgaa atcgactgga gaagtaatgg gatctgacag acaattagac
    35521 aaagctttgt acaaggcatt cgaagcttct ggattacact tgcctgaata tggagccgta
    35581 ttatttacga ttgcggacga aacaaaagaa gaagcactag aattagctaa acgttttaca
    35641 gaaattggct atagtctgat aggaacgaaa catacagctg cttatttcga aaaaaatggt
    35701 ctggtcgttg aaacagtagc gaaaatttct gaggaagcag cagaaaaaaa tgtagtcgac
    35761 ttaataagag atggtaaaac acaagtggtt gtcaatacga ttgataaaga tcggggaaat
    35821 gcttctaaag acggtttcat catccgaaga gaagcagttg aacatggtat tccattgttt
    35881 acttctttag acacagcaga tgcgatcgtt cgagtgatgg aatcccgtgc tttttcaact
    35941 aaccctattt aacggcgagc acatgttaag acaaaaagac gaaaaaccgt tgcaggaggg
    36001 gacagatgaa acaagaaatg atggaaatcg ttcagcaaaa acagctggca ccaaagattt
    36061 ttgaaatgac attgaaacct gaataattca tagctttaat tctttggcac attttattga
    36121 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
    36181 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
    36241 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactggctc
    36301 gcctcaggcg agcaaagacc ctgtagaatt cattctgata cacatgataa ctagataatt
    36361 tgagatagac tcgtctaccc gtttggacta atttcccagc aactttcaga aacttcaatc
    36421 gaatagaatg aatggtcatt cccttttgga cttcttcaaa gccaatcgtt cttaagaagt
    36481 tgactaagtt gtaagctatc aagctgagca tcattcggac atgattctcc aaaaagcgag
    36541 gactgtctgt tttgtcaaag taaaagccag ctttcgcttc tttaatgaag ttctccattg
    36601 tgccacgttt ggcatagata gaaaagatgg tatcaggaga aacattttct gatagattcg
    36661 tcacgataaa ttcatgtcga aagagtagtt cgcccgcttc acgtgttgag cgtatacaca
    36721 cgcgacgact ttgtgaccag gattgtgctt gataagtggt cgagaagtac tgaacttctc
    36781 gttcttccca cttttgatta tcgccataaa gtactgattt ctcagctatt tgacctagtc
    36841 tacgattatt cttcagtcga ataacataat gactcttttt tgattcacac gaatcataca
    36901 caccaggtgt agcgaacccg ctgtctccac gaaccaagat gtcagtgttt ggtagagagt
    36961 gattatagtg ctctaataaa ggtgtgagaa actcctttac gccttttgat gtatattgat
    37021 ttcctgaacg tagttcagct tttaagaagt caccagtcaa tccgtcaaaa gctaccaatg
    37081 gatggtaacc ataggtttgg tagtgtgtat tataattcgt ttgttcttgg tgaccaaatg
    37141 tatctgaatg ggtcgaatct aaatcaatga ttaattccgt atcattacgg atgaggcgtg
    37201 ctttatcgat aagttcttgg ttcaaagctt gaagttcatg gatattctcc tcagatagtc
    37261 gatctaaaaa tcgggaaagt gaagattgag aagcgagttc tttcctatct aaaacagctt
    37321 taaacacagg atcttgtctc aggagattag ctgcagaatc agctgagtaa ccagcaatta
    37381 attgcataat gaactgctca agtatggata agttgtcatg agtaaaatac gctcgttcgt
    37441 cttcaatgtg aatgtgttgc ttagccagtt cagaaaaacc aaagatgttc atcagctctt
    37501 tcactaggac gagacccgaa tcactcgata attgaccacc tgtatgagaa atagtgatat
    37561 ttgaattgaa ttttacttgg tttttgtgta agctagtcat gaaaagaact cctttctttt
    37621 ggttggttta gtcactttaa ccatagcaga aaggggttct tttttcatca cttaacgggt
    37681 gaagatgaaa aagtaattgt aagctgccgt gaggcagttt cacatggttt taatt
//
LOCUS       DACCIQ010000021        36427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCIQ010000021 DACCIQ010000000
VERSION     DACCIQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.021"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            53..517
                     /locus_tag="IVM62_001053"
     CDS             53..517
                     /locus_tag="IVM62_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845024.1"
                     /translation="MELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQI
                     DDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHR
                     FIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            576..1016
                     /locus_tag="IVM62_001054"
     CDS             576..1016
                     /locus_tag="IVM62_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845025.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1028..1696
                     /locus_tag="IVM62_001055"
     CDS             1028..1696
                     /locus_tag="IVM62_001055"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6845026.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1760..2383
                     /locus_tag="IVM62_001056"
     CDS             1760..2383
                     /locus_tag="IVM62_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6845027.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2439..2825
                     /locus_tag="IVM62_001057"
     CDS             2439..2825
                     /locus_tag="IVM62_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP6845028.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3038..4429
                     /locus_tag="IVM62_001058"
     CDS             3038..4429
                     /locus_tag="IVM62_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP6845029.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5040..5408
                     /locus_tag="IVM62_001059"
     CDS             5040..5408
                     /locus_tag="IVM62_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6845030.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5588..6529
                     /locus_tag="IVM62_001060"
     CDS             5588..6529
                     /locus_tag="IVM62_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP6845031.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6598..7329
                     /locus_tag="IVM62_001061"
     CDS             6598..7329
                     /locus_tag="IVM62_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP6845032.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7473..9653
                     /locus_tag="IVM62_001062"
     CDS             7473..9653
                     /locus_tag="IVM62_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP6845033.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTELIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9673..10155
                     /locus_tag="IVM62_001063"
     CDS             9673..10155
                     /locus_tag="IVM62_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP6845034.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10171..10932
                     /locus_tag="IVM62_001064"
     CDS             10171..10932
                     /locus_tag="IVM62_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6845035.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11063..12397
                     /locus_tag="IVM62_001065"
     CDS             11063..12397
                     /locus_tag="IVM62_001065"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP6845036.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12704..13189
                     /locus_tag="IVM62_001066"
     CDS             12704..13189
                     /locus_tag="IVM62_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP6845037.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13182..13865
                     /locus_tag="IVM62_001067"
     CDS             13182..13865
                     /locus_tag="IVM62_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP6845038.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13991..16636
                     /gene="polA"
                     /locus_tag="IVM62_001068"
     CDS             13991..16636
                     /gene="polA"
                     /locus_tag="IVM62_001068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP6845039.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16681..17517
                     /gene="mutM"
                     /locus_tag="IVM62_001069"
     CDS             16681..17517
                     /gene="mutM"
                     /locus_tag="IVM62_001069"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP6845040.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17481..18110
                     /locus_tag="IVM62_001070"
     CDS             17481..18110
                     /locus_tag="IVM62_001070"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP6845041.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18162..18494
                     /locus_tag="IVM62_001071"
     CDS             18162..18494
                     /locus_tag="IVM62_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6845042.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18806..19297
                     /gene="nrdR"
                     /locus_tag="IVM62_001072"
     CDS             18806..19297
                     /gene="nrdR"
                     /locus_tag="IVM62_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP6845043.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19320..20693
                     /locus_tag="IVM62_001073"
     CDS             19320..20693
                     /locus_tag="IVM62_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP6845044.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20693..21619
                     /gene="dnaI"
                     /locus_tag="IVM62_001074"
     CDS             20693..21619
                     /gene="dnaI"
                     /locus_tag="IVM62_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP6845045.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21748..22188
                     /locus_tag="IVM62_001075"
     CDS             21748..22188
                     /locus_tag="IVM62_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6845046.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22332..23033
                     /locus_tag="IVM62_001076"
     CDS             22332..23033
                     /locus_tag="IVM62_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP6845047.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23030..24151
                     /locus_tag="IVM62_001077"
     CDS             23030..24151
                     /locus_tag="IVM62_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP6845048.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24166..25398
                     /gene="pepT"
                     /locus_tag="IVM62_001078"
     CDS             24166..25398
                     /gene="pepT"
                     /locus_tag="IVM62_001078"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP6845049.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25530..26438
                     /locus_tag="IVM62_001079"
     CDS             25530..26438
                     /locus_tag="IVM62_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP6845050.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26473..26745
                     /locus_tag="IVM62_001080"
     CDS             26473..26745
                     /locus_tag="IVM62_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP6845051.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26756..27802
                     /locus_tag="IVM62_001081"
     CDS             26756..27802
                     /locus_tag="IVM62_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP6845052.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28053..30662
                     /gene="clpB"
                     /locus_tag="IVM62_001082"
     CDS             28053..30662
                     /gene="clpB"
                     /locus_tag="IVM62_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP6845053.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30813..31484)
                     /locus_tag="IVM62_001083"
     CDS             complement(30813..31484)
                     /locus_tag="IVM62_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845054.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(31477..31971)
                     /locus_tag="IVM62_001084"
     CDS             complement(31477..31971)
                     /locus_tag="IVM62_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6845055.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31991..33211)
                     /locus_tag="IVM62_001085"
     CDS             complement(31991..33211)
                     /locus_tag="IVM62_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845056.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33358..35193
                     /gene="lepA"
                     /locus_tag="IVM62_001086"
     CDS             33358..35193
                     /gene="lepA"
                     /locus_tag="IVM62_001086"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP6845057.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35287..36414)
                     /locus_tag="IVM62_001087"
     CDS             complement(35287..36414)
                     /locus_tag="IVM62_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6845058.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12125 a         6454 c         7784 g        10064 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtatcgagct
       61 ttctcttgaa tcttgggatc gagcagtggt tgaactgttt ccagaaggtt taatgatcga
      121 tacaaacgat gggaagatcc atctgactgc agcagaactg caaaaacaaa tgcgttatca
      181 aatagatgac gaaaattctt tttctatttt tagacgtcag caaaagattc taaaaagctt
      241 tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg acacagctgt tgaaaaaata
      301 tccaggtctg cttcacacgt ctattcaatt tcctcaattg attacgatga tccatcgttt
      361 tatacgtatg caacagctac catctaagaa gctgtttctt ccattgacgg acacatttcg
      421 agtagtcaac agagaagata aaaagaaagt gattgtgatt gactttacca gaaatcggaa
      481 tgtccttcac caagttgcaa tggaaaaggc caattaaggc tttttctttt tggatttttt
      541 tgtcataata aatattagat gatggaggag ataatatgat gcaaacatgg ttagaaaggc
      601 taatacgatt tcagcagaac tatcacaaac ttatgcaggg aagatatgcg aaatttgatc
      661 agttgaatcg cggtctattg atcgctgcgc ttgtctttag tttgctaagt cgttggttgc
      721 cctatagaat cggacaattg ttgttcatcg tctttttcgg atggatgata tttcgtttcc
      781 tatcaaaaaa aatctacccc agactaaatg agaatcaacg ttatttgaaa tatcttgata
      841 gagtcaaaga aaaatatcat cagataaaaa acaaaaagca agtcaaaatg gatcgaaaaa
      901 gttatacttt ttttgagtgc ccgaattgtc atcaaaagca gcgtgcacct aaaggaaaag
      961 gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt cgaaacgaat gtttaaggga
     1021 gaaaagaatg aaattagtat tattacgtca tggcgaaagc gaagcaaatt ttgaaaatta
     1081 ctggacagga tggctagatg tcgccttaac agaaaaagga aaagaacaag cagcagttgc
     1141 cggtcaaaaa atgcttgaag ccggacttca attcgatgcc gtttatacct ctgttttgaa
     1201 acgtgcgatt cttaccggac aagtcgcact agaagaaatg ggtcagttat ggcttcctat
     1261 aataaaaaca tggcgtctaa atgaacggca ttatggggat cttgtgggta aaaacaaaga
     1321 taagatgaaa gaaatatacg gaaaagatca agtaaaaaca tggcgcagag gatatacgga
     1381 agttccgccg ttaaccgcag aaaatagatt tgaccgcaga tatgatcact tggatccgca
     1441 tttgatccca tacggagaaa gtctggaaat gacagtgaat cggatcattc ccttatggca
     1501 agatcatcta gcaccaaaac taaaagatcg gcaagattta ctagttgtgg gtcatggtaa
     1561 tagcatccga gcactgacaa aatatttaga agatgttcct gaagatcaaa tggatacgat
     1621 cgatatccca aatgcacaac caatccagta tacattagca gatgatttga caattctgga
     1681 caaaaaaata ctttaaagaa cacctttatc taaaagtgag agatagaaaa aagaagcaaa
     1741 ggaatggaac aaaaggaaca tgaaaaatta tatatgggac tttgatggta cattgtttga
     1801 tacgtatccc gcaatggtag atggtgcatg gcaagcacta aaagattttg gtatcagtat
     1861 ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct actagttatt tgatcaatga
     1921 atcaaacttg aatgccagag agtttaatga attatttcac cgatatgaaa aagaaagtac
     1981 ggaagtttcc cgtccatttc cagaaacgaa acaagtactg gaaatgctaa aagacaacgg
     2041 cggtcgtcat ttcatcctga cccaccgttt gacagaatca acatggggcc tgttaaaaga
     2101 acatcgattg gcacatctga ttgaggaagt agtagggatc gatcaggact ttcctcgtaa
     2161 acctgatcca gcatctttaa attatttgat cgatactttt catttagaaa gaacagacac
     2221 gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg aaaaatgcag gagtcgctac
     2281 ttgtctttac gacatcgatc attttctggg tgagatcccg gcagattatg tggtaggaaa
     2341 tctgaatgaa atccttacct tgcagagcat cattaaaaag tagtatcccc tttcaaagga
     2401 gatatgttat aattttcata aaggagtgat aaaattatat ggaaatcaga agacaaaaag
     2461 aagttgtaga agcatttcat tatgatatga gattgccgaa ccaagaagta gaaacagatt
     2521 tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa ctatccgaaa gaacatagca
     2581 tcgtaggagc acgcttagag ttccgtttag tgtttgatac atatgtttta tctggaagtg
     2641 tgagtcagat caatcatatc ctcaatcgga aaattgaagg tcaaaatgat gtcacacaag
     2701 aagaagtgga cgagctagta gcaccgttat tcgatatcgt acgccgaatg tcttatgagg
     2761 tgacagagat cgccttagat cagcctgggg taaaattgaa ttttcaatca gaaaaacaag
     2821 aatagacagt aaaaacatac ctgcgataga ttaaagctat cacaggtatg tttttttatt
     2881 actaattgta agcaaaaaga atgtcatttc gataaaaatc gtctaaaata taaaagtcac
     2941 tgaaaaaaat tttttcagga ggatggttga agaatcattc tcgggaacct ttacttcctt
     3001 cgatggactt gtcattaata atatgaggag gagaaaaatg acaacactca tcacagtttt
     3061 agtggttcta gtttttgcgg cattattata tgtgttctat cgtatgcaaa agcgccatac
     3121 aaaattttca acacgcgtct ttacagcatt aggagttggt atcgtctttg gcggtctgct
     3181 tcaattgatc tttggtgtag acgacaaagt cgttactcaa tcaatggaat ggatcgggat
     3241 tgtaggtcaa ggatatgtat cattcttgca aatgctagtt atgcctttag tgtttatttc
     3301 gatcattggt gcattcacaa aaatgaaagt ttctgaaaag ctagggaaaa ttagtgcgac
     3361 tgtattgact tctttacttg gaacaacagc tattgcagca ttgatcggaa ttggttcaac
     3421 aatgctgttc ggactccaag gagctaaatt cacacaagga gctgcagaaa cagcacgaat
     3481 ctctgaactg gcaacacgtc aggaaacagt tgaaaactta tcgattccac agcagatttt
     3541 atcctttatc cctaaaaacg tttttgctga tttagcaggt agtcgtgcaa caagtacgat
     3601 tggagtcgtt attttcgcgg catttgtcgg aatcgcttat ctaggcgtga aaagaaaagc
     3661 gccaaaagaa gcagcattct tttcagattt agtcgacagt ctgtataaaa tcattatgcg
     3721 gatcgtgacg cttgttttgc gtttgacacc ttatggagta tttgctttga tgacaaacgt
     3781 tttggcaaca agtgattttg cagctatcgt caatttaggt aaatttgtcg ttgcttctta
     3841 cgtagcattg cttgctgttt tgattgtcca ctcactgatt ttgattggtg caaaagtcaa
     3901 tccaatcaac tatttcaaaa aagcctttcc ggtattgagc tttgcgttta cttcacgttc
     3961 aagtgctgga gctttgccat tgaatatcga gactcagaca aaagctttag gagtagatga
     4021 tgcaacagct aatttctcag caagttttgg tttgtctatt ggacaaaacg gctgtgctgg
     4081 cgtttatcca gctatgttgg caacgatcgt cgcaccagca gctggagtga atgtctttag
     4141 tttggaattt attttgatgc ttgtggcaat tgtaacaatc agttcttttg gtgtagctgg
     4201 agtcggtggg ggcgcaacat ttgcttcact gatcgtttta ggggcaatga acttgccagt
     4261 agcaatcgtt gggttagtta tctctgttga accattaatc gatatggcac gtacagcagt
     4321 aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt gcacgcatca aagatttaga
     4381 tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg aatgcatagt caaaaagagg
     4441 ttgtgataga agtggtcagc tttaagaaat aagaagaagt ttcctaaaaa tagtttctca
     4501 aatttttgtg aaatttcggc ttatttccga gaagctactt ctgaaacacc gtgaataaga
     4561 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     4621 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     4681 aagaggtcgt gagcctgccg tttcactctg agtcacaaag agcaatttca aaaaatagct
     4741 tctcatgttt gttgaatatt gtgtcttgtg accgaggagt tgtcaggaga acaccgttta
     4801 ccagaggttg tgacttgttt ttagtcacga cctctttttt tatttattcc atataaatac
     4861 acattattta ttccgaaaag tgaaagcgca aataacaaaa acaaccaact tatttattat
     4921 taaagaaaag cgtatttaag taggacgatt ctgttgagag ctttcgtaga ttttcccatt
     4981 ttcctatggt aaactgacaa agttgagaat gttagaacag atcgtttagg aggaaacaga
     5041 tgtttacgaa tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt
     5101 tttggagagc agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa
     5161 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
     5221 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
     5281 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
     5341 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
     5401 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
     5461 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
     5521 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
     5581 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
     5641 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
     5701 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
     5761 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
     5821 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
     5881 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
     5941 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
     6001 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     6061 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     6121 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     6181 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     6241 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     6301 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     6361 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     6421 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     6481 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     6541 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     6601 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     6661 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     6721 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     6781 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     6841 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     6901 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     6961 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     7021 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     7081 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     7141 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     7201 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     7261 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     7321 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     7381 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     7441 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     7501 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     7561 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     7621 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     7681 tagaaaaacg taaaactgaa ttgattcggc taatcgatga acaagggaaa ctgactcccg
     7741 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     7801 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     7861 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     7921 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     7981 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     8041 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     8101 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     8161 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     8221 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     8281 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     8341 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     8401 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     8461 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     8521 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     8581 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     8641 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     8701 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     8761 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     8821 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     8881 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     8941 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     9001 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     9061 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     9121 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     9181 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     9241 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     9301 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     9361 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     9421 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     9481 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     9541 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     9601 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     9661 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     9721 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     9781 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     9841 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     9901 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     9961 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
    10021 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
    10081 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
    10141 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
    10201 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
    10261 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
    10321 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
    10381 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
    10441 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
    10501 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
    10561 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
    10621 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
    10681 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
    10741 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
    10801 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
    10861 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
    10921 tctcgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
    10981 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
    11041 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
    11101 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
    11161 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
    11221 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
    11281 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
    11341 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
    11401 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
    11461 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
    11521 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
    11581 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
    11641 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
    11701 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
    11761 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
    11821 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
    11881 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
    11941 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
    12001 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
    12061 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
    12121 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
    12181 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
    12241 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
    12301 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
    12361 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
    12421 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
    12481 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
    12541 gaagctgttt tttcttggct gttcttatta tttgatgcag gacagctttt tcacgacctc
    12601 tgtaaactat aggaatctgt taaatcaagg gagcagaact tgccttttac gctacttttg
    12661 gtacaataat gtatgattaa aaataaactg gagggtttct atcttgaaca taggtaagcc
    12721 gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat ccttaacaga
    12781 atctattgaa gataaccagc ttttgctgta tttgggattg acgaatgatg caaatccttt
    12841 gtatatccaa catgactttg cacaaaaaac agaatatcaa cagccaatcg ttccatcagt
    12901 gatgctgatg gggatcatca caagtgcaat ttcaaagcat cttcctggac caggatcaca
    12961 tgttgtcaat ttttctgtca attttattga accggttttc cattatgaaa cactgacttt
    13021 tcagtttgag gtcataaaag tagataagat gaaggatgtg gtgacgattt ctgtagaagc
    13081 ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac cgccacaagt
    13141 tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg aatgaataat ccaagtacat
    13201 caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg atttttaggt atgggacttg
    13261 cccttagtgc agtcacttct tacttggctc tttgggtatt tcctttgcaa ttagcaactt
    13321 ttatcaataa ttttccacta ggctttgtgg gtctgtggat cgttgaactg attttagtaa
    13381 tggttctagg cgtaaaagca caaaagaatc cgagcttagc aatcggtgga tttattgcct
    13441 actctctttt gaatggcata acattagcgg ttactcttgc atactacgat attggaacag
    13501 taacgcaagc tttcttgaca gctactttga tgttcatggg gatgtctgtt gtcggagtaa
    13561 caacaaaaag agatcttagc ggggttggaa gagcaggaca ggcagcttta atcggagtga
    13621 tcattgcact agtcttgaac atcttcattt tgagaagtag cgtagttgag ctgttcatct
    13681 ccctactgtt agtcgtcatc tttgcaggga ttacagctta tgataaccaa agcatccgta
    13741 atatctatgt ccagacaaac ggaacagcac atagtggaat tgctgtattc atggcattga
    13801 agctgtattt ggactttatc aacttgttct tggcgtttct gagaatcttt ggacggaaca
    13861 actaaaaaag aaaaaaattc atcagttggg gctataccga attatttggt atggtcccgc
    13921 tttttctttt cgcattccgc aggattcttt gttagaatga ataagaactt tacagtaagg
    13981 agttaagaag atgacaaaaa ataaattatt actcgttgat gggaacagtg tggcttttcg
    14041 cgcattcttt gcgcttcaca attctcttga acgtttcaaa aatagaaacg gtcttcatac
    14101 aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag agcaaccgac
    14161 tcatgttctg gttgcgttcg atgcaggaaa aacgactttt cggacagaaa tgtatgcgga
    14221 gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc catatatacg
    14281 tgatttgttg acaggactag gggtacagta ttacgaattg cccaattatg aagcagatga
    14341 tattatcggt actttggcag aaaaagtaga caaagatcaa tttgatgtcg tcgtgctttc
    14401 aggtgatcgg gatttgactc aattagctag taaagaagta aaagtggata tcactgtaaa
    14461 aggtgtcagc gatatcgaaa gctatacacc agaacatgta gcggaaaaat atgatgggtt
    14521 gacgccaaaa cagattattg acatgaaggg actagccgga gatgcctctg acaatatccc
    14581 cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat atggctcagt
    14641 cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag aaaatctgat
    14701 aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg accatcaata cttcagcgcc
    14761 tgtggatgtc tctatcgact cattgaagta tgaagggaaa aatctcgata aactcgttcc
    14821 gttttataaa gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg tagaagaaga
    14881 agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg aaatgtttac
    14941 tacggatatg gcgttgtacg tggaaatgat gggcgacaat tatcatacag aggaaatcgt
    15001 cggagtagcg tgggggacag aaaagaaaat ttatgtcacg aacgatctat ctgtattcga
    15061 aaaccgagcg ttccattctt ggatcactga tccgactcgg ctgaaaaaag tatatgatgc
    15121 gaaacgaact tatgttgctt taaaccgcta tactggaaaa acgaaaggaa tcgattttga
    15181 tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa agcacagata tcgaaggaat
    15241 agctgctcat tacggataca acgatatcca atcagatgaa gctgtatacg gcaaaggggc
    15301 gaaaaaaggt ttaccagaag aagaggaact cttttttgct catttagcgc gaaaagtagc
    15361 tgcaatcaat gcgttgacac caaaattgag ccaagaactg gcagaaaaaa accaagaaga
    15421 tcttttccgg aaaatggaac tgccattgtc tatcattttg gcagagatgg aaataagcgg
    15481 gatcaaagta gatgcgactc gcttacaaga aatgaagggc gagttctctg ctcgcttaag
    15541 agagatcgaa caaaaaatct acgaagaagc cggcgaagaa ttcaatttga attcaccaaa
    15601 gcaattagga gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa aaacaaaaac
    15661 tggatattcc acagccgtag atgtactgga acagcttcgt gaacaagcac caatcgtaga
    15721 ggatatcctc acctatcgtc aaattgcaaa aattcaatct acctatgtcg agggactgct
    15781 gaaagtgatc caatcagatg gaaaagtgca tacccgttat gtccagacat tgacccaaac
    15841 aggacgatta agctctgttg atcctaattt gcagaacatc ccgattcgat tagatgaagg
    15901 gagaaagata cgtcaggcat ttgttcctag agaaaaagac tggctgattt attcttctga
    15961 ctattcacaa atcgagttac gcgtacttgc gcatatctca aatgatgaac atttgaaagc
    16021 tgccttccta gaaggtcaag atatccatgc cagcacagcg atgcgtgtat ttggaatcga
    16081 aaaggcagag gatgtcacac caaatatgcg taggcaggca aaagcagtga attttggtat
    16141 cgtgtatggc atctctgatt acggactttc acaaaatcta ggaatcagcc gaaaagcagc
    16201 acagcaatac atcgatacgt actttgaaaa atacccaggt gtcaaagaat atatggaaag
    16261 aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc gccgtcgata
    16321 tctgccagac atcaattcaa gaaattacaa tatccgttct tttgcggaac gaacagctat
    16381 caatacacca atccaaggaa gtgctgctga tattttgaaa atcgcgatga tcgaattgga
    16441 taaacgatta aaagaaacag gtcttcaagc tacgatgctg ttacaagttc atgatgaact
    16501 tgtcttcgag gtaccggaaa aagaacttga atcattagat aaattggtga aggaagttat
    16561 ggaacaagct gtttctcttc atgtaccact gatcactgac agcagttggg gaaaaacgtg
    16621 gtacgaagca aaataaacat tgtttaagac agagcaatat aatgaaggtg aaaagaaaaa
    16681 atgccagaat taccagaagt agaaaccgtt cgtaaaggat tggaacggtt agtagtagga
    16741 aaaacgattc aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc agagacaccc
    16801 atctttgagg cttctcttgt tggcgaaacc atccaatcca ttggacgtag agggaagttt
    16861 ttaatttttc atctcgatca ttgtgaactg atctcacact tgcgaatgga aggaaaatat
    16921 caatttacga aagaaaatac accaatcgat aaacatacgc atgtactttt cttttttgag
    16981 gatggaagcc aacttcgcta caacgatgta cgaaaatttg gtcgtatgac aatcgtcgaa
    17041 aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg gacctgagcc tttgccagat
    17101 agcttcttat tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc aatcaagcca
    17161 ttgctgctag accaacgtct agtgactggt ctaggtaata tttatgtaga tgaagcatta
    17221 tgggaagcga agatccatcc agaacaaccg gcgaacacac tgcgccccaa agaggtagaa
    17281 caattacgtc tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg aggtaccacg
    17341 attcgcagtt atttgaatgc attaggagaa tcaggcggat ttcaagtagc actgcatgtc
    17401 tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa aacaaaagtg
    17461 gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc aaagtaggat
    17521 ttgttttagg tttgacgggt ggaatagcta ccggtaaaag tacggcagcc aaagtctttc
    17581 agtctcatgg atttcctttg atcgatggcg atgtgattgc cagagaaact gttgagcctg
    17641 gaacgcttgc tctgaggaaa attgtatcag tgtttggcca agaggtattg cagcctgatg
    17701 ggcaactaga tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa aaacggcaaa
    17761 aacttgatca gctattagat ccgtttatcc gaaaagcgat aaaagatcaa atcgcctctc
    17821 taagttccaa tcatccttta gtaattgtag atatcccatt gctctacgaa ggtcattatg
    17881 accactatat ggatgcagta gctgtcgttt atactacgcc agaaactcag cttcagcgtt
    17941 tgatgaaacg taatcaactg acgctagaac aagcgcagca gagaattagt agtcaacttc
    18001 caatcgaaga aaaaaagcag cgtgcggata ttttgttcga caacaatgga acaaaagaaa
    18061 aacttgtggg acaaatcgaa aattggcttt ttgctaatcg ctttctttga gtatctgtgt
    18121 caattttgct atcctgagta tatagaaagt gagggatagc gatgggatca ttaaaaaatc
    18181 aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc aataaagaaa
    18241 atctaatcga tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa aagcagtacc
    18301 aagcaagaat cgatgaaata gaagaccagc aggcagaaga agaagcgaag cggcaagatt
    18361 tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc ccagaaagtg
    18421 accacattcc cgatgagcaa aaaatcgagg aacagcgaat gaaacactat acaggaatcg
    18481 aagagaagtt ataaaatagt aaaaaatcga gatgtgactt gaagcagatg agaaggtgtt
    18541 catctgcttc aagttttttg tgctagatga ctaaatcagc ttttgtgtaa gttatttctc
    18601 agtaatcaag gtgatcagta ttggaaaatt gaaatttagt aaggacaggg gacacaatta
    18661 cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt ttgggtagga cagtcgcgtt
    18721 tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga aagacacatc ctttatataa
    18781 aatgcgatgc aagaggtgag acaaaatgag atgtcccaga tgtcaacata ataattcacg
    18841 agtaatcgac agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc gcgaatgtga
    18901 gaattgttcg ttccgattca ctacatttga acgaattgaa gctgctccgt tattagtaat
    18961 caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa attttaagag gactcatccg
    19021 ttcagcagaa aaaagacctg tagctatgga acaaatggaa caaatcgtca accatgtcga
    19081 aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg aatctaattg gtgaatatgt
    19141 gatggaagat ttagttgatt tagacgaaat cgcttatatt cgttttgcaa gtgtttatcg
    19201 ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg ataaagcaaa
    19261 agaaaataat aaagagaata ataaagaaaa caattaagaa acaaaaagga ggcatttcct
    19321 tggaaaatgc ttggaaagag cttcaaccaa aaaatattta ccaaggaaga aaagcggctc
    19381 cgctgagaga agacagcaag aacgccttgc tgtatcttta tcagccacta atcggcggag
    19441 aagccttgag tatctatttc accctgttat cagaaatctc tcaagaatct ggcaaaggtc
    19501 ctgaagggat gcatgcagat ctgcttagta gtctaggctg cggtatcccg caattttatg
    19561 atgcaagaaa aaagctagaa ggaatcggct tactggatgt ttattttaaa gaagatagtg
    19621 atctgggacc tcgttttatt tatgagctgt tagaaccact cattcctaaa gcatttttca
    19681 aagactctgt tctggctttt cttcttctgg aaaaagttgg tgaacgacga ttccagaagc
    19741 ttacccaatt ctttgaacct aaaaggcata cagcagaggg atatcaaaaa atcacaaaaa
    19801 aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa gaaaacatcg
    19861 aatcgctgaa taaatctttt gaagaatctg aatcagtagc attacttgaa gcagattcga
    19921 cattcgattg gcagtttttg aatgattggc tggcaaaaaa acatattgca agtcttgatg
    19981 atccaatcat caaacaagtc gacatgtatc atcagcttta cggtttcgat gaattgacac
    20041 ttggcgaact gatcagtcaa tcttatgact ttagtgaaca aaaagtatct gaaaaagaac
    20101 tgcaaaaagc cattttggca atgcagagac gtccggtgaa gcaaacaaaa gaaacagttg
    20161 cggtttctga aaacagtaat gaaccctcgt caaacgaaca tttgacaaac gcagcgcttc
    20221 agctgataaa agaagcacag agcattccac cgatgaaata tctggaagca atcaagtcag
    20281 aaaaaggcgg atatgtttct aaacaagaaa actggttact gcaagatctt gtttctcaat
    20341 caggtctttc cagtagcgtg atcaatgttt tgctgaatta tgtactagtg atcaagaata
    20401 atgctagctt aaatgccagt tatgtaaata cagtggcaaa tgaatgggca caaaagaaaa
    20461 tcgccacagc agaagaagcg atccagcaca ttcggcaaat aagtaatcaa gcaaaacaat
    20521 caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga cgggaaaagc
    20581 ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag aaaaaagcag
    20641 agattgaccg ccgttttaaa gaatacctgg caaaaaagga gggtgatatc tagtggaaga
    20701 cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat tatcaagaaa agtatgctga
    20761 aatgatcgct gaagtcttaa atgatgcgga tgttcaagct tttctaagcg cacaccaaga
    20821 agaattgaca caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat tcgtgcaaga
    20881 aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct cccggctatg agccgaagct
    20941 gacgttgaat tttcattatg tggatgtcac ttatgtacca acggaagcat tgttagcacg
    21001 gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg gatatgccaa aagatattca
    21061 atcggcttct tttgaaaact ttgaacgtac agatggacga ggatatgcga gcttagaagc
    21121 cctagatttt gtagagaaat atactgctga tccaaaaggg tatcataaag gactgtatct
    21181 agtaggaagt tttggtattg gaaaaacata tttattagga gcaacagcga gagaattagc
    21241 gattgctgga ttcaccacga cgctagtcca ttttccgaca tttgccgttg agatgaaaca
    21301 ggcaatcgga aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt cacctatttt
    21361 gatgatcgat gacattggtg cagatgcgat gagtagttgg atacgagacg aagtattcgg
    21421 tgttatcctt caatatcgga tgcaggaaca gttaccaacc ttcttctcat cgaattttac
    21481 aatggatgaa ttggaacagc atttgtccgt cactcaacgt ggagatgaag agccgctaaa
    21541 agcgaaacgg ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa tgactggtcg
    21601 caaccgacgc aatggttgaa cagttgacaa gtccaactga catttgacct agtagtaaaa
    21661 gcaggtcagc gaatcattat atggacagaa atgaccggag acaatcaccg caacaagtaa
    21721 tgcagaaact taaacggagg attttttatg agggctattt tttctagtat atctgtagtt
    21781 ggtctatttt ttcttttagc aggctgcggt agcgcagata gcaacacagg ttctgttgat
    21841 tcgattgatg cgggtcaaac agtaaaaaca agcagtacga aaacggagag acaaagcata
    21901 tttgtcggaa cgattgaaag tatcactcaa gtagaagcag aagaagaaac gcttcagttg
    21961 aaactcgtcg atgtcatggc caaagaagat ccagcgaata tgggaacgtc attcagcaat
    22021 gacggagtga tcttaaatgc tactgctgaa aaaataagcg tggacataaa tgaattgaaa
    22081 atcggtgaca aagtccaatt tacattagtt gagatgccga tcatgacgat gtctatccca
    22141 ccgcaagttc ctggagattc gattattgat gtttcattga acaaataacc aataacattt
    22201 tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat
    22261 gggaattttt ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa
    22321 ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg
    22381 tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat
    22441 tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg
    22501 aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg
    22561 caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta
    22621 tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg
    22681 gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc
    22741 aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg
    22801 aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt
    22861 tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc
    22921 ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc
    22981 aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag
    23041 cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg
    23101 agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac
    23161 attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat
    23221 tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga
    23281 aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat
    23341 ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga
    23401 gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt
    23461 ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga
    23521 ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc
    23581 acaaccagcg attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt
    23641 tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta
    23701 tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg
    23761 acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga
    23821 agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa
    23881 gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag
    23941 agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc
    24001 tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat
    24061 cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga
    24121 aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt
    24181 ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg
    24241 actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag
    24301 ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca
    24361 agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat
    24421 tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc
    24481 gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat
    24541 tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa
    24601 tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct
    24661 cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa
    24721 ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt
    24781 gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat
    24841 gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt
    24901 cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc
    24961 catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat
    25021 catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg
    25081 aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg
    25141 aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag
    25201 cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct
    25261 tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc
    25321 gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa
    25381 ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg
    25441 atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc
    25501 ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa
    25561 gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa
    25621 agcagccaga attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat
    25681 tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt ggagacggca
    25741 cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac
    25801 ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag
    25861 aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg
    25921 atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca
    25981 gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat
    26041 tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa
    26101 aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg
    26161 actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt
    26221 tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca
    26281 ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc
    26341 gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat
    26401 taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt
    26461 ggaagaagag taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg
    26521 aatttttact tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg
    26581 cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg
    26641 atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct
    26701 gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc
    26761 gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc
    26821 cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac
    26881 cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg
    26941 gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt
    27001 cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat
    27061 ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac
    27121 agcactccat atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt
    27181 catccaattc tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg
    27241 tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt
    27301 tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg
    27361 ggtggataca tgcaatcagc agatctactc tctaagggaa acggtccaaa ccattatcca
    27421 agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta
    27481 tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat
    27541 ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg
    27601 ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt
    27661 ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt
    27721 gtatgtgatt gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga
    27781 gaataaagaa aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga
    27841 aaaacgttat ggataagata attttacgtt tgtttggctc tttttttata tacatacaat
    27901 caattttgtt tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ctatgtgtat
    27961 attttgacca aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt
    28021 gatcttacct ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc
    28081 ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg
    28141 acattgccca tgtatggaaa atctttttac aatctaatca ttttggtcga aatttctata
    28201 cggatgccgg attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt
    28261 atccagttgt ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc
    28321 gattattgaa tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg
    28381 aagtcgttat tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata
    28441 aaaacggatt atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa
    28501 gagtgacctc acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt
    28561 tagtacagca agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc
    28621 gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac
    28681 ccggtgtagg gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg
    28741 tgcctgaaaa tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg
    28801 gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa
    28861 aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa
    28921 aaacagaagg aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat
    28981 tacatttgat cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag
    29041 cgttggaacg tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca
    29101 gtattctgcg tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca
    29161 acgcattagt agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg
    29221 acaaagcaat cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta
    29281 tgccaacgga attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg
    29341 cactgaaaaa agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat
    29401 tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag
    29461 aagtcaattc tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag
    29521 atgcagaaaa taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc
    29581 aattagaaaa agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg
    29641 ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc
    29701 ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc
    29761 ataaacgagt gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt
    29821 cacgggctgg attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa
    29881 ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag
    29941 atcatatggt acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag
    30001 tcggagctcc tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca
    30061 gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct
    30121 tcaatatctt gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag
    30181 tagatttcaa aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg
    30241 aaggcgtgac tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat
    30301 tacgtggaaa cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac
    30361 cattgagcct agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac
    30421 gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg
    30481 aaaatgctta tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag
    30541 tagaaacacc actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa
    30601 cgattacttt acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct
    30661 aatcttccat tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc
    30721 ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt
    30781 gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta
    30841 taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag
    30901 gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg
    30961 acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg
    31021 cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat
    31081 aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc
    31141 aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa
    31201 aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa
    31261 aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact
    31321 tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga
    31381 gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca
    31441 aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc
    31501 taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa
    31561 tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg
    31621 tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc
    31681 gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat
    31741 cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc
    31801 aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg
    31861 aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt
    31921 atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc
    31981 atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta
    32041 gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac
    32101 ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta
    32161 taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat
    32221 tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa
    32281 acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga
    32341 tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat
    32401 ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc
    32461 gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt
    32521 agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat
    32581 cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat
    32641 ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc
    32701 gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg
    32761 aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat
    32821 acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat
    32881 cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca
    32941 ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca
    33001 gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac
    33061 ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga
    33121 acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt
    33181 caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa
    33241 cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa
    33301 ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg
    33361 gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat
    33421 atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg
    33481 tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc
    33541 acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc
    33601 tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt
    33661 gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca
    33721 ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa
    33781 atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga
    33841 atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt
    33901 ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa
    33961 gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc
    34021 acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt
    34081 gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc
    34141 ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat
    34201 acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat
    34261 ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa
    34321 gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa
    34381 gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa
    34441 gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac
    34501 catgttaata aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa
    34561 cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat
    34621 gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg
    34681 gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc
    34741 tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa
    34801 atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa
    34861 gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc
    34921 gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct
    34981 attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct
    35041 aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg
    35101 aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt
    35161 ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa acagtgtaaa
    35221 aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtggttt ttttcggtgg
    35281 caaaaatcaa gaattctttc taagattttc catgatatcg accgtttcat ttctcatctt
    35341 atctgttaca tgcgagtaag tatccatcgt aattgaaatt ctgctgtggc ccaatcgttc
    35401 agagatttct ttcatttttg cgccattttc gagaagaagt gtagcatgag tatgtctgag
    35461 agaatggaag ttaaaagaga gggagagtgc attcgatatc ctcctagtat tccatttcac
    35521 tacacttggc gtaactagct caccgtcttc cttcgtacat actgcatttg aatcaatgta
    35581 gagctttcca tacttcattc gattttctaa ttgttgtttc ttatgttttt tcagaattgc
    35641 tagcaaggtt tgtccaataa aaatcgttcg attggaactg cttgtctttg gcgtaccata
    35701 tacccatgcg ccgtcattct ttaccatttg tttctctaca gtaattgttc catttgaaaa
    35761 atcgacatca tcccacgtca gaccacaaac ttcgccaacg cgcattcccg tataaaatcc
    35821 aatattcaaa ggaatataga aaggatgtcc ttcaggagta atttctagca tatgatcaaa
    35881 gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt ggtcgtgctt catattttgg
    35941 tatttttaca tacagcatag gattttgctt gattaatccc caaggataaa ccgccatatt
    36001 cagcgcattc ttaaggacag agtgagtaat agtcattgtt ttcttcgagt aacccttttt
    36061 aaattcagca ttgatgaaat tttgtaaaag agcaggggag agatccgtaa gttttttctt
    36121 tcctaaataa ccgtttatat gatttttgat ggtaaatcgg tagttttcat aggtattgta
    36181 ttttagattt agtttaacgt attcctccat ccaaaaatca aggtattgtt ttactcgagt
    36241 atccgtacct aaaaagtatt gtcctgtttc gtcaatatct gataaaactt ttcgtaaagc
    36301 agcttcggcc tctggtcggg tgtctccgcc aactttctcc actttttttc ttgagccatc
    36361 atcattgata tcttcgccgt ttgtataatt aagcgagaca aataaaaaag ccatttatgt
    36421 tacactc
//
LOCUS       DACCIQ010000022        35737 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCIQ010000022 DACCIQ010000000
VERSION     DACCIQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.022"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            71..1141
                     /locus_tag="IVM62_001088"
     CDS             71..1141
                     /locus_tag="IVM62_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845059.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1156..1824
                     /locus_tag="IVM62_001089"
     CDS             1156..1824
                     /locus_tag="IVM62_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845060.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1844..2422
                     /locus_tag="IVM62_001090"
     CDS             1844..2422
                     /locus_tag="IVM62_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6845061.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2823..3734
                     /locus_tag="IVM62_001091"
     CDS             2823..3734
                     /locus_tag="IVM62_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6845062.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4112..5029)
                     /locus_tag="IVM62_001092"
     CDS             complement(4112..5029)
                     /locus_tag="IVM62_001092"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP6845063.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5231..5668
                     /gene="zapA"
                     /locus_tag="IVM62_001093"
     CDS             5231..5668
                     /gene="zapA"
                     /locus_tag="IVM62_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP6845064.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5726..6274
                     /locus_tag="IVM62_001094"
     CDS             5726..6274
                     /locus_tag="IVM62_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP6845065.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6389..8749
                     /locus_tag="IVM62_001095"
     CDS             6389..8749
                     /locus_tag="IVM62_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6845066.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8836..9150
                     /gene="trxA"
                     /locus_tag="IVM62_001096"
     CDS             8836..9150
                     /gene="trxA"
                     /locus_tag="IVM62_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6845067.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9332..11110
                     /gene="uvrC"
                     /locus_tag="IVM62_001097"
     CDS             9332..11110
                     /gene="uvrC"
                     /locus_tag="IVM62_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP6845068.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11255..12154
                     /locus_tag="IVM62_001098"
     CDS             11255..12154
                     /locus_tag="IVM62_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845069.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12147..13409
                     /locus_tag="IVM62_001099"
     CDS             12147..13409
                     /locus_tag="IVM62_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845070.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13570..15468
                     /locus_tag="IVM62_001100"
     CDS             13570..15468
                     /locus_tag="IVM62_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP6845071.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15798..16517)
                     /locus_tag="IVM62_001101"
     CDS             complement(15798..16517)
                     /locus_tag="IVM62_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6845072.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            16854..17186
                     /locus_tag="IVM62_001102"
     CDS             16854..17186
                     /locus_tag="IVM62_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845073.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17173..19176
                     /locus_tag="IVM62_001103"
     CDS             17173..19176
                     /locus_tag="IVM62_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6845074.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19195..19665
                     /locus_tag="IVM62_001104"
     CDS             19195..19665
                     /locus_tag="IVM62_001104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6845075.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19686..20270
                     /locus_tag="IVM62_001105"
     CDS             19686..20270
                     /locus_tag="IVM62_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6845076.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20285..21271
                     /locus_tag="IVM62_001106"
     CDS             20285..21271
                     /locus_tag="IVM62_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP6845077.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21261..21572
                     /locus_tag="IVM62_001107"
     CDS             21261..21572
                     /locus_tag="IVM62_001107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP6845078.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21594..23375
                     /locus_tag="IVM62_001108"
     CDS             21594..23375
                     /locus_tag="IVM62_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP6845079.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23368..24744
                     /locus_tag="IVM62_001109"
     CDS             23368..24744
                     /locus_tag="IVM62_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP6845080.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24751..25386
                     /locus_tag="IVM62_001110"
     CDS             24751..25386
                     /locus_tag="IVM62_001110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP6845081.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25416..26771
                     /locus_tag="IVM62_001111"
     CDS             25416..26771
                     /locus_tag="IVM62_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP6845082.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26963..28357)
                     /locus_tag="IVM62_001112"
     CDS             complement(26963..28357)
                     /locus_tag="IVM62_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6845083.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28449..29420
                     /locus_tag="IVM62_001113"
     CDS             28449..29420
                     /locus_tag="IVM62_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP6845084.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29417..30199
                     /locus_tag="IVM62_001114"
     CDS             29417..30199
                     /locus_tag="IVM62_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP6845085.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30368..30739)
                     /locus_tag="IVM62_001115"
     CDS             complement(30368..30739)
                     /locus_tag="IVM62_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6845086.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30873..31316
                     /locus_tag="IVM62_001116"
     CDS             30873..31316
                     /locus_tag="IVM62_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6845087.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31359..32168)
                     /gene="yidA"
                     /locus_tag="IVM62_001117"
     CDS             complement(31359..32168)
                     /gene="yidA"
                     /locus_tag="IVM62_001117"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP6845088.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32187..33554)
                     /locus_tag="IVM62_001118"
     CDS             complement(32187..33554)
                     /locus_tag="IVM62_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6845089.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33538..34368)
                     /locus_tag="IVM62_001119"
     CDS             complement(33538..34368)
                     /locus_tag="IVM62_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP6845090.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34531..34968
                     /locus_tag="IVM62_001120"
     CDS             34531..34968
                     /locus_tag="IVM62_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP6845091.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35044..35664
                     /locus_tag="IVM62_001121"
     CDS             35044..35664
                     /locus_tag="IVM62_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP6845092.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11671 a         6062 c         7682 g        10322 t
ORIGIN      
        1 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
       61 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
      121 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
      181 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
      241 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
      301 agtcgaaggt gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga ctagtaaaga
      361 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
      421 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
      481 tagtttgaaa gaagaatatg gtctagccat aggagataca gttaagttat ctggtagtga
      541 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
      601 ctatacaacc atcaatgctt accaagaaat ccgcttcgaa aaagaagata caagtgaaaa
      661 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
      721 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
      781 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
      841 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
      901 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
      961 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
     1021 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgttagtag tagctgtgtt
     1081 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
     1141 agaaggagga agaacatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
     1201 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
     1261 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
     1321 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
     1381 gaaagcgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
     1441 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat taaacatgaa
     1501 catcgtcaaa aagaagcaaa cgaattattt gagcagcttg gcgtggataa gttgatcaac
     1561 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
     1621 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
     1681 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
     1741 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
     1801 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
     1861 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
     1921 tctcggcact tcaacaagag aaatcgcaca aaaagttggg atcacccagc ccaatctcta
     1981 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
     2041 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
     2101 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
     2161 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
     2221 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
     2281 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
     2341 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
     2401 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaatagttag gtgaagaagc
     2461 aagatatgac gaagatcgtt atatcttgct tctttatata ggtagttttt cataaataca
     2521 aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag
     2581 gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta
     2641 ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa
     2701 aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt
     2761 gcttacttaa gctgatttta agttttggcc cttagtctgg atctacgagg tgagagtcaa
     2821 gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc
     2881 aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta
     2941 cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg
     3001 gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa
     3061 acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa
     3121 atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac
     3181 aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa
     3241 atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta
     3301 cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata
     3361 ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag
     3421 atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca
     3481 cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca
     3541 ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa
     3601 atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg
     3661 aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca
     3721 cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt
     3781 ggccgggctt attatcgcag atgcaagctt ttaaacaccg tttaaggaag aggtcgtgag
     3841 cctgccgttc aactctgagt cacaaggagc aatttcaaaa aacagcttct catgtttgat
     3901 gactattgtg acttgtgacc gaggagttgg catgagaaca ccgtttaaga aaaaggttgt
     3961 gaaatcaacg ttttgttctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
     4021 tgccgtttgt tgagctaata ccgaggatca gatgattgaa caccgtttat tcggaacaag
     4081 ggactgtgac atgacttttg tcacagttcc attatttttt tagtagcttc aaagcttttt
     4141 ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca
     4201 atagttttgc cgctacttgg tcagatttcg ttccagctcc tgacggaagg gtgattccag
     4261 cttcagctga tgctttgtct aactcttcca aaaagctggc acgactgatg atagaagcag
     4321 cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt
     4381 gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca
     4441 aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt
     4501 gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg tatttttctg
     4561 gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga
     4621 tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct
     4681 gatcacgttt cacataagca gcacatactg ttagcggacc gaaatagctg ccatttccga
     4741 cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt
     4801 ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat
     4861 tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt
     4921 ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt
     4981 tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct
     5041 cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga
     5101 gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg
     5161 agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga
     5221 aatgattcgt atggcgcacg aaaaaacacg ctacaaagcg gtgattgcca accaaacata
     5281 tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga
     5341 acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt
     5401 gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatat tggagctgga
     5461 agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt
     5521 gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc
     5581 agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg
     5641 acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa
     5701 aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat
     5761 tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata
     5821 tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt
     5881 atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt
     5941 tgcctttcac ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat
     6001 tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat
     6061 cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac
     6121 tatatttttg attttgcagt tacttgcctt tgttccatta gatgtggtcc aaaatcaatt
     6181 cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta ccaacaagat
     6241 gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga aaatatttcg
     6301 ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg cacagctctt
     6361 ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa cattggaatt
     6421 tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa aagaagaact
     6481 ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc aagaaactga
     6541 ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaaa ttgaaaatat
     6601 ccgtccccat atgaaacgga ttgagatcgg agcagacttg aatggagtgg agcttgcaca
     6661 agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg atctttctga
     6721 cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca cgatccctgt
     6781 tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg atgacgcttc
     6841 tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca
     6901 gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga tcatcaccat
     6961 gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt
     7021 agtccacgat caaagtgctt caggtcagac tttatttatt gaaccaaaac aagtggtgga
     7081 tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc aacgtatttt
     7141 aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca atacttatgt
     7201 gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag taaaagccat
     7261 cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac atccactgat
     7321 cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc aagccatcgt
     7381 cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag gtctgttaca
     7441 actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt
     7501 tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc tatcgacctt
     7561 ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt
     7621 attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc
     7681 aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc attatcctga
     7741 attgaaagtt tatggatata atcgggcaaa tacgattaat gccagcatgg agtttgatgt
     7801 ggatacatta agtccaacgt atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt
     7861 tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga agcagttgat
     7921 gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc gccggaaaat
     7981 ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag aacttcacga
     8041 tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc
     8101 aaagaaaaaa gcaaatgaag tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc
     8161 tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc atcagttgat
     8221 cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga aaaataaagt
     8281 attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac
     8341 ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg
     8401 tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc aaaaagagcc
     8461 gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt
     8521 ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat atttggattc
     8581 tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa caggcgcact
     8641 tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc
     8701 accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa gcttactaaa
     8761 ggtaagcaga tggttcgcta tccaagtata gaaatgctat actagtcata gattttaaaa
     8821 atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga agaaacagat
     8881 aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg tatgcaagca
     8941 ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat tgctaaaatg
     9001 gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat tccaacatta
     9061 atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc aaaagaacaa
     9121 ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag ctgagggtgg
     9181 gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat tcaaattaat
     9241 attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac atgctataat
     9301 aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa acaaattagc
     9361 tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa cgatcattta
     9421 tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg gtagccataa
     9481 tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg ttacagaatc
     9541 gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc ctaaatacaa
     9601 cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg aaaaatatcc
     9661 ccgtttggtg attactcgta aggtactaaa agacaaagca ctgtattttg gaccatatcc
     9721 cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc ctctgagaaa
     9781 atgcaaacct agtcagacga aggagccttg tctttactat catttgggac aatgcctgtg
     9841 tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa
     9901 ctttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc
     9961 tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc gtgccattga
    10021 gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag atgtatttgg
    10081 ttatgcgatt gataaaggat ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct
    10141 aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag atttcttgac
    10201 ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag tcttgattcc
    10261 tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa
    10321 acgaggagaa aagaaaaaat tagttgaatt ggcgagtaag aatgcctcag ttgctttgaa
    10381 tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag ttgaacgttt
    10441 aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata actctaatat
    10501 catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa
    10561 agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg catcaatgag
    10621 agaagtcatc tatcgcagat attcgcgagt gattcgcgaa gggttgcctt tacctgattt
    10681 gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct tagaaaatca
    10741 attaggaatc gatattccta ttgctggcat ggtaaaaaac gataaacata aaacaaatga
    10801 gttgctattc ggtacagatt tggctgtagt accgcttgag cgaaattcac cagagttttt
    10861 cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc atcggcagct
    10921 acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaagggttag gaccaaaacg
    10981 aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag ccacgacaga
    11041 agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca
    11101 tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat tttatgtaca
    11161 acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct ggtgtctaag
    11221 gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg atcttgttaa
    11281 aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg atggaaaaat
    11341 catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt tgatcttgga
    11401 ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg gggagaaaga
    11461 ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag taacgattca
    11521 agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga ttgaaccaaa
    11581 aatcgacttt tggatggaaa aatttcaggt aaaagggaaa aagacggata aagtaaaatc
    11641 tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa
    11701 attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg aattgctaaa
    11761 agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta gccacaatat
    11821 ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa aaatggtttt
    11881 gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat tcttggaatc
    11941 tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga
    12001 agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa tctttactcg
    12061 tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt tggaagagat
    12121 tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc aaagatgtct
    12181 atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct tttatcatgc
    12241 tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg
    12301 gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa acagatgatt
    12361 ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa
    12421 tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg ctttatagcg
    12481 aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact ggcttgcaat
    12541 cgatgcttcg agcgagtcaa ttaggcattt caccagaaga agtagctagc ttgagccaac
    12601 aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg acagtcggag
    12661 aagataactc gacagtccaa tacgtcgtaa gttatgtagc aacgattgtc ttgttcatta
    12721 ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga
    12781 tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga aaattaacag
    12841 gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt ggtataggat
    12901 acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc tcattggata
    12961 acttgattgg accattcttg tggtattctc ttctattcat gatctttggc attttgattt
    13021 tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat acagcaaaag
    13081 caattttgcc agtgacttat ctatctctag gcggatacat gcttggattg atactaggag
    13141 cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc ttatcttcat
    13201 atattatgcc gattcgctta gcaaatgaga cagtcggtat gggtggagca ttgatttcct
    13261 tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac atgtacaaat
    13321 cgaatgtact cgtttatagc gaaggcggat tatggagttc attgaaacaa tcgatttcaa
    13381 ttatgaggaa tgaacgaaaa aaagcataaa acaatcctaa aatacagaag gaagctggac
    13441 atctattgtt cccagcttct ttttgtatag acctatttta tttattttac tatattggat
    13501 atatattcat tgaaaatagt gtataacaac tttataatat tgagtatacc aatttttgga
    13561 ggcgttttaa tggaccgtta tttagattta ctttcagaaa aattcccctc aacggaggat
    13621 gtcatcacgg aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta
    13681 tttttgagtg atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca
    13741 ggaaatctta aagaaaagat caaagacctt ttttctgaac aaatgacgga aacggatatc
    13801 agccaattca ctatttttac ggcttatcca gaatatgcat taacgactga ttggtacaaa
    13861 gaacaagata aatcgcagct gatccatcat ttgatcgatc ttttacggtt tacgacagtc
    13921 aaatataccc ggtcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag
    13981 gaactcttgt atatcgatga ccgtatcaat ggaaaaaaag actatgtaaa aaaaatcatt
    14041 gaacaacttg ttttgatgaa ggaagaagaa aaatttttaa cgaaacttgc acagacgatc
    14101 caaaaatctg tcatcgatca tttgcatatc gttggagata tttttgaccg aggaactcaa
    14161 gcagccaaag tgatggatcg aatcatggat ttttcttctg tagatatcca atggggaaat
    14221 catgatattt tgtggattgg tgcgtattgc ggctctgagg catgtttact gacactttta
    14281 cggattgcag cacgctataa ttatttattt gaattagaga aagaatatgg gttgaattta
    14341 cgtccattgt tttcttttgc tcatcaaact tatcagaaga atccagtatt tgcgcctaaa
    14401 aacagtaaaa atctatccga aagagagcaa gaagaattag aaaaaatcca tcaggcactt
    14461 tctatccttc aattcaaatc agaacatcag ttattggatc gtcgacctga atttaaaatg
    14521 gacgatcgta aattgctgga aaaaatcgac tatgaagcat caacgattga tttagaaggt
    14581 cagctctatg agttgaaagg aacttgtttt caaacgatcc agccagatga tccaaaaaaa
    14641 ttcttggaag aggaacaaaa agtgattgat agtttgatgt attcattcca gcactctttg
    14701 agaatgcaaa aacatatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat
    14761 cagcatgtgc tgtttcatgg ctgtatacct gtagacgaga agggacagct cttagcattt
    14821 gaattagacc aatcttatcg cggaaaagaa ctgctgggct tttttgaaaa acagataact
    14881 gaaagcgcaa aagatctgac tgcaaaagaa gatttagcaa ccgatttgat ttggtatgcg
    14941 tggagtgggc cattttcgcc tttattcggc aagagtaaaa tggcgacttt tgaacgttat
    15001 tttctcacag agcctcatac gcacgtcgag aaaagcaatc cttattatca cttgcgagat
    15061 gaagagtggt tcagtgaaaa aattttagca gaattcgaca caaaagaaga agggtcagca
    15121 atcattaatg ggcacacgcc agtaaaagta aaaaaagggg agtcaccgat caaagcaaat
    15181 ggaaaagtat ttgtcattga cggaggttta tccaaagctt atcaaaaaac gacaggaatt
    15241 gccggttatt cactattatg caattcttat ggtttccaaa tcgtgacaca ttatccgttt
    15301 ttgcctattg aacaacaatt tgcaaaaaaa tccgaccaaa caaatgtgaa aaaagtaatc
    15361 gaacagcaac taccacgcaa actcaatcgt gatacaacaa aaggtatgga actgcagcag
    15421 cagattaccc agctcaaaag gctgttggac tatcgaaagg acgattgaga agaattccag
    15481 tgaacattgt tccatagact ggagactgag acaaaagtcg tgtcgcagtc tctttttccg
    15541 gataaacggt gttcttctgc caactcctcg gtcacaagtc acactattca acaaacatga
    15601 aaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgacctc
    15661 ttggtataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
    15721 gtgaaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacaa
    15781 ccccctcaga aagtctatta aaattcattc acaaagaaat cgaataagcg ctgttcagat
    15841 ggatgtacac ggtgggtaag ttccggatgc cattgaacac cgaggatttt atgcatatca
    15901 tctttgcttt gaaccgcttc aacgagtcca tcgttagaaa aagcgatggc ttttaataaa
    15961 ggtgaaagat ctttgactgc ctgatgatgg taagaattca caaaatatgg atcggggaga
    16021 aggttgctta acaaactgtc tgaaacaatc ttgacttcgt gagtcgtaaa ttgcggggca
    16081 gtgggctgtt gttcatgttt tactgaccac tttggatata atgaaagatc ttgataaagt
    16141 gtaccttcca aaacgacatt caatagttgg agtccccggc agactgcaaa aattggtttt
    16201 ctttgtttca atgcttccaa gatcagtgcc tgttcaaatt ggtctcgttg aatatttgtt
    16261 tccgttagct ttggatgagg atcttccatg taaagttgtg gactgacatc ttgaccacct
    16321 gcaagaataa gtttgtcgat ttgtccaatc agttgaggag ctgaatctgg agacatgact
    16381 ggcagaataa gaggcaatcc ttgagcatct aaaactgcat ccacgaatcc ttgcggtgta
    16441 tagctcactt gatttccttg gaaagtatct gttgctcgaa tcagttgatt tccggcaatg
    16501 ccgatgattg gtttcatttg tattccccct aaagtagact tattgtaaca aggatttaaa
    16561 aaaaattaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
    16621 gtaaagagaa atttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
    16681 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
    16741 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
    16801 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
    16861 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgaa aagcgtgctc
    16921 aaacactgcg gcagcagctg aagatctatg caacagaaaa agagcaggaa ttggaaacga
    16981 tccaaacaaa ctatcgacaa caactaaaac agttggtgga agaaaaagaa caatttgaaa
    17041 tacaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
    17101 ctttgaaaga tcaatatcaa aagcatcaga gtcaagcgat cggtgcgatc attgaaaggg
    17161 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
    17221 aatcaggaga ggattttaca agcagtacaa ggaatccaaa acgttgaaat ccgggatctg
    17281 tatcaaaata gaacaaacaa ccagtgggtg gagaagtatt ttccagatac cacgattatt
    17341 gataaagaag caaaattaag cgctttaggt aatcggttga aagacatcca agaagctatc
    17401 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa
    17461 ctttccttac aagatttgga agccaattat tccgaagaaa atttcataaa taaattgaaa
    17521 gaagtccttg atttaaaaga aaagtgggaa caactaactg aagaacgtga tcgtttagaa
    17581 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
    17641 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
    17701 gaagaactga gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
    17761 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ttatagaaga aattgctggg
    17821 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaactgcaa
    17881 gagaccaagc agcaattatc cattgttcac gagcagcaaa agaagataac gacagcgtta
    17941 ggtcagtgca gtggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
    18001 gcagaacgag aaaaaataaa agaacggttt atccatgcga cttatctgat cgttttgcaa
    18061 ggttgggtag atgcagaaga aaaacagaat ttgctgcatg tgttaaacga aaccgtatca
    18121 gaagaagata tatacgtcag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
    18181 cctactaaat tgaaaaacca tccgcttgtc gaaccttttg aattgctgac tgaaatgtac
    18241 agtctgccaa aatatgaaga agtagatcca acaccttgga tgacaccatt ttatcttgtc
    18301 ttcttcggga tgatggtcgc agatgttggt tatggtttgc tgatgctcat tgggacaata
    18361 ctagctcaaa aactacttgt tctaccacgc gggatgaaac gtttcgtaaa attctttgag
    18421 attctctcga taccatcgat catctgggga ttgatctaca gttcattttt cggacagtca
    18481 ttaccaaaag aactgtttgg catccgcttg cctttcccaa tcctatctac gacggatgac
    18541 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatttt ggttggatta
    18601 tttgtggcag caaaggaaaa tatcaggaga aaagattact tgagtgctgt cagtgatgga
    18661 tttgcctggc aagggatcct aattgggatc gttattgcgt tggttggtgc catgttgata
    18721 aaaaatacag catttgttta ccttggcgct gggctatcga tcattgctgc cttatgtatc
    18781 ttgatcgttc cgattatcca aacgccgtct aaagtcaaag gtgctgccaa aggtgcgtat
    18841 aatctttacg gactgaccgg ctatatcggt gatttagtca gttatacacg tttgatggca
    18901 ttgggtattt caggcggaag tattggagcg gcattcaata tgctagttgc tttcatgccg
    18961 ccagctgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
    19021 tttttgacac tgctaagtgc ctatgtccat ggcgcacgat tgcagtatgt cgaatttttt
    19081 gggaagtttt atacaggagg cggacgtgcc tttgatccgc tgaaaacggc ggaaaaatac
    19141 gtcaacatca atcacaagaa aaaaaacaat aaataattgg aggattttta tataatgatg
    19201 gattacttaa ttagtcaaaa tggtggaatg gtatttgcgg tattagcgat ggcaacagcg
    19261 acgatttttt cagggatcgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
    19321 gcattgacaa caagtcaacc cgaaaaattc gggcaggctc tgattctgca attgcttcca
    19381 ggcacgcaag gtttgtatgg attcgttatt gcctttttga tttttatcaa cttaggaaat
    19441 gacatgtcag ttgtacaagg actgaacttt ctaggtgctt ctctgccgat tgcttttaca
    19501 ggcttgtttt caggtattgc ccaaggaaaa gtcgcagcgg caggtattca aattttagcg
    19561 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatacgcg
    19621 attttaggtt tcgttatttc cttcctatta gtgttgaatg cctaagcata aaggaggaaa
    19681 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa cgaagcagca gagcaggaaa
    19741 gagcggcgtt agaacaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
    19801 cgcaagttga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta ctcgaaaaaa
    19861 aataccgtca gctaaggaat cgccagcaag tagaagtacg acaagaaaat ttgaatgcga
    19921 agcaggaatt tttaagacga ttgtttgcag atgcggtaac tgaaatggaa aattgggatg
    19981 aatctgaaca aatccagttt atcaaaaatg cattgtattc attacctttg acaggaaaag
    20041 tagcattcat tgctggagaa aaatcagcag cctatctttc tcagacgttg ttggatgaat
    20101 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
    20161 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
    20221 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
    20281 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
    20341 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
    20401 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
    20461 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
    20521 aatcgtttgg atctatacga tgcgttatac tttccataat ctgaaggttt tgacaaaagc
    20581 tgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
    20641 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa cttcctgaaa gcattatgga
    20701 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
    20761 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
    20821 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
    20881 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
    20941 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
    21001 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatattga
    21061 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
    21121 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    21181 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaatcag aaacaatcag
    21241 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    21301 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    21361 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    21421 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    21481 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    21541 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    21601 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    21661 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    21721 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    21781 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    21841 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    21901 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    21961 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    22021 aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg
    22081 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    22141 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    22201 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    22261 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    22321 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    22381 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    22441 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    22501 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    22561 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    22621 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    22681 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    22741 gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac
    22801 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    22861 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    22921 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    22981 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    23041 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    23101 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    23161 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    23221 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    23281 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    23341 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    23401 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    23461 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    23521 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    23581 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    23641 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    23701 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    23761 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    23821 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    23881 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct
    23941 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    24001 aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct
    24061 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    24121 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    24181 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    24241 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    24301 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    24361 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    24421 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    24481 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa
    24541 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    24601 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    24661 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    24721 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    24781 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    24841 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    24901 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    24961 gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    25021 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    25081 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    25141 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    25201 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    25261 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    25321 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    25381 gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    25441 agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    25501 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    25561 atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag
    25621 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    25681 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    25741 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    25801 tcatgcttta catcctaaaa ttggcctttg tgatccaatt gattggtacg gttgctttat
    25861 cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    25921 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    25981 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    26041 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa
    26101 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    26161 cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    26221 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    26281 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga
    26341 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt
    26401 tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    26461 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    26521 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    26581 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    26641 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    26701 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    26761 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    26821 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    26881 ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt
    26941 gattccttta tttatcaata ggttataatt catatttttt tattttttct tttacgatac
    27001 ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat
    27061 ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa
    27121 agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag
    27181 ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga ttggatcttt
    27241 ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag
    27301 tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta
    27361 tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa
    27421 attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa
    27481 tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata
    27541 gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc
    27601 ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt
    27661 cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta
    27721 aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc
    27781 tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa
    27841 caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag
    27901 tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg
    27961 ggttattact agttgattga ataaacggga caacgaattt attacattct tcaaaagaac
    28021 taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact
    28081 caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc aaacctttct
    28141 caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta
    28201 gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt
    28261 ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc
    28321 aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta
    28381 taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg
    28441 aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag
    28501 cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc
    28561 ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg
    28621 cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc
    28681 atcagtatca tttaccttct catttaggag agattttctt gtttgatgct caaaacggac
    28741 tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg
    28801 cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg
    28861 gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt
    28921 ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt
    28981 atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa
    29041 tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag
    29101 acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt
    29161 tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg
    29221 acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag
    29281 gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg
    29341 acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag
    29401 agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt
    29461 gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat
    29521 gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca
    29581 agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac
    29641 attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt
    29701 ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt
    29761 tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc
    29821 taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac
    29881 ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt
    29941 catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt
    30001 aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc
    30061 agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct
    30121 agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa
    30181 aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc
    30241 aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag
    30301 tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc
    30361 atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt
    30421 aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagacca gcagctttat
    30481 gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc
    30541 ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag
    30601 gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt
    30661 caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac
    30721 atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa
    30781 agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat
    30841 agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa
    30901 agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc
    30961 agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag
    31021 gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg
    31081 cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa
    31141 cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac
    31201 caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga
    31261 aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga
    31321 aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt
    31381 gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca
    31441 agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg
    31501 ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga
    31561 ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca
    31621 cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct
    31681 aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct
    31741 tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg
    31801 ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg
    31861 acacggcgtg ccattacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg
    31921 atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca
    31981 ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca
    32041 gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct
    32101 tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg
    32161 atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata
    32221 tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga
    32281 tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca
    32341 gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg
    32401 atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta
    32461 gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt
    32521 tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga
    32581 ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta
    32641 aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg
    32701 tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac
    32761 aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga
    32821 tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc
    32881 atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg
    32941 gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc
    33001 attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt
    33061 ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga
    33121 tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg
    33181 aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca
    33241 cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa
    33301 aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga
    33361 gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg
    33421 cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca
    33481 tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta
    33541 taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg
    33601 gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc
    33661 acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa
    33721 aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata
    33781 gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc
    33841 acgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat
    33901 ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg
    33961 acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt
    34021 caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg
    34081 gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt
    34141 tctgatcact ggagcgtaac ctgcatcttc cagtgtttgg atcccgtcac ttagaaaagg
    34201 agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa
    34261 gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa
    34321 gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc
    34381 tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa
    34441 aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat
    34501 gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca
    34561 atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg
    34621 aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag
    34681 gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg
    34741 atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca
    34801 tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt
    34861 agtttgaaag accgtcccac agcagggaag gttcgaatcg attatgatct gttcatggga
    34921 ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa
    34981 agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc
    35041 tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta
    35101 gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag
    35161 atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat
    35221 actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt
    35281 tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat
    35341 gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa
    35401 gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac
    35461 gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac
    35521 ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc
    35581 gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa
    35641 ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga
    35701 tcagcctatc agaataagga ggtgtgacac gattttt
//
LOCUS       DACCIQ010000023        32533 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCIQ010000023 DACCIQ010000000
VERSION     DACCIQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.023"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            131..505
                     /locus_tag="IVM62_001122"
     CDS             131..505
                     /locus_tag="IVM62_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6845093.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVM62_001123"
     CDS             complement(619..1248)
                     /locus_tag="IVM62_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6845094.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVM62_001124"
     tRNA            complement(1382..1454)
                     /locus_tag="IVM62_001124"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVM62_001125"
     tRNA            1668..1741
                     /locus_tag="IVM62_001125"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVM62_001126"
     CDS             1696..1845
                     /locus_tag="IVM62_001126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845095.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            complement(1879..2052)
                     /locus_tag="IVM62_001127"
     CDS             complement(1879..2052)
                     /locus_tag="IVM62_001127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845096.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="IVM62_001128"
     CDS             2338..3666
                     /locus_tag="IVM62_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6845097.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVM62_001129"
     CDS             3868..4701
                     /locus_tag="IVM62_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP6845098.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVM62_001130"
     CDS             4717..5454
                     /locus_tag="IVM62_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP6845099.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVM62_001131"
     CDS             complement(5609..6577)
                     /locus_tag="IVM62_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP6845100.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVM62_001132"
     CDS             6809..7642
                     /locus_tag="IVM62_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP6845101.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IVM62_001133"
     CDS             7676..8515
                     /locus_tag="IVM62_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6845102.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IVM62_001134"
     CDS             8541..9101
                     /locus_tag="IVM62_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6845103.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IVM62_001135"
     CDS             9300..11021
                     /locus_tag="IVM62_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP6845104.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IVM62_001136"
     CDS             11186..12328
                     /locus_tag="IVM62_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6845105.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IVM62_001137"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IVM62_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP6845106.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IVM62_001138"
     CDS             14128..14775
                     /locus_tag="IVM62_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6845107.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IVM62_001139"
     CDS             15168..17813
                     /locus_tag="IVM62_001139"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP6845108.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IVM62_001140"
     CDS             18123..19436
                     /locus_tag="IVM62_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP6845109.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IVM62_001141"
     CDS             19426..20079
                     /locus_tag="IVM62_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6845110.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IVM62_001142"
     CDS             20134..20826
                     /locus_tag="IVM62_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP6845111.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IVM62_001143"
     CDS             20976..21176
                     /locus_tag="IVM62_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6845112.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IVM62_001144"
     CDS             complement(21430..23109)
                     /locus_tag="IVM62_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6845113.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IVM62_001145"
     CDS             complement(23111..23323)
                     /locus_tag="IVM62_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP6845114.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IVM62_001146"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IVM62_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP6845115.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IVM62_001147"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IVM62_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP6845116.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IVM62_001148"
     CDS             27298..27495
                     /locus_tag="IVM62_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845117.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="IVM62_001149"
     CDS             27648..28133
                     /locus_tag="IVM62_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP6845118.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IVM62_001150"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IVM62_001150"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP6845119.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="IVM62_001151"
     CDS             29010..29537
                     /locus_tag="IVM62_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP6845120.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="IVM62_001152"
     CDS             29628..30365
                     /locus_tag="IVM62_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6845121.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="IVM62_001153"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="IVM62_001153"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP6845122.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="IVM62_001154"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="IVM62_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP6845123.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5523 c         6874 g         9364 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacatcc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc atttttttta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cctatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DACCIQ010000024        31081 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCIQ010000024 DACCIQ010000000
VERSION     DACCIQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.024"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="IVM62_001155"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="IVM62_001155"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP6845124.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="IVM62_001156"
     CDS             complement(1061..1981)
                     /locus_tag="IVM62_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6845125.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="IVM62_001157"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="IVM62_001157"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP6845126.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3833..4366
                     /locus_tag="IVM62_001158"
     CDS             3833..4366
                     /locus_tag="IVM62_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP6845127.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4856..5380)
                     /locus_tag="IVM62_001159"
     CDS             complement(4856..5380)
                     /locus_tag="IVM62_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845128.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IVM62_001160"
     CDS             complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IVM62_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP6845129.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IVM62_001161"
     CDS             complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IVM62_001161"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP6845130.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7102..7782)
                     /locus_tag="IVM62_001162"
     CDS             complement(7102..7782)
                     /locus_tag="IVM62_001162"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6845131.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7908..8777
                     /locus_tag="IVM62_001163"
     CDS             7908..8777
                     /locus_tag="IVM62_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6845132.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8803..9318
                     /locus_tag="IVM62_001164"
     CDS             8803..9318
                     /locus_tag="IVM62_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845133.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9365..9928)
                     /locus_tag="IVM62_001165"
     CDS             complement(9365..9928)
                     /locus_tag="IVM62_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845134.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9983..10162)
                     /locus_tag="IVM62_001166"
     CDS             complement(9983..10162)
                     /locus_tag="IVM62_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845135.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10239..10748)
                     /locus_tag="IVM62_001167"
     CDS             complement(10239..10748)
                     /locus_tag="IVM62_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845136.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10844..11224)
                     /locus_tag="IVM62_001168"
     CDS             complement(10844..11224)
                     /locus_tag="IVM62_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6845137.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11554..12303
                     /locus_tag="IVM62_001169"
     CDS             11554..12303
                     /locus_tag="IVM62_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP6845138.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12487..13764)
                     /locus_tag="IVM62_001170"
     CDS             complement(12487..13764)
                     /locus_tag="IVM62_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845139.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13843..14487)
                     /gene="pcp"
                     /locus_tag="IVM62_001171"
     CDS             complement(13843..14487)
                     /gene="pcp"
                     /locus_tag="IVM62_001171"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6845140.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14686..15630)
                     /locus_tag="IVM62_001172"
     CDS             complement(14686..15630)
                     /locus_tag="IVM62_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6845141.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15632..16318)
                     /locus_tag="IVM62_001173"
     CDS             complement(15632..16318)
                     /locus_tag="IVM62_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6845142.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16792..17112
                     /locus_tag="IVM62_001174"
     CDS             16792..17112
                     /locus_tag="IVM62_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6845143.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17266..18171
                     /locus_tag="IVM62_001175"
     CDS             17266..18171
                     /locus_tag="IVM62_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6845144.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18168..18941
                     /locus_tag="IVM62_001176"
     CDS             18168..18941
                     /locus_tag="IVM62_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6845145.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(19122..20546)
                     /locus_tag="IVM62_001177"
     CDS             complement(19122..20546)
                     /locus_tag="IVM62_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845146.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20757..21203
                     /gene="nrdI"
                     /locus_tag="IVM62_001178"
     CDS             20757..21203
                     /gene="nrdI"
                     /locus_tag="IVM62_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6845147.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21268..23250)
                     /locus_tag="IVM62_001179"
     CDS             complement(21268..23250)
                     /locus_tag="IVM62_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6845148.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23264..24160)
                     /gene="murQ"
                     /locus_tag="IVM62_001180"
     CDS             complement(23264..24160)
                     /gene="murQ"
                     /locus_tag="IVM62_001180"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6845149.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24163..25224)
                     /locus_tag="IVM62_001181"
     CDS             complement(24163..25224)
                     /locus_tag="IVM62_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6845150.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25435..25962)
                     /locus_tag="IVM62_001182"
     CDS             complement(25435..25962)
                     /locus_tag="IVM62_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6845151.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(26029..27531)
                     /locus_tag="IVM62_001183"
     CDS             complement(26029..27531)
                     /locus_tag="IVM62_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6845152.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27789..28910)
                     /locus_tag="IVM62_001184"
     CDS             complement(27789..28910)
                     /locus_tag="IVM62_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6845153.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            29036..29977
                     /locus_tag="IVM62_001185"
     CDS             29036..29977
                     /locus_tag="IVM62_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6845154.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(30127..31023)
                     /locus_tag="IVM62_001186"
     CDS             complement(30127..31023)
                     /locus_tag="IVM62_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6845155.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         8992 a         6499 c         5510 g        10080 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ctgtttatcg agagattgtg aaaaagctgt cctgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3181 ccgcagatac aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3241 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg
     3361 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttggac
     3421 ttattaccgc agatgcaagc ttttgaacac cgtttattga gaagttgtga aatcagcgtc
     3481 ctgacctgag tattagagaa acaaatggca aaatagttcc tcatattttg ctattttttg
     3541 agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg
     3601 cctactttcg agtcgtaaga aatttttttg aaaatggttc ctcacaactt tgcataaatt
     3661 tgacttaata ccgagaagtc atgatcccca tctaactcca aatcacaagg aatgagttca
     3721 aaaactagct tttctcaaca aatattacat aaaaaattct atgttttttg ttaaatttat
     3781 ttgctttttt caaaagtatg ttacagtaat ttcccaagga ggttttacag aaatgaattt
     3841 tgttgccatg gattttgaaa ctgccaatca tcagccttat agtgcttgtt ctcttgcgct
     3901 agtcatggta aaaaatagcc agatcgttga tgaattctat acattgatac agcctgagac
     3961 tccttttttc tggcgaaatg tccaaattca cggaatccat caagaagacg tacgcaatgc
     4021 gccgaaattc ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt
     4081 cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag attattatca
     4141 tcttgaacag cctaattatc tttctttatg tacagtgaaa agtagccgtc gcctgtttcc
     4201 tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc aattagatca
     4261 tcaccatgat gcattggaag atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa
     4321 aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga atacttatga
     4381 aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag caatttcaaa
     4441 aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg gcaggagaac
     4501 accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
     4561 caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat gcaagctttt
     4621 ggacaccgtt taccaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
     4681 acggcaaaat agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat
     4741 tgaacaccgt ttattcaaaa aaaatggact gcgacacgac ttttgtcaca gtcccttcgt
     4801 gtgtttttct gccttctcaa aaggatgaca gtttctcact cttttcctat ttcttctatt
     4861 caatcaaata actcatttta tatacatcta aaaattcacc tagatctgtt cttgcccctc
     4921 taggcatgac cgcttcgatt tggaagccca tttttcggta aaggtgtacg gctctttcat
     4981 tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc catttccttc gcccaactga
     5041 tgatttcttc tagcataaga gtgcctagtc ccattcccca atattcctgc agaatactga
     5101 taccgacttc tcccacgtga gacaaacgaa attgactgtc tgccttgaca gatgctgttc
     5161 caataatcgt cccttcatag attgcaagca gcaataaatt attattgctt tcgtatatat
     5221 aatccaactc ttcttctaag gtttctggtg gtaacaggag ttctgcttca tccaaaacta
     5281 aaaattctgt ctccttatta acttgtccca tcatcaaaag aatgtctttt gcatctcctg
     5341 gcactgcttc ccgcaagatg atcccaactt cttcactcat gattttcagc ccatttttct
     5401 agaattcgct gtttgaaggt ttcactttgc tccccgtatg agattagttt cacataccgc
     5461 ttttctaaat ctgtatcact tttttctaaa atcagttcca aataatcctc cggcagtgct
     5521 tcttctaata gatttcccca ttcaacaacg gaaagcccat caccttcaaa atattcatct
     5581 aaccctaaat ctgctccgct agctgctacc cgataaatat ccatatgata caacggaatc
     5641 cgaccttgac tatattctct aataatcgtg tatgtcggac ttttgatcat ttgcgttatc
     5701 cctaatcctt gggcaattcc tttcgtcaac gttgttttac ccgcacctag atcaccagtc
     5761 aatatcagat tgtcacctgg cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc
     5821 attgttaatc ctgatagttc gatcattttt catcttctcc cgctcttctt tctttttgcc
     5881 agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg
     5941 ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa
     6001 tcgataattt atatacatct tcttcatttg ctccacgaga caaatcagaa accggtttgt
     6061 tcaagccttg tagaattggt ccgattgctt caaagttacc gaaacgctga gcaattttat
     6121 agccgatatt tcctgactgc aattctggaa atacaaagac tgttgcttga ccagctactt
     6181 ctgaatctgg cgctttcagt tgtgcaactg aaggaacata agaagcatca aattgcaatt
     6241 cgccgtcgat catgtattgt ggcgctagtt ttttagcgat tttagttgct tcaactactt
     6301 tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa gctcatcatt gctacttttg
     6361 gttcgatatc gaacaattct gctgtttttg cagaatctac ggcgatttca gccaattctt
     6421 gcgcagttgg attcacgttg atcgcacaat ccgaaaagac atatttttct tgatctcttc
     6481 cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct
     6541 gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc tccagagacc attccgtctg
     6601 caatccccat atacgtaagc atcgtaccaa agtaattcac atcttttaaa atcttttctg
     6661 cttgttcttt ggatgccttt ccatttctac gttcaacaaa agtatccacc atttcatccc
     6721 acttatcgta attatcaggg ctgatgatcg taaagctaga tgttttgatt cctctggcat
     6781 gcgctgcatt tcttatttct tcttcgttcc ctactaaaat gggttccaca agttcttctg
     6841 ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt agcttccggg aaaacgattt
     6901 tgatcccacg gcgaacaatt ttaaatctta aactatcaaa caattccaca agatgacctc
     6961 cagacgtttt tatatgtatt atcatacact attccagaaa aaaagaacag tttattttat
     7021 ctgtatcagt caaaaatgct ggaaaaatta agtttttcac atacaaagcg aactgcacta
     7081 taacaggaaa gcgctgtttt attacactgt ttcaggcagt tgccaatcaa tcggttcttc
     7141 tcctaacgca aggagcgcat cattcgtttt cgagaagggt ttggaaccaa aaaagccacg
     7201 acttgcagat aacggactgg gatgtggaga tgttatgatg atatggcgac tcttatcgat
     7261 cattttgatt ttttcttgtg ccggttttcc ccacaagata aacacgattg gtttttcccg
     7321 ttcatttaat ttttcaatga tcgtgtctgt taatctttcc caacctttcc cgcgatgaga
     7381 atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt aaaaggactc cctgtttagc
     7441 ccatgacact agatttccat gttgaacagg tgcgattcca agatcatcat aaagctcttt
     7501 atagatattt cttaacgatg gcgggatctt tacgccaggt tgcacagaaa aagataagcc
     7561 atgagcttga ttttctccat gataaggatc ttgtcctaaa atcaccactt taacttcttc
     7621 ataaggagtc aattccaatg cttcatagat atgatacata tctggatgga tcttttggga
     7681 cgcatattct ttttttaaaa attctcttag atgcagataa taatcttttg aaaattctga
     7741 ggacaaaact tcttgccaac tgttatgaat gatttctttc aaaaactcca cctcctaata
     7801 aaaagaataa ctcacatttt cttcaatcac aagggacaat cttcaccctc agcacttgat
     7861 taatatggta aactaggaac aagaagcaga aaggaacgat aatcattatg atcaaattaa
     7921 ttgcatcaga tatggacggc acattattag atgcccatat gagtgtttct cctgaaaatg
     7981 ccgaggcgat acgttttgca aatgatgccg gtgttgagtt catggtcgct actggtcgaa
     8041 actatcaaga agctcgcgca gctttagatg aggtaggaat cgattgtgcg atgatcactt
     8101 tgaacggggc acaggtattt gataaagaag gaaattcatt gtttaccgtg tcgattccaa
     8161 ataatcaagc aatctctgtt ttagatattc tagatgctaa cggtatttac tatgaagtag
     8221 caaccaacga tggtctttat tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca
     8281 tggtggctac ccatttacct caccttacgt ataaaatggc gattgccatg gcctcagcta
     8341 agctggagtt attgcacatc acatacgtgg acagtattcg tgctattttg gaagaaaaga
     8401 atctggaagt tttaaaaatt atttgttttc atacagaagg gccagctgta ttaggtccag
     8461 ttggaaagca gatttctctt ttaggcgatc tagccgttac ttcctctggt caaaacaata
     8521 tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc
     8581 gtgggatttc tttagaagaa gtcatgacga ttggcgataa cttcaatgat gtcagcatgc
     8641 tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg
     8701 ccaaatatac aacggacaca aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga
     8761 ttgcagaaga cttgtagttt tatctagaaa agagggatat agatgtcaga atttttcatt
     8821 caggaaaagc agcttggacg tgcaactcgg acaattgtca aaaacgaaaa aggacattcc
     8881 ctgtttttga tggttggtcg ctggggaaca aagggcgatg cattgtctct ttatgccatg
     8941 aatggcgatc tcgttgctca catcaagcag ataagcctga cattcggtac gcgttttgag
     9001 ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa tattcaattg gcccggtgac
     9061 ttttattata ttcgtagact tcactggaca gtccaaggtg atatctataa tcatcaatat
     9121 caaatccatc atttcaatca gcaaatcatg aaaatggata aagcaacact tttttcagga
     9181 gattactatg tattggatgt ctcaaatgat gaagatgctc cagtttgtat ttgtattgct
     9241 gcggttttag attactggct ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac
     9301 cgacttaact taagttaaaa agatgtacct gaaatgcgga aatacgcatt tcaggtacat
     9361 ctttttagat attcttttgc atatgatcat aaagatgacc actgatcatc cgctgaccga
     9421 caactttgaa gtcatggcgt tcgtataatt ttttagcctt tggatttttt tcatccacac
     9481 ttagaccgat gacggagcga ttagctttct tcgccaattt aggtaatgct tctaataatc
     9541 gcgaaccgat gccttgtcct ctaaaatctt ctgatacact gatagagtcc aaataccatt
     9601 cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga cgcatctagc tgatatttct
     9661 ctaagatagg taccagtggc tgatcgatga ttgattcttc ttcatctgta taaccgtaag
     9721 caactcctgc aactttaccg tctatttctt ctacgatacc tcgtgaataa ctgtaacgat
     9781 aatttggatc agtgattgct tcttctaaaa tctctaaagt cttttgttcc cctgtctgct
     9841 tcaaaaatgg cagttccata tcttttagaa tcactaagat caatggggcg atttctggag
     9901 catcttcttt tctcgcaaaa cgaatcatag tttcctctcc ttttctccat tctaacatgc
     9961 agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc aatcttttca gtaatgaaat
    10021 aaccatgagt aacgattctt gccgcttcag ttcggcaata agggcagacg actggcattt
    10081 ctttatcatt atgttcgact acattattga cttcttcgta tgcaaatttt tttccgcaat
    10141 tagggcattt cattttttcc atgttatctc aacttccttt catctttact ctcttagctt
    10201 actcttatat tgtaaaaaag agaagcaata tccttcttct atgcagatat ccatacctga
    10261 cggtcctgtt ctccatctat taatcggact ttataataag gacgttcatc tttgactaga
    10321 agtgtccact ctttcacttt tgcttttttc ccgtagtagc tgactgcttc cttcatcgct
    10381 gcttgcggtt gtttgaactc ttccgttttt tctatatcaa aaagatgttc tgcggccttt
    10441 tccgtgtcat ttcgtacggt tgttttccca gtggatggat tgactgctat gacttgtcct
    10501 tcacaaaaaa actggtattc atatgttgca ttcgtaggat tctccaaatc tttttcaact
    10561 gcaaaatgaa tagcagatat gttatgttct tttgtttcgt tttttacgat tgctgctgct
    10621 tttttcaatg agatccgata ttcctggtca ctaatatcat ccatgtgaaa agcggcattg
    10681 agcagtcccg tcataccggt aatccctatt acgatgaggg cagcaacacc aaacatcatt
    10741 tttttcattc ttcattctcc tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg
    10801 aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta tctttattcg tgcagttcta
    10861 atggcaatcc gtctggatca aagaaaaacg tcattttttt tcctgtaaag gtatcttctc
    10921 taatcggttc tgattcgatc cctaaggact gcaattctgc aattgtttct tcaatatcgt
    10981 ctactttgaa agcaagatga cgcaagccac aagcttctgg atagtttggt cgttgtggcg
    11041 cattagcaat tccaaaaatc tccaattcag cacttcctag tttcagatct aatttatagt
    11101 catctcgttc tttgcggtaa ttttctctga ttacttcaaa tcctaattta ttaacataaa
    11161 aatcacgtga tttctgataa tctgaaacaa tgatggctat atgatggatc gttgtaaggt
    11221 tcatatgagc actcctattc ttcgtttaag ctcggatcta tattaatctc cagttccatt
    11281 gtaaatgtgt cagattgaaa aaaatagctg tttttccttt ttataagaaa aaaggcaaaa
    11341 aaaacagaaa gtaggtcctc caagacactc ttcctgttcc caatataata aatgaaagta
    11401 agaaattata ttttcaagaa aacaaacaga aaattatcat aacacaaatt atttataatt
    11461 gaaagactag ttttttttca agaaaagcat acaatagatt gtgttagtaa atacagaaca
    11521 cactacattt agtgtcttaa ggagtcctca tacatgaagt catattttca gtttttagct
    11581 catcagctaa aaggctggcc gcaacaaaat tattatcttt tctttttcag tcttggctgc
    11641 caggtcatga ctcttgtcaa tagtcccatc acttggctgt ccgttattac atttatcggt
    11701 accacgcttg gtgtattgtg tattttatcc atcaatgcgg caaaatcagt caacggtatt
    11761 ttaggtattt tgtctgcgat ttgttttatt atcgttgggt tttcagcaaa gaactattta
    11821 agtatcggtg aacagattgc ttatatgatc acattggata tccccgtatt gttaagtaaa
    11881 gaatggaatc ataatatggc ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc
    11941 gcaatcatca gtactttcct tgtctatctt gtttcaggat tcctgattgg tcgttataca
    12001 gatgatcctc ggccgtgggt agatgccatt gctttttcta ttagcttgac tgctggtatc
    12061 atcagtttct tgcgttacaa caaccaatat tactggtggt tagcttcagg gcttgctcaa
    12121 atggtccttt ggtatatttc atttacgcat ggatctgcaa gtttggcgat gttcgtgaac
    12181 agctctgttt acttgctgaa tgatgtcctt gcatttaccg tttcaccttg gtataaccaa
    12241 aaagaacgtg aacgtatgca aaaaatggaa gaagaatact acaacgcaca acaacacgca
    12301 taaaaaaact gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    12361 ataaacggcg gaatcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga
    12421 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
    12481 tattttttat agtaattcct tcagatactt tcttgcctca tcattgaaac gcagtgattc
    12541 ttctaacttc tgataatatt ccaacgcttg tttctgagct tcagtaaaac gataatgttt
    12601 tcctttccat ttgtcgattg tctcttgcac atttccagat tggatatctt tcactactag
    12661 ttgccatttt tcttttgaga ctgtacggtc tttcttatca gcagattgta tagcttctcg
    12721 ttctttacgc aatactttta aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa
    12781 cccttctgta aaaccatagc gacaatattg cttccaaata aatagataga tttttggttg
    12841 gatgaatcgt tggtttttcg caaaatattc catctgagca gtgacttctg gtgttcgttg
    12901 acgttttgtc tttctcaaca cggaaataaa tgtttcaaaa taagcattag gatgtactgg
    12961 cacttcttca tatacgatgg attgccaatc tttacgtaaa aatgcgcgat agttgatcaa
    13021 gaattcttct acatccttta ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat
    13081 ctgtcgcttt tttgactgcc cattgatgtg ttcacttatt ttcttgattt cattttccaa
    13141 tgcttgataa tccgctatgt aaatcggcat ttctacttgg cgaaattctg cgagccgctg
    13201 agctaatttt atatccggta aatatggctg tttcatccga cgattttggt acaagacaaa
    13261 acagtatttt tgccatagtc cttcaggaaa atcgatattc ttctgcttaa gccataataa
    13321 ttcgtctaag gccagatcaa cttttgtcat tccctgatgg atcgaagctg ccactgtcgg
    13381 agagacatgc aggtgatcgg caaagtggtt gatgcctagt tttaggatct ttccggtttt
    13441 tggcgattta acgatgtatt gcacttttaa ttctctacca cactcacaat acaatttttg
    13501 ctggcttcgt tttgttcgaa aaaacggatt gatcttgaag tcagaaaaca cccaatctgt
    13561 tgcagctaaa taatgttctt ccataaaacg agagctgatt agatatcggc ggtgcttatc
    13621 gactagttcc cgctgttttt tcgttaattt tgcatagatc tcccaacgtt gactgcttgt
    13681 gtattgttta gaaattttga tgggatagct tttgctgatt tcttttggaa accattcttc
    13741 aaagacggat tgccattctt ccacggctgt ttctttcctt tctaattaca aaaagctggc
    13801 tactaacata acgttagcaa ccagccagtg tttcttgcta aattagtgag ttgttccggc
    13861 atttccggaa atatcttctt tattttctac tgctgcttca actgcataag tcaaagaaga
    13921 agcaatgtct tctaaagaca tagaaggcgt acccactgga cgatcgatca cttgatcggg
    13981 tgagaatggc acgtggatga acccacctcg aattgccggg aattgtgttt cgatcaaatg
    14041 aagcaaacga tacatgatat cattgcagac aaatgttcct gctgtataag agatgtacgc
    14101 tggtagacca tgatcttgta cattttttgt cattgctttg attggcaatg aggtaaagta
    14161 agcggctgga ccgttttctt ctaaagccgt atcgaatggc tgatttcctt cattatcagg
    14221 gattcttgct tctgctagat tgattgcgac acgttcgaac gagactgctg accgcccacc
    14281 tgcttgtccg acacagatca cgacatctgg ttggtgctgt tggatggctt ctttgactac
    14341 ttgactgctt ttttcgaata cggtcggtac ttccactttg atgatttcag caccgctgat
    14401 ttcatctggc atttttttta ctgcttcata ggctggattc actttgtccc caccaaatgg
    14461 atcaaaacct gttactagta ctttcatctg ttgttcctcc gtccgattga tttaacatat
    14521 ggttatcttt tatcatacca ttttccttga acagcgtaaa taaaggaaac aatagtttaa
    14581 ataacataac agatcaaact taatatattc ttataaaaaa atagtgttcc actacaatgt
    14641 tagaagaggt tgcgacaatt attctgtcac aacccctctc ttctattatt taaatagaat
    14701 catataagca atttggaaaa ctagcataaa taacgcaata aacaactggt ttttgatcac
    14761 tccgtatttg tctttcatct ccagcatcgc tacaggcacg atattgaagt tagcagccat
    14821 cggtgtcagc agggtcccac aaaaaccgca agtcaatgca accattccta ttaacgctgg
    14881 attcgctcca tgactaaata cgaatggtgc accgatccca accgtcatca cggtgatggc
    14941 cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat
    15001 gccgatgttc acatttcctt ctggaacgat cgtacctact gcattggaaa tcactgtccc
    15061 aactccagcg cttgtaaata ccgcacctag agaagctaaa agcattggca acatgcttaa
    15121 cggccctact gtgcccagca tatctgctgc atcatctaaa aatgtacttg gtcgattatc
    15181 tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa
    15241 agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg
    15301 aataaaaatt ttcattccta gtttgtctga cattttttcc atatattctt ttgatggaag
    15361 ccggctttct cctttgctga ctttcttagc aattgctggt atggtcatcg caaagatcaa
    15421 gccaccgttc acgattgccg gaagccatct tcctcctgca ataactaccc ctaagaccgt
    15481 ccagaaaaag gctgacccat aacgatgagg attggtttta tctgttaaat ttcttactcc
    15541 agtgtaaatc aatacgagtc ccatcacgat ataaaggatc tccagtaatt tatcgcctaa
    15601 taaaatatca ggatttgtaa aaaagtccat cttattgccc ctttcctgga taatatttct
    15661 tcgctaattt cttttcttta agatagaagt agacacttgc cacaactaaa gccgtgatag
    15721 ccactggaac ttccactaaa gcaagctgtc ccaatgttac gtcatatcct agatctttca
    15781 gtgttgattg gactagcaac gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc
    15841 agcagacatt ttccatagct gaagccatac cttttagttc ttcttcatgc ttaggatgga
    15901 cttctaaccc ttttgattcg atggtcccca atgccatcgg taaaatgatt ggacgaacaa
    15961 atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa gatcattcga aagatcccat
    16021 aaatatctac aatcatacca gcagatactt tcttgaatcg cttgatcaag gaagccgctg
    16081 attctttcag accatttctt tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg
    16141 ccatcccgcg attgtttaca aaacttgtac ctaaaagttc taacattcct tgaatgccaa
    16201 gaccgccagt caaagctgta acaactaaag caaccattac gatcaatatc gagtctaatt
    16261 taaatgcaaa cccgagaata acaatcaaga cgcctaaaag gctaagataa ttttccatat
    16321 attttcctcc ataaaatgac aatatttttt taattataaa gcaaaacaca taaaaaatat
    16381 agttgttttc tcacgaaata gacactattt ttaaatattt ttcgttattt ttttatcaca
    16441 aaaattatct tacttattgt aaggtaaagc tttttttaat ttattgcccg ataatatata
    16501 gaaataaggt ctagttactg ttttttttaa atgattaata aataacaact tctttctatg
    16561 aaattgcaaa gcattcatct tcttttccgc taacgatata tccgtcagtg cttggtacac
    16621 tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga
    16681 tatacaatct gccaccgggt aggataaaga ggatgatcat tccattaggt ctttgaaggg
    16741 atgattttcc tctttttgtg tattttttta tagttaagga ggaatgtaag tatggcatgg
    16801 atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt
    16861 gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggcatgat tgctagtttt
    16921 tactttttgt caaaggcaat caaaagtttg ccgatgagtc tctcttatcc tatctggaca
    16981 gggattggcg cagttggttc ggtcataatt ggtgtggtct tgtttcatga taagctgaat
    17041 ctttccactt ggttctttgt ggggatgcta ctcatcagta tcattggcat caagattact
    17101 agcggccact agacacttgg aataagtact gatcagaaaa aataagcctt tttatttaat
    17161 attcaatttt tttatgaaaa aataagaaat aagtccaatg acctagcgac ttctccactc
    17221 atcaggtgta tgcttgtact atctatagaa aaggagtgaa tatttttgaa aagtctttcg
    17281 gtccaagaat tatccaagaa ctttggtcac aaacaagccc tcagcgatgt cagttttagc
    17341 ttatcccctg gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa aacaacgatc
    17401 atgaaagcta ttttaggact gatccattac tcagatggaa caatctgtat tggtgaaaat
    17461 gagatttctc cctctagtca taaagggttg gaacaagtag gagcattgat cgaatacccg
    17521 ggtatctatc cattcttaac aggctatgat catctgaagt tattctctga aaccaacgat
    17581 gtatctgcta tcgacactat cgtgaatcag ctcaagatgg aaaagtacat tcacaaaaaa
    17641 gcgaaatcct actcgttggg aatgaaacaa aaattgggaa ttgctcttgc cttactgaac
    17701 catccgtcta tcgttatttt agatgagccg atgaacggtt tggatccaca agcaacaaga
    17761 gatgtgcgaa aaatcattac cgacttggct actcagggca cctctttctt gatctccagt
    17821 cacttactca gcgagcttga aaaaatggtc gatgagctga tcctgatcaa tcaaggaaag
    17881 atcatcaaac aatgtacaat ggctgaattg aaccaaacag cgcaagattt catcgttatc
    17941 tctacaacaa atgatctagc tgctcgaacg atcttgacag aagctggata tactttggaa
    18001 gatcctgata aaatcaagct tttaaaagaa aacgaagaat tattaggaac cgtgatccag
    18061 ctattgtctg cacaaaatat ccatgtgaca gacgtccagc atctgacgaa tgatttggaa
    18121 tcttctctat tggaaattct ggattcagta aagggggaag aagaaaaatg acattgcttg
    18181 taaaacaaga attatttaaa ttgatcaaga aaaagtcaac tgctgtttta tctgttctat
    18241 tagttgtttt gctcatagga acagcacttc tagccaaaaa atacacgacg atcattgatc
    18301 cagttgaaat gactgctcag ctttttagtg ccacatcttg gattgtcttt atcatgattg
    18361 cagctgcaag cacgatcatt tcgatggaag cacaatatgg tacattgaaa aatctccttt
    18421 atcgaaagta ttctcgagga gaaatattag taagcaaatg gatcacatta gttatttatt
    18481 ctgtttattt atacctttta gctattattg tcactgtgtt gatgaaactg attcttttcc
    18541 cttcgatttc cttcacggag aaggtttcta ctggtcaaac actgatccaa agcttgttca
    18601 cttatacttt gggtagttat atcggtttat ggctgatttt aagtttagtg ttgatgattg
    18661 cttgctttat caatagttcc ggcgcatcca tctcagcagg aatcgtcttt tattttgctt
    18721 cgtcaatcat atctggtatc cttattgcac tcatccaaaa atgggagtgg atcaaatgga
    18781 atccgatcag tatgttgaat ctgcaaaacc aaattggaaa cgaagagatc atgaagcaat
    18841 tgactcatct ttctactaat caaatgctat tcggtaattt agcttacatc gttttgttct
    18901 tagcattagg atatctcgta tttaagcgaa aaaatgtatg atgactcatc aaaaaagagg
    18961 ctgtgaaaga gacgttcagc cttattgccg attggctgtc tcttaaacac cgtttattct
    19021 gaaaaaggag ctgtgacaac atttgtgtca cagctccttt cctagtatgt ttttgaataa
    19081 acgacaggct cacaacctct ctttatactt cttttatact tctatacgtg aagcatccca
    19141 gtggcaaata aaaccattgc aatcactccg accatcgtca cacctataag taaaatctta
    19201 tctaagtgga tatgttgtac aggctcgttt tgctccttac gtgctttgag gtaaaaataa
    19261 atgcctggca agaaaagaac aagaacaagc attacttcat taaaggctac gactagcata
    19321 gcccatactt gaaaggcact tgctaaaagc cctactaaaa attggaccca ttctttccgc
    19381 tgataagaaa gtttcatttg gtacaatcct aagaaaagcc acgtgaagaa aatagctgct
    19441 gtaccaagtg tgtaagcaaa gttataagca ctgtcagtga ataataatga aagtaaaaag
    19501 acattcgtac ataacgtcgt caatacgata gcataagttg gtgccccatt tttgttgagt
    19561 tttccccatg ctttaggcaa aagtccgtct tcagccatgt ttcccattgt ttcaatcggt
    19621 aagatcgtcc atgagagcca aacaccaact aaagaaatga tcaaaccgcc gttgatcacg
    19681 actgctcccc atttaccaac catcatttca aacacatagc ccatagcagg ctgcgccttg
    19741 atcgtttgca gctgattcat cgtcattgtc ccatatggaa gcatggaaac caagacgtaa
    19801 ataataatca agccgaatat ccctagaatc gttgctttcc caacatctga ttgttttttt
    19861 gctcgtgaac ctacgacaga ggctccttca ataccaacaa atacccaaag catcaccatg
    19921 atgcaactca atacttgact ggaaaacggg atcggctgtc cattcgcatt caatataatg
    19981 tcgctagaaa agttatcaat gaattgttgg atcgaaaacg tgctgatagc tactacaata
    20041 aataaaaata atggcactaa tttacagaat gtgacgatag cattgacgaa actagcattt
    20101 tcgactcccc gattcactag ccatgcatat ccccataaaa agactgtact catcacgatc
    20161 gatgccagat tttgcccacc ttcaaatact gggaagaaat atcctaatgc gctcatcaac
    20221 aatgttgcaa aagctacgtt gcctaaccag catgtgagcc agtaacccca accgctgata
    20281 aaaccagcga aacggccaaa accttcttct gcgtaactaa agatcccgcc ttttaaatct
    20341 ggacgttttt tattgatatt attgatcgac aaggagagca tcaacattgc tgttcctacg
    20401 agtacccaac tgaaaatagc tgggccgcca gccgatccgg agatgtccgt cataatacca
    20461 aatacgccac ctccaatcgc tgaaccaatt actaaggcag caagtggaat taaaccaatt
    20521 ccttttttct gttgctctgt atccatgtaa gcatcctcca aaataaaacc gcttttatca
    20581 aaaagtataa caaacctttt ttatttttac ttaattaata aaaactttaa tagaaatata
    20641 attctaccgg cttttttctc attatacaag caaagcgcct agtccctttc caaattacac
    20701 caccaaaaaa cttaaaaaag atgctatact aaaaggaaag aattggagga tgatccatga
    20761 acattttata tatatcgatt tcaggaaata cgcgtgcctt tgccaaacat ctagcagaat
    20821 atgctgaaaa aatgcatgca gaagatcctg tcaatcctga agttacttta aaagaaatcc
    20881 atgaaaatag tgattttacg aaagaaacag aaccattttt tactttcgtt cctacttatt
    20941 tagacggcgg aaatggttta gataacggtg atacagaaat cttgaccgaa acgatgcgtg
    21001 aatatctgga acatgaagac aatcataaac tttgtcttgg tgtagtcggt agcggaaata
    21061 aaaacttcaa taatcagtat tgtctaacag ccaaacaata tgcacaaaca tttggctttc
    21121 ctttcttggc agattatgaa ttacggggaa caccatctga tgtgacacga atttatcaga
    21181 ttttagcaga aaacaataaa taataaaaaa gaagaagctg aaaaatagat ttcagtttct
    21241 tctttcgttt ctattgtttc tatcatatta agctttgaca tccatcacag gtgcttttgc
    21301 ttttgcttta cctgtttttt ccaagacaaa gttttttaca cgatccatat tggttaccaa
    21361 gacgatcatg gatgtttctt tgcctgctgc tttgatcgct tctaagtcag cttctgctac
    21421 aagcgtatca ggtgtcactt gctgcccttc tgttactttc acatcaaatg gcgtaccgcc
    21481 taagtcaact gtgttgattc ccatatgaag caagatttct aaaccatttg gcgttgtgat
    21541 cccaatagcg tgtttcgttg ggaagacaga gctgactacc ccagtaactg gagaatagat
    21601 atgtccgttt tctggttcta caaagtagcc ttcacccatc atcttttgtg caaaaactgg
    21661 atcacttgct ttatctaaag aaacaactgt tccgtcagca actgatgaga ggatagtcac
    21721 actttcagct gaagtttgag gtactacttt ttcaggcact tcatttactg tggctgtttc
    21781 agtcgctaat tcttcttttt ctaaacggtc tttaggaata ccgaagaaga atgtagcaac
    21841 gaagccacca gcataagctg ccaataatcc aaggatgtag cctaaccatt taccgtcaga
    21901 gatcaatggg atcaaagcgg ctcctgaagg tccaatagcg atagccccga catttccgat
    21961 cattccgatc actgccccgc cgatacctcc accgatacaa gcagtaataa atgggcgtcc
    22021 tagaggtaat gttactccgt aaatcaatgg ttcgccgatt cctaggatcc cgactggtaa
    22081 agctcctttg atcatttctg ttaattcttt atcttttttg cagcgaatcc ataatgccaa
    22141 tgctgcaccc acttgtcctg caccagccat cgcaagaatt ggaagcaata gcgtacgacc
    22201 agtttcatca atcatttgca catggattgg agtcaaaatt tgatgcagtc cgaacataac
    22261 catcggcaag aataatgtgc ctagaacgaa gccggcaaat gctccaccaa cgccaagtac
    22321 ccagttgatc ccaccgacca tgccatcaga aatgaaacct gctaaaggca tgatgaagaa
    22381 aatttcaatc agaccgatgg caatcaatga aagagttggt gtaacgatga tatctaccgc
    22441 atcaggtact actttatgca gttttttctc aacgattgac aatagccaaa cagcgaagat
    22501 gacacctagg atcccacctt gtccagctgc aagagcttca ccagtaaata agtttttcag
    22561 tggtgcttcc ggattcatcc ctgtcagcat cacaacggcc ccgataactc caccaagagt
    22621 tggtgttgca ccaaaaactt gtgcagcatt gatacctgta tagataacta ggtagctaaa
    22681 catcccgttt ttcaagatgt tcatcacatc gatatattgt tgccatgttg ctgcatcaag
    22741 tgttccggct gtgactaagt ttgctaaaac tgcggcaatc cctgccacga tcccagtacc
    22801 gacaaatgca ggaatcattg gcacaaagat attagaaatg tcttttaata ccgatttgat
    22861 tttagatggt ttttgtttcg atttttgtgc cgctttcatt tcttgtgccc gttcgtttac
    22921 acggtctttt ccagatgctg atgaatttgc tgtagctgta cttacagaag aaagtttttc
    22981 tcctaatttc acgcctgcca aatcaaccat ttgctgtgct actttgttca ctttcccagg
    23041 tccgatgatg acttgaagct gttcatcttc tactacacca agcactcctg gaatcgcttt
    23101 taattcagag acatttacaa gatcatcatt gatgatcttc atacgcacac gagtcatgca
    23161 gtggacaatg ctttctacat tgcccatgcc gcctgcccct ttataaattt gttttgccag
    23221 ttcttccaaa gtcagttcgt tttttgccat ggtcttttcc ccctcataag gtccctttta
    23281 caaatccgtt tgcttcagct aattttgctt gagcttcctc tttatcacta tttgtcaaaa
    23341 tcatgacgat agccagctta acattttgat ctgcttcttc aaagtacttt tcagctgttt
    23401 cgtaatcagc ttctgtcgct tgcatgatga ttcgttttga gcgttcgatt agtttttcat
    23461 ttgttggttt gacatcgacc atcaagttat tgaatacttt acctatacca atcatactga
    23521 tagtagaaat catattcaag ataagttttt gtgctgttcc tgacttcaat cgagtagagc
    23581 ctgtcaaaaa ttctggacct gcatcgactt cgattgggaa atccgcgtgt ttactgattt
    23641 cggcttgttt attgcaagaa atcgttcctg tgacagcacc gatcgatttt gcgtaatcta
    23701 atccaccgat cacatatggt gtacgaccgc tagctgcgat accaataact atatcatttt
    23761 ttgtcaaatt cagcttgatt agatcttctt gccctagctc ataagaatct tcggcccctt
    23821 cgacagctac agtcattgct tcttctccac cagcaattag acctatgacc atttccggtt
    23881 ctacaccgaa tgtcggtacg cattcagcag catctaacac acctagtctt ccacttgttc
    23941 cggctcccat ataaatcaga cgtccacctt ttttaaagga ctcaatcgtg ttagcaatca
    24001 ctggttcgat acttgtcagt tctttttcaa ccgcagcggc cactttctga tcttctttgt
    24061 tcattagttg aacggcttga gaaatactca tctcatctaa tccaaaagtt tcttggtttc
    24121 tgcgttccgt tgttaggttc tctaaattga ttttgctcat ttttatcttc cttcttttct
    24181 tataaattga tagttcatac cagctttgat ttggttgatc aatggtagct cttctttgat
    24241 aactttagct actacattca ctttttcatc cgctggcaaa tcatatttgg ttaattggat
    24301 ctcacccata tagcgcaaat acttttcgtt atctaaagta atagaccctt tcatacgcgt
    24361 agctgtattt cttgctggga taggcggaat ctctcgaaaa cgtgcatcta cgcttcgtat
    24421 gacatctctt gcatcgtctt gccgattcgt atgcttccct aatatatatt cgtaaaattc
    24481 ttcatctaca ggttccgtat ggagcaagac tgtctgttct ttttggaaag aagcaaattg
    24541 ctcctgaacg ttttctgaca gacctgcatc tccgatgtaa acaatatccg tgttacttgc
    24601 taatagatct agagcgcctg caagaggatg gatccctcga tgttcttcca aagttggcaa
    24661 tccttgatat agtgggcctc tcaactctgt atctcctggt acaaaacctt ggacaaggag
    24721 accttgaatt ttgagccatt gatttttttt ctgataccat tctttgtcca gccctgtttc
    24781 gggacgtgga taataattat gccaagcttc aatctgtgag aaatcagctt ctgcttcttt
    24841 caattcatcg atatctttag gcgtgatcgt gctagcattc aaactgattt tcaattgatg
    24901 tgaccagtcg gcaatctgct gattggaaat atgataatcc attcttaaac ctgttacacc
    24961 aattgctttc aaaggcgtta gatcttctaa tgaaaaaccc gctcgttcca atgcctctcc
    25021 tgaaatatcg accatcaatt ctagtttatt ctttttcgct tcgcttccta gttcgatcaa
    25081 tcgcttttga tatgccgaaa catcatcttc tggtatatgc atggaagtga aaataccgac
    25141 aaagccattg gatgccattt ggcggatata ctgtttcttc tcttctgaca aagaatcgtt
    25201 cataaatatt gaaaagccaa acatattatc cctctttttc tgtatccgtc ttcatttcta
    25261 tgactagagt atatatctaa agaaacaaaa tttcaatact tttattaaaa taattaaatt
    25321 tcatttctaa aggttgagtt tatattatca aaagaattgt cataacctaa aaaaggcact
    25381 gctaagcaaa agcagtgcct tgaaatattt tatttaaata acacaaaaac ataattactc
    25441 ataagaaacc aattctgtat tatctaccat gaatgtaaag aacccctcat taggcatcgg
    25501 atgaaaataa gcattgatca ctcttatcaa ggcattatgg caaactaaca gatagacatt
    25561 ttcttcgtct tcgatgcatt ctttcaataa aggaacgatc cgtgcataca catctaagac
    25621 tgactcccca tttgggaatc gtacagcgaa cgctaaacgt gctttttgaa agttctcatc
    25681 tttgcttggc agtccatcat aatccccaaa atccatttct accaatcgtt catcaacggt
    25741 cagcggcaag ctgagacgat cagcaactgc ttgtgctgta tctcttgctc gttgaagagg
    25801 ggaatggatg attttcgtga ttggaacagg caaatctact accttttctg ctagtttctg
    25861 tgcctgcatc tgtccttttt cagtcagcgg cacatctgcc cgtccgcaaa ctaatccttg
    25921 tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc atgtgattct ctccttcgtt
    25981 ggcaaagttc cccttaacca taccttgttg ttcttgagat tacaagggtt agattctctc
    26041 aagagaaata atctcagtag atactttatc caggaaggtt tgatcatgtt ccactagtaa
    26101 catggcaggt ttcacgttca agatcagttg ttctaattgt tcttgattga agacatccaa
    26161 ataattcaat ggttcatccc atgtatatag ttcagctggc tgtgataaag atttagccaa
    26221 ttccactttt ttccgttggc ccatactcat ctgctcgatc ttgttatgaa aaacatctct
    26281 ttccatccca agctttcgga ggttgttcaa aaatgcttgg tagtctactt ggtttttctc
    26341 tgcaaattcc gccaacgttc ctcgattgtc ttcataattt tggcttgcat aactaatgct
    26401 cagatgtttt ggctggtatt tttctcctat aaccttgccg ttgaatgccc ccagaagata
    26461 atggataatg gatgactttc ctgcaccgtt aggacctgaa atcgccaccc gctgatgagg
    26521 ctcgattgta aaatgaattg gctcgaataa cagattttca taccctaatt gaagatcttc
    26581 tacgcttaaa agtcgcttat ggtgagacgc ttggctattc atcgtcaaag aatcgatata
    26641 ttcgatatct tttaatagtt tttctttctc gctgatctgg gtctccatcc gattcacgat
    26701 tgctttggat cgtttcatcg tccgtgcagc atcagcaccc actgctcctt tattcactcg
    26761 tctagattca gtatcgatga atccgacttg tttctttgtt ttatctcctt ctcgggaacg
    26821 agaccattcg gctttttcag ctgctgtttt ctttagccta ctgacttctt ttttcaattt
    26881 ttcattttga gccatttcaa attcatcacg aagttttttc tgttcttcat agatagagaa
    26941 attcccttga taaagttcca gttgactttt ttcgattgct aaaacatggt ccactacttc
    27001 atcgataaat ccccggtcat ggctgaccac gatgaagcct tgttttttct ttttcaaata
    27061 agccgctact tgttttctac cagagatatc caaatgattc gttggttcat cgattaacgg
    27121 gaaatgagtg tcatccacaa ataaaagtgc cagtaggact tttgtcttct ctccccccga
    27181 tagtgtgctg aattctctcc ataagatttc aggatctgtt tgcatcaatt ggagctctct
    27241 ttcgatttcc catatctcaa aatccgtaat atcatttaac acgtaatagg ttaaacgttc
    27301 tttatctttt gtctgttgcg ggaaataggc aaattcttgc tgatggatta cttgcccttg
    27361 ataaggtagt ttgttttgta gaatattcag taaagttgtc tttcctcgac cgtttcgtcc
    27421 gataagtcct agtttccatt gtgtgtcaaa atttagattt gcttgttcaa ataataatgt
    27481 gccttggctg tcgtagccga atgtcagatt ttttatttcg attttagaca ttttccatca
    27541 cctcgtaaaa aaagaccagt cctattttca gaactggcta aagaggatgc aagggttgga
    27601 tacacacaaa aagacccata catcgaaaaa accaagccaa tcccgaaaat ctgcacactg
    27661 cttccttgtc tccacaaggt acagtctgaa tgcagctttc atcaatcgac ttaatttttc
    27721 aatgcacgga tccctcactt tgtcctatat ttgaaattac gatagcataa agaaaatgat
    27781 tttgtcaact acttcgggag gatggtgaaa aatctgctaa gtgtctgtcc taagattttt
    27841 gttttttcta atgtttgcct gtgttccatt gcttcttcta aacctattgg acccaaatga
    27901 atacttaaga caattgggag aaaatcagaa agtccttcta gctcttggtc tctacttccg
    27961 caaatagcga ttattggttt tcgatatttt tttgcaagca ctgctacacc gtatggaact
    28021 tttccgtgga tcgtttgctg atcgatccgc ccttctcctg taatgatgac atctgcttgt
    28081 tcgatctttt tttccaatcc agtgatttgg ctgactaaat cgaaaccaga aaccatcttc
    28141 gctcccaaca aaaaaagggc tccaccaata ccgcctgctg ctccacttcc tggaatatca
    28201 tctaaatcca cctcccagaa atcttttatt tgctgagcaa tctttcctgc ttgtttatct
    28261 aagaaagcta gcgtcgtatc tattcctcct ttttgtcttc caaattgcca agcagctcct
    28321 tgttgacctg tatatggatt ggtaacatct gcagcgataa tcaattttac tttagaaaca
    28381 agaacgtttc ttcgatcaaa atgaagtttt ttagtagata ataaaggatt tccttctgtg
    28441 tacccttcta aagaagcacc caaggattgg agtaatccta gaccaccatc gctacacccg
    28501 cttcctccta aagtgacaat aatttgttct acttcttgaa gcagtgcatc tttaataagt
    28561 tctcctaagg cataactgga agcaagtcgg atagtttgat ccgttggttt atcaagtaga
    28621 tctaacccca atgcttgtgc agattcgatg actgcggtgt tttttccttg ccaagttgtc
    28681 aataaatagc tgatttttcg ctgacggaat aagagatcca tcccatcaat ggtacgccat
    28741 gttcccctga gcgcgtaata gattgcttct gcagtatttt ctccgccatc tgcaatcggc
    28801 acattgattt ttcttccttt acgatcttcc cagccattta gaactgcctg atttagttca
    28861 aatgaagaag cacttccttt aaatgaatca attgccgcca aaatatccat ctgtatccct
    28921 cactttcttc ttaagtaaga cacttttttt catttttttc aaacaaaaca atgctatttt
    28981 cttccatttt tctatatact aaaagggaac tcattgaata aaggagagaa aaaacatgaa
    29041 aatcgcaata gctggggccg gagcaatggg cagccgaatc ggattgatgc tacatcaaag
    29101 cggaaatgag gtcctcttga tcgatcgttg gcctgctcac attgaagcca ttcgtacaaa
    29161 tgggttgatt gctgacttta acggtaaaga agtggtcgca aaactgccaa tctattcacc
    29221 agaagaaatc atcgaaagca acgaacacgt ggacttgatc gtagcattaa caaaagccaa
    29281 tcaattagat gatatgtttt gttcgatcca atccattatt actgacaata cttatgtttt
    29341 gtgtttacta aatggattag gtcatgaaga tgtactggaa aaatatgtgc caaagaaaaa
    29401 tatcttattt gggatcacga tgtggacagc tggtctagct ggtcccggaa aagtcacatt
    29461 gttaggtgat ggagaaatcg aactagagaa cttggaacca gaaggcgaag catttacaaa
    29521 aaaagttgta gaagtctttc aagaagcaaa tttaaatccg atctatagcc ataacgttcg
    29581 ttattccatt tggcgaaaag cttgtgtaaa tggaacattg aatggtttat gcacgatttt
    29641 ggattgtaat attgctgaac taggcgcgca aaaagcggca gagagcatgg ttcgaacaat
    29701 cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc atactagatc aagaagaagt
    29761 gtaccaacat attgcttcta cttatgatcc tgacaacatc gggttgcatt acccatctat
    29821 gtaccaagac ctgatcaaaa accatcgttt gactgaaatc gactacatca atggtgccat
    29881 ttggcgaaaa ggacaaaaat atgatattgc tacaccatat tgtgcctttt tgacccaatt
    29941 agtccatgca aaagaaggaa tccttggcgc agaataaaag gctctctgat gagaatcagc
    30001 tgaatcagca gctggttctc tttttttaag cccaataaaa gatcaaaaag aaaacacaac
    30061 tctcatgatg ccataagaat tgtgcttgtt tgtttcagta cataatctct tcaaaatgta
    30121 ggttttttat ttgttttcaa cagaggagag gtcatattcg tattgctcat aaatagtgga
    30181 atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca taaaattcca atagctctga
    30241 taggattggc ttaaatggtg tatgcagaaa ttccggtaat tcatctaaat cacgttctcg
    30301 aagcaaaaaa atattttcag gacgataaaa atgataatga gcaatcgctt gattatccgt
    30361 cactagtttc ttttgaaaat acatggattc aaatgtccgc aaagtcactc cttgctgacc
    30421 ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt tttttcaggt actcttcata
    30481 agtaagatat ccaggttgtt cattccctcg atgtggaaga atgaaatagt tggtcgtaac
    30541 atctcttttt ataaattcct gcttatagct ttctgctcgt tcttttctcc cattattcgt
    30601 tgcaccaaaa aatagatccg tctttggttc gctagaagaa aaattcatat gtttcgaata
    30661 aaatgaacta ttatgtttca gcccaagttc ttttgcttcc aaaaaatcaa aatgataaat
    30721 ctcatcgatt gccggttcat cttgtgcctg cttcagcatc gcctcgtgtt ctgctttgaa
    30781 gtgattccaa aaaaataaca caatttttgc attggtaaat gggcgcacgc gtcgagccat
    30841 ctccatatca aatccataat ctaaaaaaac gatcagctga taatttgcca actgttgtct
    30901 agctgattta atgaattccg actgtagtaa atcttttccg gaccaaacat ctaaagagcg
    30961 gatggaaggc aaaaaataaa gtgatttatt ttttgcaact agcagtacgt catttttgtt
    31021 catcttttcc gcctcacttt ctctgtcact ttttcctatg atagcctgtt taaatagtaa
    31081 a
//
LOCUS       DACCIQ010000025        30731 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCIQ010000025 DACCIQ010000000
VERSION     DACCIQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.025"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="IVM62_001187"
     CDS             complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="IVM62_001187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP6845156.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="IVM62_001188"
     CDS             complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="IVM62_001188"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6845157.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8109..9077
                     /locus_tag="IVM62_001189"
     CDS             8109..9077
                     /locus_tag="IVM62_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6845158.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9095..10000
                     /locus_tag="IVM62_001190"
     CDS             9095..10000
                     /locus_tag="IVM62_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845159.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9997..10803
                     /locus_tag="IVM62_001191"
     CDS             9997..10803
                     /locus_tag="IVM62_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6845160.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10960..11928
                     /locus_tag="IVM62_001192"
     CDS             10960..11928
                     /locus_tag="IVM62_001192"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP6845161.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12245..12769)
                     /locus_tag="IVM62_001193"
     CDS             complement(12245..12769)
                     /locus_tag="IVM62_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP6845162.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13056..14867
                     /gene="scm"
                     /locus_tag="IVM62_001194"
     CDS             13056..14867
                     /gene="scm"
                     /locus_tag="IVM62_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6845163.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15022..16008)
                     /locus_tag="IVM62_001195"
     CDS             complement(15022..16008)
                     /locus_tag="IVM62_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6845164.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16031..16597)
                     /locus_tag="IVM62_001196"
     CDS             complement(16031..16597)
                     /locus_tag="IVM62_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP6845165.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16611..17027)
                     /locus_tag="IVM62_001197"
     CDS             complement(16611..17027)
                     /locus_tag="IVM62_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP6845166.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17484..19418)
                     /locus_tag="IVM62_001198"
     CDS             complement(17484..19418)
                     /locus_tag="IVM62_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6845167.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19720..20655
                     /locus_tag="IVM62_001199"
     CDS             19720..20655
                     /locus_tag="IVM62_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP6845168.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20745..21848
                     /locus_tag="IVM62_001200"
     CDS             20745..21848
                     /locus_tag="IVM62_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6845169.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21861..22814
                     /gene="menA"
                     /locus_tag="IVM62_001201"
     CDS             21861..22814
                     /gene="menA"
                     /locus_tag="IVM62_001201"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP6845170.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23749..25095)
                     /gene="gor"
                     /locus_tag="IVM62_001202"
     CDS             complement(23749..25095)
                     /gene="gor"
                     /locus_tag="IVM62_001202"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP6845171.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25229..26470)
                     /locus_tag="IVM62_001203"
     CDS             complement(25229..26470)
                     /locus_tag="IVM62_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6845172.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(26488..27411)
                     /locus_tag="IVM62_001204"
     CDS             complement(26488..27411)
                     /locus_tag="IVM62_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6845173.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27632..30124)
                     /locus_tag="IVM62_001205"
     CDS             complement(27632..30124)
                     /locus_tag="IVM62_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6845174.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30131..30598)
                     /locus_tag="IVM62_001206"
     CDS             complement(30131..30598)
                     /locus_tag="IVM62_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP6845175.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8891 a         6535 c         5746 g         9559 t
ORIGIN      
        1 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
       61 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
      121 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
      181 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
      241 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
      301 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
      361 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
      421 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
      481 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
      541 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
      601 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
      661 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
      721 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
      781 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
      841 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
      901 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
      961 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
     1021 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
     1081 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
     1141 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
     1201 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
     1261 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
     1321 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
     1381 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
     1441 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
     1501 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
     1561 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
     1621 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
     1681 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
     1741 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
     1801 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
     1861 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
     1921 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
     1981 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
     2041 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
     2101 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
     2161 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttgg gaaggagtaa
     2221 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
     2281 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
     2341 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
     2401 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
     2461 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
     2521 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
     2581 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
     2641 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
     2701 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
     2761 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
     2821 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
     2881 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
     2941 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
     3001 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
     3061 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
     3121 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
     3181 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
     3241 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
     3301 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
     3361 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
     3421 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
     3481 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
     3541 cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
     3601 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
     3661 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
     3721 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
     3781 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
     3841 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
     3901 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
     3961 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
     4021 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
     4081 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
     4141 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
     4201 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
     4261 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
     4321 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
     4381 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
     4441 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
     4501 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
     4561 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
     4621 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
     4681 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
     4741 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
     4801 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
     4861 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
     4921 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
     4981 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
     5041 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
     5101 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
     5161 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
     5221 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
     5281 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
     5341 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
     5401 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
     5461 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
     5521 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
     5581 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
     5641 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
     5701 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
     5761 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
     5821 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
     5881 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
     5941 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
     6001 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
     6061 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
     6121 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
     6181 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
     6241 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
     6301 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
     6361 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
     6421 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
     6481 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
     6541 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
     6601 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
     6661 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
     6721 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
     6781 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
     6841 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
     6901 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
     6961 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
     7021 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
     7081 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
     7141 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
     7201 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
     7261 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
     7321 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
     7381 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
     7441 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
     7501 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
     7561 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
     7621 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
     7681 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
     7741 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
     7801 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
     7861 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
     7921 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
     7981 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
     8041 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
     8101 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
     8161 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
     8221 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
     8281 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
     8341 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
     8401 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
     8461 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
     8521 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
     8581 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
     8641 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
     8701 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
     8761 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
     8821 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
     8881 acggcggtct agcaacagta ggaatcgact acgaaagctt gggtaaacaa acagggatca
     8941 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
     9001 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
     9061 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
     9121 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
     9181 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
     9241 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
     9301 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
     9361 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
     9421 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
     9481 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
     9541 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
     9601 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
     9661 gatttctaat ggatgcattg ctctttctgg ctccttgctt gcgcaaaaca acggctttgc
     9721 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
     9781 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
     9841 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
     9901 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
     9961 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    10021 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    10081 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    10141 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    10201 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    10261 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    10321 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    10381 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    10441 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    10501 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    10561 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    10621 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    10681 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    10741 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    10801 taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct
    10861 tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc
    10921 ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta
    10981 cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg
    11041 gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt
    11101 tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa
    11161 atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct
    11221 gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta
    11281 tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa
    11341 cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg
    11401 cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt
    11461 cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg
    11521 attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg
    11581 atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc
    11641 atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc
    11701 tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa
    11761 aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt
    11821 cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg
    11881 agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc
    11941 acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct
    12001 ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct
    12061 ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt
    12121 tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg
    12181 taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag
    12241 tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag
    12301 cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct
    12361 cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat
    12421 cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg
    12481 cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc
    12541 tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat
    12601 ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat
    12661 cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc
    12721 gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc
    12781 ctttttattt atcctactta ctttttccta attctaggat acctacttat atgttactac
    12841 acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa
    12901 aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat
    12961 tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata
    13021 ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag
    13081 tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat
    13141 acattcctaa agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag
    13201 gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag
    13261 acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg
    13321 aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt
    13381 caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg
    13441 tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag
    13501 atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta
    13561 aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg
    13621 cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt
    13681 ttttattggt aaatatcgat gcagaagtgt taacgatgaa tacgccactt caaatcatta
    13741 atccaaatga tcaagtagtt gtatttaatg tcatcaacag ttcttctgca ttgaacgtac
    13801 agtcaccaat caaatacaat gatcgttcaa atcatgaaac agaagatttt tcggatgcga
    13861 atatctcttg gaattttgga aatgagatga ctgatttgac aatcagtgct ccttttcaag
    13921 gaacaatcct tgcgcctaat gctactatcc gcattactca aaaccaagac ggaacgatta
    13981 taggaacaaa tgtgatttta gatgcagcta ccaatcgttg ggatcctaac gaaatattta
    14041 tcagcgacaa tggtacagat acgacagaca caagcgacag tacagataca tctaccacaa
    14101 gtgatagctc agattcatca acttcaagcg acagtacaga tactacctct accacaagtg
    14161 atagcacgga tacatcagct tcaagcgaca gtacagatac tacctctacc acaagtgata
    14221 gcacggacac atcagcttca agtgacagta cagatactac ctctaccaca agtgacagca
    14281 cggacacgtc agcttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
    14341 acacatcagc ttcaagtgac agtacagata ctacctctac cacaagtgat agcacggaca
    14401 cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc acggacacgt
    14461 cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca gattcatcta
    14521 ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat acatcctcta
    14581 ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg tcgtctacca
    14641 caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat aacaggacga
    14701 actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa agagcacttc
    14761 ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata ttacttgtaa
    14821 tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa aatattcaag
    14881 ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt tatcataaat
    14941 acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag aaatttattt
    15001 acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg taagggaaaa
    15061 gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct tcaaagcctt
    15121 ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat gttcaacgat
    15181 tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta aataaggtag
    15241 tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga ccccttgacg
    15301 catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat ctaaaatatc
    15361 atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt tttttgcgaa
    15421 gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga acagttcagc
    15481 tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat tatagcgatc
    15541 atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat tcaattggag
    15601 acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa ataaatagtc
    15661 tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct gaccacgtcg
    15721 cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga gcatttcgat
    15781 agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa
    15841 tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt tgattgcttg
    15901 ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg tttctgtcaa
    15961 ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa aaaaactcct
    16021 tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt ccctgctcat
    16081 aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc gctgcgatcc
    16141 cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac aacatgactg
    16201 tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc atgaaaccgc
    16261 cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg accaataaca
    16321 ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa atcgttccgc
    16381 ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct cgttttttca
    16441 tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca ccaggcgcaa
    16501 aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct tgtgcaacaa
    16561 gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc
    16621 agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt atgtggcaaa
    16681 cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg
    16741 caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt ataatctccg
    16801 tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg gaatgttttg
    16861 atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt gttttgctga
    16921 agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat aataaaaaca
    16981 tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt tgttctcctt
    17041 tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca cctgtgtctt
    17101 gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta attctttggg
    17161 cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt gacagaagtg
    17221 ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt ttgtgaaatt
    17281 tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga ggttgtaaca
    17341 gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt attcctttaa
    17401 ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg aaatcttgca
    17461 ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg tctaccgtac
    17521 caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa cccaatcgca
    17581 cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt tacccagtaa
    17641 aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact gcatagccat
    17701 gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac catgaatttt
    17761 tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt tgtgaaccag
    17821 tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact caaaccaaaa
    17881 tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc agctgtggtt
    17941 gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc ccatgtccaa
    18001 gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact tggtttgaac
    18061 acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa accatagaaa
    18121 atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc tcgagtcacg
    18181 ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat ataataacca
    18241 gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat agctaaaaat
    18301 ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc aatagaaacc
    18361 ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat cgaagccacg
    18421 atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt
    18481 gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt tttatcttcg
    18541 tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc tgattctaat
    18601 ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag tgtgtgagtc
    18661 ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac gattggtgca
    18721 ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc agcatcattt
    18781 cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt gatttcgttt
    18841 ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc cccaatcatt
    18901 tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt acgcagttca
    18961 tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg aatccttaca
    19021 gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt accaaagtca
    19081 ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc taatttggtc
    19141 ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag ttgtacattc
    19201 ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc tacacgtccg
    19261 ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc gatcagagta
    19321 attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga aacaccggca
    19381 tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct ccttatagac
    19441 tatctatata atgaatatat gataattctt ttagtttgat acttattaca caatctccat
    19501 atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt caaattttaa
    19561 aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt acatgggtta
    19621 tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg aatcacaaag
    19681 aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta
    19741 actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg cgcagatgac
    19801 aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac tgcaaaaagc
    19861 acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg cacatataaa
    19921 ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac
    19981 ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa
    20041 gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga aaaatacatt
    20101 cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact
    20161 ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat ccaagctgct
    20221 caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa agacggcact
    20281 tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga aatgacagtt
    20341 gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg taaatcaaaa
    20401 caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact
    20461 tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct
    20521 gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat catgtacgca
    20581 caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt agataatatc
    20641 gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg acgatagcca
    20701 gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac
    20761 tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc agcaactcag
    20821 caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat ccatattctg
    20881 atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat
    20941 cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc acgatcaatc
    21001 aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt
    21061 ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat tctcaaggcg
    21121 gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt gatgacgcca
    21181 gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat cataagatcg
    21241 aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc atcgatttag
    21301 gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa aaacaaggag
    21361 tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac agcccaagag
    21421 gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc ggatctgttt
    21481 taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac gagcgttatt
    21541 tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt tatccgttcg
    21601 acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt gacgggttgt
    21661 ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga
    21721 ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg accgatccaa
    21781 tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac cgttctgaac
    21841 taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt gaaatacgaa
    21901 caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc gctttttttc
    21961 atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt tttgatttag
    22021 cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa gtatataagt
    22081 atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga aacatgattt
    22141 tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat acgggttggc
    22201 ttttactttt gatgggattt atctgttgtt ttattggagt attctataca ttcggaccga
    22261 ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa
    22321 tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat ctgacagtca
    22381 acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca gcaatcgtat
    22441 tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat aatttgcggg
    22501 atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc ggacgtcctg
    22561 cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt ttaatggggt
    22621 tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg ccaattgtga
    22681 agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc agctattcca
    22741 tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta acaatcatta
    22801 tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc aagtaataag
    22861 aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat taccgcagat
    22921 gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt
    22981 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgag
    23041 aaactggctt ttgaacaccg tttatcagag gttgtgaaaa agtcgcttag tttcgagtat
    23101 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
    23161 gaaactggct tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta
    23221 ttagagaaac aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
    23281 aggacagctg attgaacact atttgttcgg aaaaagtgac taactatgac acaatttttt
    23341 tcacagtccc ttttttcact tgatttgatt cgtaccagcg aacttgacga gaactttctt
    23401 ggtttggtaa ctatatggta ttcttttaac atcctcgttt gcttcttcta atttatttat
    23461 acaaagtcac acttttttaa acatctgttc ttcgatacat ttctatttct tttaaacatc
    23521 gataccaata ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt
    23581 tacgaagata atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc
    23641 aatcttacat aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt
    23701 tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt
    23761 tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt
    23821 tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat
    23881 agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt
    23941 ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa
    24001 ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg
    24061 tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc
    24121 attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa
    24181 tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc
    24241 tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc
    24301 ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt
    24361 ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc
    24421 gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc
    24481 aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccacg
    24541 aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg
    24601 taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt
    24661 cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc
    24721 gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg
    24781 attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct
    24841 ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc
    24901 atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca
    24961 accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc
    25021 atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta
    25081 atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac
    25141 gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc
    25201 ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt
    25261 tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg
    25321 attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac
    25381 cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct
    25441 gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg attttacgac
    25501 gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt
    25561 ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag
    25621 gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat
    25681 ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg
    25741 gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc
    25801 ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc
    25861 agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca
    25921 atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg
    25981 tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga
    26041 cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt
    26101 gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg
    26161 gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg
    26221 ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat
    26281 gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc
    26341 ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg
    26401 tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt
    26461 ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa
    26521 tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc
    26581 cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg
    26641 agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta
    26701 atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat
    26761 atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga
    26821 gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt
    26881 ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac
    26941 aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag
    27001 tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa
    27061 tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat
    27121 aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata
    27181 ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt
    27241 tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc
    27301 aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag
    27361 caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc
    27421 ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta
    27481 aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc
    27541 caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta
    27601 ttataaaaat tcagctacac aggccaagta actataggga acctactgtt tcctttttag
    27661 gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta
    27721 aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg
    27781 cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga
    27841 ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt
    27901 tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag
    27961 tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac
    28021 gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt
    28081 cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt
    28141 ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat
    28201 tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt
    28261 gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc
    28321 cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg
    28381 catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac
    28441 cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc
    28501 gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt
    28561 catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta
    28621 gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag
    28681 aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg
    28741 ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt
    28801 ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat
    28861 cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg
    28921 gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat
    28981 ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt
    29041 ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag
    29101 aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt
    29161 ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac
    29221 ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga
    29281 tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa
    29341 ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac
    29401 ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac
    29461 ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt
    29521 ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac
    29581 gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta
    29641 catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt
    29701 tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca
    29761 tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac
    29821 cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg
    29881 gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt
    29941 cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat
    30001 tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc
    30061 gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt
    30121 ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc
    30181 gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca attgcactca
    30241 acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag
    30301 caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt
    30361 gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc
    30421 tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg
    30481 aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat
    30541 tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct
    30601 tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa
    30661 gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac
    30721 tattccgtat c
//
LOCUS       DACCIQ010000026        30406 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCIQ010000026 DACCIQ010000000
VERSION     DACCIQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.026"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            119..3397
                     /locus_tag="IVM62_001207"
     CDS             119..3397
                     /locus_tag="IVM62_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP6845176.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3938..5845)
                     /locus_tag="IVM62_001208"
     CDS             complement(3938..5845)
                     /locus_tag="IVM62_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP6845177.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5975..6850
                     /locus_tag="IVM62_001209"
     CDS             5975..6850
                     /locus_tag="IVM62_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP6845178.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6930..7904
                     /locus_tag="IVM62_001210"
     CDS             6930..7904
                     /locus_tag="IVM62_001210"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6845179.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8093..9229
                     /locus_tag="IVM62_001211"
     CDS             8093..9229
                     /locus_tag="IVM62_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6845180.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9366..9710
                     /locus_tag="IVM62_001212"
     CDS             9366..9710
                     /locus_tag="IVM62_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP6845181.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9981..11267
                     /gene="efmA"
                     /locus_tag="IVM62_001213"
     CDS             9981..11267
                     /gene="efmA"
                     /locus_tag="IVM62_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP6845182.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11405..12004)
                     /locus_tag="IVM62_001214"
     CDS             complement(11405..12004)
                     /locus_tag="IVM62_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP6845183.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12408..13529
                     /gene="mnmA"
                     /locus_tag="IVM62_001215"
     CDS             12408..13529
                     /gene="mnmA"
                     /locus_tag="IVM62_001215"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP6845184.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13775..15196
                     /gene="arcD"
                     /locus_tag="IVM62_001216"
     CDS             13775..15196
                     /gene="arcD"
                     /locus_tag="IVM62_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP6845185.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15210..16328
                     /locus_tag="IVM62_001217"
     CDS             15210..16328
                     /locus_tag="IVM62_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP6845186.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16388..17680)
                     /locus_tag="IVM62_001218"
     CDS             complement(16388..17680)
                     /locus_tag="IVM62_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6845187.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17830..18390
                     /locus_tag="IVM62_001219"
     CDS             17830..18390
                     /locus_tag="IVM62_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6845188.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18519..18896
                     /locus_tag="IVM62_001220"
     CDS             18519..18896
                     /locus_tag="IVM62_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP6845189.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18829..20901
                     /locus_tag="IVM62_001221"
     CDS             18829..20901
                     /locus_tag="IVM62_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6845190.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21112..22059)
                     /locus_tag="IVM62_001222"
     CDS             complement(21112..22059)
                     /locus_tag="IVM62_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6845191.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22305..22769
                     /locus_tag="IVM62_001223"
     CDS             22305..22769
                     /locus_tag="IVM62_001223"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP6845192.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22831..23874)
                     /locus_tag="IVM62_001224"
     CDS             complement(22831..23874)
                     /locus_tag="IVM62_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP6845193.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24251..25216)
                     /locus_tag="IVM62_001225"
     CDS             complement(24251..25216)
                     /locus_tag="IVM62_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP6845194.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25450..26295
                     /locus_tag="IVM62_001226"
     CDS             25450..26295
                     /locus_tag="IVM62_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6845195.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26486..27673
                     /locus_tag="IVM62_001227"
     CDS             26486..27673
                     /locus_tag="IVM62_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6845196.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27679..28035
                     /locus_tag="IVM62_001228"
     CDS             27679..28035
                     /locus_tag="IVM62_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP6845197.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28037..28375
                     /locus_tag="IVM62_001229"
     CDS             28037..28375
                     /locus_tag="IVM62_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP6845198.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            28770..29804
                     /locus_tag="IVM62_001230"
     CDS             28770..29804
                     /locus_tag="IVM62_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6845199.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29797..>30406
                     /locus_tag="IVM62_001231"
     CDS             29797..>30406
                     /locus_tag="IVM62_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845200.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVA"
BASE COUNT         9887 a         5540 c         6457 g         8522 t
ORIGIN      
        1 ttatattttt tctttttata aaatacactt attataattt tgttttttat tgacagtaaa
       61 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
      121 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      181 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      241 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      301 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      361 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      421 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      481 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      541 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      601 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      661 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      721 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      781 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      841 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      901 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      961 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
     1021 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1081 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1141 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1201 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1261 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1321 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1381 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1441 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1501 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1561 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1621 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1681 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1741 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1801 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1861 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1921 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1981 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     2041 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2101 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2161 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2221 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2281 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2341 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2401 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2461 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2521 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2581 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2641 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2701 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2761 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2821 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2881 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2941 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     3001 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3061 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3121 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3181 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3241 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3301 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3361 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3421 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3481 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3541 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3601 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3661 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3721 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3781 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3841 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3901 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3961 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     4021 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4081 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4141 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4201 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4261 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4321 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4381 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4441 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4501 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4561 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4621 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4681 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4741 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4801 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4861 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4921 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4981 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     5041 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5101 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5161 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5221 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5281 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5341 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5401 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5461 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5521 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5581 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5641 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5701 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5761 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5821 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5881 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5941 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     6001 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6061 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6121 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6181 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6241 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6301 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6361 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6421 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6481 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6541 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6601 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6661 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6721 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6781 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6841 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6901 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6961 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     7021 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7081 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7141 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7201 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7261 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7321 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7381 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7441 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7501 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7561 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7621 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7681 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7741 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7801 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7861 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7921 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7981 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     8041 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8101 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8161 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8221 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8281 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8341 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8401 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8461 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8521 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8581 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8641 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8701 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8761 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8821 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8881 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8941 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     9001 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9061 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9121 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9181 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9241 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9301 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9361 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9421 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9481 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9541 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9601 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9661 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9721 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9781 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9841 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9901 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     9961 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
    10021 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10081 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10141 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10201 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10261 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10321 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10381 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10441 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10501 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10561 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10621 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10681 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10741 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10801 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10861 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10921 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    10981 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    11041 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11101 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11161 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11221 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11281 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11341 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11401 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11461 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11521 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11581 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11641 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11701 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11761 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11821 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11881 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    11941 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    12001 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12061 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12121 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12181 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12241 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12301 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12361 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12421 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12481 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12541 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12601 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12661 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12721 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12781 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12841 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12901 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    12961 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    13021 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13081 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13141 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13201 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13261 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13321 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13381 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13441 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13501 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13561 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13621 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13681 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13741 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13801 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13861 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13921 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    13981 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    14041 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14101 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14161 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14221 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14281 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14341 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14401 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14461 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14521 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14581 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14641 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14701 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14761 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14821 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14881 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    14941 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    15001 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15061 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15121 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15181 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15241 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15301 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15361 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15421 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15481 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15541 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15601 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15661 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15721 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15781 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15841 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15901 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    15961 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    16021 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16081 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16141 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16201 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16261 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16321 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16381 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16441 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16501 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
    16561 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16621 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16681 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16741 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16801 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16861 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16921 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    16981 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    17041 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17101 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17161 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17221 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17281 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17341 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17401 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17461 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17521 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17581 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17641 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17701 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17761 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17821 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17881 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    17941 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    18001 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18061 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18121 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18181 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18241 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18301 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18361 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18421 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18481 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18541 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18601 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18661 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18721 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18781 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18841 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18901 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    18961 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    19021 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19081 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19141 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19201 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19261 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19321 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19381 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19441 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19501 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19561 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19621 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19681 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19741 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19801 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19861 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19921 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    19981 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    20041 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20101 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20161 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20221 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20281 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggattgcaa
    20341 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20401 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20461 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20521 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20581 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20641 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20701 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20761 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20821 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20881 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    20941 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    21001 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21061 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21121 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21181 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21241 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21301 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21361 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21421 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21481 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21541 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21601 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21661 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21721 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21781 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21841 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21901 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    21961 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    22021 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22081 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22141 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22201 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22261 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22321 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22381 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22441 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22501 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22561 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22621 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22681 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    22741 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    22801 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    22861 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    22921 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    22981 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    23041 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    23101 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    23161 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    23221 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    23281 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    23341 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    23401 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    23461 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    23521 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    23581 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    23641 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    23701 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    23761 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    23821 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    23881 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    23941 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    24001 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    24061 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    24121 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    24181 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    24241 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    24301 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    24361 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    24421 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    24481 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    24541 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    24601 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    24661 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    24721 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    24781 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    24841 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    24901 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    24961 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    25021 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    25081 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    25141 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    25201 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    25261 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    25321 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    25381 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    25441 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    25501 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    25561 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    25621 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    25681 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    25741 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    25801 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    25861 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    25921 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    25981 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    26041 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    26101 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    26161 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    26221 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    26281 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    26341 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    26401 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    26461 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    26521 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    26581 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    26641 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    26701 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    26761 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    26821 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    26881 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    26941 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    27001 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    27061 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    27121 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    27181 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    27241 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    27301 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    27361 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    27421 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    27481 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    27541 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    27601 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    27661 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    27721 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    27781 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    27841 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    27901 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    27961 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    28021 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    28081 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    28141 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    28201 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    28261 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    28321 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    28381 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    28441 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    28501 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    28561 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    28621 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    28681 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    28741 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    28801 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    28861 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    28921 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    28981 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    29041 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    29101 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    29161 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    29221 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    29281 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    29341 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    29401 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    29461 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    29521 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    29581 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    29641 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    29701 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    29761 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    29821 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    29881 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    29941 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    30001 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    30061 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    30121 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    30181 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    30241 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    30301 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    30361 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcga
//
LOCUS       DACCIQ010000027        29103 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCIQ010000027 DACCIQ010000000
VERSION     DACCIQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.027"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..1030)
                     /locus_tag="IVM62_001232"
     CDS             complement(<1..1030)
                     /locus_tag="IVM62_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6845201.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IVM62_001233"
     CDS             complement(1112..1801)
                     /locus_tag="IVM62_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6845202.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVM62_001234"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVM62_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP6845203.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IVM62_001235"
     CDS             complement(2328..2831)
                     /locus_tag="IVM62_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP6845204.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVM62_001236"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVM62_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP6845205.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVM62_001237"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVM62_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP6845206.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVM62_001238"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVM62_001238"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP6845207.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVM62_001239"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVM62_001239"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP6845208.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IVM62_001240"
     CDS             complement(6443..7462)
                     /locus_tag="IVM62_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6845209.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IVM62_001241"
     CDS             complement(7635..8522)
                     /locus_tag="IVM62_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6845210.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVM62_001242"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVM62_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP6845211.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVM62_001243"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVM62_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP6845212.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVM62_001244"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVM62_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6845213.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IVM62_001245"
     CDS             9753..10397
                     /locus_tag="IVM62_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6845214.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IVM62_001246"
     CDS             complement(10453..10956)
                     /locus_tag="IVM62_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6845215.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IVM62_001247"
     CDS             11553..12518
                     /locus_tag="IVM62_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6845216.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IVM62_001248"
     CDS             complement(12617..13789)
                     /locus_tag="IVM62_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845217.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IVM62_001249"
     CDS             complement(13776..14921)
                     /locus_tag="IVM62_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845218.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IVM62_001250"
     CDS             complement(14935..15867)
                     /locus_tag="IVM62_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845219.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IVM62_001251"
     CDS             15984..17063
                     /locus_tag="IVM62_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6845220.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IVM62_001252"
     CDS             17060..17689
                     /locus_tag="IVM62_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6845221.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IVM62_001253"
     CDS             complement(17799..18026)
                     /locus_tag="IVM62_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6845222.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IVM62_001254"
     CDS             complement(18129..20717)
                     /locus_tag="IVM62_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP6845223.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IVM62_001255"
     CDS             complement(20737..21396)
                     /locus_tag="IVM62_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6845224.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVM62_001256"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVM62_001256"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP6845225.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IVM62_001257"
     CDS             complement(22169..23035)
                     /locus_tag="IVM62_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6845226.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IVM62_001258"
     CDS             23144..23785
                     /locus_tag="IVM62_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP6845227.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVM62_001259"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVM62_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP6845228.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IVM62_001260"
     CDS             complement(25226..26125)
                     /locus_tag="IVM62_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6845229.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IVM62_001261"
     CDS             complement(26127..26924)
                     /locus_tag="IVM62_001261"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP6845230.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IVM62_001262"
     CDS             complement(26902..27606)
                     /locus_tag="IVM62_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP6845231.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IVM62_001263"
     CDS             27737..28312
                     /locus_tag="IVM62_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP6845232.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IVM62_001264"
     CDS             28430..>29103
                     /locus_tag="IVM62_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6845233.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6169 c         5232 g         9141 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACCIQ010000028        28600 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCIQ010000028 DACCIQ010000000
VERSION     DACCIQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.028"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            237..1877
                     /locus_tag="IVM62_001265"
     CDS             237..1877
                     /locus_tag="IVM62_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6845234.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IVM62_001266"
     CDS             2031..3353
                     /locus_tag="IVM62_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6845235.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IVM62_001267"
     CDS             complement(3415..3846)
                     /locus_tag="IVM62_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845236.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IVM62_001268"
     CDS             complement(3910..4746)
                     /locus_tag="IVM62_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP6845237.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IVM62_001269"
     CDS             complement(4936..5238)
                     /locus_tag="IVM62_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6845238.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVM62_001270"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVM62_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6845239.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IVM62_001271"
     CDS             complement(5692..5958)
                     /locus_tag="IVM62_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP6845240.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IVM62_001272"
     CDS             complement(6212..6937)
                     /locus_tag="IVM62_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6845241.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IVM62_001273"
     tRNA            complement(7303..7373)
                     /locus_tag="IVM62_001273"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IVM62_001274"
     CDS             7490..8242
                     /locus_tag="IVM62_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP6845242.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IVM62_001275"
     CDS             8258..8737
                     /locus_tag="IVM62_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP6845243.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IVM62_001276"
     CDS             complement(8988..10568)
                     /locus_tag="IVM62_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP6845244.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IVM62_001277"
     CDS             complement(10561..11304)
                     /locus_tag="IVM62_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845245.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IVM62_001278"
     CDS             complement(11480..12256)
                     /locus_tag="IVM62_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6845246.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IVM62_001279"
     CDS             complement(12504..13340)
                     /locus_tag="IVM62_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP6845247.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IVM62_001280"
     CDS             complement(13351..14280)
                     /locus_tag="IVM62_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP6845248.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IVM62_001281"
     CDS             14472..14825
                     /locus_tag="IVM62_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6845249.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IVM62_001282"
     CDS             complement(14899..15555)
                     /locus_tag="IVM62_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6845250.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IVM62_001283"
     CDS             15781..17715
                     /locus_tag="IVM62_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6845251.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IVM62_001284"
     CDS             complement(17801..19024)
                     /locus_tag="IVM62_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP6845252.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IVM62_001285"
     CDS             complement(19018..20916)
                     /locus_tag="IVM62_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP6845253.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IVM62_001286"
     CDS             complement(20910..22028)
                     /locus_tag="IVM62_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6845254.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IVM62_001287"
     CDS             complement(22111..22944)
                     /locus_tag="IVM62_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6845255.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IVM62_001288"
     CDS             complement(22937..23854)
                     /locus_tag="IVM62_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6845256.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23877..24056)
                     /locus_tag="IVM62_001289"
     CDS             complement(23877..24056)
                     /locus_tag="IVM62_001289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845257.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24081..25205)
                     /locus_tag="IVM62_001290"
     CDS             complement(24081..25205)
                     /locus_tag="IVM62_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6845258.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="IVM62_001291"
     CDS             25388..26296
                     /locus_tag="IVM62_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845259.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="IVM62_001292"
     CDS             26437..28050
                     /locus_tag="IVM62_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP6845260.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8890 a         5621 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta
//
LOCUS       DACCIQ010000029        27529 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCIQ010000029 DACCIQ010000000
VERSION     DACCIQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.029"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            143..1210
                     /gene="rlmN"
                     /locus_tag="IVM62_001293"
     CDS             143..1210
                     /gene="rlmN"
                     /locus_tag="IVM62_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6845261.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1403..2506)
                     /locus_tag="IVM62_001294"
     CDS             complement(1403..2506)
                     /locus_tag="IVM62_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6845262.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3059..3607
                     /gene="aac(6')"
                     /locus_tag="IVM62_001295"
     CDS             3059..3607
                     /gene="aac(6')"
                     /locus_tag="IVM62_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6845263.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            4153..5814
                     /locus_tag="IVM62_001296"
     CDS             4153..5814
                     /locus_tag="IVM62_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6845264.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(5884..6231)
                     /locus_tag="IVM62_001297"
     CDS             complement(5884..6231)
                     /locus_tag="IVM62_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP6845265.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            6315..7256
                     /locus_tag="IVM62_001298"
     CDS             6315..7256
                     /locus_tag="IVM62_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845266.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            7257..8300
                     /locus_tag="IVM62_001299"
     CDS             7257..8300
                     /locus_tag="IVM62_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845267.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            8306..9376
                     /locus_tag="IVM62_001300"
     CDS             8306..9376
                     /locus_tag="IVM62_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845268.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            9366..10319
                     /locus_tag="IVM62_001301"
     CDS             9366..10319
                     /locus_tag="IVM62_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6845269.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            10331..11341
                     /locus_tag="IVM62_001302"
     CDS             10331..11341
                     /locus_tag="IVM62_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6845270.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(11626..12447)
                     /locus_tag="IVM62_001303"
     CDS             complement(11626..12447)
                     /locus_tag="IVM62_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6845271.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(12544..12822)
                     /locus_tag="IVM62_001304"
     CDS             complement(12544..12822)
                     /locus_tag="IVM62_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845272.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            13090..15099
                     /gene="metG"
                     /locus_tag="IVM62_001305"
     CDS             13090..15099
                     /gene="metG"
                     /locus_tag="IVM62_001305"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP6845273.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(15578..16165)
                     /locus_tag="IVM62_001306"
     CDS             complement(15578..16165)
                     /locus_tag="IVM62_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845274.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            16340..17662
                     /locus_tag="IVM62_001307"
     CDS             16340..17662
                     /locus_tag="IVM62_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845275.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            17817..18593
                     /locus_tag="IVM62_001308"
     CDS             17817..18593
                     /locus_tag="IVM62_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6845276.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            18586..19155
                     /gene="rnmV"
                     /locus_tag="IVM62_001309"
     CDS             18586..19155
                     /gene="rnmV"
                     /locus_tag="IVM62_001309"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP6845277.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            19148..20032
                     /gene="rsmA"
                     /locus_tag="IVM62_001310"
     CDS             19148..20032
                     /gene="rsmA"
                     /locus_tag="IVM62_001310"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP6845278.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            20483..21112
                     /locus_tag="IVM62_001311"
     CDS             20483..21112
                     /locus_tag="IVM62_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845279.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(21236..22345)
                     /gene="ugpC"
                     /locus_tag="IVM62_001312"
     CDS             complement(21236..22345)
                     /gene="ugpC"
                     /locus_tag="IVM62_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP6845280.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            22554..22976
                     /gene="mgsA"
                     /locus_tag="IVM62_001313"
     CDS             22554..22976
                     /gene="mgsA"
                     /locus_tag="IVM62_001313"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP6845281.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            23299..23835
                     /locus_tag="IVM62_001314"
     CDS             23299..23835
                     /locus_tag="IVM62_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6845282.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(23875..25638)
                     /locus_tag="IVM62_001315"
     CDS             complement(23875..25638)
                     /locus_tag="IVM62_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845283.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(25641..27356)
                     /locus_tag="IVM62_001316"
     CDS             complement(25641..27356)
                     /locus_tag="IVM62_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845284.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         8895 a         4974 c         5577 g         8083 t
ORIGIN      
        1 cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc
       61 gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt
      121 tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga
      181 attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga
      241 atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt
      301 gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga
      361 agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac
      421 tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa
      481 tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg
      541 agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga
      601 acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt
      661 aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat
      721 tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca
      781 agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg
      841 gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa
      901 aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga
      961 gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt
     1021 gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt
     1081 attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca
     1141 tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt
     1201 tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa
     1261 ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt
     1321 ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct
     1381 tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg
     1441 tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat
     1501 aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg
     1561 tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca
     1621 catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat
     1681 aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat
     1741 agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg
     1801 tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac
     1861 tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt
     1921 ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga
     1981 cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa
     2041 taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca
     2101 ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa
     2161 tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc
     2221 aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga
     2281 tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct
     2341 ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc
     2401 ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg
     2461 catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc atttccttct
     2521 ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg
     2581 gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa
     2641 tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa
     2701 tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat
     2761 tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt
     2821 ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga
     2881 tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt tttattttat
     2941 aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga
     3001 gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat
     3061 gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact
     3121 gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat
     3181 gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg
     3241 tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc
     3301 ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc
     3361 cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag
     3421 tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga
     3481 acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc
     3541 aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc
     3601 tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta
     3661 cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg
     3721 gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt
     3781 tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga aactggcttt
     3841 tgaacaccgt ttattcagat aagggactgt gacaagactt ttgtcacagt ttctttttgt
     3901 ttattctttt ttatctttgt atggcaataa tagacaatat aacgaaaatt atttaatggc
     3961 ttttggaaga ccatggaaaa ccttgtacaa agctattttt tctgaaaaat tccttgaaac
     4021 ctcacgaaaa aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat
     4081 tattcattaa actcttaaac aacttcagaa aatattaatt attctgaaaa ttccaataaa
     4141 ggggtacgta aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg cggccttgta
     4201 cttgctggat gttatggcgg cagttcagcc acaagcagta gttcagcaaa aagcggttca
     4261 gcagatggag gaggcgtatt taacttagtc gtgcctcaag aaatgcctac agctgactta
     4321 tcagttgcaa cagatacaat cagttttaca gcattgaata acgtttatga aggaatttac
     4381 cgtcttgatg aagacagcaa accacagccg gcaggagcta gcgagttggc agaagtcagt
     4441 gaagacggat taacttacaa aatcaaatta cgagaagacg caaaatggtc aaatggtgat
     4501 cctgttacag cagcggatta cgtttatggc tggcaacgaa cagtagaccc agctacagct
     4561 tctgaatatg cttacctgta tgcgccagtt gaaaatgcag aagacattac agctggaaag
     4621 aaagataaat cagaactagg gatcaaagca gttggtgact atgaattaga aatcaaattg
     4681 acgaaacaaa caccatattt ccaatatctg cttgctttcc catcattttt cccacaaaaa
     4741 caatccgtag tggaagaaaa tggggatgcc tatgcatctg caagtgataa agctgtttat
     4801 aatggaccat tcacattaga agattttgat ggaccaggaa cagatacaga atggacttat
     4861 aagaaaaatg atcattactg ggacaaagat acagtaaaac tttctgaaat caaagtcagt
     4921 gtagtaaaag aatcttctac tgcattgaac ttattcaaag atggccaagc agatgacgtg
     4981 atcttgtctg gtgagttagc acagcaaaac gcaaacgatc cagcttatac ttctgttaaa
     5041 gaagcgcgga caagctatat cgaattcaat caacgtgaaa agaattcacc attcaacaat
     5101 gtgaacctaa gaaaagctat ttcttattct atcgatcgtg aagctttagt aaaacaagta
     5161 ctaggtgacg gatcagttgt ctctacagga ttgatcccat cagatatggt aaaaagtcca
     5221 acaacgaacg aagactttgc aaaagaagct ggaaaattag tttcttatga caaagataaa
     5281 gcaaaagaat attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt agaatttgat
     5341 ttgatggctt ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc
     5401 caagaaaact tggatggcgt aacagtcaaa ccaacacctg ttccattctc tgtacgtctt
     5461 gaccgctcaa cttcgggtga ttttgacgca gtactaggcg gatgggctgc tgactatgca
     5521 gacccaagca gctttactga tttgtttgtc acaggtaatt catacaaccg tggtcgctgg
     5581 agcaatgcag aatatgacga agcagttaaa gcagcttcat caacagacgc tggcgatgaa
     5641 caagcccgtt gggatgattt ccaaaaagca atcaagatca tctctgatga tatgggtgtc
     5701 gttccggtgt accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt
     5761 caccatgcgg caggcgcttc atgggattac aaatggacat acgttgaaga ataatcgtta
     5821 ataccgtgag catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt
     5881 cacttatcct aacaaagaaa aaagcatgaa aatagcagaa agagcaatta gtattccggc
     5941 aatgatcagc caaaagctgt acataccgta gctggcagac gagagcgagg aaaattctgg
     6001 ttttttcttt cggttccttg ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa
     6061 acccgaagaa aagacccata aaaaaccgcc gataatcaga aacggcaatg accataagaa
     6121 tagatcatta gataattgaa tgagtgtaaa ggaatggttg atgaggtttt ttattaaaat
     6181 aaccagaatc agtagaaaag ctactgcgta ggggatagat ttttttttca tttgattgct
     6241 cctttatttt tggttcatta tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct
     6301 agggggaaac attagtgaat agttatatca aatatgtatt aaaacgtgtc tttttcatga
     6361 tcatcacgct ttggttaatt gcaacaatca cctttttctt gatgcaattg ctgccgggca
     6421 cgccatatac gaatcaggaa cgtttgagtc ctgaaacaat cgagatgcta aataaacaag
     6481 tcggtttgga taaaccagtt atcgtacaat atgggattta tctttctaat ttgcttcaag
     6541 gagattttgg aatttccttc caatttaaaa accagccagt cgcacatttg ttagctggaa
     6601 gaatcggacc atccttgcag ttagggttac aggcaattat ctttggaaca gtcctaggga
     6661 cgattttagg aacgatctct gccatgaagc aaaatacatg ggcagataca tcttctacac
     6721 tcgtagcgat attaggtcgt tccattccaa actttgtatt tgctgtttta ttacaatata
     6781 tctttgcaat caaattacaa gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa
     6841 tcttaccaac aattgcgttg gccatgtcgc cgcttgctga ctctgctcgg tttatccgta
     6901 cggaaatggt ggaagtactg catagtgatt atgtggaatt agcaaaagca aaaggattga
     6961 gccgttggga aatcgccttt aaacatggtc ttcgaaatag tttgattcca ttgatgactt
     7021 tattaggacc gttagctgta gcattgatga ccggatcact ggtggtagaa aatatctttg
     7081 ctattcctgg tatcggggaa caattcgtta agtcaatcac aacaaatgat tatcctacga
     7141 tcatggctgt aacgatcctt tactcattta tgctgatttt tgttattttg atcgtggatc
     7201 tattgtacgg tctagttgat ccaagaattc gtgtttcaga agggagccga ggataaatgg
     7261 aaatgatacc aaagcgttat gaaacagttg cagatatccc tgcggatgaa tttcttccat
     7321 tacaaaaaaa taccgaagaa gaacgtgaac agattactgc accttctttg aattttatcc
     7381 aagattcatg gcgccgttta aagaaaaaca aagctgccgt tatttcaatg gttttactat
     7441 tggtcatcat tttgatctct gtgatcacaa tctttgtttc tcctcatgat ccaactgccc
     7501 aaaacgtggc ttatatcaat ttgcctcctc gtattccggg aatcaatatc gatggattga
     7561 atggaaaagc aatggttggt ggtgaacttg ttgataaata tgctcaagca aacgtccctt
     7621 caaacgtaaa tttcttttta ggaacagacg gtttgggacg tgatgtatta agccgcttat
     7681 ttatgggaat gagaatctcg ttgctgatcg catttattgc tgcattacta gatgtaacga
     7741 ttggtgtagc ttacgggttg gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc
     7801 aacgtttttt ggaaatctta tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg
     7861 tcgtttttga accaggaatt ttttcaatcg tggctgctat ggccatcaca aactggatac
     7921 caatggcacg tatcgttcgt gcacaaacaa tgaaattaaa agatcaagaa tatgttttag
     7981 ctggattgac attaggtgaa tcaagaatga aaattgcatt caaacatatt ttgccgaata
     8041 tctctagtgt cattattatt caaatgatgt ttagtattcc atctgctatt ttcttcgaag
     8101 cctttttaag ctttatcggt ttaggtctta cgcctccgtc agcttcatta ggaacgatgt
     8161 tgagcgaagg gtacaagaca ttcctgtatc tgccttatct attatggatt ccggcagtaa
     8221 ctttatcggt tatcatgatt ggctttaact tacttgctga tggattgcgt gatgcctttg
     8281 atcctaaaat gaaagagtga agacgatgac gaaaatttta gaagtgaaag acttagaaat
     8341 ctcttttgat acgtatgctg gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa
     8401 taaaggagaa acattggcga tcgttgggga atctggaagt ggaaaatcag tgacgaccag
     8461 aagcatcatg cgcttgctgt caagcaacgc caatatagat gctggtcaaa tcctctttaa
     8521 aggtaacgat atcgttggaa aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat
     8581 agcgatgatt ttccaagatc caatgacctc tttagatccg acgatgccca ttgggaagca
     8641 agtcgctgag tcccttagaa agcataataa agtttcaaaa aaagagggac aaaaagctgc
     8701 attagaattg cttaaattag ttggaatacc cgaagctgag aaacgtatca acagttatcc
     8761 gcatcagttc tcaggaggtc agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta
     8821 tccggaaatc ctgattgctg atgaacctac aacagcgctt gatgtgacta ttcaagcaca
     8881 gattttggag ctgctgaaag aaattcagac aaaaatcgat acatcaatta tctttatcac
     8941 tcatgacctt ggtgtagtag cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag
     9001 aatcgttgaa gttggaacat cggaagaaat tttctacaac ccgcaacatc cctatacttg
     9061 gggactgctt ggttcaatgc cgacattaga tagtgcgaac gatcgattgt atgctattcc
     9121 tggttctcca ccggatttat tgaacccgcc aaaaggagac gcattctatc caagaaatga
     9181 attcgcgatg aaaatcgatg cagaacaaga accaccattt tttgaactat caaaaacaca
     9241 caaggcagca acttggctac ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat
     9301 ccaacgtcgc tgggctatct ttaaagagaa acaaaaacat acttacggtc aaggaggaat
     9361 tgctgatgcc aaataaacta cttgaagtta aaggattgaa acagtatttc aatgtaggaa
     9421 aaaagaatga agtacatgct gtaaatgaca tcagctttca catttatgaa ggagaaacat
     9481 tcggtttagt tggagaatct ggaagcggaa aatccactac tggccgtacg atcattcgct
     9541 tgaatgaacc tactgatggt gagatattat tcgacggtca agatataacg aagatcaaag
     9601 ataaaaaagg tttgacgaaa ttccggcatg atgttcagat gatctttcaa gatccatacg
     9661 cttcgttgaa tcctcgtatg aaagtcagag atatcattgc cgaaggaatt gatgttaatg
     9721 gactagctaa atcatctgaa gagcgtgcaa aaaaagtcga tgatctattg agaactgttg
     9781 gattgaatcc aagccacggt actcgttatc ctcatgaatt ttcaggtggg caacgccagc
     9841 gtattggtat tgcacgagca ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa
     9901 tttctgcact agatgtgtcg atccaagccc aagtggtcaa tttgttgcaa gatctccaaa
     9961 aagaacatca attgacctat ctatttattg cacatgattt gtcgatggtt aaacatatta
    10021 gtgaccgaat cggtgtaatg cataacggct tgttattaga gatgggaaca agtgatgaaa
    10081 tctataatca cggtgttcat ccttatacag aaagtttgtt atctgctatt cctctaccag
    10141 atccagacca tgagagaaaa cgcagaagaa tcaaatatca acctgaacca gatgacggac
    10201 aagtacgtca acttcgtgaa atcgcaccag aacattttgt ctatgcaact gagcaggaag
    10261 tcccatatta cgcaaaaaaa ctaaaacgac aaaaggaatc tcttctggtt gctaactagg
    10321 agggacatag atgctaaaag cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca
    10381 gcaattgatt cgtacttttg gatgtgctcg atttacctat aaccatttgc tgaaaaagcg
    10441 gcagaaaagt tggcaacaaa caggagtagc caatttttcg ctcacacccg ctacattaaa
    10501 aaaagaatac ccattcttaa aagaagtaga cagtcttgct ttagcaaatg cacagcttaa
    10561 tttggatcgg gcttttcgca attatttcaa gggacgtgcc agcttcccaa agcttaaaac
    10621 gaaaaaaagt atgtggcaat cttatacgac caacaaccaa acccgtacga tctatctgga
    10681 aaatggctac ttaaagttac caaaacaaaa agaactaatc aagataaatc agcatcggcc
    10741 agttgaagga tcgattcgct ccgctacgat ttctgctcga tataatgaag aattttatgt
    10801 cgcactactt tgtgatgttt cgccagtaaa aaaagaatct ttagctaaat ggatcgggat
    10861 cgcctatcat ccgaagacat tgatccaaac atctcgacct ctagaagtaa ctttaccaaa
    10921 atttcaccag acagaagaaa agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg
    10981 ctccgctcac catagaaaga tcaggttaga tcaggcaagc aattatcaga aacaaaagag
    11041 aaaagtaatg gatctttatc tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt
    11101 atctgggaaa ttggtcaaac aatatgacta cttatttgta gaatcttttc caaaagaaga
    11161 ggcacatgct gatttttcta ttcacgactg gcataaatta ataacaaaat tgcgttataa
    11221 gtcccaatgg tataataaaa aatttctttt gatcaatacc gacggagcag aagaaagcaa
    11281 ttccgtaaga aagagccaag tagtagaaaa aatgggcaga cattcggtta tcaagggata
    11341 aaaaatgaat gaaactgata ggagcgttag ggttagcctt gttaataaaa agtcttaatg
    11401 acttttgttt ctaggaattt ttcttttgaa gaaaagctca ctaattatga caaaccaata
    11461 aaacaatagc agaaaaagtt cgtacgagct ttttctgcta ttgttaattt tttattcaga
    11521 aagtttacga atacatcaaa attctgaaac aaaaaacgta actcacaaag agttgttttt
    11581 gcgttttttc ttgaatgatt aggtctgaat cttttgctca caaccttatt catggtgccc
    11641 cggcttgagc tgatagattt ttgaaggacg gccgattcct acaggacgtt caccgatctc
    11701 atcgaaatat tggagcatag ctttcttaaa attggaatga tcgatcgttt tataatcgat
    11761 ccctaagaat ttagcaaata cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac
    11821 ttgtagaact tgcggctcgt gttccatttt attgactaca cggttgaatg ctttcaagat
    11881 gatttgacta tggtcaaaag ccaaagtatc tttgccagaa gactctccag tgactaaatc
    11941 aagcgagata gccacttctc cgctagataa atggatcttg ttactatgac gttctagtgt
    12001 gaaccagcgt acttccttgg catcatctcc ggcgattagc ggttcttctc caataaaggc
    12061 taagtagctg actgtgacga cccagcctcg cggatctcga ttaggcgtac tgaatgtatg
    12121 cagttgctca atattttctt ttgaaataac cacaccagtt tcttcctttg tttctcttaa
    12181 gacgctttct ccagttgact catccttttg gacaaagcca ccaggcaatg cccatgaatt
    12241 tcgataagga tgcccttttc tttggatcaa caaaactttc agctgatctt cttctttgtt
    12301 atagcacatc agtacgatat cgacagtcaa agagggcttg tcatattctg gacgttcctg
    12361 tttttgatac cagttaagaa attcttcttc acttgcttca tgttcataat ataatttttc
    12421 ttcttcttta gaagcaaaac ggttcatctg accacctcca ttgacagtaa aagtactttt
    12481 taccttattg tagcagactc tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat
    12541 catttaaata tgaaaaataa atggaagtat gagattgaat aaaaagaaaa gcagataaaa
    12601 cggcaatact ttataccgct cttcacgaat ggcaaagaag ccacaaataa cgatatatac
    12661 gtcaatgata aaacgcaaaa tccaagctgc tgtcataaaa ctgttcaaat gcccatggag
    12721 gttaaaataa ccatacatac gaatataaaa atagctgaat attaaatgtg ctaatgaaag
    12781 aaaagcaata ctaagtgtaa aaaaaagatg tttcttctgc atgattgttc cagtccctta
    12841 ctaagataga tagtctttat tataccacgt gtgaggggag aaataaaaag ccgtttttta
    12901 gtagatatga aacaaaacaa aaacccgcat ttattcaacc tattcatcct tttaaaggat
    12961 agattgaacg tagatagaag tcgtttatcc agtctttttc attttgaaaa aaagctaaca
    13021 gcatgctttt tttatggtat actagcaagg aaacgaattc gcatcatgcg ataaaggagg
    13081 aagagccccg tggcagaaaa ggaaactttt tatatcacta cgccaatcta ttatccaagc
    13141 ggcaaacttc atatcggcaa ctcgtatact acgattgcat gtgacgcaat tgctcgttat
    13201 aaacggttga tgggctttga tgtgttttat ctcactggag tggatgaaca tggtcaaaaa
    13261 atcgaaacaa aagcagaaga actaggtgta aaaccgcaag aatatgtaga taaaatggcg
    13321 gctgatgtca agaaattatg gaaaacgctt gatatcagtt atgataaatt cattcgcacg
    13381 acagatgatt accataaagc agctgttcaa aaaatatttg atcgtttatt agaacaggga
    13441 gatatttact taggtgaata tgaaggctgg tactctgttt ctgacgaaga attctttaca
    13501 gaaacacaac ttgcagaagt gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg
    13561 ccaagtggac acgaagtaga attggtaaaa gaagaatcat acttcttccg catgagcaaa
    13621 tacgcagacc gtctattgga atactatgaa gaacatcccg aattcatcca gccagaatct
    13681 cgtaaaaacg aaatgatcaa caactttatc aaaccaggat tagaggatct tgcagtttca
    13741 cgaacaactt ttacttgggg agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta
    13801 tggatcgatg cattatccaa ttatattact gcactaggat atggctccga agatgaaagt
    13861 ctattcgaaa aatactggcc tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt
    13921 catacgatct attggccaat catgctgatg gcccttgatt tgcctctacc taaaaaagta
    13981 tttgggcatg gctggctatt gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc
    14041 gtttatcctg aaatgcttgt ggaacgttat ggtttagatg cactccgtta ttatttgctt
    14101 cgtgctgttc cgtttgggtc agatggtgtc tttacgccag aagattttgt ttcccgtgtc
    14161 aattatgatc tggcaaatga tctagggaac ttattgaatc gaacgattgc tatgatcaac
    14221 aaatattgtg atggactcgt tcctgactat gcctcacttg tcactccatt tgatagtgaa
    14281 ctgtctacga cggctgctaa cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa
    14341 ttcaatacag cattggctga aatctgggtg ctgatttctc gtgcaaacaa atacattgat
    14401 gaaacagaac catgggtatt agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc
    14461 atgatccatt tagctgaatc tttgcgtatc gcagcgatat tacttcagcc aatcatgaca
    14521 gaaacgccag agaagatctt caatcaacta ggattggact ctgaaacaat gaatcttgaa
    14581 ggattacact ttggcgaatt tccttcagga acaaaagtag ttgcaaaagg tacaccaatc
    14641 ttcccacgtt tagatatgga agaagaagtt gcttttattc aagaaaaaat gtctgaaggt
    14701 actcaaacaa acgaagatac agtcaaatgg gatccagaag aaacagaact tgtttcaacg
    14761 aaagaaaaac aaatcaaatt tgatgtattt gaaaaagtag aactaaaagt agcagaagtc
    14821 atcaactgtc aaaaagtaga aggggcagat aagttgctgc aattccgact agatgctgga
    14881 gacagccaag accgtcaaat cttgtcagga atcgctgaat tttatcctga tccaagcgaa
    14941 ttgatcggga aaaaagtcgt cattgtagca aatctgaaac cacgaaagat gcgtggacaa
    15001 atcagtcaag gaatgatcct ttctgcagaa gcaccagatg gctctttaca agtaatcgaa
    15061 gcaccaaaat ccatgccaaa tggctcagaa atcgcataga aattcaaagg gggtatgaca
    15121 ctagttgtgt cataccccct ttttccgaaa tttgagaagc aattttcgta aattccttct
    15181 tattacttga agccaatcat ataatacaga aacagagaac tatccaaaat aaatcctaga
    15241 aaaagaagta cgagcttcgc tgatttttgt taatttagaa taatactaat aggaatcgta
    15301 aaaacagaaa aaaagcataa tgataaaggg tcgggcagat tttaattgat tcatctggta
    15361 ctctttttta ttgtccttac attcataaag tttgaatgca atgactttat tacatctata
    15421 tttatatcta acagtagcgt tgatgtgtta attataagtt ctatcacttt atgttcaatt
    15481 agtttgtgct cttatatgtg tgattgaaaa tatagataaa aaaacaatca tcggctttga
    15541 cttcgatgat tgttttcttt attttgtaaa aatcctatca cttgatttta tggtaacctt
    15601 ttatttttgt cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa
    15661 agacatacca gaaaatcgta cgtccgatac cataatgttg atagagcttt gtcaacatca
    15721 cgtgtaacaa gatcaaccct aaaagcaagg agccagctat aaaccaatga ttatcccaat
    15781 gcatgacttc actaagtgcc atcactccaa aaggatagaa ataaaaatag atcccgacta
    15841 aggtcatccg ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa
    15901 gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga atacaacatc ccccacaaga
    15961 tcatcacagg acctttgatc aatgcaacga ttgcgatgaa caaagtcagc aatattagtt
    16021 tttgtgataa cgttgcaaca gtcattaatc caaataaaat taatgtgaac caatgccaat
    16081 gggactgctg ttgttcttta cgggaaaaat cgacccggtt ggataaataa tctgctaatt
    16141 tcggataaga acgcttaaaa tccataaact gttcctcctt cgtggaaaag gtaggtttag
    16201 tatacgaaaa ataaaaaaga aatacatccg tcttaagagg gagtttttca ctgaaagtgg
    16261 agaaaaaaga atggtaaatt aaaatattct cagttatact gaaaattata ccaaatttca
    16321 aagaagaaag aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc
    16381 attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt
    16441 tactgctagt actcagtcta caagtttgac attgctagct tggcttttgg gtggtttctt
    16501 aacgatctgt gctggattga cagtggctga attagctaca gcaatcccgg aaactggtgg
    16561 agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc agctttttat taggatgggc
    16621 gcaaagcttg atttactttc ctgctaatat tgctgcatta tcgattatct ttgcgacaca
    16681 attgacgaac ttattacaat tatctaccga ttatcttttg ttgattgcag tgatcaccgc
    16741 tgtttctgtg acagggttga atttgttagg gacaaaagtc ggtgcttctg tacaatcagt
    16801 gacgttgatt gttaaattaa taccaattgc tgtgattgtc atatggggat tattgacccc
    16861 aggacaagga actgtccagt tatttccaat cgaagctgga aaagatgtta cttttgtcga
    16921 aggattgagc agtgcgttgt tagcaacgtt gtttgcttat gacggctggt taggtgtcgg
    16981 tgcaatggca ggggaaatga aacggccgga aaaagatttg cctaaagcaa ttattcttgg
    17041 tttgagtttt gtaacagtcg tttatctatt gatcaatttt gtttttttga aaactttgcc
    17101 gattgatcat cttgccggta atttgaatgc agcttctgaa gcatcagacg tcatttttgg
    17161 aggaatcggt ggaaagctgg tcactattgg gatcttgatc tcggtatacg gtgcattgaa
    17221 cggttataca ctaactggga tccgcgtgcc ttatgcgatg gctttagaag acgagctacc
    17281 tttcagtaaa cagctgacaa agctttcaaa gaagtttacg gttccttatg ttcctgcggt
    17341 tttccagtta gcagtagctt gtattatgat gagccttggt tctttcgact ttttaacaga
    17401 catgttgatt tttgtgatgt ggctattcag tttactgatt tttatcggtg tctttatttt
    17461 aagaaaaaaa gcgccggaat tgccacgtcc atataaagta ccactttatc caatcgttcc
    17521 gattattgcg attctcggtg ctattttcat tttaggtatg acgatgatga cgcaaacgaa
    17581 attagcattg atcggtatag gtgttacctt gataggtatc ccagtctatt atcaaaaaaa
    17641 gaaaaaacgc tcagaagagt aaaatatttc aacaacagac agaaaaagta cgaactaggt
    17701 cctctagatc ttcgtgcttt ttctatttca ataaaacaag ttcttctatt ttgatttttg
    17761 ctacttctgc aagtagtgct tatgtgctaa agtaagaatg aaaaggagag ataaacatga
    17821 tatttgattc tcatacccat ttaaatgctg aacagttcaa tgaagatatt ccagaaacga
    17881 ttcagcgagc acaagaaatg ggggtaacga aaatggcggt cgtgggcttc gatacgccaa
    17941 cgattgaaaa gtctttgcaa ttaagccatg actatccaaa tatatacagt attatcggct
    18001 ggcatccgac agaggctggc agttatacga aagaaatcga aaataaatta caagaacaat
    18061 tgactttacc aaaggttgta gcattagggg aaattggctt ggattatcat tggatggaag
    18121 acccgaaaga agtccaagat aaagtatttc gcagacagat tgctatcgca aaagaaatga
    18181 acctaccaat cagtattcat actagagaag cacttgaaga cacgtatcaa atcctaaaag
    18241 aagaagatgt tcgagatatc ggtggaatca tgcatagttt cagtggtgat tatgaatggg
    18301 caaaacgatt tcttgactta gggatgcata tttcatttag cggtgtcgtc actttcaaaa
    18361 aagcattaga cgtccaagag gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa
    18421 cagatgcccc ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata
    18481 ctcgttatac agtagaaaag attgctgaac ttcggaatct tccgacagaa gaagtggctg
    18541 cccaaacatg gacaaatgcg caccgattat ttggtctaag cgaacatgga taagatgaag
    18601 atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt
    18661 gatgcagata cgatagaaac aatagggtct gcaatcaatg aagagatcct gatgcaaatt
    18721 gaacatgccc aagagactcg tggagtgatc gtttttaccg atcctgattt ttcaggagaa
    18781 aaaataagaa aaatcattat ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc
    18841 acacaagcta aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag tgatgctgct
    18901 attttagaag cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa
    18961 atcactagac aagacctttt agactatggt ttaatagctg gggcacaagc aaaagaaaaa
    19021 cgagaaaaac taggtgacga gttgcgcatc ggttacacga acggcaaaca attggaaaaa
    19081 cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa aatagtggag
    19141 gaacaacatg agtgaatatc gtgaaattgc gacaccctct agaacaaaag agattttatt
    19201 gaaacatggt ttttcattta aaaaaagttt aggacaaaat tttctgactg aaccaaatat
    19261 tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg
    19321 accaggaatc ggtgcattga cggaacaatt agccaagcat gcaaaacaag tcgtagcatt
    19381 tgagatcgat gatcgtttga tccctgtatt ggctgatacg atgcaaccct atgacaatgt
    19441 gactattgta catcaagata ttttaaagac cgatttgtca acagctgtta gagaaacatt
    19501 tcatgaggag cttccgttaa aagtcgtagc gaatttacct tactatatta cgacaccgat
    19561 catgatgcat tttcttgaat cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa
    19621 agaagtagct gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt cattatcgat
    19681 tgctgttcaa tattatatgg aagccagttt agcattcatc gtaccaaaaa cagtatttgt
    19741 ccctcagcca aatgtagatt cagcgatttt gaaactgaca agaagagata taccggcagt
    19801 cgaagtgaca gacgaaaaag catttttccg attgacaaaa gcggcatttc agcaaagaag
    19861 aaaaacgctt tggaataact tgcagcattc gtatggcaaa gatgaccaga caaaagcatg
    19921 gttagcaaaa agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg agactttgtc
    19981 attacaagaa ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag
    20041 gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
    20101 aatagttcct catgtttttc gtttgtttgg gcttattacc gcagatgcaa gcttttgaac
    20161 accgtttaag aaagaggttg tgggcctgcc gtttaactct aagtcacaag gaacaatttc
    20221 aaaaaacagc ttctcatgtt tttgagttag ttggtcttat tacttgatgc aagcttttga
    20281 ccactattat gccgaacaag gagatgtgac acaaattttg tcacatctcc tatttttgtt
    20341 tcttgatcaa aaaattctta tacttgatac tcacactcat tttgtatgga tttagaaaag
    20401 aatatgtgtc ttgacagtta ttagtaagtg agttatacta agtcgacatt tatgtgtcaa
    20461 gacatattag gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc
    20521 tttattgatt gggatggggg tcattatccc aatggtcatg cctaaaatca tgattccacc
    20581 agcttccttt actttggcgt ctcatgtgcc attatttatt gcgatgtttt ttacaccagg
    20641 agttgcagtg gctgttgcgc taggtacgac atttggtttc tttttaacaa cgccggttat
    20701 catcgcattg cgagcattgt cacatcttgt gtttgcgttg attggggctt ggtacttgca
    20761 aaagcatccg agtatcgttt taaaaaatgg acagtttact tttgctaatt ggcgtttcca
    20821 agggttcaat tttgtgatcg gtgtgattca ttctttagca gaaatgctag tagtcagcat
    20881 cttttacttt attggtaata tgcctgaaac atattattct caaggatatt tttatacagt
    20941 attcatcttg atggggattg gtgggcttat ccacagttta gttgattata atattgctta
    21001 ttttatagca ggaacactca gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa
    21061 agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa acgacatatt agacatgatc
    21121 tctcaaaaaa aaaactctca tctagtaatg gataataaat cctctaagat gagagttttt
    21181 tgtcctaaaa agagctgcac caacatcttc tggcacagct ctaacagggg gcaatttatg
    21241 gaagtttgat gacttgttct gtttctttat caaagaagtg gcctttgctg atgttgaagg
    21301 caaggtcgac tatttcgcct ggtttatgga aatctcttgc atctactttt gagatgaact
    21361 cagtagaacc agtttttgtg taaagcatcg tttcagctcc aagtaattcg gaaacgacaa
    21421 cttctgattt cactacggct tcaggtgaag cttcaagggc tacttgttca ctgtggatgt
    21481 cttctggacg aataccgaag ataacttctt ttccttcata acctttttca actaatactt
    21541 tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc atctgtgata acaccatttt
    21601 tcaatgtcac attaaagaag ttcatggcag gagatccaat aaagccggca acgaacatat
    21661 tgacgggtgt atcataaact tctttcggtg taccgatttg ttggataaat ccgtctttca
    21721 tgataacgat tcggtcagcc atagtcattg cttctgtctg atcgtgagta acatagatgg
    21781 tcgttgtttc aagacgctgg tgaagtttgg caatctcagc acgcatggct acacgtaatt
    21841 tagcatctaa atttgataat ggctcatcca ttaagaagac ttttgcatcc cgaacgattg
    21901 cccgtcccaa tgccacacgt tggcgttgtc caccagataa agcagctggt tttcgtttca
    21961 agtattctgt cagacctaag atctctgctg cattgtccac acgttttttg atgtctgctt
    22021 tatcatattt acgtaatttc aagccaaaag ccatattatc aaaaacagtc atatgaggat
    22081 acaatgcgta gttttggaaa accatggcaa tatcacggtc tttaggtgcc acgtcattca
    22141 tgacttttcc gccgatattt agttctcctt cagagatatc ttccagtccc gcgatcattc
    22201 gcaaagtagt tgactttcca caaccggaag gaccaacaaa aacgataaat tcacggtctt
    22261 tgattgatag attaaaatca gttacagaat aattttctgc attgtcatat tttttatata
    22321 cgtttttcag agccatttct accataatac gttccacact ttctttttta gttttattaa
    22381 ctatttaaag tataaatgaa agcgttaact aacttcaatg tcatgttgaa caagaatttt
    22441 ttcttttttt tgtcaatgtg ctagttttaa gctttaataa atatccataa gaaataaaaa
    22501 ctgatacact ggagtaaata agtatgttaa caatatcaaa aggagtgtct cttatgaaaa
    22561 tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt gaaattagca acagcgtata
    22621 aaacaatatt ggaaaaacat gaactttttg ctaccgggac gactgggcag aggatcacgg
    22681 aagcaaccgg cttgccgatc catcggttta aatccggacc gttaggcggg gatcagcaaa
    22741 ttggagccat gatttcggag gatcagttag atttggtgat ttttcttcga gacccgctgg
    22801 ctgcacagcc ccatgaacca gatgtaacag cacttatccg tttatctgat gtctatgaga
    22861 ttccgttagc tacgaatatc ggaacagctg aggtgctact aagaggactg gaagccggct
    22921 tcctagattt tcgaagaatc gtccatgaaa tagacaagca tccactttca ttttgatgga
    22981 taagcgtatg gaaaaataac gtttaatgtt cgcctatatg tagctataaa agctaaacaa
    23041 actgataata tgcgatataa tacgaacgta ttggataata taaacaataa tattagtttt
    23101 tcgtatttgc agtaaatgtg aaaatatgct atagtttctg tgggaaaaga tcccaaaaat
    23161 aatacagagt agaaaataaa gcgttaagtg ctaagaggat gggatgttgc ctcttggacg
    23221 aaggacgtaa tgttcgcggt tttattttcg cattcgctgc atgatacgat gtagcagctc
    23281 ttaaaggaga tgctaaaaat gaagaaacac cggttagacg cacgtatgat tgctattatg
    23341 ggcttattga ttgcattgat ggtcactttg tcccgactgg tagcaatcga gaccccattt
    23401 attaaaatca gtgtcacatt tattccacaa gtgatcatgg gcattctttt tggtcctttt
    23461 tggtcaggga taggcgcagt tttagctgat ttagtaggaa tggccctctt ctcgaaatct
    23521 gcattcttta ttggatttac attgaatgca tttattgaag gagcaatcta cggattcttt
    23581 ttctatcgaa aagagatcac ttggaaaaat gccattctgg ctacgcttag tgtcacgctg
    23641 atcatcaact tatttttgac accattatgg cttgcgttga tgtaccatgt gccattgttc
    23701 agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc gattcaatca
    23761 attgccattt attacgtagg acgtagtatt ccttacaaaa aaattttaag aagtttagcg
    23821 atccacgcta aataaaaaac aagcagtctt cccatcccag aagactgctt gtttttattg
    23881 tgctgtgaat tgtgcttgat agagccgtcg gtaataacca tctttttgaa gcagctcatc
    23941 atgggtgccc atttcgacaa tagctccctg atccatcact aatatctggt ccgcattttt
    24001 gattgtggac aggcgatggg caatcacaaa actggttttt ccttccatca ttgctaagaa
    24061 ggcttgttgg atttttttct ctgtcaatgt atcgactgag ctggtagctt catctaagat
    24121 caacataggg ggattactga tcatcgttcg agcaatcgtc aacagttggc gttgtccatc
    24181 tgatatcttt aacccttgac cgccaatctt agtatctaat ttttccggca aacgttctac
    24241 aaattcatac atataagagg cttttagggc atcattgatt tctgcgtcac ttgcttccgg
    24301 attcccaaaa cgaagatttt ctcgcaatgt actgtcaaat agccaagtat cttgtaacac
    24361 cattccaaaa cttttacgta atgtatcacg ttggatagag cggatgtcat ggtcatcgac
    24421 tgtaattgtt ccttttgttg tgtcataaaa acgcatcagc aaattaacaa gtgtggattt
    24481 tccggcccct gtttttccta ctatagcaat ggtttgacct ggataagcag taaaattgaa
    24541 atcttcgatc aaacgctgct tggggtcata agaaaaagaa acatgatcaa aagagacttt
    24601 tcctttgata tttttgggtt ccaaggcata ggcatctggc aaatcaggct tttcttctgg
    24661 ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt
    24721 gattccagaa agttcgataa atggtttaga aaactgactt gagtagatcg taaagctgga
    24781 aatcacacca acagtgattg acgaaccact cttcaatgcc aataggccgc caactaaacc
    24841 aatcgataaa taagcaaggt gatctacaaa acgagaagac gggtttgtca aggaagaaga
    24901 aaattgtgcc cgttgtcctt tttcatatag atcagcattt agcagttcaa attgctgttg
    24961 actagcttct tcttgctgaa aagctttcac gattttctgg ttaccgattc gttcggtcac
    25021 aaagcccgag atatccccaa taatcctttg ctgtctatcg aaatttgttt gtgaagcacg
    25081 agcaacgatc caactcacaa ggaaaataat tggtgtagaa caaagaacga ccaatgtcag
    25141 aagtggactt agacgcatca taaaaataaa cgaaagaatg atgattgcca ctccagaaaa
    25201 taattgattg aaggccgcag aaattgcaat ggaaatattg tccatatcat ttgtaaatcg
    25261 actgacgata tttccgtggg agttttgatc ataataacga agtggcaatc gattcagatg
    25321 agcaaatgca tcttttctta attttgcaac ggataagtaa gctaaccgat tgcttaacac
    25381 ttggatgagc cattgactga ctacgttgat taaaacgata ccaccgaata gaataagtaa
    25441 atggaacagt cgattgaagc ggacctgtcc ttctccaatc atttgatcga tactttgacc
    25501 aatttgtaac gtcatcacaa ctgttgcaat cccattgatg attcctagta tcacagctag
    25561 ccaaatgtct tttgcatagg cagtaaggta aggaatgaat cggcggagcg tctgcaagga
    25621 taattttttt actttcataa ctattcttcc tcctgtgatg cgacgatttc ctgatattct
    25681 gctgattgag caagtaattc ttcatgggta ccctgagcag caatcttacc ttgatccatc
    25741 actaagatat gatgtgcttc ttgaatagag cggacacgtt gggagataat aatcactgta
    25801 ctgccaagat aagtctgtag cgccttacgc aaatccaagt ctgtttgata atccaacgca
    25861 cttaaagagt catctaaaat cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc
    25921 agtcgctgtt tttgtccgcc agagaaattt ttcccatttt ccatgacttt tgtatctaaa
    25981 ccatttggaa gctgacggac aaattcttct gcctgagcga ttttcagagc ttcccaacat
    26041 tctttttctg tggcgtcttt cttaccccat tgaaggtttt cacgaatagt cccggtaaat
    26101 agtacagaag tttggggaac aagagcaatt tgctgacgga gatgatccag ctgccaagaa
    26161 cgaacatcca ttccgtccat tataactgta ccggaactga catcatagaa acgcggaatc
    26221 aattgtgtca aagtcgtttt cccgcttccg gttggaccaa tgattccaag gactgtattt
    26281 cgcttcagct gaaatgtaat attttggaga gaaagaccag aatcaggagt ataacggaag
    26341 tcaacatggt caaaagcgat aattgcttca gaagaaaaat cgggagtagt actgacctca
    26401 gcttcaagaa tcgaattttc tgtgtctagc acctctttca cccgatttcc tgaagcttcg
    26461 gcacgtgtga agatcacgac gaggtttgaa acaacgatca gtgccaaaag catttgattc
    26521 atataattga tcaaagcaag aacttgtcct tgttgcaagc taccaatatt tactttgatc
    26581 ccgccaactg tcagcaaaca gatgatcccg acattcatga tcaaggttgt agcaggtgat
    26641 aatagggcag agatattgga aacacgtaaa taattgttcg ctagatcatt tgtgctttta
    26701 tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta
    26761 agattctgac taacgtgttc attgagctga tctagttttt cttgcacttt ttggtagaga
    26821 ggaacggttt ttttgatgat aaaaaataaa atgattgaga aaataggcaa taatgctaag
    26881 aaaaacagac ctacttccca gtcgatcaca aaagccatga ccactgaccc gatacttaaa
    26941 aacggtgctc tgatcaataa acgaatgacc attgctaaag ctagctgaag ctgattgatg
    27001 tcgtttgtca ttcgggtgat caaggtatct gttccaaaat gattcaactc gttgtgagag
    27061 aaagtattga tttttttgat tagctgatta cgtaactctg taccaaatcc ttgtgaagct
    27121 actgaagcat agtattggca aatgatagca caaatcagac caatcacaga catagcgacc
    27181 atccataagc ccatttcaat gatttttcct cggtcattca tttttagtcc attatcgatc
    27241 aaggaagcca tcattaaagg caggacaagt tcgaagacag cttcaagaaa tttgaagaac
    27301 gggcccaaaa tgatttgctt acgatagtct tttgcataac gtaataactg aatcatatga
    27361 ttcctcctat tatttatttg tccacaattg aaaaaacaag tactaccaca ttgtagctta
    27421 ggaaatttag aatgaaaaga gaacaagcct tttcataaat gaaagtttta ttatgaagaa
    27481 gctttagaaa ttgaatagac tatttttttt aaaaaaaaga aaatgagaa
//
LOCUS       DACCIQ010000030        27088 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCIQ010000030 DACCIQ010000000
VERSION     DACCIQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.030"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(298..498)
                     /locus_tag="IVM62_001317"
     CDS             complement(298..498)
                     /locus_tag="IVM62_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6845285.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(803..2035)
                     /locus_tag="IVM62_001318"
     CDS             complement(803..2035)
                     /locus_tag="IVM62_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6845286.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2289..2858
                     /locus_tag="IVM62_001319"
     CDS             2289..2858
                     /locus_tag="IVM62_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6845287.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3036..3476)
                     /locus_tag="IVM62_001320"
     CDS             complement(3036..3476)
                     /locus_tag="IVM62_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP6845288.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3634..4398
                     /gene="map"
                     /locus_tag="IVM62_001321"
     CDS             3634..4398
                     /gene="map"
                     /locus_tag="IVM62_001321"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP6845289.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4430..5353
                     /locus_tag="IVM62_001322"
     CDS             4430..5353
                     /locus_tag="IVM62_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP6845290.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5429..6568
                     /locus_tag="IVM62_001323"
     CDS             5429..6568
                     /locus_tag="IVM62_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP6845291.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6561..7361
                     /locus_tag="IVM62_001324"
     CDS             6561..7361
                     /locus_tag="IVM62_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6845292.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7361..8188
                     /locus_tag="IVM62_001325"
     CDS             7361..8188
                     /locus_tag="IVM62_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6845293.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8166..8900
                     /locus_tag="IVM62_001326"
     CDS             8166..8900
                     /locus_tag="IVM62_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6845294.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9000..9866
                     /gene="rfbA"
                     /locus_tag="IVM62_001327"
     CDS             9000..9866
                     /gene="rfbA"
                     /locus_tag="IVM62_001327"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP6845295.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9880..10452
                     /gene="rfbC"
                     /locus_tag="IVM62_001328"
     CDS             9880..10452
                     /gene="rfbC"
                     /locus_tag="IVM62_001328"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP6845296.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10474..11502
                     /gene="rfbB"
                     /locus_tag="IVM62_001329"
     CDS             10474..11502
                     /gene="rfbB"
                     /locus_tag="IVM62_001329"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP6845297.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11600..12451
                     /gene="rfbD"
                     /locus_tag="IVM62_001330"
     CDS             11600..12451
                     /gene="rfbD"
                     /locus_tag="IVM62_001330"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP6845298.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12486..14519
                     /locus_tag="IVM62_001331"
     CDS             12486..14519
                     /locus_tag="IVM62_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845299.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14563..15843
                     /locus_tag="IVM62_001332"
     CDS             14563..15843
                     /locus_tag="IVM62_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6845300.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16053..16859
                     /locus_tag="IVM62_001333"
     CDS             16053..16859
                     /locus_tag="IVM62_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845301.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16871..18091
                     /locus_tag="IVM62_001334"
     CDS             16871..18091
                     /locus_tag="IVM62_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845302.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18081..19667
                     /locus_tag="IVM62_001335"
     CDS             18081..19667
                     /locus_tag="IVM62_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6845303.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19706..21844
                     /locus_tag="IVM62_001336"
     CDS             19706..21844
                     /locus_tag="IVM62_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6845304.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22212..23195)
                     /locus_tag="IVM62_001337"
     CDS             complement(22212..23195)
                     /locus_tag="IVM62_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP6845305.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23573..24964
                     /locus_tag="IVM62_001338"
     CDS             23573..24964
                     /locus_tag="IVM62_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6845306.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24980..26020
                     /locus_tag="IVM62_001339"
     CDS             24980..26020
                     /locus_tag="IVM62_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6845307.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26039..>27088
                     /locus_tag="IVM62_001340"
     CDS             26039..>27088
                     /locus_tag="IVM62_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP6845308.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTT"
BASE COUNT         8677 a         4536 c         5491 g         8384 t
ORIGIN      
        1 tcgaagggtc aaatttaaac cataattgtg tttttccttc catcagcata aaagttcacc
       61 tcaaaaagag tatacgaacg aatgttcttt ttgtgaaggc ggaatttaat atactgaata
      121 atagaaaaca agtacttgtg ccaaattatg tgccaaaaaa attcgaattc aacaaaatgt
      181 aaacaaatag aaaaccttct atattaagat ttcctgcaat aacaaacaca acagaagacg
      241 tgtaatagtt agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta
      301 ttttgtataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
      361 aagtatattt actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat
      421 aaacagtcga agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac
      481 ggtgtagttg attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac
      541 cagtcctatc caacgaaaga tttatcgttc ttaactctag cggtataact tgaagcttta
      601 cgtaaagcca atgggaatga actggagatg actttttatt atttttagta atagctgatg
      661 ggttaatact tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta
      721 gcgaaataaa ggatctaagc caatagctta gatcctttat tctatgcttt atattatttt
      781 ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt tcggaaaatc ggtactgtag
      841 agccatcttc acggattccg tcgatatcca tcttgtcaga tcctaccatg aaatctacgt
      901 gggtttgact tctattcaat ccagcttctg ctaattcttc atcggacatt tctgtaccgc
      961 ctttcacact gaaggcataa gcagaaccta gtgctaagtg attcgatgcg ttttcatcaa
     1021 ataatgtatt gaaaaagatg atgccagatt gagaaatagg tgaagggtca ggtacaagtg
     1081 cgacttctcc taaacgacgc gcaccttcat ctgtatcaag cagttttgct aagacatctt
     1141 ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt aaaagtaaat ttcatacccg
     1201 agatgatggt gccagcataa ctcagtggct ttgtacttga aatgtaacca tctactcgat
     1261 gactgtcagg tgctgtgaac acttcttccg tcggcatatt tgccatgaat ttttctccac
     1321 gagcattata gcttccagcg ccttcccaaa gatggttttt aggcaatccg ataatgatat
     1381 ctgtaccggg agcagtatag tgaagggcag aaaattgttc tcgattcaat tcttctgctt
     1441 tttttgctaa tttttcatca tgcttcttcc atgctaagac agggtcttct tcatagacac
     1501 gagtcgtttt gaaaatctga tcccataatg cctcaacttg tttttcttct ggcagttctg
     1561 ggaagacttt tgctgcccat tgtttaccag ccgcagccac gacagtccaa ctgactttat
     1621 ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc attcgctgat tgataagacg
     1681 ctactcgatg gctatctaca ccagcaaatg catcgggatc ggcagaaaca acgctgatcc
     1741 tgcttgctcc tttttcaagc cattcatccg cttgatcgat tttagattgt ggtacattct
     1801 caatacgatc cgttgcagca tgtaaaagaa attctctttg gatctggtcg tcagtccatt
     1861 gaacgataac ctcagcggct cctagtttat aagcttcttg tgtgatcaat cgagcaaggg
     1921 gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc tttttctgtg gctacgccag
     1981 tttctgcaat taagcgtgca tattttttta gaagttcatt aaaatcagat aacataataa
     2041 aaattcctcc tcaccatttt ttctggtaac tattagcatg ataccattta attatgattc
     2101 aaacaatgag gctttaaata aaaaaaggag gttatttttt taaggttaag tttcatgata
     2161 tatgttacta cttgctattt cagcttgtga agaaaatcaa aaactaattt tgcatatcaa
     2221 aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat
     2281 taaaaaatat ggcaagaaaa aagacgatta ctagagaaca gattttggca gctgcttatg
     2341 aagtagctgc caccgatgga tttacacgat ttactgcgag aaacatcgcg aacaaaatga
     2401 attgttccac gcaaccgatt tatcttgagt ttaaaaatat ggatgacttg agacaagctc
     2461 tttttgagca gatctatgat catttacaat atgaagtgtt cactgttgag catacaggga
     2521 atacgattat cgatcttgct tttaattata tccattttgc aagaagagag aagaagttgt
     2581 atcgttcact gtgcttagaa gaatatggtg gaggaaaaga gatgcagaat ttctcttata
     2641 cttactttgt gaatacagtg aaacgcgatc cagcttatgt gaatctttca gaagagcaga
     2701 tcgaatcttt gtataatggg acatggatca tagctactgg gatcgcagct ttgatgtctt
     2761 caagcatcat ccatccaact gatgaacaga ttgaaaagat gatccaagac agtatagatg
     2821 ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat atactcaaaa gttccacaaa
     2881 tcagtaggta acaagagatg tgtcaaaata aggaaaagtt cgtttttctt acagcgtatg
     2941 catttttaac tcccgaagaa ctcttccatg aatgcaatga ataaaagagc catacagatt
     3001 gaataaatct ggatggctct tttattgcaa gtatattaaa tttttgcgac tagttcttta
     3061 gcgaaagctt ctaaattttc gatatcttct tcttcagcat tcagatccac tttaacattt
     3121 tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat cgaaatcatc caccgatttg
     3181 cagaattcat cgtaaaaagt atcaccagat ccgcagacac cgtaaatttt tcccaataga
     3241 tccaattctt gcaagtcttc ataaaagtct acgatctcat caggcagatc gccatcatcg
     3301 tatgtataag tagctactac gcaaatatct gcctcctcga attcatctgc atctacttgg
     3361 gtacattcgt tgatttcgac ttcgatatcc agattttcaa atgcttctgc aacaatatcg
     3421 gcgatttctt cagtgtttcc tgtcatgcta gcatatacga tttttgctaa agccatgatg
     3481 tgcctcctcg tttataatta tttaacaatt atgaattata ccaaaacatc attgaaaaga
     3541 aaataaaacc taaagacacc tggggaatgt catttggcta aatgtatgcc gctatgttaa
     3601 aatgagaggg atttgtaaaa aggagacgaa aatatgatta ctttaaaatc agcacgtgaa
     3661 attgaaatga tggcagaatc tggagcttta ttagcagatg tacataaaaa tctgcgtgat
     3721 tttataaaac caggaatcac aagttgggat attgaggtat ttgtcagaaa ctatatcgag
     3781 agtcatggcg gaattgctgc gcaaattggc tttgaagggt atgaatatgc gacttgtatc
     3841 agtataaatg atgaaatttg tcatggattc cctcgtaaaa agcctttgaa aaacggtgat
     3901 ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca tttctgattc ttgttgggcc
     3961 tatgtagttg gagaatctac acctgagatc gaccgattga tggaagtaac gaagaaagct
     4021 ttgtacatcg gtattgagca agcacaagta gggaatcgga ttggtgacat cggtcatgct
     4081 atccaaactt atgtagaagg cgaaaatcta gcgatcgttc gtgattttat tggtcatggc
     4141 gttggtccta cgattcatga agaaccagca gttcctcatt acggagaagc tggaaaaggg
     4201 ttgcggttga aagaaggcat ggttatcacg atcgaaccta tggttaatac cggaacttgg
     4261 aaaatgaaaa tggatcctaa cggctggact gcgtatacaa gagacggcgg actttcttgc
     4321 caatatgaac atacattggc tattactaaa gatggtccaa gaattttgac atcacaagga
     4381 gaagaaggca cttattgatc tatccagtca ataggtacgg gagaggtaca tgaaagtttg
     4441 gaacaaaata aaacaaaatg aaaatctgat gcgctttatt gagacgactc aaaagcgcat
     4501 cactgattca gaaatgagta cgacctctgt cgtcgttgct tattatttac tcttgtcttt
     4561 atttccattg attattgctt ttggtaatat tttaccgttt ctccacatcg atcaagagac
     4621 tgtactgacc tatttacgag aagtgattcc tgagaccgtt taccagttta tcggtcctgc
     4681 aatcaaagac cttttgacac aaagttcagg aggcttgtta tccgtttctg cattagcggc
     4741 tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt gcgatgaata aagcgtatgg
     4801 cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc tcactaattg tcattgtctt
     4861 gttcatgatt gctattagtg gggtaactat cgttcttggg ttgggacagc tgattttgga
     4921 tgctattcag ccaatcgtac aaatacccgt gaactttatc gatcagttcc aaactttgaa
     4981 gtggccaatc acattggtag ctttatttgt catcatgttc ttgatttatt taattgtccc
     5041 taatgctcag ttgaagttga agacgatcat tccgggcacg atcttcgcta ctgtcggctg
     5101 gatgctgtta tcacaagtgt ttagtatcta tgctaggtat tttgcaacga ggatcagcgg
     5161 ttatcagatc ataggaagtt ttattgtact catgctttgg cttaattttg ctgctacgat
     5221 catcattttg ggcggaatca tcaatgcagt tgttcaagaa tacgtgacgg gtcatcaaat
     5281 caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa ttgaaaacta aattttctca
     5341 taaaaaaaag tagaaacaag agttcttatt gtaaatttat atcagtaatt tataataatt
     5401 atgactctta attaaagtag gtgcccatat gcttgtttct tttagtatgg ttataagatt
     5461 ttttctgaca attttattat cactgatatt aacacctgta ttcaaaatca tttcagtaca
     5521 aacaggtatg gtcgacaaac caaatgaaag aagaataaat aaagttccaa tgccttcagc
     5581 gggcggactg cctatcttta tttcatttgt tatatcgact ttattcttat tccgtaaaat
     5641 tatccctcaa tcgtatattg taccgatctt gattgcttct ttagtaatca tagtcacagg
     5701 agtgctcgat gataaatatg agctgacacc taaacaaaaa agtataggga tcttgattgc
     5761 ttctcttatt atttattttg tagcagatat acgtattgat tccttcacgc ttccttttat
     5821 tggatatatc caactaggct ggcttagttt tcccgtaacg attttttgga tcttcggtat
     5881 cacgaatgcg gtcaatctga ttgacggttt ggatgggctg gcggcaggga tttcattgat
     5941 cggtttaatt acgataggaa tcattggata ttttttcctc catgcttcta cagtgtatat
     6001 tccagttgtt attttctgtc ttgtagcaag tattatcggt ttttttcctt acaattttta
     6061 tccggcaaag atttatttag gcgatacagg agctctattc ctagggttta tgatggccgt
     6121 cctttcacta caagggctga agaacgtgac ttttgtttca tcgatttctt tgctgatcgt
     6181 catgggagtg ccggtaacag acacgttttt tgctatcatt aggagaaaag caaaccgtgt
     6241 ttctttttct acagcagata aaaaacatct tcatcaccga ctaatcagtt taggctttac
     6301 tcataaaggt gctgtcctga cgatttatgc gatggctttg atgttttctt tcacagctat
     6361 ggtcatgaat tatactggaa gaatcggaac gatcatattg attattgcca tgttgtttgc
     6421 tgcaatttta ttatttgaat tgattggtct gatcaatgag aaacatcagt ttattttaaa
     6481 aacattgcga tttttaggga ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa
     6541 gaaatggaac cgtcatatag atgaataaca aattaaacca gccgatcctt attagtattg
     6601 tgacggcaaa cagtgataaa atttttcgga cactagataa ttttatttca actgtaaaag
     6661 atgaaacagc agtaaaaatc cgaatttttg acaacaattc acttcccgag tacaaaaaaa
     6721 gactaacaga gtataggaaa tatccctaca tcgatatcta ttttcatgat gaaaattccg
     6781 gatttggtta tggacataat cacaatctga tagctagcga ttctcgctat ggcattattt
     6841 gtaacccaga tattttagtg gatgaagaga cagttacagc attggtcttg ttattgaaag
     6901 aacatccgga atgtgcgatg ttagcaccta agattcttaa tgaagatgga acaactcagc
     6961 acctcatccg tgagcagctg gctgtttttg attacgtttt gcgctttgtg cctttcaaat
     7021 gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga atgccgagga ttgcctactg
     7081 atgctaatag ttatgtacgg atgatctcag gtagttttat ggtagctgat attgataaat
     7141 ttaaagagat aaaggggttt gatgagcgtt tcttcatgta ctttgaagac aacgacttgt
     7201 gtttgagttt tgaaaaagca ggttacaagt tattgtacac acccttttat gcagttatcc
     7261 atttttacgg gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta
     7321 tgatgaaatt tttcaataag tgggggtggc gttttttctg atgaaaccag aaatattagt
     7381 agtcgttgtg ctttatcagc aactattcag tcaatctcca gcttatggac cattaagcaa
     7441 agcacttagc gaaaagcaaa ttcagcttat tatttacgat aatagtcctc aaaggcagca
     7501 aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa atcctggttt
     7561 agcggtggct tataattttg cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac
     7621 tctggatcag gatacgaggc tagtcgacga ttattttgaa ctattgcgta aagtaacctt
     7681 tacagatgaa tgtgttgctg cagttccgat gattttttct gggagccgac aagtttctcc
     7741 cctatattca gatcgttata tcaatcgtac agctcaagca gtagaagttg aaggcccaac
     7801 agctgaacga atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa
     7861 gattggcggg tttaatcctg ctttttcttt agattttttg gatcattggc tgttttggaa
     7921 agtggatcag ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac atgatctttc
     7981 ggttttagac tatgaaaaag tttcaagtaa tcgataccgt tctattttac aagcagaaaa
     8041 tcgattttat catgaatatg atcaaaggca tttatccagt caccgacgac aattattgtt
     8101 aagaacagct aaacagttct tgaccgtcaa aaaccggcat atctggcgga tgactttgcg
     8161 ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta
     8221 atggaggcag atatttaaga gagcagttag atagtatttt atctcagctt tcagcagaag
     8281 atgaagtagt catttctgat gatgggtcag tagatgatac atgggatata ttaactgaat
     8341 acgcagaaaa agataagcga atccagcttt tttcggggcc tcgtcaagga ttgatcgcta
     8401 attttggtta tgcgatccag cagacgaaag gagatctaat ctttttggca gaccaagatg
     8461 atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag aatccggatc
     8521 agctagtagt agtttctgat ttaatcattg tggacagcga gctgcaagag atccatgatt
     8581 cgtatttttc gtttcgaaat tccaaagaag gatggcttaa taatgtgata agaagtcact
     8641 atattggtgc tggtatggct ttccggtctt ctttaaaacc cgatattttg ccgatcccgt
     8701 cagaagtgcc gatgcatgat atgtggattg gtttgctggg aggaaagaag actgggttcc
     8761 taagagaacc gttaactctg taccgaagac atgatttaaa cgcgagtgag attgaaacac
     8821 attctaaatt acggcaaaaa atttcttggc gtgttcatct gattacggcg ttgatcaaac
     8881 gaaaatggtt taaacgttga taaaagatgt ttaaatcctt aatatttcca tgacaaatat
     8941 aacaattgat gagataataa caaagtgaaa tttgaaatca aaaaggagag cagatatcca
     9001 tgaagggaat cattcttgct gggggcagcg gaacacgtct gtacccttta acaaaggcaa
     9061 catcgaaaca attgatgccg atttatgaca aaccaatgat ttattatcca atgtctactc
     9121 tgatgttggc gggaatcaaa gaaattctga ttatttcaac gccacaagat acaccacgtt
     9181 tcaaggaatt atttggaaac ggaaatgatt taggactgca gatcgaatat gcagtacaag
     9241 aaagtccaga tggattggca caagcgttta tcattggaga agaatttatt ggagatgata
     9301 gtgtctgctt ggtattaggg gacaatattt actatggtgg cggtctgtct aaaatgttgc
     9361 aacgagcagc ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc aatgatccag
     9421 aacgctttgg tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc
     9481 ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta tttttatgac aatgaggtaa
     9541 tttctatcgc taaaggtatc aaaccttcag aacgtggaga actagagatt acggatgtca
     9601 ataaagcata tttggataaa ggaaaactat ccgtagaact tatgggacgc ggatttgctt
     9661 ggcttgatac aggaacacat gaatcattgc ttgaagcatc tacctttatc gaaacaattg
     9721 aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt atgggctaca
     9781 tcgataagga acaattgctg gcccttgcac agccattgaa aaaaaatcaa tacggtcaat
     9841 atttattgaa tttggccgct gaataaggag tccttacaga tgaaagtaac agatacaaaa
     9901 ttaacagatg tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg tttcttcaca
     9961 gaaagttatt caaaagcaaa atttgcagaa catggattga aatatgactt tgttcaagac
    10021 aaccattcac tatcagctga agctggtgtc cttcgggggc ttcatttcca aaaaggagaa
    10081 gctgcacaga caaaattgat tcgggtagtc actggagcag ttttagatgt agtagtggat
    10141 atccgcaaag gtagcccgac ttatggtcaa tgggacggat ttatcttatc tgaacataat
    10201 catcgacagt tgcttgtacc taaaggatat gcccatggat tcgttacttt gacaccaaat
    10261 gtgaacttcc tttataagtg tgataattac tataatgcag aagcagatgg gggcatcgca
    10321 tttgatgatc cagatttagc gattgattgg ccaattgatc catcaagagc gattatgtcg
    10381 gaaaaagatc accgtcatcc gacattaaaa gaatttgaag cagaaaatcc atttgtttac
    10441 ggagaaatct aagaattaag gagaaatttt cttatgaaaa acatcattgt taccggaggt
    10501 gccggattca tcggctctaa tttcgttcat tacgtggtaa acaatcatcc agaagtgcat
    10561 gtcactgttt tggataaact gacttatgca ggaaacaaag agaacttggc cggacttcct
    10621 agcgatcgcg tagaattggt cgtaggagat gttgcagatg cagaattagt ggaccgtcta
    10681 gtaaaagaag cggacgcagt tgttcattac gcggcagaat cccataacga taattcatta
    10741 aaagatcctt tcccatttgt acaaacgaat atcattggta cgtatacact tttagaagct
    10801 tgccgcaagt atgatgtacg ctatcatcac gtatctactg acgaggttta cggcgatctt
    10861 ccattacgtg aagacctgcc aggacatggg gaaggcgaag gagaaaaatt cactgcggaa
    10921 acaccatata atccttcaag tccatattct tctacaaaag ctggttcaga tctgttagta
    10981 aaagcatggg tgcgttcttt tggcttacgt gcaaccattt caaactgttc aaacaactat
    11041 ggtccttatc agcatattga aaaatttatt cctcgccaag taacgagtat cttaagcggt
    11101 attcgtccaa aattatatgg cgaaggaaag aatgttcgtg actggatcca tacgaatgat
    11161 cattcttcag cagtgtggct gattttgacg aaaggcagaa ttggcgaaac ctatctgatc
    11221 ggtgcagacg gtgaagaaga taataagacc gtgatggaat tgatcttaga aatgatgggc
    11281 caaccaaaag acgcatacga ccatgtaaac gaccgcgcag ggcacgattt gcgttatgca
    11341 atcgattcga caaaattgcg agaagagcta ggttggcagc ctgagtttac gaacttccgt
    11401 gatgggttgg ctgacacgat caaatggtac gaagaacatg aagactggtg gaaaaaagaa
    11461 aaagaggccg ttgaagcagc atatgccaaa aatggacaat aatacagagg aaatttcact
    11521 gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa
    11581 tcaaggagcg gataaccata tgtttttatt aactggagga aatggacaat taggtacaga
    11641 attgcgccat ttattagatg aaaaaaatct gccatatgtt tcaacagatg caaaagactt
    11701 ggatatcaca gatgctgaga aaacaatggc ttatattaca gaattaaaac ccgatgttat
    11761 tttccattgc gcagcttaca cagcagtaga taaagcagaa gaagaagcaa aagaattaga
    11821 tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc
    11881 acaattcgtc tatatcagta cagactatgt tttcgatgga aaaaacaaaa atgtagaata
    11941 cgaagtagac gaccaaacga atccactaaa tgaatatggt cgtacgaaat tattaggcga
    12001 aaaagcagta gaagagattt tagaagacta ttatatcata cgaacttctt gggttttcgg
    12061 gatctatgga cacaatttcg tcttcacgat gcaacgccta gcggaaaccc gcgacaaact
    12121 gactgtagta aatgaccagt ttgggcgacc aacttggaca cgtactttgg cagaatttat
    12181 ggtatatgtc atcgaagaaa aagcaccatt tggtatttat catttgtcga acgaaaatag
    12241 ctgttcttgg tatgagttcg caaaagaaat attgaaagat acagatgtag aagttgcacc
    12301 tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg cagtattcag tcatgagcct
    12361 gaaaaagaca gaagcgttag gttttgtcat accaacttgg caagaagcac ttgcgcaaat
    12421 gctggaagct gccaaaaaaa tgaataagta atttcctttt tatcaaatag aatttggagc
    12481 atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaatcagg
    12541 cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag
    12601 tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat
    12661 atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag
    12721 tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact
    12781 tttcgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt
    12841 ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg
    12901 gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg
    12961 gattcgaagt actgatgata ttaacgaaga ggaaaaaagc tctatcgcta atcggtgcat
    13021 ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga
    13081 ttttctttac tttgggactg atgattgctt tttatcatta tttctacaat catgataaaa
    13141 aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag
    13201 tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc
    13261 tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag
    13321 gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat
    13381 tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga
    13441 cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact
    13501 attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa
    13561 gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga
    13621 tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag
    13681 aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg
    13741 cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt
    13801 ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt
    13861 cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag
    13921 tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct tttgtcgcct
    13981 tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg
    14041 aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt
    14101 tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag
    14161 tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa gaatacgaga
    14221 agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca
    14281 ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag
    14341 ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc
    14401 agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc
    14461 cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat
    14521 aggctagctg agaataatcc tgaaatatta gtagaaagga gagtgaagat aattcaagca
    14581 atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta
    14641 gtcttttttg tttggacagc aggggtaggt tttccagcaa ctttgtttat atataacaat
    14701 agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca
    14761 aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac
    14821 tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt gattatttta
    14881 ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt
    14941 gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca
    15001 gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat
    15061 acaatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt
    15121 gaacgatggt atgttcgaat catcggtggg atagcagtcg ttgcagcctt tttaggtatt
    15181 tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt
    15241 ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcctacac ggttacgatt
    15301 cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc
    15361 gatcgagaag atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt
    15421 ttactaggaa tgaaaacatt tatcttccag cgaggaaacc aatttttagg aaaccacaat
    15481 agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc tgctttgctg
    15541 cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact
    15601 tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca
    15661 tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa
    15721 ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat
    15781 gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca acgctatcat
    15841 tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggtttattg
    15901 caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta tcatagaaaa
    15961 ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg cacttgactg
    16021 taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat ttatttatga
    16081 aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac ttcaaatcaa
    16141 gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta tttactgtat
    16201 tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc
    16261 catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca gatgcatttg
    16321 catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc gtattcaata
    16381 ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccattta ttcttcattc
    16441 tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg tctttacagc
    16501 tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg ctgacggcat
    16561 cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg cagttgatca
    16621 tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt gtaagcatat
    16681 tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt ttaagtgaag
    16741 cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg ttagtaatgt
    16801 tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat gtgctgtaag
    16861 gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat cttataatat
    16921 gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga aatcctatca
    16981 tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg
    17041 tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta cgggagtact
    17101 gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc tagaactagg
    17161 atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg gaatggttct
    17221 aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat ttgctgacat
    17281 cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc
    17341 ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag cattggcagt
    17401 aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca agaactcggg
    17461 aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc
    17521 ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag taacgacgaa
    17581 ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata aaaaccaagt
    17641 agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt tggataaaga
    17701 caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag taacttatga
    17761 agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa
    17821 tttttatgta tgcggactta atactctact agatggtatc aagattcctt ggaaaaaagg
    17881 agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg aatattattt
    17941 tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga caaagtacat
    18001 gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag accataaatg
    18061 ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat gaacaaagca
    18121 gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag tttggtcccg
    18181 gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg agcattgttg
    18241 agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga ttttatggtg
    18301 atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat tacgtcttgt
    18361 ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa gtcagagaat
    18421 ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat aattctgtca
    18481 tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta gatgaaaccc
    18541 ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg ggattattca
    18601 ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa tcaacttatg
    18661 aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt gaagtctacc
    18721 aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca gagccacaaa
    18781 attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc
    18841 aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc cccgtatcag
    18901 aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc gaattcagcg
    18961 cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa
    19021 atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg gatatttctg
    19081 ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca ccaacaatga
    19141 aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa
    19201 atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga ctagaccata
    19261 cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa tggacaataa
    19321 aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt
    19381 ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa atcatcggat
    19441 ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct gaccaatcgc
    19501 catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa
    19561 aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt tcattcttag
    19621 tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata atgattaact
    19681 tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca
    19741 aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt
    19801 gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg aatcaacaca
    19861 tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc aaagcggttt
    19921 caaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc aaacagcgag
    19981 acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg acggcattga
    20041 atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg caaggactca
    20101 tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat cgatcgacta
    20161 tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga aagaagcaca
    20221 aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg ttgaaatcaa
    20281 atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt gggaactatg
    20341 tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat cctatcaagc
    20401 aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca gtctggcaac
    20461 taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata atgatgatga
    20521 attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc cagaactgga
    20581 tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg atccgcattt
    20641 caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc acttaggcgt
    20701 atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg aaggttctca
    20761 agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata tttaccacat
    20821 tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta atggcgaagc
    20881 caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt ctagaagaaa
    20941 catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg cttatgacgt
    21001 cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg aagatctaaa
    21061 aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa tcattattgc
    21121 ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca agcatcagct
    21181 gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc gaattaacaa
    21241 tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg atacagaagt
    21301 gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc gaatcggctg
    21361 tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag tattattagg
    21421 aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt gtggttattt
    21481 cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta tgatggtcaa
    21541 ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag cattcaacga
    21601 tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg cacatcaagc
    21661 agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag aaaaacaaag
    21721 acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata ttgcgcatga
    21781 tccattttac aatgataatt taacaaaaga cggaatcggc gacttttcgt tgagaccaga
    21841 ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg ctttttatag
    21901 ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag gttgggacag
    21961 aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg
    22021 aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg tttttaaaga
    22081 atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca gagcgccaat
    22141 agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc gatattttat
    22201 tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa agccactcta
    22261 tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt cttagcgtca
    22321 atataaagaa catttccact attaccaatt gctgaatata tttcgttctc agaatctaat
    22381 acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact ggttctaggt
    22441 ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata catcccttta
    22501 tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa accatatcct
    22561 gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga cttatccatt
    22621 gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt tggtatccct
    22681 ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact tgtctgatac
    22741 aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc ggttatacct
    22801 tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga acagactttt
    22861 gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg cttgattgta
    22921 atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata atctaatact
    22981 ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag caaagataca
    23041 atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc attccattca
    23101 tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt tggttgtcga
    23161 aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac tttcttctat
    23221 aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta atacaggtat
    23281 tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata aaggttcact
    23341 ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc aacattagtg
    23401 aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag aggtgtataa
    23461 agctttttat tagacatttt taaattccat tacaatttgc ttataaacta ggtttttaaa
    23521 ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat atatgaataa
    23581 aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg ttattgtata
    23641 caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc cattacgaaa
    23701 tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg cgttgaacat
    23761 tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt ttgtcacagt
    23821 catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg gccgctggtt
    23881 tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt tcttaagtat
    23941 ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa aggtcgtcat
    24001 tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa
    24061 caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca aaaatatgat
    24121 taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa aaatcaaaat
    24181 ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat ttgaaaactt
    24241 gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg aagcatctgg
    24301 atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca tcgtttcact
    24361 tgttttatta gttattgctt caccatttat gttattcaca gcaattttag tcaaagtgac
    24421 atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa aggaattttc
    24481 aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac cagttttagc
    24541 taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg tccgctttga
    24601 tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag gaccacgtcc
    24661 tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact acttacggca
    24721 taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg tctctgatta
    24781 caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga tgcttgattt
    24841 aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc aaggacttga
    24901 agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa aaatctttcg
    24961 ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac cagtatacaa
    25021 tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt atgaagagta
    25081 cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca ttcaagaact
    25141 gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc ccggattagc
    25201 aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg atgcagatga
    25261 cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga gtccaaaagc
    25321 ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa tcgtttccga
    25381 aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta taaaagacat
    25441 gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct atcgtagcga
    25501 tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg atcgaatatt
    25561 taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag tagaatatat
    25621 ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca agttcgaaac
    25681 atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata aagacggtat
    25741 ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata gtacctctgc
    25801 aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc cagtgatccg
    25861 agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa agttgcttta
    25921 caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg ttgaagtgaa
    25981 aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg aaaatataat
    26041 gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt ctttcatgat
    26101 gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga tttatgaaga
    26161 ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc gtatttttgt
    26221 tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata agttactaaa
    26281 aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa ttaatttatg
    26341 gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa ctgaagatgg
    26401 aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat tgttgttaca
    26461 aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt ttcaaaacca
    26521 tccctttgag atagttcata atggtattga tttagagctt tatagtaaaa attcagaagc
    26581 taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg tgggaaacat
    26641 tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt tagaaatact
    26701 aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta gaaataaact
    26761 gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag gagaaagaaa
    26821 cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc tttatgaagg
    26881 tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct tagcagatac
    26941 tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag atgaaacacc
    27001 acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa cacaagaact
    27061 aaagagttat gatgtttcgt acactact
//
LOCUS       DACCIQ010000031        26790 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCIQ010000031 DACCIQ010000000
VERSION     DACCIQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.031"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IVM62_001341"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IVM62_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP6845309.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="IVM62_001342"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="IVM62_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP6845310.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="IVM62_001343"
     CDS             complement(2738..3049)
                     /locus_tag="IVM62_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP6845311.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="IVM62_001344"
     CDS             complement(3046..3339)
                     /locus_tag="IVM62_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP6845312.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IVM62_001345"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IVM62_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP6845313.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IVM62_001346"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IVM62_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP6845314.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="IVM62_001347"
     CDS             complement(5177..9529)
                     /locus_tag="IVM62_001347"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP6845315.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="IVM62_001348"
     CDS             complement(9741..11450)
                     /locus_tag="IVM62_001348"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP6845316.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IVM62_001349"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IVM62_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP6845317.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGITTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="IVM62_001350"
     CDS             complement(12947..13747)
                     /locus_tag="IVM62_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP6845318.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="IVM62_001351"
     CDS             complement(13744..14556)
                     /locus_tag="IVM62_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP6845319.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IVM62_001352"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IVM62_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP6845320.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="IVM62_001353"
     CDS             complement(15311..16033)
                     /locus_tag="IVM62_001353"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP6845321.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="IVM62_001354"
     CDS             complement(16169..17050)
                     /locus_tag="IVM62_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP6845322.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IVM62_001355"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IVM62_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP6845323.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="IVM62_001356"
     CDS             18290..18769
                     /locus_tag="IVM62_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP6845324.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="IVM62_001357"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="IVM62_001357"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP6845325.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="IVM62_001358"
     CDS             20070..20855
                     /locus_tag="IVM62_001358"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6845326.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="IVM62_001359"
     CDS             complement(21201..22892)
                     /locus_tag="IVM62_001359"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP6845327.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IVM62_001360"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IVM62_001360"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP6845328.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IVM62_001361"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IVM62_001361"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP6845329.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IVM62_001362"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IVM62_001362"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP6845330.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7127 a         5790 c         4769 g         9104 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta taccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccatat
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc
//
LOCUS       DACCIQ010000032        26574 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCIQ010000032 DACCIQ010000000
VERSION     DACCIQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.032"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(324..893)
                     /locus_tag="IVM62_001363"
     CDS             complement(324..893)
                     /locus_tag="IVM62_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6845331.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IVM62_001364"
     CDS             complement(967..2256)
                     /locus_tag="IVM62_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845332.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IVM62_001365"
     CDS             2610..3458
                     /locus_tag="IVM62_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP6845333.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IVM62_001366"
     CDS             3472..4545
                     /locus_tag="IVM62_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP6845334.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IVM62_001367"
     CDS             4535..6706
                     /locus_tag="IVM62_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP6845335.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="IVM62_001368"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="IVM62_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP6845336.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="IVM62_001369"
     CDS             complement(9117..11303)
                     /locus_tag="IVM62_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP6845337.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="IVM62_001370"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="IVM62_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP6845338.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="IVM62_001371"
     CDS             complement(11525..11965)
                     /locus_tag="IVM62_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP6845339.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="IVM62_001372"
     CDS             complement(12039..12512)
                     /locus_tag="IVM62_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAP6845340.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12822..14288)
                     /locus_tag="IVM62_001373"
     CDS             complement(12822..14288)
                     /locus_tag="IVM62_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845341.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14599..16683)
                     /gene="cadA"
                     /locus_tag="IVM62_001374"
     CDS             complement(14599..16683)
                     /gene="cadA"
                     /locus_tag="IVM62_001374"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6845342.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16825..17013)
                     /locus_tag="IVM62_001375"
                     /pseudo
     CDS             complement(16825..17013)
                     /locus_tag="IVM62_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17207..17566)
                     /locus_tag="IVM62_001376"
     CDS             complement(17207..17566)
                     /locus_tag="IVM62_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP6845343.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17596..17937)
                     /locus_tag="IVM62_001377"
     CDS             complement(17596..17937)
                     /locus_tag="IVM62_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845344.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17934..18608)
                     /locus_tag="IVM62_001378"
     CDS             complement(17934..18608)
                     /locus_tag="IVM62_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6845345.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19404..20702)
                     /gene="asnS"
                     /locus_tag="IVM62_001379"
     CDS             complement(19404..20702)
                     /gene="asnS"
                     /locus_tag="IVM62_001379"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP6845346.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20742..21932)
                     /locus_tag="IVM62_001380"
     CDS             complement(20742..21932)
                     /locus_tag="IVM62_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6845347.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21953..22480)
                     /locus_tag="IVM62_001381"
     CDS             complement(21953..22480)
                     /locus_tag="IVM62_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6845348.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22527..25316)
                     /locus_tag="IVM62_001382"
     CDS             complement(22527..25316)
                     /locus_tag="IVM62_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6845349.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25463..25663)
                     /locus_tag="IVM62_001383"
     CDS             complement(25463..25663)
                     /locus_tag="IVM62_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6845350.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26115..26435)
                     /locus_tag="IVM62_001384"
     CDS             complement(26115..26435)
                     /locus_tag="IVM62_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845351.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7471 a         5626 c         4665 g         8812 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
    12661 agctgacctt cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc
    12721 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga
    12781 agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt
    12841 tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct
    12901 aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta
    12961 ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag
    13021 gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca
    13081 gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa
    13141 cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct
    13201 ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa
    13261 ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga
    13321 tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt
    13381 tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa
    13441 ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta
    13501 tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt
    13561 gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca
    13621 taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa
    13681 taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat
    13741 tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg
    13801 acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt
    13861 gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac
    13921 atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct
    13981 tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat
    14041 gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc
    14101 ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa
    14161 atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt aactacattc
    14221 cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg
    14281 ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa
    14341 tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat ttattacaca
    14401 tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt tcattgacct
    14461 tattgaattt caagctattt tgaattttct gcaaaattaa aaacattagt tattttataa
    14521 tcctatctat tttctttaca tttttcatta agaaaaggat ataaaaaaaa gaggacgttc
    14581 attcgtcctc ttcattgatc aacgtttatt ttttacatgc aagcttcgca tggcattcaa
    14641 aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg atgcaaaacc
    14701 aaaagcacct aaggaaagta ccaaaatttt tatcccaata gcaaagacaa tgttctgttt
    14761 gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg atggttgatc
    14821 attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc cacccattgc
    14881 gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc cgacaaatgc
    14941 ggtcttttta ttgttacttt gtaagatttc ctctaaccgc tgcactttat cttctggaag
    15001 caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag caattttttt
    15061 cgagtcgccc gtcaacatga ctgtgttttt cacaccagcc tgttttaatt gaaccaatgc
    15121 gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt aactttgatc
    15181 gatcgctaga taaagcaatg taccgatttc ttgacttgaa ggaaaagaga tttcaaatct
    15241 ttccatcaat ttgtgattac ctactaatac ttgtttgcca tcaacttcag ctaagatccc
    15301 ttcacctgca aattcctgga tattagctgt tggtaaaagc ggacctgcgt agccttctaa
    15361 aacagattga gcaatcggat gtgtagaaaa ctgttctgca cttgcaacat attgcataaa
    15421 tttctttgga tcaattcctg ttggatcgat cgtctggatc gaaaagtcac ctttcgttaa
    15481 tgtgccagtt ttatcgaata ccactgtctc agtttgtgca agcaactcca agtaatttga
    15541 accttttact aatacaccga ttttacttgc tcctccgata ccgccaaaga aactaagtgg
    15601 cacggaaatc actaatgcac atggacatga gatcactaaa aatgtcaatg ctcgatagat
    15661 ccattcagaa aacgccgctc tagaaacaac aagtggaggt aaaacagcta ataaaacggc
    15721 caatcctaca acgactggtg tgtaatagcg cgcgaattta gtaatgaaat tttcagctgg
    15781 tgctttttta ctgctggcat tttctactaa ttctaataat ttgctgatag tagaattttc
    15841 ataagatttt tctacttgaa ctaaaaatgg tttattttta ttgataaatc cgcttaatac
    15901 tgtttcacca acatatacag atttagggac tgattctcct gtaagtgctg acgtatcgac
    15961 taatgattct ccttcgatga tcgttccatc taacggtaca cgttcaccag gcttcacaag
    16021 tacaaactga ccgatcagca cgtcttcagg gttcacggtc atcaatcctt ccgccgtctg
    16081 cacgtgagct gtttctggac ggatcgccat caattcgcga atcgattttc ttgaatgact
    16141 gactgccaat ccttggaaat actcgccgat ttgataaaat agcatcactg ctacggcttc
    16201 tggatattca ccgatcaaca tagcgccaat cgtagcaaca gacataagaa aattctcatc
    16261 aaagattttt cctttgacga tattcatcac tgccttcttc actacatcat atcctatcag
    16321 cagataaatc aataaatacc ctatcaagcg aattggttca ttaaaatcaa ataatagcaa
    16381 taaagcgata cctgcaaggg aaaataaaat ccggaacagc gtatttctct ttttttcatt
    16441 tccatgttca gatacttttt ccttatctat ttctgttact ttgacatctg gttctaattc
    16501 cttcactact ttttttgttt cttgttcaat cgtagccaaa tgatcttcct ctgcgtcaat
    16561 cgttagtttt ccagaagtga agtttaccgt tgcttccttg actccattga ttttctgaac
    16621 tcctttttcg attttcattg cacagttcgc acaatctaaa ccttctaatc tgtaactttt
    16681 cattttaatg acctcctcgc ctcaacatat gaatatctct tcataactaa ttataaatga
    16741 atggttattc atatgtcaag gtatccgaaa gaaaaaatga aaaacatctg catcggaaaa
    16801 tttcttccat acagatgttt tggcttattc tttctttttc tcttcgatca ttgccactgc
    16861 tcttatccaa cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc
    16921 tgttgctggc aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat
    16981 ccctgcaaaa tgtccttccc agatatacga agctccacta cgctttcaaa aatatagaaa
    17041 gagttcttta atgaaaatta tttcaacttt ccataaacag taaccccata taaataaaag
    17101 acgtaaaagt cacatagaag aacttataca ggaaaacgca tagagtcagg tgaaatacat
    17161 gtatcccacc tggctctatg ctttatctaa cctttattat tctcttttat acaatttctg
    17221 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
    17281 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
    17341 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
    17401 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
    17461 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
    17521 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
    17581 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
    17641 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
    17701 tgtgtgttgg attagttttc ctgatggtcg ctgatccaat cgttcttctg actgttgcac
    17761 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
    17821 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
    17881 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccttattt ccatcatttt
    17941 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcgac aattgcatct
    18001 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
    18061 gtatatttcg aaaaaccata agcaatccct aaagcattga atataccatc taaaggaacc
    18121 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
    18181 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
    18241 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
    18301 tgagcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
    18361 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
    18421 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaattccatc
    18481 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
    18541 gttgcttctg ctcggtatct tcctgcgtct gtaatgctat caccgataaa taatatacgt
    18601 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
    18661 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
    18721 tccttcaaac aattgaatga acgctcatca agtgtctagt tttttatgct atttttttct
    18781 taacgtgccg gattcccctt gattccttcg ttgtttggtc cagagagatc gatcacaccc
    18841 aagcttccgt caatcagacc actgatgacg ccatcgaaac atatatttat aggctcatct
    18901 aagacttcta gtaaatgtcc ctttactaat atctcttcta gattgcttgg gatagattgg
    18961 tcataacttt atctggtccg atgatttcaa gggtgaattg aacttacata tggattattg
    19021 catgctgaaa atacggacct actccatctt cgaatgttac tattagtttg ttttcaggtg
    19081 ctagatgctg ctttatttca acagcagaag catcatcaaa tcagatttcc atttgaatta
    19141 ccttctttgt aaatgtttga ttgattgcga tttcgttata gaaccttgtg aatcaaatag
    19201 caatttcttt tgctttcttc tcttgtctgc tggaaacttc cattatcccg tatttaaaaa
    19261 aaaagaactg cgagagttcg cagttctttt ttttaaataa acgttgctct ccggccacaa
    19321 gtcacaatag tcatcaaaca tgaaaaactg ttttttgaaa ttactccttg tgactcggag
    19381 ctgaacggca ggcgcacaac ctcttatgga taaattcggt tcaacaaacg tgggaatgga
    19441 atagcttcac gtacatggtc gataccagca atccaagtga ctgttcgttc aagacctaga
    19501 ccaaaaccag agtgcggaac agaaccgtat ttacgtagat caagatacca tgaatattct
    19561 ttttcatcca aaccgtgatt acggatttgt tctagcaaga aatcatagtc cgttgcccgt
    19621 tcgcttccgc caataatttc accgtaacct tctggtgcga tcatatctgc acaaattacc
    19681 acgtcttctc ttgttggatg tggtttcatg tagaatggct tgatggcttt tggatagttt
    19741 aggataaaga caggctgttc gaatgaattc gcaatgaacg tttcatgagg tgaaccaaag
    19801 tcttcgcccc attcgatgtc atcaaatcca tttttcttca gtaattcgat tgcatcatcg
    19861 taagtaatac gagggaatgg caattttgta taagttttta agacttcttt gtctcgtcct
    19921 agtacatcta atgcgtattc gcaatgatct aaaacatttt gtactaagaa tgctacatat
    19981 tgttcctgta cttctaaact ttcttcttga tgagtgaaag ccatttctgg ttcgatcatc
    20041 cagaattcga tcaaatgacg tcgtgttttt gatttttcag cacggaaagt cggaccgaat
    20101 gaaaagactt ttccgaaagc catagcagca gcttccatgt ataattggcc actttgagat
    20161 aaataagctt tttgatcaaa gtaatttgtt tcgaatagat ccgtagtgcc ttctggtgca
    20221 gaaccagtca agatcggcgg atcaattttg acaaaatgat gttgattaaa gaattcatac
    20281 gttgcacgaa tgatttcatt tctgatctgc ataatcgcat gttgtcttga agaacgcagc
    20341 cataaatgac gatgatccat caagaagtct gttccgtgtt cttttggtgt gatcggataa
    20401 tcgtggctct cgcttaccac ttccacattt gtaattccga tttcataacc aaattttgaa
    20461 cgactgtctt cacggatttc tccagttact aacacggatg tttcttggtg tagattttta
    20521 gctgtttgga aaacttcttc cgacacttcg ctttttacaa ctacaccttg gaaaaaggct
    20581 gttccgtctc ttaattgtaa aaatgtgatt tttccgcttg atcgcttatt tgcgacccaa
    20641 gcgccgattg ttactgtttc tcccacatgg tttttcgaat cgatgatttg aatttgttcc
    20701 acgtatgact ctccttttat attccttgaa tttcttcagg attaattttg acttttactc
    20761 tcaataaatt gcttgattct gcgtaccgct tcttctaacg tagcgcgatc agtagcataa
    20821 ctcatgcgga cattttctgg tgccccaaag ccttcgccag tcacaagcgc tacccctgtt
    20881 tcttctaaaa gatcttctac ccattgtgtg acattttcat atttacacat gcgtagcgtt
    20941 tcttttacat taggaaaaag atagaaagct ccttgcggct tttcaagaga aacacctggc
    21001 agttctgcca caagcggata taacgtattc aggcgttctt caaaggcgtt ccgcatttct
    21061 tctactgtat cttgctcacc tttcaatgct tcaacagctg catattgact gactgctgtc
    21121 ggattactgg ttgactggga agcaacagca atcattccat tgatgatctc agaattcccc
    21181 accgcataac caatgcgcca gcctgtcatc gcatatgttt tagacacgcc attgatgatg
    21241 atggtctgat ttttgattgc ttctgaaatc gttgcaattg gagtaaactc attgccatta
    21301 taaactaaac gaccataaat atcatcagca actatcaaaa tatcattttt tactgcccat
    21361 tcaccgattg cctgaagttc attcttgcta taaatcattc cagtcggatt cgatggagaa
    21421 ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt ctagctgttc aacagtaact
    21481 ttccagctat tggattcttc ccctttcaca aagacaggaa gtccttctgc cagttttact
    21541 tgctcaccgt aactgaccca gtaaggcaca ggaatgatga cttcatcttg cgggtcaagg
    21601 atcgtttgga aaagggtata caaagcaaat ttcgctccgt ctgttacgac tgtttgtgaa
    21661 ggttcataga caagtccata gtttttcttc agataatcaa cgattgcctg tcgaagctct
    21721 gggattcctg ctgttggtgt ataatagcta gctttcccat tgcggattgc ttcgattgct
    21781 gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc ccacagtcaa actcagcaca
    21841 tctctgcctt ttgcttttaa tgccttggcc tttgccgcag ctgccaaagt gacagacggt
    21901 tctaatcgtt ttgttctttt tgataactcc atcccattca ttcctttacc ttttaaattc
    21961 ccttgattgt cttgacttca tctccagatt caaagccaat caggtaataa ttcagtttcc
    22021 catttgtttc ttttcctact acttcccaca caggctgatc atcatacatc cctaaagcag
    22081 ctttttcgat ttggatttca ggatgggccg cagcaattgt ctgttttgct tcgttttcag
    22141 tcagtccctt tgtttgatcc aatactttta ctttttcacc agatttagga atgataacgg
    22201 cgatttcttt tcctttatca tttgtacctg tcagtgaaaa gtatgtcttc tttcgagtaa
    22261 accaataaaa atgatcggct tcctcaagat ttgcatattt ctttgcgatt tctactgctt
    22321 ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta aaaaatcgag ctgatcacga
    22381 tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg attaatcgtt ttttcctttg
    22441 tctgtttttt cttcaagggt cgtcgctcct ttatcttcat tccttttcat tatagcagat
    22501 tttaattatg aaaaaagaca gctcctctaa cttccttctt catttttctt taagaattca
    22561 tggatgtcct gatagatttc ctccatagct agctctttga tcggtacttt tttcggtaat
    22621 gcatggatga tccgtgcacc atattttgca gtgactagtc ttcgatccaa aataagcaat
    22681 acgccacgat ctgtctccgt ccttatcaag cgacctaatg cttggcgtaa cttcaaagct
    22741 gctttaggta cggattcctg gtaaaaagga ttgatcccct ctgattctaa ataagcgttc
    22801 ctagctttga ccatcgggcg aagcggatta tcgaagggta aacgtgtgac tacaacgatc
    22861 tgcaaagcat ccccaggcaa atctacacct tcccaaaagc tgtcagcgcc aaacaatacg
    22921 cttttttctg acaaaatgaa gcgtttcaaa agcttctccc ggcttccgcc aaacccttga
    22981 gctagtattt ctcgtccttt ttctagcata gacagatgca ttttctgata gaccttctgc
    23041 aaaacatcat gagaagtgaa taacacaagt attggccgat cttcttgttc gatcagacct
    23101 gtaagtgctg tctctaaata atcgatatat gcttgattgc tgacttgatg gacggcaggt
    23161 gcctcttttg gcacatagat ccttgcctgt ttctcataat cataaggaga ggcaatcact
    23221 ttaagaggtg ctgcttcaat gccaagccgc ttagagagat agtgtcgatt agggccgatc
    23281 ttcagcgttc ctccaatata aagaatacga tcatatcgag gataccaaac agtcgacggc
    23341 aacatagccg cttgaaaatc atggagatga aaaatcatct gctgcgctgt gttcccagga
    23401 acgaaccaat ggatcaaatg atctcgccag tcttccagcc attgatccat cagcaagtac
    23461 tgcttgtcta attgatcaaa taagctatat aaacttgcta aatcgatccg catctggttt
    23521 gtccagcttt cttggataat ctcaaaagtc agacgcattt gtgactgtag ctgctggatc
    23581 tcctgataaa aaagctgcag gtgatcaatg gctgtcccag attccagtgg cagctgatcc
    23641 aacatctctt ttgtcatcat gacttcttct ggatactgtc cgttaggttg cttgccgctc
    23701 actaaactca ttaatccttc tgccatacaa atctgtgctt cgcatagcgc agaaagttct
    23761 tctcgataaa tcgataacca gcgtaaggct tcatgttctt tcggtaaata gctttgtatt
    23821 cggtcaaaca gctgatttgg ttcttctaat tgctggattt gtttatggaa atagttaata
    23881 cttaatcgtt tgtcatttac tttttcagcg atttccggca aatgatgtac ttcatcgatc
    23941 aacaaaaatg gactagctgg aagcaatggt tcttttcgct gtgtttcttg tattaaaaac
    24001 gcatgattga caatcaaaaa attactttgc ctgattttct tttgcaagaa acgaacaaag
    24061 tcttgttcgt aaaatgactg atttcttgct aaaaatccaa tacctctgtg cgcgatatct
    24121 gcaaagaatg aatggttcaa atttgttaaa tgtaattcat ccaaatcacc ggtcttcgtt
    24181 tgcgtcagcc aaactaagac cgccatttga tacaaagcat attgtttttg ttctggcggt
    24241 tccacaagtg tgcctttaaa acgctgtaaa tcaatatagt ggcgatagct tttgataatt
    24301 acaggttgta tgggctgatc tagcaattga ttcagcaatg gaatgtcttt ttctatgatc
    24361 tgctgctgca aaactagcga aacggtactg atgacagcgg gtttttcagg tgtagccaaa
    24421 taactcaatg gcagcagata accgattgtt ttacccattc ctgtagctgc ttcgatcatc
    24481 aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg cattcatcag tctgttctgt
    24541 tcttttcgat acatcagtgt tttaccaaac attttttctt ttgcttgttt ttttcttgga
    24601 tatgtccgct cgccataatg gacggatgtg aatagttcca cttctttttt ctgcaaagct
    24661 agcccatcga cgatttctaa tgagggatca agtggctctg gtttttcttt catttcttca
    24721 atcacatgat aaatgaaacg actcgtatcc atacctgtgt gtctacttaa tcgcgcaatc
    24781 tgctctaacg tgatgattgg caattttctc atccgttctt cgatcagcaa aagaagttga
    24841 cccgtcactt cggcatcact atctgcctga tgaggattct catgaataaa tccaaggctt
    24901 tctgacaaat cagctaagcg aaagcttggt tccgtcggca agaaaatttg tgcaagttct
    24961 actgtatcga tgcctggtat cttcaaacta ggaagtccgc atcgcatgag ttcatggttc
    25021 aaaaaattat aatcaaaata aatattatgc gcgacaaata tggtgtctgc caaaagattg
    25081 taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt ggacgcgttg attcgtgatc
    25141 cctgttaaat gctggatttg ttttgaaatc ttccgccctg gatgaatatc gatcgaaaaa
    25201 cgtgaagtga tccgacctga ttcaatcatg acgcagccaa attggatgat ccgatcttct
    25261 tttggattcg tcccagtcgt ttcgatatca acgactgcat aagttcttgg tttcaaaata
    25321 tcttcctctt ctcctgcctc taatgttctt ttgaaattat actacaaagt gcagaaaaat
    25381 actctcatcg ctttttaaag aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg
    25441 tttatttttt agtataggtt aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt
    25501 ggccttcttc aacatcgaaa gttactgctt ggccttcttc taaagttttg aatccgtcgc
    25561 cttggatagc tgagaaatga acgaatacat cgtttccgtt ttctgcagtg atgaatccaa
    25621 atcctttttc tgagttaaac cattttactg tacctgtttc cataataaaa atcctccttg
    25681 tgtatcaaca catattttgc aattacttga atggtgtata tctggaagat gtttatttga
    25741 aacaagctac cacagattac cgaacaaaaa ctactcctat agtatatcat accgtttaca
    25801 aaaaagctag agtttttcga ctttttttat taaaaaaagt tttctcagcg attacataaa
    25861 gtgaaaaacc gaactttttt taaaataaac gacatattgt tcagttggtg tttttttgct
    25921 ctgatgttct tctttaaact aaaaaaatta ttctttctga ctacttatcg gttctgtttc
    25981 aacggtcgag gctggggaag attctttagt aggtctgatg ttctcatggt atttctgata
    26041 gcctatgaca gttaccagaa gaacaaggca aaccaatacg atcaacgaga ttagtttttc
    26101 ttttttgctc atatctatac ctcataaata acaggatgat gtccatcata tttctgagta
    26161 tgagtaaaag caaaagctgc agctgtagaa tgtaagaaat gccagcaact ttcataatca
    26221 caggtcggac agtaccaaaa gtaaccaaat cttggctgca aagaccgttc ttctgtttca
    26281 ggaatcgcag aatcaagatc tgttgtctgt acataaacat ttttgtacag acgtgtgcct
    26341 gtaataggag accgctgtgt ttcttcagca aaaactggct gacttgttaa acttagggat
    26401 agaaacagca aacaccataa cagtcttttt ttcataaaga cctccttatc gataagtttt
    26461 tcccaattaa aatcatatct caagaacgcc cgttattcac attttgttca taaatgatgt
    26521 tttttgtatc ataatcgtca aaaagaggtc gtgaccacgt cacaacctct tttt
//
LOCUS       DACCIQ010000033        25701 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCIQ010000033 DACCIQ010000000
VERSION     DACCIQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.033"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            133..3660
                     /locus_tag="IVM62_001385"
     CDS             133..3660
                     /locus_tag="IVM62_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6845352.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVM62_001386"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVM62_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP6845353.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVM62_001387"
     CDS             7448..7789
                     /locus_tag="IVM62_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845354.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVM62_001388"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVM62_001388"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP6845355.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVM62_001389"
     CDS             9146..11566
                     /locus_tag="IVM62_001389"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP6845356.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVM62_001390"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVM62_001390"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP6845357.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVM62_001391"
     CDS             13000..13737
                     /locus_tag="IVM62_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6845358.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVM62_001392"
     CDS             13751..14566
                     /locus_tag="IVM62_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6845359.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVM62_001393"
     CDS             14579..15226
                     /locus_tag="IVM62_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6845360.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVM62_001394"
     CDS             15242..15898
                     /locus_tag="IVM62_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6845361.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVM62_001395"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVM62_001395"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP6845362.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVM62_001396"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVM62_001396"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP6845363.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVM62_001397"
     CDS             18240..18758
                     /locus_tag="IVM62_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6845364.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVM62_001398"
     CDS             18865..19365
                     /locus_tag="IVM62_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6845365.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IVM62_001399"
     CDS             complement(19645..20565)
                     /locus_tag="IVM62_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP6845366.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IVM62_001400"
     CDS             20730..21743
                     /locus_tag="IVM62_001400"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP6845367.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IVM62_001401"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IVM62_001401"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP6845368.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IVM62_001402"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IVM62_001402"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6845369.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IVM62_001403"
     CDS             complement(23383..24144)
                     /locus_tag="IVM62_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6845370.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IVM62_001404"
     CDS             24333..25427
                     /locus_tag="IVM62_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6845371.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25701)
                     /locus_tag="IVM62_001405"
     CDS             complement(25513..>25701)
                     /locus_tag="IVM62_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6845372.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4455 c         5417 g         7320 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat t
//
LOCUS       DACCIQ010000034        24819 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCIQ010000034 DACCIQ010000000
VERSION     DACCIQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.034"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..213)
                     /locus_tag="IVM62_001406"
     CDS             complement(<1..213)
                     /locus_tag="IVM62_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845373.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIE"
     gene            complement(398..796)
                     /locus_tag="IVM62_001407"
     CDS             complement(398..796)
                     /locus_tag="IVM62_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP6845374.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(793..1566)
                     /locus_tag="IVM62_001408"
     CDS             complement(793..1566)
                     /locus_tag="IVM62_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6845375.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1563..2246)
                     /locus_tag="IVM62_001409"
     CDS             complement(1563..2246)
                     /locus_tag="IVM62_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6845376.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2413..3300)
                     /locus_tag="IVM62_001410"
     CDS             complement(2413..3300)
                     /locus_tag="IVM62_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6845377.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3297..4166)
                     /locus_tag="IVM62_001411"
     CDS             complement(3297..4166)
                     /locus_tag="IVM62_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6845378.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4303..5589)
                     /locus_tag="IVM62_001412"
     CDS             complement(4303..5589)
                     /locus_tag="IVM62_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6845379.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5862..6440
                     /locus_tag="IVM62_001413"
     CDS             5862..6440
                     /locus_tag="IVM62_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6845380.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6440..7369
                     /locus_tag="IVM62_001414"
     CDS             6440..7369
                     /locus_tag="IVM62_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845381.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7366..9102
                     /locus_tag="IVM62_001415"
     CDS             7366..9102
                     /locus_tag="IVM62_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6845382.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9165..10001)
                     /gene="pheA"
                     /locus_tag="IVM62_001416"
     CDS             complement(9165..10001)
                     /gene="pheA"
                     /locus_tag="IVM62_001416"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP6845383.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9998..10507)
                     /locus_tag="IVM62_001417"
     CDS             complement(9998..10507)
                     /locus_tag="IVM62_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP6845384.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10500..11795)
                     /gene="aroA"
                     /locus_tag="IVM62_001418"
     CDS             complement(10500..11795)
                     /gene="aroA"
                     /locus_tag="IVM62_001418"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6845385.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11808..12899)
                     /locus_tag="IVM62_001419"
     CDS             complement(11808..12899)
                     /locus_tag="IVM62_001419"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP6845386.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12916..14082)
                     /gene="aroC"
                     /locus_tag="IVM62_001420"
     CDS             complement(12916..14082)
                     /gene="aroC"
                     /locus_tag="IVM62_001420"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP6845387.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14084..15148)
                     /gene="aroB"
                     /locus_tag="IVM62_001421"
     CDS             complement(14084..15148)
                     /gene="aroB"
                     /locus_tag="IVM62_001421"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP6845388.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15181..16203)
                     /gene="aroF"
                     /locus_tag="IVM62_001422"
     CDS             complement(15181..16203)
                     /gene="aroF"
                     /locus_tag="IVM62_001422"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP6845389.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16862..17836)
                     /locus_tag="IVM62_001423"
     CDS             complement(16862..17836)
                     /locus_tag="IVM62_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP6845390.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17817..19700)
                     /locus_tag="IVM62_001424"
     CDS             complement(17817..19700)
                     /locus_tag="IVM62_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP6845391.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19718..21943)
                     /locus_tag="IVM62_001425"
     CDS             complement(19718..21943)
                     /locus_tag="IVM62_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6845392.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22058..23092
                     /locus_tag="IVM62_001426"
     CDS             22058..23092
                     /locus_tag="IVM62_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6845393.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23159..24172)
                     /locus_tag="IVM62_001427"
     CDS             complement(23159..24172)
                     /locus_tag="IVM62_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6845394.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24250..24438)
                     /locus_tag="IVM62_001428"
     CDS             complement(24250..24438)
                     /locus_tag="IVM62_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP6845395.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7139 a         5393 c         4427 g         7860 t
ORIGIN      
        1 ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca aaacagccga
       61 cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga actctggtga
      121 aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag atcgctcaat
      181 cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt catcattttt
      241 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      301 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      361 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      421 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      481 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      541 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      601 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      661 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      721 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      781 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      841 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      901 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      961 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
     1021 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
     1081 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1141 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1201 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1261 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1321 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1381 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1441 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1501 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1561 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1621 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1681 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1741 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1801 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1861 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1921 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1981 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     2041 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     2101 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2161 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2221 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2281 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2341 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2401 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2461 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2521 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2581 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2641 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2701 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2761 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2821 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2881 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2941 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     3001 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     3061 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3121 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3181 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3241 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3301 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3361 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3421 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3481 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3541 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3601 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3661 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3721 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3781 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3841 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3901 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3961 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     4021 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     4081 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4141 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4201 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4261 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4321 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4381 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4441 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4501 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4561 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4621 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4681 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4741 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4801 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4861 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4921 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4981 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     5041 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     5101 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5161 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5221 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5281 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5341 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5401 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5461 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5521 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5581 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5641 cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat ttctcctaaa
     5701 ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc tagaccaaag
     5761 atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc tagaaataag
     5821 tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata cgcgtaattc
     5881 ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat tggtctgagc
     5941 acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat ttttctttgg
     6001 tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat ggaatcgaat
     6061 tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc tctagctatg
     6121 atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat cttttaaagc
     6181 ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa
     6241 aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag aatctttcac
     6301 agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa cacaccttgt
     6361 ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta aacaaaaaca
     6421 gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc ctgtttctac
     6481 cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac ttaggaagtt
     6541 tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt ctgattcttt
     6601 tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca aaggacaacg
     6661 tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt tattaaccaa
     6721 ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact acgctttagc
     6781 tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg atccagctta
     6841 tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg ctgacgagca
     6901 tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat tagccgatgc
     6961 tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac tcacccactt
     7021 ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg agttcgtcca
     7081 ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc atttttcata
     7141 tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa aatatctaaa
     7201 aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta ctttgacgga
     7261 aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt ttaaagaaga
     7321 gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc
     7381 aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct ttttctgcaa
     7441 gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat ccagctgttc
     7501 aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag tttgaatgat
     7561 acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag ctatttttcc
     7621 aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca aaaaatgaat
     7681 gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt tggagaaaat
     7741 ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc tttttttcaa
     7801 tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct ttttgcacac
     7861 cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa aaaactgaaa
     7921 aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa gcaactagcg
     7981 aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat gaacgaagaa
     8041 cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat gattttcaaa
     8101 aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg tcttcctctg
     8161 taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc taaccaaatg
     8221 ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc tggctcattg
     8281 gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga ttcgctaaaa
     8341 cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca tgaagtccaa
     8401 ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta tgaacagacg
     8461 atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc acagatacag
     8521 ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca gaaaaaaaca
     8581 gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac gattgggaaa
     8641 aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta catcgatctt
     8701 ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga tgaactgcag
     8761 tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta tcttcatgct
     8821 tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa tcagcttctt
     8881 ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa acaaaaaaat
     8941 gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct aatgtatggc
     9001 gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt aacgatttgt
     9061 ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc caagcgctga
     9121 tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct acgcgtctct
     9181 tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag catcttgatt
     9241 tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc gatcacgaaa
     9301 aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa gttgatttcc
     9361 cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc aggtagtgtc
     9421 aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg gtcaccgacg
     9481 atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa gatctccaat
     9541 ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt cggatgttct
     9601 gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac attaggataa
     9661 tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgcgaaag gatttttgta
     9721 atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat ttcactttta
     9781 acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc tccttccaat
     9841 gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa
     9901 acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga
     9961 taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta
    10021 taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac
    10081 tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc
    10141 caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg
    10201 cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac
    10261 tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa
    10321 tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg
    10381 acaattggat atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt
    10441 gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc
    10501 tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa
    10561 gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct
    10621 acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga
    10681 ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc
    10741 tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg
    10801 tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca
    10861 tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg
    10921 acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc
    10981 atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac
    11041 aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata
    11101 tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt
    11161 ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg
    11221 gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa
    11281 gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg
    11341 gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc
    11401 atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg
    11461 aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta
    11521 ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca
    11581 tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt
    11641 aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca
    11701 attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc
    11761 cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag
    11821 caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt
    11881 ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta
    11941 atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca
    12001 tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc
    12061 aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca
    12121 gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat
    12181 aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc
    12241 ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg
    12301 aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt
    12361 cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa
    12421 aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa
    12481 taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc
    12541 attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc
    12601 ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca
    12661 tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct
    12721 gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca
    12781 cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata
    12841 caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc
    12901 ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt
    12961 caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc
    13021 tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac
    13081 acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt
    13141 aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc
    13201 gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc ataggatctc
    13261 atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc
    13321 tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca
    13381 ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc
    13441 gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc
    13501 tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat
    13561 atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat
    13621 gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc
    13681 tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc
    13741 taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc
    13801 aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac
    13861 taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg
    13921 atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc
    13981 gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt
    14041 taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt
    14101 aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca
    14161 agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt
    14221 catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta
    14281 aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta
    14341 ccattccaat agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa
    14401 ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat
    14461 cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt
    14521 ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta
    14581 tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt
    14641 ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt
    14701 tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta
    14761 gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg
    14821 ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat
    14881 tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg
    14941 ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt
    15001 ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa
    15061 tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt
    15121 tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa
    15181 ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat
    15241 gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc
    15301 aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg
    15361 tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt
    15421 taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta
    15481 agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc
    15541 tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt
    15601 acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg
    15661 taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat
    15721 cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt cttcttctaa
    15781 accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc
    15841 gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca
    15901 aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac
    15961 tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc
    16021 attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt
    16081 tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc
    16141 gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat
    16201 cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt
    16261 tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc
    16321 tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt
    16381 gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc
    16441 actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata
    16501 gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc
    16561 taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac
    16621 tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc atttttatga
    16681 aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca attggtttta
    16741 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
    16801 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
    16861 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
    16921 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
    16981 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
    17041 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
    17101 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
    17161 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
    17221 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
    17281 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
    17341 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
    17401 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
    17461 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
    17521 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
    17581 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
    17641 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
    17701 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
    17761 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
    17821 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
    17881 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
    17941 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
    18001 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
    18061 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
    18121 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
    18181 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
    18241 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
    18301 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
    18361 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
    18421 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
    18481 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
    18541 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
    18601 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
    18661 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
    18721 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
    18781 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
    18841 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
    18901 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
    18961 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
    19021 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
    19081 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
    19141 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
    19201 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
    19261 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
    19321 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
    19381 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
    19441 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
    19501 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
    19561 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
    19621 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
    19681 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
    19741 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
    19801 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
    19861 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
    19921 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
    19981 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
    20041 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
    20101 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
    20161 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
    20221 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
    20281 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
    20341 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
    20401 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
    20461 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
    20521 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
    20581 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
    20641 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
    20701 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
    20761 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
    20821 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
    20881 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
    20941 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
    21001 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
    21061 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
    21121 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
    21181 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
    21241 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
    21301 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
    21361 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
    21421 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
    21481 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
    21541 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
    21601 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
    21661 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
    21721 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
    21781 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
    21841 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
    21901 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
    21961 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
    22021 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
    22081 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
    22141 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
    22201 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
    22261 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
    22321 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
    22381 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
    22441 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
    22501 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
    22561 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
    22621 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
    22681 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
    22741 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
    22801 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
    22861 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
    22921 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
    22981 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
    23041 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
    23101 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
    23161 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
    23221 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
    23281 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
    23341 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
    23401 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
    23461 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
    23521 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
    23581 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
    23641 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
    23701 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
    23761 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
    23821 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
    23881 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
    23941 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
    24001 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
    24061 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
    24121 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
    24181 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
    24241 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
    24301 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
    24361 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
    24421 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
    24481 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
    24541 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
    24601 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
    24661 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
    24721 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
    24781 agtccttatt ggcaaatcgc caaaataatg tagactaac
//
LOCUS       DACCIQ010000035        24408 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCIQ010000035 DACCIQ010000000
VERSION     DACCIQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.035"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(70..615)
                     /locus_tag="IVM62_001429"
     CDS             complement(70..615)
                     /locus_tag="IVM62_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6845396.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(627..1319)
                     /locus_tag="IVM62_001430"
     CDS             complement(627..1319)
                     /locus_tag="IVM62_001430"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP6845397.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1338..1622)
                     /locus_tag="IVM62_001431"
     CDS             complement(1338..1622)
                     /locus_tag="IVM62_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845398.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1635..2198)
                     /locus_tag="IVM62_001432"
     CDS             complement(1635..2198)
                     /locus_tag="IVM62_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6845399.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2426..3088
                     /locus_tag="IVM62_001433"
     CDS             2426..3088
                     /locus_tag="IVM62_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP6845400.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3212..4408
                     /locus_tag="IVM62_001434"
     CDS             3212..4408
                     /locus_tag="IVM62_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP6845401.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4509..5885)
                     /locus_tag="IVM62_001435"
     CDS             complement(4509..5885)
                     /locus_tag="IVM62_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845402.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5922..6788)
                     /locus_tag="IVM62_001436"
     CDS             complement(5922..6788)
                     /locus_tag="IVM62_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP6845403.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6877..7902)
                     /locus_tag="IVM62_001437"
     CDS             complement(6877..7902)
                     /locus_tag="IVM62_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6845404.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7920..8594)
                     /locus_tag="IVM62_001438"
     CDS             complement(7920..8594)
                     /locus_tag="IVM62_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6845405.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8778..9671)
                     /locus_tag="IVM62_001439"
     CDS             complement(8778..9671)
                     /locus_tag="IVM62_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6845406.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9745..11442)
                     /locus_tag="IVM62_001440"
     CDS             complement(9745..11442)
                     /locus_tag="IVM62_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6845407.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11435..12748)
                     /locus_tag="IVM62_001441"
     CDS             complement(11435..12748)
                     /locus_tag="IVM62_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6845408.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12748..16785)
                     /locus_tag="IVM62_001442"
     CDS             complement(12748..16785)
                     /locus_tag="IVM62_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6845409.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(17554..18072)
                     /locus_tag="IVM62_001443"
     CDS             complement(17554..18072)
                     /locus_tag="IVM62_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6845410.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18074..18691)
                     /locus_tag="IVM62_001444"
     CDS             complement(18074..18691)
                     /locus_tag="IVM62_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6845411.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18688..19542)
                     /locus_tag="IVM62_001445"
     CDS             complement(18688..19542)
                     /locus_tag="IVM62_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6845412.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19539..20450)
                     /locus_tag="IVM62_001446"
     CDS             complement(19539..20450)
                     /locus_tag="IVM62_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6845413.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20491..21825)
                     /locus_tag="IVM62_001447"
     CDS             complement(20491..21825)
                     /locus_tag="IVM62_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6845414.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22033..22863
                     /locus_tag="IVM62_001448"
     CDS             22033..22863
                     /locus_tag="IVM62_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP6845415.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23107..23356)
                     /locus_tag="IVM62_001449"
                     /pseudo
     CDS             complement(23107..23356)
                     /locus_tag="IVM62_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23547..23741)
                     /locus_tag="IVM62_001450"
     CDS             complement(23547..23741)
                     /locus_tag="IVM62_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845416.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7182 a         5300 c         4219 g         7707 t
ORIGIN      
        1 aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac
       61 tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc
      121 atgtccaacg ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag
      181 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
      241 aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga
      301 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
      361 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
      421 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
      481 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
      541 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
      601 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
      661 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
      721 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
      781 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
      841 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
      901 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
      961 tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct
     1021 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     1081 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     1141 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     1201 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     1261 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     1321 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     1381 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     1441 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     1501 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     1561 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     1621 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     1681 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     1741 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     1801 ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa
     1861 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     1921 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     1981 actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa
     2041 gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     2101 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     2161 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     2221 atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca
     2281 tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc
     2341 tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa
     2401 cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt
     2461 ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt
     2521 gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca
     2581 agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac
     2641 agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca
     2701 attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc
     2761 agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga
     2821 agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa
     2881 tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc
     2941 gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga
     3001 ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa
     3061 taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat
     3121 tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat
     3181 cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga
     3241 tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac
     3301 agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc
     3361 tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc
     3421 aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa
     3481 attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt
     3541 aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg
     3601 tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga
     3661 agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga
     3721 taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa
     3781 caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga
     3841 acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat
     3901 ggatgttcag attgccaatg attacacgca attcctagat cgtctggata aacgtacgca
     3961 tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca
     4021 aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct
     4081 gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc
     4141 tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa
     4201 aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct
     4261 tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga
     4321 aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa
     4381 acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc
     4441 caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc
     4501 attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc
     4561 agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc
     4621 aatacaatac aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat
     4681 tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc
     4741 atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat
     4801 ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc
     4861 ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt
     4921 aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct
     4981 acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg
     5041 tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg
     5101 taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg
     5161 tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca
     5221 agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca
     5281 ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat
     5341 accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg
     5401 aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg
     5461 ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca
     5521 gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct
     5581 gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc
     5641 actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca
     5701 gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc
     5761 accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt
     5821 tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa
     5881 tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac
     5941 cctaaaagga taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct
     6001 tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc
     6061 atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag
     6121 atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact
     6181 ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag
     6241 ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca
     6301 atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta
     6361 tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct
     6421 aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct
     6481 cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca
     6541 cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat
     6601 aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact
     6661 aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca
     6721 attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa
     6781 atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta
     6841 acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt
     6901 taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa
     6961 atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg
     7021 tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc
     7081 ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt
     7141 ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa
     7201 ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac
     7261 gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg
     7321 aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct
     7381 tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa
     7441 tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga
     7501 aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt
     7561 actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc
     7621 taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt
     7681 ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata
     7741 aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt
     7801 gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa
     7861 gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt
     7921 cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt
     7981 tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt
     8041 tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc
     8101 atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt
     8161 tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat
     8221 aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg
     8281 atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc
     8341 atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt
     8401 tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt
     8461 tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat
     8521 tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc
     8581 atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag
     8641 cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac
     8701 tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat
     8761 tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt
     8821 cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt
     8881 aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag
     8941 ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg
     9001 ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg
     9061 acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat
     9121 cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta
     9181 tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg
     9241 actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca
     9301 ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga
     9361 ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt
     9421 actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt
     9481 ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa
     9541 taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac
     9601 atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt
     9661 ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg
     9721 attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca
     9781 gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc
     9841 accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc
     9901 tactcctacg atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa
     9961 ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata
    10021 ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt
    10081 tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg
    10141 cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct
    10201 tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt
    10261 tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat
    10321 ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa
    10381 attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac
    10441 tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc
    10501 tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg
    10561 atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc
    10621 gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga
    10681 agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt
    10741 aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct
    10801 gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc
    10861 atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga
    10921 cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct
    10981 agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt
    11041 cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg
    11101 tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa
    11161 aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac
    11221 tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc
    11281 cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc
    11341 gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag
    11401 taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt
    11461 tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc
    11521 ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac
    11581 cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg
    11641 ttgcggattc ataggcagag actggaactt tcccggattc taattcatcc atctcctgtc
    11701 cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga
    11761 cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa
    11821 aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg
    11881 aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt
    11941 gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct
    12001 gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac
    12061 gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa
    12121 tctcttgcag ttcctcgctt gattctgtcg ggtgataaat tggcaaaata gcagcaattg
    12181 ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga
    12241 aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc
    12301 cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa
    12361 gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga
    12421 cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt
    12481 cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt
    12541 cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat
    12601 aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt
    12661 cttctgccga catcgcctgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt
    12721 gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac
    12781 aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa
    12841 taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag
    12901 ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg
    12961 cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt
    13021 tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat
    13081 aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac
    13141 cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc
    13201 tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc
    13261 ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag
    13321 ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg
    13381 agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc
    13441 aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc
    13501 atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac
    13561 tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc
    13621 taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat
    13681 atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc
    13741 ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt
    13801 tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat
    13861 agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc
    13921 tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt
    13981 ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac
    14041 atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc
    14101 gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt
    14161 ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt
    14221 gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc
    14281 atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc
    14341 ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc
    14401 atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg
    14461 gaattgatac gtctcatata ctaactgata acgtggtggt ccagcgatct ctctgatcca
    14521 aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat
    14581 cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg
    14641 attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt
    14701 gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat
    14761 ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt
    14821 cactgtgtaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct
    14881 gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc
    14941 gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc
    15001 ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg
    15061 aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag
    15121 tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat
    15181 gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc
    15241 aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc
    15301 atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag
    15361 ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc
    15421 attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc
    15481 ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga
    15541 tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa
    15601 atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct
    15661 ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg
    15721 tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc
    15781 tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg
    15841 tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg
    15901 aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt
    15961 caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt
    16021 tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact
    16081 catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc
    16141 gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc
    16201 agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata
    16261 atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg
    16321 aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg
    16381 ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg
    16441 cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt
    16501 atagtcattc gttgtaaatt tcgggtagcg atgctgttcg ttttcaactt ctccgctagg
    16561 atagatatat tcataggggt cttgcgtagc tgttggccca agacgctcca aaataccgtt
    16621 gaataactgg cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg gttcgtccag
    16681 cttttccatc tcattttctt cgctacgttg aatttcagcg gaatcttctg tatcagtttc
    16741 attttcttgt aattcttggt tatctattct ttcatcaacc accacttgaa gaggtagttc
    16801 cccttcgttc acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc
    16861 ttttgaataa acttttccga cataccattc ttgaccatcg atcgtctcga cttttaggtc
    16921 ttctccatct tcatcatagg aggatagacc tgttccacga acgtttttta cggtaccaat
    16981 cccggcggct gccgcatcga ttgctaaacg tattctacct tcattggcac tttccttttt
    17041 ttcaaaagcc agatgccatt caagggtttc ttctttttca atatacttcc cttcaatttt
    17101 tgcatagtcc tcatctaaga atacctcatc ctcctttgtt tctgttgact cagcagctac
    17161 atccagggtc gtcatatgat agactgactg gaaatatggt ccaaatagca aactgacagt
    17221 tactaactgc aaaagcttca attttaaccc tttcacatcc ttatactcct ttcaaaaata
    17281 tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc taattataat ttaatttaat
    17341 tttaatttta aatcaaatga tatccttttt agttaaaaaa caccaattat acaacattta
    17401 ttattaaaat taaattataa aattttataa cgttctcatt taacaaaact taagttatta
    17461 agacaaatga ataaacaact acgctttcct cgaaacaaaa aaataaagca caaatgcgca
    17521 tttacgcact catgctttat tcgtacaatt aattcagata acggcagcgc ataggatgcc
    17581 aataacactt cctaaaatag ctcctcctaa aatgtcagta ggataatgta aaagtaaaaa
    17641 aatcctcgaa taagcgatca aacaagcaaa taaaaaggct aatattcctg caactggact
    17701 taattcgaac aacgcagcta ctgataaaaa ggctagcgct gtatgtcctg aaggaaaaga
    17761 agaagtcttc ggtttacttg tgtaaaggac aagccgacga tttttttcat aaggtctttt
    17821 ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa aaatagatca aactgccgat
    17881 aagaatggct agaccatatt ctctagtatc aggtatcaag actaaaacag cagcaaggag
    17941 taaccaaata aaaccaccat tagagatatt actgataaat ttcaaaaaat tgatttttcg
    18001 actatccgtt ttccgttgct tggcgaactt gttgataata ctatcatcga ttttagcaat
    18061 caaacgcaac atgttattcc tcctttttaa gaaccttttc ttgcgtacga tggataaaaa
    18121 gcaggacttg aaaataaaac aagaaactga aacctaataa agcaaaatag attggtcctg
    18181 caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa atagaaaagc gcgcttttga
    18241 aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc ttctttttgt tccagttgaa
    18301 gcaagatttt gtaactaaag agaagcgcgc ttaatagaag caaaaacaac gtagtataca
    18361 tcaatgtata gccaattccc ccaccccaat agcggaaaat ctgataatcg atcaatagaa
    18421 gaagaaaata aacacttgct aaactatctt gctttccata aagagagaag ttctcacgaa
    18481 actttttcca gaatagtgaa ccgagctttt tctcttcatt ttttttgtaa tgataaatca
    18541 tatacagaac tgttcgactt gcagttccta atgtgaaaac aggcaagacg cacaggaacc
    18601 atagtagctg taaatggata agacgtggaa tccattcaat gatttctaag aaataatcat
    18661 tggctttttt accaatcgat cgattcatca tccttttgaa cctcctgtca aatccatccc
    18721 ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc gtcgggatca agacaagtgt
    18781 tgctcctgcc atcagataat tccattgtgt cgttgcttgg ttctgaaaaa cttgcaagcc
    18841 aatctgtaac gtgtattttt cttgatcttg gatatataac agaggaccta agaaatcatt
    18901 ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag gcaggtttcg tcaatggaag
    18961 catcaaatga ctccaaatat acaaatgatt cgctccgtcg atttcagcag cttcgatcaa
    19021 ttcattattg atcgataagt aaaattgacg catcagaaat atattgaacg cattgccgaa
    19081 gaacgatgga acgatcaggg gcaaataagt acctacccag ccgatcttgg agaataatac
    19141 atattgaggg atcatcgtta caaaccccgg gatcatcatc gtagaaagaa ccaaagcaaa
    19201 catcagcttt ttcccaggaa aatcaagctt cgcaaatcca taagcaataa aagaatttga
    19261 aaggacatta ccgatcacta ctagcacagt gatgaataat gtatttcttg catagcgcca
    19321 aaaaggaaag gcggcaatcg tatccagata gttttcccat acgatttttt ctgggaaaaa
    19381 ggtcggagga taacgaacga tttcttccat tggtttcaat gaagtaaaca ccatccaaag
    19441 caatggaatg atcaaaatga ttcctattac tgtcatgagg acataactta gtattttttt
    19501 tgtttgccga ttcacactgg tctttttgaa ataatccatc atttgccgtc acctccagaa
    19561 taatagaccc acttaggtgc aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga
    19621 aacaggatca ttgacatagc catcgcataa cccatatcaa aagctacgaa cgccttgttc
    19681 cacatatgca gattataaaa aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc
    19741 atgacataag cttcttgaaa aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa
    19801 aaaataatag gagtgattaa tggcaaagta atacttttga actttcccca aacgcctgct
    19861 ccatcgattt ctgctgattc atataaagat ctaggaacat tttgtaatgt agctaagtaa
    19921 agtagcattg ctcctccaga actccatatt ttcatcaaaa tcaacgcagg tttcgtccat
    19981 tgtggatcaa acagccaagc tggtccaggg atatggaacc agcccagaaa tgtgttgatc
    20041 aatcctgcag atggagaaag cagctgcatc cataagatgt agactgctac tcctgataag
    20101 actgccggta aatagaaagc tgtacggaat acactgattc ctttgatttt ttgattgagt
    20161 actgtggcta aaaacacact gaaaatcgtc gtcagcggta cattgaagat gacgaaataa
    20221 acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt
    20281 gctagaccaa tgaaattcat cttggatgtc aaattataat cggtgaaact accaataact
    20341 gaaaaaagca taggccctag cataaatacc aagaacccga taataaaagg cgagataaat
    20401 aaataggccc atcttgcttc ttttttcttt cggatagata aacgcttcaa attttcttgc
    20461 ctcctctgta cctagacagt caaaaatgga ttattgattt tgcttgacta agttttctac
    20521 tgctttttgt gcatctgcca atccttcttt tggtgatttt gttcctaaaa gaatctggtc
    20581 aatctgttca ttcactaatg tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac
    20641 cgtattttta aagttttcgt ccgccatttg atagatcatt tttcctttat ccgttaggtt
    20701 gtcattattc actaaattct cattggctgt cttgctcgcc atgatatcaa aacttttttc
    20761 gccgaatttt tcttgtactt ctggagaaga aagatatttg atgaattcat aagaggcctc
    20821 aggatctttt gctccgtatg gtacttccaa gacgaatccg ccaccccacg accaatggcc
    20881 tgaaccttct tctttttctg ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt
    20941 ttccatcaaa ctagagtaat aattgatatt ttgggcacgc atggctacta atccggaaat
    21001 aaatggatca gatacaccag acccaaattc ggcttctaat ttattgatcg tatttttccc
    21061 ataataatct tgccagtctt ggatccactc caaggcttcc actttatttt tagtatcaat
    21121 cttgactttt tcatctttgt caaaccaact agtcccgtta tctgcgttta gcgcccatac
    21181 atcagcccct aaattccata atggataaaa accgatgcgt gtaaattctt ttccttcttt
    21241 tttatctagt ttacgtgctg tttcttctaa ttcatcccaa gttgtcggta gatcttgttc
    21301 actgattcca gcttctttaa ataacttttt attataaaaa atgacttgtg tatatgtatt
    21361 gaatgggatc gcataaggat caccttcata ttctactgta tcccaaagct gcggataaaa
    21421 atccttagaa gtgtcttttt ctacatattt ggagagatta gttgcttgct gtgcttctgc
    21481 tctttgggca acggaattta tgtcctgtac aataacatcg ggtgcatttc cagctgttat
    21541 tgcagataaa gatttagtcc agatgtcacc ccatggctga tacttataat ttacatggat
    21601 ctcgttttgt gattgattga aatcctcaat gatttcttcg atagcttctt ttcgtgcacc
    21661 ggatccccaa aaggaccaga aatcaatatt ttttcttcca tcacttgttt tttcagaaga
    21721 atttgctgca cttgaactgg catcgcttgc attacttgat ccgcatcctc ctaataaaaa
    21781 caatgctgaa cttgccagca accctgtaaa caataatttt ttcatatttt acctccaaat
    21841 tttaatgtaa ccgatgtcgg ttttaatata acgatatcat tttgttctga aaataacaat
    21901 ttataaggta tgattctaaa aaaattatta atgataccaa tactttatat aattcgcaag
    21961 cgttttcttt gaatttttta atctttcgta gcagaatgag aatgtattaa aaatagatta
    22021 ggaggaattt tgatgtttag acaccagaaa gaattgcagt ttgaagcaaa accagatcgt
    22081 ccgaatccat taatcgcgaa atatctgcag gagctgatcg gcggccaata tggcgagatg
    22141 agtgtagcta tgcagtacct gttccaaggc tggagctgcc gaggagaaga aaaatataaa
    22201 gatatgctga tggacattgc aacagaagaa atcggccacg tggaaatgtt ggcgacaatg
    22261 gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat
    22321 ccaacaatcg gtgccattat ctctggaatg aatccacagc acgcgatcgt agctggctta
    22381 ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg gtgcctatat cattgcgagt
    22441 ggcaacttgt tagcagactt tagagctaat ttaaatgccg aatcacaagg aagacttcaa
    22501 gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa aagatatgct gtctgttctc
    22561 cttgcaagag atagctatca tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa
    22621 aaagaaggtt caatcttagt cccaagtact ttcccgcgtg acaaagaacg aaaagacatt
    22681 gcttatgact tctataactt ctcagaggga gaccaaagta gtgaaggttc ttgggctagt
    22741 ggcaaagcac tggatggcga aggcgaatat caatggttga aagaacctaa gatcgaaggc
    22801 gctgcaccaa aattagaacc agtaacacca aaaatgtatg ggacacctcc attcaagaaa
    22861 taaatatcta cccgtagaaa cacccgtttt ttgttccaca cgctttgtga gcaaaactga
    22921 ctaaagtcat taatcataac cacccttcaa aagggtggtt ttctctagag ctataagctc
    22981 aaatcaccgg ccagcgccta aagacgctgg ctttcatatt ttatgttcaa gccatattgc
    23041 ctttgccagt tttgcccacc tcttaaaagg tttatgtact actccttagc ccttaaaagg
    23101 atcgtcttac tctcgtacac tcagcttatc taacgcacta tcatgttttt cttgttcttg
    23161 aatgtacttt ttgatggtcg cttcattttg tcctactttt gtcacatagt atccttccac
    23221 ccaaaagtgt cgatacccaa atttatattt tagatttgca caatctcttg atgatctcct
    23281 gaatattggc tcgatattga ttataaatta tttttcttct atactttggt gtgaatacaa
    23341 tgtggtattt acacatctac tttgtacggg ctaaactatt tccctttatt gctccacccg
    23401 catagcaagt ggttttgtat ttcaatcgct cggcgcgctc aattaggtct aaagacaata
    23461 aatctaaaaa cccgctgctt cactgacaga aacaacgggt tttcagaaaa aaatgaaatg
    23521 aaaaagatct cttacggcaa tctattttat ttttttgatt ttttataaat ggaaaatgct
    23581 tcaagaagtc caagaaataa agccgtataa aaaccagaac ctatgaaatc tttatttatt
    23641 agatattcaa cagaaatccc gataacacta gccaacaata tatttatcag aagtgtaaaa
    23701 taaggatgct ttttatagaa tagaactagt gttgttttca tagactctcc tttcttcata
    23761 tttccaatta atcacaaatt cacggcatta ctagaaaaat taaaggggcg cagaagtgga
    23821 aaaatactct tcgactttcc ctacaaacca ggccatcttg tgtttaataa ctataccaaa
    23881 tctaaggtac ctctaaatct aaattccatt tgtggcggtg acctccaatt tttaactctt
    23941 ctatctccca tccagtatac ataaccttga tacttcgacc acttatatat cgaacacaca
    24001 aatacctatc cattgcagga gtactaccta acttagttgc tacttgcatc taaataacag
    24061 ctgaaaagat aggacttact cttagtaaag tatctgaact attaattttt aaactatcat
    24121 ttttatcctc aactacatgc acatctgttg caacttcact ttctgtctca tccagataaa
    24181 tgattaagga atcgggaatt gcaatatctt cagctgacgc tggaatagtt gagctaaatt
    24241 agtcatcata tctttattat aaaataacca acagcaaaaa atacaatgaa aaacaaactt
    24301 ggaactaatg tatcattaat aaatgttgaa aaattcagca actcattact gtatgatttt
    24361 agcagcccaa ccaacatata ccctataaaa gctaataaga tggttagg
//
LOCUS       DACCIQ010000036        23978 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCIQ010000036 DACCIQ010000000
VERSION     DACCIQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.036"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(45..779)
                     /locus_tag="IVM62_001451"
     CDS             complement(45..779)
                     /locus_tag="IVM62_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6845417.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IVM62_001452"
     CDS             1106..1516
                     /locus_tag="IVM62_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP6845418.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IVM62_001453"
     CDS             1509..2210
                     /locus_tag="IVM62_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6845419.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IVM62_001454"
     CDS             2210..3796
                     /locus_tag="IVM62_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6845420.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IVM62_001455"
     CDS             3815..4036
                     /locus_tag="IVM62_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP6845421.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IVM62_001456"
     CDS             4033..4794
                     /locus_tag="IVM62_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP6845422.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IVM62_001457"
     CDS             5174..5683
                     /locus_tag="IVM62_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6845423.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IVM62_001458"
     CDS             complement(5758..6297)
                     /locus_tag="IVM62_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP6845424.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IVM62_001459"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IVM62_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP6845425.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IVM62_001460"
     CDS             7364..8161
                     /locus_tag="IVM62_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP6845426.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IVM62_001461"
     CDS             8189..8824
                     /locus_tag="IVM62_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP6845427.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IVM62_001462"
     CDS             8844..9617
                     /locus_tag="IVM62_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6845428.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IVM62_001463"
     CDS             10025..10822
                     /locus_tag="IVM62_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP6845429.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IVM62_001464"
     CDS             10800..11213
                     /locus_tag="IVM62_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845430.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IVM62_001465"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IVM62_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6845431.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IVM62_001466"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IVM62_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP6845432.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IVM62_001467"
     CDS             15990..16550
                     /locus_tag="IVM62_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP6845433.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IVM62_001468"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IVM62_001468"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP6845434.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IVM62_001469"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IVM62_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP6845435.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IVM62_001470"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IVM62_001470"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP6845436.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IVM62_001471"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IVM62_001471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP6845437.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IVM62_001472"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IVM62_001472"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP6845438.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IVM62_001473"
     CDS             21897..22475
                     /locus_tag="IVM62_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6845439.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IVM62_001474"
     CDS             22527..23009
                     /locus_tag="IVM62_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP6845440.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IVM62_001475"
     CDS             23126..23500
                     /locus_tag="IVM62_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845441.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7840 a         4309 c         5142 g         6687 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aagtcccttc
    23881 tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta tgaatgattt
    23941 tactacagaa attgtgcaaa ctctagtcac taaaggcg
//
LOCUS       DACCIQ010000037        23644 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCIQ010000037 DACCIQ010000000
VERSION     DACCIQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.037"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IVM62_001476"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IVM62_001476"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP6845442.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IVM62_001477"
     CDS             complement(1173..1868)
                     /locus_tag="IVM62_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP6845443.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IVM62_001478"
     CDS             complement(1881..2597)
                     /locus_tag="IVM62_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP6845444.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="IVM62_001479"
     CDS             2927..3535
                     /locus_tag="IVM62_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP6845445.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3860..4183
                     /locus_tag="IVM62_001480"
     CDS             3860..4183
                     /locus_tag="IVM62_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6845446.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4365..5735
                     /locus_tag="IVM62_001481"
     CDS             4365..5735
                     /locus_tag="IVM62_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP6845447.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5865..7166)
                     /locus_tag="IVM62_001482"
     CDS             complement(5865..7166)
                     /locus_tag="IVM62_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6845448.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7520..9511)
                     /locus_tag="IVM62_001483"
     CDS             complement(7520..9511)
                     /locus_tag="IVM62_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP6845449.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9655..9921)
                     /locus_tag="IVM62_001484"
     CDS             complement(9655..9921)
                     /locus_tag="IVM62_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6845450.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="IVM62_001485"
     CDS             complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="IVM62_001485"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP6845451.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="IVM62_001486"
     CDS             complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="IVM62_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP6845452.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13265..13828
                     /locus_tag="IVM62_001487"
     CDS             13265..13828
                     /locus_tag="IVM62_001487"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6845453.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14081..14602)
                     /locus_tag="IVM62_001488"
     CDS             complement(14081..14602)
                     /locus_tag="IVM62_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP6845454.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14605..15447)
                     /locus_tag="IVM62_001489"
     CDS             complement(14605..15447)
                     /locus_tag="IVM62_001489"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP6845455.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15440..16165)
                     /locus_tag="IVM62_001490"
     CDS             complement(15440..16165)
                     /locus_tag="IVM62_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP6845456.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16341..16637
                     /locus_tag="IVM62_001491"
     CDS             16341..16637
                     /locus_tag="IVM62_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845457.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17205..18797
                     /locus_tag="IVM62_001492"
     CDS             17205..18797
                     /locus_tag="IVM62_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6845458.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18810..19550
                     /locus_tag="IVM62_001493"
     CDS             18810..19550
                     /locus_tag="IVM62_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6845459.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19718..21067
                     /locus_tag="IVM62_001494"
     CDS             19718..21067
                     /locus_tag="IVM62_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845460.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21195..21785)
                     /locus_tag="IVM62_001495"
     CDS             complement(21195..21785)
                     /locus_tag="IVM62_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6845461.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21932..23284
                     /gene="brnQ"
                     /locus_tag="IVM62_001496"
     CDS             21932..23284
                     /gene="brnQ"
                     /locus_tag="IVM62_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6845462.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4768 c         4506 g         7600 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcaaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc ttttttattt
     3601 ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca aacgaaaaat
     3661 cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa atttatactt
     3721 tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga atgaaccctc
     3781 gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa cattcgttga
     3841 ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc gttacgcttc
     3901 agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt ttattattga
     3961 cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt tgatcgaaaa
     4021 tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa tggatattga
     4081 tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg atgacggtac
     4141 gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata gataaacgtg
     4201 gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa ttcttggctt
     4261 ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca ggataaacgc
     4321 tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc gatttaacaa
     4381 aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc ggaaatgttt
     4441 ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta ggtaaaaatg
     4501 cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc tttgcccaag
     4561 gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa gattatcggg
     4621 gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact gtgattttaa
     4681 ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact cctcctgaaa
     4741 tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt gcatcagtca
     4801 gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca ccactattct
     4861 ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt atctattttg
     4921 gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct tccagcgtac
     4981 tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa aaagatttca
     5041 aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg gcgtttcaat
     5101 cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt ctagggacag
     5161 atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat cagccgatga
     5221 tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg aaagaatatg
     5281 gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt agtctcttag
     5341 caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt agcccatcag
     5401 aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct ctttactgga
     5461 tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa agcaagatcc
     5521 ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc gttcttacag
     5581 gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc ggttctgttg
     5641 ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg ggtgaagagg
     5701 aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa atcgttgcgg
     5761 ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca ggttttttag
     5821 ataacaaaaa ggctgtgaca aaaaatgtca cagccttttt tcttttattc tgtttttctt
     5881 tccgtggact ctactttgaa ataatcgata tgtgcatgct ggtattctaa taccgtgaaa
     5941 cggaatgatg caatctcaat gattgttcct gcttttattt ctttatatcg gctagaagca
     6001 aagcccgcaa gtgtagtaaa attcgtttct tcaaactctg gaatctctac atgaaaataa
     6061 cgctggaaat catctaatgt tagtttgccg gagatgatat aagagccatc tgactgttcc
     6121 gctatcaatg cttcgtccaa atctgtctca tctcggattt ccccgacgat ttcttctaaa
     6181 atatcttcaa tcgtcactaa gccgcttgtc cctccgtatt catcttttaa gatagcaata
     6241 tgcttaccct tttttcgcat gatcgtcaat aattttttta tagggatcgt ttcagttgtt
     6301 acgatcggct cttcaatcaa ctgttctact gtcagctggt catctttata agattgcttg
     6361 atcaaatctg gcaaggtgac ataacctaaa acattgtctt ttgattctaa gataaccgga
     6421 tagcgagtat gtccttgttc aatcgaagct tttatcgctt ctttgacggt ttctgaagct
     6481 tcgaatactt gcatggacgt acggtctact tgtatatctt ttgccaaggt atcatcaaat
     6541 tcaaacacgt tttccaaata ataatattct tctttggtca actctccttt ttcataagaa
     6601 ttcaatgcga ctaatgtcag ctcttcttga gacaatgtct cttctccttc agatacgaga
     6661 cgcacaccta atagcttccc tagccctgca gctgaatgat tcaataccca aataaaagga
     6721 aacattactt tataaaaata atgaagtggt ttaactacga acaagacgac cttctccgta
     6781 ttgacgatac tatagctctt aggaagcagt tcgccaataa ccacatcgat atacgtaact
     6841 aaaataaatc ccagcgccac agagattgta ctgctaatag ccgaactaaa aggtagttta
     6901 ccgatcactg gttctagtaa agccgcgaca gatccttcac cgacccaccc gatgatcaaa
     6961 ccggttagcg taattcctaa ttgactagct gataaatagt tatctaaatg gtgtgtaaca
     7021 tgaagagcta gcttggcgtt ctttacattt tccttttcta gttgttccaa tcttgatcgt
     7081 ctgattttca ctaaagcaaa ttcacttgct acaaaaaatg aagtaattgc aatcatcaaa
     7141 aataaaataa tgaaattgat aaccatattc tcccgccttt ctctttttct attgtaccct
     7201 ttttctacaa aaagattgaa aataatgcgt attcatattt aggaataaaa aaggagatgc
     7261 ggcatttttc agccacatct ccttatcact tcaaaatcat tgcgttactt gttgctaaac
     7321 agctttttca tgacctctga taaaaacggt attcaaaagc ttgcatctac ggcattaagc
     7381 ccaaacagat gaaaaacatg agaagctgtt tttatctgct cgttcttact gcttgttgca
     7441 gtacagcttt ttcatgacct ctgataaacg gtgttcaagt agctgacatt cgacattagt
     7501 tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca tgttttccga gtatttccaa
     7561 tgataagcgg aacagtttca tgttttactt cgctgtactc tagcccaaac catgtttctg
     7621 gcgaggtagc gatttttttg attgccagtt ttgcctgcat gatttgacgt tgccattttc
     7681 ctagagttgt cacattggaa actacttcat caatcaggac aaattggaaa tcgccaactt
     7741 ctcgccctgg tgtcagagag tatctttgtg gctgttttgg caaacggcct tgtttcatca
     7801 aatcatggat gacttggcga acatatacgt tgatttcctg ttggaccctg aaacctaaga
     7861 acagattcac ttggacaata aaatcagtgt ccatcatatc tacttgatat tctttcgtat
     7921 atggctcatc cgtcacttcc acatgaacaa accaatagac tcgtgctctt ttaggccgtt
     7981 tatctaaaat cgaatagatg aactgtcggc caactttatc ttcagccata tcaggaacca
     8041 agaagaccac atttgtctga tagattggta aagaagtatc atcacgtaat tctctaagct
     8101 gcgggatata atctctcaaa gacacatcct cggttgacga ttcgcgaata atgttccctt
     8161 gttcccaaat aaacatcacg actaagatga tcaaaccaat cagaacggct acaaaaccgc
     8221 ctcggaagaa tttgacaaca cttgagataa agaaaacgac ttctatcgca ccgaagaaaa
     8281 aggtaactaa atatgcaagc caacgagaat gcccattttg gataagataa aagtaaagca
     8341 gtgctgttgt catcaacatc gtcacagtga tcgatagacc gtaagcagct tccatatgat
     8401 gagacgttcg gaatgtgaca accactagag aacaggcgat ccataaaatc agattgactg
     8461 ctgggatata catttgcccg atactgttcc caggatacat gatctttaac cgaggcagca
     8521 gtttcaactt gattgcttct gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag
     8581 caatgactgc agcgactgtt gcaaaagcaa cgccgatcac gatccaactt tgaggcatca
     8641 tttggaagaa tggattcagg ttcttgattt gctgtgcttc agggttttga taaacattca
     8701 acaaccacgc agcctgccca aaataattga taaccaaaca aattttgacg taaggccagc
     8761 tcagacgaat atttcgtttg ccgacatgtc caagatcgga ataaagcgcc tctgcacctg
     8821 tcgtagccag gaaaatattt ccaagaatga acaagcctaa tttgttatct gcactaaaaa
     8881 gcagctggat agcataataa ggattcagtg cacggataac tgtccagtct tgactgaagt
     8941 tcattaatcc cattacgcct aaaaaagtaa accaagcgaa catgattggt ccgaatgctt
     9001 ttccgacaat atctgtacca aagcgttgga ccatgaataa aacaaaaata ataactaaag
     9061 tgataatgac gatcacgttc tggttattac cgaaccggtc aaaaaactgc ggaattcctc
     9121 gcaagccttc aatcgcagtc gttaccgtca cagcaggggt caatacccca tctgctagta
     9181 aggcagctcc tccgatcatt gcaggaatga tcaaatattt cccacctttt cggacaagtg
     9241 tgtagagtga gaaaattcca ccttcaccat ggttatctgc attcaatgca atcacgacat
     9301 atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa
     9361 aatcaggatt gattcctgac aatccgccat tatcttcaac aatcgatttc ataacgtaaa
     9421 gcggacttgt cccgatgtct ccgtagacga ctcccatcgc tacaagcagg ccagccattg
     9481 atatcttttt ggtattgtgc gttgttgcca cgtttttccc tcatttcttg ttatatggat
     9541 atcctttagg taagcttgaa tgatagatga tattcttatc catgacaccc gctttcctaa
     9601 attgcttcta attatccaag taacacactg aagtgtcaac ttatttgaat aaaattattc
     9661 tttctttgga cgttctctga tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat
     9721 tgcacccaat aatgctggta tcaaataaaa accgccgagt tccggtccca gtggtcctag
     9781 taaaaattcg ccgagccaac tacctaaaaa accgataatg atatttccag atacgcctcc
     9841 cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct aatataccgc cgataaataa
     9901 cgaccataaa aaattcagca tctcctttcc ctcctttatc catatttctc acttttattt
     9961 tacgctttat tcagaaggac tccaaaaata tgcgccaaga aactccagaa aaaaagaagt
    10021 tgtaacaaaa gtgttcagct tatttccgta gaagctgctt ctggaacacc gtgaatcaga
    10081 ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    10141 atattttgcc gttttttgag ctaatatcga gggacagctg attgaacatc gtttattgga
    10201 gaggttgtaa aaaagctgtt ctgcatcaag taataagaac agccaaataa aaacagctcc
    10261 taatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10321 tcgaaaaaaa aggactgtga cacggctttt gtcacagtcc ccttttttcg aataattatt
    10381 gtccccctgt caccttcacg atcacaagta cattattcct atgaattaaa ttatttttct
    10441 tcatttttcg ttgaaccatc ttcgttcaac attgcttttc ttgacagatt tacgcgtcct
    10501 tgtttatcga tttcagttac tttcacaagg atctcatcac ctagtttaac aacatcttcg
    10561 actttattga cacgatcgtt tgctaattgg gaaatatgga caagtccatc ttttcctttg
    10621 atcaagttga cgaatgcacc gaatttttcg atacggacaa ctttccctag ataaacttca
    10681 ccaactttga cttctttggt caaatcttcg atgatcttga tagctttttt gatcatttct
    10741 gcatcagctg aagcaatact tacttttcca tcttgatcga tatcgatttt cacaccagtt
    10801 tcatcgatga ttccgttgat cgtatcgccg cctttaccga taacatcttt gattttagca
    10861 ggttcgatct ggatcatttc gatttttggt gcatattggc ttaattcttc acgtggagca
    10921 gcaatcgttg aagtcaattc ttctaagatt tccatacgtg cttgttttgc ttgagttaat
    10981 gcttcagtta ggatttgttc tgtgattcct tgaatcttga tatccatctg taaagcagtg
    11041 ataccatctt ttgtaccggc aactttaaag tccatatcgc caagatgatc ttccagccct
    11101 tggatatcag ttaggatcgt atagttttca ccatcagaaa caagtcccat cgcaataccg
    11161 gcgactggcg ctttgattgg tacacctgca tccatcaatg ctaatgtgcc agcacagata
    11221 cttgcttggg aagaagaacc atttgattcc aatacttctg ctactaaacg gatcgtataa
    11281 ggaaattctt cttctgaagg gattacttgt gctaacgcac gttcacctaa tgcgccatgt
    11341 ccgatttccc gacgccctgg agagcctgca cgtcctgtag aaccaacaga aaattgaggg
    11401 aaattgtagt ggtggatgaa tcgtttgctg acttccaccc ctaaaccatc aatgatttgg
    11461 tgctctccta gaggtgcgag tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac
    11521 aaacctgatc catgtacacg tggcaataag ctgacctctg atgacaatgg acggatttca
    11581 tccaatttac gcccgtcagg acggatctta tcgatggtga tcaattcacg tacgacatct
    11641 ttttcaagat cttctgtgat ttgtttaact tctttcaaca agtgatcttc atcttcatga
    11701 cctgcaaatt tctcagcata agcttcgcgg atatcgatct tcacctgctc gatattatct
    11761 tcgcgagcaa gtttttcttc agtcattact gctgctttca ttgtagcgta agaagcatca
    11821 aagatttctt ttttcaatac ttcatcaact tgcagtaaag tcacttccat tttttctttc
    11881 cctactgctt gaacgatttc ttcttggaaa gcaactaact ctttgatagc gtcaaaacca
    11941 aacagcaatg caccaagcat gtcttcttct gaaacttctt tcgcgccgct ttcaaccata
    12001 ttgatggctt gtttcgttcc ggcaacagtc aattcgatat ctgtttgttc tgcttgttct
    12061 actgttggat tcaagacgta ctcgccattt acacgaccaa cttctacgcc tgcgattggg
    12121 ccgtcaaatg ggatatctga gattgatagt gctaaagaag aacctagcat tgctgccata
    12181 gctggcgagc agtctgtttc tacactcatc acgatgttcg ttacttggac ttcattacgg
    12241 aagccttctg caaacatcgg gcgaattgga cggtcgatca aacgggcagt caatgttgct
    12301 tccgtacttg gacggccttc tcgtttgatg aaacctccag ggattttccc tgctgcatac
    12361 attttttctt cataattgat cgttaatggg aaaaagtctg tatcttttgc ttctttagag
    12421 gccacagctg cacttaagac tactgtatct ccataacgga ctaaaacggc tccgtttgct
    12481 tgttttgcta gctgaccgac ttcgatttgt aagggacggc cgccccatgt cgttttgaat
    12541 acttgttttt ctgacataac ttctcctttt ccttctcgtg aaagcaagac gctactaaac
    12601 aaatgaaaac tgccgaaagc cgtcttttga gcagctttca tttggttagt aggagtccat
    12661 ctagcttgat cacggaattt tacttaattg atatacgagt aaaaaaagtg agattcatgt
    12721 gaatcccact ctttttgact aaaattaacg acgcaagcct aaacgtttga tcaattcgcg
    12781 gtaacgttga acgtcagttt tacgtaagta agctaacaag ttacggcggt gaccaacttt
    12841 tttcattagg ccgcggtatg aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa
    12901 gtggttgatt tcttcagtta atacagcgat ttgtacttct ggagaaccag tatccccttc
    12961 atggcgagcg tattcgttga tgatttcatt cttgcgttct tttgaaattg ccatttcttt
    13021 cacctctttc gattattccc ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt
    13081 ggcaggtcat gtgcctagag cacacagcta tactttacct gaaaccgggt tgaaaatcaa
    13141 gtagttaatc attaagaaaa ggtagttttc aaacaatctt actactactt ttccattttt
    13201 gtggtaaact ataatgcgaa aatagacttg aaacacagtc ttttaagaaa ggggtacata
    13261 ttccatgatt acaatggatg atatcattcg tgaaggaaat ccgaccttac gcgaagttgc
    13321 aaaagaagtt tctcttccat taagcgaaga agatatctct ttaggaaaag agatgcttga
    13381 gttcttaaaa aacagccaag atccaatcaa agctgaagaa ctacacttac gaggcggtgt
    13441 tggcttagct gctcctcaat tagatatttc taaaagaatc attgctgttc acgtaccaag
    13501 tcctgaccca gaggcagatg gaccttctct aagtactgtt atgtataatc ctaaaatttt
    13561 gagccattct gttcaagatg cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg
    13621 agaagtacca ggatatgttg ttcgccatgc caaaatcact gtgtcttatt atgatatgaa
    13681 tggtgaaaaa cacaagattc gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat
    13741 cgaccatatc aacggtgtga tgttctatga ccacatcaac gatcaaaatc catttgcact
    13801 aaaagaaggc gtattggtga tcgagtagag aattggcagg ttccttcgat actatgctac
    13861 aagaaagctt tataaaaaag gggagtgctc aaaagctgca cttccctttt ttatgtttct
    13921 ccactaatcg aaatttagat agacatctag aaatcgcaat tccaaaaaat tctgtgtatt
    13981 ggatacgcat cttttcttat tttttcttat ttcttccata tgggtgttta tctcagagat
    14041 gatagaatag tttctaggat aaaaagtagc agctacgatg ttatccttcg tagaggattc
    14101 ctgcttcagc tagtttttgt ttgatgtttt tcagcatatg cttttctggg tagcggatcg
    14161 tatgcagatg gatgccgcct gttaattcgg ataacaaagt agcattactt ttttgataac
    14221 gtctcataaa ggaatcgata tcttgtggcg ttttgatgtg caaagttcct gctaactcac
    14281 cgtaaagggg atgttccaca attacatcaa tgatttcacc accattttca acgattagtc
    14341 tcagttcttc ttctgtttga tcttttccat gctggacagc gattttactg atgtgcccag
    14401 tattggtttc ttctgtttcg aggatataac ctcttgctgt tgccacgata cttttacctg
    14461 cagctcgtag taaagcaata tcgcctacaa tgatctgacg gctgactcca aaagttttcg
    14521 caaacttgct agcactcact ggtttttctg tatttgccaa ggtttgcatg atttgttttc
    14581 gccgtgtctc tccttcgatc acacttattc ctccttcatt ttgttttggt attcgcgcga
    14641 gtgtttcatc aaatcaacat acgtgtctgt tattgtttcc ttatatccct tatttttcag
    14701 cagactaaca actttatcca atacttgctg ctctcttttt gcatcataaa ctgcttggtt
    14761 ttcttctttt ttcacttgtc ctattttagt taccgtatcc attctttttt ctaataaagc
    14821 aaccaactct cgatcgatca tatcgatctt atgacgtaat gtttcaagag actcttctgt
    14881 tagttcttcc tctactgtct tgatgatacg agcaggattt cctgcaatca ctacattgtc
    14941 gggaaaagat ttggtcacaa ccgaacctgc gccgactact acgttgtccc ctagagtgac
    15001 acccggcgtg acgataactc ctccgcctaa ccaaacatta ttaccgattt taatgggttt
    15061 tgcgtactcc agaccgctgt tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag
    15121 ctggacattc ggagcgaaca tgcagttatc accgatttca atcgtgctga catctaaaaa
    15181 tgtacaattg aaatttgcat aaaaattctc cccaacaaat atattatatc cgtaatcaaa
    15241 attgataact ggctccatat agattttttt ccctgtacgg ccaaaagtct ctttcaacaa
    15301 ttgggatcgc agttccgctg attctgcttg gttgatgatt tggctttgac ttcttgcaaa
    15361 catacggtcg gcaaccagct caggatcatt ggcaaaataa agctcgcctg caatcatttt
    15421 ttctttttcg gttttgaatt cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa
    15481 caaccgctcc gtcttcttga ataaacgtat aaggctccgc taaataaaca ggcgtttgat
    15541 tgattactaa gattttggct gacggctggc gaaactgtac cagatcacaa aacggatgaa
    15601 cttgaaaact cgttccaaca atgacgatca gctctgcttg gctgacggca taagccgcct
    15661 gctccatgac tccatcttga aacccttcac catataacac gatgtctggg cgtatttgtc
    15721 ctccgcatgc ttgatgtttg tcgctgtgaa gatattcttt ccagtctacc gtttgttgac
    15781 attttcgaca atgacattga tacagactcc catgaaaatt gacgagttgc gggcttttag
    15841 cctttgcgtg caaaccgtcg atgttttggg aaacaaccca aacatttttt tctttcgcta
    15901 gatgtgctat ttccaaatga atgatattgg gttttgcttc aggatgataa atctttttga
    15961 caaattgata gaatttatcc ggttcattca ccattgcttg atgactcagc aagtattccg
    16021 gctgctccat cccgtggtag attcctgaca aagatcgata gtctggcact ccagaaggtg
    16081 tggatacccc tgcaccggtc aaaaatgtaa tttgctgata agctaatatc tcttttgctg
    16141 cttcgtctac tgttaattgt ttcatgtttt gtcacctcac tttccattat agcccatact
    16201 cgaacaaatt tattgttgaa aagtcagaaa aatgggaata aaaaagtgtt atgggcattt
    16261 gagcaattta tcacaatgtt cactgacaaa agaatgaaaa cgtgttacaa tactgctgta
    16321 taaaagtgag gtgaatcgat atgttaccat tctttattta ttggggcgtt attctggctt
    16381 gtatcgcctg gttagcgtta agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa
    16441 atggaaatct ttgggctttt gctttattca acgtgattgc cgcaattgtt ttagccatta
    16501 cattagccgt atatcgaaca tggggctggg gaatcaccca atactcaagt ttgatttatc
    16561 taatattagc catttatggc gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa
    16621 agaaagcggc agcttaaatg atcgagatgg aactggacac aggaaatttt ctgttctagt
    16681 tcttttttta ttgtattaca tacatttttg tgtataatta tactatcgtt tgcaatggat
    16741 agatgattca cattttgatt gttttttcgg taaaagtaaa gatttctata tttatcagta
    16801 ttttttcaaa aaccagcagt tatagattga caaagcctct cgagtatcgt aaactttttt
    16861 tgtataaata actgaatacg agatgcctca atcaatgagg tagaggtcgc gagaatcatt
    16921 aaccctgtgg agtgaagcaa acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc
    16981 acttttttgc ttggagtgta tgttgggacg caagttaaga gcttgcggac tgtcttagca
    17041 gtgatgctaa gttgcgctat gttttgtgag aggtttatca ccttatggaa ttttagcggc
    17101 ctttttatat ctataaatcg gtcgcttttt attttttcag ataaagcttt accgcatttc
    17161 atctagaccg ctcgtgtatc atataatcag aaagggagaa aaaaatgaaa aaaaatatgg
    17221 tttcagttgc cgtactattt gcttttgtct tctcactatt cagtgtggga tctctggcaa
    17281 atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg tatggaggct gggtatgcac
    17341 ccttcaactg gtctcaacaa acagatgaga ataatgcctt gccaattcaa ggccaaaata
    17401 gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa
    17461 aattagtcat cgtccaaacg aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga
    17521 tcgacgcgat catcgccgga atgagcccta ctgctgaacg acgaaaagaa attgatttta
    17581 ccgatcctta ttacgaatca caattagtag tagtagttca aaaagatagc aaatatgcaa
    17641 aagcaaaaag tttagcagat ctttctggag caaagatcac tgctcagtta aatacattcc
    17701 actatactgt gatcgatcag ataccagatg tgcagaaaca acaagcaatg gataatttct
    17761 cagcaatgag aacagcttta gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg
    17821 aaggtgtaac ggcaactagt gtgaacaaag acttggaaat gcttgaattc agcggtgcag
    17881 atggcttcca gacaaacccg gaagatgttc agatttctgt tggtatgcgt aaaggcgacc
    17941 cggatatcca aaaagtaaac cagattcttt ctggcatctc taacgatgaa cgaaccaaaa
    18001 ttatggacca agcagtcaaa gatcagcctg ctgcaactga tagtgaagaa aaacctggca
    18061 tcatttctga tttcactacg atttggaaac aatacggcaa tatgttctta cgaggtgctg
    18121 gacttacctt gttcatcgca ttgatcggga cagttgtcgg tactacactt ggattactga
    18181 ttggtgtctt ccgttcggta cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa
    18241 tcggaaatgc acttctttct atctatgtcg aagtattccg cggaacaccg atgatggtac
    18301 aagcaatggt catcttctac ggtctggcac tcgcttttgg catttcattg aaccgaacga
    18361 ttgccgcttt gtttattgtc tctctcaata ctggagctta catgacagag atcgttcgtg
    18421 gggggatttt tgcagtagat gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc
    18481 atggccaaac gatgagaaaa gttgttattc cacaagtttt acggaatatc ttgccagcaa
    18541 caggaaatga gtttgttatc aatatcaaag atacagcagt tttaagtgta atcggcgtag
    18601 ctgatttgtt cttccaagga aactctgctt ccggtgcgaa tttccaattc ttccaaacat
    18661 ttacgatcgt cgggatcatc tacttagtca tgacctttac gatcaccaga atcctgcggg
    18721 cggtagaaaa gaaaatcgat ggtccttcag cttataccaa gcttgaagaa atcgacaatt
    18781 caaatttgca ggaataagga ggaagaatca tgaatgaaaa aattattgaa atcaaacatc
    18841 taagaaaaag cttcggtgaa aatgaagtat tgaaagatat cagcgtaaca gtcaataaag
    18901 gtgaagttgt gacgatcatc ggttcttccg gttctggtaa atctactttc ttgcgctgta
    18961 tcaatttact ggaaaaacca acaggcggcg agatcattta taatggagaa aatgtattag
    19021 ctcctaaata taatttacct aaatatcgaa cgaatctagg tatggttttc caatccttca
    19081 acctgttcaa caatatgaat gtcttggaaa actgtatgtc tggtcaaaca actgtcctga
    19141 aacgagataa agaaactgcc aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg
    19201 atcgttatat cgaagccaaa ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa
    19261 ttgctcgggc attatcaatg aatccagatg tattgctatt cgacgaacct acttcggcgt
    19321 tagaccctga aatggttggc gaagtgctta atacgatcaa agacttagca catactggct
    19381 tgacaatgct tatcgtgacg catgagatgg aatttgcaaa agatgtatct gaccgtgtca
    19441 tttttatgga taaaggtgtg attgcagaag aaggtacacc agaagatatc tttgtccatc
    19501 cgaaagaaga acgtacaaaa gaattccttt cacgtatttt aaatgcttag gatcaatagc
    19561 tgattgggac aaagagctgc ggtgtttacc gcagttcttt tttatgaatt actcataaaa
    19621 ggtaaatttc tgaaaatttt ggaatcttag ttgtgttttt cagaaaactc tcttaatatg
    19681 gaagtaatta aactttttta gttaggatga tttaaaaatg aatgaagagc aagaactgca
    19741 gcgtggactg aaaaatagac atgtccaact gatttctatt ggcggagcga ttggaacggg
    19801 gttgttttta ggttccggaa agtctatcca gctagctggc ccttcgatat tgctcgccta
    19861 tcttattact ggatgcatct gttttttcat tatgcgtgcg ttaggcgaac ttctgttatc
    19921 aaacaccaat aaccattctt ttctggattt tgttgcagaa tatcttggca aaaaagcggc
    19981 tttcatcact ggctggacgt attggttctg ctgggtatcg attgcaatgg ccgatttaac
    20041 agccatcgga atgtatgtac gctactgggc gcctggtgtt cctcagtggc ttcctgaact
    20101 gattgcatta ggtcttcttc tttgcttgaa tatggtggct gtctcattgt ttggtgagtt
    20161 agagttttgg tttgcattga tcaaagttgt agcgatcatt gcctttatcc tcgtaggagc
    20221 ttatatgatc ctcacccact atacgacaga tattggcaca gccagtatca cgaatctatg
    20281 gtctcatggc ggattctttc caactggtgc aaaaggattt atcttagctt tccaaatggt
    20341 aacctttgct tttgctggga tagaattagt tggattagtc gctggagaaa cagaaaatcc
    20401 tgaaaaagtg ttgccggaag ctatcaataa tattcctatc aggatcatct tattttactt
    20461 aggatctctt tttgtcatta tggcaatcta tccatggaac agcttgaatc cagataacag
    20521 tccatttgta gaagtatttt cggaaattgg gatcacgatt gctgcttcct tgatcaattt
    20581 agtcgtatta agtgcagcag cttctgcctg caacagtgca atatatagta caggacgtat
    20641 gcttcgttct ttagcgcaag aaggcagtgc ccctaaaaaa ttcaaaaagt taacaacaca
    20701 tcatgtgcca gggaatgctt tgacattttc tactatcgtc attttcattt ctgttatctt
    20761 gaattacgtg atgcctagtg aagtattcac actagtaagc agtattgcca ctacttgttt
    20821 cttgtttatc tggagtatgc ttgtctacac gcacatgaaa tatcgtaaaa gtatcctcgg
    20881 caaaaaagct cattcattta aaatgccgtt gtacccattt agtaattatc tggtatttct
    20941 ttacatggcc tttgtgtgtg tcgtcttgtt cctaggaaaa gatacgagaa tcgctttatt
    21001 actaacacct gtgtggttct tgcttctatt actaatctat catatgaaat atgaacgtaa
    21061 gaagtaagaa agtagttgtg acagaagtgg tcagcttcga gaaataagaa ggaatttacg
    21121 aaaattgctt cttaaatttt tgtgaaattt cggcttattt cttggagata aaagcttctg
    21181 tcccagcctc ttttctattt ccccggaaac aaccgctgat aataggcatc tgctttttta
    21241 taggaaaaaa tccaaatacc agatacaaaa aaacgtccga ataacccgat caacggtaca
    21301 aaaaagacac cggcaatcaa gcataagtta atcatcatgg agataagact tcgacggtcc
    21361 tttgacaaaa ctctcacgac atgggaatct ggtttatttg ctttcatgat ttgcctcatc
    21421 aagataccaa aagaaaaact ccacatcgtg tatactaaga cataagccat ctcaggccaa
    21481 aaagcttcag ggttacgccc gacccaacta gtaacagaag gacataaagt cagccaaaag
    21541 agcaaaaaga tattcgacca gaccactcgg taattaattc gttctgccat ctgaaatata
    21601 cgatgatggt tcacccagat caccgcaaca aatataaagc tgatggtata agctaataat
    21661 gtatttcgga tgacccataa agaatgaaag ctagatgtat tagggatctt gatctctaat
    21721 acaagaactc tcaaaacaat ggctaccact ccatcagtaa atgcttctaa tctcgacttt
    21781 gtcacgatga ttcctcctca gatgattcag cgcttttatt ttatcactta cgttattaaa
    21841 taaccatcgt tttatatagg gaattttctg aaaattgttg catatttttc gaaattttgt
    21901 tacttttaaa aaaaccgtaa aggagttatg tatgacaaaa aaattatcat ttaaagacta
    21961 tctttttata ggctccatgc ttttcggtct attttttggt gctggaaatc ttatctttcc
    22021 cgttcatttg ggtcaagaag caggcgctgc taccttttgg gcgaatctag gtttcttggt
    22081 taccggtatc ggtcttccct tcttaggggt gattgccatt ggtgtatcga aaagctcagg
    22141 tgtatatgaa ttagcaaaac ggataaatca aacatatgcg atggttttta ccatcttgct
    22201 ttacttagtt atcggaccgt tttttgcttt acctcgcctt gcaaccacat ctttcgagat
    22261 tggtttagca ccatttatcg atcctagcag acagacgcta tatctagcaa ttttcagtat
    22321 tgtatttttt gtgctggctt ggtggttttc aagaaaaccg acaaagatat tagattatgt
    22381 tggaaagttc ctgaatcctg catttttggt tttattaggt gctttgcttc tccttgcttt
    22441 tttgaatcca ctaggttctg taaatcaagc gcctattcag ccaaattatc aagagcatgc
    22501 ttttttcaca gggtttactc aaggatataa cacattagat gcgcttgctg ctttagcatt
    22561 tgggatcatc attgtcacaa caatccaaaa catgggtgta acaaaacctg ctgaaatcgc
    22621 taaagacacg atcaaatctg gtgcgatcag cattatattg atgggaatca tttatacttt
    22681 gctcgcttat ttaggtgcta tgagcttagg tagctttgct ctaagtgaaa atggcgggat
    22741 cacgctggct cagattgctg atcattattt aggtacttat ggcagtatct tattagcatt
    22801 tatcgttata ttagcttgct tgaagaccgg tattggtttg attacagctt ttgcagaaac
    22861 attcacagat ttgtttccgc aaagaaatta cgcattcttt gttgcacttg ctagtatcct
    22921 tccttgttta gcggcaaacg taggcttgac gaatatcatt caattttcgt tgcctgtttt
    22981 gatgttcatc tatccattag caatgacact gattttacta gtgttagttg gtcctttgtt
    23041 taagcagcgt acggctgttt atcgaatgac tacttatttt acattgattg cttcgatttt
    23101 tgatgggttg aacgcttgcc cagaaactat caaacaaaca cctattgttc aaaatctttt
    23161 atatgttgcg gaaagctact tgccgttttt caaattgggg atggggtgga tcgtaccagc
    23221 agttatcggt tttgttatcg gactgatttg gagtttcgct aagaaagaag aagtagcaga
    23281 ttgataaaag aggttgtgaa agagacgttc agccttgagc aataaaaagt aattttgaaa
    23341 aatggcttct cacatttttg cataaattta acttaatacc gagaaactga cttttgaaca
    23401 ccgtttattg agaggtcgtg aaaaagccgc ttagtttcaa gtattaagaa atattttgtg
    23461 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23521 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23581 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23641 attt
//
LOCUS       DACCIQ010000038        23626 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCIQ010000038 DACCIQ010000000
VERSION     DACCIQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.038"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            72..1412
                     /locus_tag="IVM62_001497"
     CDS             72..1412
                     /locus_tag="IVM62_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845463.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1630..2073
                     /locus_tag="IVM62_001498"
     CDS             1630..2073
                     /locus_tag="IVM62_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6845464.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="IVM62_001499"
     CDS             complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="IVM62_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6845465.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4398..5621)
                     /locus_tag="IVM62_001500"
     CDS             complement(4398..5621)
                     /locus_tag="IVM62_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP6845466.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5652..6491)
                     /locus_tag="IVM62_001501"
     CDS             complement(5652..6491)
                     /locus_tag="IVM62_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6845467.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6508..7188)
                     /locus_tag="IVM62_001502"
     CDS             complement(6508..7188)
                     /locus_tag="IVM62_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845468.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7190..8263)
                     /locus_tag="IVM62_001503"
     CDS             complement(7190..8263)
                     /locus_tag="IVM62_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6845469.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8910..9581)
                     /locus_tag="IVM62_001504"
     CDS             complement(8910..9581)
                     /locus_tag="IVM62_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6845470.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9668..12346)
                     /gene="mgtA"
                     /locus_tag="IVM62_001505"
     CDS             complement(9668..12346)
                     /gene="mgtA"
                     /locus_tag="IVM62_001505"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP6845471.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13005..13442
                     /gene="ndk"
                     /locus_tag="IVM62_001506"
     CDS             13005..13442
                     /gene="ndk"
                     /locus_tag="IVM62_001506"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP6845472.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13464..14330)
                     /locus_tag="IVM62_001507"
     CDS             complement(13464..14330)
                     /locus_tag="IVM62_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6845473.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14490..16583)
                     /locus_tag="IVM62_001508"
     CDS             complement(14490..16583)
                     /locus_tag="IVM62_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6845474.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17332..17955)
                     /locus_tag="IVM62_001509"
     CDS             complement(17332..17955)
                     /locus_tag="IVM62_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845475.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18114..19511)
                     /locus_tag="IVM62_001510"
     CDS             complement(18114..19511)
                     /locus_tag="IVM62_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6845476.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19543..20514)
                     /locus_tag="IVM62_001511"
     CDS             complement(19543..20514)
                     /locus_tag="IVM62_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6845477.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20888..21313
                     /locus_tag="IVM62_001512"
     CDS             20888..21313
                     /locus_tag="IVM62_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845478.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21801..22502)
                     /locus_tag="IVM62_001513"
     CDS             complement(21801..22502)
                     /locus_tag="IVM62_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6845479.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22518..22958)
                     /locus_tag="IVM62_001514"
     CDS             complement(22518..22958)
                     /locus_tag="IVM62_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6845480.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22958..23533)
                     /locus_tag="IVM62_001515"
     CDS             complement(22958..23533)
                     /locus_tag="IVM62_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP6845481.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6793 a         4899 c         4064 g         7870 t
ORIGIN      
        1 gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa
       61 agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca
      121 tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg
      181 ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg
      241 tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg
      301 gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt
      361 ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt
      421 ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca
      481 tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa
      541 tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg
      601 gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg
      661 gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac
      721 tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca
      781 aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc
      841 tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa
      901 aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct
      961 catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc
     1021 gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg
     1081 ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca
     1141 aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta
     1201 tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca
     1261 gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca
     1321 ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa
     1381 agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat
     1441 gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt
     1501 tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa
     1561 aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt
     1621 ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt
     1681 atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca
     1741 cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg
     1801 tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt
     1861 tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg
     1921 aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa
     1981 gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac
     2041 aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag
     2101 gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga
     2161 ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2221 cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     2281 ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa
     2341 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2401 tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa
     2461 tggctttcca catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc
     2521 gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat
     2581 tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt
     2641 ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg
     2701 aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt
     2761 ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca
     2821 gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca
     2881 gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa
     2941 atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag
     3001 ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc
     3061 atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt
     3121 gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga
     3181 ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc
     3241 tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta
     3301 aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt
     3361 gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac
     3421 atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt
     3481 gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca
     3541 accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt
     3601 gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca
     3661 gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg
     3721 atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg
     3781 atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt
     3841 tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag
     3901 acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt
     3961 gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt
     4021 cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct
     4081 cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc
     4141 ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc
     4201 atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac
     4261 acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt
     4321 tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca
     4381 cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct
     4441 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
     4501 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
     4561 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
     4621 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
     4681 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
     4741 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
     4801 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
     4861 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
     4921 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
     4981 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
     5041 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
     5101 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
     5161 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
     5221 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat
     5281 tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
     5341 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta
     5401 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     5461 aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     5521 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     5581 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     5641 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     5701 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     5761 gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     5821 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     5881 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     5941 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     6001 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     6061 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     6121 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     6181 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     6241 aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg
     6301 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     6361 tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt
     6421 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     6481 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     6541 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     6601 tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac
     6661 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     6721 gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc
     6781 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     6841 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     6901 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     6961 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     7021 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc
     7081 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     7141 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     7201 cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac
     7261 cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa
     7321 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     7381 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     7441 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     7501 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     7561 cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt
     7621 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     7681 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     7741 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     7801 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     7861 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     7921 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     7981 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     8041 tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     8101 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     8161 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     8221 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     8281 taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg
     8341 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     8401 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     8461 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     8521 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta
     8581 ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt
     8641 ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa
     8701 cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct
     8761 tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt
     8821 tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa
     8881 gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt taacatttca
     8941 ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg
     9001 gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa
     9061 gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct
     9121 gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct
     9181 ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct
     9241 cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc
     9301 aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa
     9361 tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact
     9421 aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg
     9481 atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg
     9541 tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc
     9601 aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc
     9661 ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa
     9721 gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca
     9781 tcaagcctaa ttcagcacca aaccttgtaa aaggaaggac agaaccgaca ataattccta
     9841 atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg
     9901 tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat
     9961 ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca
    10021 ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac
    10081 ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct
    10141 cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga
    10201 tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg
    10261 caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca
    10321 tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt
    10381 tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa
    10441 taccgatatc agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga
    10501 cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt
    10561 ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga
    10621 ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg
    10681 taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt
    10741 tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa
    10801 ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg
    10861 cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt
    10921 cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt
    10981 tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa
    11041 ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat
    11101 tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat
    11161 ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt
    11221 catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg
    11281 ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga
    11341 tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca
    11401 ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta
    11461 gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa
    11521 atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt
    11581 tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga
    11641 ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg
    11701 tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc
    11761 ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg
    11821 gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa
    11881 tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt
    11941 tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa
    12001 ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat
    12061 attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt
    12121 atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac
    12181 cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga
    12241 atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat
    12301 ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct
    12361 tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga
    12421 tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt
    12481 gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt
    12541 tatttttcca attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa
    12601 aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct
    12661 cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc
    12721 taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt
    12781 ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc
    12841 cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac
    12901 tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc
    12961 acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg
    13021 ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg
    13081 aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa
    13141 aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga
    13201 cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta
    13261 agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg
    13321 cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag
    13381 agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt
    13441 gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc
    13501 tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg
    13561 tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc
    13621 atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg tttcatatct
    13681 tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc
    13741 attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt
    13801 tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca
    13861 gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg
    13921 attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc
    13981 atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta
    14041 ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt
    14101 tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt
    14161 gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa
    14221 tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt
    14281 gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc
    14341 ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac
    14401 gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt
    14461 gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct
    14521 gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga aaagacactt
    14581 gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag
    14641 tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt
    14701 tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag
    14761 atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa
    14821 gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact
    14881 acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg
    14941 attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg
    15001 actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg
    15061 ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta
    15121 tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata
    15181 ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt
    15241 cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg
    15301 tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt
    15361 tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa
    15421 cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag
    15481 tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt
    15541 gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca
    15601 agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca
    15661 gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct
    15721 tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta
    15781 atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc
    15841 ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca
    15901 atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca
    15961 taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc
    16021 tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct
    16081 gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt
    16141 ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg
    16201 acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa
    16261 aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc
    16321 aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt
    16381 ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc
    16441 cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca
    16501 atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg
    16561 cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt
    16621 tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt
    16681 ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac
    16741 taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt
    16801 tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc
    16861 ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct
    16921 cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt
    16981 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    17041 ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa
    17101 agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa
    17161 attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    17221 aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    17281 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc
    17341 ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc
    17401 acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt
    17461 tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa
    17521 acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt
    17581 tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc
    17641 tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg
    17701 attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt
    17761 tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc
    17821 tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga
    17881 aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg
    17941 tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct attatataca
    18001 aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga acttcttttt
    18061 cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct ttcttaagcc
    18121 tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt gattgttctt
    18181 tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata tgcattggtc
    18241 cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc accttcttga
    18301 atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata atcatcgtgg
    18361 atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc gttttcagaa
    18421 ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc atagattcct
    18481 tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat ttcataattg
    18541 tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc ttttgctttt
    18601 acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc tttgataacc
    18661 gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat ctcaatcaat
    18721 tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa gaacgcatct
    18781 gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata gcttggtgtc
    18841 agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga tttgatggct
    18901 aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat atctgactct
    18961 tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg ttcattatga
    19021 gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc atatttttca
    19081 taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata catcggcata
    19141 tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc atcaaaataa
    19201 cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg tatcaaacgg
    19261 ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc
    19321 tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt
    19381 tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc gcctgaaatc
    19441 cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc tggaaattga
    19501 tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga ttccatgtat
    19561 gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga ttcgttttaa
    19621 cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg tgattttcgg
    19681 aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa tatccttccc
    19741 gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat tgtaattgct
    19801 gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat agccgctttt
    19861 tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt ggaccgcttc
    19921 tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag caataaatcc
    19981 gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac tttctcgatc
    20041 attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg cgataactgg
    20101 aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata agaagacaat
    20161 cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt tttctggctg
    20221 atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc tttcgatccc
    20281 tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc caatagagaa
    20341 gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca acgctgaaat
    20401 cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca agtaacggga
    20461 aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg ccatacattc
    20521 tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga tgaccgcatt
    20581 ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag tagagcaact
    20641 tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt taatatagca
    20701 tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg gattttttgc
    20761 ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc gtttttctct
    20821 tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat caaatggggg
    20881 aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt tgacattgac
    20941 tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg attcttctgt
    21001 cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag tacgtaaaga
    21061 tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat ggacacgcac
    21121 gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag tgctgacaga
    21181 tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa ataaaaaaga
    21241 aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg atggcaacat
    21301 ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct tttctgctat
    21361 agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt cctgattatt
    21421 gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga agcttgagca
    21481 tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa tcttatcctg
    21541 ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact cactatggct
    21601 gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca gtaaaacaaa
    21661 cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc aaatatttga
    21721 atataaaata accaaacgat agaaaggctt attctacatc acgtagtaat ttttctttcc
    21781 cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg gttatccgta
    21841 aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat ctgacggatc
    21901 accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc cacttgtatt
    21961 ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa cgtttcataa
    22021 taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg ttttttgata
    22081 cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg ccgttttttt
    22141 ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta ctgctgtact
    22201 tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct atcttcatag
    22261 atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc taaaaatcgc
    22321 ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga ataaaagagc
    22381 ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat ctcttttgca
    22441 agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa aatcactgac
    22501 atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg caatgtttca
    22561 aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact ttgtgtaatc
    22621 atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt caacatagtt
    22681 gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa gtcaaggata
    22741 gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac ttcctgcgcc
    22801 catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga gtagaaactt
    22861 gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc gatttgctcc
    22921 tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc ttcaataata
    22981 actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga ttgttggaaa
    23041 agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa gtttctaata
    23101 attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc ttgtcgggcg
    23161 tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta cctagacctc
    23221 cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt tcatcgatcg
    23281 tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca aaaagcccat
    23341 tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta atcactccca
    23401 taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc
    23461 ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa
    23521 cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt
    23581 ttttttaaaa taaaagacta gattttgtag ttttttagtg gtatat
//
LOCUS       DACCIQ010000039        23057 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCIQ010000039 DACCIQ010000000
VERSION     DACCIQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.039"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            82..2523
                     /locus_tag="IVM62_001516"
     CDS             82..2523
                     /locus_tag="IVM62_001516"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP6845482.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2853..3557)
                     /locus_tag="IVM62_001517"
     CDS             complement(2853..3557)
                     /locus_tag="IVM62_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845483.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3744..3989)
                     /locus_tag="IVM62_001518"
     CDS             complement(3744..3989)
                     /locus_tag="IVM62_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845484.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4006..4857)
                     /locus_tag="IVM62_001519"
     CDS             complement(4006..4857)
                     /locus_tag="IVM62_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6845485.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5023..6027
                     /locus_tag="IVM62_001520"
     CDS             5023..6027
                     /locus_tag="IVM62_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP6845486.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6268..6624)
                     /locus_tag="IVM62_001521"
     CDS             complement(6268..6624)
                     /locus_tag="IVM62_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6845487.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6992..8179
                     /gene="tuf"
                     /locus_tag="IVM62_001522"
     CDS             6992..8179
                     /gene="tuf"
                     /locus_tag="IVM62_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6845488.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8367..10958)
                     /gene="mprF"
                     /locus_tag="IVM62_001523"
     CDS             complement(8367..10958)
                     /gene="mprF"
                     /locus_tag="IVM62_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6845489.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10984..11703)
                     /locus_tag="IVM62_001524"
     CDS             complement(10984..11703)
                     /locus_tag="IVM62_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6845490.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12023..12544
                     /locus_tag="IVM62_001525"
     CDS             12023..12544
                     /locus_tag="IVM62_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP6845491.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12603..13463
                     /locus_tag="IVM62_001526"
     CDS             12603..13463
                     /locus_tag="IVM62_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP6845492.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13570..15963
                     /locus_tag="IVM62_001527"
     CDS             13570..15963
                     /locus_tag="IVM62_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6845493.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16145..17986
                     /locus_tag="IVM62_001528"
     CDS             16145..17986
                     /locus_tag="IVM62_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP6845494.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18010..19119
                     /gene="rpoD"
                     /locus_tag="IVM62_001529"
     CDS             18010..19119
                     /gene="rpoD"
                     /locus_tag="IVM62_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP6845495.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19339..21438
                     /locus_tag="IVM62_001530"
     CDS             19339..21438
                     /locus_tag="IVM62_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6845496.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21634..22497
                     /locus_tag="IVM62_001531"
     CDS             21634..22497
                     /locus_tag="IVM62_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6845497.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22602..>23057
                     /locus_tag="IVM62_001532"
     CDS             22602..>23057
                     /locus_tag="IVM62_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6845498.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7764 a         4037 c         4807 g         6449 t
ORIGIN      
        1 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
       61 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
      121 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
      181 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
      241 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
      301 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
      361 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
      421 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
      481 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
      541 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
      601 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
      661 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
      721 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
      781 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
      841 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
      901 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
      961 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     1021 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     1081 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     1141 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     1201 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     1261 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     1321 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     1381 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     1441 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     1501 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     1561 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     1621 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     1681 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     1741 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     1801 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     1861 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     1921 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     1981 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     2041 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     2101 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     2161 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     2221 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     2281 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     2341 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     2401 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     2461 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     2521 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     2581 atcttgcttt tttctctatc agaaaatatg tttattatta gaatttttta tagttaaggt
     2641 taggcatctt aagaaatcag gcaactccaa gatatcttca ggtagaagag aagtcgccta
     2701 acattgtagg atcataggtg atttgttgga caggtcaagc tgatatttga gcctgatgtt
     2761 agaaggaatt tctttttact tgctgtagaa attattatat gactaaaaag gctgggattt
     2821 taaatcccag ccttttatga caaatagaat ttttatcgat cagtaatcgc tgtcgctgca
     2881 aattgttcga tcaaaggaat caaagtcaat ttgactttgt atttccgttc gagtgctttt
     2941 tcgataacga actcattttc agtagtcata gaaatagttg ctaatttacg aggttcatca
     3001 gcattttttt cattaataac tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac
     3061 tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt
     3121 gtattttcag ttgtgtccgc ttttggtttt tccgtttttt cttctggcac actgactgtt
     3181 tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt catcgttttt caatcgttga
     3241 tcattccagt aagacacgat ttcaataaga agattgtcac ggaatcgttc gaaaatcccg
     3301 ttttctcctg aacgaattct ctttaataca gtattgaagt tgtcatcgat tggcacgttg
     3361 atccgtgtat cttcatttgt tttcccagct aatccgatcg ttaagatttg acggtttttg
     3421 ggatcaagca tgacttgtaa cggcaaacta taacgtcgca agctctcttc ttctagagaa
     3481 cggaataatt gtcctcttac accatccaat gaaggacgga attgatttaa taatttttgt
     3541 ttctgttttt tcttcatatg ttctctcctt cgctcatacc ctttcgatta taagaaaaaa
     3601 acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa
     3661 aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa atggaacgga ttatgtatat
     3721 atacataatc cgttccattc tttctatttt ttcggtatca aggcaaaaac aggtagttca
     3781 ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt
     3841 tttttcagtt cttcgatgat ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt
     3901 ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag
     3961 agagaattca agcgttcgat tggatccata aaatcacctt tcgacttaaa attgggtagt
     4021 atctgggtct tttgctttgc cgactacacc atctaactga tcgatttgtt tcatttcttc
     4081 ttctgttaat tcaaaatcaa ataattcagt attttctttg attctgcttg gtgtcactga
     4141 cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt
     4201 cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca tcgaagattt taccagtgcc
     4261 taatggacta tatgcttcga ggatcatatc atgttttttt gcatactcaa ctacagttgt
     4321 ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc ggcataattt ttgctgtttt
     4381 tgctagtgta tctagatggt ggggtaagaa attgctcaca ccgattgctt tgattttacc
     4441 tgcttcatac agttcttcaa atgctcgcca tgtatctgca tttgcttgtt cccaattatc
     4501 acgaaaagca acagggtttg gccagtggat caaaaacaga tctaaataat ctgtttggag
     4561 cttcttcaat gattcttcaa aagaagacat cacaagttca tagctgtggt ttgcattcca
     4621 taattttgtt gtcaagaata tctcttctct aggaattcca ctttccttaa tcgcttggcc
     4681 gacgctttct tcattcttgt atccttgggc agtatcgata tggcgataac ctgcagccag
     4741 cgcttcttta acagaggaaa cagcgacatc cccatcaggt gtttgccatg tgccgaatcc
     4801 tactaccggg atttcgtaac catttgatag acgataagtg cttgttaatg aattcatact
     4861 ttcagctcct tttttattcg ttcaactata agtgtagcgc atttttcgaa aaaaagaatc
     4921 cattttgttt ctactcttga aatttttgtt taaaagaaaa tgattttgat tctggtattt
     4981 ttgatttttt ttccgtaaac taagacagaa gggaatgaga aaatggatat ctatttaaaa
     5041 aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc acaaaaagag
     5101 ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca aaaaatctct
     5161 tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga atcacttaaa
     5221 atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc
     5281 taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgtcttgta tgaattagat
     5341 acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac tcactttgtt
     5401 gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat tctaggggcg
     5461 aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac ctacgaatta
     5521 attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg aagagtgatc
     5581 gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa agtagccaag
     5641 catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt gaaagaagct
     5701 gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc
     5761 ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga gcaaggattt
     5821 gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa atttggaaaa
     5881 caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac
     5941 ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat gatcaaaaat
     6001 gtggatgaag tattgaatcg tttataaaaa tgactagaat attaggtgtt caagtaccag
     6061 tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa
     6121 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     6181 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     6241 tatctaataa aattttaaga ccatttacta attatcgatt ttttctgccg catttttagg
     6301 cacttcagaa gcctttattt cttcccagct taatacacct ttacgaggat tgactttgag
     6361 tttaagatac gcattcatct ttaatggatg ttctctttct tcgcgaagcg tcacctgttt
     6421 tttttcacca tctttattat aagccgcttg cttgtaggta taagtcgtga atttctctcc
     6481 gttatctgct tgggtagctg acatttcgcc attcgtcgtg atttttgtat aataagcttc
     6541 gccgccgtaa aaataatgat aagctgcaaa acttccgccg agaaatatgc ctaaaacgac
     6601 aagtacgcca attaattttt tcatgtactt cacctctttc cttattatta acgaaataga
     6661 agatttcgtc tattgaaagt tgttggcaac agctgaattt taaacttttc ctcattttgt
     6721 tcttattgct tcaaaagaaa gttaaggact atcttgaagt ttttctcaaa aaacacattt
     6781 ttttatttat aaaaggtact tttttcgcgt ataagacaga cgagtgctct tagttgactt
     6841 tgaccaatat tgacgtataa taagcttagt tcgaatctta caaaaagtaa gtcggacgta
     6901 ttctttttga tttagtcaaa aaaagactat aataaaaagg tgagagctga aatttttcag
     6961 tgatctatat tatttaggag gtttgtaaat tatggcaaaa gaacattatg acagaagtaa
     7021 accacacgtt aacattggta cgattggaca cgttgaccat gggaaaacta ctttaactgc
     7081 tgcgatcact acagtattag gcaaaaaagg cctagcaaat cctcaagact atgctagtat
     7141 tgatgcagct ccagaagaac gtgaacgtgg gattacgatc aataccgcac acgtagaata
     7201 tgaaacagaa aaacgccact acgcgcatat tgatgcacca ggacacgcgg actatgtgaa
     7261 aaacatgatt acaggggcag cacaaatgga tggtgcaatc ttagttgttt ctgcaactga
     7321 cggtccgatg cctcaaacac gtgaacacat tttattgtca cgccaagttg gtgtaaaata
     7381 cctgattgtt ttcttgaaca aagttgattt agtcgatgat gaagaattga tcgatttggt
     7441 agaaatggaa gttcgcgagt tattgagcga atatggtttc ccaggcgatg acactcctgt
     7501 gatcaaaggt tccgcattaa aagcattgca aggcgatcca gatgctgaag ctgctattat
     7561 ggaattgatg gatacagtag atgaatatat cccaacacca gaacgtgata cagataaacc
     7621 attactattg ccagtggaag acgtcttctc aattacaggt cgaggaactg ttgcctcagg
     7681 tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta gagatcgtag ggatcaaacc
     7741 tgaaacacaa aaagcagttg taacaggtgt agaaatgttc cgtaaaacgt tagattacgg
     7801 ggaagctggg gataacgtag gcgtgttgtt acgggggatc caacgtgacg atatcgaacg
     7861 tggacaagta cttgctaaac caggttccat tactccacat acaaaattca aagcagaagt
     7921 gtacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcaaca actatcgtcc
     7981 acagttctac ttccgcacaa ctgatgttac aggaacaatc acattgccag aagatacaga
     8041 aatggtcatg cctggtgaca acgtgacgat tgatgttgac ttgatccatc caatcgctgt
     8101 tgaaaacgga acgacattct caattcgtga aggcggacgg acagtaggtt caggtatcgt
     8161 tacagagatc gaagcataag aaacgcgact cgaatatttt ggtaatgaaa aatgtttaaa
     8221 ataaaagagc ggtccaaaca ggatttctgt ttggaccgct ctttgttctt caattgtata
     8281 agaagagatt gtagaagcat gaactttcgt tttacttcag tctcttttat tcatcatgag
     8341 agtgttcatc tatcggtatg cagtttttaa tactttcttc gtatccattt tttcaaacca
     8401 cttattctac cttgtgtctg ctcaactggc gcattatcaa taatcagcaa agcaatcata
     8461 acatatgcaa tccaactatc tctagaatat agagtataac gtgagaccca gtttgtagcg
     8521 tacttgcttt tatattctct taatccatgg aaagaataaa aatgagaacc aaactcgtaa
     8581 accaagtatg caatcctttc ctgtaaaaaa ctctttctgg aagttccgac atttgaaaga
     8641 ggggccatcc ctaaattgaa ccattggata tgttcttctt tcatataggc aaacaaatgc
     8701 aagaacaaga aatccatact gccagaaggt gcttttttag aatcatatcg catcagatca
     8761 atcgttccta ctttattgtt cgtgtaagta ggcatgatcg ttgcaaaagc gacaatttct
     8821 tcttgggtat ttttgacaac tgcaataggc gctctttgta gataatcttc agagaaaaag
     8881 cctaaagaaa accctttttc ctttcgtgaa cccaaccaat tatcagagat atttttgaaa
     8941 gtactcattt gctcagtaga aaatggtggt tgtaagacat caaatgtaaa tccttctcgt
     9001 tcgatccgat tcaagacagc acgagtgccc ttcatttttt tccctgaagt tgtaaaacta
     9061 ttcaggtcta cataagcttc ttcccccatc ttaatgaaat cgtagccgaa ttcatgcaaa
     9121 atcataacaa tctcttcgct tgtttcgtag aaaactggta aatagcatag tcgatctgtt
     9181 tcttcgataa aagcttcgat tgcttctgga aaatcttctt tcttaccaga aggatcaccc
     9241 attacaatac atttattatt gaagcaagcg aattggagaa gaacagttgg ttcaccgtct
     9301 ttttcatagg caaacatccg cttatctttt aagaagatca attgactatc ggagtttcct
     9361 ccataagttg taaggatctt cagggccttt tcttcattaa aggcttcgcc aatctgtttc
     9421 ttttctcctt gaaggaaatg aacaaataga acgatcatga aactaacagc gatgatggcc
     9481 aacaatcccg aaaaccaaat cttttcagaa ggaaataaga aaaaggaaat aaaatggtga
     9541 cgccgatgag ggaaatccgg cagattgtat accccaataa cgatgtaaag caaggacagt
     9601 gcaccaataa tgattccatc gatcgtacgc cactcccatg aatagatcaa ttgttcccga
     9661 aataattcgt ttttagaagc aagaatgatc agaagcagaa tagacaagaa aaccaccggt
     9721 gtgaaactaa aatcacttaa taaaacataa agtaaagcta gaacaatcaa gaagattgta
     9781 ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc ccataatgat taaaagaaat
     9841 cccaatagta tactaggaaa ttggataata aaatgaaatt tcaatgggtt caaactatga
     9901 agccagcgaa actctgtaaa agcttgcggg atagtggcag aaagtactag catgattcca
     9961 gaaaagtata ataagacaac cacgatttta tgcgcaattt ctgttgctaa ttgtttaggg
    10021 ataccagaat aacgctgatc aaaagtggaa ccgatatttt tgaagaaaaa gacgattcca
    10081 atcaaaaacg gaatgatgta gtagaacagg cgatagagca agatccacac aacgactatt
    10141 tctcttggca caccaatagc ggacaacccg ataatcatca ttacatcgaa actcccaatt
    10201 tctcctggaa tcatagacac gattcctatc actgaagcag caatgaataa tggaattgtt
    10261 tgccacagat caatccggat ttccattaaa taaccaacag aaaggaaact aaacaggaca
    10321 ccggtccatt ccaacaatga aactagcaat agctgtcctt gagttttaag tgaggtacca
    10381 ccaaggaatc ctttcttttt gaaagtagta aataaaaaga cgacaggaaa atatagaccg
    10441 ccgccgatca accatatcca atattgcttc aaaaaaggag caactggcgt gaagactaca
    10501 agtaaaaaag aaagcagact gtaaatagaa agtccagcca tcaaaaacaa gaaaactttc
    10561 gataatgctt ggatcacgtt tctgctctct ttcttttgtc catatagttc agagcgaagc
    10621 cccacactaa caaacccgcc gaaaccggca atattgttga tcgtattgat cagccagctt
    10681 gtctcaaaca ggtaacgagg attctgcttt tgttcaagca tccgattcaa aataatatca
    10741 taaccaacca taggtaacac agagataaga ccaatcaata gcattagtcc gcttttccat
    10801 acaggtatag tggcaaacgt ttctcctaat tgctggatag atagagtttt accgatggac
    10861 atcaattcac cgacaataat gacaatgaca gaaatcaaaa agattgtttt gaacagtcct
    10921 aggtggtttt tcagccattg tatggtattt tttaacaaat aaatcacctt ctattcctaa
    10981 tagttatgtt tcagaaagaa attgaagaac ttctcgattg aagcgttcag gatcttttct
    11041 agctaattca tgtccttgtt ctttcacaag cacaaaggaa gcttttggta tagttttagc
    11101 tatatacaaa gaatgtttta gcttgattac atcttttttt cccacgatga tcaaagtggg
    11161 ggagttgatt ttttttaaat cattttctgt aagcccgatg tcatgcagca aaagtttgat
    11221 aactaaaaga tttttgcgaa gacttgggcg aaataaagag agtaaccata cccatgcata
    11281 atgaatatta ctgatgactc tggcagaaaa acgaacgcct ttaacaagcg tgtttccaga
    11341 atttaaaatc aatcgatgaa cttttttagg gaagctgctg gcaaatacca gtgctagatt
    11401 tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt ttttccagca acatgatcgt
    11461 attcagatcc tcggccatta actgaaaatt caacatagat gcttcgtttg tcgaacgacc
    11521 atgtcctcgg ctatctacta gatacacggt atagtagcgt tcgagtacag ggacctgttc
    11581 tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa aagagaggga agccttgacc
    11641 gctcttttca tagtaaattt cgctcccatc tggcattttt gcatatttct tttctttttt
    11701 cacataatca cctactgtgt aaagtataac gattatatca gtcattgggc aattgttttt
    11761 actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat acaggaaaat tcattttttc
    11821 ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa gataaagaaa tagtaagata
    11881 aaaaaagatg tcaaaataac taggtgatga aatgaagcga aaacgattat ctattatccg
    11941 tattcttttt tacatgatca ttctgacggt tattggtgcg gtgttgtttt tatgaggaag
    12001 aacttatcgt tcggtaaaac gagtggaaag ctatcaaata gaaataacaa cagctgcaaa
    12061 aaattatcat atggaaaact acgagaattt gatcaaggcg ataatattga ccgaatcaaa
    12121 aggaaaaggg acagatctca tgcagagctc ggaaagtctg catggagaag ctgagatcat
    12181 tgataatcca gctgacagta ttgatcaagg tgtcaaatat ttggcggaaa tgatagtaga
    12241 agcaaagaaa caaggatgcg atttatggac agctgtccaa gcatataatt ttgggaaaga
    12301 ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca gtagaattag cagaagcgta
    12361 ttctaaagat gtgctgagcc cacttttagg aaatgaggaa gggaaacaat accgatattg
    12421 gcggatccag tccttgcttt acaatggcgg ctatctctat cataatggag gtaacatgtt
    12481 ttatcctgat attgtcaaaa tgaatgaaaa aataatagag acctatgaaa aagtctttaa
    12541 gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa tgaaagaagg agtgcgcaaa
    12601 tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg gtacacaacc aaagactgga
    12661 tgttacggtt tgtgaaggga atgtttatcg gttcaggatt tatcttgcca ggagtcagcg
    12721 gtggtgcatt agcagctgtc tttggcatgt atgagcggct gatttctttt ttagcccacc
    12781 ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc tgttggtcta ggcggagtga
    12841 caggcatctt tctactttct tttttagtca gttttttatt aggtgcgtat gaaacaacga
    12901 tattatggtt ttttgtcgga tgtatcgctg gaactgttcc tgcgttatgg aaagaagcag
    12961 ggaaaaaagg aagagatact tctgatgtga tcatcatggt tgttactttt gttctggctt
    13021 atttgttttt attatacggc gctcaattat ttgacgggca agtcgatcag aattttggaa
    13081 catggctgat tgccggcgga ttgattggtt tgggtatgat cgtcccagga ttgagccctt
    13141 ctaatttttt ggtgtatatg ggattgtata aagcgatggc tgacggattt aaagaagtac
    13201 gacttgaagt gattgtgccg atcggaattg gtgggattct ttgtgttctt ggattgtcaa
    13261 aaatcatgga ttacattttc tcaaaggcat atagcaagct attccatttc attttaggtg
    13321 tagtttttgc ttcaacgatt atgatcattc ctaccgatta cgcgggattg ggagttgctg
    13381 gggtactagc ttgtcctgtt ctttttatag ccggtacagc actaggttgg tggatgagcc
    13441 gtttggagga acaatataag taaagataga ttgataataa aaacagcaca cgggtgattc
    13501 aaacaaaaag gttggcatct tttttgtaaa atcattcgtg tgtttttcta taaattttat
    13561 aaaaacaagt tgtttttaat cttggaacac gctagactta aacacgtgat tatttttgaa
    13621 agttggcgga aaatggagaa aaagaaaatg aatggactta cacagcaaga ggtagttcag
    13681 aaaatggctt ctggccagca aaatgactac caggaagatg catcaaaaag tacaaaacaa
    13741 atattcagcg ataatatatt gacgttattc aattttctga actttggtat tgggatttgt
    13801 ctgttatttg taggtgcgta ttccaacatg gcgtatttag caattattgt cgtcaatatc
    13861 gttattggaa tttatcaaga aatacatgca agaaatcttg tacggaaatt atcgattgtt
    13921 tcccaatcag aagtactagt gatccgggac ggtaaagaac aatcgatttc tactaaagag
    13981 ctggtattag gagatttggt aaaaatcgga gcaggagaac agataccagc cgatttacag
    14041 gttatatctg gacgggcaga agcaaatgaa gctcttttga caggcgaatc tgatttgatt
    14101 gtcaaagaaa ctggagctga attgctttct ggaagctttt tgacaagcgg ttctgtcatg
    14161 gctgaagtta tccgagtagg tgcagataat tatgctgtcc gcttgactag cgaggcaaag
    14221 actcacaaag ctattcgttc tgaacttgta gattcgattc gcaaagtatc caaattcact
    14281 agtttcgtga ttattccttt ggggatcata ctatttttgg aagcactgtt catccgcgat
    14341 gcttctatca aaatttctgt tattgcatct gcagcagctt tgttaggcat gcttccaaag
    14401 ggtttggtat tgctaataag tattgcatta acgacagggg taaccaaact ggcaaaaaaa
    14461 cgaatccttg ttcaagacat gtattcaatt gaaacgctgg cccatgtcga tacattatgc
    14521 ttggataaaa cagggaccat tactgaaggg aaaatgagta tccagcgggt agaagcacta
    14581 catccgctgt acgagaaagc catcccagaa attatgggga cgtatctggc agaaagcagt
    14641 gataacaaca tcactatgcg ggctatgaga gattattttg agctttctaa tcggtatgat
    14701 gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg gcgcgatgga atttccagaa
    14761 ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg aagaagatcg gcttccggac
    14821 agtctaaaaa cagcccaagc gaatggctat cgcgtactta tgttggggat cgctccaaat
    14881 cagcaattga acgaagaaaa accagcattt gttgagccgg ttgctctgtt tgaaatcgat
    14941 gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc ttcatgagga aggtgtggat
    15001 ctgaaagtga tttctggaga caatcctttt actgtctcca atatcgctag acgtgcagga
    15061 ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta ctgaagaaat ggaagtgcga
    15121 gaagctgctc atcgctatac ggtttttgga cgagtgagtc cgcagcaaaa gaaattgctg
    15181 gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga caggcgacgg tgtgaatgat
    15241 gttttggcgt taagagaagc agattgcagc attgctatgg cagaaggtga tggagcaaca
    15301 cgtcagattg cgaatctggt attgttggac tctgatttta ctacattgcc agaagtcctg
    15361 tttgaaggac gccgagtggt caacaatgtg acgaaagtct caggcatctt ttttatcaaa
    15421 acgatttatt catttatttt atcgattata tgtgccgtta cagcaattgg atttccgttt
    15481 atccctatcc aaatcacgct gattgattta gcgattgaag ggtatccttc cttcttttta
    15541 tcttttgaac aagataaaag aaaaatcact taccgttatt tgcctacagc acttattaat
    15601 gctttaccaa atgccttgct tgtagtagtg aatatcattg cggtgtatct acttggaaaa
    15661 acattcgcat ggggaaatgc tgaaactact actttgatgt attacttgct gattgggatc
    15721 agttgcatgg cagttgtaaa agcttgttat cccttcaacc cattgcgcat cttcctagca
    15781 gtaacgacga tcgtagggat atatgtagct gcaatgctgt tccatggaat tttggaagta
    15841 aacttagggt tgggagagag cgtaggatac ttctttgtat tcatgctcgt aaatattgtt
    15901 cttcgagtat tgtactggaa ggctgatatt caaaatcagt tactgaaaag agtagcaaat
    15961 tgatgcttaa tgagatagaa atgtgttata cttgaaaaag caatgaaata tgatacaatc
    16021 aaacatgtat aattgtgtcc tttaaaatcg cactgatttc agttagtgcg attttttacc
    16081 ggttattctc ctgaaatatc ttctgttaaa cccaaaagat atcaatagag cagggggtga
    16141 aaaaatggct caacggattc ctcaagaggt tatcgaaaca gtacgcagtc aaacgaatat
    16201 cgttgaggtg attggacaat atgtccagtt gaaaaagtct ggtaaaaatt acttggggct
    16261 gtgtcctttt catgaagaac gtacaccgtc tttttctgtt gcagaagaca agcagatttt
    16321 tcattgcttt ggttgtggta aaggcggaaa cgtgtttact tttcttcaag aactagaagg
    16381 tctaagtttt ccagaagcag tggtaaaagt ggcagacttc gaacagatac cgttagaaga
    16441 tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa tctgctacgg ggaaactgat
    16501 tgctgctcat gaaaaagcag cggaaatcta tcatcacatg ctattgcata caaaagctgg
    16561 acagccagct ttagaatatt tgcaaagtcg tgggttgtct ttagaattaa ttgaagaatt
    16621 caacataggt tttgcaccta atgagcgttc ttttcttcag caagtattca agaatgaatc
    16681 aatttcaaat gaattgttag aacgctctgg attgtttgtc acgcatgatg atggccgtat
    16741 gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt aattctcaag gccagacgat
    16801 cggtttttca ggacgctggc ttgcacctga aaaagggcag gaaaaagatc agcccaaata
    16861 cttgaatagt ccagaaacag aactgttcaa taaaagacaa gttttattta atcttgataa
    16921 agcaagaagc gatatccgga aaaacggaga aattttgttg ttcgaagggt ttatggatgt
    16981 gattgctgca tggcaggctg gtatcaaaac tggattagct tctatgggga caagtctaac
    17041 agctcaacag atccaacaaa tggaaaagct gacgaaagaa ttggtctttt gctatgacgg
    17101 agacaacgca ggttttgaag caaccaatcg agggattgaa ttgcttcggc aaaacagtcg
    17161 attagcactt tctgtcgtag tcgttccaga aaagcttgat cctgatgaat atctccgtaa
    17221 gtatggagaa gcatctttcc aagaattagt ggctcatggg cgcgaaacag tctttacttt
    17281 taaatcacgt tatttaaaac aagggaaaaa tttagataat gaaaaagaca agatcgaata
    17341 cctcgaagaa ttactagttg aacttagccg agttgtttca ccaatcgaac aagatatgta
    17401 tctatctcaa ctgtcaacag agtttcagat cggcagagag acgatacaaa aacaactgcg
    17461 cgatcttcga aggacaaatc agcaaaatcg tcaagaacct caggaaagtc aacacactca
    17521 ggttacgaga caagaaactg cacggaaaaa gagacctttg acgcaagtag aaaaggcaga
    17581 gcagattcta ttatatcgtg catttaaaga agtttctgtc cgtaatattt taaaagaacg
    17641 agaatttcag tttattcatg atgcatatgc agaagtttat tttttattcg atgcatatgt
    17701 ctcacaacat tatgaattca accttgcaga gtttttagac tttttgcaag atgaaaattt
    17761 aaggcggttg gtggtggaaa tcgaatattt accggtggca gaagaaagtt cgccacgaga
    17821 aatccaagac ttgctagatg tgatcagcaa atctggctta gctgatgaaa taacattaaa
    17881 aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa cagcttgagc ttgaattgac
    17941 gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc aaatagctac tcaagggggg
    18001 ccttctttta tggcaaatgg aacagataaa aaaacgtatg aagcagcagt agcagctttc
    18061 atcaaagaaa acaaacccaa acgacaagtt gtatatgatg atttatccaa tcagcttgct
    18121 acaccattca ctttgaatgc agatgaaatg gacaaattga tccaaaagat tgaagacgca
    18181 ggtatcagtg tggtggatga aaatggtgat ccatcaattc acagcttgaa gagtgctgag
    18241 aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc caaccggtgt aaaaatcaat
    18301 gacccggttc gtatgtatct gaaagaaatt ggccgtgtct cccttttaac tgcggaagaa
    18361 gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag aagcgaaaca gcgtctggca
    18421 gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt atgtaggacg aggcatgcag
    18481 ttccttgact tgatccaaga aggaaacatg ggactgatga aggcagttga aaaatttgac
    18541 taccgcaaag gatttaagtt ttcaacctat gctacatggt ggattcgtca agccatcact
    18601 cgagcaatcg ctgaccaagc acgtacgatc cgtattccag ttcatatggt tgaaacaatt
    18661 aataaattga tacgtatcca acgtcaattg ctgcaagacc ttggaagaga acctacgcca
    18721 gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag tccgagaaat cttgaaaatc
    18781 gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag aagatgactc tcacttaggt
    18841 gatttcatag aagaccagga agcaacaagt ccagcagaac acgcagctta tgagttacta
    18901 aaagaacaat tggaagatgt actagataca ttgactgatc gggaagagaa tgtccttcgc
    18961 ttacgttttg gtttagatga tggccggacg agaacattgg aagaagtagg gaaagtattt
    19021 ggtgttactc gggaacgtat tcgtcagatc gaggctaaag cattaagaaa attgcgccat
    19081 ccttctcgtt caaaacaatt aaaagatttc ttagaataag cgtggactat tgaccagaag
    19141 agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa aagcttgaga ctacgcagtc
    19201 tcaagctttt ttttctatag gaaggaaatt cattcattat ttttaagtaa atcctaaaaa
    19261 taagctattg gcgtgacgta tagaatatga tagattaaat ggtagcgtaa acctagtaaa
    19321 ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg gatcgaaaaa aatggagcag
    19381 aatactatct gtccagactg cggtcatcaa ataactgcag aagatcagca aataaaagaa
    19441 tcaattgaag aaacaaatac agatgcagct atgcaggata aatcaactga agatgcagtc
    19501 tttcctgata cagaattaaa cgatccaatc gaatggtcgg aattgaaaga tttacctcta
    19561 gaatctgtaa tggaattatt cgaaaaagca gaaccagaag acgaagaacc acctgctcac
    19621 gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag caactgttca tgagacttcg
    19681 gaagaatcag gaaagaatca agaagaaacg cttgaatcaa aacaaaaaag aagagtagct
    19741 gaactgaaag aaatcgtcga taatgaggaa gaaaattcga tactatccgc ttatatcaaa
    19801 gcacatcgtg aagatacaaa agaagagcac gcgaaagagc tgctcaaaat gatcagtgaa
    19861 aaaattgcac aggaaaacaa agtagagcca gaaaaggaac aagaaactga aaaagactct
    19921 gatagagaaa acgccacagg taaagaaaca gtaaaaataa aagacgtaaa aacggatatc
    19981 acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa aggaaaaaac gaataaactg
    20041 ccggaagcag agaaaaaaga aatactcgaa aacacagaca aaaagtcaac agagcccgat
    20101 gaaataaata gtgaatccga tgctgaaaat caatctgtaa gcaaagaggg tactgatctt
    20161 gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt ttgaagcaga agcgaaatca
    20221 ataacaatcg tgccagatac tgaaaaaaca catcaagaat caaacttaga aaaaacagat
    20281 ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt atctcgtttt agcagcagta
    20341 cttctactcg gtggaggaag ctgggcttat tatgaccatc atcaaaaagt agaagccgaa
    20401 gtagcagcag aagcaaaaag acagcaacaa atggaagaac ttcatgagaa acttgctgcc
    20461 ttttatacag atgatcacca tcaatttatt cgtgcagata tgattcatca agatttatca
    20521 aaactaaaaa atgatttatc cagtcttaag aatgaaaaag gatattcaga gttagaaaca
    20581 acttaccaag atatccaatc taagatccga gatatccaaa aagtgaatga gtggttcata
    20641 gcacctgcca tcgtcgatga tcatctagca gatgacatca agttgaaagc agataaagag
    20701 atccaagcaa tcgaaacgga taacacagat tttggaaaat tgattcaaca agcacaaaca
    20761 gaagctaaaa atcaatatgc tgaattgcaa acagctaaag aaaaaacagc tatagtcttt
    20821 aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat acaagacggc aaaagaagca
    20881 gtagaaaaag tgaaaaatac agatctatct gctcagttga atgaacaatt gaaacaagta
    20941 gataagatgc tcactgataa agagaaaaaa gaagaagagg aaaagaaaaa agcagaagaa
    21001 gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc aagcagcagc tgcagctcaa
    21061 gccgctcaac aagctgcggt cgcacaagca gcacagcaag aagcggcatc ctcgacccct
    21121 tcctcttcaa actcagcgaa tcagccaatc aaatctgcca gagcaagtga tattgccgat
    21181 gcatctaatc cagcatggac gtgggcacca ggtgtccaaa gcaaagtgat tgctacatgt
    21241 atccaacgtg gatatatcgt tgaaggaggt tacagattag aaaaagccag aatcgaaaat
    21301 ggggaaggct attataatct atatgccact tcaacaaaat ctgcattgat gaaaggcatt
    21361 ggagaaagtg ctttgccatt ttacatcgta accatcaact gtaaaactgg atggttcgga
    21421 ggaaatggga gcaattaatt ttaggcaaat gaaagaggct gtgagcctgc cgtttaacac
    21481 cgtttattcg aaaaagggga gtatgacaca actgttgtca tactcctttt ttcacctttc
    21541 caaaccaagg ctcagctatt caagtagata acattatgtt atgatagcaa agaaatgtat
    21601 taagaaaacc tttcactata gagggagaaa aaaatgaacg atttattagc aactatattt
    21661 acaggtatta ttattgatga aaatgaaaaa aactactttg tccaaaaaaa cggacaaacc
    21721 tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg aagcagttga aggattcggt
    21781 taccaaaatc aaaaacatga aaatcggatc actacacaaa ttcctaaaag ccgtatcggt
    21841 cattacgctt ttggaatcgt tactgctaca cgaaaagatt taggtgcgtt tgtcgatatc
    21901 ggtcttccag ataaagactt tgtcgtatct cttgatgaat tgccgacaat gacagagcta
    21961 tggcctaaaa aaggggatcg cttgatgatt tcccttagag ttgatgcaaa agaccgtatt
    22021 tggggcagtt tagcagatga aaaaatattc aaatctttaa gcaaatatgg aacagaagaa
    22081 atgaaaaaca aagatgtgat aggaacagca taccgcttga aattaacagg tacgtacgtt
    22141 ttaacggaag atttctatct tgggtttatc catccttctg aacgatataa tgaacctcgt
    22201 ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac cagacggtgt actgaatctt
    22261 tcattgaagc caagagcata tgaagctata tcagatgatg cacaaatgat tttaaccatt
    22321 ttagaacgtt ctgcagatca gcaaatcaac tttacagata aatcggatcc agaagaaatc
    22381 atgaaagctt ttggtatcag caaaggagca tttaagcgcg cattaggtaa tcttttgaaa
    22441 caaggattga ttacacaaga aaacggtgtg acaaaactag tcaaaaaatc taattgagaa
    22501 tcccttgcat gtttttctta actagcttat aataatttta attaacatag tgttatatct
    22561 ttttttattt gtaattatta taaattgttg gggttgagta aatggtcatg aatgctattt
    22621 cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg gtttaagctg acaccgcaaa
    22681 gagaagctac tctgcttgtg cttttggaaa atgagaaaga gcatttatct gcagaagaaa
    22741 tcttcttttt agtgaaaaaa aagagtccgg aaattggact tgctactgtt tatcgaaccc
    22801 ttgaaatctt gacagaattg aaaattgtcg ataaagtaag ttttaatgat ggattagccc
    22861 gttatgattt gagaaaagaa ggcgccaaac atttccatca tcatttacta tgcttgaact
    22921 gcggcacgat cgaggaagta gaagaggatc ttttacttga agtggagcaa gtagtagaag
    22981 aacgttatca ttttttagta aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat
    23041 gctatgaaaa agaaaaa
//
LOCUS       DACCIQ010000040        22992 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCIQ010000040 DACCIQ010000000
VERSION     DACCIQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.040"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(109..564)
                     /locus_tag="IVM62_001533"
     CDS             complement(109..564)
                     /locus_tag="IVM62_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845499.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IVM62_001534"
     CDS             complement(718..1926)
                     /locus_tag="IVM62_001534"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6845500.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IVM62_001535"
     CDS             complement(2038..2370)
                     /locus_tag="IVM62_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6845501.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IVM62_001536"
     CDS             2518..3393
                     /locus_tag="IVM62_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6845502.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IVM62_001537"
     CDS             complement(3475..4056)
                     /locus_tag="IVM62_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6845503.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IVM62_001538"
     CDS             complement(4062..5321)
                     /locus_tag="IVM62_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6845504.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IVM62_001539"
     CDS             complement(5323..6369)
                     /locus_tag="IVM62_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845505.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IVM62_001540"
     CDS             complement(6604..6879)
                     /locus_tag="IVM62_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6845506.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IVM62_001541"
     CDS             complement(7122..8432)
                     /locus_tag="IVM62_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6845507.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IVM62_001542"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IVM62_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6845508.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IVM62_001543"
     CDS             complement(9978..10658)
                     /locus_tag="IVM62_001543"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6845509.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IVM62_001544"
     CDS             complement(10707..11342)
                     /locus_tag="IVM62_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6845510.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IVM62_001545"
     CDS             complement(11408..12811)
                     /locus_tag="IVM62_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6845511.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IVM62_001546"
     CDS             complement(12798..13829)
                     /locus_tag="IVM62_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845512.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IVM62_001547"
     CDS             13860..14081
                     /locus_tag="IVM62_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6845513.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IVM62_001548"
     CDS             complement(14246..14983)
                     /locus_tag="IVM62_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6845514.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IVM62_001549"
     CDS             complement(15136..16476)
                     /locus_tag="IVM62_001549"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6845515.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IVM62_001550"
     CDS             complement(16713..18236)
                     /locus_tag="IVM62_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6845516.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IVM62_001551"
     CDS             complement(18614..19225)
                     /locus_tag="IVM62_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6845517.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IVM62_001552"
     CDS             complement(19555..20271)
                     /locus_tag="IVM62_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6845518.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IVM62_001553"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IVM62_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6845519.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IVM62_001554"
     CDS             complement(20830..21627)
                     /locus_tag="IVM62_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6845520.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IVM62_001555"
     CDS             complement(21597..21968)
                     /locus_tag="IVM62_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6845521.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IVM62_001556"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IVM62_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6845522.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6446 a         4913 c         3899 g         7734 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt tt
//
LOCUS       DACCIQ010000041        22787 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCIQ010000041 DACCIQ010000000
VERSION     DACCIQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.041"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            2..400
                     /locus_tag="IVM62_001557"
     CDS             2..400
                     /locus_tag="IVM62_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6845523.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IVM62_001558"
     CDS             384..1079
                     /locus_tag="IVM62_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP6845524.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="IVM62_001559"
     CDS             complement(1673..2560)
                     /locus_tag="IVM62_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP6845525.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="IVM62_001560"
     CDS             2745..3584
                     /locus_tag="IVM62_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6845526.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4754
                     /gene="sgrA"
                     /locus_tag="IVM62_001561"
     CDS             3780..4754
                     /gene="sgrA"
                     /locus_tag="IVM62_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP6845527.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5052..5408
                     /locus_tag="IVM62_001562"
     CDS             5052..5408
                     /locus_tag="IVM62_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845528.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5411..6568
                     /locus_tag="IVM62_001563"
     CDS             5411..6568
                     /locus_tag="IVM62_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845529.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6594..7034)
                     /locus_tag="IVM62_001564"
     CDS             complement(6594..7034)
                     /locus_tag="IVM62_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6845530.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7194..7700
                     /locus_tag="IVM62_001565"
     CDS             7194..7700
                     /locus_tag="IVM62_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6845531.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7868..9781
                     /locus_tag="IVM62_001566"
     CDS             7868..9781
                     /locus_tag="IVM62_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6845532.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9786..10787
                     /locus_tag="IVM62_001567"
     CDS             9786..10787
                     /locus_tag="IVM62_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6845533.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10989..11195)
                     /locus_tag="IVM62_001568"
     CDS             complement(10989..11195)
                     /locus_tag="IVM62_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP6845534.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11841..12689)
                     /locus_tag="IVM62_001569"
     CDS             complement(11841..12689)
                     /locus_tag="IVM62_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6845535.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12853..13755
                     /gene="gnd"
                     /locus_tag="IVM62_001570"
     CDS             12853..13755
                     /gene="gnd"
                     /locus_tag="IVM62_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP6845536.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13757..14758
                     /locus_tag="IVM62_001571"
     CDS             13757..14758
                     /locus_tag="IVM62_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6845537.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14755..15039
                     /locus_tag="IVM62_001572"
     CDS             14755..15039
                     /locus_tag="IVM62_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6845538.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15072..15869
                     /locus_tag="IVM62_001573"
     CDS             15072..15869
                     /locus_tag="IVM62_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6845539.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15883..16716
                     /locus_tag="IVM62_001574"
     CDS             15883..16716
                     /locus_tag="IVM62_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6845540.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16734..17591
                     /locus_tag="IVM62_001575"
     CDS             16734..17591
                     /locus_tag="IVM62_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6845541.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17613..18122
                     /locus_tag="IVM62_001576"
     CDS             17613..18122
                     /locus_tag="IVM62_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6845542.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18159..18560
                     /locus_tag="IVM62_001577"
     CDS             18159..18560
                     /locus_tag="IVM62_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845543.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18672..19025
                     /locus_tag="IVM62_001578"
     CDS             18672..19025
                     /locus_tag="IVM62_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845544.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19059..19502
                     /locus_tag="IVM62_001579"
     CDS             19059..19502
                     /locus_tag="IVM62_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6845545.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19519..20604
                     /gene="uxuA"
                     /locus_tag="IVM62_001580"
     CDS             19519..20604
                     /gene="uxuA"
                     /locus_tag="IVM62_001580"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6845546.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20597..21310
                     /locus_tag="IVM62_001581"
     CDS             20597..21310
                     /locus_tag="IVM62_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6845547.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22062..22262
                     /locus_tag="IVM62_001582"
     CDS             22062..22262
                     /locus_tag="IVM62_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6845548.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7854 a         3486 c         4715 g         6732 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca
     4381 gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga
     4441 gtagcacagg aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt
     4501 tggtacctat cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg
     4561 tgctaaaaca gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa
     4621 cagataatag aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta
     4681 catggatggg tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc
     4741 atcgagtgaa ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt
     4801 gagtgaatat ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc
     4861 tatttttgtt tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata
     4921 agagcaagaa taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac
     4981 gaatctattt gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag
     5041 gagtatgtac tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt
     5101 tgaactattg ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc
     5161 taaactacac cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa
     5221 ggtatccaga atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt
     5281 cttctcagtt tttactgagt aaagtttatt ctatactttt agagggaacg attggttata
     5341 ttttactgga ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa
     5401 ttatataagt ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa
     5461 ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat
     5521 ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt
     5581 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
     5641 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
     5701 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
     5761 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
     5821 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
     5881 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
     5941 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
     6001 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
     6061 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
     6121 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
     6181 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
     6241 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
     6301 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
     6361 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
     6421 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
     6481 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
     6541 attggaaaaa ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac
     6601 attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg
     6661 agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca
     6721 gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta
     6781 cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta
     6841 taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag
     6901 tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata
     6961 tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac
     7021 aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa
     7081 atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa
     7141 ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa
     7201 acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc
     7261 cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa
     7321 cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa
     7381 aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa
     7441 cccatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc
     7501 attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg
     7561 ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc
     7621 cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc
     7681 tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt
     7741 tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata
     7801 aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata
     7861 aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt
     7921 tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc
     7981 ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag
     8041 tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga
     8101 tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat
     8161 gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga
     8221 tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca
     8281 gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt
     8341 agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat
     8401 tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct
     8461 tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa
     8521 acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata
     8581 ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg
     8641 gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt
     8701 atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata
     8761 tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa
     8821 gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga
     8881 tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca
     8941 actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca
     9001 gttagacatc attggggatc aagagcatga agatttattt atgggccttt tgaaacaata
     9061 ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc
     9121 agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa
     9181 cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc
     9241 agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt
     9301 tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag
     9361 tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac
     9421 agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat
     9481 ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc
     9541 aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga
     9601 tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga
     9661 tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc
     9721 aggtggattt ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta
     9781 aatgaatgaa atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt
     9841 tagtcactgt atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa
     9901 aacaatttga cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa
     9961 aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata
    10021 atatggatat ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc
    10081 atggaggcgc atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat
    10141 gttcagacac gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac
    10201 atcctacacc agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag
    10261 atcaagcaga ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg
    10321 gagaatattt gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga
    10381 ctttggagaa caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg
    10441 cacttactca agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt
    10501 actttgataa taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac
    10561 atgcggatcg ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga
    10621 aacaaatgga agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag
    10681 attatgcgga gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac
    10741 aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc
    10801 aaaagaggtt gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag
    10861 cttttcatgg tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca
    10921 ccttttattc gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat
    10981 tttataagtc agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg
    11041 gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt
    11101 ccgttgttat tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta
    11161 gcgaaaaaaa gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt
    11221 gtatgataaa tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat
    11281 gatcatctag cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat
    11341 cagataggta caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat
    11401 aatagaaaag atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa
    11461 cttttttatt ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta
    11521 aaatgaggcc aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa
    11581 ttttttagaa aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact
    11641 gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct
    11701 ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg
    11761 cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt
    11821 tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg
    11881 atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa
    11941 atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa
    12001 atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata
    12061 acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat
    12121 tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc
    12181 aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga
    12241 taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat
    12301 tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca
    12361 tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt
    12421 ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc
    12481 attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag
    12541 tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga
    12601 ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt
    12661 ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc
    12721 attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa
    12781 aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
    12841 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
    12901 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
    12961 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
    13021 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
    13081 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
    13141 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
    13201 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
    13261 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
    13321 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
    13381 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
    13441 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
    13501 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
    13561 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
    13621 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
    13681 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
    13741 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
    13801 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
    13861 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
    13921 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
    13981 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
    14041 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa
    14101 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
    14161 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
    14221 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
    14281 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
    14341 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
    14401 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
    14461 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
    14521 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
    14581 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
    14641 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
    14701 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
    14761 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
    14821 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
    14881 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
    14941 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
    15001 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
    15061 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
    15121 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
    15181 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
    15241 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
    15301 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
    15361 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
    15421 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
    15481 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag
    15541 cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg
    15601 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
    15661 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
    15721 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
    15781 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
    15841 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta
    15901 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
    15961 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
    16021 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
    16081 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
    16141 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
    16201 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
    16261 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
    16321 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
    16381 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
    16441 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
    16501 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
    16561 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
    16621 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
    16681 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
    16741 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
    16801 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
    16861 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
    16921 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
    16981 gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag
    17041 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
    17101 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
    17161 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
    17221 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
    17281 gtaatgctac aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag
    17341 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
    17401 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
    17461 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
    17521 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
    17581 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
    17641 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
    17701 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
    17761 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
    17821 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa
    17881 cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg
    17941 ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag
    18001 atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag
    18061 ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat
    18121 aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc
    18181 gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac
    18241 agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca
    18301 ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag
    18361 cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga agttatttca
    18421 tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt
    18481 tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat
    18541 tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct
    18601 tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat
    18661 ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt
    18721 ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa
    18781 aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg
    18841 gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa
    18901 acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa
    18961 cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc
    19021 gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta
    19081 cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga
    19141 gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg
    19201 tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa
    19261 ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga
    19321 aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc
    19381 atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag
    19441 ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt
    19501 agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg
    19561 atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg
    19621 actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt
    19681 gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag
    19741 gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg
    19801 tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa
    19861 actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc
    19921 gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc
    19981 aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat
    20041 gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt
    20101 ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa
    20161 ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca
    20221 gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa
    20281 aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga
    20341 aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc
    20401 tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata
    20461 cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat
    20521 gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa
    20581 ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt
    20641 attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta
    20701 tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga
    20761 acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa
    20821 tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga
    20881 agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac
    20941 acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat
    21001 catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg
    21061 ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat
    21121 cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt
    21181 gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa
    21241 agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt
    21301 gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga
    21361 gggaacagat gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa
    21421 caatttagaa caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag
    21481 aagaacgaga atcgtccata tgagggtttc cactccttat gcagattttt ttaattcttg
    21541 cactgcttgt tcgatcgtaa cacctgtcgc tacatgattg gtattttctt tgtcaaaaac
    21601 gacaaataga ttcaaatctg tatccaaggt tactcgatat tgtatatctt tgttatagaa
    21661 tgtcatagcc attctccttt ctttaaatac ttgctgaaag gcaacgaacg cgttgataac
    21721 gaaattccat ttataagtat accaccaatt gaaaggtttt catagttaag aaattttaaa
    21781 gtcaaaagtt gcagataaaa gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt
    21841 attcaacttt ttcttcgatt cgaagacaat agaagacaat gaattttata tttcattgca
    21901 ttatccttgt gttattagaa aaagcatgat atattaaatg agtaatctat tttgaaacgt
    21961 aatttgagcg atatatctac atagataaaa ctccttttgc ctatcaaata tttattgcaa
    22021 cgaaaaaacg tacattgata cgtatcagga ggaatttatt tatgaataac ggtacagtaa
    22081 aatggttcaa cgcagacaaa ggttttggct ttatcactgg tgaagatgga aatgatgtat
    22141 ttgcacattt ttcagctatc caaggtgacg gcttcaaaac tttagaagaa ggccaagcag
    22201 tgtcttacga tgttgaagat ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt
    22261 aataaaaatt gacttttaac cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa
    22321 attcattggt cgttgaatgg gctgaatagt agtgtataaa acaattttgt ggagcgtgaa
    22381 gcttttatgg tagaggaaaa tacgaataaa gtgatgttga taaaaagatt cctatcttga
    22441 atatgaatcg agatgtcttg aaaatgaatg agtttgacca atcattatcc tatcgaaatt
    22501 tacagtttcg ttcgattggt ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg
    22561 aagagggcgg cacatctaaa tttttaagta acggacagtt caaatatcaa gcattgaaag
    22621 aggctatcct actgtttgaa ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag
    22681 ttcattaaat cccttcaagt gatgaaagaa tgagatctaa taaaacctga tctttcatct
    22741 gtttttttaa taacgtaata atttttttat tttttgtgct ttcgccg
//
LOCUS       DACCIQ010000042        22378 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCIQ010000042 DACCIQ010000000
VERSION     DACCIQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.042"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(5..1573)
                     /locus_tag="IVM62_001583"
     CDS             complement(5..1573)
                     /locus_tag="IVM62_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP6845549.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1577..1987)
                     /locus_tag="IVM62_001584"
     CDS             complement(1577..1987)
                     /locus_tag="IVM62_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6845550.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1984..2907)
                     /locus_tag="IVM62_001585"
     CDS             complement(1984..2907)
                     /locus_tag="IVM62_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP6845551.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2904..3782)
                     /locus_tag="IVM62_001586"
     CDS             complement(2904..3782)
                     /locus_tag="IVM62_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6845552.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3769..5106)
                     /locus_tag="IVM62_001587"
     CDS             complement(3769..5106)
                     /locus_tag="IVM62_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6845553.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5109..6212)
                     /locus_tag="IVM62_001588"
     CDS             complement(5109..6212)
                     /locus_tag="IVM62_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6845554.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6286..7794)
                     /locus_tag="IVM62_001589"
     CDS             complement(6286..7794)
                     /locus_tag="IVM62_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6845555.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7760..8452)
                     /locus_tag="IVM62_001590"
     CDS             complement(7760..8452)
                     /locus_tag="IVM62_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6845556.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8474..9448)
                     /locus_tag="IVM62_001591"
     CDS             complement(8474..9448)
                     /locus_tag="IVM62_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6845557.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9748..10620
                     /locus_tag="IVM62_001592"
     CDS             9748..10620
                     /locus_tag="IVM62_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6845558.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11078..13276)
                     /locus_tag="IVM62_001593"
     CDS             complement(11078..13276)
                     /locus_tag="IVM62_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845559.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13508..14500
                     /locus_tag="IVM62_001594"
     CDS             13508..14500
                     /locus_tag="IVM62_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6845560.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14560..15981
                     /locus_tag="IVM62_001595"
     CDS             14560..15981
                     /locus_tag="IVM62_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP6845561.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15974..17473
                     /locus_tag="IVM62_001596"
     CDS             15974..17473
                     /locus_tag="IVM62_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP6845562.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17512..18906
                     /gene="uxaC"
                     /locus_tag="IVM62_001597"
     CDS             17512..18906
                     /gene="uxaC"
                     /locus_tag="IVM62_001597"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6845563.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19065..19748
                     /locus_tag="IVM62_001598"
     CDS             19065..19748
                     /locus_tag="IVM62_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP6845564.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19750..20586
                     /locus_tag="IVM62_001599"
     CDS             19750..20586
                     /locus_tag="IVM62_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6845565.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20597..22351
                     /locus_tag="IVM62_001600"
     CDS             20597..22351
                     /locus_tag="IVM62_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845566.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         6781 a         4555 c         4000 g         7042 t
ORIGIN      
        1 taaattacag atgatctttt ccatcatact tgaaaaactc aaaatcagca taacttcctc
       61 tttttttatt tagatcatgc actgctatcc cgataaaatt tccagtaaac cctcctgaga
      121 gtatacacat gtttttttgc ggacctactt tcttccattc ttcagttgat acgtctttca
      181 aatgaaactg tgctgtctct ttctttacag tgactttcaa aaaagtcttc ttttctgtga
      241 gtggaatcgt ccaatcttct atttgtgcta ctccgttttc tgattctatc atacgtaggc
      301 attttccctt gtcctcattc caagtaacat aacaataaat ataattggat tcatttaagt
      361 acagtaccag tccagccatc tgattaaaat tattaggcat gtattcaaat gctgtctccg
      421 catcaaaagt aaaatccgtt tgccgaatag ctaacaaatg atgttcgaag gtagattgaa
      481 gagattcacc ggaaatcaga cgtaaaaaac ctggtctaca ttttgtatca caccaatcag
      541 catttggcaa aatgcgtctg gcattccagt tcttctttag ctctccttca aatgtatcca
      601 taaactgatg atctttttct tgttgtacct cttcatttgt ctcaatcatt atttcttcca
      661 tcggagttgt ctggctatct ttaagtctta accaatcgtc ggacgtccaa tacacttctt
      721 gaatagcggt ttctcttccc aaaatagccg caccgtttaa tggtctggta caaagatatg
      781 tcatgtacca tcttcctgac ggggtttgta ctaagctccc atgtcctgta cattgcaatg
      841 tagaagcagg tttgtcacta gctgtcagca taggaaaacc aggatcaagc tcgtaaggac
      901 cagtaatatt ctttgctcgg caaactgtaa ctgaatgtcc tcttcctgta ccgccttctg
      961 ccgtcatcaa ataataatac ccgttacgaa aatacaaatg tggtgcctcc gttttcgcta
     1021 attctgtacc tgcaaatatt ttatagatgg gaccatcaag cttcttcttc tctgcatcat
     1081 attcttgtaa tacgataccg ctggatttat tcggtgtgtc cagacgataa tcccatagtt
     1141 cattgacaag ccattttttt cccgtttcaa gatcatggaa caaagaagga tcaaagccgt
     1201 tactatttaa ataaattggt tcggaccaag gacctgtgat ctcagatgca gtgatcaaat
     1261 aattgtggac atctttaaac ggacggcctg cacttttgac atctgtgtaa acaagataaa
     1321 acaatccatc agcataactg atctgtggcg cccatatact tccatttgta ggatttcctt
     1381 gcaaagatac ttgagtggtc aaaatatccg ttgtatgtga ccagttgact aaatttttcg
     1441 actcatatac tcggattcct ggaagccatt caaaagagga gacaataatg taataccgct
     1501 ctttcactct taatatatta ggatctggat tgaatcctct taaaatagga tttttcactt
     1561 tgatagtctg catgattcag gctccttttc tcattcttac tcgatataaa ctgaatgccc
     1621 cagctgcgaa ataaaaaaat gcaaaaggaa acgcaataaa ctgagtgcca atcaacaata
     1681 tgaatcctgt tataaaatac aaaccagtgg cagttgttaa gtattttcct ttagcagtca
     1741 aaaacaatgc tactgctaaa aaagcaagtg aaataaacaa cacaacggca aacagaccag
     1801 tcatgtcctg taataatcca aatgtatttt ctaaagacaa agttgaatga tctacagcaa
     1861 gcgactgata acttttttta aactctgacg cagacatatt acttaaagta actgctaacc
     1921 cgccaaagaa aatcaaagat agactgccac cgaccattcc tagcaatagt tcccattttc
     1981 ttttcatttc tgaaccccct gcttttctaa atgtatccat tcaggtcttt tgatcgatgt
     2041 ttcataattg ttattacgtt cagagaattt gacagtctgt tcatttttct tgtcatccca
     2101 attatcccaa ccagttgggt gaacatgccc acctatctta cacgcttcaa ataagacatt
     2161 tgcatacgga cgccacggtc ttcctaaata gacgtttcta gtatcaggtt ctgcagtcaa
     2221 ggtacagctt tcaaaacgat accctgtttt ttgatttttc ggcgtagatg ctgctgtgat
     2281 gtatccagga tttttactgg ctaagctatg gatatgacac tgatgaaaaa cggcatctgc
     2341 cccaccaaat atgaaatcca ccgttccttc aatccagcaa tattcatagg tctgacgaca
     2401 atactcagga ttggcagaag ccggtgtaca aaaaggagta ccatcttttt gcaaatcagg
     2461 caacggaccc gtgcataagg tatcctgata accagatagt cgacagtttt ttaaagatac
     2521 gcgatctcct gaattgaata aagcaacagc ctgtcctacc ttttctcctg gacctgctgt
     2581 attttcgatg tgaagatttt ctaatacgac attcgatcct tctaagaaaa aagtagcact
     2641 tcggaaagtc cctaactggc taccgtcaag atactcctgt tttgcaaaac gattaccgga
     2701 gataactact gttcctgctc ccacaagttt aaaattggat aatctgcttt ctatatattc
     2761 atcatatctt ccttctttta ctatcaagac ctttggatgg ctatccggca ggcctgccaa
     2821 ataatcgatt ccttcttgga tagtttcaaa atccgtcggg gtatctatag cattctttcc
     2881 aattatcagt ttgtctttca tcctcataaa tgctccctta tgaattgttt ccaaatattt
     2941 ctcatttctt gtgtttccat atgaccgcct gttaaagaaa aagaagaaaa gttttctgga
     3001 ctacccattg attgatactt tttgtccagc ccttttctca gcttcagtac gccttcgtcc
     3061 aagcacatcg tatcgttttt tccaactaag ctaagacgag ggcgcggagc gattttttct
     3121 tggattgcta acgttgtata agctgtcaat agatttggca catagtaata aaacccatga
     3181 tgatctaggc ggcgatgtag caaaagtatt tcgatgtcca cctgtgccgc tagatcaata
     3241 caaactttga cacgagaatc caatgctgtc acccaccagc ttaataagcc tcccatagaa
     3301 agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc ttgtctccaa ataatcgatc
     3361 aagctgatca catcataaat acgcattccc cataacgttt ttcctgttaa taaaaattgt
     3421 ttgaacaact cgctttcaga aatacctccg cgttcttcaa atccccaagc atcaatcgac
     3481 caaacagcat atcccattcc tgtcagtact tctgcaaaag atggagaagc taagtaagga
     3541 gcaccatgga tcaattcact tttcccgact gaaaaatctc cgccgtgaga atgcaggtaa
     3601 aggaccggcg gaaacggtcc ttctttttct ggataagcat agagggctgg tactgtttcc
     3661 aattcattta agtacaattg atagtattca agcataaaac cgttttgttt ttctcggtat
     3721 aatcgttttc cactaggggt atgtctgcta gatggagcac ctaaaaaatc attcaactgc
     3781 ataagcaaag tttttacgaa caaccagttc atccatattt tctgattgat ttgattggaa
     3841 tttctcaata ctgatttctt tagtattttc taatagaaat gctggtccct cgtgattttc
     3901 aatcgacatc tggttaaagg aaaccttacc cgcaaagcca atataaaatc cttgtctgct
     3961 catttcctct attcctgtca tcatagcagg aacaccggga actgcatctt gggccatcga
     4021 gacatcgata ttgtcaaatg tgatttcttg tatgaattgt tccgctaatc cataaataaa
     4081 acctgcagaa gcatggacat tccttgccgt gatattcgaa aaatggatcc ttctgaatgc
     4141 tggtgtccgt tcatctatcg gataagcctt tttttcccat acatacggtt ctttccctcg
     4201 cggaccacag aaataataaa gattcagtat aaatggacac atcacattat ccatgactat
     4261 attggatact cgaatatctt caacgatccc gcctcgacct cgtctagatt tcaatcgtat
     4321 tcctcgatca gtttcttgga agatacaatt tgaaatggta atattacgaa tactaccact
     4381 catttcactt cctaatacga caccgccatg tccatggacc atcgtgcaat ttgtgatcgt
     4441 gatattttcg caagcgatcc tttcgtacgt atcttcagtc cctgccttga ttgcaataca
     4501 gtcatctccc acgtcaatat ggcaattact aatacgcaca tttttgcatg attctggatc
     4561 aattccgtct gtatttggtg aatccgctgg gttcaagatc gtcaagttat caaacgttgc
     4621 attactgcaa agaatcgggt tgatcgtcca acttggtgac tggatcaatt taatatcttt
     4681 cacagtaatt cgatgacagt tatggaaact catcaattta ggacgaggat aagcgagatt
     4741 atctggttca tttctaaaag tatgccacca tttttttcca tttccatcta atgtaccgaa
     4801 accagtgaca gagatgttct ctacattttg agcatagata caggaagcat aaacttttcg
     4861 atggactcct tcccaacgtg aatgaacaac aggataatct tttggatcgt cagagaattt
     4921 cagaaccgct cctgcgctta aatgaagttc gacattgctt tttaaaaata aagctcctgt
     4981 tagaaattca cctgctggaa caactacggt cccgcctccg tcacttgctg cagcatcaat
     5041 ggcttgttgt attgcttcag tattcaattc atcaattgat gcgccaaatg tcaaaatatc
     5101 atacatattt ataatgcctc cacttcaatc aatgcttgca agaatgcacc aacgcctttt
     5161 tgatcattac aaataattgg ttcactgatg tagtaagcat atgaaccatc tcgctgatct
     5221 gctccgccta accctgctac ttgacagttt ttattcaagt tcagccagcc ttcttttgtt
     5281 tccagaacaa attcatcaag taatccttga tgtgatttct tcacttgtgg aagccattct
     5341 tctttgttca gcagtcccaa acgtattcct ttagccattg cgtaaacaaa catactactg
     5401 ccggaagctt ccagataatt tcctttccgt tttgtttctg ttgttacttg ataccatgtc
     5461 ccgctgtttt cttcttgata tggaagtaat gcagccagtg tcttttctag aatttctttc
     5521 agcaaagaac aatctgtttc ttcatgaagt aattcatagg tatctgctgc agccatcaaa
     5581 taccagccaa tcgatcggct ccaaaaatga tgggatagac ctgtttttgg atcagcccat
     5641 ggttggacct tcttttcatc ccatgcatga atcagcaaat ttgtttttgg atcgaataga
     5701 tgtttataac tgatttcaaa ctgtctcaaa acatcttggt aatctttttc ttcactaaaa
     5761 ttctggataa attcagcata aaaaggagaa cccatatata agccatccaa ccaaatttga
     5821 taaggataaa tttctttatg ccaaaaagct ccttcggatg tgcgtggatg gtttgccaac
     5881 tgctgggaaa ggagttctgc tgctttttta tatttttctt cgcctgtttc tttgtacaat
     5941 aaaaagaaaa gctttcctgt atttacatgg tcgatattgt attcttcaac gctgtagcca
     6001 cggatcgttc catcttcttg cacgaaatag tcgatattgt cttttatgaa atcaaaataa
     6061 attttttctt gggttcgttc ccaaagtaat tggaaccctt taagtacgac gccatagtca
     6121 taagaccatt ttcctttata ccacttttct tcatataagc gaggtgttct ttccatgata
     6181 ctttttgcta atttctttga ccagttttcc attatttgct ctcctttaaa aattaaaata
     6241 ggatttggga caaataccaa gaagtatttg tcccaatcgt ctatattact gtgccgctgc
     6301 tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct ccttgtgctt tccagtcttc
     6361 tacagctttt ttgaatccat cttcgtcaag ctgtcccatg atgtattgca ccgtagcatc
     6421 aatgacgatt ttttcaattt ctgatcctac ttcattgttc gtcggtgaat ccaatggcac
     6481 agaaggatcc attacagcaa attcttcatt ttctttgatc aattgattag agaattcttt
     6541 ttcagggtct gtatctttga aagagaacgt cacttcacta ggacgagagc ttgataacgg
     6601 ttgcacctct tgttgccaaa gatccatatc gataaattcg atagaaccat catcattcaa
     6661 ttcataatgc gttccttcga taccgccagt catcaattta tacacatctt cttctaaaag
     6721 atcatcaaca aattgaagta atttcttcaa ttgtgcttcg tcttttactt gagatttggg
     6781 aaatgctagc aagccgccaa taccatttcc ttctgaccaa acatgataac cgccgccttc
     6841 accatctgag atcttgttta caggcactaa ttccatatca tcttgaatac cttgacccat
     6901 cgtcttcaga ttcttgatat ccaccattcc agtgtagatt ccagttcttc cttgtgcaaa
     6961 ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct tgtgccaaat agccattctc
     7021 aaatagatct cttgaaaaat tcaaagcttt catatactct tctgtatcaa attctggagt
     7081 gaatttgcca ttatcgtcta cagcccagcc atttggtgct ccaaaataag aagtgaaaag
     7141 tttaaagctg gtataacgaa tatcactagg cgcacgatcg ccaaaaccaa ctgtgtcgtc
     7201 cacaccattt ccatcaggat cgttttctgt aaattgttta gctacttcat aaagctcatc
     7261 catggtagtt ggaacatcta gtcctagatt atccaaccag tcttttcgaa tgactaagcc
     7321 agcacgagcg tagtcttttt gtatcggtac gccgtacaag gcaccgtcga tactcgcagc
     7381 ttcgattcta tcttcagaaa tttgacttaa atttttatag tcttttaaat attctgatac
     7441 atcccagaat acgcctgatt ttaacgcatt gcggacagaa gagtttttca acatcgcaat
     7501 ttgaagtgta acgatatctc ctagttcatc agaagccaat gcagttgtta atctttcttc
     7561 ttttgaagca tcaggaatcc agttgaatgt cagttccgta tcggtttttt cttcgatcaa
     7621 gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa atatttaacc aagtgatttt
     7681 agcttttcca tcttctgtct ctgaagaact gttacttgaa ctgctgccgc atgcggcaag
     7741 tgctagaatt cctgcaaaac taagacctaa ttttacgagc gttgcttttt tcataaattt
     7801 tctccttcac tgttttaata atatttttgt tttcttctgt tttttctgta tatgagttct
     7861 tccattccaa atggcaaaat atagaatttg ctgtccacgt gactgcggaa aagaagagac
     7921 tgccaccgat aaaggttaaa acacctggca ttgtttggat gatcatcaga taaatcccta
     7981 ccatacttaa tatcaacact aatgagtaat gaagatgtga gaatccgaat aaaaatgcca
     8041 ttttcatttt gaaaaaatat cctttccaat cataatgagc catgattggg aaaatataga
     8101 gtgctgagag cacatatatg aacgcagcaa tatacaaagc gatcctaaga taccaattgt
     8161 gtatgaaaga aagatccaca agtaaaacaa tcccgatggc gccatatatc cagctcatca
     8221 gcaaggtttc acgatagttt tctttaaaat atgtgaagta cgttttcgtc aaaggaaacg
     8281 tttcttttga gcgcagccat tttctaatca ctgcaaccat cgcatacgat gcaggaccga
     8341 aagtaaaaac tcccaaacct gcgatgacta aaccgcccca cagaaaattc aaataaacca
     8401 actgtaaaat acgatttagc cttccgttta ttttatctaa tgactgcagc atatctttcc
     8461 ctctctttct atcttagtaa acgccgtctt ctcctagctt tttggcaatc ttattagcta
     8521 aaattaccat aaacaatcct acgacccctt taaacaatcc cattgctgta ctgaaactta
     8581 actgtccatt cttcaatcca gctgtataaa tataggtgtc gaaaatttct gcaacgcttc
     8641 gattcaatga attcaataac aagtacatat gctcgaaccc taagtctaat gtctgaccga
     8701 tttttagaat cagcaaggta atgattactg gacgaattgc cggcaatgta acatgccatg
     8761 ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc atacaactgt gtatcgactg
     8821 cagttatcgc tgaaaggtag atgatcgtag accagcccaa ctctttccag atcacttgtc
     8881 cgatatatac tgtgcgtgtc cattcagggg atgttagaaa actgattttt tcaaatccca
     8941 ttgttgcaag gaattcattc aatacccctc cgtcaacatt caaaaataca taagtaatcg
     9001 aaacaatgat tacccaagac atgaagtgag ggatgtaaat gatcgtttga atcccgtttt
     9061 tcaatcgttt gttcgttact tcatttaata gtaaagacaa aatgattggc atcggaaaaa
     9121 acacgaagat atttaatccg aacaagatca acgtatttct taataatgta aagaatgtcg
     9181 gttctgtaaa caaacgaatg aaatgtttga aaccgaccca aggacttccc attattccta
     9241 aaaatggttg ataatcttga aaagcaataa cgagtccccc cataggaaga tacctaaaaa
     9301 tcaagaaata aaggactccc ggcagaatca tcacatataa aaatttattc gttttgattc
     9361 ctgcaattat tttttttcgt cttctgagct tttcttcttt tttcgatagc gtcagttgaa
     9421 atggttcttg tttctttaca ctttccataa gcccctcctt agttttgtga atgtttgctg
     9481 cttatgactt catgatagat aactatctga attacgtata taatcatttt taccgtaagt
     9541 aaaaacgctt acatagcaat gatttttgtg ctatacaaaa cgataaagtg tattttttca
     9601 caaataaact acgcatagca aaccttctat ttttttctat ctgacactta gcataggaaa
     9661 tgattatata cgtaatcttt caccgatgat aaattgaaca tatagaaaca gaaacattta
     9721 atttattttg aggaggagcc tatctatatg aaaaagaaag tctctatagg agaaagaagt
     9781 tttacacttt tcaacaatac gtttctaatt ttgctagctt tgatctgtat cgttcctttt
     9841 ttaaatatca tcgctacatc ttttgcttct acacaagaag tcgtcgcgaa aaagtttatc
     9901 ctttttccta ctaccttttc tttagacgcg tatcgctaca ttctttctac gccgaccatt
     9961 tttagagcac ttgctgtttc gattggtgtt acaggtgtcg gaacgatcgt cagtatgtgt
    10021 gcaacttcac tgatggccta cggtctttcc agaaagtatt tgtttggtcg aggtttcgtc
    10081 aactttcttg ttgtattttc tatgttgttc agcgggggga tgattccaac gttcttagtt
    10141 gttcgttctc tagggctggt caattcttac tgggccatga tcctgcctgt ggctgttaat
    10201 gcaatgaata tgatcattat gagaaacttt ttccaagcct tgccggatag tttggaagaa
    10261 tcagcaaaaa tggatggttg cacagatttt ggtgtatttt tcaaaattat gctgccatta
    10321 gcgttaccgt ctattgctac tatctcgctt ttttatgctg ttacttactg gaacacttat
    10381 atgactgcta ttttgtatat caacgattca tctaaatggc cgatacagat tcttttacgc
    10441 caaatcgtca ttgtatcaag tgggatgcaa gcagaaagta gtgccgttga tgtgattcca
    10501 ccagcacaaa cagttaaaat ggctgttatc gttattgcaa cagtgcctat gctgattgtt
    10561 tatccttttg ttcagaaata ttttgtcaaa ggagcactcg taggttctgt caaagggtga
    10621 ttatgtactg aacgcaaaat ggtagttgtg tcagaagtgg tcagcttcaa gaaataagaa
    10681 ggcatttacg aaaattgctt cttaaatttt tgtgaaattt cggcttattt ttcgaagaat
    10741 ctactcctgg aacaccgtgt atcagaggtt gtgaaagaga cgttcagcct tgagcgatga
    10801 taaaaactct cacaaacatt gcgacgagta acccacagga gcaataagaa gcgattttga
    10861 aaaatagctt cccatatttc acaaaattat gccttattgt cgatagcctg tctcttgaac
    10921 gccatttatt cgtaaaaaag aactgtgaca agacttttgt cacagttctt tttgcgtgct
    10981 tagccttgaa atttaactat ccataaatac aaatactagg gttattctaa cttcttttat
    11041 tgcttgaatc agtccacaag aaaaacagcc tcttgattca caaatatttt tttcgatatt
    11101 ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc ggatttcgat
    11161 attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac catttttttg
    11221 ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt tcctttttaa
    11281 atacattact taaatagtta gggttataat gcaacctatc agcgatcaca tctaaagaca
    11341 gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa tttttaaatt
    11401 catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt tctaaaacaa
    11461 gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt tgtttcatgt
    11521 tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt tcattgatca
    11581 agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa
    11641 tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct tcaaacaatc
    11701 gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca ggaacgattt
    11761 cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg gcttcttttg
    11821 cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg atgcctatac
    11881 tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa tactccatga
    11941 cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct tgcatttctt
    12001 cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg atcatgttgt
    12061 tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac agatcgattt
    12121 gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc tgcaaccgct
    12181 gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat agcgtttcca
    12241 gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct atgatccgac
    12301 tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat ttaagattca
    12361 gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag taggaaagaa
    12421 gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc agattgcgga
    12481 tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta ttataatctg
    12541 atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga ttgtaatcta
    12601 gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga gtagtaaaaa
    12661 ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca acaacttgtt
    12721 ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt tcaaacattg
    12781 gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa ttatttttgt
    12841 tatttatgta cttctgttga tacttttcaa tttcctcttc ggtcagttga ctcaacgatc
    12901 cattgattac tccccattta ccagccaaac tgactaaatc ataattatta tcatccataa
    12961 caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag taatctgtat
    13021 atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac gtagaaaacg
    13081 aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg agcaaattct
    13141 ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa atgaataaac
    13201 taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt ttataaaata
    13261 ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt tcatttttct
    13321 gtaaaattga tactcatcta attaaatgag cattccccgc tttcaagaat agcataatta
    13381 aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa gatctattga
    13441 cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa
    13501 tgatggaatg gtaacgatca aagatgtcgc taaagcagct ggcgtttctc atacgacagt
    13561 ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca
    13621 actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa
    13681 aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat caaatttcct
    13741 agcagattcc attgcaggca tccattcagt attagacaca aattacagtt tatctgttga
    13801 aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg gactgatcgt
    13861 tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa aagaatttcc
    13921 tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca ctgccgatga
    13981 tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata cgaagatcgc
    14041 ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag gggtattgaa
    14101 aagtctaaaa aaagcgagca tccctgtcaa tccagcctat tttttaacag gagattacag
    14161 cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc tgcctactct
    14221 ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac atttagctgg
    14281 cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag tcgtctccta
    14341 tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac
    14401 aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc
    14461 caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta attttatata
    14521 aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact gacaaaagaa
    14581 ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg caattttatg
    14641 cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt taaaggtaat
    14701 gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca gcaagataat
    14761 ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac ttccgagatt
    14821 atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta tctatctcta
    14881 gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg aatccaattc
    14941 gtggagactg ataatttaac agatactccc ccacagagcc tcccgggcaa actgacagcc
    15001 tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc ttgcgaactg
    15061 attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga aaaatgggat
    15121 ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg tagtttagtt
    15181 gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca acggcataac
    15241 tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat cgaagggcct
    15301 aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat catcacagat
    15361 gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca tacagcgatg
    15421 gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa agatccttta
    15481 ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct ttctttacct
    15541 aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc
    15601 catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac ccgtctgctt
    15661 ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat cactgtttca
    15721 ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga tgatgaaaag
    15781 acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc tcgattattg
    15841 caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac agatacagtt
    15901 caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa
    15961 ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc gacgagacag
    16021 tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg atcagcaaac
    16081 gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg atttgaaagc
    16141 tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag acgtaactgc
    16201 cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat tggattatcg
    16261 atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat ttcaaggtta
    16321 cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac ctaccgttgg
    16381 gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac atccagatct
    16441 gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc agcttggaaa
    16501 agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca atgccggagg
    16561 agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca aaaaaatttt
    16621 aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt acgatgagat
    16681 cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg accagcgcat
    16741 atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc
    16801 gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg ctcaaggagg
    16861 cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat tgatggcacg
    16921 agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact tcaaacagta
    16981 tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata aagcaggtgg
    17041 gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt
    17101 cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc
    17161 tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc agttggtctt
    17221 gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca aggtatctac
    17281 gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag gcgtcttatt
    17341 agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag
    17401 tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt ttaaaaacgg
    17461 agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt tatgttttta
    17521 tctgatgatt ttttattaaa agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa
    17581 aacatgccca ttattgatta tcactgtcac ttagatccaa aagaaattta cgaaaacaac
    17641 aatttcagca atctgacaga agcttggtta ggaggcgatc attacaaatg gcgcttgatg
    17701 cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg cttctgattt tgaaaaattt
    17761 ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc cgctttacag ttggtcacat
    17821 cttgaattga agcgtttctt cggtattgac ttgttgataa atgaagaaaa tgcagagaaa
    17881 atttggacac aagcaaatga aattttggca gctcctgatt ttcgccgtcg ggaaatcgca
    17941 aaaaaatcga atgtagaagt gatttgtaca acagatgatc ccgcagacga cttgcactat
    18001 catcagttgc ttgctcatga agaaccagat ttgaaggttc taccttcatt ccgtccagat
    18061 aaagcactca atattgaaaa agaaggattt aatgcatggg tcacactact ggaagaagca
    18121 gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag tcttagggca gcggatagaa
    18181 tacttccatc aacatggatg tcgtgtttcc gaccacgctt tagacatctt acgctataaa
    18241 gaagcggatg aaacagtatt agaagagatt tttcaaaaag cacgagctaa caaactatta
    18301 tcttcagatg aaatcgatgc ttatcgtaca gaaacactga tacatttgac ccacttctat
    18361 cattctcata attggacgat gcagctgcac atccatgctt atcgtaactg taatacacaa
    18421 atgttggaaa aattagggcc cgataccggc tatgatggaa tgaatgatct ttctttgact
    18481 attcctctgc aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc
    18541 ctttattcat tgaatccgaa tgactatcct gcgattttag ctttgatggg ctgtttccaa
    18601 aaagaacaga atggaaaact gcagttaggt tctggatggt ggtacaacga tactcgagct
    18661 ggtatgcgcg atcaattgac acaatttgct gatggtagtg ctttggggaa ttttgtcggc
    18721 atgctgacag attcaagaag tttcctgtcc tacactagac atgaatattt ccgtcgtgtt
    18781 ctttgtgaat ttttaggcga aatggtcatg cgaggagaag cacctgaaga tgaacaactt
    18841 ttaggaagtc tagttcaagc gattagttat acaaatgcta aaaattactt tggctttttt
    18901 gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc
    18961 cgtttatcca cgttttagag aagaagacgg accaaatttg ctccgtcttc ttctcttgct
    19021 gttctgcttc attgcctata aacctttatg aaagaagaga taacatgcct actatccata
    19081 ttgccggtga ttctactgcg gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg
    19141 gagaaaaaat ctctgcttat ttttcacccg agatccagat caataaccaa gcaaagaatg
    19201 gacgcagcac caaatcattt atccaggaag gaaggttagc ccgtctagct tcacagtttc
    19261 agccgaatga tttcttattt gtacaatttg gacataacga ccagaaaatc caagaagaaa
    19321 gaggtacgct tcccttcggt agctatttaa aaaatctgca gatatttttt gaatgcgcaa
    19381 aaaaagcgaa cgtccaaatg gtcttactga catctgtgac gcgacgagat tacttagaaa
    19441 acggaacatt gaatccagat atacttggag actatccaaa agcgatgcgt gcatttgctg
    19501 agaaacacca tattcctctt ttagatgtgt tctcaagatc acaagaattg ttccagacct
    19561 tctcaaaaga agagacaaaa aaattctacc tgcatcttat acctgatact tgtaaaaatt
    19621 acccagaagg actaaaagac aatacccact ttagtccaga aggcgctgat aaagttgccc
    19681 gaataattgt tgaattgata aaagaatccc gtctgcctct tgctaattat ttacaaaaag
    19741 gagtttgaga tgtttccatt aaatttaggc atccgtgccc acgatttaga tgttcgaaat
    19801 cgtgaagaaa tcgttcaaaa agtatcacag cttcacttgt cccatatcca atttgcacca
    19861 caaaaagcat ttcctgaaca acttaaatta actgacatga cacttgggag tgctgcctat
    19921 tttggtgaat acttccaaaa aaacaatatc gaactttcca ttttaggatg ctatatcaac
    19981 ctatccagca aaaatcaaga agttcgtgca caagcattgg caactttcaa acagcaaatt
    20041 gctctgtgct ctgcctatca agccaagatg attgctacgg aaaccggtag tgtctctgtc
    20101 ggttacacaa aagaaaattt tactgaagaa gcttatcaga tcgccagaaa ttctatcatt
    20161 gaattagtcg ctcatgcaga aaattttggc gtaactgttg ctgttgaagc agggatcaat
    20221 catccaatct ataactatca gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat
    20281 ttaaaaatca ttttagattg tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa
    20341 aaagtgatcc gaggtgcctt ggacaattta catggttata ttacagcgct tcatctcaaa
    20401 gactatatca tggaaaatgg aaagatccgc atggtacctg ttggcaaggg ctgtttagat
    20461 ttccgcccgg tactgcatta tatcaaatat gagcgtcctc taatgtacgc cactcttgaa
    20521 gctactcctg agatttatgt tcctgaagca atagaacact tgcagtttat ttaccaagat
    20581 tgttaaggag aagattatgg aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa
    20641 tgcttttcag acattgctgc atctttatca gggaaattat tggcgttttg ccatgtcagg
    20701 actctttttt attatcaaac atcttccatc ttggttaatg ccgatcgcta ttgccaatgt
    20761 aatcaatgca gcttcatcga aagattcttc tcagttgcag gccatttatt tcaacgcttt
    20821 tttgatggct tgtctgattg gtcaaaatat attgacgaat tattttcatg taaaatacca
    20881 tagcacttcg atccgtcaag tagaagctgg actaagagcg acccttatca agaaaatcca
    20941 agaactatct attccttatc aaaaagaatt gcaatctgga cgaatccagt caaagatcat
    21001 ccgtgatgta gaagctatcc aaacgttgtc ctctcaagta tttgtcagcg gattaaatat
    21061 cgctgtcaat ttattcgtag ctttaggtat cacgctttat aaaagtcctg ttgttttcat
    21121 gtttttctta ttgactgttc ctctcgccgc attcgttact ctctttttcc gtaaaaggat
    21181 cagacagtcc aactcagact tcaggcaaga aatggaagag acttctgcaa aagtgatcga
    21241 agtgatcgaa ttagcaccga ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg
    21301 cttaaataag cattttactc aaatctatga aaaaggattc cacttagata tcctgcaatc
    21361 tgttttcgga tcagtcagct gggctagttt tcagatattt cagctgattt gtttgatttt
    21421 cactgggata ttagctttac aagggcgcat ccaacccggc gatgtcgtga tgtatcagac
    21481 gtatttcacg acgatcgtca attccgtttc tggaatgatc acgctgattc ctacgatttc
    21541 aaaaggcatg gaatctatca cttctattgg cgaactgttg attgctggcg atgtagaaac
    21601 ttatcacggt aagaaaaaaa tccaacattt ggatggaaat tttcgttttg aggatgtttc
    21661 ttttctttat ccggactcac tcaaaccgat tatcgatcat ttttctctag aggtgaaagc
    21721 tggagaaaca attgcttttg ttgggccatc tggctctgga aaatctacac tccttaactt
    21781 attgattgga tttatccagc cgacttcagg aaaaattttt ctagatgatc agcctttgga
    21841 tgaactggat atgcgaagtg tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt
    21901 gttctccgct tcgatcaaag agaacatcac ttatggcttg aaaaacgtat caaaagaacg
    21961 tttagatgaa gtcatcgaag ctgcgcaact ttcttcactg attgaccagc ttcctgatgg
    22021 tctggatacc ttagtaggcg aacatggcaa caagctgtct ggtggccaaa agcaacgtat
    22081 ctccattgca cgagcgttga tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc
    22141 agctttagat aaccagtcag agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac
    22201 acccactact tttattgtgg ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt
    22261 tgtgatcgat gaaggaaaaa tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa
    22321 aggctatttc taccgcttga aaaatgaatg acaaaaaata ggagctatga caagactt
//
LOCUS       DACCIQ010000043        21495 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCIQ010000043 DACCIQ010000000
VERSION     DACCIQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.043"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            547..753
                     /locus_tag="IVM62_001601"
     CDS             547..753
                     /locus_tag="IVM62_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845567.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            949..1116
                     /locus_tag="IVM62_001602"
     CDS             949..1116
                     /locus_tag="IVM62_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6845568.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1142..1486
                     /locus_tag="IVM62_001603"
     CDS             1142..1486
                     /locus_tag="IVM62_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6845569.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1491..1772
                     /locus_tag="IVM62_001604"
     CDS             1491..1772
                     /locus_tag="IVM62_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845570.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1875..2189
                     /locus_tag="IVM62_001605"
     CDS             1875..2189
                     /locus_tag="IVM62_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP6845571.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2167..3861
                     /locus_tag="IVM62_001606"
     CDS             2167..3861
                     /locus_tag="IVM62_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6845572.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3881..5059
                     /locus_tag="IVM62_001607"
     CDS             3881..5059
                     /locus_tag="IVM62_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6845573.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5022..5708
                     /locus_tag="IVM62_001608"
     CDS             5022..5708
                     /locus_tag="IVM62_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP6845574.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5708..6868
                     /locus_tag="IVM62_001609"
     CDS             5708..6868
                     /locus_tag="IVM62_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6845575.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6878..7753
                     /locus_tag="IVM62_001610"
     CDS             6878..7753
                     /locus_tag="IVM62_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845576.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7750..8061
                     /locus_tag="IVM62_001611"
     CDS             7750..8061
                     /locus_tag="IVM62_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845577.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8051..8404
                     /locus_tag="IVM62_001612"
     CDS             8051..8404
                     /locus_tag="IVM62_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6845578.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8394..8795
                     /locus_tag="IVM62_001613"
     CDS             8394..8795
                     /locus_tag="IVM62_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845579.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8788..9192
                     /locus_tag="IVM62_001614"
     CDS             8788..9192
                     /locus_tag="IVM62_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845580.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9204..9809
                     /locus_tag="IVM62_001615"
     CDS             9204..9809
                     /locus_tag="IVM62_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6845581.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9829..10191
                     /locus_tag="IVM62_001616"
     CDS             9829..10191
                     /locus_tag="IVM62_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845582.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10194..10376
                     /locus_tag="IVM62_001617"
     CDS             10194..10376
                     /locus_tag="IVM62_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845583.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10393..13824
                     /locus_tag="IVM62_001618"
     CDS             10393..13824
                     /locus_tag="IVM62_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6845584.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13875..14612
                     /locus_tag="IVM62_001619"
     CDS             13875..14612
                     /locus_tag="IVM62_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6845585.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14622..16913
                     /locus_tag="IVM62_001620"
     CDS             14622..16913
                     /locus_tag="IVM62_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845586.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16937..19063
                     /locus_tag="IVM62_001621"
     CDS             16937..19063
                     /locus_tag="IVM62_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845587.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19226..19672
                     /locus_tag="IVM62_001622"
     CDS             19226..19672
                     /locus_tag="IVM62_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845588.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19674..19811
                     /locus_tag="IVM62_001623"
     CDS             19674..19811
                     /locus_tag="IVM62_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6845589.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19849..20142
                     /locus_tag="IVM62_001624"
     CDS             19849..20142
                     /locus_tag="IVM62_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845590.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            20139..20363
                     /locus_tag="IVM62_001625"
     CDS             20139..20363
                     /locus_tag="IVM62_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6845591.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20360..21385
                     /locus_tag="IVM62_001626"
     CDS             20360..21385
                     /locus_tag="IVM62_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6845592.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         7576 a         3255 c         4486 g         6178 t
ORIGIN      
        1 atatgcccca aattctttta ttatttcaaa tactttatca tacttttgcc ctgggttatt
       61 aagatcataa ctaatcatgt agggtttata catgttgtct tgcacatcct tgttttatat
      121 ttcagcggac cactcgctga taaataaaat tatacgctta gtatttattt tcacaatatt
      181 aatttatcgt aataactttt gagtaaatag aacaaaaaac ctgcactagt ttccgctagt
      241 acaggcagtg actatatcgg ttgataatct agcatatatt aaaaataatt gcaagaaaag
      301 aaatttatag tggtttatac caaattatca aagcaatgtt attttgttgc tgtctattgt
      361 tttttaatta ttcacacgat aactaaaggt gggtgaagag aaatgattcc attaataatt
      421 tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa
      481 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa
      541 gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagcgaca
      601 ttaaatgcct atgacggtta cgactaccga aaaaagatcg ttaagaagct atacggtgct
      661 acgtacggca atgtagtaag tgtgctatct gtacaccacc aagcttcggt cactgtggcg
      721 gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt aagttctcgt
      781 gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg tttaaaaata
      841 taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt acatattaga
      901 tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat gtatagacca
      961 caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg cgaaaaagta
     1021 tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa caatgaagtt
     1081 attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa ggagacggaa
     1141 catgaccgag gaattctata gatggctatt acagttgaca agagaagatc gtttggttaa
     1201 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
     1261 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
     1321 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
     1381 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata aacaagagaa
     1441 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
     1501 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
     1561 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
     1621 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
     1681 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
     1741 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
     1801 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
     1861 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
     1921 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
     1981 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
     2041 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
     2101 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
     2161 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
     2221 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
     2281 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
     2341 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
     2401 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
     2461 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
     2521 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
     2581 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
     2641 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
     2701 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
     2761 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
     2821 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
     2881 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
     2941 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
     3001 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa
     3061 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
     3121 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
     3181 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
     3241 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
     3301 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
     3361 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
     3421 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
     3481 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
     3541 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
     3601 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
     3661 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
     3721 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
     3781 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
     3841 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
     3901 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
     3961 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
     4021 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
     4081 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
     4141 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
     4201 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
     4261 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
     4321 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
     4381 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
     4441 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
     4501 gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt
     4561 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
     4621 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
     4681 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
     4741 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
     4801 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
     4861 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
     4921 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
     4981 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
     5041 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
     5101 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
     5161 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
     5221 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
     5281 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
     5341 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
     5401 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
     5461 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
     5521 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
     5581 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
     5641 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
     5701 taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
     5761 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
     5821 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
     5881 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
     5941 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
     6001 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
     6061 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
     6121 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
     6181 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
     6241 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
     6301 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
     6361 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
     6421 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
     6481 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
     6541 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
     6601 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
     6661 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
     6721 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
     6781 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
     6841 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
     6901 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
     6961 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta ttttttttga
     7021 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
     7081 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
     7141 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
     7201 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
     7261 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
     7321 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
     7381 taaccgccct aaagcgaatt attggaaaaa ggtttatctg gattctaatc ggctatatcg
     7441 ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt gggcacagct
     7501 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
     7561 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
     7621 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
     7681 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
     7741 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
     7801 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
     7861 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
     7921 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
     7981 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
     8041 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
     8101 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
     8161 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
     8221 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
     8281 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
     8341 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
     8401 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
     8461 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
     8521 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
     8581 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
     8641 gagattcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
     8701 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
     8761 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
     8821 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
     8881 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
     8941 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
     9001 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
     9061 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
     9121 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
     9181 ttaaataaat aggaggaaca acaatggcta aaacaacaac agtaacaact tttgacaata
     9241 taagtattaa aagaatttct tttaatttta agaatgcaac aaatgcaatt tcaactgatt
     9301 gtaatggaca attagatggc gaaacagaaa tgcagagaat tgtaaaaaaa tgcggttcaa
     9361 cagaagtaaa atcgaaatct aaaccaatca atatgacggt aacaattact gcacatgtac
     9421 cgatggaagt ttatcgacgc ttcaatgggt tgaaacaaga tgaacgtatt aaatcgggaa
     9481 tttactctta tggtcctgat tccgtagggg aagatttctc tcttgctgca gagatcgtgg
     9541 atgatttcga agaaaaaaat aagctgattg gtatgttagc atgcacttcg aatacaggat
     9601 taacattctc tattgaaaat ggtgcggatg aagtagctgc gttagaacta gaaacaaaag
     9661 ttatgcaaga tgaatttggt aaattttatc atgaagcaat tgttgcagaa cttgaagaag
     9721 acttaacaga tcaatggatg acgaatctat ctgctgatgt gattaaaaaa acttcagttg
     9781 tgacaactac ggccactcaa tcacagtaaa aaaaacggag gtagcgaaat gaacgaagat
     9841 tactcaaaaa ttgaactaaa cgatggaaca attttgaatt tagaacctaa actgaatatc
     9901 aagaaattat tgatgatcaa tagagatttt aacacagacg agtttgcaaa aatgactgtg
     9961 ggaaaaggat ccatggatat ttctgttatt caaggtgcaa aggctgtgta tattgcttac
    10021 cgccaagcga acatgactga ttatatttca ttcgatgaat ttatcgataa atgggatttt
    10081 gatatggcta ctgccagcta tatttatcaa ttgatgatgt tcaaacaagc acgcgatgct
    10141 tatcaaaaag aatttgaaaa agcaaataag gaaaaaaagc ttcaaaagta aaaatgccaa
    10201 agctcttagt tgaaacgtgg gtcgatgtct attcgatgtt gaccgacgtt ttttctatgc
    10261 cttcagattt ggttttaagc gatatctgtt tagatgacat tttgcaaatg gcttacaaca
    10321 agagtgctta tgaaggatgg aaaaactatg caataaacca atcccagaaa aactaaagaa
    10381 aggaggtaaa aaatggctaa aaagagaaca gaagcagaag taacattcat agctaacgat
    10441 gacggattga aatctacgtt aaaagaaatc agtgctgaat taactaaaaa tagagcagaa
    10501 ttaaaactag aacaagctca attacaacag actggttctg aatcagacaa gttaggaagt
    10561 aaattatctt ctttagaaaa gcagtatgaa ttacaaagtc aaaaagttga agtaactagc
    10621 caacgtttag ccaatgccaa aaaatattat ggagaaaatt ccaccgaagt tcagaaactt
    10681 gagagagaac tgattaacca acaaacagcg caacaacgtt tgtcaaacga aattgataaa
    10741 acgagtaatg cactagctca agcaaaaggc gaaatacaga cgtacgagtc tacaatgcaa
    10801 cagttggata gtgaacaaaa aaatgttcaa gctagtgctt ctctgattga atccgaatac
    10861 aaaaaatggc aagcaactgc tggtcaatca gcttctgaat ccgagaaatt agcgaaagcc
    10921 caagaatatg tttctcaaca atctgaaaat gcagagaaaa cgatagatat cctgagacga
    10981 cagttagaag ctacacagtc tgagtttggc gctacatcca cagaagcaat gcagatggag
    11041 gcgaagctta atgatgctga acgtgaattt gaagagttag gacaagctgc taaaaatgta
    11101 gatacaacta acttggacga tatcggaagc aaaatagata tgaataatct aatggaagct
    11161 tctgacgttt taagcgacat tggcgataag cttacagaat tagggaaaca agcagtggac
    11221 tctgctaata gtgtaggtag ttcccagagt aaaatacaag ctaattttgg tttgactaaa
    11281 caagaggctg aagaattaac gaatgtagcc agagacattt attataaagg ttttggagaa
    11341 tcgttagatc agtccacaga tgcattgatt ttggtaaagc gtaatttagg cgatttaaat
    11401 aatcaagatt tacaaaatat cacggaacaa gctatggtcc tagaaaacac catgggcgct
    11461 gatatggatg aaacgttacg tggtgtaaat ggcttaatgg tcaatttcgg cttgagcgct
    11521 caagatgcaa tggatttaat ggtttcgggt actcaaaacg gtttagataa aacgcacgaa
    11581 ttaggcgaca atatggcaga atatagccaa ttatggagtc aaatgggata ttcagctgat
    11641 gaaacgttcg gaatgcttca aaatggttta gatgcgggtg cttataacct tgataaagtc
    11701 aatgacttag ttaaggaaat gggaatatcg ttaacagatg gtcgatttga gcaaaacatg
    11761 gatatgttta gtgaaagtac tagaaaagct tttgaagagt ggaaaaatgg cggaggaaca
    11821 caaaaagacg ttattaattc catgattcaa gattttagca atatggatgg tcaatacgac
    11881 caattaaata aagcttctac aatttggtct gcacttggcg aagataacgc gatgaaagtt
    11941 gtccaatctt tgactgatgt taaccataca tttgatgatg ttagtggatc tgcacaaaaa
    12001 atgaatgaag attctactac tccgttacaa gaattaaacg gaaaaatagc tgaattaaag
    12061 gattcattag ctcctatagg caacacaatc atagatgcac tcgaaccagt aattgatttt
    12121 ctaggaaaga tggctgatgc gtttaataat cttccacaac cagtacagga tttcattgtt
    12181 gctataggtg gtcttacagc agcatttggc atactgttac cggcaatact agccgtgtca
    12241 tatttatttg gtccgatgat gcttataatt ggaggaatta tagctgttat agcaggcgtt
    12301 attgtggcaa ttaagaactg gggtgcaatt actgactggt ttagtggttt atggaaaaaa
    12361 tttactgatt ggttgggtga tacttgggaa agtataaaag acggagcctc atcagtttgg
    12421 gatggggtta aagaaacctg gtctggattt gtagattggg ttcaagatat ttggcaagga
    12481 gtttctgatt ggtttggaga gctatggagc ggattagttg aaggagcttc caacatctgg
    12541 caaggagtcc aagaaacttg gcaaacattc gttgattggg tttcaaatat ttggaacgga
    12601 gtcaaagaag tatggtcgat tatttgggca gacattgtag gaattgttca aataccatgg
    12661 acattaataa cgtcattgat tcaagctggt attaatatta tcgtgggtat ttttgatgta
    12721 gctggacagt tattgggcgc agcttggcaa gctgtttgga cacctatttc tgatttcctt
    12781 aaaaatactt gggatactat gacacaatgg ataagtatcg cttggaatgg gattgtaact
    12841 acattccata ctatatttga tccagtagtg gcatggtgga atggtatatg gacagctatt
    12901 agtactacgg cttcaaatat ttggaattca attagtgcaa cagcttctag tatttggaac
    12961 agtatcaaga atacaatcac tagcttggta caagcagctg ctacagtaat tcaaaatatt
    13021 tggtcaactg tatctagttg gttaggtgga atttggaatt caatcagctc tacagcatca
    13081 aatatctgga atagcgtgac tagtagtata agcaatgcta taaacgcagc taaaagtgcc
    13141 attcaaagtg tttggaatag tatatcttcg tggattagcg gaatttggaa cggtatcaaa
    13201 aatactgctt tgaatctttg gaatggaatt acaagcacta ttagctctaa agtaaacgat
    13261 ggaaaaaatg caatttcaag cggttggtcc aatctaacag gtattgtttc cgacatattc
    13321 aataatgtta aaagtacaat tgctaacata tgggaaggca tcaaaaagac tgttagcgct
    13381 ccaattgatt ggattagaga caaaatcagt ggcatttttg ataatttgaa tatttctata
    13441 ccacatattc cgttaccgga atttatcatg gaaggcagct ttaacccgct aaaaggtcaa
    13501 atcccccatt tgcgtgtaaa atggcatgct aaaggaggta tctttactaa accaacttta
    13561 ctaggtggaa tgaacggtgt cggtgaagca ggacctgaag cagttttacc tttgaaaaga
    13621 tctgttttgc aagaaattgg tgatcgtatc ttgagtagca catcagtttc atctagggca
    13681 caaacgattc aacctgtgaa taactacgaa ttcaatttca caattgatgg taacgcagat
    13741 gaggttacta tgaagcaaac aactcaacaa atcattgata gcattacaaa aattcaaaat
    13801 gataatgctt cggcatggcg ttaaacagga gagtatttct cctgtttttt tagtattaaa
    13861 aaggatgtga aaaaatgact gattgtatac attctataat cgatggattt cctgattatt
    13921 tgcataaatt ggctttagct gaaagaccaa ccataccttc tccaaaaaga cagagagttg
    13981 aaacttctgt tttaggtagg ttaggtggct tagtacaaga ttactcgttt gaagatatgt
    14041 cgtttacatt gcactataac tatttagagg atgtggaaga ccatcaagcg ttcaagcaat
    14101 cgttttatat catgcgtcat tggttaaact atgcaaagaa attagaattc tctgacgatc
    14161 ccaacgtcta ttacgttatc cagactatcg atattgggga tgcagaaaac gatattgttg
    14221 aatggggaga gttcgatgta aatattactg cgaaaccatt cgcaagagtt caagaagatg
    14281 tacctataac cgtagataaa ccacagtcat ttaacttgct gaataatagt ttagaagaaa
    14341 gttttccaaa gattatcatc actccttcag ctacttcatg ccagttcatc ttaaatgatt
    14401 atgtgtttag tttcgaaggc ttagtagcag gaactgatgt agtcattgat agtgatttga
    14461 tgctttgcta cgaagagcaa tcggacggag atattttaga tcggtccaac aaaatgaaga
    14521 ccatgcaata tccgacattg caagtggata tcaatcattt taattgcact ggtttgagca
    14581 aaatacaaat ttatcgtaac gggttaaggt aggtgaaata gatgatcgat aatttaataa
    14641 ctatttacga taaaaatgac gcgaataatt tagctgaaca tttatatgat acgcaaggtt
    14701 taggcgcttt gtcagactgg ttaacagcta ctgttagcaa taaactaaac ggagccgaga
    14761 tatttcaggg tacttattca ataaacggaa ctaatgcaga tttgattgta gacggacgta
    14821 ttattcagtg ttatgtagat gaaaatcgag caaaacagcg tctacggatt tattatgcaa
    14881 aaacttctgt aataggcaat acgatagaag taaaagctga acctattttc aatgatataa
    14941 gaaaatcggt gttgaataaa tatgacagcg gaacagaaaa gatcactgct actcaggcat
    15001 ggcaaaacgc aaaagtttta gcgaaaccag ctatcccttc gcagttttct ttctcgtcat
    15061 tagtagatac gcttgctaat gtgaagatag aaaaggcgaa ttttttagaa ttctttggtg
    15121 gaaaagaggg atctattcta gatcgatttc atggtgagtt tctaaaagat aataacacat
    15181 tacgtcatga aaaaagtcta ggtacggatc ataaaatcaa agcgatttat actaaaaact
    15241 taactggtct tgacttagag attgatgctc aaagtgtttt agttggagtt tatccattca
    15301 ttagcagctc ttcagaagga gaagacgaga tcactctacc agaagaagtt attttcacgg
    15361 attacgtgga tgattatcct gctggatatg tttcttttgt tgattttaaa gacaaagcga
    15421 ctgatgtagc cacattaagg gaagctgcta aagactggtt gaaaacaaac atagataaac
    15481 aaaaaccaca agtgagtggt tcgattgaat tagtaccatt gaggcatcaa agaggctatg
    15541 aaaaatttgt tgatctagaa aaagtttcga tgggtgacgg agtagatgtg tatcatccac
    15601 agttaaaagt gaatatgtca gcgagaattg tggaatatac gtttaatgtt ctaaccaatt
    15661 catacgataa attagttgta ggaaacgtca aaacaaactt cttagaaaac acagagaata
    15721 atgtcagcaa tttgattaat gatgccattg atcaattgaa aaacggtggc gaaatcagtg
    15781 atttactcaa tgatattgta gatcatcaaa ctgatatgat tactggtcaa aatggtggtt
    15841 atgttttatt agatcctaaa gaagcgccta gtcgtatttt gattatggac acaccagata
    15901 agaataccgc aaggaatgtt ttacaaatca acaatgctgg tattggtttt tctaaaactg
    15961 gcattaatgg aacatatgaa acggcatgga cgttagatgg cggattcaat gcctcgttta
    16021 ttacggctgg tgaaatagta ggaattacta ttagaggtac tacattaatt agtgatggtg
    16081 ctgattatag aacaagtatt gctaatggca aaatgacttg gtactcaaaa aaagttaaca
    16141 aagatattat ggagctagaa gcacgtgatt atgtaagtgc tgatgccggt attgtatcat
    16201 acaccatgaa aactggtggt ggtttcatga ttagaaatcc acagggtaac ttggttttta
    16261 gtacgtggga taatggtaat aacagaccgt ttctatcttt tggtgcgccc aatttcaggt
    16321 atagcaatgc tagttatgta acttctggcg acggtagttc tttaagcatt aatggtagtg
    16381 cgggtaactc atgggaattt aaggtagctg gtaggactat gaaatttact agtgatggta
    16441 tgctaacgtt accaggttgt ttttttggtt catgggaaga tgggaaactt gctaggtttg
    16501 aacaatcaac ggtacaagta tataaagatt ttactgttag aggtactaaa aactcaactg
    16561 taccaacaga acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt
    16621 tcgctgatta tggggaagcc gttacaggtg acaatggtaa agttcgtgtt gatattgacc
    16681 ccatgtttgc tgaaacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac
    16741 tagttttgtg tgctgttact catgaagata ttgaccattt catcattgaa actagtaagc
    16801 caaacgtatt agttagatgg aatttagtgg cacaccgtct agggtatgaa gatattagat
    16861 taaaagagga tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag
    16921 acaaggaggt atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga
    16981 attaaactac accaaagcta ttatggctcg tcaaggtgat aaagggatta cggtgacggt
    17041 taaaccgttt ctaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg
    17101 aacaacacca tctaaccgtt acgtagataa tgttgcaact agtgtaacta gtgaagaagt
    17161 cacgttttct cttgatggca catttatgag tgaagcagga tattataaac actgctacgt
    17221 agaatataga aaagacaatc aaattttaac aacgcaagat atcatttttt tctcactagg
    17281 agtgtctgac atttcgcaag gccaagccga tgaatatgtt tcgcaattag aagagttgat
    17341 tcgaaagtac aacgaaactt ttgatgcttt tatggctgaa atcaaaggta gagtggatag
    17401 cttaaatcaa cagattactg atttaactgg tcaagctaaa acgctacaag acaagttaga
    17461 tgctctgaaa gaagaaattt ctaagttagg taacttacaa gtgatgtaca gtaacagcat
    17521 cgacttcggg gactatgatt attcggagaa cccgaattta atgccatata ttacagaacc
    17581 ttgggttgga cctttgttag gtaatggaca tacagttaaa gatagtgtta aaagggttat
    17641 aactcatact aaaactagaa cagctaattc cggcgacata ttatcgcttg gcttaggtat
    17701 tccgtgtaca gcggaagcta ataataggta tcttataacc actttacgac catctacgac
    17761 atacacatta agcgttacta tgagtgtcgg ttcagattgg actggagaga ccaatactat
    17821 aggggttagg cttagatatt taaatgaaca aggtggtata gaactgccca ttaatgcatt
    17881 aataccagca aatgtagaac gcgataaaat ggtcactcat acatttactg gtataacaaa
    17941 agataatgta actagcataa caaactgtta tgttgaaata ttctcactta atagtgaata
    18001 taaaggaacg gttagtgtca gctatgacgt taagttaaaa gcacactacc ctaacttact
    18061 agatggccct tactggttag gtaaagttcc tttaggcgag aatattgctg accctacagt
    18121 tgtctttcca cacaaaacta gtgaatatat ggtttatggt agacgcaata cagaaaacta
    18181 tatcgctgac caaacatata ccatatcaat gaaagcaact aaactaacag ttcaatcgtt
    18241 tgccgtgtat attgctgctg gccgcgttaa agtaggagac atgaagccta ctgaggggtt
    18301 agctaataca tgggagttaa cttttactgt aactaagcaa catatagata gtggggtaac
    18361 taattatctg gaaatatacc aatacccgtc agctacaaaa ggagctgttc aaattgagtg
    18421 gctcaaacta gaaaaaggca atactcgaac cccgaatatt agtgagtata aatatcgtgg
    18481 tactggtatg cgtgattcaa acaatccaaa agattatgtt tgggatctag caccagaata
    18541 tgtcgaagat aatttggcca cagatattaa aatttctgaa attactggta aagcaaacaa
    18601 ttataccgat gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt
    18661 agacaccaca gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg ctatagatga
    18721 attagacaat aagatcgatg aacgcattaa tgatacagct actaccacat taacagttac
    18781 aaacgggaat accggatcag caaagcttta tcgtgaagga aaaacagttt ctatatattt
    18841 tgtggcttta aacggaaaac gcagtggtgg aaatgattca acgatactaa caattccaga
    18901 aggctatcgg ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt
    18961 gaacagtgct cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag
    19021 ttatggatcg gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata
    19081 tgaaggcagc atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg
    19141 aagaacattt accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga
    19201 aattcaatta ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg
    19261 agagtacgca ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc
    19321 accgcttgaa ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga
    19381 tcctaaattg aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa
    19441 tctcaaagca atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc
    19501 caaactcatg ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc
    19561 ggatagttta gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga
    19621 catttcagaa attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta
    19681 gttttagcga tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg
    19741 ttttcgttcc gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga
    19801 gcgcatctta attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta
    19861 ggggaatggg gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta
    19921 ataaaaccaa ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac
    19981 aatttagatt tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat
    20041 gaagaactaa agaaacacga tgaaagatta gatacacatg cagaaaaatt ggtagaacac
    20101 acgcaacaaa ttaaaacttt atttagggag agaagaaaat gaataataaa acgttcgaag
    20161 tactaaaatg gttcgcactg gtaattattc ccgcactagc tactttcgtg gggttagttg
    20221 gtaaagcgct caattggcag tacacagata tctgtgttgt catcattact ggttttggcg
    20281 cgtttttagg gagtgtgttg ggtgtatcaa atcgaaccta caaaatgttc tcggctgaaa
    20341 gcgaagaagg aggaaacaaa tgaaaaagaa aattactatt actgcgatga gcctgttaac
    20401 ggctcttttt ttattaccca ttaatacgtt tgcttatact attaatgacg agtataattt
    20461 agcgccgaat caaggagact ccagattagc aattcctaac aaaattattt tgcacgaaac
    20521 tggaatagat gcaccagcaa gaaacgtagc cgccaacatg aaaaataatt ataacggaag
    20581 caattcttat actacagatg ttattggtga cggtgggatt gtttaccgtg tgggtgagca
    20641 aggatatgtt tcgtggggag ctggtaacgc taatccttat gcgcctgtac agattgaatt
    20701 acagcgcaca tatgataaag cattgtttga aaaaaactat cgagcttata ttgaatatac
    20761 aagagatagt gcaaaaaaat atggaattcc attgactctt gatcaaggaa cttctttatt
    20821 tacaaaagga atcatttctc atttgtgggt gacaaattat gtttggggga accacacaga
    20881 tccatatggt tacttatcgc aaatgggagt tagcaaagaa aagcttgctt atgatttagc
    20941 tcatggattt accgatgaaa atccaacaac ttctgaaaac aagcctgtca ttgatccaac
    21001 acgagctggt gcagctaatc ctacgctgac agatggaaca aatcacgccc acattgatca
    21061 gtttggggaa atcgaaaacg caaacttgca tgtggctgga tggcacattg ctaactataa
    21121 atacgagtat attttcatta tggactacaa tactgggaaa gaattagctc gagtaagagc
    21181 tgatggaatt tatagaccag atgtaaacca agcttataat acttctggaa atgttggtta
    21241 tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa tgatgcgggc
    21301 aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg acaaacgttg
    21361 gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcagt acataactat
    21421 attgacaact ataaaaatta ttcgataaaa tagtgatgtt atcgcatatc ttcactatca
    21481 cccataaata gtcac
//
LOCUS       DACCIQ010000044        19945 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCIQ010000044 DACCIQ010000000
VERSION     DACCIQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.044"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            43..1809
                     /locus_tag="IVM62_001627"
     CDS             43..1809
                     /locus_tag="IVM62_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP6845593.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            1837..3645
                     /gene="uidA"
                     /locus_tag="IVM62_001628"
     CDS             1837..3645
                     /gene="uidA"
                     /locus_tag="IVM62_001628"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP6845594.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            3817..4218
                     /locus_tag="IVM62_001629"
     CDS             3817..4218
                     /locus_tag="IVM62_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6845595.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            4212..4457
                     /locus_tag="IVM62_001630"
     CDS             4212..4457
                     /locus_tag="IVM62_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845596.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(4781..5002)
                     /locus_tag="IVM62_001631"
     CDS             complement(4781..5002)
                     /locus_tag="IVM62_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845597.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(5164..5236)
                     /locus_tag="IVM62_001632"
     tRNA            complement(5164..5236)
                     /locus_tag="IVM62_001632"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5201..5203),aa:Lys,seq:ctt)
     gene            5312..5542
                     /locus_tag="IVM62_001633"
     CDS             5312..5542
                     /locus_tag="IVM62_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845598.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            5586..6755
                     /locus_tag="IVM62_001634"
     CDS             5586..6755
                     /locus_tag="IVM62_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6845599.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            7329..7898
                     /locus_tag="IVM62_001635"
     CDS             7329..7898
                     /locus_tag="IVM62_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP6845600.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7885..8745
                     /locus_tag="IVM62_001636"
     CDS             7885..8745
                     /locus_tag="IVM62_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6845601.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8935..9639
                     /locus_tag="IVM62_001637"
     CDS             8935..9639
                     /locus_tag="IVM62_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6845602.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            9644..11479
                     /gene="walK"
                     /locus_tag="IVM62_001638"
     CDS             9644..11479
                     /gene="walK"
                     /locus_tag="IVM62_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP6845603.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            11476..12792
                     /locus_tag="IVM62_001639"
     CDS             11476..12792
                     /locus_tag="IVM62_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845604.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12793..13662
                     /locus_tag="IVM62_001640"
     CDS             12793..13662
                     /locus_tag="IVM62_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845605.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            13717..14526
                     /locus_tag="IVM62_001641"
     CDS             13717..14526
                     /locus_tag="IVM62_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6845606.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(14629..15258)
                     /locus_tag="IVM62_001642"
     CDS             complement(14629..15258)
                     /locus_tag="IVM62_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP6845607.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            15428..16720
                     /locus_tag="IVM62_001643"
     CDS             15428..16720
                     /locus_tag="IVM62_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6845608.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(16796..17458)
                     /locus_tag="IVM62_001644"
     CDS             complement(16796..17458)
                     /locus_tag="IVM62_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6845609.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            17683..17967
                     /locus_tag="IVM62_001645"
     CDS             17683..17967
                     /locus_tag="IVM62_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP6845610.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            18018..19643
                     /gene="groL"
                     /locus_tag="IVM62_001646"
     CDS             18018..19643
                     /gene="groL"
                     /locus_tag="IVM62_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP6845611.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6928 a         3130 c         4085 g         5802 t
ORIGIN      
        1 actttttgga aaaatcttac taataagtag aggagaaaga aaatgactat aaaagtagac
       61 ttacgaaaaa aaccatatca tttagatgaa gatgctattt cttggattga agagacgatt
      121 gatgctatga ctttagaaga aaaaataggt caattatttg ttaatatggg atctagtcgt
      181 gataaaaatt acttaagtag tatggtcaat cagtaccata ttggagctgt acgatataat
      241 ccaggaactg ctgatgaggt atatgatcaa aactatatcc tacaaacaga aagcaaaatt
      301 cctttattaa ttgctgctaa tacagaagca ggtggtaacg gagcttgtac agatggtacc
      361 gaagttggtg tagaagtaaa aattggtgca acaaatgatg cgaaatatgc ttatgaatta
      421 gggagagttt ctggaattga agcatcggct attggatgta actggagttt tgctcctatt
      481 gtagatatta atagaaattg gagaaatcct atcatttcta gtcgatcttt tggacaaagc
      541 cctgataaag ttttagaact tagtttagct tatatgaaag gtattcaaga gagtggaatt
      601 ttgcctgcag ctaaacattt ccctggtgac ggtattgacg aacgagatca acatctatct
      661 tttagtatta atagtttaag tacagaagaa tgggataatt cttttggaaa agtatactca
      721 ggcttaatag aagctggatt accttcaatt atggcaggtc atatccatat gccagcttat
      781 tcaaaacatt ttaatcttga tctgaaagat gaagatttac ttcctgcaac tttatcaaaa
      841 gaattaatca ctgacttact tagaggaaaa ctcgggttta atggattagt agttactgat
      901 gctagtcata tgttaggaat gactggggca atgaaacggt cagaattatt gcccacttct
      961 attgcggctg gatgtgatct attcctattt tttaatgatc cagatgaaga ctttaattat
     1021 atgatggaag gttattacaa aggaattatt tcagaagagc gtttacgtga tgcattaaga
     1081 agaattttag gcttaaaggc taaactaggt ttgcataaaa aagaaaagaa taaaattttg
     1141 ttaccgaaac aagaagctat ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa
     1201 gaggtagctg atcaagcaat tacactagtt aaagataaac aagatatttg gcctattgta
     1261 ccagaaaaat ataaaaggat tttactggta gatgtacatg gggtaaaagg tgggtttggt
     1321 gaagtagttg ctggaaatag acctcaggcc attgatttat tgaaacagat tttgtcagca
     1381 aaaggatttg aagttgaaaa atacatttca cctgaagaag aattgaaagg attatcgcaa
     1441 gaagaaaaaa ttaaaaatat agctggaata tatgctgcta agagaccaat caaagattta
     1501 actgaaaaat atgacttgat tattaatata gctaatgtta acccaggtac agttcaaaga
     1561 attgtttggc cagcaagtaa aggtactccg gatattccat tttatattca tgaaattcca
     1621 acaatttttg tatctgtaca gtgtccgttt catttagctg atgtgcctca agtaaaaact
     1681 tatatcaata cttatgatgg aaaacaagaa actttagaaa tattagtgga caaactaatg
     1741 ggcctttctg aatttaaggg aataagtcca atagatgctt attgtgggtt aaaggataca
     1801 tttatttaat atttaaatta tataggagtg atttacatgt tatatcccat tatgacagag
     1861 acgaggcaag taattgattt aagcggtctt tggagattta agttagatac aaatgatgac
     1921 ttacaagaag aacttgcttt agaaccaatg gttgaaaaag agagttatcc aatttcagtt
     1981 ccttcttctt ataatgattt atttgaaaca gagaacatta gaaatcatgt tggatgggtg
     2041 tggtatgaaa aagaagtgat tcttcctaaa aaattattag aagaaagatt agttcttcgt
     2101 tttggatctg caacacatga agcaaaagta tttttgaatg gaaaattgat taatcaacac
     2161 aaaggagggt ttacgccatt tgaatcagaa attaatcagt ttataaaaga tggaagtaac
     2221 agaattacag tggctgtaaa caatgttatt gacgatagca ctttacctgt agggattttg
     2281 aaagaaacga tacaggaaga tggagacata aaaaaagaaa atttggttaa ttttgacttt
     2341 ttcaattatg caggcattaa tagacccgtt aaattgtata caactccgaa agactatata
     2401 aaggatgtca ctattaatac aaaaataatt gattcagatg caaatgtaga atttaatatc
     2461 gatacagaat ggaccggaga aattctaata gcaatctatg acgaagaagc tcaacttata
     2521 gcagaagcta aggggaaaga aggagtgatt caacttaaga acccaacact ttgggagcct
     2581 ctaaatgctt acttatatag tatgaaagtt agtttaatta acgaaaataa agtgattgat
     2641 acgtacacag aagaatttgg cattcgtagt attcaggttt cgaatgggaa gtttttgatt
     2701 aataataaac ctttttactt taagggattt gggaagcatg aggacacgta tattaatggt
     2761 cgtggtttga atgaagcagt aaatatcaaa gattttaatt taatgaaatg gatcggtgct
     2821 aattcattta ggacttcaca ttatccttat tcagaagaaa ttatgcgctt agcagatcga
     2881 gaaggtattg tagttattga tgaagtacct gcagtaggat tacatttgaa tttcatggct
     2941 actttattag atgacaacgt agaaaaacat gatacttgga aagaaatacg gacagaaaat
     3001 gctcacaaaa tggcgattaa ggaattgata gaacgcgaca agaaccatcc gagtgtcgta
     3061 atgtggtcta ttgcaaatga accagattcg gattctgaag gcgctaaaga atattttgaa
     3121 cctttagttg aattgactaa agaacttgac cctcagaaga gacctgtaac aatcgttact
     3181 tacttaaagt caacaccaga tatttgtaaa gtaggagata ttgtcgatgt tttatgttta
     3241 aatcgctatt atggttggta tgtttcaggt ggagacctaa aacatgcaaa atatctgttg
     3301 aaaaaagagc tagatggatg gatggaaaga tgtcccgata cgccaataat gtttacagaa
     3361 tatggagcag atacagtagc tggacttcat gatacagtac caacaatgtt tacagaagag
     3421 tatcaagtgg gttattacga agcaaatcac gaagtaatag ataaatgtga caattttgtt
     3481 ggtgaacaag tgtggaattt tgcagatttt gaaacaagtc aagggatttt aagagtgcaa
     3541 ggaaataaga aaggaatctt tactagagat agaaagccta aaatgattgc tcactatcta
     3601 aagaaacgat ggtcatcaat accaaatttc agttataaaa aataatttac cttttataag
     3661 gaatataaaa aaggagtggc tgcttaattg ccaactcctt ttttttataa aaagattttc
     3721 tgtttaaaga aggatatttt tttctagttt tttcgaaaaa aaggcgcttt ctgattgttg
     3781 tattagtgga aaggggaaaa aaggagcata tttatcatgc aacaaaatta caatgagaaa
     3841 tctatacaac atcaatttga tgcctactgt aaaaaagtat tacgaaacga aactaagtct
     3901 attcgaaaac gaaacattaa cataacagat aatgaggaac cattgacgaa ttcaaatgaa
     3961 ggtaagtact cccaaactca ttttgatgaa caagatgtct atttcttatt tggaatggaa
     4021 atattaattt cagatcaaaa gttatcggca gccattgatc atttatcgga aataagaaga
     4081 aaggttatcc tactttatta ttttgcaggt tttaatgata ctgaaatcgg taaaatcctc
     4141 aatatgagta caagtggtat ctggtatcaa cgaaagaagg cagttgaaca attaaaaatg
     4201 gggtatgact tatggtagac aaccccaaaa aacggcagta tccattagtt cctctatcca
     4261 ttatccaatc tgctctagaa gaagatccaa tttcaatcaa gatgatactc aaacattatc
     4321 atagttatat agctaagtta tgtttgacaa atggatttaa tgaggcgggg caatttatta
     4381 cctatgtaga tgagtatatg ctaagacagc tagaaattaa attgatagaa gcgatcttaa
     4441 aatttaaaat taactaacta aaaagtcccc tttccactta ttctgtttaa aagaatcttc
     4501 taatagttaa aggattagta gattctttta aacagaaaac ctattgatca ttgaaattta
     4561 aatacattct atcagtacgt taacacttca aggagcatat tttagttgcg ccatgatttc
     4621 ttactgtaaa gaaggagcga taaacattct aaaataattt ttatagtcaa taaaaagcga
     4681 tgatttgaag ttgggataat gatacttccg taaggctcgc cgtaaacttg gagagaggtg
     4741 aaattcctat gtgcttttga caaaagcagc tgattaagaa ttatttccat atgttgattt
     4801 caacattttt tctattattt ttaagaccaa attcagtgag gcaaatagca tcatgtaaat
     4861 ttagtttttc tattgaacgt tctccacttt ttagtttttt tatagtactc aaatttaatc
     4921 ctgtatgttt agagatctca tttgcagtat ggttttgtaa agtttctcgg attattgtta
     4981 tattagcaat ttcattacgc ataattatgt tcctttctta tcaaaaatat caatgactac
     5041 agtgggcttt tagtgaaaaa aaggggtact acagtctctt ttgaaaacaa aaaaatacag
     5101 tatatacgtt ttctaaatgc taagcaccaa cgtttacact gtatttaagg gtttggcacc
     5161 caatgggctg tgaggggctc gaacccgcga cccgatgatt aagagtcatc tgctctacca
     5221 actgagctaa cagcccaaat taatactttg ttattctaac acctaaaata aaaaagtgca
     5281 atacttatta tcagaattga gagtgaattt tatggatttt caagattatg aaaaaatatt
     5341 tgaaagttat ccatcgatat tgaaaattac tgatttaact aaaatgttaa atattggaaa
     5401 aaataaagct tataatttgc tgaaagatag atcaatagaa aatataaaaa ttggagcaag
     5461 gtacaaaata cctaaaaaag ctgtcattga gtatatattg aaaatacaag atgaaggtaa
     5521 aaatcatgct ataataaaat agtcaaaatt tttgccgata tttcagttta agaggagaga
     5581 ttgaattgaa attaagagca aacataattg aaaaaagagg aacattacaa gtagttgtat
     5641 cttataaaga ctctaacgga aaaattagac aaaagtggaa agatacaaaa ttaaaaaaga
     5701 aaggaaataa aaaacgagca gaaaaaatta gagatgaatt tttactagaa ttggaaagtc
     5761 aattagctat ggaatgtgaa tgtgctaatc caaatttatt gttttatgac tatttgctta
     5821 attatttgga agtatcaaaa aaacaagtag catttaacac atatgttggg tataagcatt
     5881 acgtccataa tcgaatttac aagttttttt atcctaaaaa aattaaacta aataggttaa
     5941 agccttatca tttacaagag ttttatcaat atatgttaaa tgataactgt acagcaaata
     6001 cggttattca ttatcacgca tttatacgca aagctcttca agaggctgtt attacagaat
     6061 tgataagtac aaatgtagca gataaagtaa gaaagccaaa aaagaaacaa tttgtttctc
     6121 aagtttataa tcaacaagag atcttgaaat tacttgacat tttgtcagaa gaaaaactct
     6181 atttggtagt tcttttaaca gcattttatg gattgcgaag aagtgaagtt ttaggattaa
     6241 aatggtctgc tattgatttt ttaaacaaaa aaatcatgat aaaccatgta atcatagaaa
     6301 atccagagaa tatgtcacag ttaataaaga aagatcaaac aaagaactct tccagctatc
     6361 gtacattacc tttggttgaa acgattgaac gagcattagt aaatcaagct aaatggcaac
     6421 aagataatcg tattctttta ggagatgatt atattttaga agataatgag tacgtattta
     6481 ctatggaaga tggacggttg atgaagcctc aatatgtaac gcatcgtttg agtaagttga
     6541 ttaagaaaaa tggattgaaa aaaattcgct ttcatgactt gcgtcattct aatgcttcaa
     6601 taatgttaga tagtggccag aatatgaaat ccattcaaga atggctggga catgcatcgt
     6661 attcgacaac tgctaattta tacacgcatt taaccgcagg agtaaaagat cgtgcagcag
     6721 aagcattaga aaacgcattt gggtttaaag aataaaaaaa caaaaaacaa tatctttggg
     6781 gtatcgcttc ttgtttttag aatttttttg ttggcaattt gttggcagta tgttggcaat
     6841 gaaaaaattc acaaactaaa aacctattta taaagcttta taaaactttt ttcacaaata
     6901 gagcatttgg ttaagagtca gctgctctac caactgagct aacggcccgt acaaaaagta
     6961 cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag aaaatcagaa
     7021 aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct cgctattata
     7081 taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt ttttcatttt
     7141 ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa aagacaagaa
     7201 ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca cctagttaaa
     7261 gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg aaaaaaggag
     7321 cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa ctagaaacat
     7381 tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac caagtcagaa
     7441 cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca ttgcttacct
     7501 cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt aaaaaaagat
     7561 ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa gtattgacta
     7621 ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt cgtgaagcct
     7681 ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa ttagcacaag
     7741 gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt gctgtaagag
     7801 cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg
     7861 aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt agtagattca
     7921 ggtacggatg tgccagaaga attgataaaa aaatacgaga tgtatatgat cccattgaaa
     7981 attatttata aagatcaggt atatacagat aaaattgaca ttacctctga agagatctat
     8041 caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc gatcacagaa
     8101 atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac aatttccagt
     8161 ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga agaattggaa
     8221 gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc gatccaagca
     8281 gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct gaatgagtta
     8341 gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca aaaaggtgga
     8401 cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc aatcatctca
     8461 tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg
     8521 gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt caacttagct
     8581 gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact taaaagagag
     8641 ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt agtccacact
     8701 ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa
     8761 cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa aataataaag
     8821 tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg aaacaaaagc
     8881 agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct ctttatgaaa
     8941 aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt caatttagcc
     9001 aaagaaggct atgatgttta tactgcatat gatggggaag aagccttgga aaaagttgct
     9061 gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga cggcttagaa
     9121 gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac agccaaagat
     9181 tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt gaccaagcca
     9241 ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct
     9301 gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt gacgatccat
     9361 ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca tcgtgaattc
     9421 gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga acatttatta
     9481 caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt aactgtacgt
     9541 cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt gacacgtcgt
     9601 ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa gaaaagtccg
     9661 cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt tattgatctc
     9721 gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga tcaatacatt
     9781 tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc cggaattgaa
     9841 tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa
     9901 cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg
     9961 aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa
    10021 tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg tgaatatcaa
    10081 tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt acgtcaaaag
    10141 taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct ttactgcctc
    10201 actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt cgattacaca
    10261 gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact acagtcggaa
    10321 agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca atcagttagc
    10381 tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt
    10441 gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag tcattacgat
    10501 caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg gacaatcgat
    10561 tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag aagatccaaa
    10621 cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga tcttgcgagt
    10681 agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag ctgtcttgca
    10741 tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc
    10801 ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat tgagcgaagg
    10861 tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag atgaaacaga
    10921 ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata caggtaatac
    10981 ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc ttgaccggtt
    11041 tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag
    11101 agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata atatcatgaa
    11161 taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat tagaaacgca
    11221 taataacgtg gttttaagta tcaccgatca aggattgggg attcctaaaa aagatctgaa
    11281 ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac aaggtgggac
    11341 aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg ctatttgggc
    11401 agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg aaccttatga
    11461 ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact cattttgatg
    11521 gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg aaaaagttca
    11581 atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc
    11641 gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca agaaaccaac
    11701 agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt tggaaagctt
    11761 tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct gaactctggt
    11821 tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc atttgattta
    11881 gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac tagaatacaa
    11941 ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct cgtatatgaa
    12001 gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa agacacaaaa
    12061 tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac gaaacattcg
    12121 atcaaactga aaaagtatag ttatatcctg tcacagcaac cctatacact ttttagaaat
    12181 gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga attgattttt
    12241 tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga ttttcgtgga
    12301 gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc ctatattagc
    12361 aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa tacgatcgat
    12421 tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg acagattggt
    12481 gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac cagtttgaat
    12541 acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc
    12601 atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt gatcgttggc
    12661 tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc tgaatggtat
    12721 gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt acctggattg
    12781 gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg
    12841 ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa caatgtcatt
    12901 cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat cacttacacg
    12961 gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca gaccaatctt
    13021 agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg
    13081 tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga aaagaacgtg
    13141 atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa agacaatatt
    13201 ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga agaaccacta
    13261 gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac agcaaaaatc
    13321 gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat gcagacgcat
    13381 ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga ggaagcaatc
    13441 aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg gcgtcagcta
    13501 gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta
    13561 gcgatcgaaa caagcgataa agtggtccaa attgagagtg tcaacgcttt cagcaatacg
    13621 atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga gtataatgaa
    13681 aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa accagctttt
    13741 aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga aagtgaaaaa
    13801 aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa
    13861 attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca tcgagatcat
    13921 atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc caatcagcag
    13981 acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa acatctattt
    14041 gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg cgtctcccat
    14101 gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg
    14161 acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc cgatggttat
    14221 ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc gtggaattta
    14281 aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc acttgtcatg
    14341 actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag caaggaaaac
    14401 aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa agattttggc
    14461 gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga gctattttcg
    14521 atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa tcagggttgg
    14581 ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt atcgactgac
    14641 tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta atgcaatcgc
    14701 gtaatctgcc atgctgatcg tactgtcgcc agcagcgttt ctttgtaaat aatcatcact
    14761 taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg gactgatata
    14821 agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca tattgaaagc
    14881 tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg tcttttctgt
    14941 atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg ttccttcaag
    15001 catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt tgggagcgtt
    15061 gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact caaataaatc
    15121 cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga ttgccgtcac
    15181 gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc cagtagctcc
    15241 aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt aagcagttgc
    15301 tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct ttcatggttc
    15361 gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa gttgaaggag
    15421 ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat ctgttgtgaa
    15481 tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa taattattgt
    15541 tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc agtggataga
    15601 caaaacagga ttttctatgt attttacaca agtataccag gctacatttg atgtgatagc
    15661 cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt
    15721 accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac taaatactca
    15781 gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa ctgggctcat
    15841 catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc aaagagagaa
    15901 gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg ttatagtcac
    15961 ttttacgctt atcggttcag cggtttttca acagttgttc agaatgactg ttgttcaagg
    16021 agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt ttggtgtgct
    16081 tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat ctgcgatggt
    16141 caatgggttg gttagtcccg tcttgcaagc gaattcatta gcgaatgcag aaatcttagc
    16201 ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc aatttctcga
    16261 tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt ttatgacggt
    16321 ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac cagccttttt
    16381 caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga tgatggctgt
    16441 tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt tatattttgg
    16501 attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga tcctctcggg
    16561 tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt caattagttt
    16621 tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa aggaaagaca
    16681 gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc
    16741 gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt catgtttata
    16801 ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg
    16861 tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag aagaaaccta
    16921 ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc aaagagctga
    16981 tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata ctgcggcgga
    17041 atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt
    17101 ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg ccaaacaata
    17161 cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt ccaaccaatc
    17221 cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc gctaagggtt
    17281 cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac atgactgcag
    17341 ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg atcaagggtg
    17401 agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt aaagacatgt
    17461 aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat tgatttcaat
    17521 tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg atttaaaagc
    17581 ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc gaaggttaag
    17641 agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa accattaggt
    17701 gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta
    17761 gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg tgaaggccgt
    17821 ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca agttatgttc
    17881 gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat cgtcgcagga
    17941 aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc aaaaataatc
    18001 aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta
    18061 tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga cctaaaggcc
    18121 gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat ggtgtgacga
    18181 tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag
    18241 aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca actgttctga
    18301 cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat cctctaggca
    18361 ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat aatatctcaa
    18421 ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg ggttctgata
    18481 aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa
    18541 tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg cagtttgatc
    18601 gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct gttctagaaa
    18661 acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt ctgccattgt
    18721 tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat gtagatggtg
    18781 aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa
    18841 aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct attttgacag
    18901 gtggtacagt aattactgac gatctaggtt tagaattgaa agatgtaaca atcgaaaact
    18961 taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta gaaggttctg
    19021 gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa
    19081 cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca ggtggtgtag
    19141 ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa ttacgcattg
    19201 aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca ggtggtggta
    19261 cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga gacgtggcaa
    19321 caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg
    19381 ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta ggcactggtt
    19441 tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta gacccaacta
    19501 aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta ttaacgacag
    19561 aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca atggatccat
    19621 caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact gatcattagt
    19681 gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat aagaagaaat
    19741 tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttc
    19801 tgcttccgct gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
    19861 acgctctttt ttgtgtgtaa ttataatgaa aatagttatt tttaatagtt ataatatttt
    19921 atctattaaa agtaaatatc cattc
//
LOCUS       DACCIQ010000045        19818 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCIQ010000045 DACCIQ010000000
VERSION     DACCIQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.045"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            258..533
                     /locus_tag="IVM62_001647"
     CDS             258..533
                     /locus_tag="IVM62_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845612.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            546..2618
                     /locus_tag="IVM62_001648"
     CDS             546..2618
                     /locus_tag="IVM62_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6845613.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2783..4669
                     /locus_tag="IVM62_001649"
     CDS             2783..4669
                     /locus_tag="IVM62_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6845614.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IVM62_001650"
     CDS             4681..5628
                     /locus_tag="IVM62_001650"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6845615.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IVM62_001651"
     CDS             5648..6175
                     /locus_tag="IVM62_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6845616.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="IVM62_001652"
     CDS             complement(6238..6891)
                     /locus_tag="IVM62_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6845617.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="IVM62_001653"
     CDS             7023..7865
                     /locus_tag="IVM62_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6845618.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="IVM62_001654"
     CDS             8023..8919
                     /locus_tag="IVM62_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6845619.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="IVM62_001655"
     CDS             8922..9635
                     /locus_tag="IVM62_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6845620.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="IVM62_001656"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="IVM62_001656"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6845621.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="IVM62_001657"
     CDS             10170..10397
                     /locus_tag="IVM62_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6845622.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="IVM62_001658"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="IVM62_001658"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6845623.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11725)
                     /locus_tag="IVM62_001659"
     CDS             complement(11183..11725)
                     /locus_tag="IVM62_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6845624.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="IVM62_001660"
     CDS             complement(11962..12360)
                     /locus_tag="IVM62_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6845625.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="IVM62_001661"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="IVM62_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6845626.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="IVM62_001662"
     CDS             13473..14240
                     /locus_tag="IVM62_001662"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6845627.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="IVM62_001663"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="IVM62_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6845628.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="IVM62_001664"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="IVM62_001664"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6845629.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="IVM62_001665"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="IVM62_001665"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6845630.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="IVM62_001666"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="IVM62_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6845631.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6874 a         3414 c         4121 g         5409 t
ORIGIN      
        1 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
       61 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
      121 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
      181 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
      241 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
      301 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
      361 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
      421 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
      481 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
      541 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
      601 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
      661 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
      721 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
      781 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
      841 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
      901 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
      961 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     1021 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     1081 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     1141 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     1201 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     1261 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     1321 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     1381 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     1441 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     1501 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     1561 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     1621 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     1681 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     1741 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     1801 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     1861 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     1921 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     1981 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     2041 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     2101 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     2161 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     2221 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     2281 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     2341 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     2401 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     2461 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     2521 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     2581 ttgcgtttcg agcaaagtca aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcgttttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacatcg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttctggcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaaca
//
LOCUS       DACCIQ010000046        19741 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCIQ010000046 DACCIQ010000000
VERSION     DACCIQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.046"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            6..305
                     /locus_tag="IVM62_001667"
     CDS             6..305
                     /locus_tag="IVM62_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6845632.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVM62_001668"
     CDS             378..1739
                     /locus_tag="IVM62_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6845633.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVM62_001669"
     CDS             1761..2021
                     /locus_tag="IVM62_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845634.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVM62_001670"
     CDS             2035..2787
                     /locus_tag="IVM62_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP6845635.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVM62_001671"
     CDS             complement(2881..3486)
                     /locus_tag="IVM62_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6845636.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVM62_001672"
     CDS             complement(3539..4513)
                     /locus_tag="IVM62_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP6845637.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVM62_001673"
     CDS             complement(4891..5670)
                     /locus_tag="IVM62_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6845638.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVM62_001674"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVM62_001674"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6845639.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVM62_001675"
     CDS             7161..8558
                     /locus_tag="IVM62_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6845640.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVM62_001676"
     CDS             8639..9079
                     /locus_tag="IVM62_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6845641.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVM62_001677"
     CDS             9227..9553
                     /locus_tag="IVM62_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6845642.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVM62_001678"
     CDS             9554..10303
                     /locus_tag="IVM62_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845643.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVM62_001679"
     CDS             complement(10383..10838)
                     /locus_tag="IVM62_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6845644.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVM62_001680"
     CDS             complement(11265..11540)
                     /locus_tag="IVM62_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845645.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVM62_001681"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVM62_001681"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP6845646.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IVM62_001682"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IVM62_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6845647.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IVM62_001683"
     CDS             14770..17436
                     /locus_tag="IVM62_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6845648.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IVM62_001684"
     CDS             17800..18552
                     /locus_tag="IVM62_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6845649.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGGFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IVM62_001685"
     CDS             complement(18574..19728)
                     /locus_tag="IVM62_001685"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP6845650.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6287 a         3468 c         4052 g         5934 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcggatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 t
//
LOCUS       DACCIQ010000047        19568 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCIQ010000047 DACCIQ010000000
VERSION     DACCIQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.047"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            208..588
                     /locus_tag="IVM62_001686"
     CDS             208..588
                     /locus_tag="IVM62_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP6845651.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            585..854
                     /locus_tag="IVM62_001687"
     CDS             585..854
                     /locus_tag="IVM62_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845652.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            854..1774
                     /locus_tag="IVM62_001688"
     CDS             854..1774
                     /locus_tag="IVM62_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845653.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            1796..2668
                     /locus_tag="IVM62_001689"
     CDS             1796..2668
                     /locus_tag="IVM62_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6845654.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            2687..3859
                     /locus_tag="IVM62_001690"
     CDS             2687..3859
                     /locus_tag="IVM62_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6845655.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            3870..4520
                     /locus_tag="IVM62_001691"
     CDS             3870..4520
                     /locus_tag="IVM62_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845656.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            4536..4883
                     /locus_tag="IVM62_001692"
     CDS             4536..4883
                     /locus_tag="IVM62_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845657.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            4876..5874
                     /locus_tag="IVM62_001693"
     CDS             4876..5874
                     /locus_tag="IVM62_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6845658.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            5898..6182
                     /locus_tag="IVM62_001694"
     CDS             5898..6182
                     /locus_tag="IVM62_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845659.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            6263..6661
                     /locus_tag="IVM62_001695"
     CDS             6263..6661
                     /locus_tag="IVM62_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6845660.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            6671..9214
                     /locus_tag="IVM62_001696"
     CDS             6671..9214
                     /locus_tag="IVM62_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP6845661.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            9236..11578
                     /locus_tag="IVM62_001697"
     CDS             9236..11578
                     /locus_tag="IVM62_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845662.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            11594..13768
                     /locus_tag="IVM62_001698"
     CDS             11594..13768
                     /locus_tag="IVM62_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP6845663.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            13758..14180
                     /locus_tag="IVM62_001699"
     CDS             13758..14180
                     /locus_tag="IVM62_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6845664.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            15123..16034
                     /locus_tag="IVM62_001700"
     CDS             15123..16034
                     /locus_tag="IVM62_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845665.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            16521..16787
                     /locus_tag="IVM62_001701"
     CDS             16521..16787
                     /locus_tag="IVM62_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845666.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            16859..17200
                     /locus_tag="IVM62_001702"
     CDS             16859..17200
                     /locus_tag="IVM62_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845667.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            17255..17674
                     /gene="ssb"
                     /locus_tag="IVM62_001703"
     CDS             17255..17674
                     /gene="ssb"
                     /locus_tag="IVM62_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6845668.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            17751..18047
                     /locus_tag="IVM62_001704"
     CDS             17751..18047
                     /locus_tag="IVM62_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845669.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            18062..18493
                     /locus_tag="IVM62_001705"
     CDS             18062..18493
                     /locus_tag="IVM62_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845670.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            18675..18980
                     /locus_tag="IVM62_001706"
     CDS             18675..18980
                     /locus_tag="IVM62_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845671.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            19384..>19568
                     /locus_tag="IVM62_001707"
     CDS             19384..>19568
                     /locus_tag="IVM62_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6845672.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEEC"
BASE COUNT         6921 a         3053 c         4168 g         5426 t
ORIGIN      
        1 taaaaaggca aagtatgtac atggtattta tcgtgttcat actttgcctt tttatggcga
       61 aattttttag aaaggaggaa aaaattttgt tatttgtaag tggatttcta gctggtggtg
      121 tattggccac cttttttatg gcaagtttca ttgttagtaa aacagaagaa actagaatca
      181 aaaataaaca ttagaaagga caaagatatg atcacaaatt ataaaaaatt ttggcagaat
      241 agctttaatt acaaagggaa agccacaaga aaagagtatt ggctcgttgc gttaatgaat
      301 gccctcattg ggtttggcct aggcttagcg attggactag caggagcgtt gaatgcttct
      361 gaaacaataa ttggttgttt gataggcgta ttgattgtgt attgtttcgt agttcttgtc
      421 ccgtctcttt ctctcagcgt tcgacgttta cgagacgcag gttttcattg ggcatttatt
      481 tttttctatt ttgtcccaac gattggctct cttgtgttac tggttcttta ttgcttaccg
      541 acaaaagagt caatagaaga taaacaggta caggaggaaa ccagatgatt atagtaacag
      601 gaattgtttt attagggata ggcgttttat ttggctatca tttcgcctat tttccaaaga
      661 ttggcgaatg gcaacaaaga caagcgatcc aaaaatattt tcaagtcaaa ggcaacgaat
      721 atacctatta tgctgaagat gaggatagct atatcgtcag tttgaacaat caagaatatc
      781 gggtgaaatt ttctttaaat aaccctattc aagtggtgta tgcggtagaa gtggtggaag
      841 aagtgtttga ctaatgatga tgattcgatt tacagaagtt gataacatgt ctttactaac
      901 agataaaaaa attgcattag ataaggcatt tattcaattg aaaagagaaa atgaagagct
      961 attggcacga gcggaaattg gctggtgtac cttggaaatc tacacagaaa accatgaaat
     1021 tccagtattt gaaggctcgt atgattttcc agtagaagag tttcatttga tttcattgat
     1081 tgaacaatct ttaacagaaa atgggcaatt aagtgaagaa gcacaaacct ttattcaaac
     1141 cttaaattcc gaattaccga agttttatca agtaaagaaa gaccaaaaga acgaacgtcg
     1201 accaacaaaa atcaaaaagg aaaagaaaac accactttct gtagaaacag cgtttacaca
     1261 aagcaacggc tctcttcaga agaaaaaaat gtcttttcaa tggcttttgt ttggatttat
     1321 cgtcgttgta tcttgtggtc tttttattat gcaactttca acgcaaagag agattgaaca
     1381 actaacgaaa aaggagcacc agttacaaga acgcattgca tttgcggaag aacttgcgac
     1441 aaaacaacca caaattgata catttagtcg ttattttatt acgaattatt attcgggtga
     1501 aatggcagaa gaacggtata ggaaaaagct tcaatcgttt ctaacggaaa aagttgcgaa
     1561 acaaatcagt cctcaaaagg aaaaagtcaa aagtatttta tcctgggaaa tgaagaaaga
     1621 aggcaagcat tggcaaattg cgtatgtcat tacattggaa accgagaaaa agacacaaga
     1681 aacaaaaaaa gtgtcttttc aattggaaga acaggaaaaa aattatctag tttccacgct
     1741 tcccgaacta gatgattttg aaataaatca ataaataaga gaagaggaaa aacacatgaa
     1801 aaaaatgatt ttaggaagtt tatttagtgc aactgtattg ctaggtatgg taggaaccag
     1861 tgcgtatgca gatactacgt caccaggtga tccaagtgta ccaccgacag aaattattcc
     1921 accaacggaa gaaacacctg aagttcctga agaacctgtt ttaccaccaa cagatggagg
     1981 agaagaaacc ccagaaattc ccgaaatacc agaaactcct gaaaccccag agattccacc
     2041 aacggaagaa aatccagata caggtggagg cacagaaacg cctgaagtac cagaaaatcc
     2101 agatactggc ggaggggaag gaacacctga agttccaccc gttgaggaaa acccaggaaa
     2161 tggtggtaat acagagacac cagaaaagcc gtcgaatcca ggaggcaatg aaggaaatac
     2221 gactcctcca acgacaccac ctaccaataa tggggggacg aacactacgc ctccagtaca
     2281 ggacgtcgta gtaaaaccag atggaagtat tggcagccaa caaacaggtg gacaaaatgt
     2341 tcaaattgaa accaataatg cgtctgaatt aacccatatt ccaacaccaa gtacaccaat
     2401 tgaaacagct tcaggagaaa aaattgtctc cgtcgttgat ggtgtcgctt ataaagaatc
     2461 aggaaatggc ttgacgccca tttcagacaa ggtcgaacaa cttccaagcg gaaatattgc
     2521 ggtgaaaggg tctgatggaa aaatgaaagt attgcctaag actggcgaag aagtgtctct
     2581 cgttgcgaca gcacttggtg gattattggc ttcaggaagc ggttttgctt tttggcgcaa
     2641 aagaaaagca acacaaaaaa tgaactaagc gaatgaagga tcaaacatga agaaaaaact
     2701 tcttttatgg tgtgggttcg gttgtctacc cacactatta ctatgtatgt ttggttttct
     2761 gtttatgata cttttgatta gtagtggttc tgaagaggaa gaaatcgact gtagtccaac
     2821 cgaaattgtc gcaagtacta tctcattaac gaaccaaggc atggagacaa acgggcttca
     2881 aattgcaaag gaagtacaaa aacatattcc aaatacgaca ttagagggtc tagcgggtat
     2941 gttaggcaat tttcaacaag agtcgagtat gagtcccaca gcgattgaac gaccaaacga
     3001 tccattatct gggcatggga ttgcacaatg gacggcaggg cgaacgactg ctttgaaaaa
     3061 ttttgccaaa gagcgaaaca aagagtggtc agatttaggt gtacaagttg agtttttgat
     3121 tcatgaacta aacacaacag aaaaagcttc acaagttgca ttacaagcaa aaagcgtaga
     3181 ggaagcaaca gaacagtggc aagtgaaatt tgagcgtgca gggataccaa tgatggggaa
     3241 tcgtattact tttgcgaatc aatggtacgc aaaactagga aagaatgaca gtgatcccgt
     3301 cgcttcaatt acattagaaa atggggcaaa cgcaggaagt aaagacttga ccgattattg
     3361 tctagaaaca acaggaacaa gcagttcttc cgatagtaat gtattaaacg tagcaaaaag
     3421 tctgattggt tattttcatt attcacaacc gcaacgggca caatttggta gtgtcgaaaa
     3481 tccagataaa aatggctatg ccgattgttc gagctttgtg tggcttgttt taacgaaagc
     3541 agggtataaa acgcctgcga acgttgggtg gtttaccggc tcaatgacga gcgacgcaag
     3601 aggaagtaag caatacttac aagaaatacc tgagagcgag gcaaaagctg gcgatattat
     3661 cattgtgaac ttaggttctg gtgtgggaaa tgatggacac acagctgttt tagcggaaga
     3721 ttggcatgga ctttctacga aagtaatcga acaaggggga atgaatacta acggtgtagg
     3781 tgaagggcaa gtcaatttat cctttgggta tttgcttaat ggtggtgata tttgttttgc
     3841 tcgaccgata aagaaataag aggtgtcaaa tgaagaaacg atttatttat gtttgtgtag
     3901 gcggattgtt cttatttggt gtaggcagta cattttttct tattcatgaa aagctacagc
     3961 taacagcaaa ggaagagaaa ctgacggtgg agataaacaa gttccaagaa gagaagaaag
     4021 gacaggaaga ccttgtaacg tcactcaaag aacaacttca acaagtagaa agcgaggtca
     4081 cgacgttaaa aaaagcaaat gaagaattga aaaacaatga ttcaacagaa aaaggaaaca
     4141 ttcaaacctt tcaaaaaaca gtgacagaag cgttcgaggt tctgtttaat tatgagcctg
     4201 attcttttca agaacgaaaa aacaattgtg caccatatct ttctgacaaa ctgatgaagc
     4261 aatatttcaa agaaggcgtt tcctatggtg attcaaacgg ggtgtcttct gaattagaga
     4321 aatgttctgt ttatttaaaa agcacacaaa aagcgactat ccaagggctc gttgtggtgt
     4381 cgtatcgcaa taaaattggt gatagttcct ggaataatgc catgaatatt tttgacgtaa
     4441 cctttgatcc agcgtcaaag aaaatcacgt ttatgcaaaa tttgggaagt agctttaatc
     4501 aacagctttt agaagaataa gaaaaggaga aggttatgga gagacaagaa tcaataagag
     4561 aggtacaaga agtaccaaca gaacaagcaa aagaaaccca agaaggacgt tctttttctt
     4621 atggaaagaa tttttctttt ttacgcccgt cgattcaaat cagtgtgttc gttttgtggc
     4681 ggatacacaa taatcggtat tcggtaggca gtcaactatt ttatcaagaa atttgtcatg
     4741 ctttatcaaa aaaatacagt caaaatgcct acaaagaagc acttgccttt ttggaggggg
     4801 caagtgtggt tgtccacgaa gtagtgattg tggacaaaat accgaaatta ttgttagaac
     4861 ggtatggcat ttctcatgag taatgcaaaa tttaatccac aagcgttcca aactaagcca
     4921 ccgaaacaac agaaggaaaa gaaacaaaag aaacgttttt tgttccaaaa aaagaaaaca
     4981 gaaccgtcag atcgaatgat ccaattttcg caaaaaagtg ccatgagtgt attttcaggc
     5041 gctttttttt gtttgtttct gttaatgggt gtcgtgctcc tcctaaactt tggacgagtg
     5101 gatacattaa cgcgtttagc agtgaaaaaa caagtcaatc cagaaacatt gttaactgaa
     5161 gtcaaccaaa ccttgtcttc tacggaacaa ttgaagtacg agggacaaca ttttattgaa
     5221 aggttattta cttattcgcc agaaaaacga aacgaatggg aggcttccgt caatcattat
     5281 ttagcacaag gattggaagc gtctgatcta ggctttgtaa cgacggctac caatcgagta
     5341 gcgaaagatg ttcgctttat taaactagaa gaaatcgatt tgacacaagc aatgtatcgc
     5401 ctattttatg aggttacttt tacagaagga gaacattgga aaagcagtca actttctttt
     5461 ccagtcagtt atcaaaacaa tgttttaaaa gtacttgatg gcgttcaatt tttgaatctt
     5521 tctgagagta agaataccaa ttctgtgaac tatcaggaaa atcgatttta ttcttctggg
     5581 caagcggtca ctgaaagcga agaaatcaag ctggaagcat ttgtgaaacg gttttttgag
     5641 ttatatgtca aaaatgatga aaaattggca ttgatttcta acgaaaaagg gttagaaggc
     5701 ggcgaattga tcaatgtaca actggatcat gcagtaaaaa ataaagaagg aaactacttt
     5761 ttgcaaggga agtatcgttt ctattttgag aaaggaaacg agttccaatc aaacttttca
     5821 atggaagtaa aaacaagtgg cgatagttat tttgttacta cgttaaatga ataaaatgaa
     5881 cttagaggag aatggaaatg aaactttttg atcttaaatc gttatttgat gatgtatctg
     5941 gacaagtcat ttggctattg gcaatcgttg cgattgttct aatggttggt gcttatgcga
     6001 cccaaggctt tgcgcgggca gcgtttgcca ttattggcgt ctttattttg ataatgctcg
     6061 tcttattgtt atcgaatgcc gttgatattg gtgaatggct aaagaacatt atctttatta
     6121 aggatagtga agagaacgtg ggggtaacgc aaatgattgt gccctggata caattatcat
     6181 aagaaaaaga caaactgcaa aagcgatcag aagattagaa cttctggtcg cttctttttt
     6241 tgtaaaagga ggtagagtga acatggaata caactataca aaggagttta agcaccctca
     6301 caaaatttac gggattaaag gacaaaaaat tccctggatt tctaatggca ttcgcatgga
     6361 acaattagtc ataggtgggg tatttctctt cattcttttg atcctatttt tgattgcgta
     6421 tgtgaatcaa attagtttct tgcagacact gtttcgaaag tcctggttga ttatgatttt
     6481 tgggattggt gttctcgttt ggacattgtt ttcaatgaaa tgggacaata aacacttttt
     6541 cgagtattta attgggcgaa cgtcctatta tcagaaacgg aaacaacgct ttgaacatga
     6601 aatgtatgtg ccgtttttcc atgaaaaagt gacgtattcc ctacacaaat ggaaggagtg
     6661 agtcgagtaa atggcgtttt tgtttctttt ggtcgtcttt attatcggga tttggcagtt
     6721 caaaaagaaa aaaacgaagc ttcctcgatt agaatttccg atagacgcca ttatccgaaa
     6781 ttttgttttg accaaaaatg atgatgtgtg ggtaggctat caactttctt cccaagtatt
     6841 tcctttaaat gacattgatt ttttcaagaa ctatcttcaa gatggcgaag gcatacttgg
     6901 acatgatgaa tatgactatc attttatcaa tattccacgt caatttaacc tagatacgca
     6961 cattgaagaa accattcgag atttggtaaa gggagaatgc gcagatcttg ggaaagtgta
     7021 ctttgaacag gcaggaaaga ttttaaaaga cgaagtgcaa ttaaacgagt atcgaacgta
     7081 tctgtttgtg cgtttcacta cagaaatgaa agtgtctgat ccgatcgaat attttcaatt
     7141 gcttaagcat gtttttaaaa agtttatcta tcgtattacg ggacaacgag ttcctataag
     7201 tagtgtgtta ccaacgtatg aaacattaga aaaggagctc tatacggaat tgtccaatta
     7261 taagccgata gagcgcttat ctgcccaaga gattggacaa atttattatt attttttcca
     7321 tcgatcagaa gaaaaattgc ctgaaagaca gcttacgcca atggaaattt gcgagggcgt
     7381 catttcgaat gaagcaggat acattgcgat tgaacagttg aatcaaacgc attattcgac
     7441 gtttttaagt caaacggacg ctcctttagc catgtatggg agtagcttta ttcaaaatct
     7501 tcaggatagc ttatcttttc caattgatac gcatattcga cttcgatttg agcattcaaa
     7561 atctgatgaa cgaaaaatta ataaaatggc tggacggatt tatgaacaag aaaaagaata
     7621 tgagaccgtt gacggcattt tagattcaga cgatgtatta aattttggta cagaacggtt
     7681 aatggatgta aaagataagc tgaagcgcaa agaaaaacga ttgtgttatt ctaccgtcac
     7741 gctggttgta tctgcttctt caaaagaaga attggaagaa cgagtcaaag atattgaatt
     7801 tgcgattgag tctacagatt tcaaatttta tcgatcggta gcggatcagc taacgttgtt
     7861 taatcaatgc ttaattggaa gtacgcaacg ttttcgtagt tacgagcagg tcgctacgac
     7921 aggttttgtg gcagattatg gcttggactt aaagaaagaa gtaggaaata agaaaggatt
     7981 tcctttagga cggattgtta ttgcaaaaaa gtttaaatct gcgaaagaag cgttagcgct
     8041 atctagtaaa ctagtatggt attatcccaa tttaacaaag aaaagtattg aaggcgcgga
     8101 acataccaat ggaaatacgt tgattattgg tccaccaggt aaaggaaaat cggtccttgt
     8161 aaaatatatt tttttatggc tgatgttttt aggtcaaaaa attctttata ttgatccgaa
     8221 aaatgaaacc caaaagttct ttttaaaggc acttgaaaaa ttcggacaca taccagagtt
     8281 tgtggctatg tatgaacgaa ttaattttat ttcgttatct tcagatgaaa gctgtcgagg
     8341 gatgcttgat cctctattgt ttttaccaaa agaagaggca attcaaaccg ctaaaaatgt
     8401 gttaggaatg ttaggagaag tagataccaa tcgagaaaca gcgagccaca agaaaacggt
     8461 gatccaagac gccatcgatg tagtttacac catgccaggt aagcattcgt taagtcaagt
     8521 tatcgaagaa attcgaaaaa gtgacaaaga actagctaaa ttgattttag ggcatagcgt
     8581 tggtttagga aagattctca ttgggaatga gcacagtacg ccgatacgtt ttgattcaca
     8641 aatcaacgtg ttagggacac aaggaatacg cattccgaca caaaaagaga ttgattcggg
     8701 tcgattaaat agcgaacaac ttgcaggaat ggcaattatg gaagtcatta tgaagtttac
     8761 gtatatcttt tctacaaaca aagaagaaga cgctgcgatt atttatgacg aggcaaaagg
     8821 gtatgaagat accgcacaag gaagctattt gattgatgat agcttgcgaa aaggacgtgc
     8881 gaataatacg gatatttacc ttgttgtgca agcgtttaaa gattacgatc aagaagataa
     8941 aaaagagtta atttcttaca aatttgcgtt tcgtccgaaa caaaaagaag cacgagagaa
     9001 actattgtcc ttctttgata tggaagtgaa ttcagccaat ttagaaatga tggaacaatt
     9061 acaaacagga acctgtttat tccaggatca tttgggaaga aatcaaccga ttgccattga
     9121 tgtgttgttt gatagttggt tattggctgt ttcttctact gaaaatcaag atgaaaaaat
     9181 tcaagcaatg ttagaaaatg aaaaacaacg ataaaacaaa aaagaggtgg tttttttgcg
     9241 aaaaaaatct ctgaaatggt ggacgctcct tttattaggg ggtctttttt tctgtctaca
     9301 aggggctact gtttctgccg atcaaatgaa tgatggtgcg ggtgtcactg aaaccgatcg
     9361 ttctgaagga gagaatgcca acgataaagt agaagtaaaa gaagtaacga ctgatgtgga
     9421 tcaattgtat aaaaattaca aaaagaattc gtttgaattg atgaccaatg actatgagtc
     9481 aatgaacctt aaaaaaaagc tatcgtacaa tgttgcagga gtgatgaaaa attttgtttg
     9541 gagtggcgta aaaatgttag gagaattcaa tgcttcaatg gttgaatttc tctttgaatt
     9601 agatattatt tccgccattc gagaacccat tcaaaactta acacaaaaaa ttgcggacaa
     9661 tatgttaagt attgcaggaa ctattggaat cagtttttcg gcaattatga tggcaatcaa
     9721 atatgctgga gaggctcgaa tgaaacaagc actccgtgtc tttcttatga cgatccttat
     9781 ttttactggg ttaagcgtat taaaagattc tgggacaaat aagaatcttt ttgatgttct
     9841 tttttcttta gattctacag tggaaacggc gttcgtgaag gtcaatccag tgttaacacc
     9901 tgaagaagaa acaaattatg aaacaaaaga aacagaagag gtaacagcgg ataaaaaagt
     9961 ggaaacggca ggcaaattga tggcggctcg ggtcttttat agtaatgttt atgaaccata
    10021 tctgttgtta aattatggca caaccaatgt agacacaatt cgtgagaaga aagtcaaata
    10081 tgctggaaaa gaatatgatc gaatcaatct tttattggac aatgatttag gtacaaaaat
    10141 aggggagaaa attcatgaca aagtcacaga gtatgaagca gatacactga aaaacaagaa
    10201 catacaatat tacaatagtt ggaaaaatac agcatttgcc ttgttttatt ttgtcgtgaa
    10261 tctgattcaa accgttgttt attttgtctt aagtatgttt cgattgatta ttggaactat
    10321 gcaactattg ttagtaccga tgttgccgtt cttacttttt ttaggattgt ttcttgtcac
    10381 aatgaatgtg tttggtaact atgccaaagt ttttggtatt ttgacgtttt taaaaggaat
    10441 gacaggtttt gcatgtatcc ttttttcttc ttttcttagt tacgggttcc aattaagtag
    10501 tacacaagac gatccatggg aaaaaattct cttgatcatt gtttatttgt ttgcgccgtt
    10561 gggaatttat ttgttccgag gctttttagg cagtctattt actggaagaa tgggtgttcg
    10621 aggagcgtta gcttttgcgg gaaatccatt tggggcagaa gcaaaattga gaagggaagc
    10681 caaacaacgg ttgaaagaac gcaaagaagc gagaaaagca cagaaacaaa aagaaaaaga
    10741 aaacgcacgg aagagacaag aacgtcaacg aaaacgtggg ggtggctcag acgttcgtat
    10801 gccacaaacg gcgaaacaag ccacacgaag tgcttttcgg cgtgaatccg cacaacaacc
    10861 tcgtcataaa gcaccaaatc gtatggaacg tatggcacaa aaagcaagag acttgcatga
    10921 tcaagcgaaa caagcagaag agcaaagtca agaacgtatg caacgaacag caatggagaa
    10981 acaggcaaga aaaacagccg atttattaca acgagaattg caaggcaatc ctagtctaag
    11041 agaaagaagt agaaaaggtg cgtctcgtag tccgtatcgc aatcagggtt ctgcaatggt
    11101 ggtgtcatta gcgaatccaa agcaaaaaca caagtcttat ggccagaagc atacgcctag
    11161 aacagaattg aacgatcgaa aacgtcgtca gggacaaaca agtcgtcgtc ggaaggaggc
    11221 acttactccc gtaacaaaaa caagttcgat gaaaacccgg acagttgcag gacaaaaaca
    11281 tgcggtacaa aaaccactaa gaaaagggaa tgtaccacga gcaacaggtt ctaccacacc
    11341 gaaatctgtt cgacgtccag agggggttcg ggaacatatg tcagcagtca atcgagtaac
    11401 agcacaaacc aaagcgcctt caaccgtatc taaagcatac tcaaccatga aacaaacaaa
    11461 atcaccaaaa caagcaccga agacacggag tgagtttcga gcaactcaag caaaaatgaa
    11521 acgaaaacga gaaaaacaac cgatttatcg aagagaaatg aaacaacgaa aacgatagga
    11581 ggaaatcgat agtatgatga atgcaccaaa acgaccgaca cattttgagc aaatgttttc
    11641 tgtcgatcgc aaaagaaaac aagaaaaaga aacgccaaaa agtcggtttc aacaacttta
    11701 ttttatcgtc actttattag gtgtttgtct atatccattt tttattgtag gcagtattcc
    11761 tttagcgatt gtctttatga tagataaaaa agagacacgt gcacatattt atgactttga
    11821 gtatgagagc ttcttaaaac gaagaagctc tctttttttt atttttgcgt taggttttac
    11881 gctattaaat ctattttgtt ttatttttgt tcttcctaga gggtatttgt cgtgttactt
    11941 attatttcca ttaaacttgt tttctcatgc gttgcagttt aatggacaaa cgatttttgc
    12001 cctcctttta ggtgggacag gtgcaggaag ttgtttgatt gcgtatgcgt cttttattga
    12061 aaaaagaaaa gttgtttcaa agaaagaacg gcaaacagct ttgctaaata gtaaagaata
    12121 caaggaacgt cggaagaata aatttcagga gtcccaaaaa tatacagcag agtatcagca
    12181 agcgtaccag gtagcaattg tcataaaaga cgttgctttg cggaataaac aactggcttc
    12241 attagccaaa gtgattctat taggaacaga tgagtatggg ctcccttatg tgatgaactt
    12301 tagtgaattg aaccaacatg tgattattcc agcgactaca ggttcaggaa aaacgacggt
    12361 gatccaatta ctagcagaac atgcgtgtaa gtttcgtatc cccgttatct tagtcgatgg
    12421 gaaaggagca ggagagactt tacaagcgtt aaaagaagtg gcagaggcat acaatcgccc
    12481 gattgaagtc tttgtggacg gtggcgattt gcgttataat cctgtaaaaa atggcaatga
    12541 tattgccatt cgagataaat taaccaatct agcagaaaca gaaagcgtgt attattcgac
    12601 agcagccaaa gcgttattac aggtaacgat tcagctttta gacgagttcc aaaagagcca
    12661 taaaattgaa agaaatctag tttggttaca acattattta ttgccacgaa acgtcttaca
    12721 tttgtttgcg gatcgtattc agttgaaaaa tccagattta tttaaagaaa aagtggtcgt
    12781 agaaaagaag aaaaccacga agaaaaaaac gaaaaagaat gaagcgatgg acaaaacaga
    12841 acgtgatttt gccgatgtgg aacagttaag tgaagagcga atcgttgaac atgatcgtaa
    12901 aaccgttgac ttagaaacgt attatctatt attaaaagaa aacatttctc tcatgacaga
    12961 aatcgagcaa acgttgttta aacggttgtt tgttcgttac gaacataaga agaacccgtt
    13021 ttatttgtat gcgacttctg agaatttaca gaccaatatc aatatgttat tggatagtga
    13081 gcttggtgaa ctctttgata cttctggagg aaaaagagaa ttagatattc aagaagttgt
    13141 taaagaagga aagattgttt atatctcgtt aaacggattg atttatagtg agtttattcg
    13201 gacattggct caaatgcttg tgggagatat taatttcttt gcgtcagaag tgtataaaca
    13261 agcaggagaa acagccaatc gctcgaaagt cctagtaata tttgacgaac cagcttctta
    13321 tctaaatgat aaccttatag acttagtcaa taagggacga ggcgctgggg tgcatgcggc
    13381 gtatgcccct caaacgcttg cggacattga tagaattgat cctgtccttc gaaaacagtt
    13441 gattgggaat gtgaatacgt tctttattgg gaagacaaat gacccgacag aaattgatta
    13501 ttgggcagat ttaatgggga cagaagaaga cattgaatta acggaaatga cgacacaaga
    13561 agcaggttat tctgatatgg gaaaggttga ttggacagga gaaagaggca cgaaacgaga
    13621 cgtagaccgc tttaaattca atccgaatcg cattcgagca ttgagaacag gcgaatttgt
    13681 ggtgtatcga acagctgaag acgtccagga accaccgcga gtcgtctata ttcggaaacc
    13741 aaatttggag aaacaatatg cgaaataaga aagttattgt taggatactt attgttagtt
    13801 tcctcttatt tggttgtttt aaactgataa aaactaacga agaatcaaat ggaaaactaa
    13861 tcaatagtaa tttagaagaa gtactagaac ttcaaaaaac gaatcaatca ttttttgtgt
    13921 atctagcaag aaatgattgt aaaaaatgcc aagaagttga atctttcttg gaaagatacg
    13981 acaaaaaatt ctctaggaat gtatatcgaa ttgaaacaag aaaagaacca agacaagaag
    14041 aacttcaaac ttttttacaa cgttaccatg ttaaaagtgt cccaagtttt ctcgtgattc
    14101 atgatagaaa cgtcaaaaaa attgagagaa aaacattctt ttttgaaaag acattgaatc
    14161 aatttcttcc taatttttag tgaaaattga aagagaatac gttgaaaaaa acaaaaaatt
    14221 gttatgagtt caaatttttt tcgatcgtat ttgtttttct tttgaaacaa attcactcat
    14281 tttttgagca aaaatgtttc aaaatttgaa caaaaaccaa ttgaaaattg aagcaaattt
    14341 cactcgaatt gaatcgattt tgagctaaaa tcaatagaaa aaataaacgt attttgtttg
    14401 aaatccgttt atttttttta cgtttcagta ttagtaagga ttatttgagg gttatttaga
    14461 gattatttaa aggttatatg taataagtgg ggggagggct gtcggcgctt ggtatgaaag
    14521 gcattctccg acttccaggt ttttgaccat tttctctcaa cgtggaaaaa ccgccactta
    14581 tgagagaaaa tggtcaaaaa cctaagcggg aagtctccga ataacatacc aagcgccgac
    14641 agccaacaga taaagaatac actactaaaa atagtagtag cataaaattt aaaagggaaa
    14701 aaatcaaccc ctaccccagt cttggggtct caaaactaac tcccccctaa ccccctcttt
    14761 cagcctcttt tgaaagaggg ggagaatcaa ccccacggcc aacgccccag tgttcttggg
    14821 gctgtccaga acagaaactt gcgagagagt gggggtaaaa caagtgtaga ggaaacttga
    14881 agacttgttc gaacagaaac ttgcgagaga gccggagtaa aacaagtgta gaggaaactt
    14941 gaagacttgt tcgaacagaa acttgcgaga gagttggagt aaaacaagtg tagaggaaac
    15001 ttgaagactt gttcgaacac aaacttacaa gagaggggag taaagcagtt gcagaggaat
    15061 caggaagatt tgttcgaaca gaaacttgca agaaagcatt attaaattga aggtgatgaa
    15121 atatgaagta tttattgcat ggctatttta ttgattattc tactcgcaat agtagtactt
    15181 tagcgattct atgtttgctt catgaattga gagtggttac tagaaaacag ctatacaaac
    15241 tattgaattt tgacagatca attagtaaac aaggagtaac gaatgcttta gtgagattat
    15301 atgaaaaaca gttaatcgat cgaaaaaaat atggaacaga aatgtattat tatttgacga
    15361 aagagggaca tcaaagcata ggtgggtatt atacgttgcc gaaagttcct gaatataatt
    15421 tgaatcacca tttacaaatt aacaattatt tgattaaagg aatggcattg gcaaaagacc
    15481 acccacattt aaaacggatt atttcagaaa gacgtaccgt ctatgaaacg aaggatttaa
    15541 atcctaagac aaaaggaaga aaattttttg tgccagattt tatttttcgt tttcgagaca
    15601 aagaaaatca tgagatcaat tggtcctttg aaattgagct aacgttgaaa acacgtcgtc
    15661 gctaccgtga agcgattttt ccgaaatata ttcgtgagtt gcgaacacgt cctgaatcac
    15721 atttaattta tgtgacacct agtggcgcaa ttcaagaaga acttcatgcg tttaagcgta
    15781 tttttttcaa gcgcgaaact gaaaaagatg cgagtcaaca agaattgttt gagcggttgc
    15841 atattttttc agctttagag tttgaagagg gattaaaagc attattagag aatgatccag
    15901 cagtcaattg gcggacagta gaacgagaaa aaatttatcc gaatggcgct gttcaaagtc
    15961 gtttgaattt atttgaagaa tcaactgaaa atgaggaaaa accaaaagtg aagtttacgc
    16021 ctgaagtatt gtaaaagagg gtgttcgctt cgctcttttt ttgtgccagt ttgctggcac
    16081 cgttcagacg caagcgtctg aaccggctcg tttttaggaa ggactaactt gtttgattta
    16141 tccgttgtca aagaagtgga cgttcgagag tccctaatcg tatagcagtc attcggcgag
    16201 tataacctaa aacataacct gccaattgcc aagggtaaaa tgcacgctgc gcgcccttgg
    16261 caatcgtcag aacatgtttt cgagccggtt atgccgaacg accgctatac gaaaagggcc
    16321 tctaaacaga agtccacttt gatccttgac aactgaaacc caaaatcaaa caagcaggcc
    16381 ttcgtctaaa aacgagccga atgaagacct tgggggttgc acccccaaac ccccgtgccc
    16441 ccgacacact accgaaaagg tgggggtggg tggcgaaaaa atcaaataaa acattctatt
    16501 caagaaagga agaaacaacc atgacaacta aaaacgagca actgaaagaa caggcaagaa
    16561 aagagttgca agaaagaggt ttgattattg aggggagctt tgaaggagat tttgaaacct
    16621 atattggttg ttatgcaaga ccgataaaca aacctacagc cttagaccca atgaacgaac
    16681 aagaagtaaa agaacaagaa aaacatgcag tgaatggttt tccgcaagat ttcactgaat
    16741 ggtatgagtg ggaaattaac aacgggaagc ttgaaaactt cttataaaag tcagatgacg
    16801 tgcaaagaga atgattctct ttgtacggta aaaatataaa gaaagaagga aaaagaaaat
    16861 ggcaaaaaac acaccttatc aagtaggaga cattttttat agtagttggg gctacgaaca
    16921 aacgaacgtg actttttggc aaattgtgaa gttaacagaa aaaacagcat ggtttcagcc
    16981 tattcaaaag aaaacggtcg agcattattc gtccatgagt ggaaaagttg ttccaatgaa
    17041 agacaagttt atagagtatc ctttagcaaa aactaaagac agtattgtaa aaaaacgaat
    17101 tgatccagaa acctatcctg atatgttatg tggttatcgt agttgggaca cgtttcatag
    17161 atataaaaat caagctgttt atgaaagtag ttattattaa ggggatagtt tcccctttca
    17221 aaaataataa atagaagaat ggagagaaaa aatcatgatg aattcagtga atttagtagg
    17281 acggttaaca aaagaagtgg atttaaaata cacaccaaaa ggcaatgcga caggcacatt
    17341 tattttagct gttaatcgaa attatacgaa tgcaaacggc gagcgtgaag cagattatat
    17401 ccgctgtgtc atttggcgga aagctgcgga aactttagca aaatatactg gaaaaggaag
    17461 tttgattggc attaatggac gtcttcaaac gaggagctat cagaatcagc aaggacagac
    17521 catgtatgtt acggaagttc tagtgacaga tttctatttg ttagagagta aagaaatcaa
    17581 tgagcaacgg tcaaaatcaa tggcgaataa tcaaaataat tcttcaattc caggaaatga
    17641 gattcaaatt tcagatagtt atttgccctt ttaagtgaaa aataattttg ttctatacct
    17701 gatttcttca ggtatagaac aaaaaataaa tagagatgag gcgatttttt atgaaaatca
    17761 ttgacagtta cgatcaacga accatgcaat atttgggctc gtttgattat acagatgaaa
    17821 atattattaa ttatgttgca agtttacctc cttatcaaag cgttcgcttg gtagattttg
    17881 caagtgatga attggtatta acaacgatgg gcaacttttt agattatgtc ggagacaaaa
    17941 aatggttgga aaaaattttg tctaaattga ttgcgaaaca aacaggagaa gaacccataa
    18001 atgcgattca attgttcaat cgatatgaac aagaaaacaa tgattaaaga ggagagaaaa
    18061 aatgaaaaga ttaaaacgaa gtatttttat tgtgctagca ttgattgatt ttgttgggac
    18121 aggttgtgaa gcaattgacg ttcaaaaagc tcccttatcg gaaataaaac aagcaataac
    18181 aaaacaagat gaagaacaac cgctaaatac acaagtagaa atagtgaatc aaaataaacc
    18241 gagtttttct gaagaagatt tttcgttaga aaatggaacc aggcaatttt tttcagattt
    18301 ggatcgttta aatcgggtag gtgtagcaag tgcacttttg cataaaagca ggatgccaac
    18361 agaagaacga gaccgattat acattaagcc aactggctgg aaacaaaaga aaatgaagaa
    18421 tggcgattat ctttataatc gttgtcattt gattggctat caacgtgcga caagtttccc
    18481 cataagtatg taatacgaaa cgggaggatt aatcttaaga ttaatcacaa ctaacctatt
    18541 tgacagttgg aatgaaagct atcatgtctg ctgaatcaac tggtctgata ctcctacatg
    18601 caataaagtc tactaggaag ttattgtatg aagctaggta aaggcggcag aacaatttct
    18661 aagtgtggaa tcatatgaag ttggatatgc cgggacgcta taaattactc agggtgagac
    18721 tgtttttaat taaactgacg aacttgcgaa tgtacgggtc tataaccgac ttatattcga
    18781 aagatataag attgcgaatg caaagaacat tatcgaaaag taagaatgtt ggtcatgaaa
    18841 ttcgagatgg aagactagcg gtgtatctgt caagagttgg taaaacatgt ttgattgatt
    18901 cagatgagag tgggaatgcg acaaaacgtt ttacagaaga aatagagcaa ctagcagaac
    18961 tgaatatacg atatttttaa atactttagc ggagaataaa aaaatcctct acagaaaact
    19021 acggagggca acatgtgcta ttgtttcaac aatacatttt taatttttac cataacaaaa
    19081 aagtacttga aaaaaaacag aaatatgtta aattaaaaat gaaaaaatgg aagcggttac
    19141 ttattctttt gaatctaaga taaaaattca aaagaataac ataacccaaa tatgattata
    19201 cttgagattc taaatattta tcgaaaagga atttgtttta aggaaaaatg attctatgga
    19261 cggttattga tttatattta ccaaatggct aataaaatag tatttcttat attgtatagt
    19321 ttttgttttt atgtgaaaac atgagcaata gaattgtact agtaggataa ggatggatgg
    19381 aaaatgaaaa aaactatttt tgttttagcg atttttatat gttttgggct acttttggta
    19441 ggctacttgt tgtttaattc taatgaaatg agaactattt ctacgaatga cttgttaaaa
    19501 aaaagtgaat taaaggataa aaaaaaagaa tctcttgcct ttataatgtt tactcaagaa
    19561 gagtgtga
//
LOCUS       DACCIQ010000048        19541 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCIQ010000048 DACCIQ010000000
VERSION     DACCIQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.048"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(567..1592)
                     /locus_tag="IVM62_001708"
     CDS             complement(567..1592)
                     /locus_tag="IVM62_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6845673.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1867..3552
                     /locus_tag="IVM62_001709"
     CDS             1867..3552
                     /locus_tag="IVM62_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP6845674.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3566..4849
                     /locus_tag="IVM62_001710"
     CDS             3566..4849
                     /locus_tag="IVM62_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6845675.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4919..5788
                     /locus_tag="IVM62_001711"
     CDS             4919..5788
                     /locus_tag="IVM62_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6845676.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5785..6621
                     /locus_tag="IVM62_001712"
     CDS             5785..6621
                     /locus_tag="IVM62_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6845677.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6647..7714
                     /locus_tag="IVM62_001713"
     CDS             6647..7714
                     /locus_tag="IVM62_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP6845678.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7711..8538
                     /locus_tag="IVM62_001714"
     CDS             7711..8538
                     /locus_tag="IVM62_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6845679.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8583..9608
                     /locus_tag="IVM62_001715"
     CDS             8583..9608
                     /locus_tag="IVM62_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6845680.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9605..11788
                     /locus_tag="IVM62_001716"
     CDS             9605..11788
                     /locus_tag="IVM62_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6845681.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11788..12918
                     /locus_tag="IVM62_001717"
     CDS             11788..12918
                     /locus_tag="IVM62_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6845682.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12915..13592
                     /gene="pgmB"
                     /locus_tag="IVM62_001718"
     CDS             12915..13592
                     /gene="pgmB"
                     /locus_tag="IVM62_001718"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6845683.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13676..14203
                     /gene="msrA"
                     /locus_tag="IVM62_001719"
     CDS             13676..14203
                     /gene="msrA"
                     /locus_tag="IVM62_001719"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6845684.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14404..15090)
                     /locus_tag="IVM62_001720"
     CDS             complement(14404..15090)
                     /locus_tag="IVM62_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP6845685.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15170..16708
                     /locus_tag="IVM62_001721"
     CDS             15170..16708
                     /locus_tag="IVM62_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP6845686.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16828..17295
                     /locus_tag="IVM62_001722"
     CDS             16828..17295
                     /locus_tag="IVM62_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6845687.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17814..18257
                     /gene="rplM"
                     /locus_tag="IVM62_001723"
     CDS             17814..18257
                     /gene="rplM"
                     /locus_tag="IVM62_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP6845688.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18273..18665
                     /gene="rpsI"
                     /locus_tag="IVM62_001724"
     CDS             18273..18665
                     /gene="rpsI"
                     /locus_tag="IVM62_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6845689.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18753..>19541)
                     /locus_tag="IVM62_001725"
     CDS             complement(18753..>19541)
                     /locus_tag="IVM62_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6845690.1"
                     /translation="IIKGYVRSMFDIAEILNYIEFNRTTKIIQSITAPKKNALEEKRI
                     QEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYID
                     FEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQT
                     LDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGG
                     ADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6329 a         3532 c         4148 g         5532 t
ORIGIN      
        1 tattgtcaca acctcttttt tggtttgtct aaaataattt gtctaactag aagaaaagtg
       61 ggaaaggatc ttaacaacat cggttcgtat ccaattaagg tatcggaacg atcatcttcc
      121 aagagaagtc aatcaacaaa agtaaagata gatcattttt tgaatgcaaa ataaattgat
      181 gggaagatag agcggttatt tttaatattt tgctagctat tattgaataa ttcctcgaat
      241 aaaacaaaac cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca
      301 aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata
      361 cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat
      421 tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg
      481 ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag
      541 tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga
      601 gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg
      661 aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac
      721 tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca
      781 ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg
      841 gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg
      901 ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga
      961 tcaaataagc attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga
     1021 gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa
     1081 ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt
     1141 ccgattacca caataggaat attgtattga tccaattcaa aaagaagtgt ttcatccatc
     1201 gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt
     1261 tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca
     1321 gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga
     1381 gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca
     1441 attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta
     1501 tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga gacattagct
     1561 tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg
     1621 ttttagaata attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta
     1681 ggaatagctt gcatgatcat gagagaccat atttttttaa gaagctatta attattgata
     1741 cataagcttt cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga
     1801 cagtaagcgt tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg
     1861 aatccgatgt atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa
     1921 accgcctcta tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc
     1981 aaactgcatt gggacgaaga agatattttc ttgattactt atggggatca attttttgaa
     2041 gagcagcaac caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca
     2101 tttagagttg tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt
     2161 gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga
     2221 agcgaaacac ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg
     2281 tttcaggaat atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg
     2341 gatacagata caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt
     2401 gacaaaaacg ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg
     2461 aattttaaaa atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa
     2521 aaaggcgctt catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact
     2581 tcttgtatac acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat
     2641 gaagcagctc caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc
     2701 agttattttg ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct
     2761 ttgacccttc aagcgattcg ttcgggtaat acaagtaagt tgaaagaatg gttaaaaacc
     2821 atcgatttcg catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc
     2881 ggattgaatc cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag
     2941 ctgaagaaag agggtacccg agtaaactac aagaaaaacc cagatggaac gaccagtcca
     3001 tatgagataa acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg
     3061 agaagcaaac gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca
     3121 atttatattc aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga
     3181 gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag
     3241 acagatccat taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa
     3301 aaagaaaaag ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt
     3361 atcagtttta ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat
     3421 caatcagcag gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt
     3481 atccattgca gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa
     3541 ataataaaat aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc
     3601 tactggattt cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac
     3661 aacgattcaa ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact
     3721 ggttgctgat tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga
     3781 atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat
     3841 ggaagtcagt caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc
     3901 agtccaaaac gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct
     3961 tgttcgttca gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg
     4021 tatttggtat gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga
     4081 tgatatttta aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc
     4141 tatgcctact gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa
     4201 tggagcaaat gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga
     4261 agcattgtcg ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac
     4321 agaaatcaaa gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat
     4381 tttgccttct gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca
     4441 aaaagatcaa gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga
     4501 gaaacaaaaa gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga
     4561 aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact
     4621 ggatggttac aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc
     4681 tagtgctttt gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat
     4741 gggagatatc acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa
     4801 cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct
     4861 gccaatggtg ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat
     4921 gaatcgtcca aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat
     4981 gcttttatta gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt
     5041 cgatatgccg ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga
     5101 aattttaagt gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt
     5161 agttgtagca ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt
     5221 cagatttagg aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc
     5281 tttgattttt gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt
     5341 agtagatagg ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag
     5401 cttctttatg gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt
     5461 tttggctatt ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc
     5521 aaatgcttgg cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac
     5581 tgtgattgtt ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac
     5641 gaataaagtc aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct
     5701 tggaaaagca gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct
     5761 ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac
     5821 gcgacgattt tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca
     5881 tcattcaagg attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg
     5941 acttttgaac attatcagga tattttcaat gctgagattt ttccattcat catgtatttc
     6001 aaaaatagtt tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc
     6061 ggggcatacg ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt
     6121 tacaccgttt atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc
     6181 ggtctaggtt tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt
     6241 cctactgcga tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa
     6301 gcagcaatga tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg
     6361 gctatttctg gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat
     6421 ttatttgctt cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac
     6481 acattattca gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc
     6541 gctttaccag tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc
     6601 gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt
     6661 tgcattagaa ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa
     6721 cgaagtacgt atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt
     6781 tagaggatta agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc
     6841 aagagaagaa ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat
     6901 tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt
     6961 ctgcacttat ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact
     7021 acgtaaactt gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt
     7081 cgctttaggt ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg
     7141 tttaggcgct atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat
     7201 cgttgggatc gatcccatag cgcagcgtag agaattagca gaaaaatacg gtgccgatta
     7261 cacgctagat cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg
     7321 tggagtagat tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaag
     7381 agggatcggc tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg
     7441 gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc
     7501 tagtgaacca aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg
     7561 ggaattattg atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt
     7621 tgcagaaagc gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa
     7681 aatgggcgtt caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt
     7741 ttcccaagag acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac
     7801 gaaatcgacg gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa
     7861 acagggatcc cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc
     7921 gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag
     7981 gttggaggca aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg
     8041 ccgatggttt caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt
     8101 tatttagata aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga
     8161 taccaagacc atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa
     8221 ttgaaacatg tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt
     8281 tcaaaagcat tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat
     8341 cgctaccaac caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa
     8401 aatgagtatg aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa tgaagtgaaa
     8461 atttatcagg aatcattgca atacttgaaa aactgtctgg aaacagcgcg tcatttaaat
     8521 gatttagaga aagaatagag aaagaataac aataagtaaa taagcaaagg agggagaaat
     8581 aaatgaagaa agtagcaatc ataggcgctg gacaagtagc agaaaaagta catgctgctt
     8641 attatcaaaa aagatcagat gtcttagaat tggccgctgt ggttgatcca gtcagccagc
     8701 gagcagaaga cttttgccga cgtacaggat tttctcatgc gtatacatca gttacacaaa
     8761 tgctgaatga gatttctccc gatatcgtga gtgtctgtac acccaatcgc tttcacttcg
     8821 aacatgtatt ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa ccaccagcta
     8881 tgacagctga tcaagcaaga aagatggcag agacagccga taaaaacggg cagattttgg
     8941 cttatggatt ccagcatcgt ttttctgacg aagcaacaat gatcaagaat catcttgatg
     9001 ttctcgggga tatttattac gtagaagcga atgcgcttcg tcggtctggt gttccaggct
     9061 ggggaacgtt tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat tatggcattc
     9121 acatgcttga tacagcgatg tatattactg gatttcctaa agtaaaaagc gttcgggcgt
     9181 acagcttcca aaaaattggt acgaaaaaaa attcagggaa attcgggaaa tgggatccaa
     9241 gcaaattcac agtagaagat gcaatttttg gcacactgga attagaaaac ggcaagatca
     9301 tccaactgaa tacttcgttt gctttgaata tcagagaaga caaactgaat gtggcttttt
     9361 gtggcgatca ggcaggtgct aatctgtatc caggcgaact attcgaagat gtagatggaa
     9421 agctggtttc cttacaaaag accacgccag ttaatgaccc acatgaacta agtatggctc
     9481 attttgtgaa tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc tggcaaggat
     9541 atgtcatcca gaatctggtg gaaaatttat atcgttcggc agaaacagga gaagttatta
     9601 ccttatgaaa gtagaagaaa aacagtttga tttagagtat atcaataagt atgcgagtca
     9661 gatgaccatt ggaaatggat tttttggtat cagaggaagc caggaggaag actatcggaa
     9721 tcaagtaagg ggaatgtttg cagctggtgt atataaccga cctttaggag cagaatcagc
     9781 cgagttagtg aatctcccag atgtcatcag atgggaaata aaaatcgacg gtgaacattt
     9841 ttctcttgag acgatgaaag ttcttgcata cgagcgggca ctagatttag aaatgggaga
     9901 gcttaaacgc tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag tcattacttg
     9961 tcgtgttgct gatcaaaata atctccatca ggcagccctg aaagtggtca tcaagccgtt
    10021 gaccgaagac tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa ccaattttgg
    10081 tacacagcag ttggttgaaa aagaactgcg agtatttggt gacgatttga tcagtgcatc
    10141 ctaccagaca acagaaagta aaattgatat tgggattgca gctaaattca ataaagcagg
    10201 tgtattccaa gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga tcagaaaaaa
    10261 tacgtccttt acgctggaga agatcatcgt gctctctacc tctcttcagc aaacagaccc
    10321 gtttaagaaa agtattgccc aagcccagaa aagcgttagt tatacagaaa ttttagagca
    10381 gtcgatgtct tattggacag atttttggca tcagcatcgg ataacgatcc aaggcgattc
    10441 ttctttcgat cagttagcga tggacttcgc tgcttaccat ttacagatta tgactccccg
    10501 caatgatcca agtgttagtg taggggcgaa gggactaact ggcgaaggtt ataaagggca
    10561 tgttttctgg gatacggaaa tttttatcct gcctttcttt ttatatcagt ttcctgaagt
    10621 agcaaagaat ttgctgctgt accgttatga tcgattagaa ggcgcacggc aaaaagcaag
    10681 agattatcac tatcaaggtg cacttttccc atgggaaagc gcacacactg gtcaggaaga
    10741 gacacccaaa tttgctgcat tgaatatcaa aaccggaaca agacaagaag ttgcttcagc
    10801 tgccgcagaa catcatatca gtgcagacct tgcttatgcc gttcaagagt atgtccaagc
    10861 aacagaggat caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg aaacagcagc
    10921 tttttggctg agcagagctg taaaagtcga tgatcattta gagatacaca gagtcattgg
    10981 tccagatgaa tacacagaat atatcgacaa taatgcatat acgaactatc tggcgcatta
    11041 taatgtggcc ttagctgtta aatatcaagc tggcgatccg ctttttttga aagaatgtca
    11101 aaatttttta gataagttat atttgccagt gcctaacaaa gaacagctga ttccgcaaga
    11161 tgacacgtat ctcagtaagc cagttattga tttgaccact tacaaagaac gtcctggcag
    11221 ccagtctatt ttacttgatt atgcccgtag cgaagtaaat gaactccaag tcagcaagca
    11281 agcagatctt gtcatgctgt tatacttatt acctgagtat ttttctcaac caatcgtttc
    11341 taagaatttg cattactatg aaaacaaaac aatccacgac tcatcgttga gtaaagcgat
    11401 tcacgccata gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt tattccaaga
    11461 agcgtgcctg attgatttag gaccagatcc gcattcatca gatgacggaa ttcatgctgc
    11521 ttcattagga gcactatggc tagcagttat atttggtttt tcaggtatta cagtaacgaa
    11581 tggaactctt tctatttctc caagacttcc ttcagcatgg gaaaaaattt cttttccttt
    11641 tatttggaaa aatcacaggt tgctatttgt tttgacaaaa aaacaaatag taataaaaaa
    11701 agagacgaaa gaaacattac ctatttttgt caatggagac caataccagc tggaaaaaga
    11761 actgatcttg gaacggaggg gaaaataatg cgtattctga ttgcgccaga ttctttcaaa
    11821 gaaagtctga cagccttaga ggcagcacaa gccattcaat caggttttca agaagttttt
    11881 gttcaggctg aatatgatct gctacctata ggcgatggag gagaaggaac ggtttcatca
    11941 ttagcagaca gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc atttggcaaa
    12001 aacaaagaaa tccagtacgc ttcagacggt caaactgcga tttttgaaat ggcagaaatc
    12061 gtcggattga aagatgtccc actaaaccaa caacagccac ttaggttatc cacgcgagga
    12121 gtaggagaac tgatacgaca attgttgcaa caaggtgttc gacacatcat catcggcgtt
    12181 ggcggaagta gtaccaacga tggcggcatc ggcatggcag aaggtctagg ttatgctttt
    12241 tacgatcaag ataatcaaaa agtagaagca ctgggagaaa atctaggtaa aattgctaca
    12301 gttgataaaa cgaacgtgcc tattgagctg attaaagcca aaatcactgt cattactgat
    12361 gttactaatc ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc acaaaaaggc
    12421 ttgaaagaaa atgaattagc agtggctgac caagccatgc atgattttta tatgagattt
    12481 gcccctcaag taatcgaggc atcgggcagt agtgctggtg gtggaatggg tgcaggactg
    12541 ttagcatttg cttcagggaa aatagtctct ggcatcgact acgtattgga tttactggag
    12601 ttcgataaac gggtacagca agcagacctc gtaatcgtag gcgaaggacg tctagatgga
    12661 caaagtctaa aaggaaaagc gccagttggg attgctagaa gagtaccaga gggcgttcct
    12721 gtgatagcta tttgcggaag tgttggtaaa ggaagcgaaa aagcagcaga tgttggcatt
    12781 tcggccgttt ttccgatcct tcctgcagct gtgcctttac aagaagcttt acagcaagcc
    12841 actgataatc ttcatcgcac agctaaaaac atcgcatctt tacttcaagt cgttcatttt
    12901 caaggaggta atcgatgaaa atcaaagcag cactattcga tttagatggc gttcttgtag
    12961 acacagctcg ttatcattac gaagcgtggc tggttttagc taaccagctg tccattccat
    13021 tcactgagaa agaaaacgag caactgaaag gaatatccag aaccgagtca ctagagcgat
    13081 tgctgtcatt tggaaaaatg gaacagaaat tctcagaaaa agagaaatca gcctttgctg
    13141 agcaaaaaaa caatctatat cttcaagcga ttcaaaaaat ggatgaaaca tccgttttgc
    13201 ctggtgcaat agctgttctt gagtacttga aaaaaacaaa catcaagatc ggattgggat
    13261 cagcaagcaa aaatgcacgc ctcattttag aaaaaacgaa tttgacaagt tattttgatg
    13321 tccttattga cggcacgcaa gtatcaaaag caaaacctga tccagaagtt tttctcaaag
    13381 gagcacagca attgaatgta ccgccaaatg catgcttggt cattgaagat tcagaagctg
    13441 gttgtcaagc agctttggct ggtaacatgc atgtattggg aataggtgaa aacataaatc
    13501 ttccttctgc tgaatatgtg attcctgatt taaccgtttt tgatcaagta cgttcgtttt
    13561 ggcatttatc agaagccgta tcacgtatat aatagtttcc gttttttctg tcacaagcaa
    13621 aacgttatcc tccttttgga aatcaaggta tgatgagaaa aaagggggat tttatatgac
    13681 aaaaaaagca gttttcgcag gcggatgctt ttggtgtatg gtcaaaccat ttgaagagca
    13741 gccagggatc atttctgtga catccggtta tactggcggg catgtaccaa atccaactta
    13801 tgaacaagta acaacaggca caacagggca tacagaagca gtagagatcg aatatgatcc
    13861 agaagttatt tcttatgatc agttggtgga aatctattgg cagcaaaccg atccgactga
    13921 cgcattagga cagttcgctg atcgcggtga ttcttatcgt ccagtgatct actataatga
    13981 tgaagaagaa cgaaatattg cagagcgttc aaaacaagca ttgcaagaca gcgggcgatt
    14041 cgataagcca atcgtgacaa aaatcgaacc aagaagcgaa ttttatcctg cagaagatta
    14101 ccatcaggat tattacaaga aaaacaagct ccattacaat ctttatcgag aaggttctgg
    14161 acgtgcaggt ttccttcgtc gacattgggg cgtcaagaaa taagaagccg aaaaagctac
    14221 ttatggaaca ccgtatatca gaggttgtaa aaccagcatt ttgagctaat actgagggtc
    14281 agctgattga acaccattta gtggagaggt tgtgaacctg tcgcctagct ccaagtcaca
    14341 aggatttggt aagagaacac tatatatcag aggttgtgac aagtcgtttg tcacaacctt
    14401 ttttcaatac ctaagatgaa ggacaatcag caacgcactg ctcaaaaaag gaatgaaagg
    14461 cagcttttcc aacttttttt tatgacagat aaagtaaaca gaaaaaacgg atattccaaa
    14521 cagactagca agaaacaaga tctgaagcaa ctcttctaga gacaagccac cactccaaga
    14581 aagcatcaac agagtatcgc cagctccgag ataggattga ccatagcgaa ggcacacacc
    14641 gcctacgata aacgttaaca gcacactttg ccattggaag acgccggcct gcaaccaaaa
    14701 aatccataaa attgcccaag aacagaatag cgtgtgagaa tggaccatga agtaaaataa
    14761 atccatcaac gaaaatagca aagccgaaag catccatatg aacgtcgaaa aatttcttgc
    14821 ggaagaaaaa gtaaagaacc aggcaaaaag accaccacat aaggcttctg ccaacaaata
    14881 gtagaagggc aaccgacacc tgcaatagcg gcagcgaaat cgttggacga tgatcgagag
    14941 aatcggaatc aattcatacc aagaaagcga acgacagcat ttgacacaat gagatctagg
    15001 ataaataata gagtgaccta acggaatccg ctgggcggtt aaacataaaa atgatccgat
    15061 acagcaacca atgataaaat aaagtaacac gccatctccc ccttttcaaa accaagatac
    15121 gcaagcttaa actaaatttt tgtacataca taaaatttga ggtgaaaaaa tgaacgtttc
    15181 aatcggatta gatatcggaa ccacacaaac caaagcagtt gctttcacag atgaagccca
    15241 agaagcagca tccgcttatt ttcgctaccc tttgatccaa gaaacacaag ggatggcaga
    15301 acaagatcag gaattgatct ttcaaggtgt ctgcaccgtt ataaaagaag taattaaaca
    15361 gttgccttca gacacaatca ttcaatttgt ctccttctcc tctgcgatgc atggcctgat
    15421 attgttggat gaagagaaaa caccgttatc tcgaatgatc acttgggcag ataaccgagc
    15481 agcggacgaa gcagaaaaac tgaaatatac cgtgcttggc aaagaatttt atcggcagac
    15541 tgggacgccg attcatccaa tgacaccatt gaacaaaatc aagtggctgc aaaacagcca
    15601 acctgaatta ttcaaaaaaa ctgcttattt tgtcggtatc aaagactata tcttttatag
    15661 attgtttggc gaactgatca gcgactacgg aacagcttca ggaactggat attttgatat
    15721 ccatgaattc caatgggcag aagaagtgct tgactacttg aacattcgaa tagagcaatt
    15781 gccgcaagtc tttccttcca cctatcaagt aacaggtcta ccatcaaaaa cagcagcaga
    15841 actaggtttg ccaaatgata tatcatttgt tttgggaggg gcagacgggc cgctttctaa
    15901 tctagggtta ggtgcttttg gtgatacgat cgccgctttg accgtaggaa cgagcggagc
    15961 acttcgtttt attactactc agcctcagtt ccatccagaa atggagacgt tttgttatgt
    16021 tctggatcag aaacattggg tagtgggagg agcgactagc aatggtgctg gcatctttga
    16081 ttgggcaggg catacatttt tacaggaagt cgttcaatct gctaaagaaa aaggagaaaa
    16141 tccatatgat gcactgcttt cggctatcgc ttttgtgcca gcgggtgcca atggtctgat
    16201 ttttcatccc tatttacttg gtgagcgagc accgctttgg gacgcagatg cgtttggcag
    16261 ttttatcggt ttacagcggc aacatacaga aaaagaaatg atgaaggcga ttctagaagg
    16321 gatctgtctc aacctttatc ggattttaaa aggagtttgc caggaaaatc agctcacaga
    16381 gatccgtgct acaggcgggt ttgctcaatc tccgattttt cgtcagatga tggctgatgt
    16441 actgggttac ccgcttgttt tccctactgt cacagaagca tccgcattag gagctgtgat
    16501 tttaggctgg agaagcatcg gaaagatcca aagtatcagt gaagcacagg aattgataga
    16561 attgaaagag caagttggag tacaagaaac tgatcataaa aaatatcaga aaatctatcc
    16621 attatttgtt tcgacacaaa agcatttagc acagacttat cagcaatttg ctcaactgcg
    16681 agaagaatta gctgattctg aaaaatgatt ggctttacgg ataaaaaagc gaactgaaaa
    16741 tcgttctcag gtaaatgatt ttgttgtact tttcattgaa agtctactat tattttaagt
    16801 gtaagaaaat gttaggaggc aagaaagatg aaattccaac aaaccaaaga agtattaaat
    16861 caattagttg cagatctaag ccaaatgtct gttgttgttc accaaacaca ttggtacatg
    16921 agaggaccag gtttccttac attgcaccca atgatggatg agttcatgga tgatttgaac
    16981 gctcagttag atgaagtatc cgaacgcttg atcacattag acggttcacc ttattctaca
    17041 ttgcgtgaat ttgcagataa cacaaaaatc cctgatgaaa ttggccgctg ggatcgtact
    17101 attccagaac gtttggaaac acttgttgca ggctatcgtt atctagcaga tctttaccaa
    17161 aaaggaatcg aagtttctgg agaagaaggc gacgatccaa cacaagatat ctttatcgaa
    17221 ttcaaaacag ccacagaaaa acgtatctgg atgatccaag ctcatttggg caaagcgcca
    17281 gagatcgacg cgtaaaaact gttgttgaac ttgaccgaaa aagaggttgt gaaatcagcg
    17341 ttttgacctg agtattagag aaacaaacgt caaaatagtt cctcatattt tgccgttttt
    17401 tgagctaata ccgagggtca gctgattgaa caccgtttac caagaggttg taagcctgcc
    17461 gttcagctcc gagtcacaag aaacaatttt aaaaaacagc ttcttatgtt ttatggatat
    17521 tgtgacttgt gaccgaggag ttggcaggag aacaccgttt attcaaaata aggggttgtg
    17581 acaagacttt tgtcacagcc tctttttgtt tttcattttg acaggaaacg acccttttat
    17641 ataaaagaaa gaaaagaagt aaagtttcat ataaattttt caatcaagca gaaaaacgtt
    17701 gacattgact agattagcgg gtaatatgtt aaatggtatt gtttgcccca caaagagccc
    17761 cggaaactcg aagtgtatgt caaacatcag aacttaaatt ggaggaaaat aacgtgcgta
    17821 caacatatat ggccaaacca ggcgaagtag aacgtaaatg gtatgtagta gacgcaactg
    17881 atgttccatt gggacgtctt tcaacagttg ttgcatctgt actacgtggt aaaaataaac
    17941 caactttcac acctcatttg gatactggcg atttcgtcat cgtcatcaat gcagacaaag
    18001 tgaaattaac aggtaaaaaa gcgactgaca aaatttatta ccgtcacagc aactacccag
    18061 gaggattgaa atcaatcact gctggcgaat tgcgtgctaa aaattctcgt cgtttgatcg
    18121 aaacttctgt aaaaggtatg cttcctaaaa acactttagg ccgcaaacaa tttacaaaat
    18181 tgaacgtata cggtggagca gaacatccac atgcagcaca acaaccagaa gttttagaca
    18241 tcacaaactt aatctaatag gagggaattt cattggcaca agttcaatat atcggcacag
    18301 gccgtcgtaa aaacgcagtt gctcgcgtac gtctagtacc aggaactggc aaaattactg
    18361 taaataaaaa agatgttgaa gaatacattc cacatgctga cttgcgtgaa gtaatcaatc
    18421 aacctttcgc tgttactgaa acaaaaggcg catatgacgt atttgttaac gtaaacggcg
    18481 gaggatacgc tggacaatca ggagctatcc gtcatggtat cgctcgtgca ttgctagaag
    18541 ttgatcctga cttccgttta gctcttaaac gtgctggctt acttactcgt gacgcacgta
    18601 tggtcgaacg taaaaaacca ggtcttaaga aagcacgtaa agcttcacaa ttctcaaaac
    18661 gttaatatcc tttatatatc aacgtttctg gcacttttca attatttgga aagtgcttct
    18721 tttgttttta agaacacatt taagaacaca tatcaatcac acttattcaa tcctctctga
    18781 aaagcttcaa aaacagcctt atcaactata tctggtgtgt tcacatatat ctttgttgta
    18841 gaaatatctg agtgtgttaa agcatttgaa atttgattca tatctgctcc accttgtcta
    18901 gctatagttg cataagtatg acggagtttg tgtggagata aatgaactaa gtcaggatgc
    18961 cttcttttaa ttgaattgat tcgatagttt aggtaatcgg catgaacagg acaatttact
    19021 tctccagatg gtttagtata agtaaagaga tactgatcta acgtttgctt aatttttaat
    19081 ttcaacaatt gatcagcttg taccgatttc cattcactta gcaatgtcat tacaacttct
    19141 ggtacttgaa tgacggtatc ttttcgacct tttgtaaact tttttctttg atatttatct
    19201 aaagtatgtt taagacggac cgtttgtttt tcaaaatcaa tatatttcca ttgcaaagcg
    19261 taagtctcac tttttctatc accaagatac agggtaatca taaaaagtgt gtaatcatga
    19321 aaagtaagta aatgattatt aaaatcatca tttactgctt ctatccaatt agtaagttct
    19381 tttgatgata gtgcttgctt accttcttgt attctttttt cttctaaagc atttttttta
    19441 ggtgcagtga tactttgaat aattttagta gttcgattaa attcgatata atttagtatt
    19501 tctgcaatat cgaacatgga acgaacatat ccttttatga t
//
LOCUS       DACCIQ010000049        19437 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCIQ010000049 DACCIQ010000000
VERSION     DACCIQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.049"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IVM62_001726"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IVM62_001726"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6845691.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IVM62_001727"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IVM62_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6845692.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IVM62_001728"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IVM62_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6845693.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IVM62_001729"
     CDS             2855..3730
                     /locus_tag="IVM62_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6845694.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IVM62_001730"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IVM62_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP6845695.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="IVM62_001731"
     CDS             4627..5061
                     /locus_tag="IVM62_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP6845696.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="IVM62_001732"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="IVM62_001732"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP6845697.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="IVM62_001733"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="IVM62_001733"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP6845698.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="IVM62_001734"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="IVM62_001734"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP6845699.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="IVM62_001735"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="IVM62_001735"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP6845700.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="IVM62_001736"
     CDS             13332..14258
                     /locus_tag="IVM62_001736"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP6845701.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="IVM62_001737"
     CDS             14440..15333
                     /locus_tag="IVM62_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP6845702.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="IVM62_001738"
     CDS             15467..15592
                     /locus_tag="IVM62_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845703.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="IVM62_001739"
     CDS             complement(15726..16358)
                     /locus_tag="IVM62_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845704.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16506..17048)
                     /locus_tag="IVM62_001740"
     CDS             complement(16506..17048)
                     /locus_tag="IVM62_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP6845705.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17183..17572
                     /locus_tag="IVM62_001741"
     CDS             17183..17572
                     /locus_tag="IVM62_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845706.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17576..18340
                     /locus_tag="IVM62_001742"
     CDS             17576..18340
                     /locus_tag="IVM62_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6845707.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18469..19416
                     /locus_tag="IVM62_001743"
     CDS             18469..19416
                     /locus_tag="IVM62_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6845708.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
BASE COUNT         6806 a         3296 c         4115 g         5220 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16381 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16441 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    16501 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    16561 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    16621 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    16681 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    16741 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    16801 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    16861 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    16921 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    16981 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17041 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17101 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17161 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17221 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17281 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17341 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17401 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    17461 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    17521 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    17581 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    17641 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    17701 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    17761 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    17821 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    17881 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    17941 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18001 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18061 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18121 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18181 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18241 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18301 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18361 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18421 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    18481 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    18541 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    18601 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    18661 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    18721 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    18781 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    18841 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    18901 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    18961 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19021 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19081 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19141 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19201 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19261 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19321 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19381 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatat
//
LOCUS       DACCIQ010000050        18162 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCIQ010000050 DACCIQ010000000
VERSION     DACCIQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.050"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(68..1576)
                     /locus_tag="IVM62_001744"
     CDS             complement(68..1576)
                     /locus_tag="IVM62_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP6845709.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1735..1926
                     /locus_tag="IVM62_001745"
     CDS             1735..1926
                     /locus_tag="IVM62_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845710.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2129..3046
                     /locus_tag="IVM62_001746"
     CDS             2129..3046
                     /locus_tag="IVM62_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP6845711.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3039..4112
                     /locus_tag="IVM62_001747"
     CDS             3039..4112
                     /locus_tag="IVM62_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845712.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4260..4778
                     /locus_tag="IVM62_001748"
     CDS             4260..4778
                     /locus_tag="IVM62_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845713.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4759..5151
                     /locus_tag="IVM62_001749"
     CDS             4759..5151
                     /locus_tag="IVM62_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP6845714.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5227..6162
                     /locus_tag="IVM62_001750"
     CDS             5227..6162
                     /locus_tag="IVM62_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP6845715.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6295..7074
                     /locus_tag="IVM62_001751"
     CDS             6295..7074
                     /locus_tag="IVM62_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP6845716.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7067..7384
                     /locus_tag="IVM62_001752"
     CDS             7067..7384
                     /locus_tag="IVM62_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6845717.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7628..9238
                     /locus_tag="IVM62_001753"
     CDS             7628..9238
                     /locus_tag="IVM62_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6845718.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9395..10243
                     /locus_tag="IVM62_001754"
     CDS             9395..10243
                     /locus_tag="IVM62_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6845719.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10474..10953
                     /locus_tag="IVM62_001755"
     CDS             10474..10953
                     /locus_tag="IVM62_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845720.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10986..13214
                     /locus_tag="IVM62_001756"
     CDS             10986..13214
                     /locus_tag="IVM62_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6845721.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13343..14986
                     /locus_tag="IVM62_001757"
     CDS             13343..14986
                     /locus_tag="IVM62_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6845722.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15059..15604)
                     /locus_tag="IVM62_001758"
     CDS             complement(15059..15604)
                     /locus_tag="IVM62_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP6845723.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15627..16112)
                     /locus_tag="IVM62_001759"
     CDS             complement(15627..16112)
                     /locus_tag="IVM62_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6845724.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16102..16914)
                     /locus_tag="IVM62_001760"
     CDS             complement(16102..16914)
                     /locus_tag="IVM62_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP6845725.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17053..>18162)
                     /locus_tag="IVM62_001761"
     CDS             complement(17053..>18162)
                     /locus_tag="IVM62_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6845726.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5901 a         3305 c         3637 g         5319 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtataagtaa
       61 actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga
      121 ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt
      181 gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg
      241 cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa
      301 gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt
      361 catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca
      421 tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag
      481 cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga
      541 ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat
      601 taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac
      661 ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta
      721 cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt
      781 caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt
      841 tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg
      901 ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt
      961 cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagg
     1021 aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg
     1081 tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg
     1141 gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga
     1201 taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata
     1261 agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat
     1321 aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag
     1381 cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta
     1441 cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa ttctaaatat aatttgtcat
     1501 agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa
     1561 aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga
     1621 ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa
     1681 cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa
     1741 aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat
     1801 gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa
     1861 acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt
     1921 aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta
     1981 gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc
     2041 gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa
     2101 aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga
     2161 gccagacgaa ctagaggaga cgaatgcgga tcaggataca ggacaagaat cgtcttcttt
     2221 ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc
     2281 ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg
     2341 attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat
     2401 gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat
     2461 ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg
     2521 tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa
     2581 atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt
     2641 gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca
     2701 aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat
     2761 tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta
     2821 tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc
     2881 agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg
     2941 tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga
     3001 gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag
     3061 aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag
     3121 aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa
     3181 tacacgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt
     3241 acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg
     3301 tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga
     3361 tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac
     3421 caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac
     3481 cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac
     3541 atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac
     3601 cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga
     3661 caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg
     3721 atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag
     3781 cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac
     3841 ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta
     3901 tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag
     3961 tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct
     4021 tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat
     4081 tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat
     4141 tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt
     4201 ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa
     4261 tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga
     4321 tcgcttttca atttattccg aatttccagc caatgactgc tattttttta tttatagtac
     4381 tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt
     4441 tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga
     4501 gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag
     4561 tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt
     4621 tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc
     4681 acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca
     4741 atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt
     4801 atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag
     4861 gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg
     4921 gaaaaagaag tgttcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt
     4981 gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa
     5041 aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag
     5101 atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa
     5161 aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa
     5221 aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct
     5281 aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg
     5341 ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt
     5401 gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa
     5461 tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg
     5521 atggaagctt ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt
     5581 atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa
     5641 gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga
     5701 cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg
     5761 gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc
     5821 ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat
     5881 agtctgatca tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt
     5941 gaacgtcctg agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag
     6001 attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc
     6061 atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac
     6121 attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt
     6181 gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa
     6241 tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac
     6301 aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc
     6361 tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc
     6421 ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct
     6481 atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta
     6541 cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt
     6601 ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt
     6661 tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact
     6721 gcatgggccg gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct
     6781 gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa
     6841 agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta
     6901 ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt
     6961 atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat
     7021 gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata
     7081 tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat
     7141 gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc
     7201 ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga
     7261 tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac
     7321 acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct
     7381 ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta
     7441 tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag
     7501 attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc
     7561 cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg
     7621 aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct
     7681 atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca
     7741 agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct
     7801 gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa
     7861 agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt
     7921 catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag
     7981 tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc catctttgat
     8041 tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc
     8101 ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac
     8161 ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt
     8221 cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa
     8281 agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg
     8341 atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat
     8401 tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc
     8461 agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa
     8521 attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt
     8581 aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc
     8641 gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact
     8701 atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct
     8761 agcttctttg tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca
     8821 ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt
     8881 aatcggatta ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg
     8941 tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc
     9001 aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg
     9061 ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat
     9121 gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg
     9181 aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt
     9241 taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt
     9301 ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc
     9361 tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag
     9421 ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc
     9481 tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta
     9541 tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt
     9601 tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct
     9661 ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg
     9721 agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac acatggtaga
     9781 agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga
     9841 tcgaatccga gataatacct acgcttatct gattgtagat gatttgaaca atttagtacg
     9901 aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat
     9961 cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc
    10021 atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa
    10081 actttacgtg atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca
    10141 gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca
    10201 tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa
    10261 acataccaaa aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta
    10321 aaagaacgag atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac
    10381 ttcaatcctc tataatgtcg catatagttt agtaaatgag taaactacta atttacttat
    10441 ttactaaaaa taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat
    10501 aaaccagtaa tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatacg
    10561 gatgcaaaag ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc
    10621 tcaggcaagg tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca
    10681 atcacacgta ttttagattt agcgatctta gctgcacaag gaagtttata cttccaagat
    10741 gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta
    10801 caagggaacc gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc
    10861 attttgtatt acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt
    10921 ttaaaacgtc ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga
    10981 gaagcatgga gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa
    11041 tgatctggaa gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg
    11101 aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag
    11161 atgccgggtc ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct
    11221 atacaaaagt gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac
    11281 ctgtagtaga ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag
    11341 atacacttga agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg
    11401 ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc
    11461 attacagcta ttacccttct gaaatcgttc gagcgattga aaaaggatat ttattagaga
    11521 ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg
    11581 aaccaaatgg gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca
    11641 ttgtgatcac tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag
    11701 atcggatgca acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg
    11761 cgatggcgaa aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc
    11821 gtttgttgga tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt
    11881 atttttattg ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa
    11941 aagttttgta taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt
    12001 ctgacagtgg attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc
    12061 ctcaatctga gcatacaaaa ggaagaacca ttagcgccga agaagcaaat gaacgaaaca
    12121 tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag
    12181 aaccaaatga gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc
    12241 aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag
    12301 gagatgattc tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga
    12361 agcaactgaa taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc
    12421 tcatcgttca ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc
    12481 aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac
    12541 aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag
    12601 catatggtga tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag
    12661 cggtagctgt acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg
    12721 caaaaaaagc agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga
    12781 tttacggaga tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag
    12841 aatttgaaga cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa
    12901 acaatcgtga tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa
    12961 caaccaaact gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag
    13021 gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc
    13081 tatttgtctc cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa
    13141 gtcgttttgt agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg
    13201 cccgctatct atgataaaat aaagctcaac gaacgcactt agcagattcg ttgagctttt
    13261 cgtttctagt taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg
    13321 accgttaaag gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg
    13381 ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa
    13441 tgggatccgt gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa
    13501 tccgatctat caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat
    13561 agaagagaga ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt
    13621 agggatcaaa gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt
    13681 tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg
    13741 ggtagatggc aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta
    13801 ttctcatgaa actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa
    13861 ttgggaaagc gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa
    13921 agggatagat ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt
    13981 aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt
    14041 gaccgatttg aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg
    14101 tgtcactgca gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt
    14161 cgaatttgat catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa
    14221 attgaaactt gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt
    14281 atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga
    14341 tttttgggct atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc
    14401 atttatttat caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca
    14461 agttgatgca gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag
    14521 agaagaagca ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca
    14581 atggacttcg gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc
    14641 aaataccaaa acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt
    14701 ctataagaac atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa
    14761 agaatatctt catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca
    14821 atatcttatt atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc
    14881 agatcagtcc tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg
    14941 catactagca ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa
    15001 agttcattag ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc
    15061 aataattcgc tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag
    15121 cttgtccaag ggttttcaaa cgaggtctga caggtacttg gacatgtttg atatgcatac
    15181 cgattgatgt gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag
    15241 gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa
    15301 caacagagac aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc
    15361 ggttcaaatg ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt
    15421 ttaacgtacg gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc
    15481 caacgacttc gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct
    15541 cagccaatat gtcattaact atttcagtca attggtcttt taattctttt tctgacaact
    15601 tcattttcta aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga
    15661 cttgcagtta taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt
    15721 atagaagcca ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg
    15781 atcataacta agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg
    15841 aaagaaccag caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc
    15901 ggatatcctg aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc
    15961 gttggaccat aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca
    16021 ttcgtagccg gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc
    16081 atagcataga tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc
    16141 agaccagaca cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac
    16201 aaatttttta ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt
    16261 tattgcagaa gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg
    16321 agaaaatacc tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat
    16381 ccttgtgccg cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag
    16441 atggatcatt tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc
    16501 caaattaggc gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata
    16561 caattgattg atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg
    16621 gatattttgt tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa
    16681 taacccaatt ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt
    16741 aagcaattcc aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat
    16801 agaagtcaag gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg
    16861 gattcctcca ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt
    16921 ttcctccatg aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata
    16981 tttttatctc atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct
    17041 ttccttaaat agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac
    17101 tttcattgta gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg
    17161 aggaagttca tggacttgct cccaccatcg attccaatcc atttctttat caggagctaa
    17221 agcaataatc ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa
    17281 atctaacgaa tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa
    17341 atccacttgc cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc
    17401 gctatcccac caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt
    17461 caaagcccgt cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt
    17521 cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct
    17581 tgcatcactt gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc
    17641 actttggcca tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt
    17701 cttcattgtc tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa
    17761 agcttctgga tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc
    17821 tagtgcaatt ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc
    17881 taaaggcatc ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga
    17941 atgcactgga gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt
    18001 tttttgccat ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat
    18061 accagttgta ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg
    18121 agaaacagac aaagcaggtt gttccattaa ataaagaata gg
//
LOCUS       DACCIQ010000051        16697 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCIQ010000051 DACCIQ010000000
VERSION     DACCIQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.051"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(354..719)
                     /locus_tag="IVM62_001762"
     CDS             complement(354..719)
                     /locus_tag="IVM62_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845727.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            927..1409
                     /locus_tag="IVM62_001763"
     CDS             927..1409
                     /locus_tag="IVM62_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845728.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1606..2496
                     /gene="pstS"
                     /locus_tag="IVM62_001764"
     CDS             1606..2496
                     /gene="pstS"
                     /locus_tag="IVM62_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP6845729.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2821..5292
                     /locus_tag="IVM62_001765"
     CDS             2821..5292
                     /locus_tag="IVM62_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6845730.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5395..7131)
                     /locus_tag="IVM62_001766"
     CDS             complement(5395..7131)
                     /locus_tag="IVM62_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP6845731.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7128..7832)
                     /locus_tag="IVM62_001767"
     CDS             complement(7128..7832)
                     /locus_tag="IVM62_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6845732.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7963..8466
                     /locus_tag="IVM62_001768"
     CDS             7963..8466
                     /locus_tag="IVM62_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP6845733.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8426..8764
                     /locus_tag="IVM62_001769"
     CDS             8426..8764
                     /locus_tag="IVM62_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP6845734.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8785..10203
                     /gene="ffh"
                     /locus_tag="IVM62_001770"
     CDS             8785..10203
                     /gene="ffh"
                     /locus_tag="IVM62_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP6845735.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10315..10893
                     /locus_tag="IVM62_001771"
     CDS             10315..10893
                     /locus_tag="IVM62_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP6845736.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10897..11418
                     /locus_tag="IVM62_001772"
     CDS             10897..11418
                     /locus_tag="IVM62_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6845737.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11497..12051)
                     /locus_tag="IVM62_001773"
     CDS             complement(11497..12051)
                     /locus_tag="IVM62_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6845738.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12304..12579
                     /gene="rpsP"
                     /locus_tag="IVM62_001774"
     CDS             12304..12579
                     /gene="rpsP"
                     /locus_tag="IVM62_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP6845739.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12591..12842
                     /locus_tag="IVM62_001775"
     CDS             12591..12842
                     /locus_tag="IVM62_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6845740.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12967..13758
                     /locus_tag="IVM62_001776"
     CDS             12967..13758
                     /locus_tag="IVM62_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845741.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13928..14449
                     /gene="rimM"
                     /locus_tag="IVM62_001777"
     CDS             13928..14449
                     /gene="rimM"
                     /locus_tag="IVM62_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP6845742.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14439..15200
                     /gene="trmD"
                     /locus_tag="IVM62_001778"
     CDS             14439..15200
                     /gene="trmD"
                     /locus_tag="IVM62_001778"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP6845743.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15336..15683
                     /gene="rplS"
                     /locus_tag="IVM62_001779"
     CDS             15336..15683
                     /gene="rplS"
                     /locus_tag="IVM62_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP6845744.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15941..16234
                     /locus_tag="IVM62_001780"
     CDS             15941..16234
                     /locus_tag="IVM62_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP6845745.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16322..16435)
                     /locus_tag="IVM62_001781"
     CDS             complement(16322..16435)
                     /locus_tag="IVM62_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAP6845746.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         5622 a         2950 c         3400 g         4725 t
ORIGIN      
        1 ttaattagtt tactttcttc aacactttat tctttgaata ttatagatgt ttccacctca
       61 aattcaatat ctattattgg aatcattatt gctgcaatta ttacacaact gtttttagtg
      121 tttggagaga agaaaagaag taaaaaatag aaaaactagt gatagttttg gtgatagtga
      181 tattaaaaat cagaaaagat agaaaaagca ggagatagat aaaatgctga tatttcagca
      241 ttttttaaga gtataaaaga gaaaaaaagt aataaatgta tacatgatat aagaaaaaca
      301 tatatgtata aaaagaagaa agtagcttct ctttgtacat atatggtttg tttttatgga
      361 tgccgagctt ttttataaga taaggctacc gcccataacg acccaaggat ttgtggaact
      421 aagtaaatac ctgcaagaag caagatcagc tgggtcggtt ttaaaggatg atagaagtaa
      481 cgccattcag taaaattggc gcaaaataaa taggaagtga cagtagatat aatattggca
      541 atgaataaag atataatacg cctgttcctt tgaaaataat atccagcaaa aagagataaa
      601 aatatcaatg tgatgaaccc gcttacggaa tgataacgaa aatcaagcag taaggcgaaa
      661 tgcacataag gaacaaaaga taaaagcaac ggaaggtttt tccagcaagt ttttatcatc
      721 tttcatcacc acctaacaag tgttattatt ttgttagttt tattgtaaat tgaatcctgc
      781 aataatcaat tactttttca ttatttctag attaaatgaa taaactctca tgccattatc
      841 tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata gtatgatgag
      901 agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat cggctttatt
      961 tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat gctgttgtac
     1021 aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct aggaattttc
     1081 cagctttgac tgttttctat ttagtgataa gtgtaggtct cttattacca aaatctctga
     1141 tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt acatctatgt
     1201 tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt ggattgaagg
     1261 attcagctta ttcctccatg tatttcttga cgatcaaacc atggttctat ttagtaattg
     1321 cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata aaaagtgaac
     1381 gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa gcatatgagc
     1441 tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa tgcgaaaaac
     1501 gtttacatga tttttacata acttaactat atttttacag caaccatacg agggatttac
     1561 agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg
     1621 tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc aggaaataca
     1681 gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa aatcgttgcg
     1741 gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt tgtacaagaa
     1801 aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt gagccaagtc
     1861 gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag agacggagtt
     1921 gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcgcc aatcgtcaat
     1981 aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt tacaggaaaa
     2041 atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca
     2101 aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc tacccctgtg
     2161 cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca aacaccagga
     2221 gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact aagtatcgac
     2281 ggtgtagaac caaaagaaga gaatgtagca gataacagct ggggaatctg gtcatatgaa
     2341 catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga ctacatgatg
     2401 acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat cacaatgatg
     2461 gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca tgccacggta
     2521 gtcgttacta ttaaggaaaa atcaactatg tttcccacat atacattttt cctttaattg
     2581 tgaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgatta
     2641 aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtagctc tttttttgct
     2701 atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt
     2761 ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac
     2821 atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat
     2881 aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa
     2941 cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa
     3001 atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg
     3061 ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat agccaataca
     3121 ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta
     3181 aatacagcaa aagtcaatgt catacgggac gggatagaac aaacaataga tatagaagac
     3241 cttgtaatag atgacattgt tattttagag actggacatc aaattccagc agatgcagag
     3301 gtcgtggaag gaaaactgca agttaatgag gctttattga caggcgaagc agatgaaata
     3361 accaaagaaa taggcgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac
     3421 gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag
     3481 acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg
     3541 attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat
     3601 ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct
     3661 gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag
     3721 caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt
     3781 tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc
     3841 tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat
     3901 gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa
     3961 accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt
     4021 gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa
     4081 ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc
     4141 tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta
     4201 ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc
     4261 aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat
     4321 gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca
     4381 gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa
     4441 caaaagaaaa tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat
     4501 ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga
     4561 aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg
     4621 ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg
     4681 tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg
     4741 acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca
     4801 tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat
     4861 gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg
     4921 atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt
     4981 ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga
     5041 agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag
     5101 ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc
     5161 tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa
     5221 aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa
     5281 cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc
     5341 tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat
     5401 tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt
     5461 accattaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga
     5521 atgtcggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc
     5581 aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata
     5641 gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt
     5701 gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg
     5761 aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata
     5821 aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat
     5881 gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga
     5941 agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag
     6001 tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt
     6061 ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc
     6121 tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat
     6181 ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg
     6241 ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag
     6301 ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc
     6361 aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata
     6421 catcttactc atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc
     6481 gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg
     6541 cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag
     6601 aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg
     6661 taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt
     6721 gactggtaat gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct
     6781 acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt
     6841 caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg
     6901 ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt
     6961 ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg
     7021 ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact
     7081 aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt
     7141 tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct
     7201 cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg
     7261 aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt
     7321 ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct
     7381 acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc
     7441 tcagcttcat cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg
     7501 gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg
     7561 ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt
     7621 ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct
     7681 aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg
     7741 gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa
     7801 ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc
     7861 tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta
     7921 taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca
     7981 tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg
     8041 gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca
     8101 attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa
     8161 gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc
     8221 aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc
     8281 ccttcatgcg gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt
     8341 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     8401 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     8461 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     8521 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     8581 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct
     8641 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     8701 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     8761 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     8821 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     8881 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     8941 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     9001 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     9061 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     9121 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     9181 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     9241 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     9301 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     9361 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     9421 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     9481 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     9541 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     9601 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     9661 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac
     9721 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     9781 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     9841 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     9901 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     9961 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
    10021 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
    10081 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
    10141 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
    10201 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
    10261 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
    10321 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
    10381 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
    10441 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
    10501 gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
    10561 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
    10621 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
    10681 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
    10741 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
    10801 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
    10861 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
    10921 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
    10981 actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc
    11041 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
    11101 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
    11161 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
    11221 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
    11281 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
    11341 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
    11401 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
    11461 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
    11521 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
    11581 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
    11641 cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc
    11701 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
    11761 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
    11821 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
    11881 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
    11941 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
    12001 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
    12061 cctccttcaa actatctata ataatcttac aaaagataag taactggagc aaataaaaac
    12121 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
    12181 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
    12241 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
    12301 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
    12361 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
    12421 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
    12481 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
    12541 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
    12601 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    12661 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    12721 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    12781 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    12841 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga
    12901 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    12961 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    13021 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    13081 agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca
    13141 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    13201 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    13261 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    13321 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    13381 acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt
    13441 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    13501 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    13561 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    13621 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    13681 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    13741 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    13801 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    13861 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    13921 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    13981 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    14041 gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    14101 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    14161 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    14221 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    14281 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    14341 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    14401 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    14461 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    14521 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    14581 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    14641 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    14701 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    14761 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    14821 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    14881 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    14941 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    15001 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    15061 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    15121 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    15181 cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt
    15241 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag
    15301 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    15361 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    15421 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    15481 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    15541 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    15601 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    15661 gtatcaaaga aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc
    15721 aagggttctt ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta
    15781 ttggtagggg agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga
    15841 gcactatgat aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta
    15901 acataaatgg agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga
    15961 gcaagaaaag atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc
    16021 aagttcaaca aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg
    16081 agtgacttat acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga
    16141 ttatgtcgaa caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg
    16201 tcgggcatat cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa
    16261 aagttcaagt atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta
    16321 tctataatac aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg
    16381 agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa
    16441 aatcaattgg atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac
    16501 caaaacgtac ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt
    16561 tcgttcttga aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt
    16621 cctgagcgtt agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt
    16681 gaatacaaga tcagcta
//
LOCUS       DACCIQ010000052        16639 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCIQ010000052 DACCIQ010000000
VERSION     DACCIQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.052"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            44..265
                     /locus_tag="IVM62_001782"
     CDS             44..265
                     /locus_tag="IVM62_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP6845747.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            267..533
                     /locus_tag="IVM62_001783"
     CDS             267..533
                     /locus_tag="IVM62_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6845748.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            646..1185
                     /locus_tag="IVM62_001784"
     CDS             646..1185
                     /locus_tag="IVM62_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845749.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            1679..1963
                     /locus_tag="IVM62_001785"
     CDS             1679..1963
                     /locus_tag="IVM62_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845750.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            2237..2491
                     /locus_tag="IVM62_001786"
     CDS             2237..2491
                     /locus_tag="IVM62_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845751.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            2573..2758
                     /locus_tag="IVM62_001787"
     CDS             2573..2758
                     /locus_tag="IVM62_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP6845752.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            2825..3220
                     /locus_tag="IVM62_001788"
     CDS             2825..3220
                     /locus_tag="IVM62_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP6845753.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            3210..3587
                     /locus_tag="IVM62_001789"
     CDS             3210..3587
                     /locus_tag="IVM62_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845754.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            3588..3983
                     /locus_tag="IVM62_001790"
     CDS             3588..3983
                     /locus_tag="IVM62_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845755.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(4289..4687)
                     /locus_tag="IVM62_001791"
     CDS             complement(4289..4687)
                     /locus_tag="IVM62_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP6845756.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(4877..5644)
                     /locus_tag="IVM62_001792"
     CDS             complement(4877..5644)
                     /locus_tag="IVM62_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6845757.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(5679..6902)
                     /locus_tag="IVM62_001793"
     CDS             complement(5679..6902)
                     /locus_tag="IVM62_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP6845758.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            7883..8686
                     /locus_tag="IVM62_001794"
     CDS             7883..8686
                     /locus_tag="IVM62_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6845759.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            9271..9726
                     /locus_tag="IVM62_001795"
     CDS             9271..9726
                     /locus_tag="IVM62_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845760.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            9845..10903
                     /locus_tag="IVM62_001796"
     CDS             9845..10903
                     /locus_tag="IVM62_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP6845761.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            10945..12894
                     /locus_tag="IVM62_001797"
     CDS             10945..12894
                     /locus_tag="IVM62_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6845762.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            12897..16538
                     /locus_tag="IVM62_001798"
     CDS             12897..16538
                     /locus_tag="IVM62_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845763.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         5854 a         2565 c         3097 g         5123 t
ORIGIN      
        1 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
       61 tcgtgtatct aaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
      121 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
      181 cacctacaag gaactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
      241 tatgctaaaa gagttaggct tataagatga cttaccaagt aagatatgaa cgtgaagctc
      301 aaaaagcgct aaaaaaaatg gataagtttc aggcaaaggt tatcctaaac tggatcgaga
      361 aaaatcttgt aggcactgat gatccaagaa aacatggtaa aggtctaaca gctaataaat
      421 caggctactg gcgatatagg gtaggtgcct accgtataat agctgatatt agcgagcatg
      481 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
      541 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
      601 ttacctaaaa aagaacaata ccctcttgtt attatacaca ctggtgtgta taatataagt
      661 gtaaacaagc taattaactt tcaaaaggag cgatttttta tgggatggta tggtgcttat
      721 tcatcagcaa atgaagtgat cgaagaaaag aaagggtacg ccaaaaatgg tttggaaatc
      781 gtagatacca aagtaacgaa gacctatggt attatccttt tcaaagatgc agaaggaacc
      841 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
      901 gacagtcctg cttactttaa gaaaattccg aaaaaatggt tgaaattctt tgtcggtgat
      961 caagaaaaaa tggttaagca acaactagat agaattaaag aagaaaacaa taaaccaaac
     1021 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     1081 gtttatcagt ataaactcaa acgtagcttt atttttagac tacaagatgg atcactcact
     1141 cggttcactc gactatcagc aggagatgtt aaggaagtag cctaaaactt aaatacataa
     1201 acataaacgt gtatacgttt atgtttatgt ttatgtatat gtatatgaat ataaacgtat
     1261 taggtacgaa cgtttttaaa tgcttttctg ggctttgatt ttagggtagg gctaaaacat
     1321 ccattgatcc aaaacagctc agaaacgcat ttaaacgtat ctgataccaa tattacagaa
     1381 ggagtgtatc gtatgattaa cctatttctt ttagtgacag ctagtacatt attctttact
     1441 ggaatatcac tgataatttc ctatttgatc tataagaaag ccgaaactta cacaagcaaa
     1501 gcaaccggac tagccgtttc ctccttttta actttgctga ctttttttgt tactctatcg
     1561 ttttcttttg tttagcacag ctgatccatc agcaaaaaac ctctctttac attatacaca
     1621 ctagtgtgta taatgtaatt gtaaacaagc taattaactt ttaggaagga gtgacaatat
     1681 gctaacagct acattacagc aacctactca cgttgctatt gcagaaagct atacaattaa
     1741 tgcaggggtc gttaaacagc tgatcaagga tcgagaattc aaaaagttta acagtcgtca
     1801 agcgtataac agacagttaa agaaagctat tctaaagcac aaaaattcct tcatagggaa
     1861 attggaaagt gtggatgtta aaactgtttc aacggcattt tcagttgttg aagtgactat
     1921 tgacggtcgc ttgatttctc tatttgcttt tggaattaat tgatagggag tgtggacttt
     1981 ggaaggtaag acttttgcag aaatttggac tgaatttatt gaggtaccaa ctcgcattga
     2041 tccgataact tctgaaaagg tattggatgc agacttctac aatctcaaag caggtactag
     2101 ccttgaatgc gtttagatgt atttagaaga gcatttcaaa ggcgtaacgc tcggtcacgt
     2161 cttataccac aatggtgccg agtgggatgc aattggaaat atccattttt cttaatacaa
     2221 gaaaggagaa cacaatatga attataccga tgaagaacaa atcactttgg aatacgtttt
     2281 cacaaatgaa aaaggggagg aaattgaaaa agtcttgatc atttcatcaa aagacttgga
     2341 gaacgctttg acttatgaac catacgaatc aaccgtaact gtcaatgaat tcttagatac
     2401 ctatacttct aatgatactt gggaaattat gaatatttgc aacgctaata acattcaatt
     2461 tgctgaaaaa accaataaat cattttatta gttttgcaat aaaacatcct ctttttctat
     2521 tgattccata cacaacaatg tgtataatag attttgtaaa gggagggtag ctatgcctaa
     2581 gacagcaaga gaaattgtaa aaattctcaa atctaacggc ttcaaaaaga ctggacaaac
     2641 tggcagtcat gcaaaatttt ataattccaa gactaaaaga acggtcgttg ttccgatcca
     2701 caatggtgat gtaccactag gaacagaagc taatatctgg aagcaagcag ggctttaaca
     2761 gccttgcctt cctatttggt attaggcata ctatcctctc ttacactaga aaaggagcga
     2821 ggaaatgaag acaaaaaata ttttttatcc ggcaatcttc gagaaagaag gagaagctta
     2881 taacatcgtt ttccctgact taccggaaat atctaccttt ggaaatagtc ttgaagaagc
     2941 atatttaaac gctaaggatg ctttaggatt agcgctatat agtgtaccag atgcagaatt
     3001 tcctaaacca tcagcagtgg aagaattaac tttgaaagcc aatcaagtag tagtaatcat
     3061 ccaactaaac attaaacttt tccgaagaag cttaaataca aaaacaatta gaaaaaatgt
     3121 tagtgttccg gaatggttag ttcttttagg gaaggagaaa aacataaact tctctcaatt
     3181 acttcaaaaa gcactagaag aagacttgct tgaaaagtaa aaaggagacc gaacctatga
     3241 aatctacaca cttaaactat ttctctcgaa cgttaatcaa taatctatta aaagatacaa
     3301 ctggcattag cgttagtacc aaacactttg ccaatgatct aattattttt gaggataata
     3361 acttgcgaat tgagatgttt attgaacagg caactcctaa ctcaatacct gaattctgct
     3421 atacgcacac tgaaaaaatg aaacatgcaa aatcagttaa agatgttgaa gcattaagtg
     3481 aatcactaac gaacgcttta aacctcgtta agaaggctaa attgttattt gaatttcaaa
     3541 ccgactacca caatgagttg cttgctcaat ctgaaatttt agattaattg aaacaggaga
     3601 agagaaagat gaaacaaaca caaacagaac ccattaacga tacgcttgct gatcaattag
     3661 caggttatta taaattattt gagatgagta aagcacagcg cttgatccac tatctttgga
     3721 aatacaaaat tttgtttctt ttttgggaag caacactaac attctcagtt cttttttata
     3781 agtcagattt ttataatcaa cacttagttt tagggagttt tttgcttatc tacaccattt
     3841 tgatgcttaa tttatatacc tacatcacac ttgaagaaca cttagtaatt agcgatgcag
     3901 ggatcgagag aaacatcaaa agggtatctt tcaagatcac gttaaacaag cctaccgata
     3961 aaaagctctc agaagcgttc tgagagcttt tgacattttc ttgtataatt tatcctaaca
     4021 cattacaaag cacctaattc gagcttatat caagcaaatt tgctatttat tcctcaatac
     4081 atatctaaaa attacttgat ggaaaactag atacatggta agcatgaaaa gaaaatcaat
     4141 ttgagcagat gcagtataat acatagcaat ttaaaagctc tcagaacgct tctgagagct
     4201 tttaatatat tctttgataa tttgtctttc ttaaatccat acgtcctaaa acaagctgtt
     4261 tttgcttaat atgatcatct gagagacatc aaatcaactt ttctctcaac aaatcattca
     4321 aagctttcga cagattaata ttctcttcag ccgctaaaac atttaaatag tgttccacag
     4381 tcactgattt tcgatcataa ttttgcttgt ttcgagcttt agcaactttc gtattagctt
     4441 ttacgacaaa taaattaaca ttctcagatc gcttgattct tgcatttaac tcttgaacag
     4501 gcgtaggcga tggaatttct ttttcttgca actctaaaag caccaagtat ccttccaata
     4561 cttcttgcgc catctcaaaa gcatcttcta aagtttcacc atgcgtatat aagtctggaa
     4621 aatctgggaa tttaactgca tattcatttc ctttccattc gaatactgca caaaaaaagt
     4681 atttcatcgc attgcacctc tctaattaaa aaataatttt tgctttttgg tttcaaaaaa
     4741 agaatcgcct accaaaaaat tgataggcga tcacaagcta attaacctct aaaggagatt
     4801 aataccttgc tcccaaggca ctacatatct taacatacgt ctgaagcatc ttcaaacgag
     4861 taagatatat tttgcattaa cctaattttt tttctctata aggtttttca tcaaactcac
     4921 tagaaatatt aaactggaca gcaaaattgt aatttctgcc ttttctaact ttagcaaaat
     4981 caatcttcag cattgatttt tcattaattt cttcaactga ttcccttaaa acacgtctat
     5041 caaaattcga tattgtttta tactcatttt ctgttttcgt aatctcacga attttaacta
     5101 tttcaatcag gggattctta tatatttcag ctttctcaaa atctccttta tttttggcga
     5161 taagatattt tttataatac atcgccaaat atttgtataa cacgatgcta tatttttttt
     5221 gaaaagatgc taagtcttta aaaacaaaga acggatactt ctttttgatt gagacaatta
     5281 agggaattac atcttctgaa aaactgaaag atacactatg ctcttctaaa tcccaatgaa
     5341 tccgatcaat agcagataca gtagtatacg tcttatattt cttgtctaga ttcgatattt
     5401 cataaactgc ttgtctttgc aaagtgttaa ctatggtttt tagttccgtt tcaagatttt
     5461 gatcaacatc catgtaatta ataatcgctt taacaggtaa atctactacg cctgttttct
     5521 ttagctttgt gggtgagata taactgaaca gcaattgata aagttcatac gactttaaat
     5581 ccattttcat atcaattgtt gctaagaact cctcattgta ctttgtcagt tcatcttttt
     5641 tcaaaagttt ttccttcctt cacgcatttt ttcagttgtt aatcataaag gttcacataa
     5701 ggcaaataac tcgtaattga tttttcgaga tatttaacag tattttttat tgtgtaatcc
     5761 tccttatgag tagcaacgta agataggtga ttgacaactg attcaaatcc tctatctttg
     5821 gataaacgat cataaagcgg ataaacttta ttctgaagtc ggctcataag tttaagatcg
     5881 cataagtcgg cagaactgat caaaaagttt ttgattagta atttagtgta tttgctattc
     5941 atagctttca agtacgattg attcattttc tctgttttat cttctgcgcc ttttaaataa
     6001 gcagggtctc tttgttcttg cttataaggt accggaactg acatggtacg tgtaagcaag
     6061 aactttatgt ttgtataagt atgacccttc ttgataagct catagtcaat tttaaaatca
     6121 gtgtatttat tcagttgggc aatcggttct ttaattacac gtctttcaaa gtctcgaaaa
     6181 gctttatatt ttttctcaat actaaatttc tttcgtaaat ctgcaatgga gaaaacagga
     6241 ttttttaatc tatcaagtga ttttgtactc catgttaagt tatctctatt tgcttcatac
     6301 agattaaaat ctttagtgaa taattgaaaa aaactgatcg catacttgct tgtcaagtta
     6361 actacttgat caaatgtata ttgcgtaaaa tgatcttcta agttcgttaa taaaggcata
     6421 attgaactac taaatttaat agatactgca tcagaaaaat cattgaattc agtaatttct
     6481 agcggtgaga tgactttgta ttcagtctta ccgttaggtt tagttctttg aattttaatg
     6541 acagattgct gatgcaattt ttcaataact tgttttaatc ttgaatgttt tgaagaagat
     6601 gaactagaag acatcaacgc aaataaagcc tttttagata ctttgacggt attattaaaa
     6661 ggcggatttt tagggtcaag atgtcctata gcgtaaagat aaattttttg tggcatagca
     6721 tccatttcaa caatgctatc tacaagttga ttcgataaag taacagttaa atcaaacttt
     6781 tttagattgc tttcggtagt ttcaaatttc gggtttacat tcttttctgt tattttcata
     6841 cgattgcctc ttctttctaa gcttggtacg agcttaaaaa cttggaactg gtacgattcc
     6901 attgaataag tatagacctg ttttcgatac gttacaataa gaaatgtatc gtaaatttat
     6961 aacataactc aatgtcagta aaacacattt tattttttcc aatcaaattt cattgaaaat
     7021 acagcagaaa tcgctatttt agaacatttt caaaataaaa atccttttca ataaaatacg
     7081 aaattataaa aattaccaaa attaaaaaac aaatattttt ctagttttta gaaataaatt
     7141 cacatacttt ttcttttttt ggatatattc ggaataccac atcgcttttt gtccacgttt
     7201 gcatcgcttt ttgtccacat tcacatcgct ttttgtccat gttcgcatcg ctttatgtcc
     7261 accctaaaat ctatttaaga ttgatataaa gcctttttgg aggttgctaa agttttaaag
     7321 attaaaatat atattataaa tataaataaa aggggaagct gaagcttcct ccttttcctt
     7381 tttttcaatt attcaaagtt acacaagcaa aataaactcc tagatagtgt tagttttttt
     7441 taagattttt tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata
     7501 tctgttaata tatctgttaa atatctgtta atatatctgt taggggaggc ttaatctctt
     7561 gggagagtaa gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa
     7621 gtggacagta agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta
     7681 tcacactagg ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa
     7741 attagattag taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa
     7801 caagcagaaa aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa
     7861 aaagaagatt ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga
     7921 tgtagattta gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac
     7981 aacagctttg cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa
     8041 aatgaaacgt caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt
     8101 caaatacgac agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa
     8161 actaggtaat ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt
     8221 tcaaaaattc cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt
     8281 tgaatttatc ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac
     8341 aaaactaaga agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa
     8401 agagtggcga aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat
     8461 accttcaagt tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga
     8521 atttttagct cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat
     8581 ctatgcaaag aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc
     8641 aaacttgcca accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg
     8701 taattttaat acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg
     8761 caagaagttt ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct
     8821 atacaaattt taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt
     8881 tcttaatttg ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg
     8941 tgatatcagt tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt
     9001 ggacaagtca actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc
     9061 gtctgataaa tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa
     9121 atagctaaaa taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg
     9181 ctgattgatt tatttatgtt attatgatat tataataaca taaatgtgtt acgcccgaac
     9241 ctcattttaa aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt
     9301 aactatatgg agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag
     9361 catgggttac cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt
     9421 attaagctga gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat
     9481 gaactaaaag gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt
     9541 attggaaaat atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct
     9601 actccaatgg ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca
     9661 atagcaaaaa tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga
     9721 agataatttt tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg
     9781 aacctagaat taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa
     9841 actaatggaa aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg
     9901 gttgcttggt gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt
     9961 tctaggagtg ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta
    10021 taattattat ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc
    10081 aggagcagag atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt
    10141 aaaactcggt tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa
    10201 cttatatatg gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat
    10261 gttaagtact gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta
    10321 tcaagttttt attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga
    10381 taccaaagtc aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat
    10441 gaatgatgtt aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac
    10501 tcctgataat atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg
    10561 aagctttctt accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa
    10621 gaataatgtt gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa
    10681 aattgaagct acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga
    10741 ttcgcaagaa agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa
    10801 tgatacacaa aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg
    10861 agacatgaag acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat
    10921 tttagaggaa gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa
    10981 actgaattta atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt
    11041 gatgacgaac tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc
    11101 ttgttaggtg aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag
    11161 cgacaagatg gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg
    11221 ggagatgatg tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag
    11281 cgaattttaa aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat
    11341 aaagttgtta gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg
    11401 ttagcaaatg aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat
    11461 atcaaaagag atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa
    11521 agcgatgttg tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat
    11581 cctcaggctt cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta
    11641 caagcaaaca agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg
    11701 gaggatttga ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg
    11761 caaatatttg gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa
    11821 gaggaattaa agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt
    11881 aaacgtatga agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct
    11941 ctgtttacag gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga
    12001 ctttatggcg atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat
    12061 gatgcaatgc gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt
    12121 attgtgtgtg atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt
    12181 ttagatgaag gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac
    12241 actgtttcat tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc
    12301 gattcgttaa acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa
    12361 tatagcactg aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg
    12421 attggtgaag gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg
    12481 gctttatccg aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa
    12541 ttagtacgaa aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat
    12601 ggtcaaaatt tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa
    12661 gaggattcta aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa
    12721 gaagcgatta ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg
    12781 agtgaagccg gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct
    12841 tccgttgctg aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga
    12901 aattcaagaa atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag
    12961 ttattggagg agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc
    13021 ctgacaatca agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa
    13081 gtaaaccggc agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag
    13141 aagttaatga tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca
    13201 gtgccgattt cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg
    13261 caggtactca actagcatct actttagcaa cttatagtta ctacggatat attgaaacag
    13321 tctcaggaaa tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga
    13381 ttggtagttt tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg
    13441 aactccttgg aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag
    13501 gtggatcgga tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg
    13561 gattaaataa tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa
    13621 tactaggtta tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat
    13681 caccagtact tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg
    13741 caggatcgct attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata
    13801 taccaacaaa tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa
    13861 atcttgctcc atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa
    13921 tcaagttcgt tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca
    13981 atactagcag ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc
    14041 tattaaaaga atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta
    14101 atgtcgccga aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt
    14161 taggggatgg aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt
    14221 ggtcagcaaa tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc
    14281 ataactttga tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt
    14341 cggtagcttt attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata
    14401 atatcgctcc ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat
    14461 ggcgtacagc atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga
    14521 tgaacgctga atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca
    14581 atgtgaatat cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa
    14641 caggatcact tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat
    14701 ctctgatcgg actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata
    14761 ctgctgtggg attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat
    14821 ctgctatgat tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata
    14881 atttgattaa tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg
    14941 ctaagttatt atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag
    15001 caatcaagaa aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa
    15061 aagatatggt tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga
    15121 acaatatggg agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa
    15181 caccagaagg aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt
    15241 tcaaagattt agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt
    15301 taggacgtat ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag
    15361 atagtaaata cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg
    15421 atgaagatgg aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta
    15481 atattggtat ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag
    15541 atggaaatct tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata
    15601 gtaacggacg tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg
    15661 atgagtttgg taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag
    15721 tcgatgagga tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag
    15781 aactcgaaca acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg
    15841 agattacttt agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt
    15901 cagacagagt gcatcgagaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc
    15961 ttaacagcaa ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat
    16021 cgctaacaaa agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc
    16081 ctaatgttaa tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca
    16141 atcaatcaat tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat
    16201 ctacttcaga taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat
    16261 cagccgttga atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt
    16321 tagcaaaagc tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta
    16381 ttaaaatagg taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa
    16441 ctggtaaagt agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta
    16501 atcaaaaatc aactgaaaat aagtctgaag acgagtaaaa agaaggagca ttgatttgct
    16561 ccttcttttt actttgattt ctaactgaag atgtcaagga aaatttctat ttatgcaaaa
    16621 aaattatttt aaattgtct
//
LOCUS       DACCIQ010000053        16102 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCIQ010000053 DACCIQ010000000
VERSION     DACCIQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.053"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(229..1956)
                     /locus_tag="IVM62_001799"
     CDS             complement(229..1956)
                     /locus_tag="IVM62_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6845764.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1949..3142)
                     /locus_tag="IVM62_001800"
     CDS             complement(1949..3142)
                     /locus_tag="IVM62_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6845765.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3329..3973)
                     /locus_tag="IVM62_001801"
     CDS             complement(3329..3973)
                     /locus_tag="IVM62_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6845766.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4067..4201)
                     /locus_tag="IVM62_001802"
     CDS             complement(4067..4201)
                     /locus_tag="IVM62_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP6845767.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4203..6335)
                     /gene="feoB"
                     /locus_tag="IVM62_001803"
     CDS             complement(4203..6335)
                     /gene="feoB"
                     /locus_tag="IVM62_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP6845768.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6332..6805)
                     /locus_tag="IVM62_001804"
     CDS             complement(6332..6805)
                     /locus_tag="IVM62_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP6845769.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7206..7430
                     /gene="nrdH"
                     /locus_tag="IVM62_001805"
     CDS             7206..7430
                     /gene="nrdH"
                     /locus_tag="IVM62_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP6845770.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7589..9748
                     /gene="nrdE"
                     /locus_tag="IVM62_001806"
     CDS             7589..9748
                     /gene="nrdE"
                     /locus_tag="IVM62_001806"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP6845771.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9798..10763
                     /gene="nrdF"
                     /locus_tag="IVM62_001807"
     CDS             9798..10763
                     /gene="nrdF"
                     /locus_tag="IVM62_001807"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP6845772.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10852..11991)
                     /locus_tag="IVM62_001808"
     CDS             complement(10852..11991)
                     /locus_tag="IVM62_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6845773.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12300..13013)
                     /locus_tag="IVM62_001809"
     CDS             complement(12300..13013)
                     /locus_tag="IVM62_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP6845774.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13267..13968)
                     /gene="nagB"
                     /locus_tag="IVM62_001810"
     CDS             complement(13267..13968)
                     /gene="nagB"
                     /locus_tag="IVM62_001810"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP6845775.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14202..15734
                     /locus_tag="IVM62_001811"
     CDS             14202..15734
                     /locus_tag="IVM62_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6845776.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15925..>16102)
                     /locus_tag="IVM62_001812"
     CDS             complement(15925..>16102)
                     /locus_tag="IVM62_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6845777.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4983 a         3335 c         2823 g         4961 t
ORIGIN      
        1 acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc gatctctttg
       61 actcgcaccc ataaattcat aatagtttgt cggcgtcttt gaacgtttcg aagatgagct
      121 acgcattctc tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg
      181 gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca
      241 gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt
      301 tccattgttg atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc
      361 taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat
      421 ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc
      481 aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg
      541 acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt
      601 gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc
      661 tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc
      721 tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc
      781 tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc
      841 tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac
      901 atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc
      961 ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat
     1021 accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt
     1081 acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt
     1141 aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc
     1201 gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat
     1261 agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat
     1321 cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa
     1381 tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg
     1441 aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc
     1501 gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag
     1561 gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac
     1621 cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc
     1681 attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc
     1741 tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc
     1801 tggaatcact aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa
     1861 aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt
     1921 tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg
     1981 ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa
     2041 ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt
     2101 tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag
     2161 cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca
     2221 tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt
     2281 cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat
     2341 cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac
     2401 ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta
     2461 aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt
     2521 ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt
     2581 ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca
     2641 tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta
     2701 atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt
     2761 attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat
     2821 tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc
     2881 catcaatata gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg
     2941 tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa
     3001 aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct
     3061 ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat
     3121 gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc
     3181 tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa
     3241 tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa
     3301 gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa
     3361 agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg
     3421 taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa
     3481 ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg
     3541 ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg
     3601 tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct
     3661 ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca
     3721 ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt
     3781 ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat
     3841 tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt
     3901 caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt
     3961 tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct
     4021 agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct
     4081 gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac
     4141 gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca
     4201 tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga
     4261 cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct
     4321 acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct
     4381 aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg
     4441 atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa
     4501 gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat
     4561 acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat
     4621 cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact
     4681 gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa
     4741 aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct
     4801 tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga
     4861 aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt
     4921 tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga
     4981 gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt
     5041 gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca
     5101 atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg
     5161 aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc
     5221 gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt
     5281 agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc
     5341 catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca
     5401 aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa
     5461 tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc
     5521 tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta
     5581 catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc
     5641 aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc
     5701 tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc
     5761 ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga
     5821 tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg
     5881 attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga
     5941 ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac atccatcata
     6001 ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt
     6061 cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa
     6121 taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg
     6181 tcctgaatat gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg
     6241 cccggccaat tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc
     6301 ccgctgtttg gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga
     6361 ttagttcggc ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg
     6421 gatctcccag aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat
     6481 ccattaaccg tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc
     6541 ctgcttttaa ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa
     6601 agatttgacc tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg
     6661 cattgttgcc tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt
     6721 gcaaatggcg ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct
     6781 gctttgcctg cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta
     6841 aaatcattca ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc
     6901 tcacatagaa tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa
     6961 tctttagtat tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt
     7021 tttctctatt aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa
     7081 acacaaaact tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact
     7141 tttttactaa gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac
     7201 tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac
     7261 ggtttttaac agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag
     7321 cagtagactg gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag
     7381 aaacagttat cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa
     7441 agaatcaaaa cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa
     7501 acttccaaat ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc
     7561 aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta
     7621 tttcaagctg aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga
     7681 taaagaagct ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttcc
     7741 atctattatg gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat
     7801 tcaattgtat tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt
     7861 ttctttcaaa tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa
     7921 cgacggttcc gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt
     7981 tgcgaacggg gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata
     8041 tcagccagct acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc
     8101 ttgtttcttg actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc
     8161 tttgcaattg tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc
     8221 cggcgctcct atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt
     8281 attcgaagat agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt
     8341 ttatttgaac gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc
     8401 agatgaaaag attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta
     8461 tgaattgaca cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga
     8521 atatggtgtc ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa
     8581 tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt
     8641 acaacaagaa tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc
     8701 aatcgatggc aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc
     8761 ttctgtgatt aacggcaaac aggaatatga agtattagga acagatatct catgtaactt
     8821 aggctcgaca aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac
     8881 gatgactcgt gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat
     8941 ccaaaacgga aataagctga atcatacgat tggattaggt gccatgggct tacacactta
     9001 tcttgcaaaa gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta
     9061 cttcatgctg ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa
     9121 acaagttttc cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata
     9181 cgtaactgga gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt
     9241 tatcccaact gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta
     9301 tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag
     9361 tgcaagtatt catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa
     9421 aatttattac ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta
     9481 tgatatggac atgcgtaaag taatcgatgt ttatgcaacg gcacaacaac acgtcgatca
     9541 aggcatgagt ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa
     9601 gacaacaacg aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa
     9661 aggaatcaaa tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag
     9721 caatcaatgt gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta
     9781 attaaaggag cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg
     9841 aagatatcat tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac
     9901 gtatcccact ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata
     9961 cagtaggaca tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca
    10021 tggatcagtt gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc
    10081 aattcatgga atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga
    10141 aaaaagaaat cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag
    10201 ctgagcgtat caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg
    10261 tctttttaga aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa
    10321 acaacaaatt ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac
    10381 atggaaccta catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac
    10441 aagaaacgtt aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac
    10501 gttatacaga agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac
    10561 gctataatgc caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg
    10621 ccaatgatgt caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact
    10681 tcttctcaca agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg
    10741 attatttgat tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa
    10801 aagagtttag cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact
    10861 tgtttcttcc agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa
    10921 taaatagatt ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca
    10981 aatcagcatg cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt
    11041 caaaggatgg atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa
    11101 ttgttggatc actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa
    11161 accgacaatg attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt
    11221 gattccgcaa atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa
    11281 aaagctcatt acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa
    11341 tgtttcgttt aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa
    11401 gtgagacaac gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg
    11461 aacggtaaat aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc
    11521 agataaactg actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa
    11581 ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt
    11641 gcttgtttca tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa
    11701 ctgaggcact acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa
    11761 taaaaaaccg aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa
    11821 catataatag ataaaaccag caacaagcaa tggtaaaaaa attgccgaaa gcattttcaa
    11881 tattggacta aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac
    11941 tagctccact gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct
    12001 ccaaggtcct ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc
    12061 atttgcttaa aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac
    12121 aaaaacacgg tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg
    12181 gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat
    12241 ttttatttgg ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt
    12301 tattgcgcaa tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg
    12361 atcacatcgg cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact
    12421 cggtagtcat caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct
    12481 tcaaaataat cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac
    12541 tttttagtcg taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg
    12601 ataaaactga tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca
    12661 attgcccata aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc
    12721 gttcccgcgc ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct
    12781 gaacgaatcg ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg
    12841 atctcaactt ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc
    12901 atacctgctg gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata
    12961 gcgatcccta aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca
    13021 ccctccagtc caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct
    13081 cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg
    13141 ttcaatcagc tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc
    13201 ctttgtcttc tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat
    13261 tgaaaattac aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt
    13321 ttgcaataca cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc
    13381 atcagctttg ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc
    13441 gattcccatt gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt
    13501 tgcattgatt gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc
    13561 aggttcattg aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg
    13621 atgtgcatcg atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc
    13681 atctggtaca aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg
    13741 acggtagctt tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt
    13801 catttcactg aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct
    13861 tcctgttgcc aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa
    13921 tgcttttttt ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc
    13981 tttatatttt ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat
    14041 agaccaaatt gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca
    14101 taagatggtt caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg
    14161 acgctttatg tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa
    14221 acaacattac ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa
    14281 atgctggcga acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta
    14341 aaatcatcac tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc
    14401 acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc
    14461 taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa
    14521 catatagcga gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat
    14581 tagaccaagg agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg
    14641 ggatcgatgt cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa
    14701 aggtgatttt agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa
    14761 ccaatcactt agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg
    14821 gtgcgtatat cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg
    14881 cagacatcga gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac
    14941 aaaaggaaca aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg
    15001 aaaagaccga agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa
    15061 gccgacaaaa acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag
    15121 ctgtctttga ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt
    15181 tagtcatcgg atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg
    15241 aaaatattgc cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga
    15301 tcggtgagat tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt
    15361 acttttcaca agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca
    15421 gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag
    15481 ttaatcaatt agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga
    15541 aacttgcaca gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc
    15601 atatcgatca agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag
    15661 taatcatcgt ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga
    15721 ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt
    15781 gtcacaactc cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca
    15841 aaaaacggca aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa
    15901 cagcttttca caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa
    15961 aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc
    16021 taaccagcca tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat
    16081 aaagtacata aacataaata ca
//
LOCUS       DACCIQ010000054        16087 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCIQ010000054 DACCIQ010000000
VERSION     DACCIQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.054"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            226..894
                     /locus_tag="IVM62_001813"
     CDS             226..894
                     /locus_tag="IVM62_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP6845778.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IVM62_001814"
     CDS             complement(1094..1480)
                     /locus_tag="IVM62_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845779.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IVM62_001815"
     CDS             complement(1452..2834)
                     /locus_tag="IVM62_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6845780.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IVM62_001816"
     CDS             complement(2990..4177)
                     /locus_tag="IVM62_001816"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP6845781.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IVM62_001817"
     CDS             complement(4184..5215)
                     /locus_tag="IVM62_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6845782.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IVM62_001818"
     CDS             complement(5231..6685)
                     /locus_tag="IVM62_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6845783.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IVM62_001819"
     CDS             6860..7765
                     /locus_tag="IVM62_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6845784.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IVM62_001820"
     CDS             7856..8533
                     /locus_tag="IVM62_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6845785.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IVM62_001821"
     CDS             8814..9710
                     /locus_tag="IVM62_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6845786.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IVM62_001822"
     CDS             complement(9765..11135)
                     /locus_tag="IVM62_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6845787.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IVM62_001823"
     CDS             11373..12119
                     /locus_tag="IVM62_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6845788.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IVM62_001824"
     CDS             12116..12955
                     /locus_tag="IVM62_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6845789.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IVM62_001825"
     CDS             12956..13906
                     /locus_tag="IVM62_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6845790.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14043..14795
                     /locus_tag="IVM62_001826"
     CDS             14043..14795
                     /locus_tag="IVM62_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845791.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14814..15965
                     /gene="queG"
                     /locus_tag="IVM62_001827"
     CDS             14814..15965
                     /gene="queG"
                     /locus_tag="IVM62_001827"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP6845792.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5216 a         3008 c         3231 g         4632 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa ctttttttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagtgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggtgtgg
    14041 ctatgaattt tacacgtaaa gataaatata cgattaaaga agaagtcttt cgattgatca
    14101 gccagcagga aaataccgac aaggatcaag accttcaatt tcttgaagca gaattagcta
    14161 aattattcat agcaaatcaa aatgagacaa cagaaccaga tgaagagctg tacataggca
    14221 aatggttgtt tgatgaattt cagcctttag atgaaaatac cggtatcggt tattttgtgt
    14281 tcctttctcc agtcggcttc acacctggcg tattagttaa acaaaacgac gaaataaccg
    14341 tcaaagcatg gcctgcatta ttttcagtca tcaaagcaga aggagtaatc aaaaagatcc
    14401 agtcggccta ttataaaagt caatttattg ccaacagcaa attaatgaat atgttttata
    14461 atcgtctagt agaaattgct aggcctggga aaatcccgca aacattcacg tacacacagc
    14521 aaaaaaatct ctatgaagaa gtattgtatt cccgcgatct gtctgagttt tcgccatggg
    14581 aagaggtaag gctccatgaa ctgttaagta caaaaccagt ttcattgaat ttttttaatg
    14641 cattagataa caaaagaaaa ttaatttcaa caaattattg ggaagtattt gctgcaagaa
    14701 aaaaatggat ggaagaaaag aagattgccg atcagcagat ccagtttgtc cagctgaaaa
    14761 aagaaaaact atattatggg ttatacagcc gttaacagca agcgaggaag accatgacat
    14821 taaaagaaga aatcatacaa gaaagcaaac gattaggaat tgacaaaatc gggttcacca
    14881 cggcggaacc ttttgattca ttaaaagaat cgttggaaga acaaaaagca gctggccata
    14941 cttctggctt tgaacatcca aatatcgatg agcgtcttta tccagaaatg acatttgatc
    15001 agccgaaatc gatcatttcc attgcacttg cgtatcctac taggatccat gaagaagtgc
    15061 ctagggatga aaaacgcggg cagtttgctc gtgcttcttg ggggatcgat taccatgata
    15121 tactcagaga ccgtttgaac cgattgatcg aattcatcca aacaagagca gaaaaatggc
    15181 aaaaagaaga ggaatggcgt ctagctccgc aagtagatac tggagaactg atcgatgtcg
    15241 ctgtagcaca gcgagctggt ttaggattta ttggacgaaa cggattgttg atcactgaag
    15301 agttcggttc atttgtctat cttggtgaga tcgttactaa tattgtattt gagcctgata
    15361 aaccaggaga atttggttgc ggtgactgta tgcgctgcgt gactgcttgt ccgacaaaag
    15421 cattgcttgg tgacggaaga atgaatgccc agcgatgttt gtcttatcaa actcagacaa
    15481 aagggatgat gccggaagaa tatcgtaaaa aaatgcgaaa tgtcatttat ggatgtgata
    15541 tctgccagtt agtctgccct tacaatcaag gaaaagattt tcattttcat gaagagatgg
    15601 aaccaaaagt agacgaagta tatccaaaat taaagccaat gctttcgatg tctaataaag
    15661 acttcaaaca gcaatttggg catttagctg gttcatggcg tgggaagaaa cctttgcaga
    15721 gaaacgcatt gattgcttta gctaatctag gtgatcgaac ggcattgcct gagatttcag
    15781 aatgtttaac agatgtccgc cctgtcatca gaggaacagc agcttgggct attggacgtc
    15841 taggaacaaa agaaccggaa aaatggcttg atgtattaga tgaaatatta gctaaagaaa
    15901 cagaagaagt cgttataaac gaattggaac atgcccagaa attattgcgg agaaagttaa
    15961 aataaaaaaa tagttaacat tattcaaagt ttttgtccag taattgttgt aacaattact
    16021 ggtttttttg tattttttac atttttttaa gagaatttga taaaaatcaa aaattttttt
    16081 gcttttt
//
LOCUS       DACCIQ010000055        15628 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCIQ010000055 DACCIQ010000000
VERSION     DACCIQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.055"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..968
                     /locus_tag="IVM62_001828"
     CDS             <1..968
                     /locus_tag="IVM62_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6845793.1"
                     /translation="PLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLI
                     YEETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSET
                     LQRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEY
                     EIKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVF
                     SIDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIF
                     SKIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            1120..1437
                     /locus_tag="IVM62_001829"
     CDS             1120..1437
                     /locus_tag="IVM62_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6845794.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            1467..1787
                     /locus_tag="IVM62_001830"
     CDS             1467..1787
                     /locus_tag="IVM62_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6845795.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            1895..3355
                     /locus_tag="IVM62_001831"
     CDS             1895..3355
                     /locus_tag="IVM62_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6845796.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            3423..3644
                     /locus_tag="IVM62_001832"
     CDS             3423..3644
                     /locus_tag="IVM62_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845797.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            3675..3857
                     /locus_tag="IVM62_001833"
     CDS             3675..3857
                     /locus_tag="IVM62_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP6845798.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            3857..4270
                     /locus_tag="IVM62_001834"
     CDS             3857..4270
                     /locus_tag="IVM62_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP6845799.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            4393..5574
                     /locus_tag="IVM62_001835"
     CDS             4393..5574
                     /locus_tag="IVM62_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845800.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            5963..6036
                     /locus_tag="IVM62_001836"
     tRNA            5963..6036
                     /locus_tag="IVM62_001836"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5997..5999,aa:Arg,seq:cct)
     gene            complement(6105..7244)
                     /locus_tag="IVM62_001837"
     CDS             complement(6105..7244)
                     /locus_tag="IVM62_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6845801.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(7543..8178)
                     /locus_tag="IVM62_001838"
     CDS             complement(7543..8178)
                     /locus_tag="IVM62_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845802.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(8291..8926)
                     /locus_tag="IVM62_001839"
     CDS             complement(8291..8926)
                     /locus_tag="IVM62_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP6845803.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(8960..9421)
                     /locus_tag="IVM62_001840"
     CDS             complement(8960..9421)
                     /locus_tag="IVM62_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP6845804.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(9551..9982)
                     /locus_tag="IVM62_001841"
     CDS             complement(9551..9982)
                     /locus_tag="IVM62_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6845805.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(10000..10320)
                     /locus_tag="IVM62_001842"
     CDS             complement(10000..10320)
                     /locus_tag="IVM62_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845806.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10619..11395
                     /locus_tag="IVM62_001843"
     CDS             10619..11395
                     /locus_tag="IVM62_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6845807.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            11410..11613
                     /locus_tag="IVM62_001844"
     CDS             11410..11613
                     /locus_tag="IVM62_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845808.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            11629..11967
                     /locus_tag="IVM62_001845"
     CDS             11629..11967
                     /locus_tag="IVM62_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP6845809.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            11954..12133
                     /locus_tag="IVM62_001846"
     CDS             11954..12133
                     /locus_tag="IVM62_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845810.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(12176..12646)
                     /locus_tag="IVM62_001847"
     CDS             complement(12176..12646)
                     /locus_tag="IVM62_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845811.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            12733..13431
                     /locus_tag="IVM62_001848"
     CDS             12733..13431
                     /locus_tag="IVM62_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP6845812.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            13609..13950
                     /locus_tag="IVM62_001849"
     CDS             13609..13950
                     /locus_tag="IVM62_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845813.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            13943..14614
                     /locus_tag="IVM62_001850"
     CDS             13943..14614
                     /locus_tag="IVM62_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP6845814.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            14620..15306
                     /locus_tag="IVM62_001851"
     CDS             14620..15306
                     /locus_tag="IVM62_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845815.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            15309..>15628
                     /locus_tag="IVM62_001852"
     CDS             15309..>15628
                     /locus_tag="IVM62_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845816.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPI"
BASE COUNT         5204 a         2537 c         2932 g         4955 t
ORIGIN      
        1 tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact
       61 atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct
      121 tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta
      181 ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt
      241 atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt
      301 ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag
      361 ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc
      421 agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat
      481 atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt
      541 cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg
      601 agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta
      661 ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga
      721 ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg
      781 gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt
      841 atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg
      901 taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa
      961 aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa
     1021 aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc
     1081 gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg
     1141 ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca
     1201 gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat
     1261 ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct
     1321 caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac
     1381 tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc
     1441 aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg
     1501 ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta
     1561 aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac
     1621 aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag
     1681 tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt
     1741 tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt
     1801 tttgaacgga agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct
     1861 ctttatataa aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga
     1921 gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg
     1981 tgatgctttt atcggcacac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa
     2041 tgcaattatt cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc
     2101 agacaaagga atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa
     2161 cggtacacta gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg
     2221 agcttatgga gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg
     2281 tatttccttt ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc
     2341 agatgcaatc aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg
     2401 attatggggt tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat
     2461 catgggagct atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa
     2521 aatgcctgat tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac
     2581 gatcgcttta tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca
     2641 actgatcatt gatttggtgc aaaaatatat tgcagaacca ttcctaggat tatctcaagg
     2701 gttgggagca gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca
     2761 tggaccaaat gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa
     2821 tattgatatt ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc
     2881 aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt
     2941 tggcggatca ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc
     3001 agattatcta acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc
     3061 agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc
     3121 tccagtagta gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt
     3181 atcgcagcaa gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc
     3241 ggattggcgg gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc
     3301 accatttgtc atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga
     3361 acgtgaggag cgggacgctt taagtttcgt tcctcataat gaattttcta ggaggaactg
     3421 aaatgattac aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat
     3481 acaatgaaga aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg
     3541 aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac
     3601 agccttgggg aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag
     3661 ataggatggt tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg
     3721 tgttgtatag tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc
     3781 tttttctaac aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa
     3841 aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta
     3901 cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac
     3961 tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg
     4021 cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa
     4081 gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca
     4141 ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg
     4201 aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc
     4261 aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga
     4321 aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga
     4381 aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg
     4441 tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt
     4501 cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca
     4561 agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc
     4621 ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc
     4681 atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca
     4741 ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat
     4801 caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg
     4861 gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa
     4921 cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat
     4981 taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag
     5041 cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc
     5101 agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc
     5161 ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc
     5221 acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt
     5281 aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat
     5341 gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa
     5401 acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat
     5461 cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa
     5521 acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa
     5581 acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa
     5641 aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag
     5701 ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag
     5761 atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc
     5821 tccctacatt atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt
     5881 ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag
     5941 ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta
     6001 agcgataggt cgctggttca attccagtca ggaacgtata aataacccct gtatcctttg
     6061 cggtacaggg gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt atcattttta
     6121 tataaactat cgaacacagt attgattttt tctttatcct gatcttcaag ctctcgaaca
     6181 atatgaagat aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg
     6241 ctaagtatat ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc
     6301 aaagtaaatt gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg
     6361 gcgtttgatg aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggtttttg
     6421 atttcaaaat tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc
     6481 gtatgtttat caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca
     6541 tttaatttat aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct
     6601 tcccatgtta atcctagaag ttcagcaaat cgcattccag tggcaccagc aactaacacg
     6661 agcataggag aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta
     6721 aattgatcat aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt
     6781 actgcttttc tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgac
     6841 gctctaatat ggttattaaa cttaataacg gtagatttta catggtcttt agcatactta
     6901 tttaaaaagc gttgatatcc cactctatct aaatcagaaa tcaataccgc tggcatatat
     6961 ttctttatgt tcattaacgt atcttcgtat tttctataag tgataggaga tactgttcct
     7021 tctttataaa gttgcatcca atcctcaaag taatctgata ataatagatt tttgcgatcc
     7081 cctttaaggc ctttgttcaa gttatattct aattcgttgg cggcatcttt tgcttctcct
     7141 tttgtcttaa atcctgattt tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa
     7201 atttcgtatt gccaactatt tccacgtttg actaatcttg ccataattga atcagctctc
     7261 tttctctgat acaataggaa ctataaagaa gcccattgtt tgggttttat tattttttgc
     7321 acatcctttt tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg gtataattgg
     7381 atgtgtatta gatcatccaa tcagaggtgt ttattatgat taaagtgatt tttaaaagaa
     7441 taactgaact tccagagttt agattatttg ttgattattg gtatgtctgg ttttttattg
     7501 ttgcattgct gataggtcta gatgtcatat tagaaagaaa acttaagtaa gagttgagtt
     7561 aataaattga attcctaaat gagatatttt gtagcccata ttttgtttgc cgacatcacc
     7621 taatactatg gagctaatcg ttttttctaa cagaccaaaa tcagataatc tatttaagct
     7681 accatctatt agctcttgtg aatagccaga catgctgata tttccagtga aattttcgtt
     7741 tccacgactc attaagcatg ctaacaattg aatctcaatc gatgttaatt gattcagtac
     7801 aagaagataa tactcctcgt tatcccatga agcgctgtta attcgaagta aacaattctt
     7861 ataaagattt ttatacatat ctattttatt ttgtgccttt gatttttcta cctcgtcatt
     7921 tatcccttcg aatattccaa tgagttggtc cttgttctct aagttgaaaa catcttctgg
     7981 aatttgatcg cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg
     8041 tttgaaacgc ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc caccaatata
     8101 aggaattgct ccaataccgc cttcaactat taattcaaga tattcttttt tggttagttt
     8161 ttcattgcta ttatccatag ttacctccta ttttgtcatt tcctttacaa gcattgtgac
     8221 tcctgatggc actcttttgt ataggtttta ttattgtgca caatctaact cttagtgggg
     8281 cgtggttcat ttatttttta cttactttac tattaatcat atcccattga gcataggatt
     8341 tttctatgtt cgatcctctt gaactaattt ctcctgtaaa agttacttta tcgccctttg
     8401 taaaggtatc agcattgttt aagtccttcg caaaaattac atatgaaact ttaggtgttg
     8461 cagaaatatc attccaactt ttatcatcgt actctttgtc tgctataatg gctattctag
     8521 tagtcattga ttcgataaca gttccttcaa aagtatatgt tttaccacta atatatttag
     8581 taaaagctgt attttgctca gtagctggag taatagaata ataactatct aagaaagcac
     8641 cccagtcatg attatttgtc tctaaaaatt cttcaaattg agttaggctt ttattgctgc
     8701 tgttagatgt agtagtttga gtatcagatg atgagctttc ctgtttgtta ttgtcatttg
     8761 cctcgtttga tgaggaaagc gagaaaccta cggtagtaaa aataatcccg ataataagga
     8821 gaatcatagg tgttttctta tttgtttttt tgataaatct tacaattaac atgattaaac
     8881 ctataataaa taaaacaata ccagtaaaac caaatatacc gtccattaat agatcctctt
     8941 tctcttttga tattgaatat tataaaccaa gcaattcttt tttcttaaaa tcgaattctt
     9001 cttgattaat aatcccttca tctaacaact ctttatactt tttaatttca tctgcacttg
     9061 aaatttgtgt ttctttggaa ttattatcta atatagattg ttgcttctct atatattttt
     9121 ttaaattttc catttcattc caatattttt ttgtaaacat aacggtgttt tcatctttcg
     9181 tggccgctaa tatacctccg cgactttcgt ttccgcctag caaggtaaat tgtatataac
     9241 cattactcat acctggtttt tttaattgta cggctgatat atttttaaag gggatagttt
     9301 tttctccttt aataccttga ttaactaaat ttataaatcc ttttctaata attgaaatag
     9361 aatcatcagt tactttaatc aaggtttttc ctggagattt tattaaaatc tctttttcca
     9421 ttttttcttc ctcatttctg tgatatgatt ttttttgtag aagctcatat atgaggaaag
     9481 agcccgtgtt gcagcacggg cttttttaat gttttgctga aatcggtttt tttaataatt
     9541 cttgatgtat tcatacatat tgccttgcgt aagaatattt tttcttaaaa tagcattggc
     9601 agacagcata acaagtgtat ctgagcttat tctgaataga ataatactcc ataaattttt
     9661 cgagattaaa ctgagattca tcagtcagtt cgttctcaat atagatattt aataaaatta
     9721 gaatagctat tttatctgct tctgtttcaa attttgagtg aaaagttgta gaagtatcgt
     9781 acaaaactga aaattcaaaa tgagaggcgc tgaaatgtgc aagttcatga gataaatgaa
     9841 aggcttctgc agtttcaccg tatagatttt cattcaaaaa aatgattctg ggttttggat
     9901 agtagaaacc aggctctttc atctccatat aaactacttt caaattgtat tcgctcaaca
     9961 tttctttcaa tttcaaatac atacaaacca tcactccaac tattcatttt cctctaaagc
    10021 tttagcaatt gcaatcgctt tacgcattgt ctccttagat atttcttttc catcaaaaga
    10081 aaaaacagta tcgtcttctg ataaatccac atgtttaggg gtttctcttt cttctctacc
    10141 tagaaggtag tctacagaga catcgaaata gtcagcaatc cgacttaatt catctgaatt
    10201 aggagtatta tttttccact ttgctagata accgttagaa tatccaaaat taatttctaa
    10261 ctgccgtata gatatctttt tcctcttcgc caagtctttt attatttcat aggtattcat
    10321 cgataaaaca acctttctaa atgtttacaa aaaaagttta gaaaaatacg cagaaataat
    10381 ttgactaatt ctgagtaatg agctatacta tatcttgtaa acagatttaa caactaaaaa
    10441 gacaacaaaa aacactattg atatgtaaat gcagaccgcc aagaaagctt taaaatcaat
    10501 gtttttatgt cttatttaat tatgctctta gtatagagta ttactcagat actgtcaatt
    10561 gaatttagaa aaaagttgtt aaatttgttt acgaatataa aagaaaggaa tgagaaatat
    10621 gaacacaccg caaattttta atttcgaaca aaacgaagtt cggacaattt tagtaaatga
    10681 tgaaccatat tttgtaggca aagacgttgc gagtgttttg ggttattcaa acactaaaga
    10741 tgctttgtcg cggcatgtag atttggaaga taagatgggg tcgcgaatca cgacctcagg
    10801 tcaatcaaga gagatgacaa tcatcaacga atctggtttg tacagtttaa tcttaaaatc
    10861 aaaacttccc tctgccaaaa aatttaaacg ttgggtaaca agtgaagtgt tgccagcaat
    10921 tagaaaacat ggaggttatc taactccaga aaaagtagaa gaagctttgc ttaatccaga
    10981 tacaatcatt caattagcaa ctcaactaaa agaagaaaga actggaagat taatcgcaga
    11041 acaaaagatt gcagagtacg aacctaaaat ctcctattta gatagcatat tatcttctac
    11101 agattcagta acaattagtc agattgcagc agattatggg atgtctccac aacagatgaa
    11161 taaattactt cataaactag gtattcagaa aaaagtcggt aaccaatggt tattgtgcaa
    11221 aaaacacatg aaccaaggat acacaaaatc tcatacaact gagatcccga aagccgatgg
    11281 tggcactaaa attgtaatga ataccaaatg gacacagaaa gggcgtctat ttatctacga
    11341 attactaaaa aaagaaggat attaccctca aatggattta gaggaaattg gttagaaagg
    11401 agttttagta tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa
    11461 tatgtcgaaa gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc
    11521 aaaagctact tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt
    11581 atagattcga ttatcactat gtacgaattg taaagggagg aacagctaat gcaatatcta
    11641 gaagcaaaaa tcccaattcc agaaggctat gtaattatct cccaagtgga ttatgaggag
    11701 ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc cagaagtgct agaacggatt
    11761 aacagaaaat atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt
    11821 atcgatatcg agaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca
    11881 tatctatttt taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta
    11941 aggaggcaag cggatgggaa aatttaatag agcgctagtg ttcagcgcac cgctaatcat
    12001 ctacgcttta ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt
    12061 ttggattttt atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga
    12121 gggggctgac taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt
    12181 ttaattcttt cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt
    12241 ggatttgggt tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa
    12301 aattaatttt ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat
    12361 ttagcggtac agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta
    12421 atttcttaaa tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat
    12481 taactaacat agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat
    12541 ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt
    12601 ttatccctgt taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg
    12661 ttaagtgaaa aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg
    12721 agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc
    12781 ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca
    12841 ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca
    12901 tatggcagaa atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg
    12961 gggtttacag gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa
    13021 atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat
    13081 caattagaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga
    13141 attagcggac aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt
    13201 ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt
    13261 tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga
    13321 ttcgatgatg ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa
    13381 attgatcaac tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc
    13441 gctaagaata gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct
    13501 aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg
    13561 gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa
    13621 ctttccactc tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa
    13681 acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa
    13741 gatgatttag gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag
    13801 cattcgaata aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa
    13861 gagtacgaca gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt
    13921 gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact
    13981 ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg
    14041 ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga
    14101 gtacaaacac tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa
    14161 accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca
    14221 cacggaaacc gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa
    14281 tgcaatgcaa atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg
    14341 actgggatta tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa
    14401 gaaattaaac atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat
    14461 gaccaaaacc ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga
    14521 taacgttgaa actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat
    14581 agcagctaag agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat
    14641 tcatattcag cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag
    14701 gaagtgaaca ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt
    14761 gaaattcgtt ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg
    14821 ctgataggcg atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc
    14881 tactttcgtt ttgaaggtgt cacagggcgc aaaataagcc tgaaagacga atcgaataca
    14941 actgtgagtg atgccaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat
    15001 attcctttca aagaaggcta tgagatttta cctgcgaatc aagaatatta cttctacaaa
    15061 tgcatcacaa aaagagtctg ctgcatctgt ggcaaaacag gagctgacat cgatcacttt
    15121 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc atgcagagta cacatttgca
    15181 gcactctgca gaatccatca cacagagaag cacaaaatag gtgtgattaa tttcaaaaat
    15241 aaatatcaaa tcaaagggat caaattaaac cagaaaacaa tcaaaaagtt aaatattgga
    15301 gggtaaaaat gacagaacat cgaagttatt atgcgattat accagccaac gtaaggtacg
    15361 acaaaagact taaaccaaat actaagttgt tatacggaga gataacggcc ttgtgtaatg
    15421 aaagaggctt ttgttgggca ggcaatgagt actttgcaga tttatatggt gtgaataaag
    15481 agaccatatc gcgatgggta agtgatttga ttaagtttgg atacttgaat cgggaaatca
    15541 tttacaaaga gggtaccaat caaataatca ataggtacct acgaattaat caatacccta
    15601 ttgacgaaaa acgcaatacc cctattga
//
LOCUS       DACCIQ010000056        15563 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCIQ010000056 DACCIQ010000000
VERSION     DACCIQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.056"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..212
                     /locus_tag="IVM62_001853"
     CDS             <1..212
                     /locus_tag="IVM62_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6845817.1"
                     /translation="TEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTL
                     LGVVGMVFASFAIWLFIKKRTGVKK"
     gene            209..2086
                     /locus_tag="IVM62_001854"
     CDS             209..2086
                     /locus_tag="IVM62_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6845818.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            2178..3020
                     /locus_tag="IVM62_001855"
     CDS             2178..3020
                     /locus_tag="IVM62_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6845819.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(3172..4047)
                     /locus_tag="IVM62_001856"
     CDS             complement(3172..4047)
                     /locus_tag="IVM62_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6845820.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            4254..5285
                     /locus_tag="IVM62_001857"
     CDS             4254..5285
                     /locus_tag="IVM62_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6845821.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            5374..5679
                     /locus_tag="IVM62_001858"
     CDS             5374..5679
                     /locus_tag="IVM62_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP6845822.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            5858..7873
                     /locus_tag="IVM62_001859"
     CDS             5858..7873
                     /locus_tag="IVM62_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6845823.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            7875..8159
                     /locus_tag="IVM62_001860"
     CDS             7875..8159
                     /locus_tag="IVM62_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6845824.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            8185..9558
                     /locus_tag="IVM62_001861"
     CDS             8185..9558
                     /locus_tag="IVM62_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6845825.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(9641..11656)
                     /locus_tag="IVM62_001862"
     CDS             complement(9641..11656)
                     /locus_tag="IVM62_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP6845826.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(11678..12892)
                     /locus_tag="IVM62_001863"
     CDS             complement(11678..12892)
                     /locus_tag="IVM62_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6845827.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            13113..13915
                     /locus_tag="IVM62_001864"
                     /pseudo
     CDS             13113..13915
                     /locus_tag="IVM62_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            14211..15155
                     /locus_tag="IVM62_001865"
     CDS             14211..15155
                     /locus_tag="IVM62_001865"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6845828.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         5188 a         2837 c         2931 g         4607 t
ORIGIN      
        1 aaacagaaat tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta
       61 tattgaaagt agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa
      121 cgaaaaatac acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt
      181 ttatcaaaaa aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt
      241 aggagtcctt ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt
      301 tgcagaggag acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga
      361 tcctttaatc caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc
      421 cgatatttca ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg
      481 agcgtccgtg gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc
      541 ttcaggaacg acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg
      601 gaaagatgca gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa
      661 catggtttta gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata
      721 cgggacagaa gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg
      781 aacgttgaaa gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt
      841 tattatttct aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga
      901 tggattgtac acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc
      961 ttatgacatc ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt
     1021 taatcattta gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga
     1081 aatgattgaa aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt
     1141 agaaaagacc atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg
     1201 aaaagatgtc gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg
     1261 tatcgctgat aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca
     1321 tgacgctgct ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga
     1381 tggaaataaa gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac
     1441 ggtttcagtt gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt
     1501 ttactatatg catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa
     1561 tgtcgataac gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg
     1621 caaacgattt gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga
     1681 attcgtcgtt cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac
     1741 aaaagccgtc agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa
     1801 cggtttaatc gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc
     1861 gccagaaaaa tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta
     1921 tgtaacagca ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact
     1981 tccttcaaca ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact
     2041 gattgctgga ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa
     2101 ttggaaaaag gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca
     2161 tcacaaggag aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt
     2221 tcttgcttct ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata
     2281 attatctgga tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag
     2341 aagtcatggc taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag
     2401 gtggaaatcc aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa
     2461 cctattttga gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc
     2521 caatttttga tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa
     2581 cgtcttatcc tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc
     2641 ctcaagcaaa gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg
     2701 aaatcaatgg acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg
     2761 atacaaaaga tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac
     2821 cttatatggt caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa
     2881 aagaagttca aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat
     2941 tgttagctgc atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca
     3001 aaataagaaa cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc
     3061 gtaaacaaac aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca
     3121 aagagagcct atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt
     3181 ccgatattct ttaagaaggc ccttttgcat caattcttgt ttgtttttca agttgcggat
     3241 attctcttca aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa
     3301 ggcaatttga gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac
     3361 aagacacata tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt
     3421 aggggcctct acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct
     3481 gtagctaata aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc
     3541 actgtattct tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt
     3601 ccttgccgtg atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa
     3661 tactttaccg atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga
     3721 ctggatcgat aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa
     3781 aggaaaatta ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc
     3841 cagctcgaaa cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat
     3901 agctgaatgg gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc
     3961 ttcgatattc ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg
     4021 ttttgttagt ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa
     4081 cacttggttc cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca
     4141 aaccgaatga taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact
     4201 ttctgcgctt ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta
     4261 caatcgctta tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa
     4321 aacttacgaa caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa
     4381 cattcgaaga catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc
     4441 aattagttgt catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg
     4501 aacatggtaa gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag
     4561 agttatttgc attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt
     4621 tcgattctga cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt
     4681 tagaagtaga aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat
     4741 tttctactgc gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt
     4801 cttatttcgg cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac
     4861 gaatgaatga ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat
     4921 ccagttattt ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aaagggatgt
     4981 tcgtgaaaca aacaaaagat cgacaagaag aacatctgaa aatgttttac atgcctgatc
     5041 atgaagactt tggtatcgat ttgcccgaac attatggtgt attgacctat atagatgaac
     5101 aagggagtta tcatgaagaa aaagtaattt ccgaggttgg tgattacagc cgtgtctacg
     5161 aagggattta cgagacatta gttaatggcg cagaaaaagt catcaaagat gaagaaacaa
     5221 tcttacaaat gaaaattttg gaagaaggaa tcaaaagcat tcaagaatca gaaggaaaaa
     5281 tatgacatat ttgtaagaat cgggtttgtt ttttgacgca taaactgtct tattgtacga
     5341 tgaaaaagta tagaactact aggaggaacc aacatgacaa attatgacgg tgaaaaacac
     5401 aaacgattaa gaggttcaat ggcccctaaa acagaagaag aagtggcttt agcaggaact
     5461 aattctggtg aaacagaaga caaagaagaa tcattagctg gcacaaactc tggtgaaaca
     5521 gaaaataaag aaacagcttt atctggttca aattctgtag aagaggaaac aaagcctaat
     5581 gagcccaatc actttgaaaa agatatcgaa aaagcggttg aagatgtcaa agaaaaattt
     5641 gaagaactga cctcaaaaaa taaacatgat catcattgat cggacgaaat caattcataa
     5701 aagcaggaga catttggtca cctgcttttt ttctgtatcc attcgataga aatcgttcgg
     5761 tacagtcgta aaaaaaatcc aactgtaagt tgtaccaaac acccctcgat ttaaaagcgc
     5821 taccatacta gaatataagt aggaggtgaa cagcgctgtg aactacaaaa ttagttatct
     5881 cctgaaaaca ccaagagaaa tcagtctaga aaaagcagca gagatcatcg aagtaaatac
     5941 aagcgaggcg atcaaacaat taactccttt tgccgaatat gtggatgata cccgtatccg
     6001 tataaagaaa ttccgagcaa tcgactgggc agaaaaattt ttggatatta ctcgatttga
     6061 agctgtgtat accgaacaag agcgaagaga tcttctttat ttgatggttt attcgactgc
     6121 aacgtctgtt tctgtttttc attttcaaga ttttcttcag gtttctaaag ggacagtcct
     6181 gtcagatatc aaaaagataa gaaatgaatt agaagtggaa gattgtcagc tgtcgtatac
     6241 taggaaaact ggttttaggc tgctagggtc agaagcaata ttaagaagag aagcaaaaaa
     6301 tcgcactgct cgtttgatgc agtcacttgc tggaagattt gggctcacgt atttaaccgc
     6361 aaccctttcc attgattatt acgccaaagt tagagatttg ctagataaaa cgatttcact
     6421 atcagatctt cgattcgcac caaatcgaat ggatgaatta gcttattatt tgagtttgac
     6481 aaccattcga attggaaaaa catccatgga actggtaaaa gatgctgaac tgttggcttc
     6541 tcttgcagtc actgacatta gtcgacaagt attgactggc cttggctatt ccgaacatca
     6601 aaattcggag attatatttt tctctctttg cttagcatcg atcgttgaag gagatgtcca
     6661 ggagcctgca cttgattttt tgctggaatg ttcttcagaa gtcatccatc gcatggaatt
     6721 cctgatggcc gtagaatttg cttcgtttcg tgaattattg atgaatgtct tttatcactt
     6781 ggtccctgcc tattttcgta tttcctattc tttttattta cctaatgtga tgatcgatca
     6841 aattaagcat caatatgctt ccatctacga aatgacaaga aaagcattac ttccgttaga
     6901 aagacgaata gggaagagca tccctgaaga agagattggt tttttcacta tactatttgg
     6961 cggagagatc agaaaagcgg acgcagaaga aagaaataga aaaataagag ccgtgatcgt
     7021 ctgtccaagt ggcattagct cttcattgat cttaaaaagt gaacttcagc aattattccc
     7081 gatgatcttt tttacagaga ctaattcagc tgaccgctta cacgaggttg acgaaacttc
     7141 ttacgatatt attttttcta ctgtacctct cacacttact gataagaaaa agtctctcta
     7201 tgttctgcat ccaatcatga ctgacttgga aaaaaatcag ctgatcaatc aagtacaaag
     7261 agattggctg ataccaggat tttcaatgcc ttctgcccaa gaattgttgt cggcgctact
     7321 gccttatatc gaattaaaag aaggcgtaga cgaagaaaag ctctatcaag tattaaatcg
     7381 taaaatgaac aaacttttag aaaagaagga ggatcaacgt cctatgctat ctgaattatt
     7441 aacagaagaa atgatccaat tatcggatca accaaaaaac tggcaagaag caatcacatt
     7501 tgccgcacag ccgctattat cagaaaataa aatagaagaa agttatatca aagctatgat
     7561 cgagcgcgtg gagcagtatg gtgcgtttat ccatattggc gatcacatcg cattacctca
     7621 tgctcgtcca gaagatggcg taaacgaagt agggatgtct ttgctgaaac ttcaacaacc
     7681 tgtggatctg cttgataatc cagcccatcc cattagtttg ttcatctgtt tagctgctat
     7741 cgataatgag gcacatcttc gggctttggc aaatttaaca aagctacttt ctaataaaca
     7801 aaatcttcaa gatttattac acgcatcaac aaaagaagaa atattagcga tcataagaaa
     7861 aggagacgaa tgatatgaaa atttttgcag tatgtcaaag tggattagga acaagtttta
     7921 tggtagagat gaacatcaaa caagcacttg cagatcttgg agcagatgtg gacgagtttg
     7981 aagtaagtca tacggatgtt ggaagtgtat ctagcgatat ggctgattat ttcttcattg
     8041 agcattcttt gaaagatacg ctaacttcta tacctgatga aaagctcgta cctttgcaat
     8101 cgatcattga cagcgaagaa gtaaaagaaa aagtaacgaa gatcttgtca aaagaataaa
     8161 aatgattcaa gggaggaact atcaatggat aaattgttga atttgtttgt gtcaatcgtc
     8221 agtcagccct ccatcctcgt ttctttgatt gcactgcttg gtctcgttct tcagaagaag
     8281 aaattttcag aagtgatcca aggaactatc aaaacatttg tcggattcct agtactgatc
     8341 ggcggtgctg gtttaatcag taattcacta accccttttg cttcgatgtt ccaaacagct
     8401 ctgcatactc aaggagtagt tcctagcaat gaagcagtag tggcgattgc tttgcaacaa
     8461 tacggtacac ctactgcttt gatcatgcta gtagggatga tcgtgaatat tttacttgct
     8521 agattcacaa gatataaatt catttttctt acaggtcaag caatgcttta tgtttcttgt
     8581 ctaacagcag tcattttagt aagcgcgggg tttactgcaa atttgtggat gatcttatta
     8641 ggcggggtct tcgaaggaac actattaact attacgcctg ctctttgtca accgtttatg
     8701 aagaacatca ctggaaacaa tcaagttgcg ttagggcata ccggaaatat cggttatgct
     8761 tctagtggat ggatcggaaa gttttttgga aacaaagacc gttctacaga ggacataaag
     8821 attcctaaaa gtttcagctt tttgcgagat tctactgtat cgatcatgct cttgatgtcg
     8881 atcgtatatg tcgcacttgc tttgttagct ggtcctggat ttgttgaaag agaactaagc
     8941 aatggagaca acgctgtaat ctttgcactg atccaagctg gaacattcac agctgggttt
     9001 gttatcgtcc ttcaaggggt tcgaatggtg ttggcagaaa tcgttccagc attccaaggg
     9061 attgctaaaa aactcgtacc cgattctaaa ccagcattag atgttcctat cgtttttcca
     9121 tatgcaccta atgctgtatt aattggcttt tttgttagct tctttgtagg aacactatcc
     9181 atgtttgcta tgataggctt gcaaactaca gttatcattc ctggggtagt aggacatttc
     9241 ttctgcggag ctgcttctgg tgtcttcggc aactcgactg gcggtagaag aggggcggta
     9301 ttaggttctg ctttcaatag cttattgatt agttggctgc cattatttat ccttccagta
     9361 ttaggagatc ttaaaatcgc ttcttctaca tttgccgata ctgattatct agtaccagga
     9421 cttgtacttg ggaaactagg agaatacggt caaatagcct tgacagttgg aatcatcgct
     9481 ttctttttac ttgtcgtatt atctagcttt ctttttgccg gaaaaagaac aaacattgtt
     9541 tctaaagaac agaattaagc aaacttcaaa ataaaaattg cttggacaat atcagaagaa
     9601 gatttaaagt cttctgatat gtcgcaagca gttttttgtt ttatgcattt atttttgaat
     9661 agcttgttaa gacaagtggc tgttttgcta gaagaggata acttttacca ttcattttca
     9721 ctgttccgcc tgttctatca gaagtaatca cgatcttatc ttttgttttt tcaagcgaaa
     9781 tctttccatc tttgataggt agcgtacaat tcagtgtttc aaatgaatct agattaggtt
     9841 gtatcataaa ttcttcataa cctggctttg ttggatacaa accgataaaa tagcgtccta
     9901 aaagatagat tgggcttgct ccccatgcat ggcataagct ttttccataa ggatctccat
     9961 acatactgta cttttcattt cctgtttgag tagggtcgta ttcttcccag aaagttaccg
    10021 catcatgaga taacatccct ccccaataat caagaatcgt ttggtagacg atattcgtct
    10081 gtccgagttt gcataaagca tcttgttcat agaatttgaa atatggagta gtaatctgtg
    10141 tgatagtgtc attcaataag acattcttta agatcgattg ctgttggtct ttgttagcaa
    10201 aatcgaataa aacagcgaaa atattcgcat gacgcgtcac atttcttctc ccagattcat
    10261 agcaatcgat aaaagcgcca agttcttcat cccaaaagta gtcaaatagg ttttgtttca
    10321 atagttgata tttttcttga tagagatgag tatgataatt ttcgttctct ccatatgttt
    10381 cagctaagat atctccgcaa gtgatcatgg aatgataggc tttcagtaat aagacttgtt
    10441 ctgcagcaag cgttccttct ttatccattt cagaccaatc aataaatatc caatcttttg
    10501 ctctcccata aataaaccct aattcattcg tttgctccat caagtatctc attaatgatt
    10561 gcatcttagg gtagacgatg cgtaaaaatt catagtcttt cgtcatcata tagtggtttt
    10621 cgataccaat caaccacaag atcgaatagt caacgatagt attcatatgc tgcttgattt
    10681 cattttgtcc tcttaatgct aatatcgttc gtttattgat ttcctcgtca aaaaaaagat
    10741 attgattgat catgaatgat tgatacgcat ctccagacca aatccagcga tctcgtttga
    10801 taccgtcgat aaaaaacaaa tcactacata acttgaaggc ttcttctgat actgaccaga
    10861 tctgattcag taaagaagaa tcgcttgaaa aagcagcttg tttcgaaaga gataaaaatt
    10921 catgaaaaac ttgaagttga accattccag tagaaacctc tggtataaac aaatagcgaa
    10981 aagcgcgttt acgagctttc atttttcttg tcattcctat ttgcttatag tagcaataat
    11041 cgatatccat agcttctaca tcggactctc cataacatag agtgatagat tcgatatggt
    11101 catccagaga tattagttct acacagccat tcactgctcg cccaaaatcg aataagattc
    11161 ctccgccaat ctccgtttca gtgactggat ggacgcattc tttttgataa tagatttgat
    11221 ttggatcttt tgcgatatcg acataaagcg gactccagcc agctggatat tcttcaataa
    11281 aattacttgc tgtccagcca atatctgatt ttacttcgtc tccctcgata aacatcgctg
    11341 gtagccctga tgtatgtcct acaaagatac gaatttttgc tttaccagct ggacaagtca
    11401 gccattttcc acatggatgt ttttgtccat taatttctac atacccaact ccttggatcg
    11461 tcactttaaa catggaaggt tgatcaagaa agtaatatct cttaaacttg acgttccgat
    11521 ggcaatcatc catgtaccag tacgctggcc agtcaaaacc tcgttcttcc cgttggaaat
    11581 tttgtagcag tccatggcgg atctcaaagt ctccaggata ccaaagccat ctatctttat
    11641 gtttttcatt tgtcatttta cacgacctcc atcaaattta ttttgttgtc aattgttctt
    11701 ttatttcttc atctactctt atcactgtct ttctattcgt cctgtgtact actagaaggc
    11761 taataagcgc gctaatcaac agcaagatac tgccattgac gaaaacagca agtgttttac
    11821 tcccaagaga agcaaaagag agaaagagca aaccatatgg tgcaatgaaa gtacctaaat
    11881 tagcgccaac caataagatt gaagtattga aactctccgc gtacttgggc gcttggatac
    11941 ttacttggtt gaataagtaa ggaatgaata acgaaaaaga aattcctgtt aggatcgcac
    12001 caaacccaat cacatacagg tttatcacag tggcaatcaa tacaaagccg attcccatca
    12061 gtagtaaaga aagtgtaagc atatagcgat gtgcttttgt ctgaagccag ccaaaacaaa
    12121 ctccggcagc catacttgcc aattgcatga ttgttaagat attcgctgca ttagtttcgt
    12181 ttcctagaga ataagtcgtc aggactgtgg ctaatttaat cgatacagca ttatatacca
    12241 cgatgaccag caagagaaaa ataccataac taatcatttt aggattcgtt ttgattactt
    12301 gtcttgttgg ggctgtgcca tgcgattgaa gttcagttgt ttcaggtgta ggaacaaaca
    12361 aaacaaacaa gacaagaata ggtaagctga tggcgtagac taaaaaggta tattgccaac
    12421 cgaactgtac tagctgtcct gcaacaaatg tcattaaagc tgcaccaaat ccttgaaatg
    12481 cactttgaaa accaatcata gaggctcgtt catctccgtg ataaaaggca gcaatcatgc
    12541 tgactgccaa agaattgatc aatccaaagc caatacctag tccagctctt gaagctaaga
    12601 caatactgta actttgtgag aagaggggga ggataccaca aatcgatgca atcactaatc
    12661 cgatgatcac agtttgtttt tgtccgattt tcctagcaat aaaactacta atcaaaacaa
    12721 aaatagtgac cataaaagag ggagcagtcg ttaataattc aacagcagat aaagaacgcg
    12781 tggcataagt agctgacata ctgggaattg cagacgtgac tgcaccagca gaagtcaaaa
    12841 taagagagac tgacagcaga gaaaatttca tcatcttttt attcataagc atgttccttt
    12901 catttttgta agcgcttatc aaaaatatag tatgaagtgg ttattctctc aatagcggaa
    12961 tcacaggaag ttcatgtccc aaccatagaa agtctttatg aaaccaaaaa tagctttgca
    13021 tataatagca gtacagcgtg aactaataag cgaaagaaac cgcttacaaa aaggagggag
    13081 tcaattggaa ctagctgaat tagacatttt ttttgcgtaa cgaagaatca atcgaaactc
    13141 tgcagcgtga acaaggaatg aatatcaatg atttaaactt agcttaccat cttcagacac
    13201 cgccaaaaat ccaccgaggg gatctatttc aacagggaaa tattcatatc agtaaacacc
    13261 tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat taaaagaaga
    13321 aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac tttcttcttt
    13381 tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt ttcgcggcgg
    13441 aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt ttgatacata
    13501 cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg aattgactca
    13561 tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg agatgcttag
    13621 agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa cagcagctca
    13681 ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg atgcttcctt
    13741 tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc aagaaacgag
    13801 tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt ctttattcaa
    13861 attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac attaagaaga
    13921 aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct gtcttattat
    13981 atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt tgtattatca
    14041 ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga attcgtttcc
    14101 atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa taaacaagac
    14161 tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc atgaaaaaaa
    14221 caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc gcttatgcaa
    14281 tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa gaaaaagcag
    14341 aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat gtggctgtat
    14401 ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca gctggtatca
    14461 accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca atcatgcgcc
    14521 aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt gcttcaaacc
    14581 cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca tcacgtgtta
    14641 tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca ttgaaattaa
    14701 aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat tcagaagttg
    14761 ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta gaaaaagacc
    14821 atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat gctgcttatg
    14881 agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact gcacgtatcg
    14941 taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat ttaactggtg
    15001 aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa aatggtgttc
    15061 gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc aaatcaacaa
    15121 gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg aactaaaaat
    15181 cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat atctaatagg
    15241 taagcaaata aacagaatgg ttaaaattaa tcggtccgaa aatgtatccg tacatatgaa
    15301 catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt tatctgtggt
    15361 taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt cgttcatatt
    15421 tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa aaaagtaaga
    15481 aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa aatgacgatt
    15541 tttgataaaa aaccgttcga gag
//
LOCUS       DACCIQ010000057        15162 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCIQ010000057 DACCIQ010000000
VERSION     DACCIQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.057"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVM62_001866"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVM62_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6845829.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVM62_001867"
     CDS             complement(322..891)
                     /locus_tag="IVM62_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP6845830.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVM62_001868"
     CDS             1077..1979
                     /locus_tag="IVM62_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6845831.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVM62_001869"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVM62_001869"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP6845832.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVM62_001870"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVM62_001870"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP6845833.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVM62_001871"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVM62_001871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP6845834.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVM62_001872"
     CDS             8547..8732
                     /locus_tag="IVM62_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP6845835.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVM62_001873"
     CDS             complement(8794..9990)
                     /locus_tag="IVM62_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6845836.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVM62_001874"
     CDS             complement(10160..11596)
                     /locus_tag="IVM62_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6845837.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVM62_001875"
     CDS             11889..12698
                     /locus_tag="IVM62_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6845838.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IVM62_001876"
     CDS             complement(12884..15037)
                     /locus_tag="IVM62_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP6845839.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4436 a         3209 c         2676 g         4841 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttttgt tt
//
LOCUS       DACCIQ010000058        15145 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCIQ010000058 DACCIQ010000000
VERSION     DACCIQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.058"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(22..252)
                     /locus_tag="IVM62_001877"
     CDS             complement(22..252)
                     /locus_tag="IVM62_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845840.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IVM62_001878"
     CDS             complement(688..1254)
                     /locus_tag="IVM62_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6845841.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IVM62_001879"
     CDS             complement(1254..2210)
                     /locus_tag="IVM62_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP6845842.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IVM62_001880"
     CDS             complement(2210..2866)
                     /locus_tag="IVM62_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6845843.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IVM62_001881"
     CDS             3236..5254
                     /locus_tag="IVM62_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6845844.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IVM62_001882"
     CDS             5681..8095
                     /locus_tag="IVM62_001882"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP6845845.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IVM62_001883"
     CDS             8443..9996
                     /locus_tag="IVM62_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6845846.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IVM62_001884"
     CDS             complement(10377..12161)
                     /locus_tag="IVM62_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845847.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IVM62_001885"
     CDS             complement(12176..13897)
                     /locus_tag="IVM62_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845848.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IVM62_001886"
     CDS             13997..14758
                     /locus_tag="IVM62_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6845849.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4924 a         2776 c         2983 g         4462 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggca
//
LOCUS       DACCIQ010000059        14621 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCIQ010000059 DACCIQ010000000
VERSION     DACCIQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.061"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            120..1859
                     /gene="yfcC"
                     /locus_tag="IVM62_001887"
     CDS             120..1859
                     /gene="yfcC"
                     /locus_tag="IVM62_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6845850.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2016..3200
                     /gene="iadA"
                     /locus_tag="IVM62_001888"
     CDS             2016..3200
                     /gene="iadA"
                     /locus_tag="IVM62_001888"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP6845851.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3311..3514
                     /locus_tag="IVM62_001889"
     CDS             3311..3514
                     /locus_tag="IVM62_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6845852.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3615..4277)
                     /locus_tag="IVM62_001890"
     CDS             complement(3615..4277)
                     /locus_tag="IVM62_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845853.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4290..5792)
                     /locus_tag="IVM62_001891"
     CDS             complement(4290..5792)
                     /locus_tag="IVM62_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6845854.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6340..8019
                     /locus_tag="IVM62_001892"
     CDS             6340..8019
                     /locus_tag="IVM62_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6845855.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8146..8625
                     /locus_tag="IVM62_001893"
     CDS             8146..8625
                     /locus_tag="IVM62_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP6845856.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8844..9524
                     /locus_tag="IVM62_001894"
     CDS             8844..9524
                     /locus_tag="IVM62_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP6845857.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9532..10863
                     /locus_tag="IVM62_001895"
     CDS             9532..10863
                     /locus_tag="IVM62_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6845858.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10878..11840
                     /locus_tag="IVM62_001896"
     CDS             10878..11840
                     /locus_tag="IVM62_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP6845859.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11951..12289
                     /locus_tag="IVM62_001897"
     CDS             11951..12289
                     /locus_tag="IVM62_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP6845860.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12368..13075
                     /locus_tag="IVM62_001898"
     CDS             12368..13075
                     /locus_tag="IVM62_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP6845861.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13178..14617)
                     /locus_tag="IVM62_001899"
     CDS             complement(13178..14617)
                     /locus_tag="IVM62_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6845862.1"
                     /translation="MVIQKNNCSLQELSNDLHIPKRTIKELIRKINVSLKQFLLIDEF
                     IYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLE
                     QLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGN
                     DWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITEL
                     MDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFS
                     TSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLC
                     FPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIH
                     LQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTT
                     HNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4647 a         2519 c         3147 g         4308 t
ORIGIN      
        1 gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc
       61 atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt
      121 tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc
      181 atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc
      241 cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta
      301 ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg
      361 tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc
      421 cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac
      481 tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg
      541 atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct
      601 ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg
      661 tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg
      721 gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag
      781 ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag
      841 aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga
      901 tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga
      961 gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga
     1021 cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat
     1081 atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt
     1141 tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat
     1201 tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag
     1261 gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca
     1321 ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat
     1381 ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc
     1441 ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga
     1501 tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc
     1561 aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa
     1621 tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc
     1681 agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc
     1741 taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat
     1801 tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt
     1861 actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc
     1921 attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct
     1981 atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag
     2041 tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg
     2101 ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg
     2161 agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag
     2221 gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg
     2281 gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt
     2341 tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt
     2401 cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg
     2461 ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt
     2521 ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag
     2581 ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa
     2641 tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac
     2701 cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg
     2761 ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac
     2821 tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac
     2881 aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg
     2941 ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa
     3001 tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa
     3061 aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg
     3121 atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga
     3181 ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct
     3241 gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg
     3301 aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg
     3361 tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa
     3421 agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa
     3481 agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa
     3541 gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat
     3601 cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact
     3661 tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt
     3721 tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt
     3781 ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg
     3841 actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca
     3901 attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta
     3961 gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa
     4021 gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt
     4081 gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa
     4141 agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg
     4201 aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat
     4261 tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat
     4321 cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg
     4381 gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa
     4441 acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt
     4501 ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc
     4561 tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca
     4621 actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta
     4681 cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta
     4741 atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt
     4801 ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt
     4861 gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc
     4921 attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc
     4981 ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag
     5041 ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc
     5101 atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc
     5161 aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg
     5221 tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt
     5281 gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc
     5341 attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca
     5401 gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa
     5461 taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta
     5521 agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt
     5581 tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct
     5641 ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca
     5701 aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt
     5761 gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt
     5821 tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa
     5881 catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga
     5941 aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta
     6001 gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt
     6061 tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt attctggcta
     6121 tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc
     6181 aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat
     6241 agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga
     6301 catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc
     6361 ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat
     6421 atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc
     6481 gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta
     6541 acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta
     6601 ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa
     6661 gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt
     6721 gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat
     6781 ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct
     6841 atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta
     6901 gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa
     6961 atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt
     7021 gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga
     7081 aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa
     7141 aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat
     7201 ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa
     7261 aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc
     7321 actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat
     7381 cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat
     7441 cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa
     7501 ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac
     7561 aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt
     7621 gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc
     7681 ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt
     7741 gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga
     7801 ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca
     7861 atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc
     7921 cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca
     7981 gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc
     8041 tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag
     8101 catccctcgc tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca
     8161 aaagtcgaac gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat
     8221 gtggtagaag aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata
     8281 tgtaaaactc tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg
     8341 aacgatcact tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt
     8401 cctggaatgg attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt
     8461 ggaataaaac tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa
     8521 gaagaagtaa ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat
     8581 ttagaaaaga tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa
     8641 gctaagcaga gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct
     8701 gtattatctg ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt
     8761 agcagctata ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac
     8821 agagaaaacg cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa
     8881 ttgaaatgga aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga
     8941 tagacttaac tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg
     9001 accacatagg agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg
     9061 ttgagattgc tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg
     9121 gaaaacatgc gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct
     9181 atgaagtaga cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag
     9241 aaggaaaaac gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg
     9301 cagaagaatt cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc
     9361 cagaagatgc agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt
     9421 acaatacttt ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata
     9481 gtgaaggaaa aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca
     9541 aaacatgatt tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta
     9601 agaagcatcg caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat
     9661 gcagaaaatg tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc
     9721 aaattaaaaa aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg
     9781 gacgttttag gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg
     9841 atgactaaac atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac
     9901 cgggcggaag tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga
     9961 ttcaatacga ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc
    10021 acctcttatg atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg
    10081 atcaaaaaag atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat
    10141 ttgcagcaga cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg
    10201 cgttttccag tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt
    10261 ttagggaaat cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta
    10321 aagaaaacaa tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc
    10381 atgccggtcg tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg
    10441 aaagcgatgc agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata
    10501 tctggaaata tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag
    10561 ttcaaattcg tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta
    10621 cttagcgaaa tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat
    10681 atcttgattg aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta
    10741 gacattcgtc cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg
    10801 tttattgaaa acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct
    10861 tagaaagcag gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg
    10921 ataaaatcat tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag
    10981 gattagccga actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa
    11041 cgattgaagg gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag
    11101 gagcgctagt catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc
    11161 agttggcaga aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc
    11221 ttgtgtgggt aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga
    11281 tgtttcctga atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg
    11341 tcggagatac agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt
    11401 cgatattgag aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc
    11461 cgatgaaagt cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg
    11521 gtgcaggaag aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag
    11581 agacagcttc gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt
    11641 ttgttcagca gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca
    11701 atgaattggc taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa
    11761 aagatttagc agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata
    11821 agagcagaaa aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca
    11881 aatgtatctt ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag
    11941 gagaaagaag atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga
    12001 tcgaggcttg ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac
    12061 tgtcatagaa gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga
    12121 tagcgtgaca gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg
    12181 aacaaaagtc aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga
    12241 aggatttgga ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa
    12301 ggtcttttca cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga
    12361 aacgaaaatg tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat
    12421 tcttggatta gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata
    12481 cgatcaagtg agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca
    12541 aagtcaacag ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta
    12601 caattcaggt aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc
    12661 tgcgataggt gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt
    12721 cccattgaag attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt
    12781 ccccttgatt atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat
    12841 tttcgctttt tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc
    12901 gtctcggcga gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc
    12961 gatggcaaga cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac
    13021 attcctgcag ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa
    13081 agaagttgtg aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac
    13141 catttatttg gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct
    13201 cataaccttt tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg
    13261 tattgtgcgt tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat
    13321 ctgtaatgat caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa
    13381 ataactgctg caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca
    13441 aatggatgag taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa
    13501 ggtattgact gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc
    13561 ttaatttctt ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa
    13621 gtgtagaaaa actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa
    13681 gaaaataaaa ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta
    13741 agtaagtcat caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa
    13801 aaccaatctc ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat
    13861 aattaaagta ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag
    13921 taaagtattc tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag
    13981 aagaagtttt tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca
    14041 agtagatatt tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt
    14101 ttagtgcatt taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa
    14161 gaaaaaagta aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt
    14221 ttacagatag gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt
    14281 tttcgagata ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag
    14341 tttgtggata attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc
    14401 ataattttaa cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga
    14461 tctctccttt atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata
    14521 catttatttt tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata
    14581 attcttgtaa agaacaatta tttttttgaa tgaccatttg t
//
LOCUS       DACCIQ010000060        14384 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCIQ010000060 DACCIQ010000000
VERSION     DACCIQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.062"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..302)
                     /locus_tag="IVM62_001900"
     CDS             complement(<1..302)
                     /locus_tag="IVM62_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6845863.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQ"
     gene            complement(302..532)
                     /locus_tag="IVM62_001901"
     CDS             complement(302..532)
                     /locus_tag="IVM62_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6845864.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            819..1157
                     /locus_tag="IVM62_001902"
     CDS             819..1157
                     /locus_tag="IVM62_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845865.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1362..1937)
                     /locus_tag="IVM62_001903"
     CDS             complement(1362..1937)
                     /locus_tag="IVM62_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP6845866.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2546..3127)
                     /locus_tag="IVM62_001904"
     CDS             complement(2546..3127)
                     /locus_tag="IVM62_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6845867.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3310..3936)
                     /locus_tag="IVM62_001905"
     CDS             complement(3310..3936)
                     /locus_tag="IVM62_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6845868.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3958..5289)
                     /locus_tag="IVM62_001906"
     CDS             complement(3958..5289)
                     /locus_tag="IVM62_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP6845869.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5444..5845)
                     /locus_tag="IVM62_001907"
     CDS             complement(5444..5845)
                     /locus_tag="IVM62_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6845870.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5931..6137)
                     /locus_tag="IVM62_001908"
     CDS             complement(5931..6137)
                     /locus_tag="IVM62_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845871.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6232..7734)
                     /locus_tag="IVM62_001909"
     CDS             complement(6232..7734)
                     /locus_tag="IVM62_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP6845872.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7752..8207)
                     /gene="argR"
                     /locus_tag="IVM62_001910"
     CDS             complement(7752..8207)
                     /gene="argR"
                     /locus_tag="IVM62_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6845873.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8305..8922)
                     /locus_tag="IVM62_001911"
     CDS             complement(8305..8922)
                     /locus_tag="IVM62_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845874.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9143..10516
                     /locus_tag="IVM62_001912"
     CDS             9143..10516
                     /locus_tag="IVM62_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6845875.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10591..11703
                     /locus_tag="IVM62_001913"
     CDS             10591..11703
                     /locus_tag="IVM62_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP6845876.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11775..13412)
                     /locus_tag="IVM62_001914"
     CDS             complement(11775..13412)
                     /locus_tag="IVM62_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP6845877.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(13580..14188)
                     /locus_tag="IVM62_001915"
     CDS             complement(13580..14188)
                     /locus_tag="IVM62_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6845878.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3916 a         2996 c         2542 g         4930 t
ORIGIN      
        1 tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc aagagatttt
       61 aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga gtaacgtgtc
      121 ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt tttacggtta
      181 aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt tcctacggag
      241 ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct ttcttctgac
      301 actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc tcataatact
      361 cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta attgttccat
      421 catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg ttatcagaag
      481 gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc attatcatca
      541 ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc tttgtaaact
      601 atctagaaaa ttgttttgtt taaagtatat cttattggat ataaatagaa ataaattgta
      661 gccagttcat tttttcttta actaaaaaaa taatccaaga aacattgaac ctatggatcc
      721 aaaagctacc caaatactat agctaacccc ccgctacctt caattttgca gataaaacgc
      781 tttgggagaa aatttcaaag acaacaggcc atcctatcat gcccgattat aaacaaaaag
      841 ctggctattc cttttgctct gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg
      901 aagaagtttc aactgttcgg atcaaagaat gcccgcttca aatagaaaca ttggttcaac
      961 gaatttcaac tagacaagac tttgctattg tagaacgtga gatcgtagct atttttgtag
     1021 aagaagggat tttacatgat ccaactcata tcaatgtgga taaatggcaa cctcttatct
     1081 ataaattcag agaatataca actgccaacc agtcgttaac tttgaatttt agattccaag
     1141 aattcaaaga gtcataatga taaacatcgt taggtataaa taaaaaagac tcgcagcacg
     1201 agtaaaaaac aaatagaagc tatacattag aaaggtcgac tgtgacccta tagatgcagt
     1261 atatgcagct tatgagcatt tcttctgttc tctttgtttt tgggatctgt gctgaagtct
     1321 ttttcgtttt ttgccgctct ttctttgaaa aatcgattat gttatacctt ctccattact
     1381 aattcaggca tctgctgagt aatgattttt cgcgcaaaag aaatatctgt tacccaaggt
     1441 tcgatttgtt cttcagtcaa gacaagcggc attcggtcat ggattggtgc gactgtgcgg
     1501 ttaggttcag tagtcattat gattgattct gccatttttt catcctgatg gctacgataa
     1561 aaaccgccaa tatacactat tttttcctta tttgaaaaca taaatttgtc tttttcttca
     1621 ttccactcat aaaagccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
     1681 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
     1741 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
     1801 aacgtcacaa caagatttga cggaaaaact tccccagctg ctaattctgc tgcctgcttc
     1861 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
     1921 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
     1981 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
     2041 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
     2101 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
     2161 cttacgacct ctgggtaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
     2221 aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc
     2281 tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa
     2341 aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg
     2401 ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg
     2461 aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     2521 ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt
     2581 tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga
     2641 tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca
     2701 gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta
     2761 attctagtag caacagacgg tattcttcat ccactgtact caactcaata atcaaattac
     2821 ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca
     2881 tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg
     2941 tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa
     3001 agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg
     3061 tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg
     3121 ttttcaatga tattctcctt tttcatttct catagtccta tattatacag taaattcatc
     3181 attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact
     3241 ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca
     3301 attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat
     3361 aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc
     3421 tgtcgctacc acaacattga atccactctc tatcaattca cgcatatggg aatcgctaca
     3481 caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag
     3541 cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc
     3601 atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc
     3661 atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc
     3721 aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc
     3781 tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa
     3841 gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc
     3901 agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta
     3961 ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa
     4021 taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga
     4081 atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt
     4141 gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt
     4201 gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga
     4261 taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt
     4321 attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa
     4381 ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta
     4441 ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag
     4501 tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac
     4561 ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc
     4621 tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg
     4681 cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt
     4741 agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat
     4801 cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg
     4861 acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat
     4921 ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga
     4981 aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata
     5041 atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc
     5101 gattcctctt tctccattga aaatcaatga tttagaagga atacagccga tgttgataca
     5161 tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc
     5221 taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt
     5281 ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc
     5341 gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc
     5401 ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt
     5461 ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg
     5521 caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga
     5581 tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg
     5641 ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac
     5701 ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt
     5761 tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca
     5821 ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg
     5881 gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat
     5941 accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac
     6001 agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg
     6061 attttaagat acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata
     6121 accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa
     6181 ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc
     6241 tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa
     6301 cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct
     6361 taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc
     6421 ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc
     6481 taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg
     6541 tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt
     6601 taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata
     6661 gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag
     6721 acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg
     6781 gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac
     6841 gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt
     6901 tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa
     6961 aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc
     7021 tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga
     7081 acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc
     7141 atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat
     7201 ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc
     7261 tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc
     7321 ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag
     7381 atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat
     7441 aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag
     7501 actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc
     7561 aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag
     7621 tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg
     7681 taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa
     7741 gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct
     7801 tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg
     7861 taagccactt catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg
     7921 ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa
     7981 cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt
     8041 tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc
     8101 acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt
     8161 tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct
     8221 caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt
     8281 tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt
     8341 cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga
     8401 agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgcaggttc
     8461 ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa
     8521 tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc
     8581 tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt
     8641 tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata
     8701 gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc
     8761 atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc
     8821 gatccctctg atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt
     8881 catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa
     8941 ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca
     9001 ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc
     9061 ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg
     9121 aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt
     9181 aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac
     9241 tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac
     9301 gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt
     9361 tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga
     9421 tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac
     9481 aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg
     9541 aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac
     9601 ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc
     9661 cgttttgatt ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa
     9721 aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct
     9781 aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat
     9841 tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc
     9901 tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt
     9961 attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc
    10021 ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt
    10081 ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct
    10141 ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt
    10201 gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac
    10261 caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac
    10321 cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt
    10381 ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt
    10441 aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt
    10501 tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga
    10561 aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg
    10621 atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt tgctttattt
    10681 gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg
    10741 aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa
    10801 cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt
    10861 cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg
    10921 ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta
    10981 ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat
    11041 ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc
    11101 actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct
    11161 gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat
    11221 gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg
    11281 agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat
    11341 ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact
    11401 agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag
    11461 ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc
    11521 ccattttttg gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct
    11581 tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt
    11641 cattggtcta cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca
    11701 tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg
    11761 aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca
    11821 tctagcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt
    11881 tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt
    11941 aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt
    12001 ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta
    12061 ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct
    12121 tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc
    12181 tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa
    12241 gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg
    12301 atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa
    12361 cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct
    12421 cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga
    12481 tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa
    12541 ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc
    12601 gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc
    12661 gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca
    12721 ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa
    12781 cgtaatccta gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga
    12841 aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa
    12901 acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa
    12961 gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct
    13021 tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca
    13081 gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc
    13141 cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca
    13201 gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga
    13261 ctcccttttg ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg
    13321 taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt
    13381 tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa
    13441 acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca
    13501 aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca
    13561 tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat
    13621 cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg
    13681 aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag
    13741 tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag
    13801 ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa
    13861 cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt
    13921 tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa
    13981 tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc
    14041 ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat
    14101 cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac
    14161 ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc
    14221 tttcgacgct attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt
    14281 taatgacaaa ataacgaatt atttaatatt tgtaacattt aaacaaatat ttttttgata
    14341 caaaaccata aaagccttgt tgtataagca aagtaatcgt tttt
//
LOCUS       DACCIQ010000061        14304 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCIQ010000061 DACCIQ010000000
VERSION     DACCIQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.063"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(67..1047)
                     /locus_tag="IVM62_001916"
     CDS             complement(67..1047)
                     /locus_tag="IVM62_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6845879.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1071..2555)
                     /gene="galT"
                     /locus_tag="IVM62_001917"
     CDS             complement(1071..2555)
                     /gene="galT"
                     /locus_tag="IVM62_001917"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP6845880.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2567..3556)
                     /gene="galE"
                     /locus_tag="IVM62_001918"
     CDS             complement(2567..3556)
                     /gene="galE"
                     /locus_tag="IVM62_001918"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP6845881.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3804..3971
                     /locus_tag="IVM62_001919"
     CDS             3804..3971
                     /locus_tag="IVM62_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP6845882.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4033..5844)
                     /locus_tag="IVM62_001920"
     CDS             complement(4033..5844)
                     /locus_tag="IVM62_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP6845883.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5841..6206)
                     /locus_tag="IVM62_001921"
     CDS             complement(5841..6206)
                     /locus_tag="IVM62_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP6845884.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6369..6764)
                     /locus_tag="IVM62_001922"
     CDS             complement(6369..6764)
                     /locus_tag="IVM62_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6845885.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6782..7744)
                     /locus_tag="IVM62_001923"
     CDS             complement(6782..7744)
                     /locus_tag="IVM62_001923"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP6845886.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7744..7956)
                     /locus_tag="IVM62_001924"
     CDS             complement(7744..7956)
                     /locus_tag="IVM62_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP6845887.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7977..8675)
                     /locus_tag="IVM62_001925"
     CDS             complement(7977..8675)
                     /locus_tag="IVM62_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6845888.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8695..9237)
                     /locus_tag="IVM62_001926"
     CDS             complement(8695..9237)
                     /locus_tag="IVM62_001926"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP6845889.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9369..10373)
                     /locus_tag="IVM62_001927"
     CDS             complement(9369..10373)
                     /locus_tag="IVM62_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6845890.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10370..11359)
                     /locus_tag="IVM62_001928"
     CDS             complement(10370..11359)
                     /locus_tag="IVM62_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6845891.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11356..12162)
                     /locus_tag="IVM62_001929"
     CDS             complement(11356..12162)
                     /locus_tag="IVM62_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6845892.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12328..13284
                     /locus_tag="IVM62_001930"
     CDS             12328..13284
                     /locus_tag="IVM62_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6845893.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13361..13879)
                     /locus_tag="IVM62_001931"
     CDS             complement(13361..13879)
                     /locus_tag="IVM62_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6845894.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13967..14116)
                     /gene="rpmG"
                     /locus_tag="IVM62_001932"
     CDS             complement(13967..14116)
                     /gene="rpmG"
                     /locus_tag="IVM62_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6845895.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         4275 a         3119 c         2468 g         4442 t
ORIGIN      
        1 tttgcccctt cctgaaacac aaaaaagctg cggtatcact tcatttcccc acagcttttc
       61 catcttttat ttcgttgatt ctcgaataat caactctgtt tccacaataa tctttcttga
      121 aaccggtgct ttggaattac ttaattgaag aatcgtctcc actgctagct ctcccatcca
      181 ttcggtataa acttttacag tagaaagaga gggcgttaca tatttcgcta cactgacatc
      241 attgaaaccc atcacagaaa tatcttctgg taccactaaa cctttttctt gaatcgcttt
      301 cagtgcgcca atcgctaatg catcattagc tgcaaaaagc gcatcaggaa catgcggatt
      361 cgtttttaaa tactgcttca ccgcttgata tcctgactcg acagaaaaat ctgcttcgat
      421 aattggacct tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca taattctggg
      481 atcttttagc cgctgatggt ttttcttcgt atattctgtt ccagcaagca tagcgatatg
      541 ttgcgcttta tgggagacaa aatgttcaat gactttttga acacttgact taaagtcgac
      601 aactagagag tcgattcctt gagaagtgcc gtcagaatct accaaaagca aggcattttg
      661 ggatcctttc atttctaata actgagcttc gtcgaatttt cctagtgcga tcgttccatc
      721 tgtcggaaga tcactaatgg aatcccacgt ttctttcaat aaagtaacgt ttgcttcttc
      781 tgcctttttt tcgattccta gacgaataga taaataataa agatcttcaa gctcttccgt
      841 atcgtcgtac cactggatca ggcgaagcgt ttgcttagta tttgctgcat ttttacgatg
      901 tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg ctaagccaac
      961 ggaaagatca ggatcatagt tgagaactct tgaaacagta gcaggagaaa ctcctgccaa
     1021 ctgcgcaatg tctttaattg ttgccatttg tctctcctcc ttttttacgt ttataaaatt
     1081 gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac tccagcgtct
     1141 tccagtacac gtgcaaaaac ctgacctaca gatttttgta cgacatcggt gaccgtatct
     1201 ttcgtcaaat caggatgttc tttttttaat tgttctgccc acggacgatg atattcagcc
     1261 atctggtttt cttctcctaa taagaaatgc tctacttcta ctagttctgg tttcaaacga
     1321 ggtggtaaaa cagcaagccc cattacttcg atcaacccaa tgttctcctt tttgatatgt
     1381 tggacatctt tatgtggatg aaaaatccca tcaggatgtt cttctgaaac attgttgtct
     1441 cgaaggacaa ggtccaactc aaagtaattg tcccttcttc gtgcaattgg cgtaatcgta
     1501 tggtgtggtg taccatctgc tgaaaaagct ctgatagata ctgtttcatc cgaatatgct
     1561 ttccactttt ccaatactga gttagctgct tcaatcaaca attttttctc tgttgattgc
     1621 aaacggatca cagacatcgg ccatttcacg actcctgcga tgacttgcgg ataatctgct
     1681 agtttgataa gtttttctat cggtgctttt gccataggaa attcatgacg tcctgcctga
     1741 taatgatcat gagaaagaat cgatcctcca acaatcggca agtcagcatt cgaaccaaca
     1801 aagtaatgag gcaagacttc agtaatccga agcagtctag caaatgtttc ctttgtgatt
     1861 ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt cattgtaata agcataagga
     1921 gaatattgga atccccagct ttcaccgtct agattcattc gaataatccg gtgattggtg
     1981 cgggctggat agttcaagcg acctctatat ccttcgtttt ccatgcaaag catgcaggta
     2041 ggatagtcta cttttaccgc ctcgcgttct gctgcgattt gttttggatc tttttctggc
     2101 ttagataaat tgattgtgat ttccagttca ccatactccg actctgccgg aaatttgatg
     2161 ttttttgcga tggctcttgt tttgatgtag tcatttttct tacttaaatt aaagaaatac
     2221 tcagttgctt cctctgggct tttcgaatag tgctgtgcaa agaatgcatt gacgactgaa
     2281 ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc tgctggcaat
     2341 tctgaaagaa caccattttc tcttgcctta gcgacaagct gatccaataa gtctacagat
     2401 tcttccttaa ctggtcttac ttcaatgctt tctagagcat cttctcctac catccctaaa
     2461 agacgattct gtaaatataa gcggtccatt tccatccatc cgccagcttc gatcgcaagt
     2521 gtggcaaaat cacaaatcgt ttgactgatt gacatatctc attcccctat tcttcataac
     2581 catttggatg gctgacatgc caatcccatg ctgttttgat aattgctttg atatctgtgt
     2641 attcaggttt ccaacccaga acctctctcg ctttttcact tgaagcgact aatcggctag
     2701 gatctccagc tcttcttggt gctatttctg ctggaatttc ttttcctgtc acttctctag
     2761 ctgcttctag catttccttg actgagtatc ctttgctgct gcctaaattg aagaagttgc
     2821 tttcatttcc ttctttcaag tattccaaag ctaaaatatg tgcagcaatc aaatcttcta
     2881 cttgaacata atctctgata catgttccat ctggtgtatc ataatcgtct ccataaacag
     2941 ccaaggcttt tctttgacca agagctactt gtaaaatgat tgggacaaga tgcgtttctg
     3001 gtgtatgatc ttctccgata gatgcatctg cttttgctcc ggctacattg aagtagcgca
     3061 aagcgacata gcgcatgcca tatgcttggt cacaccactt catcattttt tccatcatca
     3121 atttactttc accatacgga tttttaggat tagctggtgt atcttctgtg attggagact
     3181 ctttcggctc tccataagtt gctgcagtag aagagaatac gattttattc acgttgaact
     3241 cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa tacatcaatg
     3301 gtttttcgac agattctcct actaaagagc ttgcagcaaa atggattact ccttcaatcg
     3361 gttccttttc aaatacggaa cgtagaaatt ctttatcccg gatgtctcct tcataaaaat
     3421 gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa attatctacc acagctactt
     3481 gatacccctt ttgaactagc tggtctacag cgtgcgaacc gatataaccg gctccgccta
     3541 gtactaaaat tgtcataaaa aacactcctt tgattagtaa aattgtgttt gttgattcta
     3601 tataatttaa aaaacgaagt ttacttttat ggtgaaaaca aacaaagtaa aatttcgtaa
     3661 attcatatgg taaatttata ataaaacatt tagtaaacat tttcaaggat tttttgcaaa
     3721 ggtttacaaa caaacgtaaa actagccttt ttttctcttt tgttccataa tagatataac
     3781 aaataataaa tgaggtggat tcaatgaaaa aatttgtatc tggaatatta gtaggaagtc
     3841 tggcaacagc cgctgccgtt gctggtttgg ttgcttctgt gaaaaaaaca gtgatcgatc
     3901 caattgacga aaaagaagca atgatcgaag aaaaccgtaa aaaagcaatg cgtaaacgcg
     3961 tttctcgtta ataattattt aaaaatacgc atgaagactt tctaaaaaac agataagtca
     4021 tcatgcgtat ttttatttgt cagtcaatat aagttgtgct attttatctg cctgtctcag
     4081 attccatgga tctgtataag gtctagctac tgctgcagtt agccaatcca caccttcaac
     4141 aggtattcca tagcaataaa agtcagactg gatttcccct gaagcatcaa tcacgcgatt
     4201 cgtacctcgc tcaacatcta tagcttctga atgataactt cctgtattat ctgaaaatga
     4261 aaaagggcga acaagtttgt tttccttcaa attttttatg acaggattac ttgaacgaga
     4321 aaagcttgtt tgtggcaatc gagcttcaat caaatagcga ctgctgattt ctttatcttg
     4381 tagagaaata acatagcggt tgtgcttttg ctgaatatca aatggcggtt cgatcaaatg
     4441 aaaaataccc gcatctatca gtgctgctaa ttctttcgtc cgaatgattg gcggaccgat
     4501 cgttaaaaag ctatttagat gagtaaacca tccccatagt atttctttgt attccttagc
     4561 agaaaaaacg tgccattcaa tagcctgccg gattggatca cgccagtctt ttaatgcatc
     4621 aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc gcttctttta tctgcccttc
     4681 aataaatatt cggctagctt ctgaaaaggg ctcattctga taggcaagag gagtttctaa
     4741 atacgaccaa tcccatctat aagggattag tgctggatgc tttccgagac actcctctga
     4801 agagagggag agaaaatcat gctcaaaatt tttctttgga atcggcaagt gatattcttc
     4861 gatgattttc ttgtaataaa acaactcaac gtcttttttc agatagtcaa aaaatgtttc
     4921 gccagtcaaa aatcccgact gatgccattg ctccaggttt tcttttgtta gtaaccgtgg
     4981 ccatgccatt gctccgcctt gcttttgatt tcttcctcga gggtaataag ggagccccct
     5041 tcttgatcca ctgtatacaa tcggttcctt gcctgatgga tgatagacca gttttccctt
     5101 ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat ccgacataat caaaaaaact
     5161 taagcctaga cctcttaaaa agacattttc tttaggcggt atcttcgaaa gatctacatc
     5221 tgcaggatta gaaggaagct gatacagtaa aacatttttc tttgcgtagt ctacccacac
     5281 tctttcttct tctgtctcat cattttccca gtgaccgctt gctaaaacaa gctgatcgat
     5341 agaatagctt tgctgatctg tctgtaaaag ataaccatct tttcttttgc ttactttttt
     5401 tactaaagaa taaacaaact gaatagaagc tggaaattct tcttgcaact ttgagaaaaa
     5461 ccagcgttga tagagtccgt ataaacatcg agtagattgc tgatcttttt ccagacgatt
     5521 gatttcttct ataattagtt gcttattttt ggttaccgtc tgttcgatat aatctgtagc
     5581 aggtccttta ctccattgaa acaagttggg tccctgtgtt accaatcctc cactggacaa
     5641 tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa ttcattaata actccggtgg
     5701 ctgattcaac cgccaaactc gacctccagg accattggga tcgaaaagaa aaagttcaag
     5761 agaatgtctg tcctgattat tatggagaat cctttctgct acacttaatc ctctaggtcc
     5821 ggcaccaatg atccctattt tcatttgcat gcctccattt cgataaattt tttcaataaa
     5881 acagcatggt tctgatgttc attttttgca ccataaacaa aagtaatgac tggatgttct
     5941 ttcataagtt gactgagttt aaaaatagct tgcttctttt cttcttcgaa aatcaattct
     6001 tgctggtatt tctttgcaaa tgaagcaaat cgttctggta catggtcaaa agttttgcgt
     6061 gcttcattac taggtgcaat ttctttcaac cacagatcta actgcgcttt ttcttttgaa
     6121 attcctcgtg gccagaatct atccacgagt attctatagc catcctcttt ttcagcatca
     6181 aggtaaacac gttttaattt gatcatagtt gtttccttct ttctaccttt tcgcttagct
     6241 tatatatgta gataatagca caaaataaaa aaaatgaacg gcacaactat ccattgcgcc
     6301 gttcatgtga tcttggtcaa aatctaatga ttgattcggt ttttaaagct gtcatacaat
     6361 catctgtttt atactttttt acttttgatt tttccagacc atccgtcata tccaccctta
     6421 agtaaataaa tctcttgata acctgcttta cgtaatttat ttgctgtacg gatgctcaaa
     6481 cttttctttt gatcgtaaat atagacaggc tggtcttttc gaatagatcc taatgaatct
     6541 ttcaaaacag tgtaagggat gtttcttgca cctaaaatat gcccagcatc aaacacatct
     6601 ttttcgcgaa catcgattac ttgtgctttt ctcattcctt ctcgaaattc ttcttctgtc
     6661 aaagtcgttg ctgaacgttt ggccattact cgtagataga cttcccaaag gatgattgca
     6721 agcacaatca atagcaaaat gatattaatt acccataaaa ccatgtaatg cctccgtttt
     6781 tttaagcaaa tttcaaggct aatgaggcag caccaatgac accagcttca ttaccaagct
     6841 ctgctaattt gatttttgtt gattctctta cttgcgggaa agtaaattct ttaaaatatt
     6901 tctctacacg gctacgtaaa aattctcctg ctgccgatac tcctccacct aacacgatac
     6961 ttgacggatt taatgtattc cccaagtttc cgcaagctaa acctaagtag aagcatacgc
     7021 gatcaacaac catcaatgca aacggatcgt cttcttgagc catttcaaag atatctttac
     7081 ttgttacttc ttctccgttg tctagcattg ctttcagttt tgaatcccct gcgtattctt
     7141 ctgccaagaa acgacctagg cgtaccacac ctgtagcgct agatacagtt tctaaacatc
     7201 cgcgtttacc gcaagtacat tcgaaccctt caggatccac agtgatatgt ccgatttctc
     7261 ctccagctcc gctcacgccg tgaatcaaat ttccgccagc aacgatacct ccgccaacac
     7321 ctgttcctag agtaacgaat acaacgtctg gatcgttttc tccagcacct ttccatcgtt
     7381 cacctaaagc agcaacattc gcgtcattat cgatagcgaa tttgattcct gttcctgcct
     7441 cgatcagttt tttcacaaat tgaacttctg tccagtttaa gttatacgca ccaataaccg
     7501 ttccagcttc gctgtccacg cttcctgggg tacccatgcc gattccgata aagtcttcgg
     7561 ctttcatatt atataaattc aaacgatgat tgattgattc aatgatttca ggaacgatat
     7621 gcttaccatc atccaaaata ttggtatcga tgctccattt ttgctgaatc tctccttctg
     7681 gtgttaaaat tgcaaatttt gctgttgttc cacctaaatc aatcccaata atcttcttgt
     7741 ccattacaaa tttcctttct ttcggctttc ttcaattcgg tgctctcttt ttaatatgcg
     7801 ccacgctgtc atgtatgtat catgatcaat caaacggcta tcatacagat tacgcaattc
     7861 gatagtcatt aattcaatgt catagatgcg tgcacccaca tagataaaga tgccaaattt
     7921 tttgaacagc tgctgtacat catataagct ctccatcttt aagcacttcc ttcatattat
     7981 acaagtaaac catatttttt taaacccagt aaaatacata ttacaaaaag aaatacaaaa
     8041 actagtgtag aaagaatcct ttggtgtatc gaatagcttg tctgttgttt cggtagaccc
     8101 aaaacattcc ctaacactaa tccgccaatt agcccaccga catgtcccca aacatctaca
     8161 gaggtatcca gaagaccaaa aacaaatgtc aacacaataa ataaaagaaa atgtctgact
     8221 agctgctgga tcgctgggtt atgtttaaaa tgaaacccta agataaacaa cgcaccgaac
     8281 aaaccgaata tcgaagtact tgcaccacca gacagaacat tcgcttcgtt aaaagcaaag
     8341 cttgctgtat ttcccattat cccgctgaac atataaatca agaaaaaacg ccaatggcca
     8401 tagattgcct cgatttgctg ccccataaaa tacagtatca gtgagttcat tacgaaatgc
     8461 atcagtccaa aatggatgaa gattggtgtc actaaacgcc accattcatt gaagtgaaca
     8521 agataaggac catacattcc ggcataataa gggatctcaa gtcgcggaaa taattccatc
     8581 atgaggaaga ccgctgtctg tatccctaaa aaagcatagg tccataacgg tcgtcttaac
     8641 catgcacgca attttatttg ctgttgataa ttcatgaaaa cctcccattt cattttagtc
     8701 aataaacaat tgttggatct tctgatcaaa gctctccggt tgccattgtt cattcaattg
     8761 ttcacgaaaa acaaggctac atgttttttt atgaaaatta tttaaatacc ggtcgtaata
     8821 accaccgcca aaaccgattc gataaccggc tctattgtag ataatacctg gtacaatgag
     8881 gagatcgatc atctccggca aatagagctt ctctgattgt ggctcaagaa caccaaaact
     8941 cgaacgtaca agaacggact gttcatccat ttcataaaac gccatctgac gtttcggcaa
     9001 agttttaggt acaactattt tcttttgttc tttttctgct cgttggataa ccggctgtgt
     9061 atccagttca aattcattcg ataaagtcgt ccctatcact tttgctgact tccagtactc
     9121 gctggcgaat agctgtttat agattcgctg ttctttttcc tttttcttgc cacttttggc
     9181 cagatttttc agcttttcga ttcctttccg gcgtaatctt actttcaatt ggtccaaaaa
     9241 ctcacccttc cttctctcat aaaatggtat aggaaaaaaa gaaaaatagc aatcgaactt
     9301 tcaaataaat gcataaatag aataaaaaga agacctagaa atgattggcc ttcctttttt
     9361 aatgaatttc aactgttcag cgcgtgtttt tgtctgataa aaagtgcata cagaaaatat
     9421 ggcgcaccaa aaagggcaat caaaattcct gtaggtactt ccccacttag tagaatcact
     9481 ctagcaacca tatctgcact aagcagaaaa atggaaccga acaaaccgct cagaacaaag
     9541 gtccagttat tttctttacg tacgactagc ttcgcagcat gaggagctag tagtcctaca
     9601 aaagataaac tacctgtcac cgctacacta ccgcttgcca aagcaacagc taacaacaaa
     9661 aggatttttt gacttttagc cacgttaagg cctaatcctt gcgcgcgttc ttgaccaaat
     9721 gatagcaata caatcctctg tgcataaagt cctcctagcg gaattaagat aaccgcccat
     9781 ggaagcaatg cccaaacata aggccagctc gcaccccaga ttgatcccgc taaccatgca
     9841 ttcacaaatc cataattttc cttagataat tggatcgtgc atagcaatgt taatgcagaa
     9901 aggccggcat taacggcaat accggacaat aagattcgat tcatacccaa aaagccattt
     9961 cgatcatagg ccgcaagata gacacagcaa gcagctaaaa caccacctag aaaggctaca
    10021 actagcaata gaaacggcag cgaatagtgc gaaaagaaac ctaaataaag catcactaaa
    10081 aatcctgctc cggcattaat gcccaatata ctagaatctg ccaattcatt tcttgttact
    10141 ccttgcaaga tataaccaga aacgccaaga caagctcctg ctaataaact gatcagtaat
    10201 cgaggaactc gaaattctct aacaatcagc gccaaagaag atgattcttt ccctatgaac
    10261 gtggcaaaaa gctgatccag gtaaataaaa ggcgtaccaa tcattaaact acctatagac
    10321 aggatgacaa gtacaaaaag caagaaatag aacgtccaat atattttttt caccgttgtt
    10381 ccctccttac ttgtatcagt aaaaacggaa cgccaatcaa tgcaatgact aatccaaaag
    10441 gtgtttcaaa agggggattg atcatcctcg ctgtaaaatc cgccaacata accaatccac
    10501 cgccaactaa aaaagagaca ggtagaacat aacgatagtc cgttccaacc attccgcgaa
    10561 caactggcgg aaccaacagc ccaataaatg aaacaggtcc gactaacgaa acggcaatgg
    10621 ctgctaacac tgcaactaga cagaaaacaa tcaaccgcgt tcgtttagga tgacttccaa
    10681 gagctaatgc atgtgtttca ctcaaatgaa tcaaagatat tgcttttcct aatgaaaaag
    10741 aaaccaaaac agcaagtata tataaaggaa acaatactgc taattgtttc catgtgatat
    10801 tggcagctcc gcctacaaac caaaaagcaa gatcctgctg cagattaaag aattgggtaa
    10861 gtgcttggct gatagcagta aaaaaagaac taatagctac gccagctaaa atcagctgta
    10921 aaggattcat gcccattttc gaaaactgca aaaaaccaaa tacgagcaaa gttacagcaa
    10981 aagaacctgc caatgacatt agaaacacag aaaaatcaga agctttaggc caaaaagcaa
    11041 aactaagggc cattgccaac gaagcacccg cgttgattcc tagtaagcct gaatctgcta
    11101 aaggatttct tgtcactcct tgcatcaatg caccgctcaa tgcaaaacaa ctccctacaa
    11161 gaaatgctcc cgccacacga ggcaaacgaa tatttcgtat gatttggtga ctttgttttg
    11221 atctatcaaa atcaaacaac gcctgccaaa aatctgcttt tcccacagtc gctgcgccat
    11281 ttgaaagagc aacaaaaaaa ataatcaaaa gaacaaaaca gaccaaaagc aggagtctat
    11341 ttttcttcat agtcctcata attgatcctc ctgctctttt ggcaacaaat cataggttag
    11401 aaaaactggt ttatttgttc ttggaagcgt gacaatcgtt gcatcaatct caaatatttt
    11461 tttcatacac tcttttgtga tcacttcttc aggtgtacct tctgtgatca acttaccgtt
    11521 tttcattgct aataaataat ctgaaaaacg tgaagcatga ttaatgtcat gaatcgacat
    11581 caagatcgtt gtttgtttca tttgattgat ttcagtcaat aactgtagga tctctaattg
    11641 gtgtgccgga tctaaaaatg ttgtcggttc atccaagatc aagatatctg tttcctgagc
    11701 aagcgccata gcgatccaga cacgctgggt ttgtccacca gacagcgttg cgatatcccg
    11761 catacgaaat ccagctagac ctgtagcatc caacgcccac tcaattgctt catgatcttg
    11821 cgtgtccatg ccactgaaca gtttttgata agggtatcgt ccgtaagata ctacttcttc
    11881 tactgtcata cctgtagggt gttcactagt ttgtgctaat aatgcaattt tttttgcaag
    11941 ttcttttgaa ctgtatgtag agatatcaga gttattgaca atgatctttc ctgatttcac
    12001 aggtagtata cgggtaaaac ttttcaataa agtagatttt ccacttccat ttggcccaat
    12061 caaactagtg attttccctt caggaatcgt aacagacatg ttttcaatga tttttttgtt
    12121 ttcgtagccg gctgcaatgt cagctgctct taatctcgtc atttcaccca tcctttcgct
    12181 gaaaatcatt ctcaattatg acgctgtttc aacatttcgt caagcgctac tttcatatct
    12241 atagccaaat gaagcggttg acagcgttcg aaatatcagg aataataacc aatgagaaag
    12301 attctcaatt atgttaggag gaaagaaatg aaattaaaaa caaaagtatt cacattatct
    12361 agcatattgc tgctggctgg agtactgagc gcttgctccc cctcttcaga taaagaaaca
    12421 gctacaactg ccaattcgac gacttcatcc aatagtactc atacagtcac agatacactt
    12481 ggacataaag tagagattcc aaacgcgccc aaacggatca tcggcagtta cttagaagac
    12541 tatctgattg cactagggga aaaacctatc gcccagtgga ctgttggcag tggttccatt
    12601 caagattatc tgcaagatga tttaaaggat gtcccaacga tttcttatga ccttccttat
    12661 gagaaagtgt taagctttga gcctgatttg ctgttgattt cttcttccgc tactgtcgaa
    12721 ggcgggaaat acgaacaata tagcaaaatc gcgccaactt atgttgtaaa aaacggaaca
    12781 gatgtcacat gggaagaaca actaaaagat gtcggcaaag ctttggcaaa agaaaaagag
    12841 gcagaaaaaa tcatttccga ttatcaaaaa tccatcaaga aaactcgaga agaactagca
    12901 gataaaatcg agaacaagac agctgcagtg ttgtgggtga ccaacaactc agcctttatg
    12961 gtcagcaaaa atcggtcaag tggaagaatc gtatacgacg atctggaatt tggtgttcct
    13021 gatctagtgg aagaggtctc caaaaaagcc acttctgact ggtctgctgt ttcctctgaa
    13081 aagctagctg aattagatgc tgactacatc atccttgtga acagtgacaa agatgctgcc
    13141 atgttcaatg aaccaagctg gaagaatcta aaagcagtga cagacaatca tgtactgcaa
    13201 ttcggacctg aatctagttg gctatataac ggacctattg ccagtcagga aatggtcaat
    13261 gatatcaaag aaaaactaaa ttgattttaa ctaaaaaaag catcttcacg attagtcgtg
    13321 agatgctttt tttcttatta acaaactaga cgcatagatt ttattcttct tttgaagcaa
    13381 ccctattttt tactctttcc catttttgaa aacgatgggc atatagattt tcttgatccg
    13441 ttatcccctc atactcctct ataagggcga agtctgaaaa atcaatattc ccaatatacg
    13501 tatcgccttc aaacgtgcta tcgatcaatg ttcgccagat ccgaccagtg taaggaagaa
    13561 gttcttgaaa aatccgacta ccacccgaaa catagatatc ttctgagatc gtttgactat
    13621 acgacaaaag ttcatcgatc gaatgcatta cttccgcatt ttctttcgtg cgaaaatttg
    13681 gttgcgttgt caacacgatg atatggcgat aaggaagtga aagatccccc attccttcat
    13741 aggtctttct tcccatcact aaaattcgat ccattgtgtg ttcgcgaaag aaacgcatat
    13801 catttggcaa tcgccatggt aacaaaccat ttttgccgat cagaccattt ttatcttgtg
    13861 cccacatgga aataaacata ttatccgctc cttatgattt tagatgtttt gagataaaaa
    13921 aagtctcaat tctttgacca gcaaggaatt gagaacttta aaaacattat tttgtttcac
    13981 ggtgcaaagt aactttgcgt tcgcgtgggc aatatttttg tttttccaaa cgatcaggat
    14041 tgttacgttt gtttttgctt gttagatagt tacgttcttt acaagaagta cactctaaag
    14101 tgatgtttac gcgcatgttt ttccctccca tttttacaag aatcctctaa gatgactctc
    14161 agacttgatt atcttaccat gattttttct agattgctag tgtttttcac gattttgtta
    14221 agtaaaacta cttgacgata aatacaacgg ttttacttct caaacaaaat ctttttcttt
    14281 ttgtataaat acccttacac ttgt
//
LOCUS       DACCIQ010000062        14221 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCIQ010000062 DACCIQ010000000
VERSION     DACCIQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.064"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(183..2180)
                     /gene="tkt"
                     /locus_tag="IVM62_001933"
     CDS             complement(183..2180)
                     /gene="tkt"
                     /locus_tag="IVM62_001933"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6845896.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2281..2532)
                     /locus_tag="IVM62_001934"
     CDS             complement(2281..2532)
                     /locus_tag="IVM62_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP6845897.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2678..3301
                     /gene="lexA"
                     /locus_tag="IVM62_001935"
     CDS             2678..3301
                     /gene="lexA"
                     /locus_tag="IVM62_001935"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP6845898.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3481..3699
                     /locus_tag="IVM62_001936"
     CDS             3481..3699
                     /locus_tag="IVM62_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845899.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3988..4500)
                     /locus_tag="IVM62_001937"
     CDS             complement(3988..4500)
                     /locus_tag="IVM62_001937"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP6845900.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4514..6811)
                     /gene="recJ"
                     /locus_tag="IVM62_001938"
     CDS             complement(4514..6811)
                     /gene="recJ"
                     /locus_tag="IVM62_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP6845901.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6953..7387)
                     /locus_tag="IVM62_001939"
     CDS             complement(6953..7387)
                     /locus_tag="IVM62_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP6845902.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7397..8185)
                     /locus_tag="IVM62_001940"
     CDS             complement(7397..8185)
                     /locus_tag="IVM62_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6845903.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8182..9123)
                     /locus_tag="IVM62_001941"
     CDS             complement(8182..9123)
                     /locus_tag="IVM62_001941"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP6845904.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9300..9887)
                     /locus_tag="IVM62_001942"
     CDS             complement(9300..9887)
                     /locus_tag="IVM62_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP6845905.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10108..11421)
                     /gene="obgE"
                     /locus_tag="IVM62_001943"
     CDS             complement(10108..11421)
                     /gene="obgE"
                     /locus_tag="IVM62_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP6845906.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11709..12719
                     /gene="gap"
                     /locus_tag="IVM62_001944"
     CDS             11709..12719
                     /gene="gap"
                     /locus_tag="IVM62_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6845907.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12804..13739)
                     /locus_tag="IVM62_001945"
     CDS             complement(12804..13739)
                     /locus_tag="IVM62_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6845908.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13925..>14221
                     /locus_tag="IVM62_001946"
     CDS             13925..>14221
                     /locus_tag="IVM62_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845909.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3974 a         3162 c         2510 g         4575 t
ORIGIN      
        1 cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat tctttgtttt
       61 gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc ttctaacaca
      121 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
      181 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      241 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      301 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      361 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      421 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      481 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      541 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      601 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      661 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      721 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      781 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      841 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
      901 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
      961 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
     1021 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
     1081 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1141 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1201 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1261 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1321 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1381 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1441 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1501 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1561 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1621 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1681 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1741 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1801 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1861 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     1921 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     1981 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     2041 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     2101 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2161 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt tacctttatt
     2221 tcttacactt tcattctata agatgtcaca cgaaaaagca atctttttca gtatttttta
     2281 ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt tggatttctt ttaatttttc
     2341 cggggtcacg tcattgccct caggatcgac gactttcatt ccttcgatat gatgacgcat
     2401 accggaacga aaagcttcca aatactcttt gcgcaagttg atttgttctt gttcttcggc
     2461 ttttgagaga ccattttcct tctttttacg tgcaagttca ttgatccgct ctattttttc
     2521 aggtgacagc atgctcagcc ctccttttac tctccttcat attatataaa aactgaaaaa
     2581 aaacaactag ataaactaaa taaaatacga acattcgttt gatttagcgc tttttatatg
     2641 gtactattta tttatcaagt gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag
     2701 acaaatcgaa gtcttacgat atatccatga acaagtaacc caaaaaggat acccgccgac
     2761 tgtccgtgaa atcggggaag ctgttcagct ttcttctact tctactgttc acggtcatct
     2821 tgcacggctt gaaaaaaaag ggtttatcca aagagatccg acgaaacctc gtgcaatcga
     2881 gttgacaaaa caaggtttag aaaagattgg gattcgtcca acaactattc cagtacttgg
     2941 cgtcgtaaca gctggagaac ccattttagc agtagaggat gcttcagact tctttccaat
     3001 ccctcctaat ctttcttccg aagaaggcag tctctttatg ctgacaattc gtggagatag
     3061 tatgatcaat gctgggatat tggatggtga caacgtgatc gttcggaagc aagaaactgc
     3121 tcaaaatggc gatattgtca ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt
     3181 ctataaagaa aaaaactttt tccgtctgca gcctgaaaac gacttgctcg aaccaatcat
     3241 cttagatcaa gtatctatat taggacgagt agtaggactt tatcgtagtc atatctatta
     3301 attgctacta attaaaagaa aactttttaa aaagagtttg agacgttggt ctcaaactct
     3361 tttttcgccc gggttcttct tctttcaaat ttttatcact tccctcttta caaaccgaac
     3421 aaatattcgt atactaaata tacgaacaat tgttcgcata aattaaagag gtgagaattc
     3481 atgaaatcaa tgagacgtct tggcttagta ttaactgttt tgtttttagg tattattatt
     3541 ggtaatttcg gcatacgtcc agctttactt gtgtttgttc cgttatttac tttgtggttc
     3601 atgttgtggg atgaaaagaa gtaccgtcaa tctgagcggc gcagaattca tgaagatcat
     3661 ttctatcatg agccctacta cagatccagg caatcataat aaggattgac tgctaattat
     3721 tcaaaagaca agaagttaaa cttttacggg ggtgtgacaa aaatcggtca catccttttt
     3781 ttcgaataaa cggtgttcta aagccagttt ttcggtatta agtcaaattg cggaaaaagt
     3841 gattttttaa acttctactg gtctttgctt gtttccattt ataaaaaaat agcgaccctc
     3901 aaaagtcaga tagagaatct gacttttgag ggtcgcttta aaatcgcttc tttaagaata
     3961 acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga tgtcatatcc
     4021 tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc agcctacgac
     4081 tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc cagtagctag
     4141 caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat gcaatgtcaa
     4201 tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag gtaacttccc
     4261 ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac atcctacgat
     4321 gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg cataatttac
     4381 gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg aaatatctcg
     4441 aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt ttaaatccac
     4501 tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg ctcaaaagca
     4561 aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc tcagtcaaag
     4621 gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta acaaatttca
     4681 attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc aaatattgag
     4741 ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc aatttaaaca
     4801 attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc
     4861 ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga tccgctaaat
     4921 catctggaca atccataaca actagagagt ttggttttct tttttcaatt attgccttta
     4981 cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc acgaatttca
     5041 acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc tgttcacgga
     5101 aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca gataccatca
     5161 gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc acatctacct
     5221 gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca tcaagctgac
     5281 tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt cttttgatat
     5341 tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta ccaaatggcg
     5401 ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc aatacttcat
     5461 caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata tattggttca
     5521 tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact gctgcatgat
     5581 gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa agattcaagg
     5641 cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta aggacaagcg
     5701 ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg ccttcatgcc
     5761 atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct aatgcttctt
     5821 gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat ttttttgctt
     5881 gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga cgtggatcgc
     5941 ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt gtttcgtctg
     6001 cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc cgttctgaat
     6061 gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta
     6121 aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt tcttccaata
     6181 atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat ggatagtgcc
     6241 ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca ggtagttcgt
     6301 gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc gcatcatttc
     6361 ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc ttttcttggt
     6421 ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat acttctactt
     6481 ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc ccatctgcgt
     6541 cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg attcggctga
     6601 ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc tttggatgta
     6661 aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac ttaccgacaa
     6721 atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc tctgtctcgc
     6781 ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag ttattatagc
     6841 aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga tccgctagac
     6901 agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc tattagtcaa
     6961 aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct ttcaattgac
     7021 tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac tccgcacgtt
     7081 cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt tgaagttctg
     7141 tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc tgccatagtg
     7201 ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg atgatcacta
     7261 agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt ttattgttca
     7321 atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca ttgctttgct
     7381 ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa tcaccaatcg
     7441 ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga ttgatttccc
     7501 gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt tctaaaacga
     7561 tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag aaatttgtct
     7621 cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat tctaatcgca
     7681 aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt gattttgcag
     7741 ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt ctttctgcca
     7801 tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact tcaaacatat
     7861 cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt
     7921 ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat tcgtccacgc
     7981 tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc aagcgtctgc
     8041 actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata acgattgctc
     8101 cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta cctgttacta
     8161 aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct
     8221 atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt tttcaagctc
     8281 taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc gttttgcctg
     8341 cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat
     8401 tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat tttccgcaag
     8461 cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc gtcctttttt
     8521 atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt ggatcgtttc
     8581 accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt ttagacgatc
     8641 cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac tgtcgatccc
     8701 atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc
     8761 atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac ctaaagagac
     8821 ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat ccccaccttg
     8881 aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt
     8941 aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt
     9001 gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt tcaatgctat
     9061 acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa attgtaattc
     9121 cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa gaagggataa
     9181 tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt
     9241 ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc tgttttttat
     9301 taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt gtccttccaa
     9361 gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg cacatctttt
     9421 tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat taccaattga
     9481 ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc tccttcaccg
     9541 attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt ggcatcttgt
     9601 gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg accacctaat
     9661 acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc ggtaaagtcg
     9721 ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac aactgccatc
     9781 tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa tgcctgatat
     9841 gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa aaacgcccct
     9901 ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag aaaagcactc
     9961 tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt tttccgaata
    10021 aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg aatctatatt
    10081 ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa actcaccgat
    10141 acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc
    10201 acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg tcatttggaa
    10261 taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac gatcgatttg
    10321 gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca gttcttcttc
    10381 gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca ataacgcgtc
    10441 caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag caaattcatc
    10501 ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg catccggcat
    10561 atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt tataagtaga
    10621 taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca tcccactcat
    10681 atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag
    10741 cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg caaaacttct
    10801 gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc
    10861 gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga cagatggaaa
    10921 acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc gctcttgtcc
    10981 tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa aacggatatt
    11041 ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt caattaaatc
    11101 tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta ctttaacata
    11161 ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac ctggctgcgc
    11221 tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat cgacgatcaa
    11281 gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag gaacgtattt
    11341 ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat cgatcgttac
    11401 ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt atttgactgc
    11461 ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct gattttgtct
    11521 atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt aaggtttcca
    11581 accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt agcagaataa
    11641 cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa
    11701 cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt
    11761 tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa
    11821 gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca
    11881 cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag
    11941 aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat
    12001 gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac
    12061 gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg
    12121 atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag
    12181 cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag
    12241 ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc
    12301 gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa
    12361 tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag
    12421 tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc
    12481 aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc
    12541 gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc
    12601 aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca
    12661 atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat
    12721 ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt
    12781 tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact
    12841 tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca
    12901 tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca
    12961 aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc
    13021 actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact
    13081 tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca
    13141 tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct
    13201 ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc
    13261 gattttccat tgttttctag cactttgttt atccactgta catagtcttt acgctctggc
    13321 catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg
    13381 acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc
    13441 atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc
    13501 gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa
    13561 ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg
    13621 tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac
    13681 actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata
    13741 aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct
    13801 ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta gaaaaataaa
    13861 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
    13921 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
    13981 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
    14041 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
    14101 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
    14161 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
    14221 g
//
LOCUS       DACCIQ010000063        13747 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCIQ010000063 DACCIQ010000000
VERSION     DACCIQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.065"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..863
                     /locus_tag="IVM62_001947"
     CDS             <1..863
                     /locus_tag="IVM62_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6845910.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="IVM62_001948"
     CDS             1189..2040
                     /locus_tag="IVM62_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6845911.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="IVM62_001949"
     CDS             2137..4017
                     /locus_tag="IVM62_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6845912.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="IVM62_001950"
     CDS             complement(4057..4959)
                     /locus_tag="IVM62_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6845913.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="IVM62_001951"
     CDS             5190..6593
                     /locus_tag="IVM62_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6845914.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /gene="ascB"
                     /locus_tag="IVM62_001952"
     CDS             6593..8026
                     /gene="ascB"
                     /locus_tag="IVM62_001952"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6845915.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="IVM62_001953"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="IVM62_001953"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP6845916.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="IVM62_001954"
     CDS             9311..11038
                     /locus_tag="IVM62_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6845917.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11110..11403
                     /locus_tag="IVM62_001955"
     CDS             11110..11403
                     /locus_tag="IVM62_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6845918.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="IVM62_001956"
     CDS             complement(11495..12355)
                     /locus_tag="IVM62_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP6845919.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="IVM62_001957"
     CDS             12447..12875
                     /locus_tag="IVM62_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6845920.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="IVM62_001958"
     CDS             13037..13213
                     /locus_tag="IVM62_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP6845921.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="IVM62_001959"
     CDS             13240..13686
                     /locus_tag="IVM62_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP6845922.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2348 c         3074 g         3827 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt atacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DACCIQ010000064        13174 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCIQ010000064 DACCIQ010000000
VERSION     DACCIQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.066"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            118..312
                     /locus_tag="IVM62_001960"
     CDS             118..312
                     /locus_tag="IVM62_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6845923.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            315..1517
                     /locus_tag="IVM62_001961"
     CDS             315..1517
                     /locus_tag="IVM62_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6845924.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2100..3731)
                     /locus_tag="IVM62_001962"
     CDS             complement(2100..3731)
                     /locus_tag="IVM62_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP6845925.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3745..5418)
                     /locus_tag="IVM62_001963"
     CDS             complement(3745..5418)
                     /locus_tag="IVM62_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6845926.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5431..5748)
                     /locus_tag="IVM62_001964"
     CDS             complement(5431..5748)
                     /locus_tag="IVM62_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6845927.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5773..7173)
                     /gene="lacG"
                     /locus_tag="IVM62_001965"
     CDS             complement(5773..7173)
                     /gene="lacG"
                     /locus_tag="IVM62_001965"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6845928.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7410..8195
                     /locus_tag="IVM62_001966"
     CDS             7410..8195
                     /locus_tag="IVM62_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6845929.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8198..8890
                     /locus_tag="IVM62_001967"
     CDS             8198..8890
                     /locus_tag="IVM62_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6845930.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9164..9535)
                     /locus_tag="IVM62_001968"
     CDS             complement(9164..9535)
                     /locus_tag="IVM62_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6845931.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(9935..10165)
                     /locus_tag="IVM62_001969"
     CDS             complement(9935..10165)
                     /locus_tag="IVM62_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845932.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(10259..10597)
                     /locus_tag="IVM62_001970"
     CDS             complement(10259..10597)
                     /locus_tag="IVM62_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845933.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(11525..11641)
                     /locus_tag="IVM62_001971"
     CDS             complement(11525..11641)
                     /locus_tag="IVM62_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6845934.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(12117..12995)
                     /locus_tag="IVM62_001972"
     CDS             complement(12117..12995)
                     /locus_tag="IVM62_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845935.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         4196 a         2680 c         2201 g         4097 t
ORIGIN      
        1 agttgggaag tcctgaacaa gtgatggatt ttcaagaaag taccgttcat tatacaaaaa
       61 taaagaaata acactattac ccaatgatag tgttttcttt gaaagtgagt ttatacaatg
      121 aaaaatacaa tttgtcgaaa agatttagag gctctaggat ttaaaacaca tcaagctacc
      181 acaataatcc gacaagcaaa aacaagaatg gtaaatcagg gatatccata ttataataat
      241 aaaagagttg gggtagtacc ccgacaagta attgaagaaa ttataggtgt tcctctttca
      301 caggaggaat aactatgaaa acaaaatacc cttgtgtgta tcaagataaa aatggtcgct
      361 tttcttatct agttgagtta ggaacagatc aagtgactgg aaagaggatc cagaagaaag
      421 gaacaaaaga ttctctagga aaacgattct catcagctcg tgaagcacac aaagaagtca
      481 tgcgtataaa aaatgaatac atggttgtaa atggatttgc gaattatgat ttaacttaca
      541 aagaatttat ggagcagtct tacgttccac actacaaagc tagcgtacaa aaaagtactt
      601 ggagttctcg tgagtgtgga ttgattcaga ttactgaatt tttcggtgat aagaagcttc
      661 gtgaaatcaa tgtgcgagat tgtgaaaatt atcgaatttg gttattgaac gatagcggat
      721 actcacaagc atacagttca cttatgtacg gtatgtttcg taaatcatta gactatgcgg
      781 tcaccctgca atttctgaat gagaatattt ccaaaagaac aaaagcaatt ccaaaaggaa
      841 aatcaatcgt tccttactgg acaaaagaag aatttgaaga agttttggca aaaatttata
      901 aagatgattt ctacgaacat atgtgttttt taatgatctg gctatattac atgactggtg
      961 ttcgtgtaag cgaaggatta gcacttaatt ggaatgacgt ggacttcaaa cgtaaaaagt
     1021 tgagggttca tcacaccttg gaaatgacaa atcaaaaaga ttttattcgg aaaccttaca
     1081 caaaaactga aagcggcttg cggatcattt ctctggacga tgatacaatt gaagtgctaa
     1141 aaaagtggaa agttattcaa aaaaatcatg gtgtagaaca atttattctt agctacactg
     1201 acctcccact ctatcgttca actgttcaac gaattattca gcgttatgcc aaactagcta
     1261 aggtacccct aatacaagga aaaggattac gtcattctca tgttagctac ctaatcaatg
     1321 agtttaacgc agacattctg gtcgtgtcgc aacggttagg ccattctagc cctgaaatca
     1381 ctttaaagca ctacgctcat ctatggagcc gtaatgatga aggaattgca gaacaaatga
     1441 caggaaacat taaatttacc tgtgcaacaa aatctaaagt tgcaaacttt aatggaaatc
     1501 aagcagtaaa aatctaaccc taccatttcc ctaccaaaat gaggaatagg ctgaaaaagc
     1561 actgatataa tagcttcttt acgaggctac tcatattgag tgggaataaa gagccaacat
     1621 aatatggagg gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc
     1681 attaaaaata agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt
     1741 cttatcataa aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca
     1801 tctaagtgct caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact
     1861 ttatgttatc tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact
     1921 ctctttagca taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat
     1981 gactggaaat cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct
     2041 tctacgctaa cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc
     2101 tattgtttca ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta
     2161 taaaccccaa tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc
     2221 cattcacttg ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat
     2281 aactgattag tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct
     2341 ttgcttatgc cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa
     2401 gttagtttgt agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt
     2461 ccttgttgaa accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa
     2521 cttgcatttt taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc
     2581 aaggcgtttc cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat
     2641 tgaacactga gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa
     2701 agaggttccc aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg
     2761 gcaagatcca gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct
     2821 gcttcggctt caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa
     2881 gctgtttcaa ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt
     2941 tgaagttcat tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat
     3001 gacacacctc gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca
     3061 tcagctaagt ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata
     3121 ccagacttca aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg
     3181 ccataaggcc agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa
     3241 acgtccagct gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc
     3301 atttcttcaa aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc
     3361 caaaaatcac tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt
     3421 gcaaggtcaa tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta
     3481 ttataaggat cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc
     3541 tctaaggcat cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct
     3601 gacaggatcg aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt
     3661 gaaacagccc ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt
     3721 cccattttca ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt
     3781 ttcaggatct ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc
     3841 taagcgatcg gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac
     3901 caccatatca aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact
     3961 gatcggtgct ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcgcacttgt
     4021 ccctgcgcca gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg
     4081 actcgctgcc acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat
     4141 cacctctgct ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg
     4201 tagatagatc aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc
     4261 catgcccgtt cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat
     4321 aaagctgttc atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg
     4381 tgttaacaaa aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa
     4441 tggttcgttg acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg
     4501 cttagactta gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc
     4561 catagttcct acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga
     4621 attgaacaat tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc
     4681 cacgatcgac ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat
     4741 agcgattcct aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc
     4801 ttctgcaaat gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa
     4861 tactgaaaaa gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc
     4921 tttaggcatt ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa
     4981 aaatgcagcg acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc
     5041 accttcaatc ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga
     5101 ggtggtgttg attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg
     5161 agcagtggtt gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca
     5221 tagccatgtg gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat
     5281 caaaatgaaa atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac
     5341 ggctcctaaa taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg
     5401 tttaatgatt ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat
     5461 aaagtcttga atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat
     5521 aaaaatgaat cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg
     5581 cgaattatgt gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc
     5641 aaagtttcct tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga
     5701 atatgcaacg atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc
     5761 cattatcact tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga
     5821 ttcttttggg taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt
     5881 atacccattt gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt
     5941 taccccggct gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct
     6001 cggctgatcc ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt
     6061 gatatagatt ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag
     6121 accttctgga taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg
     6181 ttctccgatt cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg
     6241 gattgcattt tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa
     6301 gtagtcatta cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata
     6361 gctggcctga tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata
     6421 gcctaaatag gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc
     6481 atgcttatct tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc
     6541 aatttcaccc ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat
     6601 catattatga agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac
     6661 acctaacaaa tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt
     6721 gacttctttg aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc
     6781 tcgattcaaa aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt
     6841 cacaaacggc gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat
     6901 tccttctgga tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa
     6961 tcttagacca tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata
     7021 aaaatcactt gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc
     7081 aacagcgcct ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc
     7141 gccaaaaata aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca
     7201 gttttcgctc tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa
     7261 caaacacaaa taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa
     7321 aaaccaccct aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat
     7381 agattatcct taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg
     7441 tagaaatggt caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg
     7501 tttctgatat gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc
     7561 gtgtccatgg aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg
     7621 aaaaagccac tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga
     7681 ttcacgaggg agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc
     7741 atttactgaa caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg
     7801 ctcaagcaga tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg
     7861 catttgtcgg accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta
     7921 tcagttcaaa tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag
     7981 cacagcagat cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat
     8041 tcaatcgaga tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg
     8101 atcgccagat ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg
     8161 ctgattcaac cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt
     8221 caacggaaca atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca
     8281 tgatcataca aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac
     8341 gaatacggag attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt
     8401 attgtcagaa gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct
     8461 tcagttgaca aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt
     8521 aacgattgcc acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt
     8581 agcccgctgg tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc
     8641 gcaaccggat atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct
     8701 tgttatcgaa gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat
     8761 tattgcaatt gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa
     8821 agatggaacc atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt
     8881 taattcttaa acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt
     8941 tataaaaaac tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat
     9001 agcagtgtat ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat
     9061 aattaacatt aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag
     9121 gagtactttt ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta
     9181 gttcctctcc accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg
     9241 aaataaacgt ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt
     9301 aagtatcatt aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca
     9361 tgcgtacaat tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac
     9421 taatccctga agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta
     9481 atgactggga ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc
     9541 tatcggcata ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt
     9601 tttcccaacc aggagaaatt tcattatcag tacttattaa atctattaaa aaccaacccg
     9661 ttctctgagt gttgattcta taatattttt aatttcctat ataaatcaaa aatattatag
     9721 aatcaataag ctaattaaga aacaaacttt agaaactcta gaagaacttc tgaatttata
     9781 tagaagtgtg tagctactta ctaaatatac aaaagttaaa ataaaatact ctggatactc
     9841 tttatactgc attaaagtta gtaatattaa cagcaaagat atcgaaaaaa caataacata
     9901 atatgtgtca aaatttttca taataagaac tcccttactg ttgcatacta acataatcca
     9961 aaaactcttc tacttcagct aaagacattc cactttcttt agtccaaaaa acagtttttt
    10021 ctatcgatcc ttctctgtaa actgtaaatg tcggagtcga gttaattgag tagcgactct
    10081 tgaattcttt ccactcattt tcatcttctc taacttttgc tacattcacg taataaatat
    10141 tttcttttcc cttttctacc agcatatcga atagcttcct gcttttgatg atcgatttct
    10201 tgtgctacat ctagtaaatt ttcatcaatt tctatatcat aaagtatttc actagtgtct
    10261 acatccacaa tatatacttt ttatcagtag caatttttga gtaatccgtt aaaatgatct
    10321 cagtctcgtc actttcatca gtataaatat acgttaacaa tgaactcacc ttttcaatcg
    10381 gtgttagctc taagcctact gtatttttta ctattgagtc attatttacc accataactt
    10441 cgtactgtat ataccatcct atttggtttc catcaatatc agtaattggc tcactagcta
    10501 atggagtctc cggaacaata ttttcctgat atttcttaaa ctcttctgta gcacgaaaaa
    10561 ggttatcatg ttcctcactt gaaactggtt gagtcataga aaatgttact tggctaaaca
    10621 gagggaaaat aaacactaca atcaacaaag tataaaaaaa agcccttctt tttcattttt
    10681 tatcttccaa tcacaaattg aaaaacttat tcctgcaact ttgagtctag tatcacaatc
    10741 gactgactac aactattcat ttacattcta ctttaagttc ctccttaacc attaatttta
    10801 ttaaagatac ttgtaatagt gattctcaca aaatttcatg aaaaatactt tattactgta
    10861 tctaaaattc caatacacaa cttatcgact attatctgaa aaccaaaact tattattcct
    10921 tgatgttatt ctagcaatag taactgcttt tttatttttt tgcatatttt ttcggcgtta
    10981 ctttttaaag ttaacttttt cgacaaatag aaagagatac gaatcactga tttttttgaa
    11041 gaaaagaaat ggagtgagct gaacgttaga gatattggga gctttcgtaa ctgcttatta
    11101 aacgataatg gttatttgca accgtattgc tcgcttctac atgaaatatt tcatgaaata
    11161 tttcgcaaaa cctttgatta tgtagttacg tttccattct tgactaaaaa tgtttcaaaa
    11221 acgaacaaag gtgttaaaag gtaaagtgat caatactact ggtcaaaata agaggttgaa
    11281 agggaattga aaaataacta cagataatac ttttataaac gtacgtgctt tgtggttctg
    11341 tagttaacta tataactgga attagggaat ggtatcaatt tgaaaagaaa gattctacca
    11401 atttatcata tgttaaaaat ggaaaatcaa cagcagttct ttcggaaacc ttacacaaaa
    11461 accgattatt gaaaatagcc gaaaaacgtt tttcggctat ttttttaatt aagtttcctt
    11521 cttcttacct tttcccacta ttccgtttca catctttgat gatcttcagc gcacgttttc
    11581 gtgttttgat atttgggctt ttcaagcggc ggtatgcact tgagatatct gttttttcca
    11641 tatggacgac ccactttctt ctttgattgt agtcatgcta aaaaacacaa atcgaaacaa
    11701 ttacgatttg tgttttaaat ggtatcaaaa gttaaaatga aagtcaactt gttaaaacag
    11761 tcgatcagac tagctgattg actcctatct ctggaaataa attttcatta ttacgtttta
    11821 gctacctaaa aaaggccctc ttgtccgtta actttaatat ctattgttat agctagttta
    11881 cctattgaac gtactgtgtg ttcgatagaa cactaaaaaa gaggctggga cagaagcgtt
    11941 taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttca
    12001 gcttatttcc gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac
    12061 aaaagtgttc tttacttttg tctcacgctc ttttaaacta tttcgaagta ctaaacttat
    12121 gaagtaagaa tacctaagcc agagccaatg tgctcgcctg caagcaattg ctcttttgct
    12181 tgtcgctgta aactcgtact ttctggataa tcatgtattt gggcatctac tcgtccaaag
    12241 atccaaggga ctccactatc gtcctgttca tcaactgata ataatcgata atcggctgca
    12301 aaaagacaga tttttgactc ttttttcaac catttagtaa gattgggtga tagtaaccaa
    12361 gtatctgtgt acatcacttg gtaatggtaa tcaggaaaat aaagttcaaa aaaacgaatg
    12421 gcactttcgt aactatctct tatcttattc tgagataaat ctgcatcgga aggaatatga
    12481 agagaaaggc actgctttcc ttctaatctt agatccgtgt accctttcgg aaaatatgtc
    12541 atttcgtact ccaattcacc aatccgaaag atcaagccac tagtgtaatg ccaaatccaa
    12601 aaaccacggt caaagcgata gcttcctgtt acttggaaat gttcatgaat aaatctagta
    12661 aaagcattca ttgttgccaa atagatagtt cttggaatct tcaatggttg ataaattttt
    12721 ttccaacttt gagaagctgc atatagataa atagctaatg ctgcaaaagg ttcctcttca
    12781 tagactttgt ctattttctt tgcagtcgcc tttgctttat taggatccat tagttcatga
    12841 ctatattgct caaacacctc atcaaggatg ttttttgctt gtacaatcat ttgctctata
    12901 acgactttgg gaagagttaa agtttctgcc agaatttgga tttcattgct tattttcttc
    12961 atcaaatttc cccttttcat atctttgaac ctcattgttt aaatgactga ttatccggac
    13021 accatatggt ataaacatac ttttattgta cactaactat aaaacttaaa ctttctatga
    13081 agaaagaata tctgtaagac tttcaaggaa agcattgacc atctcaggaa agtttattca
    13141 gttaccatca taaacaaaat tttttgatag aata
//
LOCUS       DACCIQ010000065        13017 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCIQ010000065 DACCIQ010000000
VERSION     DACCIQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.067"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..693
                     /locus_tag="IVM62_001973"
     CDS             <1..693
                     /locus_tag="IVM62_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845936.1"
                     /translation="LFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTK
                     NNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESI
                     YHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASIN
                     GKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSK
                     QKQPRREVQHSH"
     gene            complement(838..1578)
                     /gene="truA"
                     /locus_tag="IVM62_001974"
     CDS             complement(838..1578)
                     /gene="truA"
                     /locus_tag="IVM62_001974"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6845937.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1769..2404)
                     /locus_tag="IVM62_001975"
     CDS             complement(1769..2404)
                     /locus_tag="IVM62_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6845938.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2551..3792)
                     /gene="sstT"
                     /locus_tag="IVM62_001976"
     CDS             complement(2551..3792)
                     /gene="sstT"
                     /locus_tag="IVM62_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP6845939.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3990..4988
                     /locus_tag="IVM62_001977"
     CDS             3990..4988
                     /locus_tag="IVM62_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845940.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5001..7019
                     /locus_tag="IVM62_001978"
     CDS             5001..7019
                     /locus_tag="IVM62_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP6845941.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7079..7795)
                     /locus_tag="IVM62_001979"
     CDS             complement(7079..7795)
                     /locus_tag="IVM62_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6845942.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8022..8564)
                     /locus_tag="IVM62_001980"
     CDS             complement(8022..8564)
                     /locus_tag="IVM62_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6845943.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8569..8796)
                     /locus_tag="IVM62_001981"
     CDS             complement(8569..8796)
                     /locus_tag="IVM62_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP6845944.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8875..10755)
                     /locus_tag="IVM62_001982"
     CDS             complement(8875..10755)
                     /locus_tag="IVM62_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6845945.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10944..11558)
                     /locus_tag="IVM62_001983"
     CDS             complement(10944..11558)
                     /locus_tag="IVM62_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6845946.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11700..12260
                     /locus_tag="IVM62_001984"
     CDS             11700..12260
                     /locus_tag="IVM62_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6845947.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12565..12879)
                     /locus_tag="IVM62_001985"
                     /pseudo
     CDS             complement(12565..12879)
                     /locus_tag="IVM62_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4082 a         2569 c         2057 g         4309 t
ORIGIN      
        1 ttatttcctt ggcaactttt taaccataca ccagttaaga gtctacaatt tgtctggcgg
       61 ttaaatggtt tttcttcttt atttatagcc tatacgatga gtatagtgat ctattatatt
      121 ttttctacta aaaataattc atggaaaata atggtcttta cgtttcttgc actaatattg
      181 catctctcag gagtaagcaa ttctatccat gctaacaaag attcattaac acttatagca
      241 cctgaagacg ctgttgctat agctgaaaat tacaatcata ctgattatgc aaacaaagag
      301 tcaatctatc atcctgatat gattaataat gatcaatatt tactaggtaa ccaggaaata
      361 aatccaacaa ctcattttat gagtaacaaa cttacaattg aattagataa ttcgaataat
      421 aaaaatactg tcttgacgac acctatttat cgttataaag ggcaagtggc ttctattaat
      481 ggcaaactag tacaaacaaa attatctaag tttggcacaa ctgaattaac tattcctcca
      541 ggtataaata aagtcgttat tacttatcag tacacaaaac tagcaattgc ttcaagatat
      601 ttatctatcg ttactttgat tttattttta ctttatcgat ttactttttc taaacaaaaa
      661 caaccgagaa gagaagtaca acattctcac tagattttta tttaagaaag actttttagc
      721 tgtacacata aacaggctaa aaagtctttt tttatatttc tttaatttgc ttcatacatt
      781 atttaacatc gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta
      841 tcgataataa acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg
      901 ccgcactttg ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat
      961 ttctagtaat gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa
     1021 agtaaaatga agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt
     1081 ggattttttt gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt
     1141 ttcgcaagct gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt
     1201 tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac
     1261 cgcattatat cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc
     1321 ccgcggaagg taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt
     1381 tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat
     1441 aatttctatc tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt
     1501 tttatcgtca tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt
     1561 caacttgatg tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac
     1621 ctttttaaga ttgtagcaca aaatatagct ccttgcttct ctaagacaga agctatacaa
     1681 gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg
     1741 ctgactgttc aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaaccagg
     1801 caatgggcac ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta
     1861 atggccaaga atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac
     1921 tgatcacaga cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga
     1981 tcaagaaaag aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat
     2041 tatggaccca tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg
     2101 taaagataca atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca
     2161 cacgagatgt actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc
     2221 ctgaaaccac agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata
     2281 cactgatcac gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag
     2341 tcaaaaaatc actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat
     2401 ccatgatacg cctccaataa caattaccta tgcttttaat tgttattata aacgacttta
     2461 tcttgattta acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga
     2521 atcattttaa aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc
     2581 tttcaatttg attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa
     2641 caacagatca cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat
     2701 aaatccaata ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc
     2761 taatggaatc aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc
     2821 agacaaaata cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac
     2881 agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc
     2941 taacgggatc gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat
     3001 gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt
     3061 caaacaaaag aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg
     3121 gtacacaact aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata
     3181 ctctgccaat ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc
     3241 tatcggagca aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc
     3301 caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca
     3361 gaataagacg ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt
     3421 cgtcaacata gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc
     3481 ttgtaaagta ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag
     3541 caatgttccc actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt
     3601 atgcttggca attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa
     3661 gcaactgaca aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc
     3721 aaacaatatt ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa
     3781 cttctctaac aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa
     3841 agaaactaaa ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa
     3901 ttttgtactt tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta
     3961 atgggcaact tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt
     4021 tattcttgaa aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta
     4081 taatgatggt tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa
     4141 accctggtcg attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt
     4201 tgatcccact ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt
     4261 atttaagtcg ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt
     4321 acgtacaagc tggcagcttg aatattcgtg attattggct tatcctaaca cctatcagtc
     4381 agaattactt tacttatgct gtttcttgta ctttgctttt ttgtagtatt ccttatacag
     4441 ttggattttt aaataaatta tcgaaaacaa caattaaata tctactatta tttttaacaa
     4501 ttggattagt tggctgctct actctattta ataaagaccc gtttgcatta cagaatggac
     4561 agagtatttt atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact
     4621 ggaaagaaaa ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg
     4681 gtttgattat tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc
     4741 tgaggttttc taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat
     4801 ttttagaaat gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat
     4861 ttttgattgt cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat
     4921 ttatgaaacg tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt
     4981 ttatttgata tttattagca gtgtccctcc tagttatctt tttattttat atacaaaaaa
     5041 tccgtttcat tcatcgtttc atcgaatact tcgcttttga ttcgattgaa caattaactc
     5101 agcgttggaa aaaaagaatt cattggcttt ttgttcataa aagttatttt ttagttgcag
     5161 cattttttta ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa gaaggttgga
     5221 aagaaactat ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc atgatcctga
     5281 caatcatcat tattatggga tttttcttct tactattgtt gatttccaat cgtttttggt
     5341 acgccttgac tgctaccttg atcattaact tactgctcac tatttcgaca gtaatcaaaa
     5401 tggaaatgcg ggaagagcct gtctttcctt cagatttaaa aatgctgacc ggattgtcag
     5461 aactattatc gatggttagc ccagtcttat taatcgtggg cggattgatc cttctcctat
     5521 tattgattac tagtattatt gtacaacgta gactacaaaa acagtattcc ttgaagattc
     5581 attggaaacg tcgatttatc agtatcgctg tattattagg atgtttttcg ggtgtctttt
     5641 tcattaatca caaaaattcg ccttctttct tgctgtttaa tttatttaaa gtaaacaaga
     5701 ctttctttaa ccagcaaggc gccgtaaaag ataacggacc agtcattcaa ttcctaaaca
     5761 atatcgacat taaaatcatg gaagaacctg ctggctattc gaaagaaaaa attaatggaa
     5821 taatgaaaaa gtacaacaca aaggcagatg aaatcaacca aacaagaagt gactggttaa
     5881 acaatcagac gatcgttctc aatttaagtg aaagcttcag tgatcctgca cgtgtcccta
     5941 atctgacagt acctacagac cctattcctt acatcaaaag cattataaca gaaggaacag
     6001 gaggattgat gttatccgtc ggttatggtg gcggaacagc taacatggaa tgggaaggac
     6061 tgacaggctt gagtatcagt aacctgtctg cttcgttagt tacaccttat acccagctag
     6121 tggagcgtca gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa atagccatcc
     6181 atccttttac agctgctctt tatcgcagaa aacaagtgtt tgataaattt ggttttgaca
     6241 ggttctatta tttagacagt ccggataaat taacttatac ggataaaata gaacaaagcc
     6301 cacgcatatc ggatgattca gcatacaaag aaaccttgaa agctttacaa agcaatacgg
     6361 aaacgtcaca attcatccaa ttatccacta tgcaaaacca tatgccttat gatgattact
     6421 atagtgaatt gaactacact gctgaaggaa ctgctgtcat tcctaataga agaaaagaat
     6481 tacaaactta tatgcaaggc cttcactata ccgatacagc agtaaaaaaa ttcatccaag
     6541 aaatcgacaa aatcgataaa ccgattacct ttgtttttta tggtgaccat ttacccgcaa
     6601 tctatagtgg gaatgattcg aaaaaatatg gattagaaca gcaccagaca gattatttca
     6661 tttataacaa caagtatagt cgtgaacaag cagctacgtt aaataaagaa gttgttgctc
     6721 ctaataactt tggtgctatg gctcttgagc atgcgaatat caaagtaact ccttactatg
     6781 cgttgttgac agaagtatct aatcatctac ctgcattaac aattgatcct acagaaagct
     6841 tgtctaatcg ttttaatgga aaacaggtct ttgttacaga ggatagtcaa attatccaag
     6901 aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg atacaatatg
     6961 atcttgttgc aggtaaccag tacagtgcaa catgggcgac acaaaaaata aaagactaac
     7021 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
     7081 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
     7141 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
     7201 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg
     7261 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
     7321 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
     7381 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
     7441 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
     7501 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
     7561 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
     7621 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
     7681 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
     7741 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
     7801 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
     7861 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
     7921 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
     7981 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     8041 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     8101 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     8161 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     8221 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     8281 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     8341 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     8401 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     8461 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     8521 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     8581 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     8641 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     8701 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     8761 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     8821 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     8881 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     8941 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     9001 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     9061 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
     9121 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
     9181 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
     9241 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
     9301 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
     9361 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
     9421 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
     9481 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
     9541 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
     9601 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
     9661 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
     9721 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
     9781 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
     9841 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
     9901 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
     9961 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
    10021 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
    10081 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
    10141 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
    10201 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
    10261 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
    10321 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
    10381 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
    10441 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
    10501 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
    10561 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
    10621 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
    10681 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
    10741 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
    10801 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
    10861 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
    10921 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
    10981 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
    11041 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
    11101 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
    11161 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
    11221 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
    11281 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
    11341 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
    11401 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
    11461 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
    11521 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
    11581 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
    11641 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
    11701 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
    11761 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
    11821 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
    11881 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
    11941 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
    12001 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
    12061 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
    12121 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
    12181 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
    12241 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
    12301 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
    12361 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
    12421 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
    12481 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
    12541 tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc
    12601 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
    12661 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
    12721 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
    12781 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
    12841 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
    12901 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
    12961 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaa
//
LOCUS       DACCIQ010000066        12868 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCIQ010000066 DACCIQ010000000
VERSION     DACCIQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.068"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..195)
                     /locus_tag="IVM62_001986"
     CDS             complement(<1..195)
                     /locus_tag="IVM62_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845948.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="IVM62_001987"
     CDS             complement(211..825)
                     /locus_tag="IVM62_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6845949.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="IVM62_001988"
     CDS             complement(972..1655)
                     /locus_tag="IVM62_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6845950.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="IVM62_001989"
     CDS             complement(1764..2222)
                     /locus_tag="IVM62_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6845951.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="IVM62_001990"
     CDS             complement(2306..4069)
                     /locus_tag="IVM62_001990"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6845952.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="IVM62_001991"
     CDS             complement(4290..5750)
                     /locus_tag="IVM62_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845953.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="IVM62_001992"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="IVM62_001992"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6845954.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..7956)
                     /locus_tag="IVM62_001993"
                     /pseudo
     CDS             complement(6907..7956)
                     /locus_tag="IVM62_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            8049..9008
                     /locus_tag="IVM62_001994"
     CDS             8049..9008
                     /locus_tag="IVM62_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6845955.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(9017..9400)
                     /locus_tag="IVM62_001995"
                     /pseudo
     CDS             complement(9017..9400)
                     /locus_tag="IVM62_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(9431..10645)
                     /locus_tag="IVM62_001996"
     CDS             complement(9431..10645)
                     /locus_tag="IVM62_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6845956.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(10731..10958)
                     /locus_tag="IVM62_001997"
     CDS             complement(10731..10958)
                     /locus_tag="IVM62_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845957.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(10986..11228)
                     /locus_tag="IVM62_001998"
     CDS             complement(10986..11228)
                     /locus_tag="IVM62_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6845958.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11260..11817)
                     /locus_tag="IVM62_001999"
     CDS             complement(11260..11817)
                     /locus_tag="IVM62_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6845959.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(11830..12018)
                     /locus_tag="IVM62_002000"
     CDS             complement(11830..12018)
                     /locus_tag="IVM62_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6845960.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12031..12597)
                     /gene="amaP"
                     /locus_tag="IVM62_002001"
     CDS             complement(12031..12597)
                     /gene="amaP"
                     /locus_tag="IVM62_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6845961.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3848 a         2638 c         2132 g         4250 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatcgccga ttgtaaaatt aagctagaca
     7981 actaaaaaaa gtcatttgtg ctacactcaa aatagttccg acgaaagaat tataaggagt
     8041 gagtacaaat gacctataac catcttacac caactgaact cgtaatgata gaagcttatt
     8101 tcaatcaatc acaacctgtt tcaaaagtag ccaatctgtt acaacgttct cgacaaacta
     8161 tttataaggt ctatcgtttt cttaaatctg gtggaagcgc aatgagctac tataaacaat
     8221 ataagaaaaa caaacgtaac tgcggaagac atccgattgt tttacctgat gagcaacagg
     8281 aatacattca aaaaaaggtt gttcaagggt gggctcctga tgtccttatt ggtcgagcag
     8341 agtttcctat tgcttgttcg gtacgcacac tgtatcgaat gtttaaagat aaaacctttg
     8401 acactcacga tttaccaatg aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag
     8461 gaaaacaagg gtttcgccgg tcaatccgag acagaaagac agactatacc cagtttgatc
     8521 atgaatttgg tcacctggaa ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca
     8581 tcacattagt tgaacggcta tctaaagtca tcattacctt gaaacccgaa ggcagacagg
     8641 ctaaagacat tgaaaatagg ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta
     8701 aatccatcac atttgattgt ggaaaagaat tctcaaattg gaaaaagatt agtaatacca
     8761 acgatatttc aatttatttt gcggatccag gaacaccttc tcaacgagga ttgaatgaac
     8821 attcaaatgg cttattacgt aaagatggtt tgtataaagc tatggatttt aatggtgtat
     8881 ctgaaacctt tatccaatcg gtggcatcga aacgaaatca tattcctaga aaatcattac
     8941 actatcggac accaatggaa gtatttttga gttacgtcac aaaagatgat ttgtctaact
     9001 taatttgaca aacgaaatat ttattgtttt gtatagcacc aatacccgtt gcataggata
     9061 atgcaccagt taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga
     9121 aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac
     9181 cgcctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     9241 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     9301 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg
     9361 ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt
     9421 tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt
     9481 cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca
     9541 gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc
     9601 agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac
     9661 ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct
     9721 ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg
     9781 cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc
     9841 ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag
     9901 taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat
     9961 ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac
    10021 cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt
    10081 gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc
    10141 caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc
    10201 aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat
    10261 tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt
    10321 ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg
    10381 taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc
    10441 caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa
    10501 aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag
    10561 ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc
    10621 ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa
    10681 agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga
    10741 cagacaatta ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga
    10801 caaaaaataa acggtcttct ttttatagaa aaccgtttat tttcgtcttt ttttattttt
    10861 aaagatattt tttcataagg aataaagaat acttcatata aaatatataa aaaagaaccg
    10921 cagcgagtgt cgctacggtt ctcaaatttg ttcttcattt caacataagt ttttcttcaa
    10981 tttgcttata atttacgtgc aaagaatgaa acgactaata ctaaaatgat tgcaccgatc
    11041 aatgatggaa tgatagccat tcctgcaatt gttggtcccc aagttcctag taaagcttgt
    11101 ccaattgctg aacctactaa accagcaatg atgtttgcga tgatccccat tgatgatcct
    11161 ttattcgtga ttgctcctgc aattgctcca atgataccgc cgacgattaa tgcccaaata
    11221 aatcccattt taaattcctc ctaactgact tcttacttct tattgtacac gtggttcgtt
    11281 attttctttg actttttgtg cacctttgcc tacagcagat tttgctttat ctgtttggcg
    11341 tgaagcaaat tgtcctgtgg attttgctgc ttctgttact ttgtcttgta ctgtttcttg
    11401 atctttttgg tattcttctt gtgttttgat gtctgcaaca ttcacattca cttcgataac
    11461 atctaaatgt gtcatattgt ttacttcagt actgatcaat tctttgattt ggttgtaaat
    11521 atcttcgatg tctttaccgt attctacgat cacatctaga tccacagcta cttgtttctt
    11581 acctacttct gtattgatac ctgctgttac gttatctgtg ttgactaatt tttcagccat
    11641 atttgagaaa aatccgccat ctaatgtcaa tagaccatct acatctttca atgcataacc
    11701 gatgattttt tggatgactt tgtcttcata agtcaattca ccattcatca tgcggttttc
    11761 tcctttttca tttgccaaaa ctgatccatt tgttgtattc atgtttttgt tttccatctt
    11821 tgtttcctcc tatgatttaa aaaattgatc aactattcct gactgtttca aataaaaacc
    11881 gattcctgcg ccaattgctg tcatgattat gatcaacaat gtcttgaaaa atccaatcgt
    11941 taaccataaa acagctagca gtagtccaag acccccaaag ataataggga gcttgtatgc
    12001 tgaaaatagt tctttcatta tttggccctc ctattccact cgagagtgct tgtagtctct
    12061 tgtatcttct tgttcttcat aaccgctgta tgtgactgcc actttgaccg tttcttttgt
    12121 tccgagtact ttagttactt cttcttcgat atcttgcatc aatacacctg ttttccctac
    12181 taatgaagaa gttcgtttca agtctccgtt gacatggata tggattcgat ttttcgttga
    12241 acgtatccgt actttaggtg aatgaatgaa ctcttcttcg tgtaaatggg aacgtaccaa
    12301 cccttcgatc gcttttttgt cgattgttag tttgccatct tcccgtttca taacgaaagt
    12361 tcctcttgac tttggataga aaagaacgac tagcaagatg ataatagcaa gaacggcaaa
    12421 tactacagct gcccagaaaa tatattgttg catgtagtaa ttcgttaaag taaagaagcg
    12481 aaatcgttcc aattgttcag gcataatata cggcgcattg atcaacgcaa tcgctagaaa
    12541 tactgaaagt agcagtaaac tcatgataac accgattatt tttaacccct tattcatttt
    12601 ccacctccta taaacttatt tttaccctgt aaataactct accgacaatc acagtataga
    12661 tagcgaacag ttagcatagc atcagtaaat aaagcgctac caaacattgc tggatcataa
    12721 cggcgtttta ttgccagtct gatactgaaa taacaattgc ttacttattc tctatttact
    12781 tttgattgct ggctatactt aaaggatatt attcaagatt aaaaattgca aagattatgc
    12841 tcagttatta ttacaagatt ctatttat
//
LOCUS       DACCIQ010000067        12859 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCIQ010000067 DACCIQ010000000
VERSION     DACCIQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.069"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(440..1003)
                     /locus_tag="IVM62_002002"
     CDS             complement(440..1003)
                     /locus_tag="IVM62_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6845962.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1018..2061)
                     /locus_tag="IVM62_002003"
     CDS             complement(1018..2061)
                     /locus_tag="IVM62_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845963.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2194..2598)
                     /locus_tag="IVM62_002004"
     CDS             complement(2194..2598)
                     /locus_tag="IVM62_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6845964.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2831..3466)
                     /locus_tag="IVM62_002005"
     CDS             complement(2831..3466)
                     /locus_tag="IVM62_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6845965.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3732..4784)
                     /locus_tag="IVM62_002006"
     CDS             complement(3732..4784)
                     /locus_tag="IVM62_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6845966.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4797..5735)
                     /locus_tag="IVM62_002007"
     CDS             complement(4797..5735)
                     /locus_tag="IVM62_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6845967.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(6186..6836)
                     /locus_tag="IVM62_002008"
     CDS             complement(6186..6836)
                     /locus_tag="IVM62_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845968.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6936..7445)
                     /locus_tag="IVM62_002009"
     CDS             complement(6936..7445)
                     /locus_tag="IVM62_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP6845969.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(7646..8515)
                     /locus_tag="IVM62_002010"
     CDS             complement(7646..8515)
                     /locus_tag="IVM62_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6845970.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(8535..9656)
                     /locus_tag="IVM62_002011"
     CDS             complement(8535..9656)
                     /locus_tag="IVM62_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP6845971.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(9658..10053)
                     /locus_tag="IVM62_002012"
     CDS             complement(9658..10053)
                     /locus_tag="IVM62_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6845972.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(10072..10869)
                     /locus_tag="IVM62_002013"
     CDS             complement(10072..10869)
                     /locus_tag="IVM62_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6845973.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(10871..11659)
                     /locus_tag="IVM62_002014"
     CDS             complement(10871..11659)
                     /locus_tag="IVM62_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6845974.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(11673..12149)
                     /locus_tag="IVM62_002015"
     CDS             complement(11673..12149)
                     /locus_tag="IVM62_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6845975.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(12221..>12859)
                     /locus_tag="IVM62_002016"
     CDS             complement(12221..>12859)
                     /locus_tag="IVM62_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6845976.1"
                     /translation="LGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKEN
                     NILFIVGTLNPHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFS
                     LTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQN
                     TLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         4030 a         2714 c         1925 g         4190 t
ORIGIN      
        1 aggtaatggt ctgaacacca agaattgttc caacatcgaa ttgcttaact aatgagttca
       61 aagcttaatt atgtggctct tatatttacc gagcgtggat tgcgatctct ttagcgaaac
      121 aaacatcttt cataaaaaaa acagccatat ccgctttctt ggatatgact atttactact
      181 actttactat ctttcattct tcttgtctca cttatgataa ggcgctgcgt atcatgagtg
      241 aatgatatta ggaaaacttc ataaaataaa tcttttatac atttccactt ctagatggtc
      301 attcatgtta gaagaatcaa actatttttt tactgtcgta ataatattaa aacactagaa
      361 caaatcaatt aaattccata aaatataaat aatagaccta gcatcatctg gtataaaatg
      421 gatgctaggt ccgttatatt tactcaaaat tgattctacg aatagatctt cttatcattt
      481 ctattttagt agtcatgcgt tgttgttctt ctaaaggtgg taatggtata agtagtttag
      541 ataaatttgt accatttaca tttggttgcc cagttccgta agaaattttt tctaataact
      601 tccaatataa tggcgagtct aagaagcagt ctacgtattc tgataataat ttctcaacta
      661 acctaattcg aatcaagtaa gaagcaaaaa cagattcctc acttatttcc ttaaccaaga
      721 aactttttcc catagttcct cctgtccttg ctatcaaaat gtcattttct tccaatctta
      781 aatcaactag tttagaatta ctaatatcac agtaaggcac actggaccaa tttacacgtc
      841 cctcttgtat atctgtgatt ctcaaaaact taacattgcc ttgttttttc gccgaatctg
      901 tatagccata ttgaatctta gtagaaatac ttcctaaata aacccatagt cgaacatcgc
      961 tagatgattt ttcttgtcgt tctttatgaa caacagtgat catcttgagt tgtccgacta
     1021 ttttttagtt gcagcaatct ttttgggacg cttatttgca tgaagcaacg ttgctaagtc
     1081 tagtaataaa acattggcta acacaggatt ggctttctcg cgatacgctg cggcgccact
     1141 ttcaaacaat actgctagac gatcttctac aacaacgatt tgttccaaat acggtggaaa
     1201 tttgattcgt tttaggatcc ggctaggaga gtcccgctcg gacactaaga gttccgaggt
     1261 ataaggacca taagaacgac tggctaagag ataatggcca taaaaagcca caccttgaat
     1321 cttatcagat gtttttctgc tcattgtcac ggttggttgg attttgtctt tgcggcgaat
     1381 tgctgttttc atttcagcta tcgtcggcaa tagatagaaa gtcaactgtc cttcaccttt
     1441 tagtgtgaag ttgccagata ccagttgatc attgttttga gtcaaatacg atgcttgtgg
     1501 aatctctgag agatcagttg tcagccaata agcgattggc tgtcccgtct cttcagatgt
     1561 atctgctaag atatcgatca aacgtaaagc ggctactcgt ccagtagcag atcctgtgat
     1621 tgtcaaccat aaaagttccc gttttgtatc atatactaag ccccctacat gtggccgttt
     1681 aggtaaaatc agcagtttga tcggctgtcc tgtttctcta tccagtacat gaataataga
     1741 atgatgctga tggtcatagc agtaggcaga aataaacaca aaatcttgag aaaaagccaa
     1801 cccttgcggc gtcatccatt gacaaacctc attttcgccg gtttccggca ccaccattct
     1861 tgtttctgtt aatccaggaa tgttatattg ttttgcttca gccttttccc aagctgcttg
     1921 aacaatagga taccgtttta actttttttc aatatcctga tgtatccgtt gctgcttcca
     1981 atgcttaaac tttaggacat agtaaacaag aaggccaatg accagcaaca ctaaaatcat
     2041 tattgctacc cgctgtgtca tttttttgcc tccttgttct attcgatcaa tcgtccatat
     2101 accgctatta attaccgtaa ctacatgttt cttttactag cgtttcttta taggatcttt
     2161 cataatctca tttatagtaa cattaagaat cccttattct actatagtca cttccacatc
     2221 aatattgcct cttgttgctt tagagtatgg gcaaaatgcg tgggcttctt ctgcgatttt
     2281 ctctgcttgg tccttctcaa gtcctggcaa agaaacttct aaaacgaccc cgatttggta
     2341 gcccccatct tctaattgaa ataaagatac ccgtgctttg acttcagaat cagactcaag
     2401 attattttct tccagcatgt gttgaaccgc gccgttgaag caagatgcgt atccagccgc
     2461 aaaaagttgt tccggatttg tcccttcact atggattcct gggcggtcga tcttcatgtg
     2521 catacttccg tttggtgcct caacttcgcc atcacggcct ccacgattga tcattgctgt
     2581 ttcgtacatt tttttcattg agatcatcct ttctttcgtc gttctatatt aagtgtaacg
     2641 agtatttttt aatctgacaa aggtaatgct tgcattcggc gacatcagtt agacaacatc
     2701 gatgatcagt gatagaagca gtttatctat acaaaaaaag aagggttcta tgatatttgg
     2761 tgtccttact cgccttgagt aacaacacca aatatcatag aaccacttaa tcagcttcac
     2821 actgcttttt ctactttcgt agttgaaaaa aacttaaaca aatcatcggc aaatagttaa
     2881 agaaaaaact taatcgttga aagaagccca attgcttaaa gaaggcaaac tgactaatcg
     2941 acggaatccg tgctaatccg taaagacctg caattagtaa actaataact gttaaaacaa
     3001 gataaaaatg gctaagcgta ccttgaccac tttgacgata aagcagaaca agtaacaatg
     3061 ggaaaagtag aaagcctgca tagcctagcc cagaacctat attatggatc caagtcgata
     3121 gattacaaga gctttcattc gtattgatac tgaataagcc agtaaaaata caatcaccta
     3181 tcccatataa accaactgtt gcaacaacaa gtattgcaag tgtttttgat gttccaacaa
     3241 ataggtgata caacgcaggc aacgaaagaa caaaaaaaag tccagataca actgaccaga
     3301 ccaagaaagc acgtctgact ggactatcta cgtcaccaaa cacactaatt acggtagtca
     3361 tttgattcag tttgggataa aaaaggccaa ggatatacgg tgtcgataaa tcactcatca
     3421 cacctaataa taaaaaataa aaaccatatt tttgcaaaaa aatcattttc ttcccctact
     3481 ttttagtgat tattcgatac atccaacaat caaatttttt ctcattttaa cacatatttt
     3541 tattctagtt gtcttttgaa caatcaataa cataaaggtg agtttcttct acattttaaa
     3601 aaatcgaatg gcactgaaga gttcagttgt cagcatcaat ctcctactca aaaaggcaaa
     3661 aaaacgtaag tcagatgttg ttgacatctc acttacgcat ccattgattg aaaatttctt
     3721 agtaaaatct actagcctaa aaatttgaag acttcttctc gatcatacag tttaaatact
     3781 ttctcgcata aaaattgagt aattagtgcc gcaaaaacag ggatgactaa ccaacaaaga
     3841 acaacaagaa agccatttag ccctgcatct aaagaagcca acggaccaac aagtccgacc
     3901 acgccgaaac cagcagaagc aggcgtacct gaaatagaaa atagagcgac aggaatagct
     3961 gtaatcactg cactaactaa acaaggaata agaatcacag gttttctaaa tagattaggc
     4021 atcatcatct tcatagcgcc taaagcaatc gcaattgtca cacctgactt attaaccttc
     4081 caagagttca ctactaaaac aaaagtggtt gcagctacgc ccatcgcagc cgcaccagca
     4141 gaaacaccat ctagttggat tgccattccg attgctactg ttgaaatagg agagataatc
     4201 aaaaaggcaa aggaacaacc aatcaagata gacattaaca gcggttgtaa ggtagtgaag
     4261 ttattaatca atgatccaat tgcagaagta attgctgtta caaaagggaa taaataaaaa
     4321 ccaagtaaac ctgcacctac acctacaaca atcggtgtta acacaatcga cactgaccca
     4381 aaactatttc caatcagtaa aatcagcccg acagcaatcg atgctgtcag catcgtatta
     4441 ataatatcgc ctgtaccagc accaacgtaa acacccgttt cacccaaatc aggtgtgaat
     4501 ttgactacac cagaaccaac aaaagaagct ccggctacaa ccatcattct catcggatca
     4561 aatccaaatt gtaaggcaat caaaccacca atgatcaaag gtgttgctag ttgaaaaata
     4621 actgctaact gtgcgatcgt taccgcaaag ggatacgcac taaaatattt taaaatggca
     4681 cctaatactg cattaggaat caatccaata atgattcctg ttgctgtacc agcgaggact
     4741 ttattcagaa aaattttcgg tgatattttt tgtgaagttt ccattcaatc tctcctttat
     4801 tttacgttca aactatcttc ttttgcatgg acaagctcag tcaagaaatc acaataaggc
     4861 gtcgcaacac catatttctt cccttttcta gaaattgcgc cattgatata atcgatttct
     4921 gttaatcgat ggttcttaat caagtcttga tacattgaag gataatgtaa cccaattgtt
     4981 tccgggtcaa aacaagattc acaatgtgca atgacttctg ggacatctag ttcaatcttc
     5041 tctactgctg ctactttggc aaactcattg acaatcgtcg ccaccatttt atgagcagtc
     5101 gatgtttttc ctagctcagc catattaacg tctaagattg tgcatagtcc gttcattgtt
     5161 ccgttcacac atgctttgcg ataaatagaa tagtgaatat tgtcagaaaa atgagcattt
     5221 aatcctgatt cagacaattt gtctgctaat ttttttgcag ctgcttcttt tccatcacct
     5281 aaattttgta attctactga accacttcca aataatttga cctgaccagg accttcaaga
     5341 cctgccgtcc acatggtatt tccaatatag atattttcca ttggtacaaa tttctcaata
     5401 atatcttcat gcccgatacc atttaataga cataagacct ctgtatcttt tttgattaat
     5461 gattggatat cctgcagcat tttttccagc tgcatcgctt tggtaaatag aataatcaga
     5521 tcaacttgat cttctttttc tacttcggat tgaagaacga ttggtagttt tgcttctacc
     5581 tctttaccat taaaatttgc ttgtaatcca tgttctttga tttgttggac atgttctgcc
     5641 cagccatcga ttaataatac ttcgttccca ctttggtgaa gcatcaaccc aaagcgactt
     5701 cccattgcgc cagcgcctgc aattgctatt ttcattgatt cttcctcctt tttctttcta
     5761 ttatactcat ttgagaatct tttcacaacc tcgttttgtt attcttctct tatttatcat
     5821 gtttatatcc accattcaag ttattttatc agcaaatcaa taaaaactgg aatagaatcc
     5881 caatttcatc gtaagagaaa ctagactagc aataagaaaa tagaaacatt catagaaaaa
     5941 atctcactcc tctgattata aaagaggggg cattaacctc gtgtatacta aaaccttcca
     6001 ttcattcgat ggatgatttt ctttttgtga aaaaatattc attgtagtac gatgttgcgc
     6061 aacacagacg gatggtattg cttattattt attaaaatcg aacaatacac ttatttaaaa
     6121 acttctttcc cagattttct tccattttta cagttataac tagtattatc atgaaaaata
     6181 gccttttatt gtctatggga ataataattt aaaattggta cttttttatt aattagttca
     6241 tcgcccaata atctctccag tttcttagca tctcctgact caaccggaat tatccaagcc
     6301 cagtctaaat aattttttaa aatctcatca tattttgcaa atatttcatt gccatattcc
     6361 gtccttaaaa ttatattttc agtgccagac aagtgattta tattgagttg ataaaaaaga
     6421 tctccatcta aaaaaatatc attagaagaa gatttcccca cagtaatgac cattccatca
     6481 gctctatgtg ttacatacaa ataattgtat gacctcattt cacctaatga atcaattatt
     6541 tcttgccctt tagctctaat atattgatct ctattaccta tctcactctt atttaacaaa
     6601 agttttttca tgtcaaattt aatcggctgt tttatttttg taaattcgct tctactacta
     6661 atccaatccg agattaattt ttcaacatgt cccagagtaa actctgattt attcgctaaa
     6721 tacttttttt gttgtattaa agacatcttt ccccgaagct caaaattgag cctacatcga
     6781 ttctctatct cttcaaaaag catcttatct tcatcattct tatattcatc attcataaaa
     6841 ccaatccagc tcctcgatta agataatatt ttaatctcat acaaaaacta gctctcatca
     6901 aaacaaccat tgttttgatt tctcatgaaa tttctttata cttttctccg attataaatt
     6961 cctaacccct tgagaaaatt acgcatatat ttatctccgc aaggcttaaa atttcgttgg
     7021 ccttcttttc tcaaaaatgc cccgatttcg ccttttgatg gatagatttc tgcctcagca
     7081 aaaacgtcta aaatatcatc agtggtcata gacaaggcga tttttaattt ttttagcaac
     7141 acattgttga tatatttgat ttccttgctt ttttgcggcg tgtctactat cggcttatct
     7201 ttttgcggac cacgcgccaa gagaatcagg ccatttagga atgcgtcaaa aacttggttg
     7261 ttgatggttt tttcatacac attattttct gcattatctt tgttcttatc ctgtactttg
     7321 gtcagtaccg cctgagcttc ttctttagtc agtggtacac cgcctagctc aaaagcttta
     7381 atcaaatccg tatctttcaa atcaatcgca taacgcaatc gcaataacct atcattgtta
     7441 ttcatttgtt cctccgaagt atttagtctt tactttctta cgtacttttt gtcttgctaa
     7501 gatttttagg atcaatgata aaaaccgcat cacttatgat aaggcgctgg gtatcatgag
     7561 tgaatgatat tgctgaatac aaatagtccg accacattaa gttgatcgga cttcataagc
     7621 attttattga ctaatacttt tttgcttaag aattgattct aacatctttt gctaaagtta
     7681 agcatcctat agttcctgca ttatctgcta acttaggtgt gacaatgtat tcttcgatat
     7741 ttggcgtatc gacatagccg ttaattagtt ctaaaaaacg tctttgtact ttctttttca
     7801 gatgttcttg ttgcatgaca ccaccaccaa aaattataat atctggtgaa agcatcaggg
     7861 tcgtattaca ggcacattgt gcaatataat ccgcctcgat ttcccagaac ggatcttcta
     7921 tagaaagatc ttgtcccttg gttcccgtac gcatctcgat agcgggtcca gatgccaacc
     7981 cttcgagaca atcatgatga aaaggacaag taccagagaa agtatctcct gaatgtctct
     8041 taactaacgt atgccccatt tctggatggc taaacccttc aacaaaaatc ccatcttgta
     8101 tagctcctcc accaattcca gttccaacag tgtaataaac aacactggat aaaccttttc
     8161 catgacctga aacatactcc ccgtaacaag cagcattcac atcggttgtc caggcaattg
     8221 gaatttcgaa atgcttattc aacgttccaa caaaatcaaa atttttccag gcagtttttg
     8281 gagtagaggt gatatgtcca taggtctttg attcacgatg gatatcaatc ggtccaaatg
     8341 accctactcc aatgctttct aattgttctt tatagtgatt aaagaaatca attaccaaac
     8401 tcattgtttc attcggtgta gttgtaggaa aactcactct ttctaaaacc tttaaatcat
     8461 cattgccaat ggcacaaaca aatttagtcc cacctgcttc tattgagcca tacatttctt
     8521 actccaccgt ccttttatct aggaattcgt tcttccctgt ctaatagttt ttcgaaaaac
     8581 tcatgtggtt ccttttggta ccaataggca acagaagaaa tatcgtcttg acgctcataa
     8641 agcttgatgt catcatttcc tagggtttgg aaagtaacac gtaaattttc tttgaaacta
     8701 atggggtcgg gtaaatgcca gcggtacaac ccatgcatcg gtaaagcatc atcgccaaaa
     8761 ccatgaactg gatttggatt aggttttcca gcactaaaat aatcccgggt gtgatctctt
     8821 ttagattgaa aaggatagcc cataaatagc gtattaaaat tttgagcata tgccctttct
     8881 ccaaagttct ttttatggaa agcccaagcg ccgccaaaat aatcttctgc tcctgtagat
     8941 gttattgtag ggaaatcttc atctccatct atataaaact taaattctcc ttccccccac
     9001 cagtatcttt ccaatgcgca caaagcaaag taggttccta cataataccc agtgcctttg
     9061 attccatcaa taagggtgta atcttttcct tttgtcgtga tgggttcacg gttccattta
     9121 gcatgaaaat acatcgcatc atccggtact tcagtcaatg cataattaaa tgtataaaaa
     9181 atgtgcttaa taaactcagg atgttcattc gttaaagtaa tcttagcttt ttttcgaaat
     9241 ggcatctcaa aatagcaatt catccctgat gttggattca ctacgattgg taaggaattc
     9301 acttcgcatc ttgtcccaaa cccattacaa aaaaaatctc ctaatggact ttctacagaa
     9361 ggagtggatt catcatccca ataaatacgc aaaattaaat cacgtaagac gaaactccca
     9421 gcgattgttt tgtctgtcac tgtcatccaa aaatgacgta aaattccagg gccttccatt
     9481 tctgctaaag taattgtctc tccagcattt atcggaacag aaggtgaccc ttttctccca
     9541 attcctagat gactcgctgt cattgccgct ttcccttttt caccattttt attttctggt
     9601 gaaatagttc tactttcctc tcccttgtgg agccatatat tatttaaaca attcatttta
     9661 ttcgtcctct atcctttctt cacttttttg attattcata tagatgattt gttgttgtgc
     9721 ctccgcaata attgctccaa tatctaaatt atcatttcct aactgcaaag cgattgacat
     9781 tgccaaggga agattcattc ctgcaattac gtgcactctg tcagaaatat aggtaaaaaa
     9841 cttttgattt acacttcctc cagccatgtc agttaaaaca attatttcgt ctgtagtatc
     9901 cactaactct ttaatttgtt catctatatc atcaatttct tcgacatatg cacataagct
     9961 actaatttga ttatgcatac ctgttaaaaa ctctagtgtc tctttaaaac cctcagctag
    10021 tattgaatgg ctagcaatga tcaatcttct cataattcaa atccttttcc tttacgctaa
    10081 tattccaaat gcagccataa ccatcgcaaa aacaataacg agtagaatca aaatggtcat
    10141 actgattttt ttcttactga gtagataata tgctgcagca gtaataatta ctggaattaa
    10201 tgttggtaaa attttgtcca acattggttg gattttcata ctaacttcac caaaagtgaa
    10261 tttaagtggc gttgaaacac tgataactga agcaattaat gatccaacta ctgttagccc
    10321 caatactgaa gcagcttcag ttaaataatt aatcttttca ccgtattcag taacaatctt
    10381 cattccctgc ttataaccaa attcaaagcc tctcattcgg aaaaagaaga ttaccatgat
    10441 taaaatgaac cataaaaaga ttcctacagg attattttct aaagccatat aagccgcaat
    10501 tgatcccatg atggttggga tcatcgtcat caaaagagag tccccaatac cagcaattgg
    10561 acccatcgta ccaactttaa agtcattaat cgcatctttt gcttctaatc cttgcttttc
    10621 ctccattgca acacaagcac ctaaaataat ggctgccagc caaggctgtg tattgtaata
    10681 ctgaaagtga ttatttaaag cttcgatgta ttcatcatca tctttgtata attttcttaa
    10741 aataggtgac aatgcgtaag tcacagatgc accttgctgt gtttgataat taaaggtaca
    10801 aactgacatc aaccaacgat agttcgcttt cctaagatcc tttttcgtta cttgatattt
    10861 ttcgctcatt ttattcatcc tccccaataa acccaacatc agaagctccc gcagaagcag
    10921 tgatttgtgc atctttttca gctctatgat acacattaaa tgctaaagcg aatcctatga
    10981 tcgccgctcc aataataggt accttcaaaa aagcggctaa gaaaaatcca acaagtaaat
    11041 aactaatgta tcgacctgta ggcataaagc gtaacaacat cgcaatacca acagcaggca
    11101 acatttttcc ggctaatgtt agcccatcca taaaccaatt aggtaaaaca tccaaaacgt
    11161 aattaacagc agactgtcct aaagtaacgg caataaatac tggaattgct gcagataaac
    11221 caaagaaaat tggccccaac cacaaaacac tattcatttt cttaaattct cgtttattcg
    11281 catattcctg tgatttgcga acaacaaaac catttaagat tttgacaatt acatctagtt
    11341 gcacccctaa catacctaca ggtagtccta ttgctaatcc ggcttctact ccaagctggc
    11401 taccaaatgc cgttgcaata attgcgcacg ttccatagtc cggtacagat gagccaccaa
    11461 aaccggcaac tcctaatgac atcaattgaa ctgtaccgcc aactatcaaa cctgttttta
    11521 tatcacccat caaaattcct gtgaataatc cccaaaaaac tgcgtaattt acaaagatca
    11581 tcggaatccc ataataatca acatgtttaa taaatgctaa tatagtcagc ataataattt
    11641 gaaaaatagt aaaatgcatt atccttactc ctttatccta attttgattg aatttcctgt
    11701 gccttatccg ccggaacaaa ttgtgtaaca atttctacac cagaatccaa caatttttta
    11761 tacgtgctga tattttcatc tgatgcatag gctctagaag atagctgtaa tgcattatct
    11821 ttaacaacag taccgccaac aattagtcgg tcaattctaa taccgttatc tactaaatga
    11881 aggattgttt ccggctcttt cacgatgaca aatacttttt cctgatcgta tttccccttt
    11941 ttaaaattcg caatcgctgt ttcttcagtg ataattgata cagccattcc agcaggtcta
    12001 gctaaacgca tacttgattt ttggatgtcg ttatttgcga cagaatcatc gatcaccatc
    12061 actctgtttg ctttagacat tggtgcccat tgcgtgacaa taatcccatg caataaacgg
    12121 ttatcaactc tcgacataac aatactcata aatacttcct cctttatctt tcattttctt
    12181 acaagcaatt accatgccaa aacaaaaaga acttgataaa tcaagctctt tttacataat
    12241 cataaacgta tttaacctca tgaatcggga tgtcgacact ataataatgt tcaacgacac
    12301 tgaaagattg cttaaaatgt gtctgaaatt tttgaaattc ctcactcgtt tcattgaaat
    12361 aaatttcatc ttccaaagta ttttgtttta ctaatcgttc aattaagcaa ctaatgtgaa
    12421 catataatcc gaaacacgta ttattatcaa atgaaattcc tagatcattt tgtaagatgt
    12481 atatagcatc tgaaacccgt tctaataatt tagtcggatt taatatggtc aattgctcca
    12541 ttaaattcgt tagactaaaa ttcttcagaa tattattgtt caaaactttc agttcattat
    12601 cattaaagtg tctattcaat tgattggaga atttacttat attcattcct aaaatcatat
    12661 cttcaattga aaagaattct acatcttcta gatgtggatt tagtgttcca acaataaaaa
    12721 gaatattgtt ctctttacga agctccgata aatagttttc attcactaaa gagtaatagt
    12781 cacacgtttc tatgacaaga tcagtatccc ttggaaagct atcttcaagt atctttttta
    12841 atttaaacga agttcctaa
//
LOCUS       DACCIQ010000068        12634 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCIQ010000068 DACCIQ010000000
VERSION     DACCIQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.070"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(54..1583)
                     /gene="ahpF"
                     /locus_tag="IVM62_002017"
     CDS             complement(54..1583)
                     /gene="ahpF"
                     /locus_tag="IVM62_002017"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP6845977.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1593..2156)
                     /gene="ahpC"
                     /locus_tag="IVM62_002018"
     CDS             complement(1593..2156)
                     /gene="ahpC"
                     /locus_tag="IVM62_002018"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP6845978.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2300..2962)
                     /locus_tag="IVM62_002019"
     CDS             complement(2300..2962)
                     /locus_tag="IVM62_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6845979.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3146..4792
                     /locus_tag="IVM62_002020"
     CDS             3146..4792
                     /locus_tag="IVM62_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6845980.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4783..5865
                     /gene="uxuA"
                     /locus_tag="IVM62_002021"
     CDS             4783..5865
                     /gene="uxuA"
                     /locus_tag="IVM62_002021"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6845981.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5973..7526)
                     /locus_tag="IVM62_002022"
                     /pseudo
     CDS             complement(5973..7526)
                     /locus_tag="IVM62_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            7674..8815
                     /locus_tag="IVM62_002023"
                     /pseudo
     CDS             7674..8815
                     /locus_tag="IVM62_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8941..10020)
                     /locus_tag="IVM62_002024"
     CDS             complement(8941..10020)
                     /locus_tag="IVM62_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6845982.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10123..10563)
                     /locus_tag="IVM62_002025"
     CDS             complement(10123..10563)
                     /locus_tag="IVM62_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6845983.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10620..11030)
                     /locus_tag="IVM62_002026"
     CDS             complement(10620..11030)
                     /locus_tag="IVM62_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6845984.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11210..11644)
                     /locus_tag="IVM62_002027"
     CDS             complement(11210..11644)
                     /locus_tag="IVM62_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6845985.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11744..12592)
                     /locus_tag="IVM62_002028"
     CDS             complement(11744..12592)
                     /locus_tag="IVM62_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6845986.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4222 a         2351 c         2242 g         3819 t
ORIGIN      
        1 attcttgtca ccatcctttt ttccgctctc tgacttttta caatcttcat agctcaagtg
       61 cgcatcagat aatcgaatgc tcctaaagct gcagtagctc ctgaacccat tgaaacaatg
      121 atttgtttgt atggactatc tgtacaatca ccagctgcga aaacgccggg gatattcgtt
      181 gcgccatttt tctcggtcac tatttcattt cttggattca tttcgatcgt tccatctagc
      241 caatccgtat tagggagtaa gccgactaga atgaatactc ctgaaacagc tagtgtatgc
      301 atctccatcg tacggcgatc gatataggag agagattcta cctgattcgt accgctgatt
      361 tctttcgtcg ctgcatgttt gataacagtg acattcgcaa gagagactag tttatcctgt
      421 aagatttgat ctgcttttaa ttctggtaag aattctagaa catatacatg ttttgctaac
      481 ccagccaaat cgattgctgc ttcaacacca gaattaccac caccgataac agcaatctct
      541 tttcctttaa ataaagggcc gtcacagtgt gggcaataag caattccttt atttttaaac
      601 tctttttcgc ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt
      661 tttgctttaa gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt
      721 aaggaaacgg cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct
      781 acttgctgca tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct
      841 agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta
      901 cgtgctgcat agatagctgc actactagct gctggtccac cgccaatcac taatacatca
      961 taaggttctt tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt
     1021 tgttcgatcg tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact
     1081 gccatgattt ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga
     1141 gtaatatttg gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta
     1201 tgacaagtca agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta
     1261 agactcaaag attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa
     1321 aaagaggaaa attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca
     1381 actggattga tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta
     1441 ggagacattt ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta
     1501 tccaaactaa cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt
     1561 tgtttagttt ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt
     1621 ggtttcaatg tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta
     1681 cgtacatatt gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca
     1741 tctgcattga tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc
     1801 tgagctaatc cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga
     1861 tcaccgatca tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg
     1921 tgagtaaagt gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta
     1981 ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga
     2041 tagaagcaaa gaatactcca gttccccata agatcttttt cactgacttc taaaaattct
     2101 ccttgatgat aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta
     2161 aaacctccaa taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc
     2221 aatggatatt ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag
     2281 aagaggtcgc aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt
     2341 cctgttcttt cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt
     2401 ttttatcaac taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa
     2461 gcccctgaat ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta
     2521 accagttcca aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat
     2581 ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa
     2641 tcgtttctag cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt
     2701 gctcttccaa ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg
     2761 aaacatacgt accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca
     2821 atgcttcgcg aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata
     2881 ttttccttcc aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt
     2941 gtgattgcaa gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata
     3001 acagaagagg tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata
     3061 gtaaaatttt ggttgacata gcgattttta ttgcataaaa ttcacttgaa tacaaaaact
     3121 aatatgttag tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc
     3181 tgaattatct caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa
     3241 tggacaaaaa aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca
     3301 tgcagaaatc gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg
     3361 tgaaacattc gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct
     3421 tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga
     3481 cagtctgtat tgtcatccag attgttctga acactttgat aagatgattc gacactttga
     3541 aaatcctagt ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga
     3601 tacaaataat aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc
     3661 atcacataca atgagtattc ttgctgtgct actttataaa cgttttttat ctggagcttg
     3721 gccgattgca cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc
     3781 tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca
     3841 atacttatcg aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc
     3901 gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat
     3961 tttccaaacc gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta
     4021 tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt
     4081 cttgactgat cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa
     4141 tcctttgcat acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga
     4201 ggagatgaaa gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct
     4261 cccagttgtg gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac
     4321 tgtgcgcttt tctaatccaa atattcctaa tatgcctcag cgaatcgcca cggatacctc
     4381 tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa
     4441 agacccgcag acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat
     4501 tggcattgat gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagacct
     4561 tcagcataag ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa
     4621 aatggctcct attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct
     4681 tggcagaaaa atagaagaaa tgttcatcaa gatggtagca ggaccaaatg ccgtacgaaa
     4741 aacattacag gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga
     4801 tggtacggat cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc
     4861 aaagggattg taggcacatt gatggatata ccagtaggag aggtatggcc tagggagagg
     4921 atcaaagaac taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc
     4981 gtcaatgtgc atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac
     5041 tataagcaga caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt
     5101 atgcctgtat ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc
     5161 acgttagctt ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc
     5221 gaacattcca gcaatgaatt tggattgccg ggatgggaac ctgagcgctt agctcctatc
     5281 aaatccttgc ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc
     5341 tattttctcc aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat
     5401 ccagatgatc cgccatactc gatttttgga ttagctcgaa tcgtaaagaa tcgagaagat
     5461 ttagattggt tgtgtcgcat agtagacagc ccttataacg gaatcactct ttgtacaggg
     5521 agtattgcag aagatccaga aaatgatgta tatgggattt tagcagaatt ttgccaaaga
     5581 gatcgaatcc catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat
     5641 gaggcaccac ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg
     5701 tatgacaacg gatttaacgg atacatccgt cctgatcatg gaagaatgat ctggaatgag
     5761 acaggaagac caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga
     5821 ttatgggaag caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa
     5881 ttttattatg taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta
     5941 ataagcattc ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc
     6001 gattgatcca tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc
     6061 acttttttcc agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga
     6121 gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca
     6181 ttttgtattt tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta
     6241 ctgatataaa aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag
     6301 cgatcataca aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata
     6361 actggtaaca agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt
     6421 tggacttctt tcaatacgct atgttcttga taaagcatga aagtaggaag gggcgtattt
     6481 ggttgattga agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa
     6541 agaatctttt ctttttcagg tataaacaaa tcaaatacag tacaaaggga ctccaccatt
     6601 tctcttgaat aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag
     6661 cttgtttctt ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg
     6721 cgttgttcgc gacaaattgg cagattaggt cgcaaaatat ctacagagag atcaataaaa
     6781 aagtcaatat attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga
     6841 aaattctctt tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt
     6901 cggtcaagta cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct
     6961 tcatcataaa attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca
     7021 tattgaattt gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata
     7081 gacaaaccgt actatgctaa aaatgcatta atctttttca ttttcgtata caaataagag
     7141 gcactatagt ttgtttgctg actgaaactg ataatcgaat ttgtctgaca gctgaacaaa
     7201 gaagtcaaga tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag
     7261 cagtaagccg gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata
     7321 tgcagttctg ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa
     7381 aaatttgatt cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga
     7441 tgttcagata ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagataaac
     7501 gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac
     7561 ttgaaaatat tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag
     7621 aatctctact atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat
     7681 attcaaccat cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga
     7741 cagtccacac agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca
     7801 atcgaattac ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa
     7861 aaggacaaac agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac
     7921 tacaatcagc tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga
     7981 acactttgac tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg
     8041 acttgttaaa accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc
     8101 atttaacgcc gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag
     8161 gagatcttgt tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc
     8221 ctacagaaaa tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata
     8281 cagaaactca accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac
     8341 cagatgtaac tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc
     8401 cagacacaga taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac
     8461 cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa
     8521 acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc
     8581 taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag
     8641 ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca
     8701 gctaccaaaa tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt
     8761 aagtatagca acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt
     8821 gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa
     8881 ataccagatt gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt
     8941 ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc
     9001 taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc
     9061 gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa
     9121 cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata
     9181 aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat
     9241 gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag
     9301 ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac
     9361 aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta
     9421 aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt
     9481 ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc
     9541 catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc
     9601 aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt
     9661 tggctgatgc tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag
     9721 catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat
     9781 gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat
     9841 catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa
     9901 aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa
     9961 acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat
    10021 tatatgatgg aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc
    10081 ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc
    10141 ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg
    10201 ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc
    10261 agcaccttga gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg
    10321 attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag
    10381 acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat
    10441 cacaagatac tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat
    10501 gatttcttta gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc
    10561 catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc
    10621 tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga
    10681 cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata
    10741 gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact
    10801 tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca
    10861 ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg
    10921 acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct
    10981 ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca
    11041 cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa
    11101 tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc
    11161 aaacatatac aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt
    11221 tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc
    11281 ataaataacc aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg
    11341 tcatttcttg aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat
    11401 cagagacgat gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac
    11461 caagagacgt gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt
    11521 cattattgcc tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt
    11581 gatacagttc tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat
    11641 gcataaagat ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc
    11701 taaagaaaac gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat
    11761 aagtaaatcc gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat
    11821 cgatttcttc tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata
    11881 aaacctcttt tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc
    11941 caatctcagc acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa
    12001 cagatttctt tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa
    12061 tagataaaat agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa
    12121 cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa
    12181 tgaggggagg gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc
    12241 ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg
    12301 tttctggaac tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa
    12361 agggtttttt acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa
    12421 taactatagc tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac
    12481 ctccagcagc aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga
    12541 gggcaaaaat aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac
    12601 ttccttaatt cattaataat actcttatct tatt
//
LOCUS       DACCIQ010000069        12556 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCIQ010000069 DACCIQ010000000
VERSION     DACCIQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.071"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            92..940
                     /locus_tag="IVM62_002029"
     CDS             92..940
                     /locus_tag="IVM62_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6845987.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            1137..1910
                     /gene="sufC"
                     /locus_tag="IVM62_002030"
     CDS             1137..1910
                     /gene="sufC"
                     /locus_tag="IVM62_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP6845988.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            1923..3209
                     /gene="sufD"
                     /locus_tag="IVM62_002031"
     CDS             1923..3209
                     /gene="sufD"
                     /locus_tag="IVM62_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP6845989.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            3206..4441
                     /locus_tag="IVM62_002032"
     CDS             3206..4441
                     /locus_tag="IVM62_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6845990.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            4428..4898
                     /locus_tag="IVM62_002033"
     CDS             4428..4898
                     /locus_tag="IVM62_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP6845991.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            4903..6294
                     /gene="sufB"
                     /locus_tag="IVM62_002034"
     CDS             4903..6294
                     /gene="sufB"
                     /locus_tag="IVM62_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP6845992.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(6389..7531)
                     /locus_tag="IVM62_002035"
     CDS             complement(6389..7531)
                     /locus_tag="IVM62_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6845993.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7570..8304)
                     /locus_tag="IVM62_002036"
     CDS             complement(7570..8304)
                     /locus_tag="IVM62_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845994.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8536..8811
                     /locus_tag="IVM62_002037"
     CDS             8536..8811
                     /locus_tag="IVM62_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6845995.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8909..10906
                     /locus_tag="IVM62_002038"
     CDS             8909..10906
                     /locus_tag="IVM62_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6845996.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10982..11317)
                     /locus_tag="IVM62_002039"
     CDS             complement(10982..11317)
                     /locus_tag="IVM62_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845997.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            11444..11659
                     /locus_tag="IVM62_002040"
     CDS             11444..11659
                     /locus_tag="IVM62_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6845998.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            11696..12058
                     /locus_tag="IVM62_002041"
     CDS             11696..12058
                     /locus_tag="IVM62_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP6845999.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            12104..12430
                     /locus_tag="IVM62_002042"
     CDS             12104..12430
                     /locus_tag="IVM62_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846000.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         4196 a         2141 c         2550 g         3669 t
ORIGIN      
        1 ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag aaaacagaca
       61 aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg gatttgcagc
      121 agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca gcaaagattc
      181 agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac cgcacgcaga
      241 gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg aaatcgtcaa
      301 attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg atgccaatta
      361 cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg actttgtcaa
      421 tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca aagatgtttc
      481 cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact ggggacgcat
      541 catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag atctggaaac
      601 agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc atacgatcga
      661 cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg caatcaacgc
      721 aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc ttgaaaagga
      781 caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca gtgacaaaat
      841 caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca cagacaaatg
      901 gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac aaaaaagatg
      961 agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta atttagaaat
     1021 caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt cattgcgctt
     1081 cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta taatgcatgt
     1141 ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc cttaaaggcg
     1201 taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac ggcacaggaa
     1261 aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca gaaggcgaaa
     1321 ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg cgacttggct
     1381 tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg gaattcatgc
     1441 gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg gcatttttga
     1501 aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct gaacgttatc
     1561 tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa ttgttgatgt
     1621 tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc gatgctttga
     1681 aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca ttgatcatca
     1741 cccactatca acgattattg aactatatca cacctgatgt cgttcacatc atgatggaag
     1801 gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca gaaggctacg
     1861 ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa gggggagcaa
     1921 gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt tctttaagta
     1981 aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa gcagatgaac
     2041 tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt gatgtgaaag
     2101 aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg aaagacaatc
     2161 ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg gaattagcag
     2221 aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca gaactagtcc
     2281 aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact gcactccatg
     2341 tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg atcgatgaac
     2401 cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc aagcatgtgt
     2461 tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag acaaccaaag
     2521 aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa gctggttcaa
     2581 aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat atgaatcgcc
     2641 gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg aatgacggtc
     2701 atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca gaagtcaaaa
     2761 tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta accaataaag
     2821 cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa ggaacattga
     2881 cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc caacaagaaa
     2941 gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt cttttgatcg
     3001 atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat ccagaagaaa
     3061 tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta gtgattcgag
     3121 gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa gaattagtcg
     3181 aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca atgattttcc
     3241 tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg cagcgacgac
     3301 ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg ataacgccaa
     3361 tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg aacaagcaag
     3421 agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt ttactcgtgg
     3481 tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata tcaaagcagg
     3541 agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt ggcagcagtt
     3601 ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag gcttcctgga
     3661 tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca ttgctcatgt
     3721 atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg tccatgaaaa
     3781 cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca ttgatgtcca
     3841 agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac caacaggtat
     3901 tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg aatttggtgg
     3961 cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac cttggaaatt
     4021 cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa tcgattattt
     4081 gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg cttatgtgtt
     4141 gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc cgaattttcg
     4201 gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg cgacagcttt
     4261 agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc tattgaagta
     4321 ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa cgcaagacgc
     4381 cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg ggactatcta
     4441 aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg catcatcgtg
     4501 gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt ggagatgtga
     4561 tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt gacggcagtg
     4621 gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc ggaaaatcaa
     4681 tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga aaagaagtca
     4741 aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa tttcccgctc
     4801 ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa aacgatggta
     4861 ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt accagaatta
     4921 gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac aggagaaggc
     4981 ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga atggatgctt
     5041 gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac atggggcccc
     5101 gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag tgacaaaccc
     5161 gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa gattggtata
     5221 ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc agaagtcgtg
     5281 taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga cactgactct
     5341 gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt accgccaacc
     5401 gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt tatttacgtg
     5461 ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa tgcggaaaac
     5521 acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat ccactatgtc
     5581 gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat cgtagaaatc
     5641 tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc agacaatgtg
     5701 tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga atggatcgac
     5761 ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga tggaaaaggt
     5821 gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca agatacgggt
     5881 gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa atcaatttca
     5941 aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa cagtgcgggt
     6001 tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa atccgatacg
     6061 atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc caaagtctca
     6121 aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga atctgaagca
     6181 actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc aatggaatat
     6241 gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg gtaaacctgt
     6301 tataggagct agaacccttg atatataagg gttctagctt ttttgttttg cctgtatact
     6361 agaaatgacc agattttgac cagctctttt actgatgagc catcatatca gcaaagtctt
     6421 gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat gtcactttac
     6481 tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct tctaataata
     6541 aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta tattttttca
     6601 aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt ttaactgttg
     6661 taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac tcttcttttt
     6721 gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag ataactcgtc
     6781 tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct acagcaagag
     6841 ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt aaagcgagta
     6901 cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat tgtttcatgt
     6961 taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag taaaatttac
     7021 gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga ttattgtcaa
     7081 tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc atataatcgt
     7141 aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg acagagatat
     7201 tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat cttactgaga
     7261 ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca ttatacaggt
     7321 cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga tgtctgtact
     7381 cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta gtatttattt
     7441 tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc acttgaccgt
     7501 ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt ttacaaggag
     7561 tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt ttttcccaat
     7621 tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca cagcttcttc
     7681 gggataagac atgtctttca attgaatagg tagccattca tctaccattc tttctagtag
     7741 ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta caatagcatt
     7801 gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat ttttaacttt
     7861 attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg aatatctatc
     7921 cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat gctggaacag
     7981 gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa gaataattcc
     8041 caaaatatat tcttctacat tgccacaaac taactcctct atactcatat ttcccaaatc
     8101 agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc attcgtagat
     8161 agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg gttgtgttaa
     8221 ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc gttctaaata
     8281 atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta tacctcaaaa
     8341 aaggggtgtt gtaaataaat tttacaaagc tgttgacacc ccttttttat cgaggtatta
     8401 tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa taacaaaata
     8461 cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca agcaatcacg
     8521 aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat acaagttgaa
     8581 caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat tatttctgga
     8641 gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt atcagtagga
     8701 acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga tacggtcatt
     8761 agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg agtaaacaaa
     8821 taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt tttagtcttt
     8881 aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata ttccaattta
     8941 tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa ctgatgtaag
     9001 agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag agatactaaa
     9061 ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca aggagagttt
     9121 tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg agaaggggaa
     9181 ggtagtagat ttgagtaaag atgatagtag atacttaagc atagaaaagg ttttatctat
     9241 caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact ctgaaaatct
     9301 agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg gtaaaatggt
     9361 tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg taaatgcttc
     9421 gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta atgaagacaa
     9481 catgttagat attactgagc ttccgattga ttttaccatg acatgttcta aaaattgtca
     9541 aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta agttgataaa
     9601 aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg attcctccga
     9661 catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata aattacttaa
     9721 gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg ctagtatttt
     9781 taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg atatcagtga
     9841 aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag aaacatttaa
     9901 ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga aagaggaatc
     9961 aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga caataaggaa
    10021 ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta atttttttga
    10081 tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta aaaaaatgag
    10141 ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat cacttatgac
    10201 ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga tgctcgaata
    10261 cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga gaagcaatgt
    10321 ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg aagatatcct
    10381 tccacttctg gaaaaaattc attttacata tcaatatttt tcttatgaat gggttaagat
    10441 atgttttaac gaaaaagagg ctaaaagagt atttcctcaa aattcccgat cagaactcag
    10501 caaggaaaaa caattgattt tagatgaagc acatgaagtt ttacagggct tatcgtggca
    10561 agggacatat ctaatgacag agaaagaact tattaacact gtttgtgaac gcctacgtaa
    10621 tgttggggat aacagcgtga aaaatgcttt aataaaaaac agaggctact ttgttaaaca
    10681 aggattcact cttgaacgag cttctaagga tattaagaaa ctatacaatg tgaaagcaag
    10741 tagtagtttt ttaatatatt tagcgaaagg ggcagggata gatgaaacaa taaaagaatt
    10801 gatattatca ggagctggga tagttcctct gtatcaattt acaagtgtaa caagagatga
    10861 tggacggaga gtaaggaaga gaaaagaccc aattgtatta aactagacag gttatccata
    10921 gctcttaatc ttatttcatc tcaatacacc caccgttaaa gttcggtggg ttatttattt
    10981 ttcaatcttt caatagctga ttgcgttcat tttcttgcct ttttaatagc ttcatatagt
    11041 caattgttcg aactgcatct tcagtgctaa gagaacgata gagagccaaa aactcttcct
    11101 cataatctac gacaacattg tctagaaaat ggcttacagg ggttcctagg gctctagaaa
    11161 gagctgttaa gctatcgagg ttaggctctg ttacagaatt ttcccatctt gctactgcct
    11221 gttgtgtaga gaagattttt tcagctaatt ctttttgtgt ccacccgaga gcttttcgct
    11281 tttctctaat gacctttgca tattgtttct gtttcattaa tacaccccca tctcagtata
    11341 gtacaacaag ttgttgtatt tgtaatggta tttgaagttt tttatatttt atgagttgaa
    11401 atacatcata ttgttgtata taataaaaca ggaggggaga atcatgaaaa aagtaagtaa
    11461 tctcagccga tacagaaaag aaaaaggact ttctcaaacg gagttagcaa aaaaaatgaa
    11521 tgttacacag caatgtgtaa gttcgtggca aacagggaga acgattccta aaccctatca
    11581 aatgaagatg ctttctgaga tattaagtgt accaatgaat gagctgtttt ctgatgtatt
    11641 taataaagta aacagttaac taataagcaa ttgaatgatt ttaggaggag aagacatgta
    11701 ttgtagaaaa tgtggcaaag aaataacaga tgaaagtaag tattgccagt tttgtggagc
    11761 actacaagaa acagatgaga caaccggaac agacacagta aagaaaagta ctgagcagac
    11821 aattaatgaa atgaaggaac aaatcgtaca tggacaagag acagggaagc tgtatttgat
    11881 tattggttgg atttctatgg tagtatcgtt agcatttatt cctgttgtat ttggtgctgt
    11941 tgctgttatt atgggctact tatatcgaga aaaagatgag aaaatgggta cgattttgat
    12001 gatagcaggg gtagctggag caatctttgg tgtactttta gggatgtcaa tgtactgata
    12061 agagggataa gaacaaatta ttttggagat aggagataaa aagttgaata aagtaaatat
    12121 gaacaagaag tttgtcatta taggtattgt tgtcttggct attataggta ttattttttc
    12181 aaaaatgaca ggtacatctg gattagagaa gtcatcaaaa ccattggtaa cacagatttt
    12241 acaagaacag tacaacttaa atcgatcatg tgatgatgtg acgattactc atgaaaatgg
    12301 agataacaca taccgggcta aagctatatt agacaatgga agtgctatca acataaatat
    12361 tgagtactat ccaaagaaag accgtatata tgtggagata ccatatgctg aggtgttaat
    12421 gttaaattga tttaaatgta ttatgcatac cgtccaaaca ggaactccca aaggtgtaaa
    12481 ttcactaggg agtttttata gtttcctata aattaaatac taatacaggt gacaaaaaaa
    12541 tattacaatg attgtc
//
LOCUS       DACCIQ010000070        12281 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCIQ010000070 DACCIQ010000000
VERSION     DACCIQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.072"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(59..1042)
                     /locus_tag="IVM62_002043"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IVM62_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IVM62_002044"
     CDS             complement(1437..2279)
                     /locus_tag="IVM62_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6846001.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IVM62_002045"
     CDS             2604..3020
                     /locus_tag="IVM62_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6846002.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IVM62_002046"
     CDS             3017..3487
                     /locus_tag="IVM62_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6846003.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IVM62_002047"
     CDS             3501..4274
                     /locus_tag="IVM62_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6846004.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IVM62_002048"
     CDS             4288..5100
                     /locus_tag="IVM62_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6846005.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IVM62_002049"
     CDS             5154..6119
                     /locus_tag="IVM62_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846006.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IVM62_002050"
     CDS             6256..8979
                     /locus_tag="IVM62_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846007.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IVM62_002051"
     CDS             8992..9624
                     /locus_tag="IVM62_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6846008.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IVM62_002052"
     CDS             9829..10056
                     /locus_tag="IVM62_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP6846009.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IVM62_002053"
     CDS             10125..10391
                     /locus_tag="IVM62_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6846010.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IVM62_002054"
     CDS             10381..10650
                     /locus_tag="IVM62_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6846011.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="IVM62_002055"
     CDS             complement(10829..12130)
                     /locus_tag="IVM62_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6846012.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4007 a         1989 c         2407 g         3878 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaaa
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
    12181 ctgtatgttt tgttttttca ttctcaaaag ggaccgatag aatgattttc tatcagtcct
    12241 agaaattata gagccttttt tatttggtct tgaagaagaa a
//
LOCUS       DACCIQ010000071        12148 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCIQ010000071 DACCIQ010000000
VERSION     DACCIQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.073"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..1200)
                     /locus_tag="IVM62_002056"
     CDS             complement(<1..1200)
                     /locus_tag="IVM62_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6846013.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGY"
     gene            complement(1203..4592)
                     /locus_tag="IVM62_002057"
     CDS             complement(1203..4592)
                     /locus_tag="IVM62_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6846014.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4849..6240
                     /locus_tag="IVM62_002058"
     CDS             4849..6240
                     /locus_tag="IVM62_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6846015.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(6313..6960)
                     /locus_tag="IVM62_002059"
     CDS             complement(6313..6960)
                     /locus_tag="IVM62_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6846016.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(6968..7654)
                     /locus_tag="IVM62_002060"
     CDS             complement(6968..7654)
                     /locus_tag="IVM62_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846017.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(7660..8733)
                     /locus_tag="IVM62_002061"
     CDS             complement(7660..8733)
                     /locus_tag="IVM62_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6846018.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            8955..9629
                     /locus_tag="IVM62_002062"
     CDS             8955..9629
                     /locus_tag="IVM62_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6846019.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            9634..10974
                     /locus_tag="IVM62_002063"
     CDS             9634..10974
                     /locus_tag="IVM62_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6846020.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(11123..12109)
                     /locus_tag="IVM62_002064"
     CDS             complement(11123..12109)
                     /locus_tag="IVM62_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP6846021.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         3470 a         2297 c         2198 g         4183 t
ORIGIN      
        1 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
       61 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
      121 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
      181 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
      241 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
      301 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
      361 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
      421 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
      481 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
      541 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
      601 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
      661 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
      721 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
      781 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
      841 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
      901 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
      961 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
     1021 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
     1081 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
     1141 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
     1201 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
     1261 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
     1321 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
     1381 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
     1441 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
     1501 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
     1561 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
     1621 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
     1681 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
     1741 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
     1801 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
     1861 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
     1921 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
     1981 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
     2041 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
     2101 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
     2161 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
     2221 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
     2281 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
     2341 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
     2401 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
     2461 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
     2521 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
     2581 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
     2641 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
     2701 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
     2761 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
     2821 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
     2881 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
     2941 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
     3001 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
     3061 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
     3121 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
     3181 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
     3241 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
     3301 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
     3361 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
     3421 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
     3481 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
     3541 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
     3601 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
     3661 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
     3721 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
     3781 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
     3841 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
     3901 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
     3961 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
     4021 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
     4081 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
     4141 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
     4201 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
     4261 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
     4321 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
     4381 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
     4441 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
     4501 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
     4561 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
     4621 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
     4681 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
     4741 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
     4801 gagggagaaa aacatatgat caggttgttg gaatgaaact atagaaagat ggaaaaaatc
     4861 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
     4921 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
     4981 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
     5041 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
     5101 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
     5161 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
     5221 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
     5281 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
     5341 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
     5401 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
     5461 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
     5521 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
     5581 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
     5641 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
     5701 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
     5761 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
     5821 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
     5881 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
     5941 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
     6001 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
     6061 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
     6121 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
     6181 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
     6241 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
     6301 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
     6361 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
     6421 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
     6481 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
     6541 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
     6601 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
     6661 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
     6721 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
     6781 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
     6841 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
     6901 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
     6961 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
     7021 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
     7081 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
     7141 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
     7201 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
     7261 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
     7321 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
     7381 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
     7441 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
     7501 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
     7561 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
     7621 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
     7681 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
     7741 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
     7801 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
     7861 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
     7921 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
     7981 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
     8041 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
     8101 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
     8161 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
     8221 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
     8281 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
     8341 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
     8401 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
     8461 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
     8521 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
     8581 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
     8641 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
     8701 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
     8761 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
     8821 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
     8881 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
     8941 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
     9001 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
     9061 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
     9121 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
     9181 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
     9241 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
     9301 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
     9361 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
     9421 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
     9481 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
     9541 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
     9601 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
     9661 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
     9721 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
     9781 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
     9841 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
     9901 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
     9961 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    10021 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    10081 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    10141 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    10201 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    10261 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    10321 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    10381 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    10441 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    10501 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    10561 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    10621 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    10681 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    10741 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    10801 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    10861 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    10921 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    10981 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    11041 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    11101 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    11161 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    11221 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    11281 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    11341 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    11401 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    11461 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    11521 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    11581 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    11641 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    11701 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    11761 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    11821 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    11881 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    11941 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    12001 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    12061 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    12121 ctagcaaata gatttatgat tggtatat
//
LOCUS       DACCIQ010000072        11595 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCIQ010000072 DACCIQ010000000
VERSION     DACCIQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.074"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(146..2221)
                     /locus_tag="IVM62_002065"
     CDS             complement(146..2221)
                     /locus_tag="IVM62_002065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP6846022.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2223..3140)
                     /gene="glyQ"
                     /locus_tag="IVM62_002066"
     CDS             complement(2223..3140)
                     /gene="glyQ"
                     /locus_tag="IVM62_002066"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP6846023.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3526..4344)
                     /gene="recO"
                     /locus_tag="IVM62_002067"
     CDS             complement(3526..4344)
                     /gene="recO"
                     /locus_tag="IVM62_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP6846024.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4487..5386)
                     /locus_tag="IVM62_002068"
     CDS             complement(4487..5386)
                     /locus_tag="IVM62_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP6846025.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5401..5802)
                     /locus_tag="IVM62_002069"
     CDS             complement(5401..5802)
                     /locus_tag="IVM62_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP6846026.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5780..6256)
                     /gene="ybeY"
                     /locus_tag="IVM62_002070"
     CDS             complement(5780..6256)
                     /gene="ybeY"
                     /locus_tag="IVM62_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP6846027.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6285..8477)
                     /locus_tag="IVM62_002071"
     CDS             complement(6285..8477)
                     /locus_tag="IVM62_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP6846028.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8501..9472)
                     /locus_tag="IVM62_002072"
     CDS             complement(8501..9472)
                     /locus_tag="IVM62_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP6846029.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10177..11556
                     /gene="brnQ"
                     /locus_tag="IVM62_002073"
     CDS             10177..11556
                     /gene="brnQ"
                     /locus_tag="IVM62_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6846030.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3139 a         2450 c         2133 g         3873 t
ORIGIN      
        1 ctcacttttc atgagcgttt tgaaaattcg aacaaagaat aaaataacaa aaattattaa
       61 aatagaatta ataacagcta tttataacaa aaaaaacaga gacacagcac gtttcgtgca
      121 tgctctctgt ttttcaaaca aaagactatt ttgtgatgat ttgatccaag cttgctaatg
      181 acaaagccat ggcagctatt tgacttagtt gtgttagtcg gttcttacgt actgctggtt
      241 catcagccat gaccatcgtt tgatcaaaat aattttcgat cagtggacga agagcgacaa
      301 gctggtcgta gttttcgtgg atcgtttggt cagcaaaact tgctttcatt gcttctactg
      361 ctttgtgtaa ttctttctct gcgtcgtttt cgaacagctc agggtctact tctgattgtt
      421 tttcaagctg cgcttttttc gctaagttca cgacacgtgt caaagcttcc attgaaggtt
      481 tgaagtcttc atctgcaaga tgttgtttga acaattgtgc tgctgcaaat acttttgtca
      541 gatctttttg ttccgcattc aaaacagcat cgatgacatc atgacgaata ttcttcgttg
      601 tcaataattg gcgcagacgg cctttgataa agtcgatcac ttgttgttgc ccttctgata
      661 aaccaatgcc gaaacgatcc gtatctttac tgataacatc gctaatcgtt tcttgcagaa
      721 cagacaaagg aaaggcccat tttttcgatt cgatgatccg aacgacaccg tatgcttggc
      781 gacgtaaggc ataaggatcg tttgacccgc ttggaatcat cccaactgca aagaaagaaa
      841 agatgctatc taatttatct gcaatcgcta aaacagaacc tactgtagac tgaggtagct
      901 ctccttcact ggatataggc atgtaatgct cacgaatcgc tgtcgctaca gcaggtttct
      961 cgcctttcag aagagcatat ttttcgccca tgatcccttg tagttcagga aattccccta
     1021 ccatgttggt tactagatca aatttataga tttcagcagc tcgttgcaaa tccatcaatt
     1081 cttcctcaga tagtcctact tgttcgccaa tgatacgggc aatgacttgt acccgttgca
     1141 ttttctcata gatactaccg attttttcat ggaaagtaac agtttttagt ttttcaacgt
     1201 attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct tctaaacgag
     1261 ccgtcaatac tttttgattg cctcgaacca catcttccaa ttttacccga tcaccattac
     1321 gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg aaataacgtt
     1381 gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat ttttcttcga
     1441 agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct agtaaatctt
     1501 cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt gtaacgatca
     1561 tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt tcttcatatt
     1621 cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga gaggctctgc
     1681 ctgttttgac atccaagatt tcgaatggga ttacttcgtc atctaacaat gccacgatcc
     1741 agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt acagggaaag
     1801 tcaaactttt gatcacccct gctaagcctg tcaatacttc ttttgcttcc ttaccatgag
     1861 tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg atagcgtctg
     1921 tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt ccatctgcgt
     1981 ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct tgtcgttctg
     2041 gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg atcgtatcat
     2101 aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt ttcatgcttg
     2161 gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag tcttttgcca
     2221 tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa caaagggaat
     2281 cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat gttgcggatg
     2341 cgagaaagat aaccggcacg ctctgttacg gatactgcac cgcgggcgtc taataagttg
     2401 aatgtatggc tacatttcaa gatataatca tacgccggat gaacaagatt ttcatcgata
     2461 cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc ttggtcgctt
     2521 acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc gccatatttc
     2581 acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata agaagcaaga
     2641 cgttctaatc cataagtgat ctcagaagtt actggtttac aaggcagtcc accaacttgc
     2701 tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca acccacaccg
     2761 gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc gtgttctaat
     2821 ggatcgattc ctaaaaggcg caagctttct aaataaagct cttgaatatt ctcaggtgaa
     2881 ggtttcatca ccacttggaa ttgatgatgc tgatacaaac ggtttgggtt ttcaccataa
     2941 cgtccatcag ctggtcggcg agatggctct acgtaagcag cattccatgg ttctggtccg
     3001 attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt gtcataagct
     3061 tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat catatcttgg
     3121 acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt gctgtcaaat
     3181 aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac atagggacga
     3241 ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt tatcgtactc
     3301 gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac tcgctaaacc
     3361 acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt cttgtttcta
     3421 taatagatcc tatcactgaa cgatctattt aatctatggt taataataga cggaactccc
     3481 tgtgtttgtc aaatggtttt gattttttgt tgtatgtttt atttactatt cttctttttc
     3541 aggtgtctct ttttcttttt cagacatgtc tttctctgac gtctttaata cgtgtgccca
     3601 agacttcatt tgattgatga actttttgct tttcaaatgg ataccgacat attcatcata
     3661 cagctggtcg atgagttcac ggattgcttg tttggtttct tctttcagat ggattccact
     3721 aattttatca taagaaatag ctgaaaacat ccgaacgaaa taaacagctc gaggatctgc
     3781 atgataacgg tgatcgtcca tcgaccaatg tctttcgcat atcacaccac catatttgga
     3841 agaatagtca aattttcctt ttgtttcccc gcatacgacg cagtgtgtcc agattggtgc
     3901 aattccgaag cgttgcagta tttggacttc aaaaatattc gtgatgattt ctgcatcgtc
     3961 tccttggtcc atccgttgca gtgcttgttg cgtaaactgg aacaaatgcg ggtcgtactg
     4021 atgatcttcg attgccgcat ctactagatt caataaatat gtgccatagc cgctcaaaaa
     4081 gatgtcttct tggatgtgtc gaaacgcatg gacatctttg acactgctta aaaatgacaa
     4141 accttcactt cgaaaattcc cgatataaac tgcttcagta aaagggagca aagccggagc
     4201 taagggctga ttttgccgat ggatctcttt gatataaaac atcattttgc cagaagattc
     4261 tgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata aaatcaagcc
     4321 ttttgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg gaaaagcaga
     4381 ttctgccgat ccagatcatt tatgttgcga tcttccgcag cttttctcaa cacgcggatt
     4441 tttattcagt ttatttctat acctaattta caagaagctc agaatatcag tattcgtctt
     4501 ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt tgaactttga
     4561 cccaaagttc tagaaagact ttgtctccta aaagattttc gatatctttt cttgctttcg
     4621 ttcctacttg cttcagcatt tttccgcctt tgccgataat gatccctttt tggctatctc
     4681 tttcgacaat aatcgtagcc tggatatgga ttttatcatt ctcgtttcgc ttcatcgaat
     4741 caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag actttctcac
     4801 ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca tctggaaaat
     4861 attgtgggcc ctctggcatt tcactgacta atacatccat caaacgttcg acgttgtttc
     4921 cttctgtagc agagattgga atcacttgtg caaaaggcat ctgcgttgaa taatcttcga
     4981 tgattcccaa caactcgtct ggatggattt tgtcgatttt attgataatc agataaacag
     5041 gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccgaccg cgtttttgat
     5101 cagcactaat cataaataag accgcgtcca cttctttcaa cgcactgtaa gctgcttcta
     5161 ccataaaatc tcctaaacga tgctttggtt tatggattcc tggtgtatca ataaaaatca
     5221 actgagcttc tggtgtagtg tatactcctt ggattttatt tctagttgtc tgtgccttgt
     5281 cactcatgat ggcaattttt tgacccacga ttcgattcaa taatgtggat ttccctacat
     5341 ttgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatat gttctccttt
     5401 ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa cgatgacggt
     5461 aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt tcccaatcgg
     5521 atgaaaatga aaatccgtaa acatgtccac gacattttca aaaacagtat tgatgatctc
     5581 tactatccat acgagaaaaa cagctaaaag cagccataac cattctatcc gatcaagttg
     5641 gaaaatcacg cccataaaca aagcgaggat tccgaacgca acatgtttcc tcatgttgcg
     5701 ttcttcctca aaaacagttt tgactccttg gacggcaaat tccacggaca tcaggaaatg
     5761 tttgtttttc tctacgtttt tatcttttaa gtccataggc atctaagatc tctttctgta
     5821 aaccaaacat ttctttctca tcctctggtg tcatatggtc atacccgttg atatgcaaga
     5881 atccatgaac cgctaaaaag ccaagttccc gatcatacga atggccgtaa gcttctgctt
     5941 gctctgcagc acgttcagta gagatcatga tgtctcccag tacacgaggc aatggattct
     6001 ctccatcttc aaaaatcact gggatttcgt cttcgccctc ttcttccaag gcaaaactga
     6061 tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg attgctgcat
     6121 tgtccatgaa agtaacagac atttctgttt cttctggcaa ctctaaatat gatgctgcaa
     6181 attgcagcaa gtcgtcgatt tctttcattt tctcttctga gacttggttt gtttcatcca
     6241 tgaaggtaat atccattata catcgcttct ttctattctt cttgctattc ttcttgttct
     6301 tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga atggaaggta
     6361 ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa cgtcaatcca
     6421 ctgtcgtcca attgtccatc ggaaatacgc tcttcaataa gattatggac aaaactttcg
     6481 attttttcgc tcgttggatg gtccattgcc cgtactgccg cttcacaact gtcagctata
     6541 ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa ttgctcttcc
     6601 gtaacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag cgtggttcca
     6661 tgatgctgtc tacaaatatc gatcaccatt tgcggcatat tataatcttc caatattttg
     6721 gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa taagaaattg
     6781 tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg tttgatcttg
     6841 ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc gatttctgct
     6901 acagcattag cacttagatt ggctaccatc atgctatggt gatacgtacc tggtgcttct
     6961 tccagcaact gtttcaataa aggatgattg ggattgctga gttcatttaa tacgatcaca
     7021 ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag caagaatgaa
     7081 aacagtgcac ctgtgaatcc gcagactagc gttaaccaag tcgttgcatc accaaagctc
     7141 attccttgat agacgatcaa aatgacatcc atcatcacag gaaaaataat gacccacatg
     7201 gcagcagata atccttgttc tgagatccgt ttacgtctga caatcgttgc catgagccca
     7261 gagaaaacat aagacatcaa gataatcgtt agaaagttcg tgccaatcga atcataaaaa
     7321 atgaaaagtg cggatactgc ttggaaaata ccagccatga tccctgctct tcgattcaag
     7381 aaaaagctta aaacaagtgg tgcaaaagca gcgggataaa acagcgggat ataagtagct
     7441 tgttccgttt ggaataattg gaaaaatttc atcagcacta tgctgactga catagctgtc
     7501 acatagaaaa gaacgtattt tgttctttct cctgtatttt ggaattgaag tgaattataa
     7561 atcagtacgc cgatttgaag caaaacagct aaaaccatgg caattagcgg aaaaatcgaa
     7621 gttgtttgat tagtcagtcc tagtaattcc aattttttca tagctgaagc atcaatttga
     7681 ttaccttcac gaacaatgat ttctccttgg aagatcatca ctggttgtac agcttctcgt
     7741 gcggattgtt tcaattcttc tgtctttttc tcattcaata cgtcattcac tacgatacct
     7801 tgatccacta agtagcgaat catttgttgt tgtgctggtg tgacattcaa atattggatt
     7861 tgctcttctg cttcttgttt aaaagcttcc aattcacttt cacgaacttg tttgctcatc
     7921 tgttcgtcaa tgagcttcag actctcatcc ctcacagtat gaatctcagt ttcagtcatt
     7981 tcaaatatcg tcgaataaaa attgttcggt agtttttgat aaaacacaac attttctgca
     8041 ttcacatttt caaaattttt cttcagcgcg gcgatccgct cttctgtagt aggctttggg
     8101 atattttcat cttcctttgc cttatcaatc ttttcgtcat aagatttgtt gatggcatca
     8161 ttcgtcttat cgatcaattc aaatagttgc ttgatccgat tgttctggtc atctgcaaga
     8221 tctttttggt aagtgtattc tggagtgact gcttctgctg ccagttttct tttttgttcg
     8281 gtttcttccg tgttctcgat cgttttgttc gcacggatac tttcttctgc tacttggcct
     8341 tctctgaaat cattggcctt ttgtttgaca ctactaaatg taacgccgca gataatgata
     8401 aaaaacaaga acagcaggaa aggcaatgct gcctttccaa gtttttcttg tagtttctgc
     8461 agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga tggagatctg
     8521 ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg tcacttgctt
     8581 caaaattaac aaaatcgatt ttcttgattt gctttaatgt tcgttctgca tgaacaagac
     8641 cacttgttgt ccctcgaggc agatcaatct gactcgtatc tccattgaca atcatttttg
     8701 attgataacc aagacgtgtc aggaacattt tcatctgagc aactgtcgtg ttctgtgctt
     8761 catccaaaat aacgaatgca tcatccaatg tccgcccccg catgtaagct aaaggagcaa
     8821 tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc taatccaaaa atttggtata
     8881 aagcatcata aacaggacgc agataaggat ctactttttc ttttagatcc ccgggaagaa
     8941 agcctagatt ttcccctgct tccacagcag gccgcgtcaa aatgattttt tgcacttcac
     9001 cttttttcaa ggcagcgatt gctaatacta ctgcaagaaa agtttttcct gtcccggctg
     9061 gtcctacgcc aaatacgata tcatgtttgg ctaccgattc gacatatttc ttttggccgc
     9121 tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc tcatacatct
     9181 caacaaaata atctaatgtg cctttttgcc ccattttcag tgccgtcacg acatctggtg
     9241 tacttacatg gattcctcgt ttgatcaatt cctgcaatgt ccgaatgact gaggcagcca
     9301 aggaaatctg ggattgttcg ccaatgatct gaatgatttc tccacgtgta tggatggttg
     9361 tttctgttgt ttcttcaatc agttttatgt gcttgtcgtg tgcacctaaa agcatgctga
     9421 catcatcagc agccgtcaat ctaatctcta aagaactaga ctcttccgtc aaaaatagac
     9481 atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa actatatagc
     9541 aaataagaaa aggttctaaa agagttgctt aaccataagt accaagaaac gttaaaaaat
     9601 agctgcttcc attctctaaa ggtctcgttt gaataaaaaa agctgatcat gtaagctatg
     9661 cataagatag aggacaagta ttccgtctaa cttagcaagt attctctggg tatttttctt
     9721 gtttcaaaaa aagaaaacaa aatatttgaa atattcttgc atttcaaaaa aaacaggtgt
     9781 attataacgt caacaaataa atatacagca aagcgtcgag gaaaacagta ggctattcag
     9841 tgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat agacgaacat
     9901 catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta tcaggccagc
     9961 ggataacttt atcgaaaagc gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg
    10021 taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt tatattttaa
    10081 ggtggtggtt ttcatggaat ctgatattag tggtatttct agtttctgca acgtttattt
    10141 tgttactcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10201 tggaagcaat tattgatggt cagttcactt atcttcggga tgttcttcgg gtcaggtaat
    10261 ctgatctttc ctgtccactt aggtcaaatg gctggatcaa actggatcag cgctggtgta
    10321 ggatttgcca tctcaggcgc tcttttccca ttattagcta tcttagctgt agtagtgaca
    10381 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10441 ttgatccttg ttcatttaac gatcggacca ttctttggta caccgcggac agcagcaact
    10501 gcttttgaaa tggctgctaa accctttttg cctgaaaaat atgattcatt aggtatgttt
    10561 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtacatcc aacacgtttg
    10621 gtcaaatatg tcggaaagta tttaaactcc ttgtttcttg cattattagc tatcattttt
    10681 gtcgtttctt tattccaacc catggggaat ctaaaccaat ctgctgcaga ttcttatcag
    10741 acgaacgctt tgacaaatgg cgttttagaa ggatacaaca cggttgatgc agtagcttta
    10801 cttgctttaa gtgtcacttt tgtccatgca gttcgtgatc ttggttttaa agataaaaaa
    10861 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgttagaagt catgatttat
    10921 tttggtctgg tccttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    10981 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggtgc tagcttctta
    11041 ggggtaatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11101 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11161 tttgtctctt ttgtcgttgc gaatgccgga ttggataata tcatccaatg gtctttacca
    11221 gtattgatgc ttttgtatcc attgtcactt gcgttaatct tgctttcctt aacagcaaaa
    11281 ttcttccaaa agagtccatt tgtctatcaa gtaacgatgt tcttttctgc cgttccagcc
    11341 gtattggata tgttggcaaa ttcgcctgca ttagtgagtc agcaaactgt cgtagcaagc
    11401 ttactaggat tttatcatca ttatgttcct tttgctgcat taggacttgg ctgggtcgtc
    11461 ccaacgttgc ttggttacgc tggcagcttg ctcttttatt atgtatatcg cgtatcagga
    11521 tataaacaag aagcaagtga gttaccagaa gaataaccaa atcaaacaca atttaacata
    11581 agagatatta aacag
//
LOCUS       DACCIQ010000073        11545 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCIQ010000073 DACCIQ010000000
VERSION     DACCIQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.075"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(105..1568)
                     /locus_tag="IVM62_002074"
     CDS             complement(105..1568)
                     /locus_tag="IVM62_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6846031.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1632..4649)
                     /locus_tag="IVM62_002075"
     CDS             complement(1632..4649)
                     /locus_tag="IVM62_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6846032.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4800..5639)
                     /locus_tag="IVM62_002076"
     CDS             complement(4800..5639)
                     /locus_tag="IVM62_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6846033.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5784..6029
                     /locus_tag="IVM62_002077"
     CDS             5784..6029
                     /locus_tag="IVM62_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846034.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6092..6514
                     /locus_tag="IVM62_002078"
     CDS             6092..6514
                     /locus_tag="IVM62_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846035.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6608..6925)
                     /locus_tag="IVM62_002079"
     CDS             complement(6608..6925)
                     /locus_tag="IVM62_002079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846036.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6952..7158)
                     /locus_tag="IVM62_002080"
     CDS             complement(6952..7158)
                     /locus_tag="IVM62_002080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846037.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7139..7321)
                     /locus_tag="IVM62_002081"
     CDS             complement(7139..7321)
                     /locus_tag="IVM62_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846038.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7335..7685)
                     /locus_tag="IVM62_002082"
     CDS             complement(7335..7685)
                     /locus_tag="IVM62_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846039.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7711..8013)
                     /locus_tag="IVM62_002083"
     CDS             complement(7711..8013)
                     /locus_tag="IVM62_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846040.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8016..8249)
                     /locus_tag="IVM62_002084"
     CDS             complement(8016..8249)
                     /locus_tag="IVM62_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846041.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9235..9557)
                     /locus_tag="IVM62_002085"
                     /pseudo
     CDS             complement(9235..9557)
                     /locus_tag="IVM62_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9680..10795)
                     /locus_tag="IVM62_002086"
     CDS             complement(9680..10795)
                     /locus_tag="IVM62_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846042.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10801..11199)
                     /locus_tag="IVM62_002087"
     CDS             complement(10801..11199)
                     /locus_tag="IVM62_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846043.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3053 a         2370 c         1769 g         4353 t
ORIGIN      
        1 gagatttcaa attaaaaaac gaaaatgaga gatacaaata tatcaaatat ttaaaaagta
       61 accacatttt ctataccact ccttatcaga aacagcctta ttctttagaa taagactgtt
      121 tctgggttga ttaatttgtc ttttgttaaa ctaccatcta aggcaagttc taaatgtaaa
      181 tgtggtcctg tactatttcc tgttgaaccc acataaccaa taatttgtcc ttgttcaact
      241 gtttgtccta cgttcacgca atattcctgt tggtgcgcat aaagagccac acttccgtct
      301 tcatgctcaa ttgtgacata atttcctgac aaagtatgat acgaagcttc tatgaccgtt
      361 cctgccttgc tcgcatagat tggttctcct tgattggctg ccatgtctaa tcctctatga
      421 aattcttctc ctcgccaacc gaaggaacta gaaattgaat aattttttag aggtaccata
      481 tagccttctt tgcttagttg tggaccagct acttctttat catatttgat taagtcatag
      541 gtttcgatta acccatttaa ttttttatcg tagctagtgt ctgttgcgta tcttcctgtt
      601 aaaaatttag tcgcttcctg ataggttttt gccttacttt tccaaacacc atcataaaaa
      661 gaggtatttc caactaagcc ttcttttaac agtttggcat agtcttttaa gctttcttca
      721 tagctctcat attgccgaaa agttgcttgg gtcgtataaa gtgtcccatt gcctaaatct
      781 tcttgtgtcg caaaagagac gccctttccg ttatgtgtgc ctttgattcc aaatagattg
      841 tagtttggtg cctgagccaa ttgactttgt ccgcttgcgg attctaaaat ggcttgcgcg
      901 atcataacag aagcgtataa atcattttct tgtccaattt tacgagcaga ttcaccgatt
      961 ttacggataa acgtttctac agacgcgtct ttttcaaaat gaatagtccc ttcgttatct
     1021 tgtgtgctgg aagggactaa ttcttgtatc ggtgcgcttg gtgcttggtg gtagtttttt
     1081 gtctcttctg aaccgattga ttgtgcctgc ggtacgtcat tcataggtac agtcacactc
     1141 tgttcaggtg ccttttgtgg gcgttccgtt tttgttggcg gttccgcttc tgtttcggta
     1201 cttggttttt gaggagtctg tgagctttca tcagaaggtt gctcatttgg ttcttctgac
     1261 ggttcctcct caggttgttg attgtcttct ggtttgtctg gttgattcgg tgtttctggt
     1321 tgttcttcag aacctgttgg tggagcgttc atttctggat tatctggtgt atctattgta
     1381 tcttcagtcg gtgtttctgg ttgattttct tctccagtgg aatttgttgt cgtttcttca
     1441 gtgatcgttg cagattgcga atttgtcagt tgattgttgc cttgttcatt agccaaaacc
     1501 gctggcgtat acccaaatac ttgacctact aataacaaag cagataaaca tttactcgtt
     1561 tttttcacaa gattgcctcc attcttcaat caatcatcta gagacagctg attgctgtct
     1621 ctagtcatac tttaattgct ttgtttcatt ttacgacgaa ccagaacagc ccccacacca
     1681 acaaaaagaa acagtcccaa tgcaattaac acatcgttca ctgtttctcc tgtttttggt
     1741 aagctttgtt ttggtgtgct cgtcggcgtt tgtttgacag gggttgtcgg cgtttctttt
     1801 ttaatgcctt ttgctgtcaa tgtttgtttt tcattcttca aatcaaagtg acgtgcttct
     1861 tcctctttat ggtctttatc cttatagaca atatgagttg cgaccaattt ctccccgtct
     1921 tttaacgtgc ctgctttgta gtcaaaggat actttaaact cttcagacgc tttagaaact
     1981 ttacgtgcag tttctaccgt accaacgact gtatcttttc catttttatc aatcttatgg
     2041 aattgagtca cccagtattt ggtttcccct accgtagatt ctggaaattc ttgcgataca
     2101 tgatcgatca actcttggtc tttgtctgga tcaaatgttt ggtcgccatt cactgtaata
     2161 aagcgtgttt cgatttttgg ttctttcgca gtcactgttt gatctttatt ggttaaatca
     2221 aaatgacgag cgaattcttc ttcctcttct ttgtcgttat agacaatgtg cgttgcgaca
     2281 atcgtgtcac cgtctgaaac catgtctgct ttgtagttga acgtcacatc aaatgtttct
     2341 ttttcatcgg tcacttttcg tgtttctttt accgtatcta atacagtgac ttttccatcg
     2401 gcagtaattt tatggaattg cgtcacccaa tatttctctg tcccaaccgc agatttaggg
     2461 aatacttggg acactttatc cactaattct tggtcttttt tcgggttgaa atcttttcca
     2521 cctgtcgttg tcacaaacaa agtagaaatt tctggttggt cttcttctgg aaccacgatt
     2581 tcttcgtttt tcacagtaat tggttgtact tctccgtctt tgaccgttac taaaatcgtt
     2641 tctgtttgtc ctgacgcttg cccataatgc tcagggaaca caatttccgt aaatttatac
     2701 gtaccgtctg gtaattttag tttttctggt tcttcttttg tttcatacac aaaggacaca
     2761 tttttattat gtgcagtcat accttcaatc aggaatttcg ctcctggtaa ttgtagatga
     2821 ttgattgaat cgactttatt gaagtctact tcacccgttt ttaacaagtt gtcaatcaca
     2881 tctaattcaa acgtaagcac aggcgtcgtg ttatcgccat aggccacaga gaacgtggtt
     2941 tgttcttcag aaggttgacg gtagttgtct ggcacatttt tttcaatcaa ttggtatttg
     3001 ttttgatcgc cattgtagaa ttttttgttt gccttccagt gtccgtcttt gtcagttttt
     3061 actgaatcca agacttctcc ttttttcaca aggactgttt ctttatctgg cgcagtaata
     3121 tctgttttgg cgataatatc aaattcgata ttttctgcag gaacggtatc ataaacaggt
     3181 ttgccttgat tccaggattc ttccgcatta tagacttctt tgactttctt tccttctgga
     3241 atgaactgtt gtaagtcatt ttttgcagtt aacgtcactt ctttgtttgg ttcaatcgta
     3301 atcgtatgag tttgtttgtc tagaatatac ccagctggtg ctttgacttc tttgacatcc
     3361 aatttatttt gaacatattt tccgtctttt tcatagaatg cagggatttc ttttaaggcg
     3421 atttcccctt tggcatccgt tgtatattgt tttgtttctc ccgtatcatg acgtttgatt
     3481 tcatacgtca catttggaac ttttttccct gtgacagcgt ctagtttggt tagtttgaag
     3541 tcgccatttt ttagaacttt aatagttagt ccagctctat taggatctgg cacataaagc
     3601 tttcctagat tttgagcaac aggatctaca taggcaaaac ttgaacctac ccctctagaa
     3661 tttggatcca gtgaagaaac agaaattcgt ccgtcatttg agttttgatt tgcttttata
     3721 aacaaactat ttcctgattt tcttacttga acgttagctg tgttttcaac aaccgaccca
     3781 tcccattgag aaaacacacc ttttgtatcc ttaacttcta cttcttcccc tactttaacc
     3841 gtcactgttt ggctatcaaa agaaggatct tcaagtaaat tgtttatttt tctatctaca
     3901 gtctgtttga aagcttcata attagattgt ccaatagttc cataattaac aaatgaacca
     3961 cctaaaactt cccaaatata tgtttgtgct aatacatagt tatccattga accactagaa
     4021 tagtagccaa aataaccaat taaagaaatc ttttttctaa tagcacttgt cgtaattaaa
     4081 ttttcgagtg gtgtcacatc atattgaata cccgcactcg tgattgcgcc ttcttgaatg
     4141 caaaaaacat cttctccgtc aatttgccag cgaaccatgt aatcatgagt atacattgtt
     4201 ccttcaggag taattcgagt catattcaca tctttacgac cattttcagt caatgttaga
     4261 ttggtagcac gtgtctgtgc attcttttcc aagattgctt gccattcttc agattcttgc
     4321 gccaatcgtt cttgttcaga aaaagaagtc gctttttgaa tatcttcaat ggttataaag
     4381 tatttctcat aaccttcagg gacctcttga gtcattacat tgggcggaga aacatcttct
     4441 gtcgtgtcct gcgtagttgt ctcttctgtt gtttcacttg tggtttcagt actgatcgct
     4501 tcttcactac tttttgattc ttcagtcttc attacttctt cagtagaatt tgattcattt
     4561 atcgtttctg caaatgccgt tacagatcct aacatgggat tgagaaatgt aacgaaaatt
     4621 gccaaccaag ttgccattct tttaaacata tttttttcgt tcctttcatt ttatttttca
     4681 gtcttctaaa gtaatttctt atggacttcg acaccgaaac atttttatcc tcctcaaaac
     4741 aaaaaaagac tagaacttat aattctagtc ttcatcaacc acttactttt cttttatttt
     4801 tagtagaaat ctacagtgtg tttttgtgac ccgtcactgt aaataataac atccaccata
     4861 aaacctttgt tgtaccactg cgaatttgga tcgctaacct ctgcattagc ccaatcatag
     4921 gcttcatcta cagtgttaaa tacctttcca ctattaccaa tctctccaac tacaattgtt
     4981 ggctcttcag gtgtcgttgt gccaccattt ccgccttgat cgcctccacc aggattgctt
     5041 ggttcttcag gcgttgttgt tcctccacct gtgcttgggt tagaaggagt tgtttctcct
     5101 ccagtatttc caccattacc tggttgtgta ggtgtttgag tagtgccacc attgttttct
     5161 ggtgtacttg gcgttgttgt accaccagtt gttcctgttt gatctccagc tggtgttgtt
     5221 ggtgtcgttg tatttggtgt cgtttgtgta tttgaagtcg ttccttgtcc tactggttga
     5281 ttcggatcaa ctttgtcttt ctcttgaatc actgtttgag ccacagtttc acctgcgctg
     5341 ttttgtacac ttgctacatt gccattaaat actaatttat ttcctgcata gattaaatcc
     5401 acatttgtaa tcttattcat gtccgctaat ttttggacag taatatttgt tgcttgactg
     5461 attgcgctta gggtatctcc ccagacaatt gtgtattcat tccctttaat ttctgctttg
     5521 atttgttccg ccgaacgagg tgtccaaact ttgttttctg ctgcttcagc attcgttccc
     5581 acagcaagac taactcctcc gactacgatt cctagtgcca atactttcaa cattttcatt
     5641 gtttttcgcc tccgatttga ataaatttat ttacaaaaac atagtaccaa acttatgtct
     5701 atcagtcgat atattttttg tagatttttt tattttttga tatactaact ttggtactaa
     5761 aataactaaa aggaatgtca cacatgcaac ttaataattt aaatgaaata catgattggg
     5821 ctcaaaataa tttacttaat cgtaaacaag ctgccaaaat cactggacaa aactatgatg
     5881 ctttttctag ctctttacgt agaaataata tcaaaccatt tctagaaata ggcgacgaac
     5941 ctggtccttc catcattcgc ttatatctaa gagaagatat tgaaaaatac gcaaaacagc
     6001 tcgaagaaaa aagacagaaa cgtcaatgag gcgttttctg tcttggattt acaagtaaaa
     6061 caataataaa cacttttaca acggaggttc tatgaataag aaatacattg tttcagttgg
     6121 ttcaatcgta ctgattgtac tcgctattat tgctttttct gtatccaaca aaacagaaac
     6181 acagccacaa aatacggttc aatcgactac tacaagtcaa tctagcgatc attccgtcta
     6241 tccttcacta aatagtttaa aagaaacgca tggacaagat ttaattgatc ttggttcttc
     6301 tcaaattact actgtcttat ttgaaaagaa aaatattgcg tttattactc ctaaagaagg
     6361 aactgacctt gaattttcta tcaaagtaac aaataccaaa gaaaatttat tctccattcc
     6421 tgacattcaa tataccccta acaagttaaa gattggggat acgtttggtt tggctaaagt
     6481 aaatgaacaa tactttgctt actcactgaa ataagtttct aatagcaaga ggaaaaaccc
     6541 tcttgttatt tttatcacat aatcatttta taaaaacgct tcgaaaatat caacattttt
     6601 gaagaaacta tttttctaat ttctcaattt cttccaaggg attttcgcta atgacccata
     6661 caggcacaga ttgctctcga ggctcccctt cttctaacca ccaacctgca taatgaactt
     6721 cttcctcctc agcattacca tgataccaac caacaaagga ttcatctaca acgaatgaaa
     6781 tttcttcttc tggatcaaat aattcatttt tccatgcttc aattgcttgt tctttggtgt
     6841 acttttgttt gttaaataca ccacaaattt cgtcatctac ataaaaaatt tcaaaatcaa
     6901 aattactcgt tccttttact ggcatcttaa cttttcctcc tttgactcac ttcaatagat
     6961 ataccactca tgttcattcc attgtttact agttaattgg agttttatct tagggctttg
     7021 tgcactcttt ccaaaaactt gagcgtcgtt tttttcttct aaccaaaaaa ggtaatttct
     7081 tacattcggt actaaatgtt tttcctgcat ttcttcatga aaaacatata attctgcttc
     7141 atttttcaac caattcatta ttcattccct cattcacttc ttcataccgc cattcagata
     7201 gtgagatttc tttttgtttc tccacttgtc ctataaaaat cgctgtattc aacaagctga
     7261 tcacacttaa taataaagca atgaaactaa tggttacact tactctttca cattttgtca
     7321 ttttgtttcc ttctttactc gtttgttatt tcttgtaata ggtctaataa ctccttcacc
     7381 gaaatttgca aatggttcgc aaaaatttca ataaaagcac tcgcacacat taccagacct
     7441 tcaactgata tatcactgcg acaataaaat tctggagaaa cactgatttc tgattctttc
     7501 ttacaattga acgaaaaaac tatttttgaa tcaaattccg gtaattcttc cacaactatg
     7561 ttttgggatt ctaccaagaa agggagtgcc tgttctaatg cttcttctgg tgtttgagca
     7621 gatagaataa ttgcttcttg ttcttttatt tcattgttct caaatacttt ataacttttt
     7681 ttcattgtta gttcctcttt cattcctctc tcaattagct aagccgttac tggtacgcca
     7741 ttctctaaat ttagtatcgc taatccttaa tgctcgtcga atatcaatca ttcgataacc
     7801 tattgcacga agttctttat atcttttagc agttagcgtc tctaacgtca tttcaggcaa
     7861 tgggttcgaa tcaaaaatag aattcgaacc gattatttgg gccaattgtt gtacttgtaa
     7921 cagctcttct gaacttaatt cagtgattgc tcgattgcct atcattgcat acattttctg
     7981 tcgaatatac ttcttttctt gtttagacaa catttctatc cactcctatt ttcattatca
     8041 tttgttacct ccacttcagt aaatccacaa ataaatgaat tcatattcca agtaccattc
     8101 ccttcaacta caacttttct cctgtcctct ttcggatatt caagaatcag accgtttacc
     8161 aatatgtttt tcactactac taatttgttt gtataccgtg gaattgctgt tccgatatac
     8221 ttcactttat ttcctacgct aattgtcatt tcctcactcc tattcttttt tatccaaaac
     8281 tttttcctgt cctattcctg ccaacgaata tacttcactg catgcttaaa caaaacaaaa
     8341 tgttgccctg ttttatttcc gtcttttact tgttccacat acagttcata tttggtcaca
     8401 ccttttaaaa cgccagatac tttttttcat ccattgtcac aattagtttt ttcatcaatc
     8461 gattgatact gcactgctca tttttcttgt tcatatagta ttcctttctt tttcaaaact
     8521 agcctctttt gtttactagt tttcatcaat catcaacgat ttaacagatt ttttctttcg
     8581 cagggtttct agcgtcagca accccaaact ctaaaaaagt gcctttgctc ccttgaaaaa
     8641 cgagcggacg attgtactcg tcatcatatg tttttcatac tacctgatcc acgcaaataa
     8701 cctttttttg ttctgtgcca cacccatcca tacgtatcgc ttgagattca ccatactttt
     8761 ttaggtggtt tatccttcct aagtccctgt tagacacgct gattaactta gaaagtcctg
     8821 tttgtatcca attttcaaag atctcccaaa agaaataagt aggtttacgt gtatcgcttc
     8881 tgttcaaagt acctgtttgt acttgagtat ttctactccc ctcgttcaag ggattccttc
     8941 caccagctag cttttccttt catcatcaaa tgttcctgaa aagcatttac gtttcgtgtc
     9001 cgtgtgctta cttattcttt tttcgatagc gattatccgc tcgctactcg ctattttata
     9061 ctttgtttgt tagctcaaat aaaaactaac aaacaaaaaa ggctagtttc tttccgtttt
     9121 atttttttaa ctgtcgataa atagttgtta gtttaattcc aagttctaaa accaatacgc
     9181 ccaacaaaac cacaggaaaa acaattgggg caaacaagta aaatcggata gttttcatgt
     9241 cttccctctt tctttttggt gtcgctgtgg tcgttcttgc cgttcaatac ttcgatacat
     9301 tttttattca ttatttgctc gttttgtttt cctctggact gcttttcttg ctttttgaat
     9361 actttatacg cttttcgtgc ctgaattaat aggtcttgtc ggcgtttcat tcgtgagaaa
     9421 acaatatttg aaaccatcaa atcctggttc gaacaaccac gattcgcaat aattttccca
     9481 tcaaaaaaaa tagttttcac ttgagaaaaa aacagttgga aatcttgttt tgttgctaat
     9541 ttttttgtca aaatcactgt gcttttccct gttgaatcta caatgattct tcgaacaatc
     9601 cgattcgata acttccaacg ttgccgatta aacaatgtta cttcacaaca aatatgacgt
     9661 aagtgattag gggtcatttt catgtgtcca attcctccaa ccaattatgc atcgaaattt
     9721 tgggcaattg tgacagccca taaatctgcg tttccaggtc ttcataacta caagaacgct
     9781 tggcttgaag taatgaatat tctgcttgat ctgatagttt tgttgccatt ggatctagat
     9841 tcaattctct tacacaacat tcccaaaaat tcaatagcgc tctaaaataa aatccttttc
     9901 gattctgaat tgcttttcct tcatgacgag caattttttc attttgaata caccgttgat
     9961 acgttttgct taaatcttct gcaaatgttt cccctaaaat tcgatactcg cttccatcag
    10021 ataaaccatg tgtttgatta tactttcgtt gccgcttttc tataattcgt ttactgtcgt
    10081 atatcatgtt tagctgctct ttcgcttcac gccattcagc tgaactgcga acaatccatg
    10141 tcaaaacatc tttcggaaca aacgtacttt cgctgtaatg agcaataaac tctttttgcc
    10201 aatcatcagc catttcaaca ggactagtcg tatcgatttt tgtatctaga ttttctgtat
    10261 ctagattgtt agaagaatta ttaaataatt gtgcagaatt ttcacatgga tttttttcgt
    10321 caaaatcatc gaaaatcgct aaattatcaa cagattcctc ctcttctttt ccttgtgcag
    10381 aatctacact ggttgagtca acaggaccta atgaatacac atcattttct gtcgtatcta
    10441 attctcctag atacaaccga gctttgtttc tttttcctgt ttgttgatca aatcccatat
    10501 cctctatttc tagtagctca tattctttca attctttttt gattgtatta acagaacgtc
    10561 tgcaacaatt caatgcattg gctaattctt gttccgtaaa ttcaaaataa acaaaacctt
    10621 gactatctac ttgatttcgt tgaatggcta aatctaagcg tgaccgaaaa accatatacg
    10681 cgatttttgc tttatcagac aatgagcgat atttttcact tgtaataaat acctttggaa
    10741 attgatagta taattcattg tatttacgag ttgcggtaaa tctttttgtt gccatcgtta
    10801 ttacacctca tcttgtccat atattaagaa agaattttgg tgttggtaga cacgaacatc
    10861 ttccattgta gcaatcaatt cttttagata aattcgaccg aattcaattg ttagtaagtc
    10921 gtaatcttct aattcatttt tgatttcttc aatttcttct tcagacacat tgagtaaaat
    10981 ggctaattct tgattggtat aagaaatata gacatgtttc ttttcatcaa tccgtttttc
    11041 aattaaggat tcttctaagt actcattcag taagctatag gcgtattttg cttgtgcagt
    11101 catcgcataa tattttgacg attttataaa gaatagtttt ggcatatgaa aagttgcttg
    11161 tgtcagcaca tctgttgcgc gaaatttgtt ttttaccata ttttttcctc ctagaaaaac
    11221 acgcaaaaaa agctctgtat aaatcgaatt acacagagtt tcttcttgtt gatatttaat
    11281 ttctaaaata cgtttcgggt aaaaactaaa cacttactaa tatttagatt gtagaatctt
    11341 cgccttcatt tattcatttt aaagagaaaa agatcaagtg aaatgtgccc aaaagtttga
    11401 ttctattaat ttttaaacac cattcataat atatttccta taatgtaaaa agcgtcgata
    11461 cttattaaag catcgacgtt atttttatag taaatttatc aaaatttttg ccaacatttt
    11521 ttccgatttt tttccctatt tctat
//
LOCUS       DACCIQ010000074        11488 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCIQ010000074 DACCIQ010000000
VERSION     DACCIQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.076"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(112..1884)
                     /locus_tag="IVM62_002088"
     CDS             complement(112..1884)
                     /locus_tag="IVM62_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846044.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1838..3592)
                     /locus_tag="IVM62_002089"
     CDS             complement(1838..3592)
                     /locus_tag="IVM62_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846045.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3702..4271)
                     /locus_tag="IVM62_002090"
     CDS             complement(3702..4271)
                     /locus_tag="IVM62_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP6846046.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4289..5713)
                     /locus_tag="IVM62_002091"
     CDS             complement(4289..5713)
                     /locus_tag="IVM62_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6846047.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5715..6383)
                     /locus_tag="IVM62_002092"
     CDS             complement(5715..6383)
                     /locus_tag="IVM62_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6846048.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6513..7097)
                     /locus_tag="IVM62_002093"
     CDS             complement(6513..7097)
                     /locus_tag="IVM62_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6846049.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7428..7661)
                     /locus_tag="IVM62_002094"
     CDS             complement(7428..7661)
                     /locus_tag="IVM62_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP6846050.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7676..8626)
                     /locus_tag="IVM62_002095"
     CDS             complement(7676..8626)
                     /locus_tag="IVM62_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6846051.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8623..9465)
                     /locus_tag="IVM62_002096"
     CDS             complement(8623..9465)
                     /locus_tag="IVM62_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6846052.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9467..10186)
                     /locus_tag="IVM62_002097"
     CDS             complement(9467..10186)
                     /locus_tag="IVM62_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6846053.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10878..11036
                     /locus_tag="IVM62_002098"
     CDS             10878..11036
                     /locus_tag="IVM62_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846054.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11108..>11488
                     /locus_tag="IVM62_002099"
     CDS             11108..>11488
                     /locus_tag="IVM62_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6846055.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNIN"
BASE COUNT         3929 a         2057 c         1526 g         3976 t
ORIGIN      
        1 actgattctt tcgtgtattc aaaattagat gctgtttaat aaaattattc tttttgaaaa
       61 agaattataa ctatatccca aaaattgtag gttaatttaa agcctatttc atcatcgtga
      121 ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta tgagattttt
      181 gtatatttct gaccttcgat acaactcatt atgagtacct tgatcaacta gttttccttg
      241 atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg atataattag
      301 tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt cattttccgc
      361 atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca aaaaagcccg
      421 tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt ctccgaccaa
      481 tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta aacgagctgc
      541 ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt ccaaaatact
      601 tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca aagaagctgg
      661 agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat aatcatacag
      721 tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta ctaacgcagt
      781 cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat tgttatcata
      841 tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat ctgatccctc
      901 ttggatactg gtttccttca tagctctaat tcgctgtatt ttaggagcta aataaaatat
      961 ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg cggctagtaa
     1021 gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa cacctactag
     1081 aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga ctgaaaaagt
     1141 acttaattct acctttaaat gaatcctttc actctcttcc atttttttga aaagtctgtc
     1201 ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta tagtttcttg
     1261 aaattcttcc gagttttctc tcaaagtatc gtaatattta gtattggctt tcgtttggga
     1321 tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa cagctaatcc
     1381 cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca gagccatacc
     1441 gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt cagacatgat
     1501 agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta aatttgacaa
     1561 atcgtctgca atttgcactc ttaaatgcgc actttcttta taagtactat tatataagtt
     1621 ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacaaacg atacggcaaa
     1681 ataaagccaa cggggctttg tattttctaa aactaattcg tctatcagca tcattataat
     1741 gaatgctggt accatattaa tgcagtatac aaaaaaacta gaaatagaag ctttagttaa
     1801 agcttctgcg ccctgatcac ttaaagcaaa tttgttttta taaaaacttt tcattgtcaa
     1861 gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact tgatgtttag
     1921 ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag acaagaattt
     1981 catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt ttttctttca
     2041 tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata gatgcactgg
     2101 cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata gctatacgtt
     2161 gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc tctctttcgg
     2221 gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat actttacttg
     2281 ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg aatagtttcg
     2341 tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag tgatttaaag
     2401 gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa attagtttag
     2461 ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt ttttgttcta
     2521 aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg acattttcaa
     2581 atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg tcttgcttca
     2641 tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac aagtgcatac
     2701 ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa aacaacatca
     2761 acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag gttataggaa
     2821 ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa ggtgttttcc
     2881 cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta ttcaaggctt
     2941 taaatgatgt tactttagca ccaaatatct ttataacttg aattccgcgg atatattcta
     3001 ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa tttgtattct
     3061 ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga accaatccaa
     3121 ttttaaagtt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact atggcttgag
     3181 cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg tcatcaattg
     3241 tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct tctgttaaac
     3301 cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata tgagaaaata
     3361 agccagaaaa aatataaaat aaagctccta aagttaacca agcaacagtt tgaaccgcat
     3421 aactcttagc taaatattca tctcctacaa taattagaga ctgaagaaat ttgtaaatac
     3481 tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg gctaaccatc
     3541 cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc attttttcct
     3601 cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc cgtaatagcg
     3661 taaattagag atgtacaaac agataaaagg taaactacgt tctatttttt gtattttttt
     3721 aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac taacactgaa
     3781 atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc agcagattgt
     3841 cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg aatccactgt
     3901 ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat tgctcgatca
     3961 tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga actgataaac
     4021 caaggtaagt acgccccgcc cattaacgta aaaaataaca ataaaacaat tgtgtaaata
     4081 gtaaaaattc cttttctagg gtagttatag ctaatataaa caaatatgat tccagataat
     4141 aatccaaaaa tcccggggga tatgctgtga ccaaacgccc caccaatact actaaccatt
     4201 attgaaaaaa agtaaattac tagggaaata actgtagcta aaccaataaa aatataatct
     4261 tttgttttca tatatattct cctcaattct atttcgttat gtcattaaaa atctgttcta
     4321 tcaaagttga atcttctact ctaagtttta tatctttttt tattccacca ttttttaaat
     4381 aaataattct attgcatgtt ttataaagaa attccggatc atgagtagct ataactactg
     4441 cacccttttc ttttaagtct ttaataaggt cagcaatcct ttccatatta atataatcca
     4501 acccacttgt aggttcatca aatagaaata aattagtttt tgacaatgaa gctatagaaa
     4561 ccaataatct ttgtttttct ccaccggata aatcaaacgg taattgatct ttcattttat
     4621 aaagttccat tttttttaaa attttttctt gctgatcttt actaactatt tcattcccca
     4681 gggctaactc gctcctcact gattctgtga aaaattggta atccatttct tgcattacta
     4741 aaaacggcga tgacaaattt tttttatttt cgattatctt tcctttggta gggcgaataa
     4801 tcttcatgag tattcttagt aaggttgttt tccccacacc gtttttccct accaaaccaa
     4861 taacatctcc aactttgaca tcaaaattta tgtcttttag aatattttca taagtaattt
     4921 cttcaacttt taatacatct gcacttacct tttcgatata gggaacttca atcgaactat
     4981 taaaaatgtc aaatttcctt aatgcgttat tgttttcagt ttttaacaac tgaatagttg
     5041 tagtttttac agttctattt ttaacaaata gaacttgatc aattaattct ttcaaatagt
     5101 aaaaacgatg ttcagctata acaatagtaa atcctttatt ttttaaatca caaagtaagt
     5161 cagcaagttt cttagtcgct ttgaagtcca aatttgaaga cggttcatcc aatataatta
     5221 atttagagtt tagcgataaa ctacatccaa tagaaatttt ctgccgttca ccacttgata
     5281 gcgaaaaaat gtttttatct aatatatttg aaagatctaa taattcagct aattcattga
     5341 ttcttttatc catatgctct tttgtaaatc caaagttttc catcgaaaat actaactcac
     5401 tcgttgtatt tgttgtaaag aattgtcctc taggatcttg acttacgtag ccaatatttt
     5461 ttgatatact ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa atttccccaa
     5521 ccaattttcc gtcataaagt cttggaataa gtccattcat taactttaat agtgtggatt
     5581 tcccactccc agaagaacca gtaatcaatg taagactgcc tttttttatt gaaaaattaa
     5641 ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa agaaacattg ttaaattcaa
     5701 acaattcttt cacctcaaat caaaatactt aagaaaaaaa tagtaacgga aaacaggatg
     5761 atatagtcta ataagctcat gtttgtttta tagtaagaac ttcttttcac aggagattct
     5821 atcccctttg ttattgcatt agcggtcatt tcttcagcaa tcatagttgc acgtataaga
     5881 acgggaacta acagaaattc caaatattgc agaggctgaa aaaaagacaa gcgaatgttt
     5941 ctcattttta aagattcttt aatcattctt atttcagaac gataagtcgg aaagaatctg
     6001 atcactactg taatacttaa cattattttt ttcggtaaat gtattttttc taaagaggat
     6061 aataattcat tgctagtata gacactaacc aacaaccatg agatcattac gaccggcaac
     6121 attcgaaata caatatataa ggatgtcgca aaaaatatag taaaaaactc tatcttgata
     6181 gcatcaataa ttttcattac taaaaacaga actgaataaa tcaagatgaa ataaatacat
     6241 gtctttattt tacgaaacat caatactaaa actgtagcaa taatgaaaca ccccacttga
     6301 aatatatttc cctttcctaa aaaaataatg accggaaaaa aaatatttaa agtaatcaag
     6361 gttaacggat tgatttttga cactctttca cctccaaaaa ataagatcgc actgttatct
     6421 taagctgata aaaatgcacc gttattcaga taacagtgcg atcttatcaa aaagcataac
     6481 tgagaacaat tctcaatgca agcttttttt aattaactca gtaattcaat ccacccagga
     6541 acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc
     6601 ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca
     6661 ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag acaacagact
     6721 gtctttttcg tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct
     6781 gattggaata aaatcaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga
     6841 tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag
     6901 aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg cggctgaata
     6961 atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg
     7021 cctgcggtca ggcacagttg ccttatctta gtttgctgaa atccttcttt ttcaaaatgg
     7081 cttagtccac tttctattaa cttttggtga gttataatat aatctctggt ttctatgggt
     7141 acttcctcct aataagatga attaacttta ttatcttaca caggacttga agtttcaatc
     7201 gttaacgttt attactgaac tcattcctta gagctaaact aatgagaaag agcagaataa
     7261 ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct
     7321 aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat
     7381 aatcactcca atagccattt tcttatcctc cctaaaaata aagtctttta cttaaaacct
     7441 tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc tatatctaaa
     7501 tagtcctcat atctaagttt agccagcatc tcacttacag atgctggact aacacttaga
     7561 acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc ctgaattgct
     7621 agtaaataaa ttagtatagt agtcgtttta atttgtacca tacctaccct ccaaattatt
     7681 tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag taactatcac
     7741 catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata ggaatattgg
     7801 tatcttttga tactgttttc attggtctat catccacact gctttctacg aataaggagg
     7861 gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt gttccttctt
     7921 cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc gagaaatatt
     7981 taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa gcaaatttag
     8041 atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga ttttctttat
     8101 agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt ttagcgtaaa
     8161 taataccgtt ttctaaattt aaccaagcat gaggatcttc cttccctttc tcactctgac
     8221 cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg ttttcctctt
     8281 tgttcgcatt tttaactaat tttgtaaacc aagcatttcc tccagtttcc aagttaacac
     8341 cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc aaaggttcat
     8401 attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta tcttttgcta
     8461 tattttcggt aatatctgct aaaatcgaat tagtaactac tacttttaat ttttcgttcg
     8521 aagccacttt atctttgtct ttactattta ttgaacatgc cccgaatata aaaagagcaa
     8581 tcaaaatccc cagacttccc agtactgctt tccattttct catcataacg atctctcctt
     8641 ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata agaatgccgc
     8701 tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaataa acaatccaag
     8761 caatgatgaa cttgctccga ctgttgcaga aataaatatc atagttttca agctatttgc
     8821 atataaatat gctgtagctg ctggtgtaat taacattgca acaattaata tagttcccac
     8881 actttgcata gctgttactg ctactaaagt taatagaacc atcaatagat aatgataaaa
     8941 actgacattc atccctattg cttttgccat taatggatca aaagaagtga ttaataattc
     9001 tttaaaaaat agaactacta taaccaacac taaaaccgaa acgccaattg taatccactt
     9061 atcaatatct tgtacagcta aaatatttcc aaataaaata tgaaataaat ccgttgagct
     9121 atttgcaact cctataagga taactcctag tgctaaaaaa gaactgaaag taataccaat
     9181 tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa
     9241 tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac
     9301 tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat
     9361 aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg
     9421 taaaaagtga aagtcataca gaccattaat aaaattttga agcatattat tccccctctc
     9481 caataaatat agtatctcca taggcttttt ttaaattttc ttcattaaat acagattcta
     9541 cacttccgtg agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta
     9601 ccttgcttaa atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta
     9661 acgtatccat aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa
     9721 atatgaaatc cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc
     9781 cagataattc tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct
     9841 ttgaaactct ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg
     9901 ttcccataga tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt
     9961 gctctacata tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct
    10021 ctccctgatg agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat
    10081 ttggaccaat aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg
    10141 ctaaaaaatc gttataacaa acattaagct ttttcacttc taacataact ttctccttct
    10201 aaatgataat gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa
    10261 attttatagt ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac
    10321 tacgcgtata gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca
    10381 tgctcctaaa actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt
    10441 gaataagttg ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat
    10501 atacaaatat gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac
    10561 atgtttatcg ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa
    10621 tacaattttt gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct
    10681 gattagcgac aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa
    10741 tagcagcaaa taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc
    10801 tatgtgctta cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta
    10861 tagaatccag agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata
    10921 atactctaga taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag
    10981 ttcgctttga cgtagaagat attaaagact ttatgaatac atttaaaata acttgaaatt
    11041 ttcttctcgc cgcatatact aaaattagtg aaatatactg ccttactttt tttaaggagt
    11101 gaggattttg cctacaaagc tttcaaatgg gaaatataaa actaatctga gatatccaaa
    11161 aaggtttaaa gaaataacag gaattgcatc agaaaagtat caaaaaacat ttccaaatag
    11221 gcaattggcg attaaagccg aaaatgatat gaagaagaaa atagaaaaag ttttgagaga
    11281 ggagaatgca aactcattag aattaaaagg aaaaataacg tttaaaaaat tttacgaatc
    11341 taaatggctt cctagatatg aattaggtca aacaattaga tcaaacaggc ctcctagtga
    11401 tataaccatt agtaatacaa aagatatatt tcggttgcat attttaccta tgtttggtga
    11461 atacgcaatg aattatctaa atatcaat
//
LOCUS       DACCIQ010000075        11222 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCIQ010000075 DACCIQ010000000
VERSION     DACCIQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.077"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(572..937)
                     /locus_tag="IVM62_002100"
     CDS             complement(572..937)
                     /locus_tag="IVM62_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846056.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1013..1258)
                     /locus_tag="IVM62_002101"
     CDS             complement(1013..1258)
                     /locus_tag="IVM62_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6846057.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1328..1582)
                     /locus_tag="IVM62_002102"
     CDS             complement(1328..1582)
                     /locus_tag="IVM62_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846058.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1746..2069)
                     /locus_tag="IVM62_002103"
     CDS             complement(1746..2069)
                     /locus_tag="IVM62_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846059.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2256..2329
                     /locus_tag="IVM62_002104"
     tRNA            2256..2329
                     /locus_tag="IVM62_002104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2290..2292,aa:Ile,seq:cat)
     gene            complement(2714..3211)
                     /locus_tag="IVM62_002105"
     CDS             complement(2714..3211)
                     /locus_tag="IVM62_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6846060.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3251..4060)
                     /locus_tag="IVM62_002106"
     CDS             complement(3251..4060)
                     /locus_tag="IVM62_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6846061.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4394..5251
                     /locus_tag="IVM62_002107"
     CDS             4394..5251
                     /locus_tag="IVM62_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6846062.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5244..6146
                     /locus_tag="IVM62_002108"
     CDS             5244..6146
                     /locus_tag="IVM62_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6846063.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6179..6529
                     /locus_tag="IVM62_002109"
     CDS             6179..6529
                     /locus_tag="IVM62_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6846064.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6563..7501
                     /locus_tag="IVM62_002110"
     CDS             6563..7501
                     /locus_tag="IVM62_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6846065.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7579..8472
                     /locus_tag="IVM62_002111"
     CDS             7579..8472
                     /locus_tag="IVM62_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6846066.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8510..9073
                     /gene="amaP"
                     /locus_tag="IVM62_002112"
     CDS             8510..9073
                     /gene="amaP"
                     /locus_tag="IVM62_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6846067.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9087..9275
                     /locus_tag="IVM62_002113"
     CDS             9087..9275
                     /locus_tag="IVM62_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6846068.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9288..10190
                     /locus_tag="IVM62_002114"
     CDS             9288..10190
                     /locus_tag="IVM62_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6846069.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKNGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10222..10464
                     /locus_tag="IVM62_002115"
     CDS             10222..10464
                     /locus_tag="IVM62_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6846070.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10720..11091)
                     /locus_tag="IVM62_002116"
     CDS             complement(10720..11091)
                     /locus_tag="IVM62_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846071.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3973 a         1923 c         2130 g         3196 t
ORIGIN      
        1 agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc gttattattt tagtctattt
       61 tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa cgctagcaat
      121 ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa ctgaagccac
      181 gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa atcgacgatg
      241 catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa gaaggtgtag
      301 aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct gtgcatcgta
      361 tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg agatgttcat ttatgtctag
      421 ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg aggatgaaga
      481 taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt tttaatgaag
      541 taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct ttttgctcca
      601 aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc ggcacgttgc
      661 gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac ataggatttt
      721 tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca agctgatgat
      781 attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca tgtttctttc
      841 cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc tgttgacgag
      901 gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct ttatatatag
      961 attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa aatcacttca
     1021 gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg taaaagtcag
     1081 cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac ttacgatatt
     1141 tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat cggccagttc
     1201 ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg atactcattt
     1261 aatcaccttt cattatagta gaatagttat aatgactata gaaagctaca catgcaatca
     1321 atagtactca atgtttttta tttaaacgta tcaaataaag aatgacgatc aataaagaca
     1381 aaaccagtcc tgataattct tctataaaaa cactatcggg ttcaaagtag ttgtaattaa
     1441 aaactttcca cataaagtag atagccggaa gagtcaaaat agctaaataa aaccgttctt
     1501 tgttccaatt cataagataa acaatacttg cgaccagtgc gaccgcaggc acccaaaaca
     1561 aaactctcac caacgtaatc atgtaacgga ctcctcaacg attcatataa taaaagataa
     1621 catgttattg catacatcac aaatgaaatg ctaggaaaat aaaagaagaa tagatcaatg
     1681 aaaaatgaaa aagcagaaca gttttctgct gataagcagg aactgttccg ctgaatgtgt
     1741 cttatctatc gagaaaaaat caatggattg atatctaagt aattcaatac atataaaaag
     1801 aggacgatcg cattcactaa aaaggaaaga atgatgatcc catagcgaat ggaaacatat
     1861 ttaacaaata cttttgtttg aatacgggca tatctttttc ctttatagct gagcatcatg
     1921 acaagaagca aaagggtaat cacaccttga aaaatcattc caataaacag aattcctgag
     1981 gtttgataat ttaattggag aaaataagct gctgccgcaa ctaaatcgaa taaaatcgca
     2041 ataaatggca aaataatcac tcctaacatt gagaataaat aaaagaataa cattttttct
     2101 aaaataagca aataaaaatt aaagtaacaa aatagttatg acacaagtaa aagaaataag
     2161 aataacaata ttattttact tatattttga aacttgtgag agtttgtcat tcgtgctata
     2221 gttgaaaagt aacaaaaata aattttattt atgatgtacc tttagctcag ttggttagag
     2281 cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta caaggtacat aagaggttgt
     2341 gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
     2401 ttacttggct gttcttatta ctgcagatgc aagcttttga ataccgttta aggaagaggt
     2461 tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa cggaaaaata gttcctcata
     2521 ttttgccgtt tgttaagcta ataccgaggg tcagctgatt gaacaccatt taagaaagag
     2581 gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc aatctacttt gatagaaaaa
     2641 gcacgtagga ctaaaacagt tctgcgtgct ttttttattg tgtgttaaat atcgtaaatg
     2701 aagatagata tttttatgat tcgacgatag agacgttgaa atctttcaac agctctaaag
     2761 cggattctcc gtgttttcta ctggagacta acattggatc aacaaaaaag tctgcttcag
     2821 gaaaagcact ctgcaggcct atcagattag agagtacaca caagtttgtt tcaatcccga
     2881 ctacttcgat ctctttttgt tcttctttgc tttcaaataa agtttcctgg atttcagcta
     2941 aagtttcagg tggaatcgta aagtaatact ttttaatcac tcgttcattt tcttgtggag
     3001 aaagaagagg gatcaattgc aaatcttctc gttcttcttc tttgtccttc agttcaactg
     3061 gtatatcccg tgtgaagaga atatattcat tattttggcg tgctttagct aatctttgag
     3121 caatacgttc tgccaattga tctgactctg gaatataaaa ttcgctgttt ggatcaagaa
     3181 tatgattttg catatcaata ataactaaca ttttctttcc tcctacattc atatcgtcat
     3241 tttattcttt ttaatagaag ttttttaaaa tataagcaac accagcttct tcattcgtca
     3301 gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc attccccatc gcaatactca
     3361 acccgctgta attcaacata gcaatgtcgt tttcttgatc gccaaaagcc atgatgtcac
     3421 tcgattcgat tccaaggtaa tccgccagaa aaacaagtga atgagcttta gtgatacctg
     3481 taggcgtgat ttctaagaaa tgcggatcag agaatactga ttggatacca tctgcttgca
     3541 gatctctttg tagcttctgt agtttttctt cactcttatc tgttaatgct aatttaagat
     3601 agctaagaga aggatcttcc aacatattta aaatttgatc aaaagaatgg ttctgtaaat
     3661 tgaaatgttc ataataaggg tcatctttga ctagatttga ttcatcgaat gtcagctgat
     3721 catcttgggt ttctcggcta gcaatgatgg atattttcgc ttcttgagca tagaagaagg
     3781 cttttctcag tgtatcgcta tctatttttg aacgtctgag aactttcgcc atttttccat
     3841 ccttcatttc agcaatcagt gaaccattgg aagaaatcaa atagccaccg attcctaatt
     3901 cggaaaaaat atctttagca gaaatgctgt tccttccggt agcgacggtt acgattccgc
     3961 cattttcatc aaatttccga agtgcttgga tattttcttc tggtatcttt ccatttcgat
     4021 caagcaaggt accgtctaaa tcaatcgctg ctagtttcat tttatcttcc tactttcttt
     4081 ttacatatct attttcctgt taccttaaga ttaaatgaga atttaaaaaa atgaaactaa
     4141 cttgctttgt cattatgaat tttttcaaaa aacattctta agaaaaaggt ggctatatat
     4201 agtagttttt ggtcaatacc tttgaataaa aagccatgaa aaacgaatag ttagcgctta
     4261 ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat gaaaacaaag catatatttt
     4321 gctttaaaac caaatgagga atattctaga tgtagaacag gttagaaaaa gattagaaaa
     4381 aggagtgatt ttgatggaga agagatacgg cgtagttcct gtggtaactg cttcagacga
     4441 aaattatgct ccttacttga gcgtgatgat cgcaactgca ttagaaaatt gcaataaaac
     4501 aagacgcata aaattttatg tgattgacga tggtctatca gaatatagta aagaaggatt
     4561 ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc caattcctga cagtggaaaa
     4621 agatatctac gaagacttcc tagtcagtga ccatatcaca actaccgctt acttaagaat
     4681 ttctttacca aacttattag ctaaagagga ttataagaaa gtcctttact tagattcaga
     4741 tgtgttagta ctagacgata tcgtaaaact atatgatgag ccgttaaatg gaaaaacaat
     4801 cggcgctatt atcgatccag gccaagtgaa agctttggaa agattaggaa tcgattccga
     4861 tgacttgtac ttcaattcag gagtgatggt gattgacatt gatcaatgga ataaaaaaga
     4921 aattacagaa aaaactatcc attacctcag tgaaaatggc gatcgaatca tctatcacga
     4981 ccaagatgct ttgaatgctg tactttatga agattgggaa caacttcatc caaaatggaa
     5041 catgcagact tcgctgattt ttgaacgtca tccagcacca aatgaaaaat atgaacgtca
     5101 gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt acaggacatg ataaaccttg
     5161 gaatacgttg aaagatcatc cgtataccaa tttatactta aaaaaattag cacacagtac
     5221 tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat agcagtagtc gcaagttgta
     5281 acacaaaatt cgtaccacat ttagctgcat tatttgtctc agtattagat aattgcaacc
     5341 cgtctaaatt tgttcggttc tatgtgattg acgatgatat cgattttgaa agtaaacagc
     5401 ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga tgtggaattc ttgaaaatca
     5461 ataaagaatt ctttacaaat gtggtaatca gcgatcgtat tccagaaaca gcttattatc
     5521 gtattgcgat tccggaattg ttccgcggca cagaagttga acgtatctta tatatggatt
     5581 gcgacatgat cgctttacaa gatatctcta aattatggcg tcttgacttc ggtgattcaa
     5641 tcgtagcagc tgttgaagac gctgggttcc atcaacgact ggaaaaaatg aaaatttcag
     5701 ctaaatcaat gcgttatttc aactcaggtt tgatgctgat caatgtgaaa aaatggttag
     5761 atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca taatccagaa aaattacgat
     5821 tccatgacca agatgcatta aatgctatct tacatgaccg ttggttacca cttcatccgc
     5881 gttggaatgc acaaggctat atcatggcaa aagcaaaaaa acatccaaca gcagcaggtg
     5941 aacgtgaata cgaagaaact cgcaacaatc cttacattat ccatttttca ggacatgtga
     6001 aaccgtggag caaggatttt gaagggccaa cgaagaaata ttacgaaaaa tatgcaggga
     6061 tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc aaaatatgca aaagttcaac
     6121 caaaaacaga tgtagttgtt aaataataga agacaacaac tgaagagggg gagtaggaat
     6181 ggaaattaca atgtctaaaa atgcagtaga gacgttaatt gaaaaggttg gggaaaatac
     6241 aaaaatcgca ctcgcgctta tcaatgattc cgatccattt ctaagggaca aaggtgcttt
     6301 tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg gaatttggag aatatgcgac
     6361 taaaatcgag catccattat tggacatcta tacatctaaa ttagagcaaa attatttcgg
     6421 taaacgattg aacatggact tcaacaaaca gcttgacagc ttttcattag aaaatgaaat
     6481 cgcagtactc gattataata tcaaattaaa gaactgcttc ttcagctaat agtttcgaga
     6541 aagtccaaaa aggaggtata cattgagata ctactttgtg gatgaagaaa agaatgtaat
     6601 agaaacagat gaaaaaaaat ataattggct cgtacttgat agtgcaaagc ccgaagagat
     6661 tgagcaagca atcgaagaat tcgagcttcc tgatgatatt ttcgtaggaa ccgaatatcc
     6721 ggaagaagtt tctcggatgg agcgccttta tgagacgaaa ctagagcatc cattttcttt
     6781 ggtagtgatt aatctagatc agtcagataa tcgtatcgaa aaaaaattaa cgccaatatc
     6841 ttttgtactt tccgataatc tcttgattac ttgtatcgat agaaaaaccg aatttgtgga
     6901 tcggctgctg acaaagcatg gaaatcattt aaactcgttt gaaaagatca tcacgtatac
     6961 attattagat atttacaccc attacgtgaa agaactaaga gagatgaaaa agcgaattga
     7021 cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa gaactgtata aacaggcaga
     7081 tcttgaaaga gacatcgtat atatcgatca taccttaaga gatcaacggg aaacaatgga
     7141 tcaattatgg aattctgaaa ctttcatcaa acgtttagac gatgaacagc tactctatga
     7201 tgtccatttg agacagcgac aaacagaaaa aatgatcgag atctatcgag atttattaga
     7261 gagtattggt gatctattca acgggatgat ggacaataat ctaaatcata tcatgaaata
     7321 tctagattca gctgcattaa tcatttccgt tccggcgttg ttcgcaggga tttggggaat
     7381 gaatacagga ggattaccgg gtaaaagttc tgctctaggc tttatactcg tagtctttgg
     7441 ctctttgata gcagcagtag ttttaggtta ccatttatac cggaaagatt atacaaatta
     7501 aagaatctat ttaaaacagg tatcaaacaa agaaatttgt tttggcaata caatttaaaa
     7561 actaggagga atacagcaat gacagaacca aaattaatcg acccgcgaaa cttgtaccat
     7621 acggagaaat ttcctaaaca agatcaggat acaccagctt tacagcagaa tatgattcct
     7681 gttcctgatt gtggagaaga atcctatgaa ggcaataatc aactagaaaa ccgccgtgta
     7741 ctaatcactg gtggagattc agggatcggt cgtgcagcag cgattgcatt tgctagagaa
     7801 ggggcagata tcgctcttca attcttccca ggagaagaaa aagatgcaga ggaagtagca
     7861 aaatacgtga aagttgcagg acgtaagatt gtcttactac cttttgatct tcgtgacgaa
     7921 caagcaccta aagaaatcat agataaagcc gtgagtgaac ttggcgggct agatacgatg
     7981 gtactaaacg caggacagca aatttcttgt cagtctattg ctgagttacc acttgagcaa
     8041 gtcaaagaca catttatgat aaacatcatc gctatgtttg ctttagtgaa agaagctgtt
     8101 ccgcatatcc cagcaggagg agcaatcgta acaacaactt ctgttcaagc attcaatcca
     8161 agcgaacatt tattagatta cgcagcaaca aaagcttcaa tcgctaactt tacgatcggt
     8221 ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg gtgtagcacc tggtccaatc
     8281 tggacaccat tacaactaga cgaaggacaa ccttctgatt cgatccctga atttggtcag
     8341 caaagtttac tagaacgtgc aggtcagcca ggagaattag caccagttta tgttttctta
     8401 gcatcgaata aagcaagtta tgttacagcc caaatttacg gagtaactgg tggagaagcg
     8461 atcaacttat aagaagttga tcgtttcacc cctatgagga ggtgcgaaaa tgaataaagg
     8521 tgtaaaaacg atcggagtga tcggaacatt acttttgcta tctgtgcttt tgtttgcgat
     8581 actattaagt gcaacctatc cattgccttt tcagctagag aagtttcgat tcttttccat
     8641 cactaattca tacgtgcaac aatatgtatt ttggcttgcc gttgcatttg ctattttgac
     8701 aattatcacg ttacttgttt tcctgtttta tccaaaaaca atcggaaact ttctactgaa
     8761 acatggtgat ggggagttga cgttggataa aaaagcaatt gaaggaatgg ttcgttctca
     8821 cttgcatgaa gatgaattca tccattcacc aaaagtaaat gtcaaagcaa cgaaaaacaa
     8881 gatcgccgtc aatgtaaaag gggagttgaa acgaacttca gcactcattg gtaaaacagg
     8941 aacattgatg aaagaaatcg aaaatgaagt acaacacata ttgggaacaa aagagccagt
     9001 gaaagtatcg gtgaaataca caggctttga agaaccagct aaacgaagca atgaccgcaa
     9061 acgagtagaa taggggggga ccaaaaatga aagaactatt ttcgacatac aagctcccta
     9121 tcatctttgg ggtacttgga ttgatattag ccgttttatg gatcaccgtg ggctttttca
     9181 agaccttatt gatcttgatc atggcagcga tcggcgtcgc tatcggtttt tatctgaaac
     9241 aaacgaaaat cttagatcac tattttaaat cttaggagga aacatccatg aataacaata
     9301 acaaaaataa taacaataaa atgaacaacc aaaaaccagt gtctgcacat ccagacccaa
     9361 cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa caaaacagca ccagtatctg
     9421 cacatccaga tccagtagtc cctaacgttc aacaaggagt agaagagaag aaacatgatg
     9481 cagaaaagca tgcacctcat catccaggaa ctccagaatc taaagttcca gaacaacatg
     9541 atgcaatcga tccttctaaa cctgtggaag agcaaaaaca tccttctgac catgcgaaag
     9601 cacaagaaaa acttagttct gaccacacaa acggacaagg aaaacttggt tctgatcata
     9661 caaataacca aggaaagcat gattctgaac aggaagattc aatgcgcggt gagctgaatt
     9721 atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat tgaacaagta gacggtttat
     9781 tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa acttgtgaat acagataatg
     9841 taacggctgg gatcgaaaca gaagtcggta aaaaacaagt agcagttgat atggatgtca
     9901 ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga aatgcaagaa gtaatcggca
     9961 aagaagtaaa aaatatgaca catttagaag tgatcgaagt caacgctaac gtagtggaca
    10021 tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt gcaagacaaa gtaactgacg
    10081 cagcgaaaaa aaacggtgaa tttgcttcga atcaaacaga taaagtaaaa tcagcagctg
    10141 gaagcggcgc acaaaaagta aaagaaaata cagaaccacg ggttcagtaa taaggaagta
    10201 aaacattagg aggaacgaaa catgggattt atttggtcac ttatcgtcgg aggaattatc
    10261 ggagcaattg caggagcaat cacgaataaa ggttcttcga tggggattat tgcaaatgtc
    10321 gtagcaggtt tagttggatc agcacttggt caagcattat taggttcttg gggaccaagt
    10381 attgcaggaa tggcaatcgt gccatcattg attggtgcca tcattttagt actaatcgta
    10441 tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag aggtcgtgaa aaagctgttc
    10501 ttcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc atttggttgg
    10561 gcatattatc gaagatgcaa gcttttgaac actgtttata cggaataaga ggctgtgaca
    10621 agacttttgt cacagcctct tatttatgcg ggcaaattcc caattagatt tttaaaagtt
    10681 tttccttaaa tgtgtcttta ttgatacaga gaagcttctt taataaaaca atgcctccct
    10741 aaattttctt gagataggaa aggtgaacat acaaaataat acgaccactt tgaaaaccca
    10801 gatgatccac aaatggatcg tatgatactg atgaagtaaa aagcaatgga tcagaaactc
    10861 aaagagattg aaaaagacaa ggaagaaata atatctcttt tttactcttg tctgttttag
    10921 ccaataatag ctcgtaaagt aatctagaca aacaaataga agtaaacaaa gtttcgtaag
    10981 agcagcaatc gtatcatgtt gctctgctag acgattgacg agcaggaaaa atgttggtaa
    11041 tggaatatag atcacgagta atgaaagaaa aaagtaaaca ataaatggca tactattcct
    11101 cctaattgtg tgagttcgcc ctcattttaa aataacgata tttgtttgtg aagctttttc
    11161 gtgtcgtaac aaatgttatc taatgtattt ttaaataaat aatcattatt atcaaaataa
    11221 tg
//
LOCUS       DACCIQ010000076        11169 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCIQ010000076 DACCIQ010000000
VERSION     DACCIQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.078"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            131..1390
                     /gene="lysA"
                     /locus_tag="IVM62_002117"
     CDS             131..1390
                     /gene="lysA"
                     /locus_tag="IVM62_002117"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP6846072.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1377..2177
                     /gene="dapF"
                     /locus_tag="IVM62_002118"
     CDS             1377..2177
                     /gene="dapF"
                     /locus_tag="IVM62_002118"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP6846073.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2180..3061
                     /locus_tag="IVM62_002119"
     CDS             2180..3061
                     /locus_tag="IVM62_002119"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP6846074.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3058..3774
                     /locus_tag="IVM62_002120"
     CDS             3058..3774
                     /locus_tag="IVM62_002120"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP6846075.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3780..4475
                     /gene="dapD"
                     /locus_tag="IVM62_002121"
     CDS             3780..4475
                     /gene="dapD"
                     /locus_tag="IVM62_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP6846076.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4491..5492
                     /locus_tag="IVM62_002122"
     CDS             4491..5492
                     /locus_tag="IVM62_002122"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP6846077.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5489..6850
                     /locus_tag="IVM62_002123"
     CDS             5489..6850
                     /locus_tag="IVM62_002123"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP6846078.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6810..7961
                     /locus_tag="IVM62_002124"
     CDS             6810..7961
                     /locus_tag="IVM62_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP6846079.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7936..9105
                     /locus_tag="IVM62_002125"
     CDS             7936..9105
                     /locus_tag="IVM62_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6846080.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9280..10659
                     /locus_tag="IVM62_002126"
     CDS             9280..10659
                     /locus_tag="IVM62_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6846081.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10940..11169
                     /locus_tag="IVM62_002127"
                     /pseudo
     CDS             10940..11169
                     /locus_tag="IVM62_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3723 a         1950 c         2498 g         2998 t
ORIGIN      
        1 ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca
       61 ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag
      121 gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa
      181 gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt
      241 caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt
      301 ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag
      361 tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca
      421 tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat
      481 tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa
      541 aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat
      601 cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca
      661 agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat
      721 cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta
      781 cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta
      841 tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac
      901 ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag
      961 aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc
     1021 acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta
     1081 tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt
     1141 gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc
     1201 gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat
     1261 gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc
     1321 aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga
     1381 tccttcataa atataatggc tgcggaaatg attttatctt attagattac gcagaagaca
     1441 cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat acagacggat
     1501 tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg
     1561 cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga
     1621 tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg
     1681 atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa
     1741 tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag
     1801 taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc
     1861 gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa
     1921 aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg
     1981 aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg
     2041 ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact
     2101 tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa
     2161 gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa
     2221 tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga
     2281 acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc
     2341 tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat
     2401 tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc
     2461 tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg
     2521 actagctgct cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa
     2581 tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca
     2641 tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc
     2701 tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt
     2761 aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt
     2821 ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt
     2881 aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc
     2941 agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc
     3001 aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg
     3061 aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa
     3121 agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt
     3181 acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt
     3241 tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca
     3301 cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt
     3361 tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc
     3421 tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact
     3481 gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg
     3541 caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta
     3601 cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa
     3661 tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat
     3721 cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg
     3781 tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag
     3841 tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa
     3901 gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag
     3961 aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag
     4021 atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa
     4081 tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg
     4141 aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact
     4201 gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg
     4261 ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata
     4321 tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa
     4381 ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa
     4441 atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa
     4501 tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag
     4561 aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta
     4621 aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg
     4681 aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa
     4741 tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg
     4801 atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg
     4861 caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct
     4921 ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag
     4981 gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata
     5041 ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag
     5101 aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag
     5161 caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc
     5221 aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat
     5281 tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata
     5341 tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata
     5401 cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt
     5461 taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg
     5521 cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga
     5581 tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa
     5641 agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc
     5701 cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt
     5761 acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga
     5821 agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg
     5881 ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca
     5941 ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc
     6001 agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga
     6061 cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga
     6121 tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga
     6181 aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc
     6241 agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc
     6301 tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg
     6361 aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga
     6421 aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca
     6481 tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa
     6541 gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat
     6601 cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc
     6661 tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg
     6721 agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa
     6781 gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc
     6841 aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa
     6901 aaaacagcgg ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata
     6961 atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag
     7021 aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg
     7081 gacatatggc gatgctttta gggttgactg actttataac taaacacatt gaggatctta
     7141 tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg
     7201 ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat
     7261 ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca
     7321 gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa
     7381 tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat
     7441 taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa
     7501 atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc
     7561 aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta
     7621 tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag
     7681 cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt
     7741 ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc
     7801 ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat
     7861 ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag
     7921 aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata
     7981 gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa
     8041 ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca
     8101 gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa
     8161 gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt
     8221 ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa
     8281 ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta
     8341 gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa
     8401 caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat
     8461 cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt
     8521 ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt
     8581 ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta
     8641 tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt
     8701 attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa
     8761 aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa
     8821 tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc
     8881 cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct
     8941 tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca
     9001 tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta
     9061 catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc
     9121 aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt
     9181 agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt
     9241 tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc
     9301 attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt
     9361 gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt
     9421 atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca
     9481 gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat
     9541 gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct
     9601 tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca
     9661 gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa
     9721 ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa
     9781 gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga
     9841 cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa
     9901 aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt
     9961 gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt
    10021 gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc
    10081 caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca
    10141 cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt
    10201 agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc
    10261 ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg
    10321 aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg
    10381 ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc
    10441 tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca
    10501 ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca
    10561 gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc
    10621 gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag
    10681 aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct
    10741 tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10801 ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat
    10861 ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga
    10921 tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat
    10981 tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac
    11041 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa
    11101 gaagctagga ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg
    11161 aggaaaagt
//
LOCUS       DACCIQ010000077        11168 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCIQ010000077 DACCIQ010000000
VERSION     DACCIQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.079"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            133..504
                     /locus_tag="IVM62_002128"
     CDS             133..504
                     /locus_tag="IVM62_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846082.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            589..789
                     /locus_tag="IVM62_002129"
     CDS             589..789
                     /locus_tag="IVM62_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846083.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            923..2149
                     /locus_tag="IVM62_002130"
     CDS             923..2149
                     /locus_tag="IVM62_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846084.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2999..4323
                     /locus_tag="IVM62_002131"
                     /pseudo
     CDS             2999..4323
                     /locus_tag="IVM62_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4316..4663
                     /locus_tag="IVM62_002132"
     CDS             4316..4663
                     /locus_tag="IVM62_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846085.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5014..5835
                     /locus_tag="IVM62_002133"
     CDS             5014..5835
                     /locus_tag="IVM62_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6846086.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            5855..6415
                     /locus_tag="IVM62_002134"
     CDS             5855..6415
                     /locus_tag="IVM62_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP6846087.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            7388..8155
                     /locus_tag="IVM62_002135"
     CDS             7388..8155
                     /locus_tag="IVM62_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP6846088.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            8181..8726
                     /locus_tag="IVM62_002136"
     CDS             8181..8726
                     /locus_tag="IVM62_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846089.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            8727..9293
                     /locus_tag="IVM62_002137"
     CDS             8727..9293
                     /locus_tag="IVM62_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846090.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            9308..9556
                     /locus_tag="IVM62_002138"
     CDS             9308..9556
                     /locus_tag="IVM62_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846091.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            9575..9814
                     /locus_tag="IVM62_002139"
     CDS             9575..9814
                     /locus_tag="IVM62_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846092.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            9831..10415
                     /locus_tag="IVM62_002140"
     CDS             9831..10415
                     /locus_tag="IVM62_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846093.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            10522..10803
                     /locus_tag="IVM62_002141"
     CDS             10522..10803
                     /locus_tag="IVM62_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP6846094.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            10793..11134
                     /locus_tag="IVM62_002142"
     CDS             10793..11134
                     /locus_tag="IVM62_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6846095.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         4247 a         1420 c         2210 g         3291 t
ORIGIN      
        1 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
       61 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
      121 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
      181 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
      241 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
      301 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
      361 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
      421 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
      481 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
      541 cgtattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
      601 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
      661 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
      721 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
      781 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
      841 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
      901 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
      961 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     1021 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     1081 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     1141 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     1201 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     1261 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     1321 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     1381 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     1441 aagaacgcct agaatatata aaaatagaaa caaatatgga ttactacctt ttagaggtcg
     1501 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     1561 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     1621 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     1681 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     1741 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     1801 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     1861 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     1921 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     1981 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     2041 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     2101 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     2161 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     2221 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     2281 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     2341 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     2401 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     2461 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaatagcta
     2521 atcgaagtaa aacccaaagc catatgaacc cctgaatcag cgaaagttta tgatgatttg
     2581 gattactaat agtttaggaa ttagatggta aatctaaatt aaacaatcgt tgatttggca
     2641 acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt tatgtgacca
     2701 gacggaagga cgaatgttgt tgaggtttta gaaccatata aaacagcata gttctcaaat
     2761 tgtttaaaat gattgtcaag ttaaaaaaat ctgggatcat ataaaatagt atagttctca
     2821 aatatgatta gaaaccctat taaagagcaa tattaagcca acttacttaa atcgtttaat
     2881 taagaacaat aatctctttt agtcagactt ttagtctgac ttttttgtat ttccaaaatt
     2941 tattctttac aaaaacgaac atacgtttgt ataattgctt gtgaatgggg gaataaaaat
     3001 gtttgattac agtagagaac cggtgaatga ctatttttta atcgacatga agtctttcta
     3061 tgctagcgtg gaatgtattg aacgaaattt agacccaatg actacaccgt taattgtcat
     3121 gagtagatcg gacaacactg gttcaggtct gactctagct tcttcaccta ctgcaaagaa
     3181 aaaatatcat attactaatg ttagtagagc tactgattta ccttatccat taccaaaaga
     3241 acttcatatt gttccaccac gaatgaactt atatataaaa aaaatatgga agttaatcag
     3301 atttttagaa agtatgtcgc agatgaagat ttattgattt attcgattga tgaatcaatt
     3361 ttaaaagtga caaaatcctt aaatttattt actagttcag gaacaagaag cgagagaaga
     3421 agagaattag ctaaaataat caaagatgag attcagaaag aaacaggtct aatagcaaca
     3481 gtcggaatag gagataaccc attattagct aaacttgcac tggacaatga agcgaagagt
     3541 agtaaagaat ttatagctga gtggacttat gagactgtac ctgaaaaaat atggaaaata
     3601 aacgagatga ctgacttttg gggcattgga tcacgcatga aaaaaagatt gaatcatatg
     3661 ggaattatga gtatagaaga tttagcaaat tggaatccct acacaattaa aagtcagtta
     3721 ggtgtaatcg gactccagtt atatttccat accaatggaa ttgatcgtac ggatattgct
     3781 attccaccag aaccaaccaa agaaaagtct tatgggaatt cacaagttct accaaaagat
     3841 tatacgagga gagaagaagt agaactagta gtcaaggaga tggcagagca agtagccatt
     3901 cgaattagac aacacaattg caaaactggt tgtgtacgct tagcaattgg tacgtcaatt
     3961 ttggaagaaa aatcaggttt ctcgcaccag atgaaaatac caattacaga taatacgaaa
     4021 gaattacaaa attattgtct ttttttattc aataagtttt atgaggggca ggaagtacga
     4081 catataggag taacttatag cagattagta tacacagatt ccctacaatt agatttattt
     4141 agtgatccaa gaaaaaagat tgctgaagaa aatttggata ggattattga taagattaga
     4201 caaaaatatg gatttacagc tattatacat gcttcatcta tgttagaagg tgcaagaagt
     4261 attacgagaa gcactttaat aggaggacac gcaggaggat taggaggttt gcataatgat
     4321 tgaatataac gatagaggac gtttaaaatg gtcgggattt tatttgtctg atcatactga
     4381 aagaatcaat gcagacaatg tacaacgtgc taatcataat ttggcaaaag aacaaatgac
     4441 gactgaagag attgcgaaaa tactgagaac tgctgtcata aaaaatagac ccgtctgcat
     4501 tcaaaaagag gaacgaaacg ctgaaggtta ctatccagat gatgtagtag gaaaaattat
     4561 tggatatgac gagcttggtg tatttgtgga agaagaaaaa gttcactatg atgaaattag
     4621 gaatgtttct ttctcggaat tattaaaatg gagtcagata tgaacattat taaaaaatac
     4681 gaattcggtt atattacgta tgaacagcta atagaggaat tatggggata tggtcaacaa
     4741 ttaattaatg aagtggggat tgattgcttt tgtttctata ttgaatccgg agcaggatac
     4801 cataagaaca gatactatat atttccatat ccaccaaaat agtctcttag atatttttaa
     4861 attttctcca aatactttca attattgtga aatatttccc tttaattaat caaatttttt
     4921 ataaaagtaa tatataaaat aaattgcttg aagtattata tttagatatc ataatttatg
     4981 gaagaaaaat taattaaaaa ggatgagatt ataatggggt tactggttcc gagagctaat
     5041 acttatacta tacctttaac gaaacgccaa ttaattgcta aaagatatca aaggattact
     5101 agagccatca atagagagtt ttggaattct gaaagtgata ctgctcacag tttatacgta
     5161 ggttcttatg ggagaggaac ggcaattagt actagtgata tagatataat agttgaatta
     5221 ccgatggcag aatttgacag attcaaaaat tatttatcaa atggcccgtc taagttatta
     5281 caagttataa aaaacgcatt tcaggaaata ctaccaaata gtgatattag agctgatgga
     5341 caagttgtta aaataaattt ccatgatgga atcaaatttg aaatagttcc agcttttaat
     5401 gaaaaagatt attggggtga gtcaaaaggg tttatttatc cagattcaaa catgggaggg
     5461 aactggaaag ctacaaatcc caaaaaagag caagaagcca tgaaattaaa gaatacaaaa
     5521 agtaataatt tattatatgc tacgtgtaaa cattttcgac atgtacgaga tacagaattt
     5581 acgagttatc atctttcagg aatagtaatt gacagtttcg tatatgaggc tatgggaaat
     5641 tggaaattcg ttgaaaataa cagtgggggt caaaacatta gttctgtatc ttatgagaca
     5701 gctttattag aatactataa ctcacataaa gttatgggcg gtttaaattt atactctcct
     5761 ggaagcaatc aatttgttaa ttctgatagt agtatcatat gtttagaaaa agtattaaaa
     5821 aaaatagctt tataaagttt tatggaggaa caacatggga gacgaaaaaa agcgcttgga
     5881 agttcaatta cgtgaagcat atggacgagt aacgtatact tacaccactc atttaaagca
     5941 agccgataat attatttctg acaataaaat tttaaggtgt cttcaaattg gtttgtcagc
     6001 ccttactact ggaggggtac ttagtaccat tattactgat aatagtgagt taacctttgt
     6061 tgctggatta tgttcaacaa tttctcttgg attaaactta tactttaaga attttgatct
     6121 tgctgaagaa gctaaaaatc atcaaattgt tgccgataaa ttatggttga tcagagaaga
     6181 atacgtaagc ttactaacag atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag
     6241 agatcaatta atgaacgaaa ctcatgaagc atataaaaat gcccctaaaa caaatagtaa
     6301 aagctatgaa tcagctcaga aagcattaaa atctgaagaa gagcaatttt ttacagaaga
     6361 ggaattggat aagatgttac cagagcatct tagaaaaaag agttctaaat attgaactct
     6421 ttttttatgc ttaaaattct cttgtaattt ttctattcat ctatatttaa gaagaaatat
     6481 gatgatagaa acatctccta aagccaccgc aagccgttct aagcatagct tagcccgaca
     6541 ttgctagata tgctcatctc accatgaacg tttcctaaag ccaccgcaag ccgtcctagc
     6601 cattgcctag tccgacatcg ctagattagt gcatctcgcc ataaaagttt tctaaagcca
     6661 ccgcaagccg tcctagccat tgcctagtcc gacatcgcta gattagtgca tctcgccata
     6721 aaagttttct aaagccaccg caagccgttc taagcatagc ttagcccgac attgctagat
     6781 atgctcatct caccatgaac gtttcctaaa accaccgcaa accgtcctag ccatcgccta
     6841 gtgcctagtc cgattttgct tgatcggttc catttctgtt tatttagtgg gtcaacaccg
     6901 taatcttaaa tagccatcgc ctatttaaga ttactaggtc tgctttgaag gtaaaaagtc
     6961 tttcattctt caagactttt aggctttcaa gctacgcctg aaagcattga aattcatcgc
     7021 tctttatttg gttttgccat ttataattcc ttacgcccat tatctttttc gcctgtcttg
     7081 ccttgtcaga agtgtttaga gcttatctca tctttggatt aatacaagtg gcgtaaattt
     7141 ttttctaaac gcaagcgtta cgaaaataat ttacttaaac acttgtatta atccggcaga
     7201 taagaacgca ttctaatcac tcccgacaag gcaagacagg cgaaaaagat aaataaatga
     7261 acgaaaggaa gaaacataaa atgcaaaacc aaatagcgat gaagaaaaaa ccacctccct
     7321 ctaaaacaat ccaaacaaaa aagaattttg aaaaaccaaa aatcaaaaac agaaagaagc
     7381 gttcaacatg agagatagaa ttttagaagt taatcaagaa atggtaaaca agttaacaaa
     7441 tatcagtggg aactatgaaa agtacgatgg tactttactt gataattata tttttcacga
     7501 tacacaaaat atcagaattg gcagaagtaa accgagagcg ttcattctca tcaaagagaa
     7561 atatttgaat gaatggaata gcacctatga aatggttatg actgataact ttgaaaaggt
     7621 attggatttc agaaatgctt tgttgggttc tgatatggga aatagcagtc ttgaatctat
     7681 tgaagttgaa aacaagaccg agaatgaagg tgaaggattg attaagaatg tcctgaaaat
     7741 cgaacaaaaa tatatcagtg aagatagaag tttttataga gtcacaagct cggttggtct
     7801 agcaaacttt gctactaact taattggaaa caatgctagt gaatctttgc tagtgatgtg
     7861 tctgaataca aagaatgagg tcattgctta tagcgaagtt ttcaaaggtg gaattaatca
     7921 atcagtagcg ataccaagag agatttttca actagctttg ctaaacaatt cagcaaaaat
     7981 cgcagtatca cataatcatc cgagcggata tgaatttcca agtgaaaatg acaaaagttt
     8041 tactacgaat ttgaaaaacg caggaaaaac aattggtatt gaattgttag accatatcat
     8101 cgtaacaagt aaaaacacaa gctattactc atttcaagaa aatggttctt tataaaaatt
     8161 agagaaaaga ggaaaacaag atgggcgaaa gaacacaagc gttaattaca gtcaaatatg
     8221 gtaacagcaa aacgaaagta agcgtgtcaa ttcattatca gtggggatat ggtcgattca
     8281 tgttgatgga cgttttgaat caaggaatat atttaatcaa taaagcatat gacttacgag
     8341 atgaatcaag agaagaatta gcaaaaggaa tctttgatag aagtacagga gttatcaatt
     8401 ttgttaccta tgaatctgga gtgagtgctt atgatgaaac agggtttggt gggcgctatg
     8461 aaccttatca agaaacgatt gaagatgtga tgaagttttc agataacaat gatggctatg
     8521 ctcatttaga gatggtgttc gatgaaaatg gatttagtct agatgataaa accagattgc
     8581 gattgtttga ttttgagggt caacagatag gactaaacaa gttttgtgaa agcggaaaag
     8641 agtttgctac gaaagatttt attaagggat acagattatt gctagaaaat tatggaataa
     8701 acctagaaga agagagcgtg aaataagtgt ttgaaaaaag aaaagaccag ttccctaatt
     8761 tagaagcgaa aagtcaaaaa gtgattgatg gtgaaatgat tgaattattc caaaaaagtt
     8821 atggaaccat agaaactacc gcaagttgca aatactactt tcaaggaaag agaaacacgt
     8881 ttaaaggctt tgccgattct gatgaagaat tggcaaagaa aatcattgat tggcttgtca
     8941 gcagtgaaca cgtagcaaga gaagataccg ttagaacaac gattgagggt gaagtcctaa
     9001 caggtactag atgtgtttgt tgtaatcatg aatttgtaga agatgaaggt tgctaccgaa
     9061 tcaatgaata tggtgatttt atttgccaga gttgccacga gatagaattt ccaaccgaaa
     9121 gaagttggtt tgaatacatg ctacaagatt atgagatgga aacagaaaat ggtgaagtag
     9181 ttttttgtac caaagaggaa ttgagtaaga gaagcgatga tgaaattgaa gattggtgtg
     9241 tgtggttagt agggacagat gattgcgttg catattacac aacagcagaa taggagtgtg
     9301 aatcagcatg gaaggaatgg attttataga tcatgaagat ttgattgatt ttggttatac
     9361 atggaaagga atggtaggta ttagcagaag tcttgctaat gccttttacg aaagaaatta
     9421 tgcagtctat gtcctttatg atgacaatac agaaagcctt gttgatgaag agtataaatt
     9481 ggatttagag aatgttttat atgggataga aaaagaagat ttagcaaaat atattttttc
     9541 atggttgggt caataaacga aagagggtag caacatgaca aaattcaaaa gtaaaggtat
     9601 gcaatggtgt tcttattatt atgttgggaa tgatgaagag cttttgagaa aagccaaaca
     9661 aaaaggttat ccagataaga tgttaaaaca gattggctat tacatgccag aagagagaat
     9721 agaaaaagaa tcttgtaagg ttctttttga tagtgaacag ctattagttt tgttaactca
     9781 aaataaattg atttgtcaat ttgaaaataa ttaatatggg gtgaagaacc atgaatgaga
     9841 ataagatggt attaagtgaa tacgcaagat tgagaaccat taattggttg aatggtatgc
     9901 aaagtagtgg cgcttttgat ggatttgatg aggagaatat ggactttgaa agtttatcga
     9961 atgaggatat tagatattat tttatcgaat ggtattttac agaaccagat gaagaggtgg
    10021 aagatgaacc agaattaatt ttagaacgtc aaagaatttt attcagtgaa caagatagta
    10081 agaggttgta tggtttatat gacttacaac tgaaaggatt aaaagcgcag tatgttgctg
    10141 atagttacga tgatatggta gaacaggggg cagatagggt tgtcacttat tcagaagatg
    10201 atgaagagga atgggaaaac tattcagatg atgagatatg ctttaatgat gggtatatgc
    10261 ctattgaaat caccgaagaa gaatatgata aagtgtcgga tagcgatgag attggtatgt
    10321 gtacggttgt aagtggattg gatatagatg tccaaagagt agaggttgca taccagaaga
    10381 tgaaagaacg tataaaaagt aaagtagaat cttgatttga ttggattgtt tgcaaagtag
    10441 ctaattaaga gtataatata atcataaatg tagttaatgg atataataat gagtatatta
    10501 tactcaaaag aggtgaacga aatgaaggta aaaaatccaa aagaaaaaag cagaattcaa
    10561 gtcggaatag acaaggattt aaaagaaaac gcagaaatga tattggaaga gctagggtta
    10621 aatccaacaa cagcaatcac aatcctttat aagcaagtgg tcgctagagg tgagtttcca
    10681 gtagagataa agctaagcga agaggaaaag caaggaatca gattgcaaca gctaactatg
    10741 gatatgccag ttgatgttct agatacagat gaaaagttag aagagtggtt caatgaagcc
    10801 taatgagata gtaacggtct atgttgcatt tacagataaa aatggtggta aaagaagacc
    10861 tattttggtg gttagtgata aagaagatag agtagaattt tttggaatca caagtcaata
    10921 tgagaataaa tcagaccaaa taaagagagt gtattttcct attgcagaat gggaaaaagt
    10981 aggtctaaag aagcaatcat ggattgacgt agggtcttta aaggctattc ccaaaagccg
    11041 agagaacata tcatttaaaa aggttggtat gctatcagct attgatacga aaaagctaaa
    11101 taggtttatc gagagattac aaagaaaagt ctaacaaggc ttttcttttt ttgtttatat
    11161 gtatatta
//
LOCUS       DACCIQ010000078        11123 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCIQ010000078 DACCIQ010000000
VERSION     DACCIQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.080"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            266..535
                     /gene="rpsN"
                     /locus_tag="IVM62_002143"
     CDS             266..535
                     /gene="rpsN"
                     /locus_tag="IVM62_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6846096.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            550..729
                     /gene="rpmF"
                     /locus_tag="IVM62_002144"
     CDS             550..729
                     /gene="rpmF"
                     /locus_tag="IVM62_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6846097.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            761..1138
                     /locus_tag="IVM62_002145"
     CDS             761..1138
                     /locus_tag="IVM62_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846098.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1159..1308
                     /gene="rpmG"
                     /locus_tag="IVM62_002146"
     CDS             1159..1308
                     /gene="rpmG"
                     /locus_tag="IVM62_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6846099.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1332..2315
                     /locus_tag="IVM62_002147"
     CDS             1332..2315
                     /locus_tag="IVM62_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6846100.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2345..2467
                     /locus_tag="IVM62_002148"
     CDS             2345..2467
                     /locus_tag="IVM62_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6846101.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2485..4008
                     /locus_tag="IVM62_002149"
     CDS             2485..4008
                     /locus_tag="IVM62_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6846102.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4032..4271
                     /locus_tag="IVM62_002150"
     CDS             4032..4271
                     /locus_tag="IVM62_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846103.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4464..4925)
                     /locus_tag="IVM62_002151"
     CDS             complement(4464..4925)
                     /locus_tag="IVM62_002151"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6846104.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5120..5338)
                     /locus_tag="IVM62_002152"
     CDS             complement(5120..5338)
                     /locus_tag="IVM62_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846105.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5456..7072
                     /locus_tag="IVM62_002153"
     CDS             5456..7072
                     /locus_tag="IVM62_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6846106.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7313..8653)
                     /locus_tag="IVM62_002154"
                     /pseudo
     CDS             complement(7313..8653)
                     /locus_tag="IVM62_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8929..9084)
                     /locus_tag="IVM62_002155"
     CDS             complement(8929..9084)
                     /locus_tag="IVM62_002155"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6846107.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9380..9712)
                     /locus_tag="IVM62_002156"
     CDS             complement(9380..9712)
                     /locus_tag="IVM62_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846108.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10226..10417)
                     /locus_tag="IVM62_002157"
     CDS             complement(10226..10417)
                     /locus_tag="IVM62_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846109.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10511..10897)
                     /locus_tag="IVM62_002158"
     CDS             complement(10511..10897)
                     /locus_tag="IVM62_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6846110.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3689 a         1761 c         2149 g         3524 t
ORIGIN      
        1 gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag
       61 agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt
      121 ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca
      181 actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat
      241 caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa
      301 acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga
      361 tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga
      421 tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt
      481 tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga
      541 ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga
      601 actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga
      661 cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct
      721 tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc
      781 tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg
      841 cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca
      901 acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga
      961 tgatttttta agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat
     1021 cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc
     1081 tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt
     1141 attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa
     1201 cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag
     1261 tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa
     1321 aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa
     1381 caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa
     1441 ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac
     1501 gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa
     1561 tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca
     1621 ttgaaactac aggtatagca gatccccaac caatcattca aacactttta acagcgcctc
     1681 aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg
     1741 aacttattga aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg
     1801 tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa
     1861 gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg
     1921 atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa
     1981 atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc
     2041 tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga
     2101 ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg
     2161 attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac
     2221 aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac
     2281 agagttttga taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt
     2341 tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa
     2401 aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt
     2461 agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca
     2521 gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa
     2581 gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc
     2641 gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat
     2701 gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac
     2761 gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat
     2821 gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat
     2881 cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg
     2941 gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca
     3001 aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac
     3061 tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt
     3121 ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa
     3181 gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc
     3241 aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa
     3301 gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat
     3361 aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact
     3421 atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct
     3481 aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa
     3541 tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa
     3601 gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac tactggctat
     3661 aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt
     3721 gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga
     3781 aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat
     3841 attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt
     3901 ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca
     3961 gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt
     4021 ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt
     4081 atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg
     4141 ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt
     4201 gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct
     4261 tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc
     4321 tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata
     4381 aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt
     4441 tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag
     4501 tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag
     4561 ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct
     4621 ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg
     4681 ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt
     4741 ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc
     4801 caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta
     4861 aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg
     4921 tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca
     4981 ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc
     5041 aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt
     5101 gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca
     5161 taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta
     5221 aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt
     5281 cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa
     5341 ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact
     5401 catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa
     5461 aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa
     5521 tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga
     5581 agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact
     5641 tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact
     5701 agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag
     5761 caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag acattgattt
     5821 gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga
     5881 taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga
     5941 agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt
     6001 aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa
     6061 gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat
     6121 tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga
     6181 gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt
     6241 tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa
     6301 ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt
     6361 ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt
     6421 agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga
     6481 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
     6541 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
     6601 taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc
     6661 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
     6721 tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
     6781 aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt
     6841 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
     6901 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
     6961 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
     7021 gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa
     7081 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
     7141 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
     7201 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
     7261 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
     7321 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt
     7381 tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat
     7441 agccataggc aattcgtttt agtactttga tatggttatt caaacattct atagggccgt
     7501 tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga
     7561 acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
     7621 caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct
     7681 ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc
     7741 gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta
     7801 gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
     7861 cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg
     7921 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
     7981 ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc
     8041 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
     8101 tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca
     8161 ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc
     8221 gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt
     8281 cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac
     8341 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
     8401 taatccgcag aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
     8461 agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt
     8521 gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa
     8581 aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact
     8641 ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga
     8701 ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt
     8761 caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat
     8821 cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa
     8881 caccttcatc tctattttac ttttatattt gttggagtcg attaattctc atttgattga
     8941 tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg
     9001 tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct
     9061 atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat
     9121 ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat
     9181 ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca
     9241 aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac catttctttg
     9301 attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc
     9361 atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt
     9421 ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct
     9481 cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt
     9541 ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa
     9601 ttgttggacg agagatattg tattcttcag caattttctt ggtagttact tttttctcaa
     9661 ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc
     9721 attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc
     9781 agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct
     9841 cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg
     9901 tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta
     9961 ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta
    10021 ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa acgttgataa
    10081 ttaaaatcat tcgttttaga ttctatgaat cattatagaa cgctgtaaaa tcgatacttt
    10141 ataattataa agaagatagt ttcaaattta ttgttcgaaa tttgaaacta tctttttact
    10201 tatgtcacag actcccttag ttgagttatt ctttaatttc gaatttcgag atagctagat
    10261 atattgcaag catacattct tgataacaat cttgatcata ttttccagtt ctccaggatt
    10321 gcttatgaat caaagagcta aatcttttga gaattgcctc aacagcttct tcatttccag
    10381 cctttgctaa aatgaaatct tcttttaaag aattcataaa tctaccttct tcccatggac
    10441 ttataatcta tttctttggg aaggaggaga acgagcaaag aagcatacaa aattagttat
    10501 tatgcagata ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg
    10561 tcgcaccctg tcttgtaatc cccagaacct ttccaatttc tttatcagtt ttatcaaaga
    10621 taaatttttc gataagaaaa atttgttcag gttctttcaa caaaggaagg atttttgcta
    10681 attttatatc ttcaacaagg agagtcacat ttaaaacatt aatcggttgt ttaaatttta
    10741 tagtatcttt agtaaacaaa tatggtggga gttgatcaag ttgttcatgt ttttgaattt
    10801 cttcttgttt tttataataa gtattagcgg tgttcaaaat gaccttttca aagaatcgaa
    10861 tcaactgggc atttacgcgt tcgtcgttat ctgacatatt gcacacctcc tccattaaga
    10921 tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg
    10981 aataacttct ataagctcac atgcattgat aggactgctg atgtttattt tcaaaaatgt
    11041 ttttttagtt gataatggat agttatcttt atacttagaa aagaaaattc atatatcttc
    11101 ttaaatatca aacatgtcct tat
//
LOCUS       DACCIQ010000079        11064 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCIQ010000079 DACCIQ010000000
VERSION     DACCIQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.081"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            31..900
                     /locus_tag="IVM62_002159"
     CDS             31..900
                     /locus_tag="IVM62_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP6846111.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            920..1267
                     /locus_tag="IVM62_002160"
     CDS             920..1267
                     /locus_tag="IVM62_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846112.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1280..2542
                     /locus_tag="IVM62_002161"
     CDS             1280..2542
                     /locus_tag="IVM62_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846113.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2622..3998
                     /locus_tag="IVM62_002162"
     CDS             2622..3998
                     /locus_tag="IVM62_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP6846114.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            3995..4624
                     /locus_tag="IVM62_002163"
     CDS             3995..4624
                     /locus_tag="IVM62_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846115.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4643..5122
                     /locus_tag="IVM62_002164"
     CDS             4643..5122
                     /locus_tag="IVM62_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846116.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5624..5677
                     /locus_tag="IVM62_002165"
                     /pseudo
     CDS             5624..5677
                     /locus_tag="IVM62_002165"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5681..6280
                     /locus_tag="IVM62_002166"
     CDS             5681..6280
                     /locus_tag="IVM62_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6846117.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6296..6772
                     /locus_tag="IVM62_002167"
     CDS             6296..6772
                     /locus_tag="IVM62_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846118.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6789..7691
                     /locus_tag="IVM62_002168"
     CDS             6789..7691
                     /locus_tag="IVM62_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846119.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7764..7955
                     /locus_tag="IVM62_002169"
     CDS             7764..7955
                     /locus_tag="IVM62_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846120.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8119..8706
                     /locus_tag="IVM62_002170"
     CDS             8119..8706
                     /locus_tag="IVM62_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846121.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8706..9605
                     /locus_tag="IVM62_002171"
     CDS             8706..9605
                     /locus_tag="IVM62_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP6846122.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9605..10519
                     /locus_tag="IVM62_002172"
     CDS             9605..10519
                     /locus_tag="IVM62_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846123.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10571..10786
                     /locus_tag="IVM62_002173"
     CDS             10571..10786
                     /locus_tag="IVM62_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846124.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4247 a         1497 c         2253 g         3067 t
ORIGIN      
        1 ttatgataac acaataaaag gaatggtggt atgaaaaaga agaagtttag ccgatcgttg
       61 ttaggaattt tggttttagt tgcggttggt cttagtggca agggttactt tcagaataac
      121 agtactcagg aacatgattt tcagatcaaa agtagtgttc aggtaggtaa taaagcagac
      181 agtgaatcaa aagaattttt agggatacgt tatgatccaa acaaatatcc aaataatatc
      241 gtaaacatta atgatgggaa atatacactg acaaaagaag accatgaagc attatcagaa
      301 aaaggcagtc aaaccttttg ggtaaattat gcagatttag atcaacttgg aagagcagga
      361 gatgttgaag cgttgatcac ctatgatgct gtaaattctc actcatctaa aaatgtggaa
      421 cgtccatctt tcaaaagttc tgtccatatt gcaggagaat acaaggatgg agtatttgat
      481 caagagagag atacttgggt cggcaaagat tcaaacaatg aaatcactca gctagaaggc
      541 tataaaggtt atctctataa caaaagtcat tcattagcat ggtcgctagg aggagatatg
      601 gaggtacaca atttaacact tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat
      661 ggagggatgg cttactcaga aacgattgta cgtgatgcaa tctataaaaa taagaatgaa
      721 aaagtattgt ataaagttga tccaatttat aaagggaatg aattagttcc tcgtggttct
      781 catgtcgaag ctttctcagt taacgataat ggaaaaacgg taaaccttaa tgtttatgta
      841 tttaacaatc aaaaaggaat caacatcaac tatcaaaatg gtacttgggt aagagattag
      901 aaattgagaa ggtgaagaat tggaaacaat aagcatcaat ggattagata aatcagttgc
      961 aaatcgaata aaaaaaggaa atggctacta tcttctgaag aagggtaaat attcagttgc
     1021 tagagagtta gaaacaatga gtatcgtgta tgtagctaag aatcattttg acgtagaaca
     1081 ggctattttt gatccagatt ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt
     1141 tagtacaaaa ggggcgatga tcattgagag agaaatgtta gacgaaatca gagtaatcat
     1201 tcctaaagat acgcagatgt gtgaggacag cataagtgac gatgaaccaa aatcagaaag
     1261 aaaataagga gatattcaga tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag
     1321 cgatggagaa aaatattatc ctgtagccag tttttctaaa tcagtatatc tggttggacg
     1381 tgcattggta gttggtgcaa ttggcatata tattttgcat tgggtaacaa aggttgtaca
     1441 actgcttgta gataccttag taagtattct tgtctcgtta aatgatgtta acggaacaga
     1501 tgatttagga ttaaaagaga tgtttaaaga gttactcaat gcgttttttc ccaattattt
     1561 aagttattca tataatgtac aaagcttttt tgcaaagcta cacttggtag acatctctat
     1621 tttagtatta gaagcaggaa cggtattctt tgttggatat ttggttactt tgatgttcag
     1681 gcatcactat ggagaacaaa gaccattttt tgatgatatg gaaagtagaa ggttgaaaag
     1741 agaagctcta gaagctatgg atgtgaagta tgatccaaac aagattatag gagatgaaac
     1801 gaacaacaaa aaaagaaaac gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct
     1861 taaaaatatc aaaatttctg ttcatacaag acgtgatcta agtgtcaatg aagatattcg
     1921 agaatatatc gtaaaaatcc cgcaaccaaa agggaagaaa gttagagaaa cagttttacg
     1981 acaaattcaa gatttagggt cgatcctgac aagagcaaca agcggacgag ccaaatttgg
     2041 tgaaaaagaa gatgaagttt caggcgatta tttcattttc cgagcatcag taaatattga
     2101 aagagaacgt aaggaaagag taagtaccaa aaacaaagaa acggaaagag caacatcttc
     2161 aatcgcagtt tcaaattcac aattttcttt tccaatcaat ctctttgccg ataattccaa
     2221 aaatatccaa aaccaaaccg aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt
     2281 agaactttat ctatcttctg ctgacgtacc tgcaagtcta acagatatgc acgttgggaa
     2341 tacatctatt gaatatttgt ttactttatc tcgtaccaga aaaattcaac taagctcaat
     2401 gaaagaagaa attgaaactt acttgcatat atcaggtgta atcgtgcgga tcaacgcagg
     2461 gcgtattcaa attactttac cattaggaga aaatgatcgt attccaattg atgtaccatc
     2521 aatgatcaat agcgtgtttt agaagacatc acaaatagaa acagaaaata caaactacca
     2581 gttcacaaac agaaaagtac cgagaaaagt gaggaataga aatggctatg aaattattag
     2641 agaaagtcaa aaagagagcc gaaaaagctg aatctatttt gctagataaa gaacctaata
     2701 taaagcttaa tggaaaattc agtgtcaata tgttgaacac aaaggctaaa gacccaacag
     2761 atgctatttt tggaattaag cctgatggca caattgctca tttttgcatc agcgaaatgc
     2821 cacacatgtt ggttgcagga acgacaggat cagggaaatc agtattactg aatgaaattc
     2881 ttgttacagt aatgtgtcac tcaacgcctg atgaattaaa aataggaatt gtagacccaa
     2941 aaagggttga atttggaaga tacaaaaaat tgccgttcat gttggctgat ccaattacag
     3001 atatggatga agcttatgat ttttttgaat atcttgtgat cttgatgcat gaacgttaca
     3061 aactgatgga aaaagcaggg ttacaaaaca tcacgctata taacgaatat gccgaaaaaa
     3121 atggattaga tagatttccc tatgtgatct tattagttga tgaatattca cagttggttg
     3181 gaacacataa agaagtagaa ggtttgattg ttcaattagg acaaatggca agggcagcag
     3241 gtattcatgt tatcttagca acacaatctc ctcgatcaac agttgtcact ggtattatca
     3301 aagccaattt tccgtcacgt gtttgcctta tggtagcaag tgatttagaa agtaggattg
     3361 tattagatga aggcggtgga gaaagtctaa gtccaaaggg agatatgctt attaagcttg
     3421 ttgatggaag tatggttaga gcgcagggag cttacatcag taacaaagaa attgaaacaa
     3481 tcttcaatca ccttagaaat actatgccag aaccagaata tgttgattac cgagcaatcg
     3541 tagctgaaac tcgtggtgag ggcggtgaag acgaggattt tggtggaatt ggtaaagctc
     3601 ctgtaagaaa atctgctaaa agaaatttga acacaccttt agcaaatcca tcatctaaag
     3661 agccatcaat tgaaaagaag agtgcgtttg ctattaagct tgaaaagaac aaagaagaaa
     3721 aagtcgagca gggagctaaa acactagaaa aactgcaagc agaaaaacta gaaaaagttt
     3781 taagggcatt aggaaaagct gatcctgaac cgcaaacctc aaacaacacg aataataaac
     3841 agccgattgc atctaaaacc aaacctaaag ctaatgatcc aaaaggctca gaaaacgtag
     3901 tgatcacaac tgataaatct gaaaagaaac aaataaatgg aagtacttca cctatcatgc
     3961 aaaaaatgtt aaaagcaaaa aaacaggtgc ataaatgatc atcgtcagtt cttttttaag
     4021 atggcttact ccatataaat atacagatga agtagttgtg aaacgagcga agaaaagaga
     4081 taaaaaagcg gtactaactc ctaaacagat gcttgatcat ggcattttag gggtgaaggt
     4141 tttagaagtg aataaaccgt ttgaatcaaa tagtgcaaga gaaaagttgc ttggagctga
     4201 agccgatgtc gcatattttt accatgacaa aagcaagcat caaatagcta ttaaattcaa
     4261 tccaaaatcg gcatggcggt atcaagcaag agatgacttg aaacgtagga aacttagacc
     4321 aatgctctat ccagaaggaa aagaagcatt tgatagaacc cacatcgttc ccattggttt
     4381 tcatggtaca gaatcagata accgcctatt agtaggttgg aacagcaatc aaaatagagt
     4441 tcagatcaat aaatttgaga agaaagtaag gaaattcaac gatcgaaaaa cgatcctatg
     4501 gtttgcagat atttgtaaac agccggatgg aacagcaaag tgggaagcgg tgatatttga
     4561 cgaaaatggt aaaaaagtaa tgtctgaaac atggcatgat aaagctaaat ttgtttggca
     4621 gtagcagtag aggtgaaaaa gattgctaga actttttgaa gcaaatagaa caggctttaa
     4681 tcaaaagtta gaagtgggag aaactataga atataaagat aaaaactata ttattatcgg
     4741 aatctataac acgagaatta acagaatagg tgatccacgt ataaccagtg acttggtttg
     4801 tcaatctgtt aactattcac ctgatctgac aagcaggtat aagaaatacg tgttattaga
     4861 atatagacac aagattactg atgaaattgg tgtaaacaac acacgaaaca tatttaagat
     4921 cggaacagtg attccttata gtaacaacga agaaaaaata ttttatcaga tttacggaat
     4981 agaaaagttt gaatatgaac atgtcgatct gctagtgacg taccaaatgc gtttaataga
     5041 accttggtca caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa
     5101 cgtattagga aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta
     5161 gcaatgaacg taggtaagaa catgacaata aataataata accctaaaaa atataatgct
     5221 cttgtccaat gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc
     5281 aaatcaaaga gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag
     5341 ctgaggaaaa aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg
     5401 aatgcaattg accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa
     5461 aggttaattc gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg
     5521 gctaaaataa atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact
     5581 gaaattttaa aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa
     5641 tatggagaat cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa
     5701 tagtagtcga tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga
     5761 ttttttgcag attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc
     5821 tttaggtggt ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca
     5881 tattaacttg taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact
     5941 gaatgatcag tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg
     6001 aagcttagtt ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat
     6061 ttttggatgg attgctttat ttgcgattgc atacatccgt tttgatagca agaaacattt
     6121 attactaagt gtttttgtat tattgataat cggcatttta ttggtatcta cttttgaatc
     6181 aggcgaggca aatgtcttgt cacatgtaat cggatttgtc tgtggaatga tcttgggatt
     6241 tattctaaag aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc
     6301 aaaaagaaaa gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca
     6361 aatactgtta agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat
     6421 gtacgaacaa cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa
     6481 aatctatgaa gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg
     6541 gatacttgaa tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct
     6601 ttttgaattt ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga
     6661 agcgtttgat acgctaagta aggatagcaa atccatgcga gatttattga ttacttatct
     6721 aaatgagcat gagtttgaac aaacgaaaac caaaaagaaa aataaagaat agaaagttgt
     6781 gatgaatcat gctattaacc ctgttaggtt atttatgttt aattcttgct ttttttgggg
     6841 caatcgctat aattattggt caatttcaaa caaataatcc gattatgcgt aacataggca
     6901 ttggaattgt gattgtaacc tttattgtgt ttaatgccac taagattttt ggaagtaacg
     6961 ataaacaatt tggtatatcg ggagttaata atagttatgc tccagattct ttgggaaggg
     7021 tagtaataga tggaacattt gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga
     7081 aggaagatgg caagaaagca tcagacgacg ttctcgctac tattaagccg tctcaatcac
     7141 aatttgaagt gttttataca gttaaagaga atagccctgt acacaagctg acattggttt
     7201 ttaacgttga tggagaagaa tactataaag atgtaaaaat aaataaaaca tcaacgaaaa
     7261 agattatcga atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt
     7321 ctactgtcga agcggaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt
     7381 ttgaatctgt ttctggttct atggctgatc ggtttaacac tgaagtcttc aaagctatca
     7441 aaactgttaa attggatact gaaaagcaaa tgattaatgt taccttattc aattcagttg
     7501 aatctatgga tcaggataaa aaagttacgg ttgcaagaga actaggaaaa agagtaattg
     7561 gtttgtatcg tggtactgct aaaccagatt cagacggtgc agatttgcca gttcatgtag
     7621 tttatgaaga cggtaaagtt tttggagatt ctacagcaaa cgaagcatta acgattaaat
     7681 tggctaaata gccagaaagt tataataaac aaatggtgtc acaccaataa tatataggtg
     7741 tgacaccttg gagatgatag ctgatggtag taaataaaga aaaagttaga aagtatgtga
     7801 ctttaaaaat agcttctgtt aaatatttag aaaagcgagc taaagaagaa gaaagagcaa
     7861 gaggtaagaa agtaacagtt tcagagttgt tagaagaatt gatcgagaat gatcaaacaa
     7921 taagacgtgc atttactaat aggtctatca gataacaata taattaataa aaaagaaaga
     7981 gtgatttggt cttagactaa atcactcttt ttatataaag tttctttaca ctcattccga
     8041 ataatgttaa aattctttat atgatatata gttccttgtc agaggaagag ataaccagca
     8101 atattggagg gataataagt ggaaaacaat aatgatatta gacattttag cattggggaa
     8161 agagttacaa cagtaagtga attatcaaag gattatagag ataaagaggg aacgattatt
     8221 tctcttaata agaatcgaag aggcgtttgg caatatgttg ttgatttagg tgatggcgtt
     8281 acagttgagt gtaaagatag tcaactgatc agaaataatg gcagtcattc cattgaaaaa
     8341 gcaattcttg tatacccaaa agtatcaagt aagatcatga atgactggtt gcttggaaaa
     8401 tttggtgagt acaataaaaa agaaatcgca gaagtgatcg gcaaatctta tccaacagta
     8461 ttagggtatc taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta
     8521 agagagcttc aatcattaat cgagtatcgg aataaacaaa taacaaatcc tgagtacctt
     8581 agaaaagaag atggtctatt gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa
     8641 aaggtagctc ctttaagcct tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa
     8701 gactaatgtg gactgtaact ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa
     8761 gaaggaatgc tgctgatgaa actaatgacg tagcagagaa aaggatttca acctactatg
     8821 ataaaaagta tcttgattat ttgaaaaaaa taaagagctt tatcgaagat caagatttat
     8881 tagatgatga attttactcg attgtaaatg atcctatggg cgcaattatg gaagtggact
     8941 tttactacca gataccaaaa agctataaaa aagtctataa cattatgaag acaacagcgc
     9001 cagatttaga taatttagta aaaagtgcta tggatggtat ttttaacatc tcagctatac
     9061 gagatagcca tatcacaggc ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa
     9121 cagttgtgag gatcaaacga tattcagaat ttgagtggga tacttctgaa ggattgaacg
     9181 gagatatatg ggaagctaca tttgatttta aaccagtagc aacgccaaga ccaaagtcta
     9241 aatttagagg aaatgggatt atcacttact atccaaaaga gtacaacgac tatttggaaa
     9301 atatcaagaa tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa aaggtaatga
     9361 atgctcctat gggggttata gctcagatag attattttta ccaagtgaga aaagataaga
     9421 aaaagcttga ttcactgatg cgtacttcac agccggatat tgataattta gtcaaaggaa
     9481 cattggacgg aatttttaac aaggaaatag gcatcaaaga tagcagggtt gtagggttaa
     9541 ttgcttttaa atacaatact tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg
     9601 gctgatgaat aataatgaaa aaattcttca tgtacttgat agctttgaaa taattcaaga
     9661 agaactaaaa aaatatcggg atgttttgga acaaagatat gattttgtca atcagcaaaa
     9721 gagtaatcac atggatttca ttctcaatat gaatgacttg aagaagaaat tagttgaacg
     9781 aaaagaacaa gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa
     9841 tgcaatggaa ctgaatgaag agcgcagggt tttagatcag ctgttagaac aattattggt
     9901 gatgtttcaa aaaggacgaa tagatgagga tttaattgaa gaaggattga gaaaatatcc
     9961 ttcaaactca ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt
    10021 cataccgcca gaggattttg aatcggcaat ggaatatata aaatattata gccaagggat
    10081 cactgctttt agagagtttg atcctgaaga tgttattcat gacttgaaca acctaaaaga
    10141 atggtgtgaa agttatggag tcgatgattc cggattggac tacttgatca gtattatgga
    10201 aatcgaagaa gagatgccgg ataagcctga tccaacagat attttagaat tgatccatga
    10261 agctagaaat ccaattgcct atatttctag aggatatact gtattggaat actataagcc
    10321 ttatatatca gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg aataccgctc
    10381 agtgttgaat gcaacgaata gattagaaaa agctgtttca gagctagatg cttattatcg
    10441 tgaacattac ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata tcagtaggta
    10501 tataaaaaaa gctgagtagg cggagttagc ccataaaatg gcaaaaggta acctgaaagg
    10561 ggcattttga atgaaaaggg atgtttttga attgtacgta ttagaacgtc tagaagacgg
    10621 catgatggca caagagataa ttgatgaaat tgatgatatg atcagcagat ttggtcaatt
    10681 ggagttgaat gatggaaaca aaaattcggt agtgctaaaa caattttggt tgaacaattt
    10741 ttcgtataag gattttatca aagaaaaggc aaatacattg tactgactaa attcacgagg
    10801 agcagtgatg agagcttatt gctaagagca gttaggctct tttttgtttg tgtaaaatta
    10861 tttgttttat aatacacatt tatacacaaa aaaatattga cagatagtac acaaatgtgg
    10921 taggatatat ttgtaagcaa gttagattaa caagttaaag gaagagcagt tatgaaaaaa
    10981 gaagaactaa tagatatatt tcaaattgtt gaacgagcaa ataatatggg aattatgttt
    11041 tttgatcgta tttctttaaa gatg
//
LOCUS       DACCIQ010000080        10771 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCIQ010000080 DACCIQ010000000
VERSION     DACCIQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.082"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1133
                     /locus_tag="IVM62_002174"
     CDS             <1..1133
                     /locus_tag="IVM62_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6846125.1"
                     /translation="RSFSLPVEVNYWDGTTKQYGDTDTPPQIKINIHEEIPMKEITSN
                     ASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPKKEKHTKQKNKED
                     IHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHILDKLFIKEGETLL
                     DIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDKCRVLLMDYRELK
                     GETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQQGGATNAWINKY
                     IFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFHQVQDQVTKEMGD
                     SFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1363..2010
                     /locus_tag="IVM62_002175"
     CDS             1363..2010
                     /locus_tag="IVM62_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP6846126.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2094..3038
                     /locus_tag="IVM62_002176"
     CDS             2094..3038
                     /locus_tag="IVM62_002176"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP6846127.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3173..4624
                     /locus_tag="IVM62_002177"
     CDS             3173..4624
                     /locus_tag="IVM62_002177"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP6846128.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4645..5481
                     /locus_tag="IVM62_002178"
     CDS             4645..5481
                     /locus_tag="IVM62_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6846129.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5481..6140
                     /locus_tag="IVM62_002179"
     CDS             5481..6140
                     /locus_tag="IVM62_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6846130.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            6266..6955
                     /locus_tag="IVM62_002180"
     CDS             6266..6955
                     /locus_tag="IVM62_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846131.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            6961..7917
                     /locus_tag="IVM62_002181"
     CDS             6961..7917
                     /locus_tag="IVM62_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP6846132.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            7961..8473
                     /locus_tag="IVM62_002182"
     CDS             7961..8473
                     /locus_tag="IVM62_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6846133.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8729..9277)
                     /locus_tag="IVM62_002183"
     CDS             complement(8729..9277)
                     /locus_tag="IVM62_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6846134.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9366..10547)
                     /locus_tag="IVM62_002184"
     CDS             complement(9366..10547)
                     /locus_tag="IVM62_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP6846135.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3614 a         1947 c         2136 g         3074 t
ORIGIN      
        1 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
       61 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
      121 agattacaag taacgcatct ttggcactag gagaagccta tatggatcat cgaatcgaga
      181 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
      241 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
      301 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
      361 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
      421 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
      481 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
      541 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
      601 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
      661 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
      721 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
      781 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
      841 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
      901 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
      961 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
     1021 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
     1081 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
     1141 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
     1201 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
     1261 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
     1321 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
     1381 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
     1441 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
     1501 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
     1561 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
     1621 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
     1681 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
     1741 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
     1801 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
     1861 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
     1921 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
     1981 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
     2041 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
     2101 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
     2161 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
     2221 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
     2281 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
     2341 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
     2401 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
     2461 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
     2521 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
     2581 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
     2641 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
     2701 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
     2761 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
     2821 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
     2881 tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa gggccggaaa
     2941 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
     3001 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
     3061 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
     3121 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
     3181 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
     3241 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
     3301 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
     3361 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
     3421 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
     3481 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
     3541 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
     3601 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
     3661 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
     3721 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
     3781 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
     3841 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
     3901 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
     3961 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
     4021 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
     4081 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
     4141 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
     4201 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
     4261 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
     4321 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
     4381 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
     4441 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
     4501 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
     4561 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
     4621 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
     4681 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
     4741 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
     4801 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
     4861 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
     4921 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
     4981 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
     5041 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
     5101 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
     5161 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
     5221 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
     5281 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
     5341 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
     5401 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
     5461 gctgagtcgg tgagtgagtg atgtactttc ctctaattga tttttccctc atgtggactt
     5521 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
     5581 ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
     5641 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
     5701 tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
     5761 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
     5821 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
     5881 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
     5941 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
     6001 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
     6061 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
     6121 ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
     6181 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
     6241 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
     6301 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
     6361 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
     6421 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
     6481 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
     6541 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
     6601 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
     6661 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
     6721 aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat tgaaaagacg
     6781 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
     6841 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
     6901 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
     6961 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
     7021 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
     7081 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
     7141 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
     7201 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
     7261 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
     7321 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
     7381 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
     7441 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
     7501 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
     7561 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
     7621 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
     7681 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
     7741 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
     7801 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
     7861 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
     7921 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
     7981 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
     8041 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
     8101 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
     8161 attagtctca agcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
     8221 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
     8281 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
     8341 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
     8401 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
     8461 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
     8521 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
     8581 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
     8641 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gacctctcgg
     8701 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
     8761 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
     8821 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
     8881 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
     8941 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
     9001 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
     9061 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
     9121 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
     9181 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
     9241 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
     9301 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
     9361 gtatattata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
     9421 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
     9481 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
     9541 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
     9601 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
     9661 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
     9721 gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg
     9781 ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac
     9841 atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct
     9901 aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg
     9961 gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa
    10021 actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat
    10081 tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta
    10141 taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata
    10201 aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt
    10261 ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca
    10321 aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa
    10381 acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg
    10441 aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca
    10501 agccctaatg tgacaaataa accaataata cctaataaat aactcaaata atcactccta
    10561 ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt
    10621 ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa
    10681 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
    10741 aagctaatag tttcgttttc aacatttata a
//
LOCUS       DACCIQ010000081        10753 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCIQ010000081 DACCIQ010000000
VERSION     DACCIQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.083"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..1591)
                     /locus_tag="IVM62_002185"
     CDS             complement(<1..1591)
                     /locus_tag="IVM62_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6846136.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
     gene            complement(1733..1957)
                     /locus_tag="IVM62_002186"
     CDS             complement(1733..1957)
                     /locus_tag="IVM62_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP6846137.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2208..3794
                     /locus_tag="IVM62_002187"
     CDS             2208..3794
                     /locus_tag="IVM62_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6846138.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(3860..5635)
                     /locus_tag="IVM62_002188"
     CDS             complement(3860..5635)
                     /locus_tag="IVM62_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6846139.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5848..6750)
                     /locus_tag="IVM62_002189"
     CDS             complement(5848..6750)
                     /locus_tag="IVM62_002189"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6846140.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            7029..9374
                     /locus_tag="IVM62_002190"
     CDS             7029..9374
                     /locus_tag="IVM62_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6846141.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(9711..10712)
                     /gene="ccpA"
                     /locus_tag="IVM62_002191"
     CDS             complement(9711..10712)
                     /gene="ccpA"
                     /locus_tag="IVM62_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP6846142.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         3265 a         2143 c         1984 g         3361 t
ORIGIN      
        1 ggtgagctgc aatgatagtt gttttctctt ttcttatttc ttttaaatta tgcaagatag
       61 cttcttctgt tttggcatct actgcagaaa gtgcatcatc taacattaat aattcaggat
      121 ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg tccgccagat aaagagactc
      181 cacgttctcc aactaaggta tcataacctt ttggtaattc cttgatttca ttgttgataa
      241 atgctaaaga agccgcatgc tttacttctt cttctgaaag ttttggatta gaaaaacgga
      301 tattatctcg aatggtcatc gaaaacaaga agtgatcctg aggcacatag ccgattgatc
      361 ctaataaagc attcaatgaa tattctttga tcgaacgtcc gccaaattgg attttccctt
      421 gataatcatc gtattcacgc atcagtaatt tgatgatcgt cgtttttccc gcaccagttt
      481 tcccaacaat acctaatgtt tctccttctt ggattgcaaa cttgatttgt tctaatgctg
      541 gtgattcttc tttcagataa ttaaaagagg aaatagctac attcagctcg ccttttgccg
      601 gggtctgcac tgcacccagt tgttccacga tatgcgtttt ttcatgcaaa agttcattga
      661 cgcgatcata agaagcattt cctctttcca atacgttgaa caatcgacca atcgcaaaca
      721 tcggccaaac aagcataccg atataactaa taaatgagac catctgcccg atcgagatcg
      781 tgccttccaa gacatacttt ccacctaaaa taattgttgc aacataagac agaccgataa
      841 tcaaggtaat gaatggatca aataaggagt ctaaaaaatt gactcgtttg tttttaataa
      901 ttgcatcttc aatcttttct tcaaaatccg ctagatcttc tttttcttgt ccaaatgttt
      961 taatgacttt gatcccagtg atactttctt gtgtcttatc attgatacta gaaaaagctt
     1021 cttgcgaatc acgaaaagca tcatggagtt tcgatcccag cactcgtgag gtgatggcca
     1081 gcaaaggcag aggaatcatc gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg
     1141 caatgatcgt tgttccgccc gtaatcactg aatctgcaaa ggtcaagata ccagctcctg
     1201 ctacattctg gatcgcattc aaatcattcg ttgcatgagc catcaaatcc ccagtccgat
     1261 atttttggta aaagagattg tccatttctg taaaatgttt gaataactgc tgccgaagtt
     1321 ctttctccaa ccgagctgca cttccccaaa tattcgtccg ccagatataa cgaaaaagat
     1381 attgtaaaaa agcagcgata atcaatagga agatccaccc gaaaatgatc tttattgata
     1441 aatttttatc ggcaatctca tcaatgatga tcccaatcac ttttggagga actaactgta
     1501 ccaaggccac tgcgaatagc gagaaaactc caattaaata atgtttcttt tcctgacgaa
     1561 aaaaccagcc taattttcta aaaatcgaca ttttactcct cctttgtctt tatcaagagc
     1621 ctaggattgt ctgtgcttgt cttttttgct gaataatctg aatagtctaa caaattacga
     1681 aataaaaaac gggctgaaaa aatcagcccg taaaaagtcg ttgggtcaga acctattttt
     1741 tcccttggtt cttcatttgc tgcatcatct ggcgaatttt cttctcagat ggtttttgcc
     1801 ccattgacat catcattgat cgcaacatgt cctcattaac gggaggattt ttttctaaat
     1861 agtctttcat atatttacgc gctaggaaaa agccacctac tgcgccgatt agcaaagcaa
     1921 taatagcaat aagtacgaca atacctgttg ataacatata aatctctcct ttctatttcc
     1981 gaattgctct caatgactta ttttactaaa gtttattgaa aaaagaaagg cgtattcttt
     2041 agtttcatgc aaaaaatagc ggttttttta gaggagaaca aaatttcttt cgtcatctag
     2101 caagacacgc cacgaaaata aattaggcat accgaatctt tttctttaat tttttctttt
     2161 tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat
     2221 ccaataaaca atttcataag catcgactta ttgaacatac aaatggttta tctttagctg
     2281 aagtaaatgg aacagttgat gtaccaaagg ggaaaagttt ttttaaaaca cttcttgctt
     2341 actctggacc tggtgcgtta gtagcagttg ggtatatgga tccgggtaac tggtctactt
     2401 cgatcaccgg aggacaaaac ttccaatatt tattgatgtc agttattttg atgtctagtc
     2461 ttattgctat gcttttacag tatatggcag caaaattagg aatcgtgagc caaatggatt
     2521 tggcacaggc aatccgagca cgcacaagta agacattagg tgtcatttta tggatcttga
     2581 ctgaattagc cattatggca acagatattg cagaagtaat tggtgcagca atcgccctct
     2641 acttactttt tgatattcca ttggtgattg ccgtctttat tactgtatta gatgttttcg
     2701 ttctcttact actgacaaaa gtcgggtttc gtaagatcga agcactggtt gtctgcttaa
     2761 ttttcgtcat tttattcgtt tttgtctatc aagttggctt gtctaaccca gattggcaca
     2821 gtttatttgc tggctttatc cctaacagcc atacattctc agactcacct gtcattggtg
     2881 gcgaaactcc gctaactgga gctttaggga ttattggtgc aacagtcatg ccgcataact
     2941 tatatcttca ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac gaagaagatg
     3001 tagcacaagc tgtacgtttt tctacttggg attctaatat ccagttgtca atggcttttc
     3061 tagtcaatgc actgctattg atcatgggtg tcgctgtctt caaaaccggt tctgtccaag
     3121 atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta
     3181 ttttgattgc tgtagcaaaa tctggtatct tgtccacttt atttgctgta gcacttcttg
     3241 cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggat
     3301 tcatccatat gcgtatcccg atttggctaa gaagactggt cacacgattg ctttctgtga
     3361 ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg
     3421 ctttgaataa cttaatgaat gaatcccagg tgttcttggc ttttgcgttg ccattttcga
     3481 tgattccttt actgatgatg accaacagcc aagcggagat gggtagccga tttaaaaatt
     3541 cattcatcgt aaaacttctt ggctggttct ctgtgattag tctaacttac ttgaacttac
     3601 gaggattacc aggacagatc gaaagtttct tcggcgagca agccacttcc gctcaactgg
     3661 cattggctga tcacatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga
     3721 cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcttttgcag gaaacggtag
     3781 aagaaatcga ataaaaactg atttttccac acaaagaccc ttgatagtta ggttaaaaat
     3841 ctagctacca agggtctttt tatttagaag ataaagcttt tttcagattt aatttactga
     3901 aaactagcat gcttgttact acgatgaatt gcgttaatgg cacgcctgca ccagtgtaga
     3961 cacttaaaaa atagtccttt ttttctggcg cataagaaca aaaccatgag acaccgaaaa
     4021 gaaaaaccaa ttgtgtcagt attaatccgg tgaccacaat caaagaggaa ctataatagg
     4081 cacgatcagc ttttgcaaat acggttccta gtatcataag taatatccct gctattgaag
     4141 caatcagcac gggaatagcc agaacttggt aaatttttat gatgacattc gcgacttttg
     4201 ctgattttgt taattggaag ttaggtgttc tcggttcatt actatcgatc caattctcca
     4261 ttagcggaag gtttaataat tcttgagcct gtttgacttg ttcttgcgtt ccgaaactat
     4321 aatttcctcc ttgatggaaa tctttatatc gccatgcata agcgtatcct gattgaagga
     4381 aatcgaatac gattggcaga tcactcatcg tctttccatc tcctgatccc atcagataga
     4441 actctttttt ctttttgatg gttcccttgt gataggcttc ttctaattct tggttgactt
     4501 ttttccaaaa atcattggtt tcttttgcat tgttttggta ataaccagat tcgcttacta
     4561 catctcgcaa tgcccaaaag atgatatctc cgggtaattc ttttactttt ccaccttgag
     4621 tccacgcgtg agtcgtatat aattcgtcaa tacgggtaga aacagtatgg aatgtaggag
     4681 atgcggctaa tgctttattt agtgcagcat gtgagaccca tatctcactg ttttctttat
     4741 tcaagttcgt ttcatcatct attcggatca gttgtttcat caaacgtcca aattctccac
     4801 tcgaacggtc attgatggtt gcaataccgt atgttgtctc atttttatat tcgatccact
     4861 gatagacgat ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc agccattccc
     4921 gtttcgagag aagaaagagt cgttttttta acttctttac ttctttgaaa gaaaagtctt
     4981 gccctagcaa gatctggacg aaactgatac ccaaagctac tacagtaaaa ggaagaagcc
     5041 agatagaatc ttcgcgaatg aaccaaaaaa aaggaaaagc gacagaaagc cctaaaaacc
     5101 atggcagcaa ttttctgcta gaaccgtatt tgcggtaata aatagctaat aaacaagcga
     5161 taatgatctc aacagccgga atcacgaggg cattacgata cgttcgagtg gaatattcat
     5221 gtgtaaaact aatcggagaa aacaaaaaat aaaaatagat acttaaaaga agatacttgt
     5281 tcttcacgat cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata ctgaacatgc
     5341 cgaataaaac tgcataagaa aaatgcagtt tatgtgcaag tactaaaaat aagctgtagg
     5401 agattccttt ggaaagtgtt gtagtcgtat actcccctag ccatttaccg tccaaaatat
     5461 tttgagcata gcgaatcagc aactgatcat cataggttgc cgacatgtct gttccataga
     5521 ttgctttact atataaatac aaacgaataa ttgtaaataa tagggctatg ccaagaataa
     5581 agtatgtatg tttcttagtc agttggttga tcaagggttt taaagtgttt agcatctttt
     5641 cgtccttcca atcacgtttt gccacttgct tgtgaaactt ttcgtttttt tacttttcta
     5701 ccagtatacc ctgtttgtct aaatttaaaa agagggggga gaagatgcat gatgaaaaag
     5761 actgcaaaaa gtactccttt gtttcttaaa caaaaaagga caaggcaatg tgcctggtcc
     5821 ttttttgctg ctgtcaaata gaaaatctta gagtatcact tcttctgcta ataactcagg
     5881 aaacacttct ggatcgaatg tttgcgcttt gaacatggcg atttcttttc catatggtgc
     5941 gtaagctttc tttttatctt tgccgacata gggagtttcc aagattttcg aaatattgtc
     6001 aaacattgga tcatggacga tcttattcaa cgtgtcgaat ccgattgttc caaaaccaat
     6061 attggcgtgc cggtctttat gcgaaccttg cggattttta gaatcattga tatggagtac
     6121 tttaagccga tctaacccga tgatcttatc aaactcttcc attacacctg aaaagtcttc
     6181 tttaatatta taaccggcat cattcgtatg acaggtgtca aaagtaacgg ataatttttc
     6241 attcaatttt actccatcga tgatttgcgc taattcctca aatgttcgac cgatttccgt
     6301 acctttacct gccatcgtct ccaaagcgat ttgtgccgtc tgttcttttg tcaaaacttc
     6361 attcaagcct tcaataattc tagcaattcc ggctgctgtt cctgccccta catgagcacc
     6421 tgggtgaagt gtaatttgag tagcgcctaa agcttccgca cgaacaatct cttctctcag
     6481 aaattggacg gcaaaaggaa acatttctgg tttgatcgta ttccccaaat taatgatata
     6541 aggggcatga acgacgatat ttgttagacc gttttctgcc atgtacgctt ttccggcatc
     6601 aatattcatc tcttcgatgg cttttcttct agtgttttga ggggcgcctg tatagatcat
     6661 aaaagtcgaa gatccataac tggctgcttc ttctgctgct cctaacagca ttttctttcc
     6721 gctcatgctg acgtgtgagc ctaataacat ggatggattc ctccttgaaa ggcaattgtt
     6781 actaacaatt cctatatatt ggtaagatga tatacaaaga aaatgattga tttttctttg
     6841 ccctgttctc catagtaact gaaattttct ctaaagaaaa gcttaagaac tcttttataa
     6901 aaaagatcat tttctcacgt tttaactcct ttaaaaaaga aattcctgcc aaaaacagcc
     6961 ttgtctgttt ttttgttcta ttatttgcta taattagcga aataaaacca ttagttgagg
     7021 tgaccctttt gccacgcaaa aatactcgaa aaaaacagaa tagaagaaaa aaagaaaaat
     7081 ggtttgttcc taagattatt tttagagttt ttcaatcatt gactgttttt attactgttc
     7141 ttcttttgat gttcgcagct ctaggtattg gaataggcgc tggctatttt gcctatctcg
     7201 tcgaagacac acaattgccg actaaaaaag cactgcagac agaactagga aacattacag
     7261 aaacgtcaaa gattgtttat gcagataata cagagatatc aaaaatccag acagatctga
     7321 tgcgcacgac tatttcatct gataaaattt cacctttatt gaaaactgca attatcagca
     7381 cagaggatga atacttcgat aaacaccaag gttacgttcc taaagctgtt ttacgagcat
     7441 tagtatctga agctactggt attggctctt ctggtggatc aactttgaca cagcaattag
     7501 tcaaacagca gatccttaca gatgaaacaa cattcaaacg taaagcaaac gagatcattc
     7561 tttctgcaca agtagaaaaa tacttttcaa aagatgagat cattgctacg tatttgaatg
     7621 tctcaccatt tggtcgtaat aataagggac aaaacattgc tggagtccag gaagctgcaa
     7681 gaggaatctt tggtgtagat gcaaaagatg ttactctgcc gcaagctgct tatatcgctg
     7741 gattacccca aagcccgatc acgtatagtc catacacgaa cacaggtgcg ctaaaagaag
     7801 atctttctgc cggccttgcc agaaaagatt ttgttttatt cagtatgtat cgtgaaggcc
     7861 aaattacgaa agaacaatac gaagaagcaa aagcctatga ccttacaaaa gactttctac
     7921 cacaacaaat cgcagaacag actgacagag aattcttgta ttatactgtg atgaatgaag
     7981 caacaagaat cattgcacaa caattggctg aaaaagacaa cgctgatatg tctgattcat
     8041 cgatcagtga cgcttattat caaaaggcac agcaaacgat ccaaaataaa ggatatacaa
     8101 tccattcgac aatcgataaa gatgtctatg ctgcattgca aaatggcgtt gaaaattatg
     8161 gatacatgtt agatgatggt gctggcagtc aagtagaaac cggaaatgta ttgatggata
     8221 acagaactgg ccgcatctac ggattcgttg gcggacggaa ttacagtcag aatcaaaata
     8281 accatgcctt cgatacagaa cgtcaagctg gctcatcaat caaaccagtt cttgtttacg
     8341 gacctgcaat cgacatgggg cttatcggat cagaatcccg tgtatcagat tatgcaacaa
     8401 cttggcaaga aggagaaaat gctggcgaga aaatcgtcaa tgctactaac gaaggatcga
     8461 acaccttcca aaccgtccgt gagtcactag aatggtccaa caatatcccg gcttatcact
     8521 tatatcaaga tgtgttgaat tccggtggat cgaaacaata tgcttacgag cactacttgt
     8581 caaagatgaa ctacccggca aatgacaatt ggggcgttga atctgctcct ttaggtacag
     8641 tagatgtcac gacgcttcaa cagaccaacg gatttcaggc tttagcaaac ggcggcgtct
     8701 atgaagaagg ctatatcatc gattccatca cagataacgc aggaaatgta atttataaac
     8761 acgaaagcaa ctctgtccgg atctattcgg aagcaacggc atcgatcatg aatgacatga
     8821 tgcgcagtgt gatcaatgca aaaatcacta caccattcaa agatgccatc tcaagcttaa
     8881 acggaaatct aggaaaagca gattgggtcg gaaaaactgg ttctaccaat gaataccgag
     8941 attcatggct aattgtctct actccgtcta ttaccatcag tagctgggca gggcacgatg
     9001 acaatactgg aatggacagc aaagcaagga tacgttcggc taattatctt gccaatctga
     9061 tcaatcaagc ttaccaagca aaacctgaca tttttggcac tgatgagaaa ttcgaattat
     9121 catctgatgt gatgaagaaa aaagtatctg gatttactgg tcaaacacct ggaaaagtta
     9181 ctgtcaataa aaaatccatc caaacaccag gaaaaacagt tgaatcgcta tgggcgaaaa
     9241 atggaccgaa gaaaagcgcc tttaagtttg gcgttggcgg taccgatgaa aactatacag
     9301 attactggaa caccgcttca gcttatgcaa gagcgaatca agcaaccaaa gaatcagatg
     9361 agaaagatga ttgatttaat agaatacaca gtaataaggg aatgtgacaa tagtattgtc
     9421 acattccctt atttttttga tgatggtgaa caaaatacag catctataca cggattgttt
     9481 tatatacgat aaatataatt tcaaccactt tctctctttt ttaagacaca aaaaaaggat
     9541 tataacaaaa ttttgtcata accccttttc ccaaataaac ggtgttctcc tgccaactcc
     9601 gaatcacgag tcacaatatt catcaaacat gagatgctgt tttttgaaat tgctccttgt
     9661 aacgcagagt taaacggcag gcacacaacc tctgaatggt tgtcctttta ttattttgta
     9721 gatccttttt cttcgattcc ataaggcaag ataatcgttt tttcttcgac ttcttcttta
     9781 ttcatcattt tcgtcaatag gcgcatagat accgccccta aatcgtataa tggttgtgtg
     9841 atacttgtta aacgaggacg agctacttct gtcagtagtg aattgttact tgtgatgatt
     9901 tcgaattctt ccggtacttt cacgccacgg tctaataaac catccaaaat accgatggct
     9961 aattcatcat ctgaaacata gacagctgtt gcaccgctat tatggatccg atcgactaga
    10021 gatagaccat ctttgaagtt gtattgtgct tcaaagatca atccttcgct atatggtagg
    10081 ttattttcgc ttaatgcttc tttatagcct ttcagacgat tttgtccatt gatgggatcg
    10141 atcaacgcac cactgacaaa agcgattttt ttattgccgc tttttgccaa tttgtttact
    10201 gcatcttttg ttgcggatac ataatcaatg tttacgctac ctacttgttc atctggatca
    10261 atagagccag ctaatacaac aggagttttt gaacgtgaaa attctccacg gatttcgtct
    10321 gtgatatggt gacccatgaa gatcacgccg tccacttgct tagccagtaa gttattcaat
    10381 acattgactt ctttttggtc gttgccatct gaatttgcta agataatatt gtatttgtac
    10441 attgttgcca catcatcgat accacgtgct aaagaagcaa agaacatatt gctgacatca
    10501 gggatgatta caccaactgt tgttgttttc ttacttgcca agccacgcgc aactgcgttt
    10561 ggacgataat ccaagcgatc gataacttcc aagacttttt ttcttgttgc tggtttgacg
    10621 tttggattgc catttacgac acgtgaaaca gtagccatcg aaacatttgc ctctctcgct
    10681 acatcataga tagtgattgt ttgcttttcc atttgtgttc tcctattcat attttatatg
    10741 ttttctgaaa ata
//
LOCUS       DACCIQ010000082        10661 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCIQ010000082 DACCIQ010000000
VERSION     DACCIQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.084"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            333..2279
                     /locus_tag="IVM62_002192"
     CDS             333..2279
                     /locus_tag="IVM62_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6846143.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IVM62_002193"
     CDS             2282..2737
                     /locus_tag="IVM62_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6846144.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IVM62_002194"
     CDS             2751..4079
                     /locus_tag="IVM62_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6846145.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IVM62_002195"
     CDS             4113..4397
                     /locus_tag="IVM62_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6846146.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IVM62_002196"
     CDS             4399..4914
                     /locus_tag="IVM62_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP6846147.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IVM62_002197"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IVM62_002197"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6846148.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IVM62_002198"
     CDS             5599..6171
                     /locus_tag="IVM62_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846149.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="IVM62_002199"
     CDS             complement(6358..7878)
                     /locus_tag="IVM62_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6846150.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="IVM62_002200"
     CDS             complement(8121..8306)
                     /locus_tag="IVM62_002200"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP6846151.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="IVM62_002201"
     CDS             complement(8290..8472)
                     /locus_tag="IVM62_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846152.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="IVM62_002202"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="IVM62_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="IVM62_002203"
     CDS             9426..10025
                     /locus_tag="IVM62_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6846153.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="IVM62_002204"
     CDS             10089..>10661
                     /locus_tag="IVM62_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846154.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DACCIQ010000083        10646 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCIQ010000083 DACCIQ010000000
VERSION     DACCIQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.085"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            34..657
                     /locus_tag="IVM62_002205"
     CDS             34..657
                     /locus_tag="IVM62_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846155.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="IVM62_002206"
     CDS             1106..2155
                     /locus_tag="IVM62_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6846156.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="IVM62_002207"
     CDS             2152..3033
                     /locus_tag="IVM62_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6846157.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="IVM62_002208"
     CDS             3036..3806
                     /locus_tag="IVM62_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6846158.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="IVM62_002209"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="IVM62_002209"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP6846159.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="IVM62_002210"
     CDS             5117..6280
                     /locus_tag="IVM62_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6846160.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="IVM62_002211"
     CDS             6397..6807
                     /locus_tag="IVM62_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6846161.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="IVM62_002212"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="IVM62_002212"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6846162.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9347..9664
                     /locus_tag="IVM62_002213"
     CDS             9347..9664
                     /locus_tag="IVM62_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846163.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9824..10060
                     /locus_tag="IVM62_002214"
     CDS             9824..10060
                     /locus_tag="IVM62_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6846164.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="IVM62_002215"
     CDS             10057..10455
                     /locus_tag="IVM62_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6846165.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3721 a         1772 c         2253 g         2900 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggct
//
LOCUS       DACCIQ010000084        10377 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCIQ010000084 DACCIQ010000000
VERSION     DACCIQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.086"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            77..817
                     /locus_tag="IVM62_002216"
     CDS             77..817
                     /locus_tag="IVM62_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846166.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1108..1776)
                     /locus_tag="IVM62_002217"
     CDS             complement(1108..1776)
                     /locus_tag="IVM62_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6846167.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            1910..2197
                     /locus_tag="IVM62_002218"
     CDS             1910..2197
                     /locus_tag="IVM62_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846168.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            2613..4151
                     /locus_tag="IVM62_002219"
     CDS             2613..4151
                     /locus_tag="IVM62_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP6846169.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            4148..5113
                     /locus_tag="IVM62_002220"
     CDS             4148..5113
                     /locus_tag="IVM62_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846170.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            5100..6422
                     /locus_tag="IVM62_002221"
     CDS             5100..6422
                     /locus_tag="IVM62_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6846171.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      6485..6589
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6663..7406
                     /locus_tag="IVM62_002222"
     CDS             6663..7406
                     /locus_tag="IVM62_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6846172.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            7421..8413
                     /locus_tag="IVM62_002223"
     CDS             7421..8413
                     /locus_tag="IVM62_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6846173.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            8425..9162
                     /locus_tag="IVM62_002224"
     CDS             8425..9162
                     /locus_tag="IVM62_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846174.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            9152..9874
                     /locus_tag="IVM62_002225"
     CDS             9152..9874
                     /locus_tag="IVM62_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6846175.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9958..>10377)
                     /locus_tag="IVM62_002226"
     CDS             complement(9958..>10377)
                     /locus_tag="IVM62_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6846176.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3442 a         1788 c         2171 g         2976 t
ORIGIN      
        1 tcagcgcatt gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt
       61 gagaaggatg gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga
      121 tgatgagtta ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga
      181 agatcttttg actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac
      241 gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc
      301 tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta
      361 ccaatcagaa atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt
      421 cagacaaagt gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac
      481 gaaggtgcgg gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga
      541 ccctagagaa agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga
      601 ctcttatctg ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc
      661 gtctgtacga acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa
      721 aaaatcgaaa aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca
      781 aggaaactct ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg
      841 ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta
      901 tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa
      961 taatggcaaa ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt
     1021 tttataaaga agattttact gaataatatc attttagttt acataatgat atttctgtaa
     1081 attaaataaa gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg
     1141 ttcaaatgtt tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag
     1201 atacttcact ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa
     1261 atgtgtgaga tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag
     1321 acttttgcca atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc
     1381 gcctgtgctt ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt
     1441 catcaaacta ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa
     1501 atgtaaaaag aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc
     1561 aaaaagaaac aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg
     1621 agcgaatcca aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag
     1681 cttagatgga atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat
     1741 tccgcacaat tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa
     1801 ttttcagcaa cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa
     1861 aaattatttt tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc
     1921 agacttattg acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt
     1981 ggctcaagaa caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt
     2041 tatcttttta tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt
     2101 gatgaagaac cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta
     2161 tggagtgaaa gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt
     2221 ttatgatgtc aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat
     2281 aatattcgtt gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc
     2341 tgtttctttt tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg
     2401 acaatttcgt atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat
     2461 ccgactttgt tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt
     2521 tttgttcgtg ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt
     2581 tatacttaaa gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg
     2641 aaagaaaagg cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt
     2701 tgctgattgc agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa
     2761 tggcagaagt cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat
     2821 taggtttact tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta
     2881 tttacgcttt gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt
     2941 ctattgaaga agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag
     3001 agcttcccat cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca
     3061 tcggtacggc tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt
     3121 taggtattga ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc
     3181 ttgcaattat attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca
     3241 tcattacttt agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct
     3301 tgtttaaaaa tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga
     3361 tccaaatgta taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga
     3421 attttggcaa gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc
     3481 ctgagttcac aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg
     3541 ctgatttgac aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg
     3601 atatgacatt attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat
     3661 cttttgcgaa cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga
     3721 tcaaagcggg attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag
     3781 aagcatatgg cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag
     3841 caatcaataa tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg
     3901 agtacgatct agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac
     3961 cgctgatgaa aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat
     4021 taagcggaaa aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa
     4081 agaaaaagcc gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg
     4141 aagatcagtg actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta
     4201 tgtcatcgat gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc
     4261 ttccggtagt ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc
     4321 aggagacatc tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg
     4381 gaatatggga tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa
     4441 tattgaagtc atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga
     4501 tgaacttctt caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga
     4561 gttgtctgga ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga
     4621 tgtgattttg atggacgagc catttagcgc attagatccg atttcaagaa actcattaca
     4681 agaattggtt ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa
     4741 tatggaagaa gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat
     4801 ccaatgtgac acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt
     4861 ctttgatgaa ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata
     4921 tttcttaaat gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa
     4981 agaagtatat tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag
     5041 tattgggtcg ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa
     5101 tgaaaacata tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg
     5161 gattgaaaga agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt
     5221 gtcctaaccg tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc
     5281 gggtaaaaca attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac
     5341 ttcaaaaatt caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag
     5401 cagaagctga aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg
     5461 aagtcaacga agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta
     5521 cgatcttacc agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc
     5581 ctgtactgcc taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta
     5641 cgattgccaa tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa
     5701 aagaatttga ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc
     5761 agtttgcttt tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag
     5821 tcggaaaaga gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga
     5881 atacagaatc tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag
     5941 tcaatgatta tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa
     6001 ggaaaacccc aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga
     6061 taacggatgc aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca
     6121 gtcctaaact tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg
     6181 tagagatgga tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg
     6241 ctgaattgac ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag
     6301 cagacgaatt gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt
     6361 tggatagcta tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat
     6421 aatcagcatg ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa
     6481 tgaatacctg tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc
     6541 attgaacctg tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt
     6601 actatgtcgt gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca
     6661 aaatgaagaa aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga
     6721 ttgttgtcat agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt
     6781 ctgcattagc ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag
     6841 cagtgcttgg tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt
     6901 attggaagtt tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa
     6961 tcatggtgtt agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga
     7021 tccttgttgt cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga
     7081 aagtatcaaa agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt
     7141 atcgaatata taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg
     7201 ctacttattg tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag
     7261 ggtattacat gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca
     7321 tttgtgtcat tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt
     7381 attatcgtat gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt
     7441 attaccgcta ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca
     7501 agaaataaag gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca
     7561 tactggcatc tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca
     7621 aatcatcgaa ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca
     7681 atttggtgta agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc
     7741 agtcaaagca attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga
     7801 cagtaacatc cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc
     7861 aagtgaagaa gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact
     7921 gacaatggta ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca
     7981 atggtacttt gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta
     8041 tatcccattg aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa
     8101 tgatcaactg atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa
     8161 aggctatcag gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc
     8221 aaaagaaaat gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac
     8281 ggatgatcct gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt
     8341 tatgcaggaa aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca
     8401 gtttataaaa taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt
     8461 gaaaacctga aagtattaga agacatccat ttcactatcc ctaaagatac ctttacatcg
     8521 attgtcgcgc caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag
     8581 cctatgactt ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca
     8641 tttagttata tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt
     8701 acgttatatc aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg
     8761 gatatctttg ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg
     8821 aagcagcgaa cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat
     8881 gaaccatttg gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag
     8941 ctgcctaaaa aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt
     9001 tatttatcag acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt
     9061 caagtgcctg aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa
     9121 acgatttatc agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga
     9181 tgggagtcag agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg
     9241 tgattcgcca gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt
     9301 aagttacggt atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt
     9361 tttgaaaaaa gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc
     9421 gatgactaca caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa
     9481 acctgttgta ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta
     9541 ccctaatcac tatttgatcc attggtattc ttctttggac tatcaacaag actatattga
     9601 tttaggctgt tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact
     9661 agctaaagca gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga
     9721 gtgggcttta ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt
     9781 aaagacaatc gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca
     9841 aaacttggaa cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc
     9901 gcacctcttt ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta
     9961 gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt
    10021 aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga
    10081 agaatctaat tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga
    10141 ttctttttgt gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc
    10201 tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac
    10261 cgtttcttgt acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg
    10321 ttcagcagct gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACCIQ010000085        10277 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCIQ010000085 DACCIQ010000000
VERSION     DACCIQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.087"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(368..1486)
                     /locus_tag="IVM62_002227"
     CDS             complement(368..1486)
                     /locus_tag="IVM62_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846177.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     NVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IVM62_002228"
     CDS             1957..3264
                     /locus_tag="IVM62_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6846178.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IVM62_002229"
     CDS             3383..4582
                     /locus_tag="IVM62_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6846179.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IVM62_002230"
     CDS             complement(4609..5877)
                     /locus_tag="IVM62_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP6846180.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="IVM62_002231"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="IVM62_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="IVM62_002232"
     CDS             complement(7094..7597)
                     /locus_tag="IVM62_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6846181.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="IVM62_002233"
     CDS             complement(7720..8484)
                     /locus_tag="IVM62_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP6846182.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="IVM62_002234"
     CDS             8592..8867
                     /locus_tag="IVM62_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP6846183.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="IVM62_002235"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="IVM62_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6846184.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         3089 a         2161 c         1869 g         3158 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacattaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataa
//
LOCUS       DACCIQ010000086        10237 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCIQ010000086 DACCIQ010000000
VERSION     DACCIQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.088"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            14..85
                     /locus_tag="IVM62_002236"
     tRNA            14..85
                     /locus_tag="IVM62_002236"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:gcc)
     gene            102..187
                     /locus_tag="IVM62_002237"
     tRNA            102..187
                     /locus_tag="IVM62_002237"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136..138,aa:Leu,seq:taa)
     gene            198..271
                     /locus_tag="IVM62_002238"
     tRNA            198..271
                     /locus_tag="IVM62_002238"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:232..234,aa:Arg,seq:acg)
     gene            281..354
                     /locus_tag="IVM62_002239"
     tRNA            281..354
                     /locus_tag="IVM62_002239"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:315..317,aa:Pro,seq:tgg)
     gene            374..446
                     /locus_tag="IVM62_002240"
     tRNA            374..446
                     /locus_tag="IVM62_002240"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Ala,seq:tgc)
     gene            474..547
                     /locus_tag="IVM62_002241"
     tRNA            474..547
                     /locus_tag="IVM62_002241"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:508..510,aa:Met,seq:cat)
     gene            561..634
                     /locus_tag="IVM62_002242"
     tRNA            561..634
                     /locus_tag="IVM62_002242"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:595..597,aa:Ile,seq:cat)
     gene            658..747
                     /locus_tag="IVM62_002243"
     tRNA            658..747
                     /locus_tag="IVM62_002243"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:694..696,aa:Ser,seq:tga)
     gene            760..833
                     /locus_tag="IVM62_002244"
     tRNA            760..833
                     /locus_tag="IVM62_002244"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:794..796,aa:Met,seq:cat)
     gene            852..927
                     /locus_tag="IVM62_002245"
     tRNA            852..927
                     /locus_tag="IVM62_002245"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:885..887,aa:Asp,seq:gtc)
     gene            929..999
                     /locus_tag="IVM62_002246"
     tRNA            929..999
                     /locus_tag="IVM62_002246"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:961..963,aa:Gly,seq:tcc)
     gene            1013..1086
                     /locus_tag="IVM62_002247"
     tRNA            1013..1086
                     /locus_tag="IVM62_002247"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1047..1049,aa:Ile,seq:gat)
     gene            1111..1198
                     /locus_tag="IVM62_002248"
     tRNA            1111..1198
                     /locus_tag="IVM62_002248"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1145..1147,aa:Ser,seq:gct)
     gene            1220..1291
                     /locus_tag="IVM62_002249"
     tRNA            1220..1291
                     /locus_tag="IVM62_002249"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1253..1255,aa:Glu,seq:ttc)
     gene            1314..1403
                     /locus_tag="IVM62_002250"
     tRNA            1314..1403
                     /locus_tag="IVM62_002250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1350..1352,aa:Ser,seq:tga)
     gene            1416..1489
                     /locus_tag="IVM62_002251"
     tRNA            1416..1489
                     /locus_tag="IVM62_002251"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1450..1452,aa:Met,seq:cat)
     gene            1508..1580
                     /locus_tag="IVM62_002252"
     tRNA            1508..1580
                     /locus_tag="IVM62_002252"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1541..1543,aa:Asp,seq:gtc)
     gene            1584..1656
                     /locus_tag="IVM62_002253"
     tRNA            1584..1656
                     /locus_tag="IVM62_002253"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1617..1619,aa:Phe,seq:gaa)
     gene            1661..1731
                     /locus_tag="IVM62_002254"
     tRNA            1661..1731
                     /locus_tag="IVM62_002254"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1693..1695,aa:Gly,seq:tcc)
     gene            1744..1817
                     /locus_tag="IVM62_002255"
     tRNA            1744..1817
                     /locus_tag="IVM62_002255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1778..1780,aa:Ile,seq:gat)
     gene            1829..1900
                     /locus_tag="IVM62_002256"
     tRNA            1829..1900
                     /locus_tag="IVM62_002256"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1862..1864,aa:Glu,seq:ttc)
     gene            2166..3359
                     /locus_tag="IVM62_002257"
     CDS             2166..3359
                     /locus_tag="IVM62_002257"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP6846185.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3489..4961
                     /locus_tag="IVM62_002258"
     CDS             3489..4961
                     /locus_tag="IVM62_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6846186.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5044..5832
                     /locus_tag="IVM62_002259"
     CDS             5044..5832
                     /locus_tag="IVM62_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP6846187.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5899..6567)
                     /locus_tag="IVM62_002260"
     CDS             complement(5899..6567)
                     /locus_tag="IVM62_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6846188.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6682..8394
                     /locus_tag="IVM62_002261"
     CDS             6682..8394
                     /locus_tag="IVM62_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846189.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8397..10169
                     /locus_tag="IVM62_002262"
     CDS             8397..10169
                     /locus_tag="IVM62_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846190.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2960 a         1852 c         2357 g         3068 t
ORIGIN      
        1 catctttaaa cacgcggaaa tagctcagtg gtagagcacc accttgccaa ggtgggggtc
       61 gcgggttcga accccgtttt ccgctttgcc aaagcaatcg cgccggggtg gcggaactgg
      121 cagacgcaca ggacttaaaa tcctgcggtg agtgatcacc gtaccggttc gattccggtc
      181 ctcggcattt caaatatgcg cccatagctc aactggatag agtgtttgac tacggatcaa
      241 aaggttaggg gttcgactcc tcttgggcgc gttaattaaa cgggaagtag ctcagcttgg
      301 tagagcactt ggtttgggac caaggggtcg caggttcgaa tcctgtcttc ccgataatta
      361 taaatttaaa cacggggcct tagctcagct gggagagcgc ctgctttgca cgcaggaggt
      421 cagcggttcg atcccgctag gctccatttc atttaaataa tgaatatctt tttcgcggtg
      481 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatcccctcc
      541 gccgcgattt ttaagagcct ggacctttag ctcagttggt tagagcagac ggctcataac
      601 cgtccggtcg taggttcgag tcctacaagg tccattgtgg cgttgtaagt ttcatatgga
      661 ggattaccca agtccggctg aagggaacgg tcttgaaaac cgtcaggcgg gtaaaaccgt
      721 gcaagggttc gaatccctta tcctcctttt tcatattatc gcgggatgga gcagtctggt
      781 agctcgtcgg gctcataacc cgaaggtcgt aggttcaaat cctgctcccg caattaaaaa
      841 ctaaaaacac tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg
      901 cgggttcgat tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct
      961 tccaagctgt tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat
     1021 agctcagctg gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta
     1081 ggcccattag tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc
     1141 ccctgctaag ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt
     1201 tcaatatttt gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc
     1261 ggtaacacgg gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat
     1321 tacccaagtc cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa
     1381 gggttcgaat cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct
     1441 cgtcgggctc ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa
     1501 aaacattggt tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg
     1561 ttcgattccc gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag
     1621 ctccatgtgt cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta
     1681 aaaccacagc cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt
     1741 tatgggccta tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc
     1801 gagtccattt aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc
     1861 tttcacggcg gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta
     1921 tctttttctt tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga
     1981 tgattgtgct aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca
     2041 aaaaaatgaa aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag
     2101 ttcccgaaag gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca
     2161 ttataatggt agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata
     2221 aaattgctga ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag
     2281 cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat
     2341 ctacgacggc atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt
     2401 atacacgtgc aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg
     2461 aacaatctcc tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata
     2521 aaaaagacgt attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg
     2581 tagatgaaac accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac
     2641 gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc
     2701 aagtaacggt tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt
     2761 caacacagca tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag
     2821 tagtgaagga agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc
     2881 cgactggccg atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa
     2941 tcattgttga tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag
     3001 atgcgacaaa agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg
     3061 ttgcagcagg gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc
     3121 aacctgtttc gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga
     3181 ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt
     3241 tgcgtcgtcc gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag
     3301 atttgccttg ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag
     3361 ttatattcag ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta
     3421 acctcttgaa aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga
     3481 ggaataagat gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa
     3541 cgttcatgac ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt
     3601 cattacatgg aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta
     3661 tgcttactcc gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt
     3721 ttgggacatt attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg
     3781 ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt
     3841 tgacgatcat cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata
     3901 gttcagcctg ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa
     3961 cgatcagctg gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt
     4021 tgatttggct atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt
     4081 ttggtagttt tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag
     4141 gagacgaagg aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag
     4201 gtatttttgt ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca
     4261 aaaatccact atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg
     4321 gaatcgatgt ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg
     4381 gtgggctggt attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca
     4441 agtggatgac tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta
     4501 ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta
     4561 tatcaacggt catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac
     4621 aaaatagtgt atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa
     4681 caattggtca aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac
     4741 gagaacttgc agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc
     4801 cacaaacggc taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg
     4861 atgggttaca ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa
     4921 cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac
     4981 gtagccgaat cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa
     5041 aatatggcat ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta
     5101 aatgtgatac taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca
     5161 acggatatac ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa
     5221 agacgtacgt cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct
     5281 actgatcttg gctggtacac agatacagtt tatgacatga agtattggac attcattgca
     5341 gaagagctac ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa
     5401 acatatgccg ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa
     5461 tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg
     5521 gatgatcttt tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat
     5581 caaatcgaag gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga
     5641 aaaccagttc caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat
     5701 caggaaatgg tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct
     5761 ggcgatcata actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg
     5821 gaagaaaaat aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg
     5881 atctctatag gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc
     5941 ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga
     6001 ctgcatactt tctttaaatc cctctaattg attttcaact acataattac gcataaatag
     6061 aactgtttct ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg
     6121 ctgctgatcc attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact
     6181 ttgggtgact gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa
     6241 tcgactcgga aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt
     6301 ataaaggtca tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg
     6361 atctggaagc gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt
     6421 gtttttgaaa taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg
     6481 catggaaagc tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat
     6541 gattgcttga gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta
     6601 aacatggtaa attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa
     6661 acaaaggggt gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta
     6721 gctgttttta tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct
     6781 aagttactac agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca
     6841 attggtattt acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg
     6901 attttctctg caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga
     6961 aagatccaga cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt
     7021 cgtctaacaa atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt
     7081 tttcgaattc cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa
     7141 ttatggtgga tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt
     7201 acaaggatgg gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt
     7261 gcaaaagaaa acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa
     7321 ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca
     7381 ttattttctg tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg
     7441 atcttttttg tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca
     7501 ttcatgaact acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg
     7561 atgacttcaa gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca
     7621 gatcttactt atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat
     7681 gtttcttttc gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc
     7741 aaacctggag aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca
     7801 caattgatcc ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat
     7861 cttcgtcaag taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca
     7921 attttattct caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa
     7981 aaagagatgg aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct
     8041 gatcgttacg aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa
     8101 cgtttatcca ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt
     8161 acgagtgcac tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg
     8221 aagggaacaa cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc
     8281 atcttagtat tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct
     8341 ggaaaccgtg tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga
     8401 ctgatctaat aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct
     8461 ttttctttat ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat
     8521 acatcgggga agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag
     8581 acgataaaag caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta
     8641 gtgcagccaa ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc
     8701 gaatgcgtat cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc
     8761 atcaagacgg tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt
     8821 tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca
     8881 tgatgttccg ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg
     8941 ttttgatagc tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg
     9001 aagtggggaa actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca
     9061 caaatggctt acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg
     9121 aagcaacctt caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt
     9181 cactggtcaa tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt
     9241 tggaacgttc ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt
     9301 accaaccact gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg
     9361 caagacgatt gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag
     9421 catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag
     9481 aaaaactagt cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg
     9541 ttggtccaac aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg
     9601 tcaatgatgg ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat
     9661 tacgtagaca tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag
     9721 acaatatcac attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac
     9781 aagcgaatat tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg
     9841 aagaaaataa cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca
     9901 tcacaaatcc atctttattg attttggatg aagcaacaag taatgtagac acggtaacag
     9961 aagcaaggat ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg
    10021 ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag
    10081 tcattgaaga aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt
    10141 atcataatca atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt
    10201 tttatatatt ttatgttgtt tgtttttata aggggca
//
LOCUS       DACCIQ010000087        10220 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCIQ010000087 DACCIQ010000000
VERSION     DACCIQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.089"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            207..2372
                     /gene="acm"
                     /locus_tag="IVM62_002263"
     CDS             207..2372
                     /gene="acm"
                     /locus_tag="IVM62_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP6846191.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2644..3129)
                     /locus_tag="IVM62_002264"
     CDS             complement(2644..3129)
                     /locus_tag="IVM62_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP6846192.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3609..4250
                     /locus_tag="IVM62_002265"
     CDS             3609..4250
                     /locus_tag="IVM62_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6846193.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4269..4904
                     /locus_tag="IVM62_002266"
     CDS             4269..4904
                     /locus_tag="IVM62_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6846194.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4922..5737
                     /locus_tag="IVM62_002267"
     CDS             4922..5737
                     /locus_tag="IVM62_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6846195.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6056..7492
                     /locus_tag="IVM62_002268"
     CDS             6056..7492
                     /locus_tag="IVM62_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6846196.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7681..10128
                     /locus_tag="IVM62_002269"
     CDS             7681..10128
                     /locus_tag="IVM62_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6846197.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         3566 a         1672 c         2202 g         2780 t
ORIGIN      
        1 gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag ttttttggca
       61 tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa aagattggaa
      121 atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag gttttatttt
      181 tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt
      241 tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca
      301 gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag
      361 gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt
      421 atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa
      481 tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc
      541 aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg
      601 agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga
      661 cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta
      721 gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt
      781 ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa
      841 tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg
      901 aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccggcg
      961 ctacatttaa tttttcagta actgataatt acattgaaat aactatacca aaaaactttg
     1021 tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa
     1081 attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag
     1141 aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta
     1201 gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga
     1261 ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga
     1321 cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa
     1381 aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat
     1441 tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg
     1501 ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt
     1561 attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa
     1621 aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata
     1681 gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc
     1741 cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca
     1801 tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga
     1861 cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg
     1921 aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa
     1981 tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata
     2041 acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa
     2101 caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc
     2161 aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc aattatatta
     2221 gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa
     2281 gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact atctgtgctg
     2341 ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt tgttaaatat
     2401 gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca tttttatata
     2461 tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa gaatactcgt
     2521 attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa gaatttctga
     2581 tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa aactactcta
     2641 tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct tttcaaagtc
     2701 ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta cggtatcttg
     2761 acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt gcatcaatgc
     2821 taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt cttcagaaac
     2881 aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg cgtgcttttc
     2941 atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata atagattttt
     3001 tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg caggatgatc
     3061 gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt gttcggttgc
     3121 acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa gagttgacaa
     3181 atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg aaaagaagag
     3241 tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca gttaaccgtg
     3301 aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg gagcccgtta
     3361 cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg agtcggtaaa
     3421 aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac tttttgtttt
     3481 catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg
     3541 aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg
     3601 aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa
     3661 cattgaaatt atttttattt accttgattg gctcattacc attagggatt gttttagcat
     3721 taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta
     3781 tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg
     3841 ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt
     3901 attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag
     3961 cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga
     4021 caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct
     4081 tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg
     4141 acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc
     4201 taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag
     4261 gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca
     4321 aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg
     4381 gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat
     4441 tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag
     4501 gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct
     4561 tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat
     4621 tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg
     4681 gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg gtattggctt
     4741 atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc
     4801 tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag
     4861 aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga
     4921 gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg
     4981 ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt
     5041 tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac
     5101 aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt
     5161 tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat
     5221 atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta
     5281 tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca
     5341 aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa
     5401 acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt
     5461 taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt
     5521 atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg
     5581 ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa
     5641 aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga
     5701 taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag
     5761 acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg
     5821 gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt
     5881 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact
     5941 cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg
     6001 tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga
     6061 acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta
     6121 tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt
     6181 acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca
     6241 tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc
     6301 tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc
     6361 tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt
     6421 atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca
     6481 gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt
     6541 ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc
     6601 agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa
     6661 tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg
     6721 accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc
     6781 tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa
     6841 atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc
     6901 tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca attactatca
     6961 aacggcgacg aatgcattca atccattaaa cggagttggt gcaggaaaaa tgaatacgac
     7021 aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa
     7081 tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg
     7141 actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg
     7201 agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt
     7261 acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta
     7321 ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg
     7381 atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga
     7441 cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata
     7501 cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga
     7561 aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca
     7621 aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa
     7681 atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa
     7741 aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct
     7801 gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc
     7861 attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg attttggatt
     7921 gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc
     7981 ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg
     8041 gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa
     8101 gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc
     8161 ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc
     8221 ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga
     8281 gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa
     8341 gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct
     8401 gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat
     8461 actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca
     8521 atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca
     8581 ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc
     8641 tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt
     8701 gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa
     8761 aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa
     8821 gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta
     8881 agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg
     8941 atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc
     9001 agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta
     9061 gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg
     9121 cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt
     9181 atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg
     9241 aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg
     9301 aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat
     9361 gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag
     9421 aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat
     9481 gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta
     9541 gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg
     9601 gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca
     9661 agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga
     9721 ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc
     9781 atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat
     9841 tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca
     9901 caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt
     9961 tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa
    10021 ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt
    10081 gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat
    10141 gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa
    10201 atggtttttt tccatctttt
//
LOCUS       DACCIQ010000088        10140 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCIQ010000088 DACCIQ010000000
VERSION     DACCIQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.090"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..769)
                     /locus_tag="IVM62_002270"
     CDS             complement(<1..769)
                     /locus_tag="IVM62_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP6846198.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="IVM62_002271"
     CDS             complement(974..1324)
                     /locus_tag="IVM62_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846199.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="IVM62_002272"
     CDS             1919..2365
                     /locus_tag="IVM62_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6846200.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="IVM62_002273"
     CDS             2989..3981
                     /locus_tag="IVM62_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6846201.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="IVM62_002274"
     CDS             3997..4881
                     /locus_tag="IVM62_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6846202.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="IVM62_002275"
     CDS             4930..6537
                     /locus_tag="IVM62_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6846203.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="IVM62_002276"
     CDS             6538..8022
                     /locus_tag="IVM62_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP6846204.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            8199..9191
                     /locus_tag="IVM62_002277"
     CDS             8199..9191
                     /locus_tag="IVM62_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6846205.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            9243..10112
                     /locus_tag="IVM62_002278"
     CDS             9243..10112
                     /locus_tag="IVM62_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6846206.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3202 a         1612 c         2178 g         3148 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa ttatttaata ggttttgaag gataatgttt ttagaaaaga acagatagct
     8101 agatttccca tggatttgct attagtattt tgaaaaaatt agtaatttat gacaaaattc
     8161 attatactaa acttgtgcag aaatgtgagg gatcaactat gaaagcaaca atgaaagatg
     8221 tcgcaaaatt agcaggtgtt ggagtaggaa ctgtttcccg ggtattgaat aacggttctg
     8281 ttaaagcttc tactagagaa aaagttgaag cagcaatgac agagttaaac tatcaaccag
     8341 atttttatgc ccgggggtta aagatgaagc atacgaacac ggttgctttg attctaccca
     8401 cggtatggca tccatttttt ggcgaatttg cctaccatgt agaaacagca ttagaaagac
     8461 aaaactacaa gctttatttg tgtaactcca gtggtaatag tgaaaaagaa caagaatata
     8521 tccagatggt cagccaaaat aaagtagacg gcatcattgg aattacttat tcagatatcg
     8581 atgaatacat ttcgagtaat ctgccttttg ttagtattga ccgttatttc actgaagagg
     8641 tgatttatgt gaccgctgac aatgcggccg gtggacgttt ggctgcaaat gaactttatc
     8701 gccgaggatg tcagaatgta gcatatatcg gtgggtacca agaaacaccg aatgaaacga
     8761 agagtcgtcg aaaatatttt gaaatcgagt gccgtcaact ggggatttcc tataaagcgt
     8821 tagatatgtt agaaccttta gaccatctct cagaacgtgt aacaaatttt ttgttagaga
     8881 atcccgatat cgatgggatt tttacgatca acgatccaat ggcattagct gtcattgata
     8941 tgcttgaaaa aatagggaaa aacgtaccag aggatgtcca agttatcgga tttgatggtc
     9001 aaaagatgac agaaggagat ccttatctag tctcaacgat tgcccaacct acaaagcaaa
     9061 tggcggaagt ggcagtggca aaattaattg ctgtcattaa tggagagaca atagaaaaac
     9121 gtaccgtatt gccgattgaa tttggcgaag gtgacacaac gaaaccttta gtgagaaacg
     9181 attttgctta gtgcaaatac gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata
     9241 aaatgttata tggtggaata gaagccggtg gaacaaagtt tgtctgtggg attggtaatg
     9301 aacaattaca agtgatagaa cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg
     9361 agcaagtgtt tgatttcttt gataagtaca aaggaaatat cgcatcaatt ggtgtaggag
     9421 catttgggcc tattgatatt caaatcgaat caagaactta cggatatatc accaacacac
     9481 cgaagttagc ttggcaaaat tatcattttg taggagcttt ggaaaacgcc ttgtcggttc
     9541 ccatctattg gactaccgat gtaaatgctg cttgttatgg agaatatatg gcagggtatg
     9601 gaaaaggtaa aaatagtgtt ctgtattaca ccgttggtac aggtgttggt ggtggtgctg
     9661 tagtggatgg gaaaatagtc gcaggtttta gtcatcctga aatgggacat atgctcttac
     9721 aacgtcataa tgaagatcaa tttgaaggaa tctgtccttt tcatcatgat tgtttggaag
     9781 ggttagcatc aggacctgcg attgaaaaac gctttggaag aaaagcccag actatctcat
     9841 ttgatcatcc ttattggacg attgaggcag attatcttgc tcagtgtgtg tataacacaa
     9901 cgttactata tgcaccggaa gtaataattt taggcggggg tgtaatgaaa cagccgcaac
     9961 tactcgagtt gattcgccaa tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta
    10021 ctctcgaaga atatctacta actccaaggc ttgctgacaa tgcgggcttg attggctgcc
    10081 tagctttagc aataaagaaa ctcaatcatt agaatagtat ttattagaac tattcataac
//
LOCUS       DACCIQ010000089        10130 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCIQ010000089 DACCIQ010000000
VERSION     DACCIQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.091"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            119..625
                     /locus_tag="IVM62_002279"
     CDS             119..625
                     /locus_tag="IVM62_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP6846207.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="IVM62_002280"
     CDS             642..1541
                     /locus_tag="IVM62_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP6846208.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="IVM62_002281"
     CDS             1628..2512
                     /locus_tag="IVM62_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6846209.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="IVM62_002282"
     tRNA            complement(2704..2775)
                     /locus_tag="IVM62_002282"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="IVM62_002283"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="IVM62_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP6846210.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4372..5409
                     /locus_tag="IVM62_002284"
     CDS             4372..5409
                     /locus_tag="IVM62_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP6846211.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="IVM62_002285"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="IVM62_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6846212.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="IVM62_002286"
     CDS             6561..7751
                     /locus_tag="IVM62_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP6846213.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="IVM62_002287"
     CDS             7855..8610
                     /locus_tag="IVM62_002287"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP6846214.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="IVM62_002288"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="IVM62_002288"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP6846215.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1869 c         2099 g         2614 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagctgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggtggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 tttttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga
//
LOCUS       DACCIQ010000090        10080 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCIQ010000090 DACCIQ010000000
VERSION     DACCIQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.092"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(543..767)
                     /locus_tag="IVM62_002289"
     CDS             complement(543..767)
                     /locus_tag="IVM62_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846216.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            967..3735
                     /locus_tag="IVM62_002290"
     CDS             967..3735
                     /locus_tag="IVM62_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846217.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3817..4236
                     /locus_tag="IVM62_002291"
     CDS             3817..4236
                     /locus_tag="IVM62_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP6846218.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4248..4718
                     /locus_tag="IVM62_002292"
     CDS             4248..4718
                     /locus_tag="IVM62_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6846219.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4735..5514
                     /locus_tag="IVM62_002293"
     CDS             4735..5514
                     /locus_tag="IVM62_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6846220.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5501..6322
                     /locus_tag="IVM62_002294"
     CDS             5501..6322
                     /locus_tag="IVM62_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6846221.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6339..7388
                     /locus_tag="IVM62_002295"
     CDS             6339..7388
                     /locus_tag="IVM62_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846222.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7401..8405
                     /locus_tag="IVM62_002296"
     CDS             7401..8405
                     /locus_tag="IVM62_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846223.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9038..10057
                     /locus_tag="IVM62_002297"
     CDS             9038..10057
                     /locus_tag="IVM62_002297"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6846224.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3384 a         1657 c         2099 g         2940 t
ORIGIN      
        1 tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact tcttttttaa
       61 tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt gagagaaatc
      121 taattgattt tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt ttattgtggg
      181 tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca gattctatca
      241 ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg agattttgat
      301 ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg ttttcgtaat
      361 caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg acaacagtac
      421 ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat aaattgttgt
      481 tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga aatctgtgaa
      541 tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat gaataaaaat
      601 cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc tgtcatagcc
      661 atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat ttctcgaaaa
      721 gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga atagtcatag
      781 cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt
      841 tttagaataa aaattataag ggataagtag gctgctgtct ataataagat atatttttca
      901 attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg aggaggggtg
      961 aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc ctttttcgat
     1021 ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt tggggtaaaa
     1081 agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact agtcaaaatc
     1141 aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt ttttaagtta
     1201 cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt tttcgcgcaa
     1261 ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga aagtatcgaa
     1321 caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat tacaggcgaa
     1381 agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct gcttcatgat
     1441 ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc agataatcct
     1501 gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg tgcagaagaa
     1561 aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga cgaggtacat
     1621 cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg tgtgatttat
     1681 cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt tgctacgact
     1741 gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat ccgaatcgaa
     1801 ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta ctcttttttt
     1861 ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc tttgtcactt
     1921 ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt caaagtttca
     1981 gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac gatccatcat
     2041 ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca aggaatctat
     2101 attacgcaag accagacctt agagcaatta atggagaagc aacagcccga acagcagcga
     2161 atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg tctgctatat
     2221 tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt gctgtttgag
     2281 accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat ccgagagacc
     2341 tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt ctatgccttg
     2401 agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca tgatattatc
     2461 caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag ttatcactat
     2521 gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac aagtatggat
     2581 cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa acgactgcca
     2641 aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa tgtagtaaat
     2701 cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt cacaccacag
     2761 cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa tggattgatc
     2821 atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa acagttatcc
     2881 gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt aggggaatgc
     2941 ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca gatccagcca
     3001 gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac tacttgcttc
     3061 actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt acctacggct
     3121 tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa aaacagtgac
     3181 ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc tgatatcacc
     3241 aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc tgtactagct
     3301 gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca gcgcatcatt
     3361 cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac agagaaggtg
     3421 atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg aagcatcagc
     3481 aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg attgatccga
     3541 caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca aaaagaaggg
     3601 ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt catgatcccc
     3661 aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt tctctcgata
     3721 gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc aacttgcttt
     3781 ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat tatcttagct
     3841 tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta tgggaaaaat
     3901 cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc ggaaacagta
     3961 aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga tctttttggc
     4021 ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta tctcgttgcc
     4081 ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga agtagagaca
     4141 actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt ttgtaacgag
     4201 cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg attgtattga
     4261 caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca caaacaatag
     4321 gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata cgaaagacaa
     4381 cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt gatgactcga
     4441 ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta gtggaatcga
     4501 tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc aacttaggag
     4561 ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt ttaccagaag
     4621 aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc cgtcaagtac
     4681 cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac ctcaatgatt
     4741 gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata tgcactaggg
     4801 acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt gttaggtgat
     4861 gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg atcgttctca
     4921 gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac ggcttttgct
     4981 attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat tgctacgttg
     5041 acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa ccataaagca
     5101 gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt gcttgccggt
     5161 atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt gggaagcaat
     5221 acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt gtcagtagct
     5281 acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat caacaaacaa
     5341 gttgcaccat attttttctt aggttttgct atagcttctt acttagagat tcctgtgacc
     5401 ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat gaatgtccgt
     5461 caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt ctgagatcaa
     5521 aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt ttcaaatgga
     5581 gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa tgattccgat
     5641 tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac ggcatctaga
     5701 gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg ctgcaatgga
     5761 ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta tcaaaacatc
     5821 tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt tgcgattgat
     5881 tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc ctatcttgtt
     5941 cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag gttggggata
     6001 tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg aaagtttgac
     6061 attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca cgatgatcac
     6121 catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc aaagtatttt
     6181 tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt tttatctatt
     6241 gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg gaatttttgg
     6301 ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg caagattata
     6361 tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt tcgacaagag
     6421 aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct tataacgctt
     6481 gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat attcaagaac
     6541 ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg attgctgtgt
     6601 cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa aaacaagggt
     6661 tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa gctgcacaaa
     6721 ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc tattctgcga
     6781 ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat aaaatcagca
     6841 aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat ttaccagcga
     6901 ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt ttagcacagc
     6961 gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca gagacgaaat
     7021 tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat atgcacggcc
     7081 catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa agtgtaaata
     7141 aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag actctcacca
     7201 ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa tgtgatgaat
     7261 atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa accgcagtag
     7321 ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc ttgaaaagta
     7381 aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc aaagcaataa
     7441 aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt tatgcggaaa
     7501 aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg atgcaagctg
     7561 tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct gcagaatatt
     7621 acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc tctgttaccg
     7681 gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga gcgacactga
     7741 ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt gtggtcactt
     7801 atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga ttgatgtatt
     7861 tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt tatttaccaa
     7921 cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt gcagaaaaac
     7981 atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga gctgtttatt
     8041 catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct atccatgctg
     8101 ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc acattatttt
     8161 taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg ccaaaaattt
     8221 gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc agtgaaaaat
     8281 atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc atcgatgctc
     8341 atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt cagtttgatt
     8401 attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat aactgctccc
     8461 tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt gcattatcta
     8521 cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt ttgttatgca
     8581 atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa cgagttgcgc
     8641 cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc tatgtcaacc
     8701 gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt tcaatctgtc
     8761 tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct aatagtaatg
     8821 ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat aacgagtgta
     8881 aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa acgataaaag
     8941 aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa tgtatactat
     9001 aatggaaatt atcaattatt tatttggagg aatatccatg aaacaaaaaa tcgcagttct
     9061 tggtcctgga tcttggggca ctgctttagc acaaacattg gcagaaaacg ggcatgatgt
     9121 ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc aataagtatc atacaaatca
     9181 gcatttttta cctgatttga cgatcccaga atcgattatc ggatataaag aattggcaga
     9241 agcagtggat aacgcagatg ctattttatt tgttgttcca acaaaagcga ttcgttcagt
     9301 cgcacaggaa cttattggta aaatgaatac taagccaatc attatacatg caagtaaagg
     9361 actagaacaa gatacgcata aacgaatctc agaagttttg gctgaagaaa ttccagaaga
     9421 aaaaagacag gcaattgttg tattatctgg tcctagccat gctgaggaag tggcagtaca
     9481 tgatatcaca acaattacag ctgcaagtat tgatcaaaaa gcagcagcct atgtacaaaa
     9541 actgttcatg aatgattatt tcaggattta tacaaataat gatgtgattg gcgtagaaac
     9601 tggtgcagca ctgaaaaata tcattgcaat tggtgccggc gctatccatg gattaggatt
     9661 cggagatgat gcaaaagcag caatcatgac tcgaggattg gcagaaatca gccgtcttgg
     9721 tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg gggacttgat
     9781 cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt taggacaggg
     9841 acataagctg gaagaagttc tagaaaacat gggaatggtc gttgagggtg tggcaacgac
     9901 aaaagcagca gtagagctag ctcaacagct aggtgttgag atgccaatta ctcaaacaat
     9961 ctataatgtt ttgtataatg gagaagatat caaaaaagca gcaaaagaaa tcatgttacg
    10021 tgacggaaaa atggagaatg aattttctct tcgttaaata gatgatcaat agttaatcgt
//
LOCUS       DACCIQ010000091         9975 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCIQ010000091 DACCIQ010000000
VERSION     DACCIQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.093"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(200..2320)
                     /locus_tag="IVM62_002298"
     CDS             complement(200..2320)
                     /locus_tag="IVM62_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6846225.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2550..3728
                     /locus_tag="IVM62_002299"
     CDS             2550..3728
                     /locus_tag="IVM62_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP6846226.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3744..4502
                     /gene="aroD"
                     /locus_tag="IVM62_002300"
     CDS             3744..4502
                     /gene="aroD"
                     /locus_tag="IVM62_002300"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP6846227.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4525..5010
                     /gene="ybaK"
                     /locus_tag="IVM62_002301"
     CDS             4525..5010
                     /gene="ybaK"
                     /locus_tag="IVM62_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP6846228.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5081..6334)
                     /locus_tag="IVM62_002302"
     CDS             complement(5081..6334)
                     /locus_tag="IVM62_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6846229.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6451..7800
                     /gene="gdhA"
                     /locus_tag="IVM62_002303"
     CDS             6451..7800
                     /gene="gdhA"
                     /locus_tag="IVM62_002303"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP6846230.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7912..9258
                     /locus_tag="IVM62_002304"
     CDS             7912..9258
                     /locus_tag="IVM62_002304"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP6846231.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9566..9952)
                     /locus_tag="IVM62_002305"
     CDS             complement(9566..9952)
                     /locus_tag="IVM62_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6846232.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3194 a         1804 c         2042 g         2935 t
ORIGIN      
        1 tagaaaatga atatgaataa agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag
       61 aaaatatttt gtaaaaagca agaggttgtg aagaagctgc cctattctat taccgtagat
      121 gcaagctttt gaacactgtt tattcgaaaa gaggagcagc gacaataatt ttgtcgctgc
      181 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
      241 acgtttgcgt tttataaaga tcaacagttg attgtccgtt gtgttcacta aagacactag
      301 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
      361 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
      421 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
      481 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
      541 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
      601 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
      661 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
      721 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
      781 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
      841 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
      901 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
      961 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
     1021 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
     1081 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     1141 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     1201 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     1261 cactaatgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     1321 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     1381 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     1441 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg agaccattct
     1501 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     1561 cattttttcc tctagcgagc ccgaacaaat cgtcatttgg tgctgcataa tgttctttta
     1621 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     1681 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     1741 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     1801 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     1861 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     1921 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     1981 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     2041 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     2101 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     2161 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     2221 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga
     2281 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     2341 ttcttaatca cagaagtgtc ctgaaaccat gttacacagc tcacttattc tcatttattt
     2401 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     2461 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     2521 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     2581 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     2641 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     2701 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     2761 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     2821 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     2881 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     2941 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcata ggaggctatg
     3001 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     3061 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     3121 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     3181 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     3241 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     3301 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     3361 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     3421 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     3481 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     3541 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     3601 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     3661 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     3721 accattagaa ggagcgatgc agaatggttc gggtaaaggg attgaatata ggaagcggta
     3781 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     3841 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     3901 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     3961 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     4021 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     4081 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     4141 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     4201 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     4261 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg
     4321 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggttg
     4381 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     4441 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     4501 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     4561 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     4621 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctcg tatatttaaa
     4681 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     4741 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     4801 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     4861 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     4921 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     4981 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     5041 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     5101 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     5161 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     5221 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     5281 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     5341 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     5401 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     5461 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     5521 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     5581 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     5641 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     5701 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     5761 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     5821 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     5881 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     5941 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     6001 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     6061 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     6121 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     6181 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     6241 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     6301 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     6361 tatctgaaga aagaaaacag aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat
     6421 gagggaagat aagcaaagga gcgtggaacg atgacaaacg caacagaata tgttcaagca
     6481 attcaagaaa agctacatca aaaggatcaa gggcaagaag aatttttaca agcaatcgat
     6541 gaattcatgc ccacggttat gtcttttttg gacactcatc ccgaatatat cgaaaagaat
     6601 atcttagaac ttttaacgga accagaaaga gttattcaat ttcgagtgcc ttggcaagat
     6661 gatgaaggaa actggcgagt aaatagaggg taccgtatcc aatacaactc agctatcggc
     6721 ccttataaag gtggattgcg cttccatcct agtgtcaatc taagtatctt gaaatttttg
     6781 gcttttgaac agattttcaa gaatagttta accggtctgc caatcggtgg aggaaaaggc
     6841 ggaagtgact tcgatcctaa aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt
     6901 ttcatgctgg aattgtcaaa acatatcggc ccatcgattg atgttcctgc gggagatatc
     6961 ggtgtaggag ctagagaaat cggctatttg ttcggcgaat ataaacgatt gaaaaaatat
     7021 gatactggtg tgttgacagg taaacctttg gatttttggg gaagtaagat acgtacggaa
     7081 gctactggtt atggattagt gtattatgtc aaacatctat tgaacgaaga aaaagattca
     7141 tttaatcaaa aaacagtttt tgtttccgga agcgggaatg tagccattta tgcaatcgaa
     7201 aaagtgcaag agctaggcgg gaaagttgtt acatgttcag attctagcgg gtacatctat
     7261 gatccagaag gaatagatgt gaaactattg aaagaagtca aagaaatcaa tagacaacgt
     7321 ttgacggaat acgcaaacca acgtccttct gctatttatc atgctggaga atctgtttgg
     7381 acattgaaag aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa
     7441 agcttggccg aaatcttagt agaaaatggg atcaaaatcg tagcagaagg cgctaatatg
     7501 ccatgtacag taaaagctgt ttctgttctt catgaagcag gcgtgtggta ttgtccagga
     7561 aaagctgcta atgctggagg agtagcagta tctgctttag aaatgagtca gaacgtccag
     7621 cgattgactt gggaaagaga gcaagtggat gagcagctgg atcaaatcat ggaaacgatt
     7681 taccatacgt gtcgccaaac agcagccgag tacggcgacg aaaaaaatct gcttcttgga
     7741 gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg cagcagaagg tttagtttga
     7801 aaaatgaaac tttcttggaa atattgtcag cattttgttt atttgcttga aaaataataa
     7861 aaaaaaacgt aaagtattac ctgtagagta aacctataag gaggagaaca aatggcacac
     7921 attcattttg actattcaaa agtagcaccg tttgtcaacg aacaagagtt agggtacatg
     7981 caaagccaag taacagcagc ccacaacgaa ttacgggaag gtactggcgc aggaagcgac
     8041 ttccgcggct ggatcgattt accaacgaac tatgacaaag aagaatttgc acgtatcaaa
     8101 gaagcagcta aaaaaatcca atctgattct gaagtattag ttgttatcgg tatcggtggt
     8161 tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc acacattctt caatttatta
     8221 gatagcaaca caagaaaagc accacaaatc ttctttgccg gaaattcaat cagctcaaca
     8281 tatattgctg acttgatcga agtaatcggt gatcgcgact tttcagtaaa cgttatttca
     8341 aaatctggaa caactactga accagcgatt gcgttccgtg tattcaaaga gcttcttgtt
     8401 aagaaatatg gacaagaaga agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa
     8461 ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac
     8521 atcggcggac gtttctctgt attaactgct gttggtttgt taccaatcgc tgttagtggt
     8581 gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac
     8641 aaactagaag aaaacgaagc ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa
     8701 ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa tgcaatactt ctcagaatgg
     8761 tggaaacaat tgtatggtga atctgaagga aaagaccaaa aaggcatcta tccttcaagc
     8821 gcaaacttct caactgattt gcactcttta ggacaattca tccaagaagg acgtcgcaat
     8881 atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa
     8941 gcagaagatt tagacggtct aggctacttg caaggaaaag aagtggactt cgtaaataca
     9001 aaagcatttg aaggaacatt gcttgctcat acagacggtg acgtgcctaa cttgctagtc
     9061 aaaattccag aaatggatgc ttatacattg ggttatacaa tgtacttctt tgaaattgct
     9121 gtaggtattt caggttactt gaatggaatc aacccattcg accaaccagg tgtagaagct
     9181 tacaagaaaa acatgttcgc acttctaggc aaaccaggct ttgaagatct tgctaaagaa
     9241 ttgaacgaac gcctgtaaga taagggtttt tcgctagatt taaataatca gtgatagaaa
     9301 catgttcgct ttaaaatgaa aactggtacc catttaggta ctcaaacata ctgcatcgtt
     9361 tattcagaat aaggggctgt gacaagacgt ttgtcacagt cccttgtttg tatagaaatc
     9421 gattgattgc cttgctcttt agtttgtttt aggactttaa gaacagtgag gctgacttcg
     9481 aaggttaatt ctgttttttt gttttctaga atcagtaagc aaaaaagcac tcacttcgag
     9541 tgcttttttg cgatatattc ttagcttaag ataggcctaa tttttctttt acagatgagg
     9601 gtaccttatc gctggtaatt acttttttct ctatgatccg cttttcgctt gctttcaact
     9661 gcaagatcgt tccttcgggg taagcagaac cattggcaga gtcaccagaa gtaatgacat
     9721 cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc atctacgttg tatttaaaac
     9781 ctcttccaat tacttcccca ttatctgctt tgatatcagt ttctgcaggc attggagctt
     9841 tgactaccgc ataataatct ttacctacat aagtagactg atagtagtcc caacctttcc
     9901 atcctataaa agcaatcaat atgataacga tcaaccctaa aatttttttc atgaaagatc
     9961 tcctttttta ttttt
//
LOCUS       DACCIQ010000092         9886 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCIQ010000092 DACCIQ010000000
VERSION     DACCIQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.094"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..312)
                     /locus_tag="IVM62_002306"
     CDS             complement(<1..312)
                     /locus_tag="IVM62_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846233.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..1018
                     /locus_tag="IVM62_002307"
     CDS             434..1018
                     /locus_tag="IVM62_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846234.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1029..1274
                     /locus_tag="IVM62_002308"
     CDS             1029..1274
                     /locus_tag="IVM62_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846235.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1280..2002
                     /locus_tag="IVM62_002309"
     CDS             1280..2002
                     /locus_tag="IVM62_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846236.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2133..2876
                     /locus_tag="IVM62_002310"
     CDS             2133..2876
                     /locus_tag="IVM62_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846237.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2882..3247
                     /locus_tag="IVM62_002311"
     CDS             2882..3247
                     /locus_tag="IVM62_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846238.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3263..3793
                     /locus_tag="IVM62_002312"
     CDS             3263..3793
                     /locus_tag="IVM62_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846239.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3955..4263
                     /locus_tag="IVM62_002313"
     CDS             3955..4263
                     /locus_tag="IVM62_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846240.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            4259..4678
                     /locus_tag="IVM62_002314"
                     /pseudo
     CDS             4259..4678
                     /locus_tag="IVM62_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4815..5615
                     /locus_tag="IVM62_002315"
     CDS             4815..5615
                     /locus_tag="IVM62_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846241.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5652..5834
                     /locus_tag="IVM62_002316"
     CDS             5652..5834
                     /locus_tag="IVM62_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846242.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5945..6262
                     /locus_tag="IVM62_002317"
     CDS             5945..6262
                     /locus_tag="IVM62_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846243.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            6265..6497
                     /locus_tag="IVM62_002318"
                     /pseudo
     CDS             6265..6497
                     /locus_tag="IVM62_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6612..7091)
                     /locus_tag="IVM62_002319"
     CDS             complement(6612..7091)
                     /locus_tag="IVM62_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846244.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7211..8548
                     /locus_tag="IVM62_002320"
     CDS             7211..8548
                     /locus_tag="IVM62_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6846245.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8571..9098
                     /locus_tag="IVM62_002321"
     CDS             8571..9098
                     /locus_tag="IVM62_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846246.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9085..9648
                     /locus_tag="IVM62_002322"
     CDS             9085..9648
                     /locus_tag="IVM62_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846247.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
BASE COUNT         3711 a         1272 c         1945 g         2958 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg aacttaggga
      961 atatgtgcag aacccatata attttcaata tttagcagga agaataaatt tagtttagga
     1021 gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt gaaggtgtta
     1081 ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc attcaaaaaa
     1141 atccacaata cctattattt agtctttatg cttggattgt acaaatggtt tgggtttcca
     1201 tattgatgct gttgttttat tggatagtac gggttattct tttattgtta aacaaaagaa
     1261 aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt tatcgacagc
     1321 agcattaata acccttacag catgtggtac aactaatagt gctaataata acaaagacac
     1381 aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa caacaatgtc
     1441 ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta ataaaggtca
     1501 aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg aaaattatgg
     1561 gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg attgtccaga
     1621 ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta gttatgctcc
     1681 aacattaatg catgatatta ccataaataa accagcttac gagtattacg cagttgctgt
     1741 agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg ctgatgagaa
     1801 caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta acggcacact
     1861 atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg aaagtacaac
     1921 taaatattat atggatttgg gagcatataa attatatcaa caatcagagc ctgttatgga
     1981 cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt aaaattaagt
     2041 gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga aacacacaag
     2101 ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca aatgaagctc
     2161 atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa ggaatttata
     2221 aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta aaggcaacaa
     2281 gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca agtgaagaaa
     2341 tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa aaacatctac
     2401 tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa aaattaagaa
     2461 ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc ttaaatgact
     2521 acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac acacaataca
     2581 aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg tatgagatga
     2641 aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg aatcaagcga
     2701 ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg atcgtaaatg
     2761 aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat
     2821 caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg agatgagtta
     2881 gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa aaatacagat
     2941 gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg atcatattgc
     3001 taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga tacatcacaa
     3061 tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat atgatctatt
     3121 agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg cagagttaaa
     3181 aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga taatgcaacg
     3241 aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac aattaataga
     3301 agaagattta gagaccacag ttcagagatt agcggaacga atccattttg caaattatca
     3361 aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa tgaaattttt
     3421 ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga agaccatgat
     3481 ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag aggcaaaaga
     3541 aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga
     3601 tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa tagattttat
     3661 tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag atcatgaaca
     3721 gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata acaaggtgtt
     3781 tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac aaagaatgta
     3841 tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta tatttaaaac
     3901 agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa ctgcatgtgg
     3961 aataatagat taaaaacagg attgttatta atagttattt cctgcgctat gatgattggt
     4021 atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaatgt aatagaaaga
     4081 tgccattatg gtcaacattt ttggactggg gaagtcagag aaaatataga tcgtgaatat
     4141 gttcaaagaa ttgtttggga tgcatattct atcaaagatt atcctaaaag tttaacgagt
     4201 agattatttt acagtgaaaa agataatcaa aaactctcag atttgatgat gaaaaaagta
     4261 tgactattat tacgaaagag aaacagagtg ctatattcga ggggaaaaag cctacaacaa
     4321 aagtgactat tggggtgaca aggcgaagcg attaactgca aattttagag tgtatgaatc
     4381 agagtttata aacttgacct ttcttaatag tgtttttatt gattatgtaa ttactacgaa
     4441 acgcattgga agttattcaa gtacctacgc atccttagtt ccaatgttaa actcgatgag
     4501 ccgtttctta aaagaaagag aaaagaaaga gctacagtat atcaaagaaa tcatgccgga
     4561 ttttgaaatg cacgaatggc aaattgcttt atcagattgg aaattggcga ataaggttcg
     4621 tagaataaca ccttttcaag ccaagcgctt tttgagagct atgaaatttg tgcaataaaa
     4681 aaagccacca gacaagttga tggagactaa cacaattacc aacagttgta attgtgttag
     4741 cttacaaatt aattatggtg tttagtcaat cccaaattgt gaaatggcac caataattgg
     4801 aaaggaaaaa tgaaatgtct gaagatttac gagagctgaa gttacgaaag aagcagtatg
     4861 atcagtattt ttccggtttg ttgaataaac atattgaaga aggacttgat catgattttg
     4921 gaaaagatga atattgttta tattgcgaac gttacaatga aaaaatgtct gaatatctac
     4981 agctgaaaaa agagatacag aaattagaga aaaaagtaca tgcttggaag tataaagatt
     5041 ccatgtctgg ttatgttgaa tctcaaaata agccaagaaa aaagaagcaa gcgtatgttc
     5101 taaagatggt aaatggggaa gagttaattg tattagcgta cagtgaagaa gaggttcgag
     5161 aactcgttga aacacctgtc aggaaaaagg ttgaaacaat aaatgagata gacgacagcc
     5221 aatacaatag catctatatt gaaacaagtc atggtggata taagaaatta gcaactttcc
     5281 ttagaagttc taaagttggg ataatttcta aaaaagacat aagtgctgtt accgatagtt
     5341 tttgtacttt atctgttcct aaagcttatt tccaatacga tagtaaaaga tttgaacgct
     5401 tagagaaatt ctgtagaccg tatactgtat tgaaaactaa aaaatttaat aaaactaaaa
     5461 gagtttctat acagttaccg actggtcaaa tctcattagg tgaaaaagac tatctgataa
     5521 aagtaggaaa agagttaatt ataaaagtac cggaacactt atttaaagaa aattttgaga
     5581 tacgtgaaac cttggtatgg aaagaagaaa aatagtttgg aagacataga atggtataag
     5641 gggaaattga aatggcagta aataaaatat tgcaggtact aatcatagac gaatttggcg
     5701 gaggagaatg gtttgatgtg cagtccatag actttgaaga agaagtcgta gttatagaaa
     5761 atatttacgg cgaagtatat tcatattcgc ttgaaggtga gaatattgag gatttacggt
     5821 tgattggtga gtagaaaaaa acagaaatat caaaatggag ataaaaatta atagcatgta
     5881 aagatagtca tgcttgggtt aaaaatgtta aaattcaaaa aaatgagtca gaagggataa
     5941 aagtatgaaa agagaggaat acaaacaaag attaaacgaa ctgcttgaag aggacgaaac
     6001 attaactcat ggttcgcctg atgaaattct atacatgatt gataatatgg tgatctttgg
     6061 tggatatgaa cttggtaata ggtcagttga tcataatatt ttagaatttg atgatgtatc
     6121 atgggaagaa attcttgatt ggggaatttt ggcggtacct gaaactaaaa cctatattag
     6181 tgacactatg gttccttttt ttgaggaatt ggattataaa agacttccca aaaatgaaaa
     6241 tcatatactg ggaggaaact aacaatgtta agtgacttat ttattttact gatctatcta
     6301 ggtgtaagtg aaattattgc gtattttttg tttgtcattg ctaattatgg tatttccagt
     6361 gcaatcgttc aaatgtttag attaccagta agtgaactaa aaaaatgatg agattatcag
     6421 catatctttt aatgtttttg aatgtggtca cgttaatctt tgtagtaatc ggtgtgactt
     6481 tcgatttaat ggaatagtgg gaaatctata atggaaaaaa gagacacgaa tgaacatatt
     6541 ttttgaatca atattgttga tggtttttgc gatcagcgct taccagaaat ctaaaaaaga
     6601 gtaatgattg tttaatcatc actctttttt tgtagggaaa ttgaaatatg tggagcttgt
     6661 attggtttag ttaggattgc ttggctataa ggagctagtt ctttatcaaa accatcaaag
     6721 taggttttca gtagcgattt aactgatcgc tcatcttgtt tgatttggat aatttcagca
     6781 tccaaatcgg ttgtgagatc aggagctttt gatgcgatta cctgattttt tttatcttcc
     6841 aactctctta ggctgtttaa caagttgtaa aaagaatcat tttgattgtt cattccaacg
     6901 tgagttgcaa ttttaacaga agtttcgtcc gtattgtcat caaaagaact cattggaata
     6961 tcaagtagta acgaggatgt ttcttttact gactttttct ttacagcatt ttttgttttt
     7021 ccatcttttt tgctctttac tcgagatgtt tttggagcta gtttaggtaa atcacttgtt
     7081 ttatcagtca tttattagct tttcctttct attaatttat gttattatat tatcataata
     7141 acataaaaaa gtacactttt tgggggcgta gttattgaaa aaatctaaga gaaagattgc
     7201 cacattgctt atgttaatgg tgcaatttac agggattgca atgagtgtaa tcacaataaa
     7261 tccgcaagtg atacatgctg atgggatagc tacttatgat gcacaattta actgtactac
     7321 tgaaggtagt gggtcaggaa cagatagcga tggaaacgat actggaagtg gcgctgtaac
     7381 aggtgatgta gccaaaaata ttgaagatat ttacaaatac atgaatggag aatatggctt
     7441 atcaggtgct agtattgcag gtattttagg taactggaaa caagaatcag gtattgatcc
     7501 tcgagcagta gaagggtatt acggagatgg acagagagaa gttgacttca tcaaacaatt
     7561 tactgatggt ctgatgggta ttggctatgg tcaatggaca gctgggcgac atacagcatt
     7621 agttgattgg tctaagaaaa ataatggcgg ttcttggtgg actacagaag cccaattgga
     7681 ctttatgttc aaaggtgatg ggggattcgt aggaatatta aaagattatg ctttaaatgc
     7741 ttctgatgat gtcgtagaaa atgcagtaac ttttcataaa aactgggaaa tttcagctga
     7801 ttccgaatca gctgtaatag ctgtacgtgg tgaaaatgca aaagaaatct ggaagtacat
     7861 gaaaagcaag ggtatggacg gtgcaaaaga tgaaagtaag atcaataaaa tatccggagg
     7921 ttcaagtacc ggagatggat cgaataattc atcaagttca gcatcaggaa cagtagaaga
     7981 tgcctgtgca actacggatg acgctaattc tggttctgga tcaattggga aagaaggaaa
     8041 aattaacggt aaaaaaggtc aagtaatagc gaatatgact tatgaagaag ctttgaaaaa
     8101 atatgggaaa gcgatcacgc taccaaaatt caaagaacca gattggtcaa cttcaccttt
     8161 taacggaata tatcaaggtc agtgtacaga gttgacgtgg gcttatatga gccaattata
     8221 cgatgggaat caaccgacta ctggaaatgg tggttatgta tgggaaagct acaaatcggc
     8281 aggagcaaaa ataacgaaca aaccaatcgt aggttatggg tttagtgctt cggatggatg
     8341 gttatgggct gttggagatg cagggcatac tggtgtagtt atagcggtgt ttgaagatgg
     8401 atcatttcta tgtgctaact ttaatgttcc accaacccaa gctccaacaa ggggagtttc
     8461 ggtaacgttg attgatggag tggacggatc agataatgtg catttcttta gcggtgtagg
     8521 aaaagctaaa tttgaaatcg aaaaataaat tggaatgaag gacgatatag atgaatagta
     8581 aagtgaaaga tacaattatc atttctttgc tttctggtat gttattagca ctgcttgttg
     8641 gaatcctatt cttcaataag cagaatcagg acatagaagc tgaaaaacac agtgtgcata
     8701 ttgaagataa gctgacattc tttaaagagg ttaaaccaaa tgaggtggat ggattacgga
     8761 aagatttaga aaatgatatt caagaaatgt tgaatggatc agttcaaaat aagtttgcag
     8821 atcaatcagg tgaagaattt ttgagaaaac tatttattgt aacaggtaaa ccaccaatta
     8881 ctgataagag tagtgagaaa gaaaaaatcg agtactataa gccttttact tttaagctaa
     8941 caaactttgg aattaagaca tctagtagtg ggttgtatga agcattgacc gattttgaag
     9001 ttcaatacaa tggaaaaaaa gtgtatgata ccacattaac catcacacta aataatgata
     9061 agaaagtgac tgggggtgct ttttatgcaa aatcatgaaa tcactactgc taaaaaggcg
     9121 atcggaatac tttccataat tgtgatcctt tgttgtgtgg tactatttca aaagaaaaat
     9181 gcttatgata ataatgtcaa agaattggaa gaccttaaaa gtaaacaaat cgaagtagtc
     9241 aagaataacc agaaagtgaa agctatgcag gaagctcaat acaaagaatt gggaaatgct
     9301 gaaattaagg aacaaagtag gaaattcttt aatttatttt acaactggaa ttcatgggaa
     9361 aaatatacga agaatatgaa ggaaatacaa aaagaatttc ctcagatagt aaagtctagt
     9421 cctgttgata tttctggcac gatggtaggc actggtaata gtccaatatc tagctatact
     9481 gctgattact atacaacaaa tcaaaaaggt aaaatggttg aattgattac acagacaaga
     9541 aatactccaa caacagcaac aacaacaatt tggcgtggag taacaagcat aggagaaaat
     9601 caattgtttt atttggagga tttagatagc tatgtaagag cagagtaaaa taaaaaaaga
     9661 gctaacggta ccaacgttag ctctttgtgc tgacgatgat aacaaaacct accgaaaaag
     9721 gattgtttca tcacaaacac aaacagaaaa gaattgataa aaaacccgaa cctcagaatt
     9781 tttattaatc aattcattta gattataata aattgagctg aacattacaa tacgtatatg
     9841 ttgtaaaacg taaaaaaagt caaaagtaaa tactttctta aaaagt
//
LOCUS       DACCIQ010000093         9678 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCIQ010000093 DACCIQ010000000
VERSION     DACCIQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.095"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(45..1112)
                     /locus_tag="IVM62_002323"
     CDS             complement(45..1112)
                     /locus_tag="IVM62_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6846248.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1132..1611)
                     /locus_tag="IVM62_002324"
     CDS             complement(1132..1611)
                     /locus_tag="IVM62_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP6846249.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1628..2089)
                     /locus_tag="IVM62_002325"
     CDS             complement(1628..2089)
                     /locus_tag="IVM62_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6846250.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2103..3353)
                     /locus_tag="IVM62_002326"
     CDS             complement(2103..3353)
                     /locus_tag="IVM62_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP6846251.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(3731..5182)
                     /locus_tag="IVM62_002327"
     CDS             complement(3731..5182)
                     /locus_tag="IVM62_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6846252.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5503..6267)
                     /locus_tag="IVM62_002328"
     CDS             complement(5503..6267)
                     /locus_tag="IVM62_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6846253.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6295..6993)
                     /locus_tag="IVM62_002329"
     CDS             complement(6295..6993)
                     /locus_tag="IVM62_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6846254.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7005..7784)
                     /locus_tag="IVM62_002330"
     CDS             complement(7005..7784)
                     /locus_tag="IVM62_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP6846255.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7800..8744)
                     /locus_tag="IVM62_002331"
     CDS             complement(7800..8744)
                     /locus_tag="IVM62_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6846256.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8790..9569)
                     /locus_tag="IVM62_002332"
     CDS             complement(8790..9569)
                     /locus_tag="IVM62_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846257.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2784 a         2021 c         1485 g         3388 t
ORIGIN      
        1 ataagttcat tacataatcg aaacattttt tattttcctt gtcatcactc cttgctccct
       61 ttttgataca gatttaaaat tctatcaaag aaatttccat cgttaaattt cgttattgcc
      121 aaatccttcg aacaacgtga aaattttacg tatttatctt tatcattgat ttttagtatt
      181 gcagatgtca acgctgatac gtcaccaggt tgaaacaaat atccattttc tccatcacgt
      241 actgctgaag aaatatctcc tacatctgta gcaattacag gcattccata agaaatcgcc
      301 tctagaatag atataggcaa tccctcgtta taggacggta aaatcatcac atttgatctt
      361 ctatagcact cctccttttt ttctcccaca acccaacctg taaattcaaa atacgattgc
      421 aaattatatt cttctatcaa ttctttgagc gatatttctt catcacctgc tccagcaatc
      481 tcaaaaataa actttttttc tggatattgt tctgcaatgt ttttaactgc ttctaccaaa
      541 tcgtgtacac cttttcgctt tatcaaaact cccataaaca gaaatcggca catttttgaa
      601 ttccacttta tggtatattc tggaactaca atagaatttt ctacaacaac agttttacaa
      661 tttggctcaa tttctttaat tatcttcttc catttttctc caagtacaat cgtggaatca
      721 gactctctta acagcaagcg aatctgattt tgttttttta cagaaacact atcataccat
      781 tttttaaatt ccgatccatg aagatggatg attacaggaa tatttaataa tctaaaccac
      841 ttattcagca catatttcct actaaaactc cctctgtacg acatgtgcat atgaataata
      901 ttaggtttac tcaataaaga gatacctata attttagcaa atgcatacat aaaatataaa
      961 gatttaaata ttgcatttcc acccttataa ctcggtataa aagtcatctc gatattcttt
     1021 ttttcccaat taaaattgag catttgtgta attatacttg taattccacc ttttaccgag
     1081 ctgtcattac caatcataag tactctttgc ataacgcttt actccttata tttatatttt
     1141 ttctattaat acctctatcg aattaataat cgtttccgtg ttagatacat acttgttaaa
     1201 catccgaact cttgtttctt taacggcatc ttctaaattt tctaattcat aacaaggata
     1261 gataatattc atctcatcaa attgtctaat taactgcagt tggtgatcat cgacatgctc
     1321 accatactta gccaatcgag gaacagcaat gaccctttta ccttttttta cggccccaat
     1381 tatagcacct gtacctccat gagtaatcac catttctgct ttttgcatag tttcagtaaa
     1441 ctcatcccta tttagaaatg ctttatagga ataatttagt ggtttataat cactataacc
     1501 actctgtgca aatgtacttt cgattattac tcctttttca attaaatcat caatagcttt
     1561 taacaatcta tcaaattgaa atttttgtga acctaatgtt ataaatatca tttttttctc
     1621 ctattattta atatatccca cccaaataaa tagcattagg ataaaattgt agcatagttt
     1681 cccactgaac ataaaattga tctgcaaaat gatacatcaa ttttcctgtc tgagtagggc
     1741 tggttacttt tgcaaacgat tcaatataaa tcagtttttt tcccatcatt tttgttatta
     1801 agcagatagg gatcatagct aaaacacctg tacaaatcac gagatccggt ttttccttta
     1861 tatatataat aattgatttg aataaattaa taagtatagt acacaaaaaa aacttttctt
     1921 ttctatttac ttgtctcatg tagtacgttt tttcgccttt tgactcagct gaatattctg
     1981 tctgttctgt tataacaaaa ctctcatact tctccatcag tggttttaac atcattaact
     2041 gttcataatg ccccccagag gaagccgcaa aacatatctt tttgttcata ttaacctctc
     2101 tatcagtcac gctgaaaaat atctctagtg taaacttttt ctttaatatc atccaagcag
     2161 gtatcatatc gattggcaat aatagaatca cacatcgcct taaactgctc taaatcgttg
     2221 ataaccttac ttccgaaaaa agtctctcca tctttcagtg caggctcata aatcaccaca
     2281 gtcgcacctt tagcttttac gcgcttcata actccctgga ttgaactctg acgaaaatta
     2341 tcactgttgc tcttcatggt cagccggtat ataccgatta aaactggatt ttccctgttg
     2401 acatcatact gattatattc agcgtagttg taatagcccg ccatctgaag aacacgatct
     2461 gcgatgaaat ccttacgggt acgattagat tcaacgatag cctcaatcaa atttccagga
     2521 acatctgcat agtttgccaa caactgcttg gtatcttttg gcaagcaata acctccatat
     2581 ccaaaggatg gattgttgta gtgagttcca atacgaggat cgagacaaac accattgatt
     2641 atctgctgag tattcagccc cttcatttca gcataagtgt ctaattcatt gaaatagctg
     2701 actcgcagtg ctaaataggt atttgcaaac agctttactg cttcggcttc agtgaatccc
     2761 ataaaaagag tatccacgtt ctccttaatt gcacttttct gcagaagtgt tgcaaaaaca
     2821 tgagcagcat ttaacaagcg ctcatcagtc atatcagtac caacaataat acgagatgga
     2881 tacagattat catataaagc cttgctctcg cgcagaaact ctggactaaa aataatgttc
     2941 ttactccccg ttgtctcacg gactgattca gtgtagccaa caggaattgt agacttgatg
     3001 accatgatgg tatcaggatt cacgtccatc acaacctcaa taactgactc aacagcagaa
     3061 gtatcgaaat gctgagtacg actgtcatag ttagtcggtg tagcaataac aacgaaatct
     3121 gcggatttat aagcgctcct tgcatccaaa gtagcagtta agtcaagttc tttttctgta
     3181 agatactttt caatatattc atcctgaatt ggagacttcc gtttattaat cagttcaatc
     3241 ttttcaggga taatgtctac cgcggttacc tggtgatgct gcgccaatag tgtggcgata
     3301 ctcaaaccaa cataaccggt gccggctact gcgatgttgt actttttctt cacaatatct
     3361 cctctttcct ctcttaagta tggagaatgt tactcctacg gctattcatc atgtcaaaat
     3421 aacacagctt agaatgttga taaaataatt ttccagaatg tgataaaatc tctttcattt
     3481 ataaaccaca catctaattt tatctattct ccacaagaca tgtagagtat tctttaataa
     3541 caagatacga ccttactaaa tacttcaaaa aattatcata tttaattcat aaatataaat
     3601 cttcatttta aatgtatgcc caaaaattta tctgtaatgc gtcatcaaaa tctagattcc
     3661 aaatctttat caatttttat ccactcattc taccacttta gtaatatttt ctatttataa
     3721 cactttcgtc ctacattgat ccccccctct gaagtacagt ttttactgtc ttaaataaaa
     3781 ttttaaaatc taaccccata ctccaatttt ttatatattc cgtgtcaagt tttgctactt
     3841 cttcaaaatc agtaatatca cttctaccac ttacctgcca cattccagta attcctggct
     3901 taatagcaat tcttgcacga tgatgataat catattcttc atactctgat ttaaggcatg
     3961 gtcgaaatcc tataaggctc atctttcctg ttagaacgtt gaaaaattga gggaattcat
     4021 ctaaagacgt ggatctaata aattgaccga ttcctgtctt ttttgttcca tctggaagta
     4081 ttttatttcc gataattctt ggatcaaact ccatcttaaa cataagatta ttgctcatct
     4141 tattttcttt cattaattct acttttcttt cttctgcatc catatacata cttctaaact
     4201 tatacatctt gaacggttta ccattctgac ctatacgttc ttgtgcaaag aaaattgggc
     4261 ctggtgaatt aatataaata agtggagcaa taaagataaa aataattccc gttgcaatac
     4321 atccaacaat acctgcaaga atatcaattg ttcgttttat aaatgcctgt tgagttgtca
     4381 taacattgat acttgttgta agtactgtat attctcctat acgaccaata gattgttttc
     4441 ccaaagcact atctgatact tttgcaagat tcaaatgtac agtaaggccc atttcagagc
     4501 atcgttcaat aaattctttc atacacgaag tacttttatt caaaataata aagacttcat
     4561 ctacccatcc ctgacataag taatccgctg catttttgga atcagaaaca actgtcactc
     4621 cctcaacatc ttcaccaatc atgcttttat ctatgataat gacaccattt attttataca
     4681 ttttatagtt atgctctttc acattacgaa taactacttc agcaatacta ctagatgtaa
     4741 ctataataag tgatttgttc ccctcatttt tcattttatg tttcaagtaa tgtttccata
     4801 agaggcgagt tatatatgta attacaacgt ataaacctcc catcaagaaa agaacgactc
     4861 tagaataatc atttccaccc tgaacactaa tgagatacaa acttcctaac agcaccaaca
     4921 taatcgcttg ctttacagaa gaagcaaatt ctatatagta ccctcttttt aatacacctc
     4981 tatatgcttc aaacatgaaa ataactaaaa gatctgccag ttcaatgaag actgccatgt
     5041 tttgatacat tggaatcaca taaggattcc aatctacctg gcgtaagaca tacgatagat
     5101 aaaaagcaag ctggatacac aataaatcta aaataataaa atcaatatgt ttaacccatc
     5161 cccttgaact tttcttgtac attccttttt actcccctct ttttaataaa ataaattgta
     5221 ttacttgttt tctctcctaa atatctttct gaaggattat tgactcgaca aattggcttg
     5281 tggatattct tatatataga acatccgtaa gccaacttaa aactccttaa caaaattttt
     5341 ttactcatat tgtagatcaa aacaagtatc caagaaatca tattttattt cttttttctg
     5401 cttactcaac aacagctaaa agaaaaatca tcataaacaa attcaactaa gccaacgatc
     5461 cccttgtgaa atcaagtttt ttcttgattt acgaataaag agctaaaata aaccaaactt
     5521 cttctttttt acctcttcat agactggata actgatttca tccccgttga tcagatcctt
     5581 tgcgacttgt ttcatctgta ctactttttc cttaccaaag tcctttgcaa tctgctcata
     5641 gcactctttt aagtaaaagg tgcgcttaga gaccccatgg gcatcagaag cagccatctg
     5701 cacgagatta tgtttgacca tcgttttggc tgttttctgg atgtctttgc caaatacccc
     5761 aacataactt ggcgcagtga gttgggccaa acatcccata tctaggaacg gaagcaaatg
     5821 atttgggtct tcgcgaaaat gcacgttgcg ttctggatgg acgattactg gtgtctttcc
     5881 tagttctaac aactcaaaaa aaagccgttc tgcatataac ggcacttcca gtgtcggaaa
     5941 ttcgatcaga agatatgtat catctaaatc tgtaaataat atttcatctc ggtggatgtc
     6001 ttcgatgagc gttccggtaa tacgtacttc ttgtccttcc aataacgtca aaggcaactg
     6061 tcttttctct aattcggctt gtaacgaagc gactagagaa atgacttgtg atttttcatt
     6121 gctgtatttt ccattattgt ggtgtggtgt gcataaaata tgcgtgatgc cttgctgaat
     6181 cgctttttca gccattgcaa tacttgcttc taagttttct gccccgtcat cgattcctgg
     6241 taggatatgg caatgtagat cgatcatttc tcttgtctcc tctcttctta ttccttaatt
     6301 tccgtagtaa taaccagcat ctttgctatg ttctgctccg ttgtacacaa cacctaagac
     6361 tcgtgcttgt accatctcca gcagttcttt tgctttagtc aaggattctt tacgtgacat
     6421 attttcacgt acgaccaata aagtgccatc cgtttttaaa gacatgatct gtgcatctgt
     6481 tacggctact actggtggca tatcgaatat caccacgtca aataactgtc ttgcttcgga
     6541 taggatctga tccattcttg gcgagcttaa caattctgat gggttgggtg gcttaggtcc
     6601 actaggcaaa atcgacaagt tctcgactgg cgttcgttgg atcacatctg ctacacttcc
     6661 tgatgaacta agtgctgtac ttaatccact ggcattgttc agctggaatg tcttatagac
     6721 gacgggttta cgtaaatctg catcgactag tagaactcgt tgacctgatt tagcaaaaac
     6781 gactgcaatg tttgctgcag tggtggattt cccttctcca ggacctgatg aagtcacgac
     6841 aatcgttttg atttgctgac cagctgcgga ggcaaactgg atattcgtcc ggatcgtacg
     6901 atattgttcg gcgattggtg aagatgggtt tgttaatgtg atcaaactga ctgcatgagt
     6961 ttgtgctgct ttttgtttct gtgttcgtgc catttttctt cctcctagac tcttgagcgg
     7021 cttctgcgtg atggggctgt tttatcgttt gtagcttttg gtcgtgcagg agctgttcct
     7081 tttttacgga taccagcgtt caattctttc gctgacattt ctggaactgt tcctaagatt
     7141 gggaatccta atgtatctgt cacatatttt tcatctttca ctgtccgatc gagtaattcc
     7201 aataagaatg ccagacctac acctaccatc attcctaaca ttaagccagt cattaaattc
     7261 aatttattat ttggcgaaac aggtgtcgta ttggcaactg cacctgaaat gatcgagatt
     7321 ttatcaacat tcatcacatc ttttgcgttc tcttggaaaa cttgtgaagt tgtgttagcg
     7381 atctgttgag cagccactgc attcgtactt attgcctgaa tcgagaacat ttgtgaattt
     7441 tctgcctgat taactgagat ggcatctttg atttgttcag cagtcatttt cacattgtct
     7501 tctgtttcta aacgatcttt gacttcattg agcactaaat cactgacgat catatcttta
     7561 tacgtattga tcatttgcag gttcgtattg acatcattga catttgcagt gtctgattgt
     7621 ggcagtgtca cgatcaattg tgcttgtgaa ctgtattttg gtgtcaagat gaaaaatgtt
     7681 acgattcctg agatggccaa gccagcaaac attgagatga tgattattac caagtgtttg
     7741 cgtaatgttt caaaaatttc tttcaagctg attgtctctt ccattttttc tccactttcc
     7801 tatttattct ctagattcat ttgatctttt aattcttttt tcacacgatc aagttcttga
     7861 tcactgactt tttgatagga gatattatct tgcatgaagc cttctccttg cagctgatct
     7921 tgcttaatcg taccaaatgc ctcccgataa tctaaagcaa tttttttcat ttcatcaaag
     7981 ttcatgtctg ttttcatatt cgactctaga gcattcaaaa cggattgata gttgctgaca
     8041 ccgtttagac tcaatacttt tttcgctacg ccttctacga tctttctttg gcgcccttgt
     8101 ctaccgtaat caccatttgg gtcttcataa cgcatacgag aataagataa tgcctgatcc
     8161 ccgttcaatg taattttacc gattggaaag tcatagccat cttgtgagaa agtgaggtca
     8221 ttattgactt cgataccacc aactgcatca acgagttcct ttagtccttt catattgata
     8281 gagacatagt gatcgattgg gatgtctaca tatttttgta cggtatccat cgccatggca
     8341 gctccgccga atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc tacgatgtcg
     8401 acatacgtat ctcgaggaat gctgacgatc gttgtctgtt tgtcttcagg actgactgtg
     8461 gcaaccatca tcgtatctga tcttccttga tcgacccgac ctaaatctcc agtatcaatc
     8521 cccatcaata gaacagagaa agattcttgt ttgtccagat caactggttt cgttcgttct
     8581 ccttgggttc gttccactga ttcgtaagtt ttctcaatag aatgtgagag atccagatat
     8641 aattttgctc ctactcctac aattgctaat actaatacga taagaactgc taaaataatc
     8701 aaaaatattt ttttccctct tgattttctt cttcgtcttg ccatagtttc ctccgtttgc
     8761 taaccttgct tgaatcaacg ttttaactgt caatagctta tattgctata gcccatttgc
     8821 tgagcaacag tttgtgcttt tgttgcgtat gcggctctta ttttttcatt ccggatctgc
     8881 tctatttgac tgagcgccgt caaatattga tcgtaagtag cggtagctgt tacttgaccg
     8941 tctttcagca tcgtatcgaa tagctgatct agttgcgtga cttgtgcaat ttgggtatca
     9001 gcgaatccta gatattgcat aacgactgtt ttccaagcac tatccttaaa aaagctcata
     9061 ttttcacgta catgggcgac atctgcactt gccaactttt ctttgatgat gacatcatcc
     9121 acagcttgct gccagtttga tacgttttta gtaaataatt tattgacagc cgtctggatc
     9181 ttcagttttg attctgcatc ctctaactgc gttaagactg ctttcttttc ttcttggatc
     9241 gcagcagctt ctttaggtaa ttctgattcc ttgatgtcat aatcatctgc cgaaagtttg
     9301 atagaagata gtttatgtga caaatttgaa actgtttctt ctgttatttc aggatttagg
     9361 aaaatttttt cttttgtact gtacagctta tctacttctt ttgataaaga ggtcacggtg
     9421 tctttttgat tggtgatggc agattctgcg taattgatcc gttgatgata ttgccgctga
     9481 ttattgagat ggtatccaaa caagcaaatc acaatagcgc ctgtgcctac cgccacagta
     9541 gccaataact tgacgaacgg cttttccata gattcactcc taaatattga tgatagataa
     9601 gtaaagatgt gattcattca catcagaatg agcgcttttt attttaaaag cataattaaa
     9661 ctaatatatt catagaaa
//
LOCUS       DACCIQ010000094         9650 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCIQ010000094 DACCIQ010000000
VERSION     DACCIQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.096"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            185..1183
                     /locus_tag="IVM62_002333"
     CDS             185..1183
                     /locus_tag="IVM62_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6846258.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVM62_002334"
     CDS             1295..1804
                     /locus_tag="IVM62_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP6846259.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVM62_002335"
     CDS             complement(1834..2493)
                     /locus_tag="IVM62_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP6846260.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVM62_002336"
     CDS             complement(2495..3259)
                     /locus_tag="IVM62_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP6846261.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVM62_002337"
     CDS             complement(3267..3944)
                     /locus_tag="IVM62_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP6846262.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVM62_002338"
     CDS             complement(3981..5366)
                     /locus_tag="IVM62_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6846263.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVM62_002339"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVM62_002339"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVM62_002340"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVM62_002340"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP6846264.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVM62_002341"
     CDS             6305..7162
                     /locus_tag="IVM62_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP6846265.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVM62_002342"
     CDS             complement(7243..7578)
                     /locus_tag="IVM62_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846266.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVM62_002343"
     CDS             complement(7645..9015)
                     /locus_tag="IVM62_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP6846267.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVM62_002344"
     CDS             complement(9093..9629)
                     /locus_tag="IVM62_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6846268.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2824 a         1993 c         1619 g         3214 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca
//
LOCUS       DACCIQ010000095         9629 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCIQ010000095 DACCIQ010000000
VERSION     DACCIQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.097"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            168..362
                     /locus_tag="IVM62_002345"
     CDS             168..362
                     /locus_tag="IVM62_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846269.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVM62_002346"
     CDS             631..930
                     /locus_tag="IVM62_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846270.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVM62_002347"
     CDS             complement(1027..1461)
                     /locus_tag="IVM62_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6846271.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVM62_002348"
     CDS             complement(1693..2403)
                     /locus_tag="IVM62_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6846272.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVM62_002349"
     CDS             complement(2393..3253)
                     /locus_tag="IVM62_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP6846273.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVM62_002350"
     CDS             complement(3258..3896)
                     /locus_tag="IVM62_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP6846274.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVM62_002351"
     CDS             complement(3911..4210)
                     /locus_tag="IVM62_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6846275.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVM62_002352"
     CDS             complement(4253..5737)
                     /locus_tag="IVM62_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6846276.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVM62_002353"
     CDS             complement(5757..6218)
                     /locus_tag="IVM62_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6846277.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVM62_002354"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVM62_002354"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP6846278.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVM62_002355"
     CDS             7521..8291
                     /locus_tag="IVM62_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6846279.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVM62_002356"
     CDS             complement(8564..9391)
                     /locus_tag="IVM62_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6846280.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3010 a         1955 c         1504 g         3160 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaa
//
LOCUS       DACCIQ010000096         9574 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCIQ010000096 DACCIQ010000000
VERSION     DACCIQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.098"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            134..871
                     /locus_tag="IVM62_002357"
     CDS             134..871
                     /locus_tag="IVM62_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846281.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(888..1304)
                     /locus_tag="IVM62_002358"
     CDS             complement(888..1304)
                     /locus_tag="IVM62_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846282.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            1505..1699
                     /locus_tag="IVM62_002359"
     CDS             1505..1699
                     /locus_tag="IVM62_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846283.1"
                     /translation="MYTAREASLILGKGRNYVTNIINYYPNRLPKGSFKKVGDKTYII
                     TEEGIEALRSQTENKNKKKE"
     gene            1696..2943
                     /locus_tag="IVM62_002360"
     CDS             1696..2943
                     /locus_tag="IVM62_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846284.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3211..5601
                     /locus_tag="IVM62_002361"
     CDS             3211..5601
                     /locus_tag="IVM62_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP6846285.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            5650..7485
                     /locus_tag="IVM62_002362"
     CDS             5650..7485
                     /locus_tag="IVM62_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6846286.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            7560..8417
                     /locus_tag="IVM62_002363"
     CDS             7560..8417
                     /locus_tag="IVM62_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6846287.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            8407..9516
                     /locus_tag="IVM62_002364"
     CDS             8407..9516
                     /locus_tag="IVM62_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6846288.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         3536 a         1463 c         1908 g         2667 t
ORIGIN      
        1 tggtctagat gataattgta atatagaatt gtagacttaa ataaagcata aaataccttt
       61 tatgataagg tatagctctt aattttaagc agttttagat ataagattga aagtaaagtg
      121 ggggagaata ataatggcag atcataaatt agaaaagtta ttagaagaaa cgtggaatcc
      181 aaaagaattt agtgaatttt ttatggagaa ttttgaaact gacttagcag tgattgttaa
      241 agatgctctt agagagcaag ggtatcctga aactgcaaac tatataaaca ttaattttac
      301 tttgtatact gaaaataaag gaacttggga tttttgggct actttagcaa ataaggagtt
      361 aagtgataaa tcagacacag gaattcgtaa cttttttgaa tcaaacagag atgattatat
      421 gtatgctaac aatcaaaata aattgaactt tagagtagag tttgatgaaa cacctgaaga
      481 atttatagaa agacaaccgc ctaaagaaaa tgtagcaaag gtattggaag atcgttggaa
      541 ttcagatgaa atagtttcaa caattagcga actaggcgga caatatgaac cgctagtaga
      601 agcggttaga gaagagctaa gactgaataa gtttcctgat gttcaaaata ttgatgtttc
      661 tcaaattgaa ataaacgtca agatcacgaa taaacttgat tatggtagtt gggcagatat
      721 agcattagaa aaatacatct actcaacgtt aaaagagttt atcgaaaata gaatggatat
      781 catgtatcta cagcatccac agtatttaaa ctttggtgtt gaaatagcta cacctcttga
      841 agaatggaaa atggagcaag ggcttgacta gcctttgctc cattttttta ttttacaaat
      901 gaatacagtt cgtcgttttt accaagaatg attgtttgcc caactttgag attgtcaggt
      961 gatccttcag cggtttgtaa ggagaacgtt ccattgaact tatcggatac cgttataggt
     1021 agctttaatt ctgatccttc ctcatctttg agaattgctg tttttcgttc atcaaaataa
     1081 acagtcttta ttaaataatt tttcgtagtg atctcagcag atttgtcttt ttttgtagta
     1141 gtaccagttt ctttagaatt agaggtattt tctacgacta ttccatcttt ggcatttgag
     1201 cttgtgttta tttgattgaa tattttcagc gacaagataa cgctaagaat agtcaatata
     1261 gaaacaagtg taattaatac tgataatttc ctttttttgt tcattttttc ttaacatcct
     1321 tttttgtttt gattagttag atattcctta tctaacaagc ctttttaggc tattaaatga
     1381 agataaaccc caaaaaatat tgcaattatg acagtgcaat ggtactatta ttatacaata
     1441 aattttgcga aaggggtagg tctaaatcgc aaacaaaaag aaattgaacc tagacaaata
     1501 caacttgtat accgcaaggg aagcaagttt gattttgggt aaaggtagaa attatgttac
     1561 aaacataatc aactattatc caaatcgttt gcctaaaggt tcgttcaaga aagtgggtga
     1621 caaaacctat attataacgg aagaaggaat agaagcattg cgatcacaaa cagaaaataa
     1681 aaacaaaaag aaggaatgaa tatgaagaga aattcgcttg gtatggtttt attggcatca
     1741 gctgtacttg cggggttatc agtaccagta acctcacatg ctgatgaatg gactgcaaac
     1801 acaccggatc aaatccaaat tacaacagga caatcaagtt atgcgttcaa aaaaggagat
     1861 actatttggt caactgtaga gaagcttaaa agcttaggtt acaagactac tgttgctgag
     1921 tttgccgaat tgaacaatgt tgatttggca aaaggagaac aacggactat cccgattgga
     1981 cgaactttca acatcagtaa agatacatta acagttaagg aagcagatgg cgaagtagtt
     2041 agtcaaactg ttcaaaaaga taaacctgat cctattgaaa atacggagca agagaacaaa
     2101 aaatccgaga ctacaaaaga atcggaacca acagcaaaca cttctgataa gacagaaaca
     2161 agtgcttcaa gtgttgatgg agcaagttca tctaatacaa ataattctac aagtgagaaa
     2221 gaagcatcac agggaacaag tgaaagtagc actacggaag ctgatactgg aacatcaaca
     2281 aataactcac aatcaacatc aaatgatgca aatgaatcaa ccagtacatc ttctagtgat
     2341 caatcacaaa caggttcgag tgatgaaact gacagttcta cttcaccaag taaacccgaa
     2401 caaccaacta atgttgtgct aggcgttcca tttattagtc aaggtaacac aatgctttgc
     2461 gaaggaacat ctttgctaga agcgttacat tataaaggtg ttgcaacaaa tcaagattta
     2521 atgacttttg ttaaatcaat gccactatct cctgacaata atccaaataa tggtttttct
     2581 ggtgaatgga gacacaatgt ggatggaact tatcaaggta tgaatccggc tcctgtagtt
     2641 tcatggggtg ttgctaatgg cggaaatgtt gtagatattt ctggttcatc ggtacaagga
     2701 ttaaaagatg agattgtaaa tggaaatcct gtagtagcgt gggtaactta tgcctttgaa
     2761 cctgctcaac ataaaccaat gttttggggc gatgctattt ggaatcgaca tgtagtagcc
     2821 gttgatggat ttaaagacgg tttttaccat gttgtcgatc ctgtgtttgg ggcaagttgg
     2881 attaatgcag gatcatttga aacagcatat aacgtatctc aattagcggt ttcggtacgt
     2941 taataaaaga tatcgtttat atactaagta gttatatagt ctttatttgt ccttgcttaa
     3001 ctacttagaa aacttgttgc tcatggtggg tagaactaaa aggggtgatg ctatgaatac
     3061 tagagcgttg aaacagcttg ctttcaacga aaaaaatgat taataggtgt ggtgatttta
     3121 acttaggaat gggcgcgaag gtcattacca caccgacata tcgtggctca ttttcttcgc
     3181 ctaactggta taaagaagga gtaacgagag atgaagcatg aaaaaacgat aattttaaaa
     3241 tcaaaaataa tttattgttt tgcgacttta gtaatgatgt tatctacgct tataatgcct
     3301 ttttcagcaa taagagctga agcagatgtc acaaaaccga caagtgcaaa atttatagaa
     3361 actggcgtaa aaacagacgg ttatattagg gttaatatgc cgaatcatcc aaatgaatgg
     3421 atgatttcaa gtcaatttaa agattctcat ggaaatattg gatattgtat ggattcagaa
     3481 ttgccatccc caacaggaag tggagccgga agcttgaagt acaagggagc tggcagtgat
     3541 gagttctatc gtatgtttaa aggtggtttt ccatcaaaaa cagcaaagga actgggtgca
     3601 ggaaatgata cagaagcatg gtatgcaaca caattagttt catgggtttt agccggaaac
     3661 tttaaagttt cacagattgt atggtcacat ccaaaccata ccgccgcaga aacagctcgt
     3721 gtaaaaaaag catttgaaaa aatttatgac tatgctaaga atggaaaaga tacgcccaac
     3781 actgagtttt ctatcacagc ttctaagaca gctgacgaag gaaaatatca cactttcaca
     3841 tataaaacag catcaaataa gactggtaat gcgaagctca cttttacatc agcaaaacca
     3901 gctggtatga agatttatga tgcagatggt aaagaaatta ctaacaacac tgtgaaatta
     3961 aattcaagtt ttactattaa agtacctgtg actacaccaa gtggtacttt gtcatttaaa
     4021 ggtactgcta atgttagcac aaccaatcct tttacttttg atggacgagg tgtgtatcaa
     4081 gatgccgtag ttatgatcac tacttcagaa acaaaggact ctaaatcttt gagtgctaaa
     4141 tggacaagag ctgttggtca ggtaacagtt aagaaagttg atcaagatgg aaatgcttta
     4201 aaaggagcgg agtttactct aactgacaat aacaatgcta aatctgttaa gacaaccgga
     4261 gatgatgggt tagctacgtt tgatttgcaa caaggcttaa catacagctt agaagaaaca
     4321 aaagttcctg aaggttatca aggttcattt aaaaaggatg gtattactgt taataccgat
     4381 ggacaagaat ttagttacac tgctacgaac acaattaaaa aaggtaaagt aaaagttaaa
     4441 aaggttgacc aagatggaaa accattagct aatgctgaat ttaccttgac ggatgattca
     4501 tcccaaacac aagcagaaaa aacagatgaa aacggacttg caagcttcga tattgtagca
     4561 aacaagacct acagccttga agagaccgga gtaccaactg gttatacagg cgtattcaag
     4621 caagaaggaa ttaccgttaa agatgatgga caaacttttg aatatacagc tacaaacact
     4681 ttgaaaaaag gtttagtaaa agttcataaa gtcgatcaag atggaaaacc tttggcaaat
     4741 gcagagttca ctttaaccga tgaaaaaggt gcaacacaag tagaaaaaac agatgaaaat
     4801 ggacttgcaa gcttcaatat tgtagcaaac aagacgtata gcctagaaga aaccggagta
     4861 ccagtaggtt acacaggtgc atttaagcaa gagggtatca ccgttaaaga cgatgggcaa
     4921 acttttgaat atacagctac aaataatttg aaaaaaggtg tagtaaaagt tcataaagtc
     4981 gatcaagatg gaaaaccttt agcagatgca gaattcacct taacggatga aaaaggtgta
     5041 aaagaagtta aaaagacgga tgcaactggt ctggctacgt tcaacattat tgcgaacaaa
     5101 acttataact tagaagaaac aaaagtacca acaggttaca caggcgcatt caaacaagaa
     5161 ggaattaccg tcaaagatga tggacaagtc tttgaatata ctgctaaaaa cactttgaaa
     5221 aaaggtgtga tcaaagttca taaagtggat gatcaaggga aagcattagc aaacgcagaa
     5281 ttcactttga cagacgacaa aggtgtaaaa gttgtacaaa aaacggacag caatggcttg
     5341 gcaacattta atattgttgc caacaaaacg tacaaaatcg aagaaacaca aaaccctaat
     5401 ggttatacag ggaaatggtc taaagaaggc atcaccgttg ctgacgatgg acaaaccttt
     5461 gaatatacag taattaatca ccttgtacct gaaacagtaa gaacaggcgg aatcggtaca
     5521 tcagccttcc ttcttgtatt tctaattgca ggattgagta tggtgctact gtattatcga
     5581 gaacagcaca aagcaaaata aaaaagtgtt aatggaaaag aagtaaatat gaaaaaagaa
     5641 ggctgacgta tgaaatcaaa aaaaattttt tcaaatttaa tgctatttgg tactttgcta
     5701 ggagtgggat tgggcgcaag ctcaatctct tctagccttg gactaggggg gcaagtagca
     5761 tatgcacaac aaaactataa ttatcaagga actggaacga ttcatatcca taaagtaaca
     5821 aatactaatg gggatgggaa ccctgtctca gatattgata acaaagggaa agaaatagct
     5881 ttaccaaatg gtgtaaaacc tcttgaaggc gttgagttct cttatgtgaa acttactaaa
     5941 gatgaagcta aatccttggg atggacggat agtgatacta caaaaggtca acatattgat
     6001 gatgctaaag cacgattatt actaaacaaa aaatacagtg aagtaagtag aatgaagact
     6061 gattcacaag ggaatataga catacctata caagtaaata cagacgattt aacagataac
     6121 ttgtatttga ttgttgaaac aggaacagtc aaaggtgtag ttgaagaagc gtatcctatg
     6181 attgttaacg tacctgaact aaacggatca gatgatcaat actttaatta tgatgtacat
     6241 gtctatccta aaaactattt ccaattaaac tcagcagagc ttgataaaaa agacgaagat
     6301 ggaaaggctc ttgccggaac tgtttttgaa ttgcgaaaag atgatggatc tgatcaggtt
     6361 gttggaactt ataaaacaga cagcacaggt aaattaactg tagatggttt ggaattaggt
     6421 gactattatt tcctagaaac cgatttggga acagatgcta ataaaacaac ctatttacta
     6481 gatggtaaaa aattcaaatt tagcgttaaa cgtggggaga ctgctaaagt agttcttgat
     6541 gttaccaact acaaacgacc tgtaattact aaggtaattg ataaagaaac cgttgatact
     6601 ggcgaagatg ttcaatatac attgaaacca tcaattcctg gtaatattgg taattacgaa
     6661 aaatatgttg tcaaagatgt tctaggtgca gagttaactt atgattcatc agaagtgatc
     6721 gaaggaatga ctttagatac tgattacagt atcgaacgtg acggacaaac gacaaactat
     6781 gtctttactg aatcaggtat ggataagctt cagaaacaat ttgatgcagg ggatcgcttc
     6841 tttacaattt tgatgaacgc tcatactaat agtaaagcta aaatggatac accacttaag
     6901 aatgatgttt caacgatcta caacaatgga tatgaacccg aagacgaaac accagttgat
     6961 cacgcttata caatcattgg tgatcaccaa ttcattaaaa cagatggaga tacaagcaaa
     7021 gcattgagtg gagcaaaatt tgtagtgaag aacaaaattg atccgaataa agttacttac
     7081 ctaaaacaag atccaacaac gaaatctgtt acttggattg cagataaatc taaagctact
     7141 gaattcgttt ccggatcaga tggtaagttc caaattgaag gcttgcaata tacctatgat
     7201 gtagattttg ataaacaagg taatccaaca atcacagata aaaaaatcaa tgattatgct
     7261 cttgaagaag ttgttgctcc ttcaggatat acaccaacta gaaatgatgt tccatttgtg
     7321 gtatcgaatg atgatgcttc tagcgaagta acagacatca agaaccttaa aacacctgaa
     7381 acagttcgta caggtggaat tggaacagta gtatttctag tggttgcagg tgtggcagga
     7441 acagggctat atgttgtcta caaacgagaa caacacaaag cttaatgaaa taaaattccc
     7501 tagagtgttt ttggctctag ggtttctttt taaaaagtgt gtgaaaagta ggtatgagca
     7561 tgaaaaagaa gaatatcaag ttgatcatct taaaaattgg gatgttgcta tgtttaggag
     7621 taggtctata tcctgtaatt actcaggctt atacctacta ccaaactcaa caaacactaa
     7681 actcctataa ggaaaaatat gatgaagctt ctgaaaagga gaatgaagaa gtcttcaaga
     7741 gatattcaga agttaatagg aaacgaaaag acacagaaca aacctcagtc acgattcctg
     7801 aagataataa caatattccg tcaaatggaa atcttcagaa ggataaatta ggtgaattag
     7861 tgggtgaaat tcttatacca aagattaatg tggatttgcc aatttatgat ggtgtaaacg
     7921 atttccaact atcagcagga gcaggaatat taaccggaac tgattatcca actggtggga
     7981 ttggaaacca ttctgttata tcagctcatt caggcattcc taataaaaaa tattttacag
     8041 atttagagtt gctcaaactt ggtgataaat tttttattga tatttcaggg aaaaaacttg
     8101 cttatcaagt agatcggata aaaacaattc taccaaccga atttgaggat ttgcttcctg
     8161 tggcaggaaa agattatgta acacttatga catgtacacc tgttggagat aattctcata
     8221 ggttactcgt tagaggggtt agcatacctt accaaaaaag tattgttgag aaagaaaaga
     8281 agaaagaaga tttaaaggta aaggcgcaga tgaactggta tcctatcgta gttgtagggg
     8341 ttttattaat aatagctgtg atagtcatta ttaaaaaaat agtagcaaag agacgaggaa
     8401 tgaaaaatga agaataaaat actacttttt ttgactctta tttgcacttt ggttttaaca
     8461 atttatcctg caaatcagct ctatcatcaa ttcaaaacaa aagagaatgt ggcggaaatc
     8521 caagagaaaa ttgagaaaaa acaaactcct tcaaaagagg aagtggcaaa aaaagtcgaa
     8581 caaataaaat cgaacagcgg taaccaagga ttagacaata ctcagaaaac agataaagga
     8641 gtagacaata ctcaggaaac agacggtgga tcatttgata ttcgtgaagc tgaaggaaag
     8701 gtcaatccat acacaggaga tgttttttca aaaaatcaaa cgaaagattt tgaaccatct
     8761 acctattcag gagcggttgg ttatgtctat attccaaaaa taaatgtgct agagcttttg
     8821 tttctaggcg cttcacagga gcatttatat agaggtgtgg cacaaatgag tgctactagt
     8881 ctacctttat caggtaaagg tcatcaaaca gtaatagcag gtcatcgtgg cggttatacg
     8941 tcaccacaat ttctttatgt tgatcaatta gtagccggag accacattta tatagattat
     9001 cttggtgaaa aagcagttta tacggttttc cgaacagacg ttattagaaa ttacggtcag
     9061 aattcaacac tgactgatat tcctgattta gatatgttaa cactttatac ttgtcatcca
     9121 tatcctcaga actacgatcg tttattagtt agaagtctaa gagatgaaaa agcaaaatac
     9181 atcacgccac aagtaacagt tggcaatacc gcatacgctg atgccattga tagcgagtcc
     9241 gtttcagata agacagaaac tgttacatct tctaatgatt tatttgaaga tgttgatcgt
     9301 gccaacaagg aattagatgg aaataatgat cttaatattc cggaacaagg tagcgtagag
     9361 atgcaagaca gaacgtttgc tgaaaaggca agttcaatat ttacaggaat taccaataaa
     9421 catcagaaaa tgcttttagt catttggact gtaataggag cattagcaag tctaaaggtt
     9481 cttattcttt taatcaaaga gtttcgaaaa gcataatgaa gattataaaa attgatagag
     9541 tacagtaaaa agggattgat actattattg atct
//
LOCUS       DACCIQ010000097         9530 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCIQ010000097 DACCIQ010000000
VERSION     DACCIQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.099"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            229..1464
                     /locus_tag="IVM62_002365"
     CDS             229..1464
                     /locus_tag="IVM62_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6846289.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2463..2723)
                     /locus_tag="IVM62_002366"
     CDS             complement(2463..2723)
                     /locus_tag="IVM62_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6846290.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2750..4051)
                     /locus_tag="IVM62_002367"
     CDS             complement(2750..4051)
                     /locus_tag="IVM62_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6846291.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4343..4801)
                     /locus_tag="IVM62_002368"
     CDS             complement(4343..4801)
                     /locus_tag="IVM62_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6846292.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4814..6139)
                     /locus_tag="IVM62_002369"
     CDS             complement(4814..6139)
                     /locus_tag="IVM62_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP6846293.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6156..7568)
                     /locus_tag="IVM62_002370"
     CDS             complement(6156..7568)
                     /locus_tag="IVM62_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6846294.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7585..8349)
                     /locus_tag="IVM62_002371"
     CDS             complement(7585..8349)
                     /locus_tag="IVM62_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP6846295.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8453..9328)
                     /locus_tag="IVM62_002372"
     CDS             complement(8453..9328)
                     /locus_tag="IVM62_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846296.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3089 a         1983 c         1442 g         3016 t
ORIGIN      
        1 acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt
       61 ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt gtgaaaacag
      121 cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg ttttttagta
      181 tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat gaagacaatt
      241 ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga ggttgccaaa
      301 gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt tgctcctttg
      361 atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa aaaacaagaa
      421 gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac caaagaaatg
      481 gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc tcgactgatt
      541 gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat tcctttagta
      601 tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc gctgatgccc
      661 cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac aaaaaagcgt
      721 atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt
      781 cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat cacaacgcct
      841 aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg tccaattttt
      901 gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa
      961 gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc
     1021 aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc tattgactat
     1081 ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata tctgtctagt
     1141 catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg gaccgtccaa
     1201 actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga gcaaagctgt
     1261 aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc agtgactgaa
     1321 agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc
     1381 acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga aatcgaaacc
     1441 ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat agagactcat
     1501 cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa atagtggatt
     1561 gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc cttaaaataa
     1621 aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct tagtcaaatt
     1681 ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa gaccataata
     1741 attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc gctttgtaga
     1801 gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt accactttat
     1861 ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg atgcatactc
     1921 ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat gttctatgtc
     1981 gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc ccagcacaat
     2041 tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat taccattcat
     2101 acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat ttgacttgat
     2161 cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc cattttttcc
     2221 tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa agggctgtga
     2281 caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct tgcatctgcg
     2341 gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta agaagcaatt
     2401 ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca cttttagaac
     2461 atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt gatagcaaaa
     2521 attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg tacaatgatc
     2581 tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt gatttgaata
     2641 tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa gataaaaaca
     2701 aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact tagacagtct
     2761 ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca taagctttta
     2821 caaatggata atagataata aatgctatca atgtgttgat catgatcaag ataacaacac
     2881 gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc catgttggca
     2941 aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata atagtcaaaa
     3001 ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata ggagctccaa
     3061 aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga ccaacctctt
     3121 tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc gttgctcctg
     3181 caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag ggtaattggg
     3241 tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc agccacattg
     3301 gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac aacaaatgga
     3361 tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg gttaaaggct
     3421 cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga atcagccaag
     3481 taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca acagcaggtg
     3541 gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga tatacttcca
     3601 ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa gaactcatag
     3661 gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga acttgtaaca
     3721 taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca tatgatttag
     3781 ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc gtcatcctaa
     3841 agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca ggaaacggta
     3901 aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc
     3961 cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt gggacaagtt
     4021 tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa taaaatatga
     4081 accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat taagaaattt
     4141 tatttcaata tattctctta aataatcaaa tttattttta agaatcctga aaataagttt
     4201 tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc
     4261 tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct
     4321 atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc tttacttctt
     4381 ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg atcaccgtat
     4441 ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc ccttctttta
     4501 cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca actgtatcta
     4561 aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg gcatgtcggg
     4621 taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca tcatgattcg
     4681 ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt actgctgtta
     4741 atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta aagaaatgca
     4801 ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg atcatttcat
     4861 ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta aatgcttgta
     4921 aagctttttg ataagaacct tcaaagtaag catctactag taacttctct actgcaactt
     4981 gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta attggctcca
     5041 caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt agattcggaa
     5101 tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc aacaaggaaa
     5161 tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat acttcttcca
     5221 caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt ccatccatga
     5281 ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca ggatataagt
     5341 aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg actgacatca
     5401 tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact tgcatttctt
     5461 ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct ttatcataaa
     5521 tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa ttcttacgat
     5581 cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca caggcattaa
     5641 tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac tctggatttg
     5701 tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca atttccaata
     5761 aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt tcttgtccta
     5821 ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat cctccaacac
     5881 gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca tccgttgcac
     5941 gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc aagtacatat
     6001 ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc aaccgttttt
     6061 cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct ccagctatag
     6121 tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa tttattgaat
     6181 tctgccataa cataagtcac ttctaagccg atgataattt gataattttt ttcagttggt
     6241 ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat ggtgggatcc
     6301 actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt ggcttttcct
     6361 ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc taaccctaac
     6421 tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg tcctaatgtc
     6481 tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc aaaaaagaaa
     6541 attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat gagataatca
     6601 acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca gacaatccct
     6661 gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa catgaaacta
     6721 aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct tcccatgatt
     6781 cctttgacca tctctttatt ttctttataa gctgctttat agatagctag acatatagct
     6841 ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc ctgaggatct
     6901 ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga tgtatagctt
     6961 cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg gatcagcaaa
     7021 cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag cagttttgca
     7081 aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac agctgcgatt
     7141 gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata agaaaaaact
     7201 actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa accggaaata
     7261 attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc ttttttcaag
     7321 gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt tttgacaaaa
     7381 ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc atttccagct
     7441 gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat caatcccgct
     7501 aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac atttgattta
     7561 acattcattt ttattcctcc tctattaaat atcctcatat gttactaact caatcaaagg
     7621 atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt ggtgggtacg
     7681 aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg cgtgacacat
     7741 gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat attcttccac
     7801 actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga aacaaggctc
     7861 aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc
     7921 atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat gagtatgatg
     7981 atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa tctgagcatt
     8041 ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat aagataagga
     8101 tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt tgccacacgt
     8161 caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt tcacagcatg
     8221 ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc gctgataagc
     8281 atccataatc ccataattca caccatggct atagccaaaa tcatccgaat taaaaatcac
     8341 ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa ataatcatag
     8401 tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta ttttaataga
     8461 ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca agttgaaatt
     8521 gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta tgtttaaatg
     8581 tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc aagcgtttaa
     8641 ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta tcataaacgt
     8701 ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact atttttaagc
     8761 tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg atgaatccta
     8821 cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc acttctactg
     8881 ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt gctgtgacat
     8941 ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat gtaatataga
     9001 tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact tcttccgcaa
     9061 aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg atcttttctt
     9121 tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta ctcactttag
     9181 cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca tcgatcaata
     9241 cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct ctatatgata
     9301 aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa tttttaaaga
     9361 caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat agtagcaccc
     9421 tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat cttttttact
     9481 cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
//
LOCUS       DACCIQ010000098         9457 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCIQ010000098 DACCIQ010000000
VERSION     DACCIQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.100"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..639)
                     /gene="eda"
                     /locus_tag="IVM62_002373"
     CDS             complement(<1..639)
                     /gene="eda"
                     /locus_tag="IVM62_002373"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6846297.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIK"
     gene            complement(723..2150)
                     /gene="uxaC"
                     /locus_tag="IVM62_002374"
     CDS             complement(723..2150)
                     /gene="uxaC"
                     /locus_tag="IVM62_002374"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6846298.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2224..3303)
                     /gene="uxuA"
                     /locus_tag="IVM62_002375"
     CDS             complement(2224..3303)
                     /gene="uxuA"
                     /locus_tag="IVM62_002375"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6846299.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3306..4937)
                     /locus_tag="IVM62_002376"
     CDS             complement(3306..4937)
                     /locus_tag="IVM62_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6846300.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5254..5925
                     /locus_tag="IVM62_002377"
     CDS             5254..5925
                     /locus_tag="IVM62_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6846301.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(6761..7717)
                     /locus_tag="IVM62_002378"
     CDS             complement(6761..7717)
                     /locus_tag="IVM62_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846302.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(7826..8071)
                     /locus_tag="IVM62_002379"
                     /pseudo
     CDS             complement(7826..8071)
                     /locus_tag="IVM62_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8216..8638)
                     /locus_tag="IVM62_002380"
     CDS             complement(8216..8638)
                     /locus_tag="IVM62_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846303.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(8777..9207)
                     /locus_tag="IVM62_002381"
                     /pseudo
     CDS             complement(8777..9207)
                     /locus_tag="IVM62_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         2954 a         1939 c         1394 g         3170 t
ORIGIN      
        1 ctttatctca taaaacttat ctatatattt actagctatt cgagttactt cttcaaaatc
       61 acctttttca acaggagcca acaaattccc acctacacca actgcaacaa cgcctgcttc
      121 aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat ttacatatgg
      181 taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg gaaatagttt
      241 aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca tacaacctgg
      301 taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa aagatggact
      361 tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat ctaaaactgt
      421 accggctcca attattacat ctttttgatc agcatatttt ttagcaattt ctttgattat
      481 ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac ccgcagtaat
      541 tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg caatgactcc
      601 agccgcttct aacttactta aaatttctac ttttttcata tctatctcct tcttttatgg
      661 atacatactc tagctagttg tactaactta caataaataa atttaagctt ttattttttg
      721 tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt acgataacaa
      781 atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc tgctttaacc
      841 cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg atagcttaga
      901 aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg ttgcgccatc
      961 gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc acatcccaat
     1021 tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag tggaagccaa
     1081 tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt ttcagcagtg
     1141 tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga atcaccaccg
     1201 acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc atttaaatgc
     1261 atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg aatacctgta
     1321 acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc ttttattaac
     1381 agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa accgtgatct
     1441 gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc ctgcttcaac
     1501 ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat atagttcgaa
     1561 aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa taccttgaaa
     1621 gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg atcatccgtt
     1681 gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata ggaatccgtt
     1741 tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga aattgtcaaa
     1801 tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg attacctggc
     1861 gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc tgcaccactg
     1921 atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg atctccagct
     1981 cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc tagcaaacat
     2041 gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa tttatgaaaa
     2101 agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat atttaagcac
     2161 tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga ctccattata
     2221 taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat aaactgcacc
     2281 tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa tcatccgacc
     2341 atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta atatcttata
     2401 catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga gtccattatc
     2461 acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt gacaaaattc
     2521 agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag tacataacgt
     2581 aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt cacgattttt
     2641 aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa ctgccatttt
     2701 aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa agttctctcg
     2761 caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt gtgataaccg
     2821 atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt ttctaataat
     2881 ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag acccatcatc
     2941 taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat tataacagat
     3001 aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct cgatgtattg
     3061 atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct caataacttt
     3121 caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt ctctcggcca
     3181 gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg ctgggatctg
     3241 acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa aagtcatttc
     3301 catagttact ttcctccaac ttctgttata tatttattga ttgttttacg aactgcttga
     3361 ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc aacttgatat
     3421 aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca atgaatcttt
     3481 gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc taccaataaa
     3541 ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa ataacgtaac
     3601 caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc tttttcgaca
     3661 tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc tgttgcaatt
     3721 cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac ttcatcaata
     3781 aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc tacataacct
     3841 atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat tgatttatac
     3901 cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca tgtcgtaacc
     3961 ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc agccgcttcc
     4021 agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac tttttcagta
     4081 ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa gtcttcgaaa
     4141 cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat tctatcaatc
     4201 atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa tttttcagaa
     4261 acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg aaaacgttga
     4321 ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc agcattaaac
     4381 ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt aggtccagat
     4441 tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa actgtatcct
     4501 ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata tttaaccact
     4561 ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt tactgctaaa
     4621 agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc attgttgtag
     4681 tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac tactccggat
     4741 tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc tcgataaaga
     4801 tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt gaccgcttcg
     4861 acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga atcttgccag
     4921 tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg ttacaattgt
     4981 agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt cttttttcta
     5041 ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata ttttttagaa
     5101 ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt tgttttttta
     5161 atagctagta tgttactttg aaatttgata ttatataacg agcatagaac taaaatatca
     5221 aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag tctccaaaat
     5281 caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc tggaaaaaaa
     5341 gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat acgtgaagca
     5401 ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg aaattatatc
     5461 tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca tttagaacga
     5521 caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt tttagaaact
     5581 ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt ttttcataat
     5641 gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt atggcaatgg
     5701 ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa aacggaagga
     5761 ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt gaccatgcaa
     5821 gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct tgatgaacaa
     5881 aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct aaatttcttt
     5941 tttttaattt atttacattt ctgattacta cagaaaacta actacttttt ttatgtaaag
     6001 ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca tgtgaggtcg
     6061 taaacaaaga ttgaactatc tacattcata ctctataatc tgtttatttg cagactgata
     6121 atagcaaaag aaaacttata atcaaataat atcaacattt gatcacataa tttgagattg
     6181 aatgctgatt tacctatgtt ttacgtaatg gtctttagtt atttttgctc ataaaatgca
     6241 taaaacacgc gtttttatgt gtgcttgaaa tttcttggct ctataatttc taagactgat
     6301 aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc cattagattg
     6361 aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc tataaaacta
     6421 cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata tccgaagtac
     6481 cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt cttatatcgc
     6541 tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta cgagtacttt
     6601 ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa tagtttcttt
     6661 cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc aacttaattc
     6721 tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa atgttttttt
     6781 gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc actagctttt
     6841 ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac gattgattta
     6901 attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct ggtaccgtca
     6961 gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct aaaagctccc
     7021 tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga tagcatctaa
     7081 attcctgctg atcagccgct acgattactt ttctatcact aacttcacca aggcgctgca
     7141 acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt tcccatagct
     7201 tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata gctgaccaga
     7261 agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt ttttgcttta
     7321 cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca gctaaagcag
     7381 tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc gactgagtat
     7441 aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg ccaccactct
     7501 caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc cgtagcattc
     7561 ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct aaatgccaaa
     7621 tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt tgttgtgttt
     7681 gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga caatcccagc
     7741 cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg gttaataggc
     7801 gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa taaaaataac
     7861 ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca acagttacta
     7921 gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga gtgttaccta
     7981 atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta ccatcactgc
     8041 ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt tcttgcttca
     8101 gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca ggaatcgcct
     8161 gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca aacaatcatc
     8221 cagctttttt caattgataa aaaataggca attccggttg atcttcaaac atctgctgat
     8281 aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta ccttgctgct
     8341 tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga tgattcaagt
     8401 aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa tgctcaagcg
     8461 gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt tccattaacg
     8521 cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta gtttcctgat
     8581 tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc tttaccaact
     8641 cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg cttccactcc
     8701 gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag attcggttgt
     8761 tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg agcttcatca
     8821 aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc ggttgtaaat
     8881 ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag aaagatagca
     8941 actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact gtttcttcaa
     9001 tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca tctcaattcc
     9061 taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt tatttaaata
     9121 agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt tttcatcaga
     9181 tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa atgaatacag
     9241 caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact tactctaaac
     9301 tcaaatgaaa aggtaaaaaa actggtagta caatctttta aattctcatt gttttgtacg
     9361 ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa atttttagct
     9421 ctataatttc taagactgat agaaaatcat tctgttt
//
LOCUS       DACCIQ010000099         9285 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCIQ010000099 DACCIQ010000000
VERSION     DACCIQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.101"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            335..1246
                     /locus_tag="IVM62_002382"
     CDS             335..1246
                     /locus_tag="IVM62_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6846304.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1416..1742
                     /locus_tag="IVM62_002383"
     CDS             1416..1742
                     /locus_tag="IVM62_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6846305.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1810..2523
                     /locus_tag="IVM62_002384"
     CDS             1810..2523
                     /locus_tag="IVM62_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6846306.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2833..3684
                     /locus_tag="IVM62_002385"
     CDS             2833..3684
                     /locus_tag="IVM62_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846307.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3805..5670
                     /locus_tag="IVM62_002386"
     CDS             3805..5670
                     /locus_tag="IVM62_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6846308.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5718..7127
                     /locus_tag="IVM62_002387"
     CDS             5718..7127
                     /locus_tag="IVM62_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP6846309.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            7235..7936
                     /locus_tag="IVM62_002388"
     CDS             7235..7936
                     /locus_tag="IVM62_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6846310.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            8095..8574
                     /locus_tag="IVM62_002389"
                     /pseudo
     CDS             8095..8574
                     /locus_tag="IVM62_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         3175 a         1496 c         2024 g         2590 t
ORIGIN      
        1 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
       61 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      121 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      181 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      241 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      301 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      361 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      421 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      481 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      541 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      601 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      661 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      721 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      781 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      841 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      901 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
      961 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1021 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1081 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1141 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1201 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1261 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
     1321 caacagtagt ttaattacta cttgatttag ttctttttta tgtaaaaaaa tggtatgatt
     1381 aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa aaagtcaacc
     1441 agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg gctatctcta
     1501 tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg gaagaagtaa
     1561 aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc tattcataca
     1621 tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa ttgatcaaaa
     1681 ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac acagagttgt
     1741 agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat gagtctggag
     1801 ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat cctcttgttc
     1861 ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga ttattttgtc
     1921 agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct gaaaaagaat
     1981 aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac agaaggggtc
     2041 atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc agtcccttca
     2101 gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact ttacggagca
     2161 ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc aagccatcgt
     2221 gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg tgccaagatc
     2281 tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga aaaagttgct
     2341 cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac actaattact
     2401 tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga aagtgttaca
     2461 tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac aaaaacattt
     2521 taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat gaatcgataa
     2581 ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct gatttggttg
     2641 ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg cttgcataaa
     2701 taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt acggcaggca
     2761 aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt tttcttgaga
     2821 gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat ttcgaaaaat
     2881 ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa aaagattggg
     2941 gacgagattt cgccagaagc ggtccaaaaa gaatttgttt taagagattc ttttgctaaa
     3001 aaccaattcc agcagctgat ggctgatatt ccggcagagg agatcgaatg ggtcaaacaa
     3061 gtagtcgaac tggctgaaga taaactaaaa gttgaatttt cgcctaatat ttatctgaca
     3121 ttgaccgatc atcttcatta tgcgatcaca cgagcaaaag aaaatatcca actgcctaat
     3181 ccattattat ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc gaaagaggct
     3241 cttttgttcg tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc cggttttatt
     3301 gcctttcatt ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac gatgaccgct
     3361 accacaatgg taagagatat actgagtatc atcagtaaat tcttcggcat cgtgttcgat
     3421 gaagaatctt tgaattatca gcgtatcatt actcatctgc agttttttgc ccagcgttat
     3481 ctcaaagggg aaggatctga cgagcaagat gaatttctct acgcattagt tcaaggaaag
     3541 tatccaaaag cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca aacgcaaaaa
     3601 caggaaatgg ggatcgcaga gcagatttat ttaacgattc atattcaaag agtagtaagt
     3661 gagaaaaaaa gcatccaatc ataaggattg ttactgtatg gcaggcaaaa cctggattga
     3721 aaaaataggt tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt attttttaaa
     3781 taaaaaagaa agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg tgtatgggaa
     3841 gcggtaggag gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg gttgcgattc
     3901 agattaaaag atgaatcttt agcagatacg caaaagttaa aagaagaccc ggacgtgatc
     3961 caagtcgtac aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt agcagatgtt
     4021 tatcaatcga tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa agaagagtcc
     4081 aaaaatattt tgaatcgtct gatcgatatt atctctagta tatttacacc ttttctaggg
     4141 gcgatggcag ccgccggggt attgaaagga ttcttatcat tggcaacggt gatgggctgg
     4201 ctgacagctg atagcggtac ttatcaaata ttgtttgctg cagcagatgg tgtctttacc
     4261 ttcttacctg tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa ccagttttta
     4321 gcagtagcaa ttgctatggc acttgtgtat ccagctatca cagcagttgc tggtgcggga
     4381 gaagctatca gtttctttgg gatcccagtg attctttcac catctggtta tacttcttct
     4441 gtcataccaa tcattctagc cgtatgggtt caaagtaaat tagagccatt cgtaaaaaaa
     4501 gtgattccgc aatttctgca aatgatttta gttccgcttg tcgttttagt agtgatggtt
     4561 ccattaacat ttcttgcttt aggaccaatc gggacggttg ccggtaatgc tttaggcggt
     4621 ctcttcaact cgatttatgg atttagtcca atcgtagcag gtctaatcat gggaagttta
     4681 tggcaagtat tcgtgatgtt cggcatgcat tggggattcg taccgatcat gtttttgaat
     4741 atcgaacaat atgggtttga tgtattgatg ccaatgcttc ttccagccat cttggcacaa
     4801 ggtggggctg ctttagctgt agcattgcga acaaaagata cgaaacttcg agcattaggg
     4861 atttcttcta cagtgacttc gttatttgga atcactgaac caactgtgta tggtgtgaca
     4921 ttacctttga agaaaccatt cattgccgct tgtatttctg gaggtattgg tggagctatt
     4981 atcggatttt ccggagtaaa agctttctcc agcagtttgg tcagcttatt gacgattccg
     5041 actttcatta atacggtgga tggtgtagag tcaaatgtga cagtagcagt aatagctaca
     5101 ggaattgctt ttgttcttgc attcgtaggt acattgattt taggatttga cgaacaaaca
     5161 caagacaatc aattagaaaa caaacatgcg aatgcaggag aaccgattac ttcagcacgg
     5221 cataccttaa aaagtccttt aacgggtaaa gtactgccat tgagtgaagt accagaccaa
     5281 gttttttctt ctggcgtgat gggaaaaggt atcgctattg atccagaagt aggagaacta
     5341 gttgcacctg cagacggaga gatcacgact atttttccaa ctggacatgc tgtagggatc
     5401 acaacgactg acggagcgga gattttgatt catatcggga tggatacagt agaattaaat
     5461 gggaatggct ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg agatttattg
     5521 attcgttttg acatcgaagc aattagagca gctggctata gcgtaatcac tccagttgtc
     5581 attacgaata ctgatgcatt tgccgatata cttgaattag accaaaaaga gattattgca
     5641 aacgaagatg ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat cacgtttttc
     5701 agaaaaattt ttatggggtg gagcaactgc tgccaaatca attagaaggg gcttatgatc
     5761 gagacggaaa aggactatcc gttgcagatg ctatgcctgg aggtaagcag cgctttgcga
     5821 ttatcggcag tgaagaattt gactggacga ttgatcaaga aaaatatatt tacccgaatc
     5881 atcgaggaat cgatcattac gatcgtttca aagaagatct tgccttgttt gcggaaatgg
     5941 gattcaaatg ttatcgtttt tcgattgcct ggacacgaat ttttccaaat ggagacgaag
     6001 gtactcctaa cgaagcggga ttagaatttt atgatcagct gattgatgag tgcttaaaat
     6061 atgatattga acctgtgatc acgatttctc attacgaaat gcctcttcat ttggcaaaag
     6121 aatacggtgg ctggaaaaat cgaaaactga tcgacttcta tgaacgattt gctcagacag
     6181 tattagagag atacagcagt aaagtcaaat attggatgac tttcaatgaa atcaattcgg
     6241 cctttcactt tccagcttta agtcaaggat tagtaaaaag caatggagca ggagagtacc
     6301 aaaatatttt ccaagcatgg cataatcaat tcgttgcaag tagcaaagcc gtaaaaatcg
     6361 gccacgaatt gcgttcggat atccaaatcg gctgcatgat tatctatgca acaacttaca
     6421 gcatcgatgc caatccagtc aatcaggcag caacaatgat ccaaaaccaa gaattcaatt
     6481 ttttctgcac ggatgtccaa gtacgtggag aataccctgc atacacagcg cgtacccata
     6541 aaaaatatgg agtcgatccg gaaaaattag agcaaacgga aaaagacttc cagttgctaa
     6601 aagaatatcc agtggactac atcggattta gttattatat gtctacggca atcaatgaga
     6661 ctgatccaca agcagcaact tcagaaggga acttgctggg aggagtaaaa aatccgtttc
     6721 ttgaggcaag tgaatggggc tggcagatcg atccagaagg gctgagaatc gcattgaatg
     6781 aattatacaa tcgttaccag aaaccattat tcattgttga aaacggtctt ggagccattg
     6841 atcatatgga agaggatggc acgattattg atgattatcg gatcgattat ttacgtcgtc
     6901 atatcgaagc gatggctaat gcagtagcag acggtgtaga tttgatgggc tatacgcctt
     6961 ggggatgtat cgatcttgtc agtgcatcaa caggagaaat gagtaaacgt tacggcttta
     7021 tctatgtgga tctagacgat gaaggaaatg ggacactgaa tcgatcaaag aaaaaatctt
     7081 ttgattggta taaagaagta attgcctcaa atggcgtaaa cttgtaatta aagcagctga
     7141 atgaaaaaga acacacttct cacatgtgat ttgtgttctt tttttatttt ttatgtgcta
     7201 ttcttttagt aatagtaaag caaagggtga cgttatgaaa aggacacgtg tactatactt
     7261 ggaagtcgca gataaaatta aagaagatat tttctcagga aaatatccag tcggttcgat
     7321 gttgccgaca gaaagtgaat tagaagaact gtttcaagtc agcaaaatca ctgtccgaaa
     7381 ggcaatcgaa ctgctggcat cagatgaata tgtcgcaaag aaaagtggtc gtgggacgac
     7441 ggttctaagc aatcgtcctt acaatcgatt atccaaagcg gcaagcttta cgcaaatctt
     7501 ggagaactcc cgatatgaag tccggaaaga aacgctggat ttgcaattgg tccatataaa
     7561 gaaggaagat cctttgtttt cctttttcgg tgagcaagct gtttgtttta agcgcttata
     7621 ttatttgaac aatcagcctt atatttattt tgttcattat cttccacaag aaatgatgca
     7681 attcagcaga gaagaatttg gcaaagaatc tttgtatcgt ttgctgacga aaaaaggata
     7741 tactattgac tcattttctg atgatttctc ggcagtattt ttaacagaag aagaacaagg
     7801 aattctgaaa acagacgcac agatcggcat caaacggata cggaaatcgt ctacgcaaat
     7861 tggggaaatg attgaatatt cggaggcggt ctaccatacg gagctgcatc cttaccatat
     7921 tgattatgaa acataaataa aaaaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     7981 agaacagcca aacaaaaata gcttctcata tttttcgttt ggttaggctt attaccgcag
     8041 atacacggct gtttctggat taccatttat tctggataag ggggtgtggc acgattttca
     8101 gaaggaatct atgaaccaat cactgtattg attttagctt cgatggtaac cccattcggc
     8161 ttgacgatcg cttatttctt agggaaaatc attcgtaaaa atatactcaa tcgtcaagaa
     8221 atcgatacat tgaagaccgc ttttcctatg gggatctgtc agatcacaga gggatgtttc
     8281 cctatcgtat tgaacgactt ggcacgcaat gtcatagcaa cgggagtcgg tgggacagta
     8341 ggtggcggat taagcatgtt ttggggcgca gatagccgta ttccggcatc gggaatgttt
     8401 gcagtcgcta cgatgaccag accatgggca ttcattggtg cattgttagc tggttctttt
     8461 gtcacaggaa taaccatttt attattgaaa aaaccagtag atccgaatgc agaaatcatc
     8521 caagaagaaa aagaggaaga agacatatct tgggatgatt tgacaattag ttaggagagg
     8581 cagaatggat tacaagaaaa aagtacaata gatgtacctt gagacaggaa tggtatgaaa
     8641 gaatcagagt tagaaagaat cgaatataca gattttggtt taggagcgat cgaaactgtc
     8701 cagagcatcg gcaccctcct tgaaatgacg atgaaggaaa aatgagaacc tttcgttgtc
     8761 gaaagggtct ggtatacctt atgtcgaagg aaaggaaaca aaaaaacttt cctgtactat
     8821 tccaaaaagg aaagaaagtt attatacagt aaaaaaagaa attattcttc atgctagaga
     8881 tcagtacacg ttcgagccca atatcaaaca ctagttccag gcaggcgcaa caggtgcggt
     8941 tatcactgaa ttttcttcca gcagtgacga ttcaagtgat atttttacaa atccagctat
     9001 ttatcgttaa caaaaagatt tttaggagaa gctgatgaac aaaaatgatc aaattttgat
     9061 cgtttagttc gtcggctttt ttatttagag aaacatcatt tatcagtgaa acataacacg
     9121 agaacaaaat gattttttcg ttaataaaag aaacaagtat ttgttgttta aaataataca
     9181 cgttattggc atataggacg aaatcccagt atcttctata agtaagtcat ttcatatcga
     9241 tgaaaacacg ttatttattt ttgttttatc tataaaatgg ctgaa
//
LOCUS       DACCIQ010000100         9185 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCIQ010000100 DACCIQ010000000
VERSION     DACCIQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.102"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(99..1229)
                     /locus_tag="IVM62_002390"
     CDS             complement(99..1229)
                     /locus_tag="IVM62_002390"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP6846311.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1788..2861)
                     /locus_tag="IVM62_002391"
     CDS             complement(1788..2861)
                     /locus_tag="IVM62_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6846312.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRK"
     gene            complement(2858..3685)
                     /locus_tag="IVM62_002392"
     CDS             complement(2858..3685)
                     /locus_tag="IVM62_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6846313.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(3686..4492)
                     /locus_tag="IVM62_002393"
     CDS             complement(3686..4492)
                     /locus_tag="IVM62_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6846314.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(4504..5589)
                     /locus_tag="IVM62_002394"
     CDS             complement(4504..5589)
                     /locus_tag="IVM62_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846315.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(5606..6148)
                     /locus_tag="IVM62_002395"
     CDS             complement(5606..6148)
                     /locus_tag="IVM62_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846316.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            6406..7395
                     /locus_tag="IVM62_002396"
     CDS             6406..7395
                     /locus_tag="IVM62_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6846317.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7392..8129
                     /locus_tag="IVM62_002397"
     CDS             7392..8129
                     /locus_tag="IVM62_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP6846318.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            8126..8677
                     /gene="coaC"
                     /locus_tag="IVM62_002398"
     CDS             8126..8677
                     /gene="coaC"
                     /locus_tag="IVM62_002398"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP6846319.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            8693..>9185
                     /locus_tag="IVM62_002399"
     CDS             8693..>9185
                     /locus_tag="IVM62_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6846320.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGT"
BASE COUNT         2814 a         1904 c         1582 g         2885 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaatt ctaagaatag gtcattatag atgcctaggt atttcgatcc tagatctttg
     1861 tagacttcaa gatgggagat cgtgtcatca gatggataga attgttcgtc cgacgaaatg
     1921 tcttttggca ataacgcttt tgcttcttca tttggtgtag aataaccaat atactctgca
     1981 ttttgtgccg cattttccgg ttctagcata aagttgatga aatcataggc accttcttta
     2041 tttttcacgg tttttggcat aacgatatta tcaaaccaaa gattggatcc ttctggtggg
     2101 atcacataat gtaaatgttc attttcccac atcatatcgg cagcttcgcc agaaaatgtc
     2161 actgccacgg aggcttcttc attgatcata tacattttga tctcatctgc cacgattgct
     2221 ttgacatttg gtgttaaatg attcaattta tttgctgctt ctgtcagctg tgccatattc
     2281 ttactattca aagaatagcc taaactattc aaagaaagcc ccataacttc tcgtgcaccg
     2341 tcaatcagca tcaaactgtc ttttaattcc ggcttccaca aatcatccca atgcttaatt
     2401 tgatttgcat caaagaattt atcgttatat acgatcccta aagttcccca aaaatatgga
     2461 atcgaatact gattatctgg atcaaaatcc aagtctaaaa atcgtggatc aatattattt
     2521 aatcctttga tcttcgaatg atctaatgga agtactagtt tttctttgat cattttttgg
     2581 atcatatatt cacttggtat agcgatgtcg tagtttgtcc caccttgctg gatttttgtg
     2641 aacatcgctt cattagaatc aaatgtttca taattcactt tgtatccata ttctttttca
     2701 aatttcgtga tcaaactagg atcgatataa tctccccagt tatagatggt aagtaccttt
     2761 gctccactca tgccacttgt tttttcaagt tgacgaacac caagcagcag caccgcaata
     2821 atgatgagaa tacctaaaaa cagagattgt aattttttca tcgcagccgt gccaccccct
     2881 cgcgtactct tctgtttttc ttcagccgtt ttggacggtt ttctttacta ataaagtagt
     2941 atccaatgac caatatcatg gagaacaaga aaactaacgc acttagcgca ttgatttcta
     3001 aactgattcc ttgtcttgca cgagaataga tctctactga gagcgtcgta aagccatttc
     3061 ctgtgacgaa aaatgtaacc gcaaaatcat caagagaata ggtaaatgcc ataaaatatc
     3121 cagctatgat tcctggagaa agataaggca aaatgatatt tttgactact tggaaattat
     3181 tagcaccaag gtctctagcc gcatcgacca ttgaatcgtt catttcctgc aattttggca
     3241 agaccatcaa gacgacgatt ggaatgctga aagcaatatg ggaaagcaat acactctgaa
     3301 aacctaatcc gaatcctagt agtgtaaaga aaatcaagaa actagcaccg ataataacat
     3361 caggagagac taataagatg ttattcatgc tcaacaacgc tgtccgtgca cgtcgttttt
     3421 tcgtgtaata gattcccata gccccaaatg tcccgatgat cgtggcgatc aatgcagata
     3481 agaaagccag catgaacgta ttcaatacga taatgatcaa acgtgtatct tcaaaaacag
     3541 cttcataatt ttcccaagta aatcctgtaa agctgttcat cgttcctcct tcattgaagg
     3601 aataaaaaat cagatagaag atcggtgcat ataagaataa aaagacgatc cctaaataaa
     3661 gatacgacca tttaaatttt ttcatttatg agcgacctcc ttttttcttc tcgcctgtca
     3721 ggagcatcac gatgaacata gcaatgatta aaatcacacc gattgtagaa cccatccccc
     3781 agttttgtgt aaccaagaaa tgctgttcga tagccgttcc taaagtgatc acccgatttc
     3841 cgccaatcag acgagtcagc atgaataaag aaagagaagg aataaatact gcttggacac
     3901 cacttttcac accatttaat gataacggaa aaatcactcg tcggaaggtc tcaaagctat
     3961 tcgcgcctaa atctctacta gcactgatca aagaaggatt catctcttcc aatgcattga
     4021 aaattggcat aatcataaat ggaatctcga tatacgttgc cacgaacaga aaactgaagt
     4081 ctgtgaataa tatctgtttc ggcccgatcc cgataaaatc aaagaaatga ttcacgctgc
     4141 cgtgcacact gaaaatccca ataaatgcgt aagctttaag cagcagattc acccatgtag
     4201 gcaaaataat cagcatcagc cataattgct tgtgcttcag ttttgttaaa aaataagcag
     4261 tcgggtaact gatgatcagc gtgaaaaacg tgatcaaaaa tgcataccat acagaattga
     4321 tcgtcataga aagcgatgtc cccgacgtaa aataagatag ataattattc agcgtgaatt
     4381 gcccattcat atcaaaaaaa gattgataaa tgatcattaa tacaggcgcg atcacgaata
     4441 aaccgagcca caaaatataa gggacggaat agatcctgcg cattgttttc attcttgtcc
     4501 cccctattct tcgtagctgt caagacgtgc atcgaattct tcttcagact cgttgaaacg
     4561 catgacatgg atatcttctg gctcaaaggc caatcctact ttgcttcctt ctttagcttt
     4621 tttcgttgaa tggaccatcc attcattttg ctgttcatca taacagatga tttcataatg
     4681 gacaccacga aacagctgag tatcgaccgt tacgactagt tttcctttat ctggtgtcgt
     4741 gatcgtcaaa tcctctgggc gtaaaacaac ttcaactggt tcatttagac gcatcccgcc
     4801 atcgacacat tcaaattgct taccaacaaa ctcaactaga ttatcttcga tcattacacc
     4861 gtccacgata ttgctctccc caacaaaatc agcaacgaaa tgattgattg gctcatcata
     4921 aatatctacg ggtgtaccac tttggacgat tttcccttta ttcatgacaa aaatctcatc
     4981 actcattgcc aacgcttctt cttggtcatg agtaacaaaa ataaacgtga ttcctaatcg
     5041 ttgttgcaga tcccgcagtt catactgcat atctgtacgt agtttcaaat caagcgccga
     5101 taacggttca tctaatagca atactttcgg ttcatttaca attgctcgtg caatcgctac
     5161 acgctgtctt tgaccaccag acatttcact aatctcccgt ttttcgtatc caggaagctg
     5221 gaccatacgc aaagcatctt ttaccttctt ttcgatatct gatttggaca ttttcttgat
     5281 cttcaatcca aaagcaacgt tatcaaaaac attcatatga ggaaaaagtg cataatcttg
     5341 gaagactgta tttacttgtc gcttattagc tggcaaatca ttcaaccgtt tcccttcaaa
     5401 atagatgtcc ccttctgtag cttcggtgaa tccagctata atacgtagga tcgttgtttt
     5461 accacagccg gaaggaccaa gcagtgtata aaattttcct tgttcgatct caaaacttac
     5521 tttttttaat actgtttcat catcatagcg tttcactact tgatcaaatg aaatgattgg
     5581 tttccccacg tttttacccc ctatattaca aatatgattg cgttgcaaca atcaatactt
     5641 ttgttcgttt tttgagatga ttccttaatt gatgttgttt tgtcgcttga taatagatcg
     5701 actgtcctgc ttttgccaca tagaccgtct ctcctaagct taactctaat tccccttcta
     5761 atacaaaaat aaacgtttca gataatgaag gttcaaacgt cttatattct cctgcacctt
     5821 caaaagtcag caagacaggc tccatttctt tttcattcga atcagccacg agccattcca
     5881 attcataccc gttttcttca tcgaaataaa ccgtatgatc ttcttttgaa taaaccactt
     5941 gcagattcgc tgcttcttga tgaaaaaatt cttctggagt cacacctaag acttcaagaa
     6001 tagaaaagaa tgtttccatt gatggtgaac ttaaatctcg ttccaactgg gaaatatacc
     6061 cttttgacaa atctgtgcgt tcgcctagtt cttcttgtgt caaattcttt tggatgcgca
     6121 aatttcttaa cttctcacca atttccataa tttcctccga atcgaagttt tctaatagta
     6181 aacttcctgt ttactaccac tcgtattagt atacgaaatt tattgaaaaa ggcaaccctt
     6241 tgtaaaattt tttttgctga atatttcttt atcttttgga ataaattcta gttgtttgat
     6301 tctttatttt tcacctaatt tttgtctaaa tttacggaag aacaagaata aaagagtagg
     6361 ctttatggta tgataaaaga aaattttaaa aggagtgtct cacgaatgaa cgcttttcga
     6421 tcttttcagg taataaagga acctgttttc catacagcaa tcagtgatct gccatttact
     6481 gagttggctg aaaatgaagt cttggtaaaa attgcctatt ctgatgtcaa ttataaagat
     6541 gcattggcaa tgagcgagtc tggtcaggtg atacgaacat atccgatgac tccagggatt
     6601 gatttatccg gaactgtcgt ccaaagtaac aatccacgtt tttcaaaaga agatcttgta
     6661 cttgctactg gttttgggct tggcgtcacc catccaggcg gttatagcca atatcaaaaa
     6721 gtaccaggag actggttagt tcctctacca aaaaatatga cgctgcgtca agcgatgatc
     6781 cttggtacag cagggtttac tgctatgttg tgtgtgaacg ctttaatccg tcaaggtatg
     6841 acctccgata aaaaagttgt cgttactggt gcttctggcg gtgtaggcag tacagcgata
     6901 gccctgttac ataaactagg attccagtca atcattgcct tttccagaaa agaagagtct
     6961 gtcacatggc taaaaagtct aggtgctttg caagtcgtac gacctgatga atttctgcca
     7021 gagacaacaa aagctttagg aaaacagcaa atcgactatg tcatcgacac agtaggtggc
     7081 gagcagctaa gcagtcttct tccattgatc tcttataacg gtgcagtagc tttatgtggc
     7141 aatgctgggg gaatcaaact aaacgcgact gtcttgcctt ttattctgcg aaacatccag
     7201 ttgattggaa ttgattctgt caatgtacca atagatcaac gtctttcttt atggcaacag
     7261 atggcggatt tgcagattgc agatgaactg gtcgtccaag agattactct ggatcaattg
     7321 cctgaaacag catctaaact acttgctgga acacaccaag gaagaacact agtaaatgta
     7381 ggtgaccata aatgaagatt ctaatcacag ctggaggaac gactgaaaaa atcgatcaag
     7441 tacgtgctat tacaaatcat tcgacaggac gattagggca agcattagct gattatttag
     7501 ctgccaatcc ggataccaca atcgattacg tgactacgaa acaagcactg aaaccgaaaa
     7561 gacactccaa tataacgatt tatacgatcg aatctgcact ggacttattt ttacaattag
     7621 aagaactaac gaaaaaagaa cactacgatg cgattatcca cagtatggca gtcagtgatt
     7681 ttactcctgc ttttagtttt tcagaagaac agctggcaaa aaacctaccc acttcttcaa
     7741 cgcaagaaga actggacaat tggtttgcgg aaaatgaaca gacagatacc actgtttcaa
     7801 agatctcttc taacaccgag catcttgtgc tcgttttgaa gaaaacacca aaaatcattt
     7861 cttatctacg tgaatggcag ccaaaagcaa aaatcattgg tttcaaacta ttagtagatg
     7921 taccaaagga atctcttctg gcagtcgcaa aaaatagtct gataaataac aaaactgatt
     7981 ttattttcgc aaatgatttg acggaaatcc atggcgagac acaccacggc tatctcttat
     8041 ctaaagatgg aacagtggaa gaagctcaaa gtaaatctga gattgctgca ttgattactg
     8101 agaaaattag actggaggaa tcaaaatgaa aaagatctta ctaggcgtta ctggaagtat
     8161 ttctgcttac aaaagcgctg atcttacaag tcaattcgtc aaattaggtg cccaggtaga
     8221 tgtgattatg tctaaaagca gcacacattt tatcacgcca ttaaccttgc aatctctgtc
     8281 gaagcgcgca gtccatacag acgtcatgca agaatcaaat ccgcaggtaa tcaatcatat
     8341 cgaactagca aaacaagctg acttgtttct catcgcgcct gctacagcaa acattatcgg
     8401 caagctagca aacggcattg cagacgatct tctttcaaca gtcgcaatgg cgcttccagt
     8461 gggaacacca aaattgattg cccctgccat gaacacgaat atgtatcaaa acccgatcac
     8521 tcagcgtaat ctggaaacat tgagatctgt cggctatcag gaaattgaac caagagaatc
     8581 tcttttagct tgtggagatt ttggaaaagg tgccttagct gatattcaga cgatcatcga
     8641 acaggtcatg ctgactctta atgaaagaaa tgcttagaaa aaaggagtac ttatgaaaaa
     8701 tacaaaaaac tttacattaa ctgcgatgtt tttagctatt ttgattttat tagctgttac
     8761 gcctttaggt tttatcccta tcggtccaat caatgcaaca accatgcata ttcctgtaat
     8821 cattgcttcg atcgttttag gaccaagact aggtgctttt cttggcggta catttggact
     8881 gatcagcatg atccgtagca cagttatcca aacacctttg tcttttgtct tttctccgtt
     8941 tatcccagtc atcggaacag aacatggaag ctggaaagcc ttgctgatcg cgtttattcc
     9001 tcgcatcttg attggggtgg ttccttattt cgtttataaa ggtctgcgca aactaatgaa
     9061 acaaaaagca gattccgttt ctttattctt agctggactt tccggttcat tgaccaatac
     9121 gattttagtg atgaatatga tctacttttt attccagcaa gattatgcca aagtcatcgg
     9181 aacaa
//
LOCUS       DACCIQ010000101         8864 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCIQ010000101 DACCIQ010000000
VERSION     DACCIQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.103"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            255..686
                     /locus_tag="IVM62_002400"
     CDS             255..686
                     /locus_tag="IVM62_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6846321.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            693..926
                     /locus_tag="IVM62_002401"
     CDS             693..926
                     /locus_tag="IVM62_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846322.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(848..1090)
                     /locus_tag="IVM62_002402"
     CDS             complement(848..1090)
                     /locus_tag="IVM62_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846323.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            1347..1550
                     /locus_tag="IVM62_002403"
     CDS             1347..1550
                     /locus_tag="IVM62_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP6846324.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            1634..2827
                     /locus_tag="IVM62_002404"
     CDS             1634..2827
                     /locus_tag="IVM62_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6846325.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            3005..3235
                     /locus_tag="IVM62_002405"
                     /pseudo
     CDS             3005..3235
                     /locus_tag="IVM62_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            3363..4046
                     /locus_tag="IVM62_002406"
     CDS             3363..4046
                     /locus_tag="IVM62_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP6846326.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            4355..4428
                     /locus_tag="IVM62_002407"
     tRNA            4355..4428
                     /locus_tag="IVM62_002407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4389..4391,aa:Arg,seq:tct)
     gene            4587..4946
                     /locus_tag="IVM62_002408"
     CDS             4587..4946
                     /locus_tag="IVM62_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP6846327.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            5069..6700
                     /locus_tag="IVM62_002409"
     CDS             5069..6700
                     /locus_tag="IVM62_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP6846328.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6762..7403)
                     /locus_tag="IVM62_002410"
     CDS             complement(6762..7403)
                     /locus_tag="IVM62_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6846329.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7685..7993
                     /gene="rplU"
                     /locus_tag="IVM62_002411"
     CDS             7685..7993
                     /gene="rplU"
                     /locus_tag="IVM62_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP6846330.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            8020..8364
                     /locus_tag="IVM62_002412"
     CDS             8020..8364
                     /locus_tag="IVM62_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP6846331.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            8377..8670
                     /gene="rpmA"
                     /locus_tag="IVM62_002413"
     CDS             8377..8670
                     /gene="rpmA"
                     /locus_tag="IVM62_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP6846332.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         2643 a         1687 c         2060 g         2474 t
ORIGIN      
        1 cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag catagcaaag
       61 cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct tttgcggccc
      121 ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta gcgggtagct
      181 ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt cccccacaga
      241 aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc atgttcgatg
      301 ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa atgaagcgcc
      361 gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac aagctctgta
      421 cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat gacctacata
      481 tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag cagagtattt
      541 taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt gtgggaatgt
      601 cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg aaacagctaa
      661 aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac aagggcattg
      721 agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga gccgtagagc
      781 gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg ctctatgacg
      841 aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg gaaatcaagc
      901 tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa cggcttaccc
      961 tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata tgcccgcaag
     1021 ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag ccggacggct
     1081 ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca cgccgcaatg
     1141 tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc ccttatagcc
     1201 acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga accacgcggg
     1261 ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa atcacacaaa
     1321 cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt tgggaaaaat
     1381 acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac aaattgcgta
     1441 agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac cgcatccaga
     1501 tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa tagtgaaaaa
     1561 gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc gtcatggaaa
     1621 ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt tgcggtctgg
     1681 agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct ttggaaaacc
     1741 gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag gtaaacggga
     1801 ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg acgggataga
     1861 caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca ggcaccgggg
     1921 caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc tggaacaaga
     1981 tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg aatgggcgct
     2041 tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc gttccctgaa
     2101 agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt ttgacttcca
     2161 gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac cagcacagga
     2221 agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt atgacgcaat
     2281 cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga cggacaaaga
     2341 tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa ataccggcgt
     2401 cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc ccatgaatga
     2461 ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa agccgttcat
     2521 tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca tgacggcggt
     2581 agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc atgaagaagc
     2641 gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc ggttagccaa
     2701 tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata tcaccatgac
     2761 gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg aacggctggc
     2821 agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg aaatggtgag
     2881 aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa agcccggaaa
     2941 cacgggctat accgacattc aagaacttct aaaaagataa tctaaaatcc catataattt
     3001 ttcctcaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
     3061 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
     3121 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
     3181 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
     3241 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
     3301 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
     3361 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
     3421 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
     3481 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     3541 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     3601 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     3661 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     3721 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     3781 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     3841 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     3901 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     3961 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     4021 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     4081 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     4141 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     4201 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     4261 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     4321 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     4381 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     4441 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     4501 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     4561 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     4621 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     4681 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     4741 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     4801 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     4861 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     4921 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     4981 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     5041 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     5101 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     5161 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     5221 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     5281 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     5341 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     5401 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     5461 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     5521 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     5581 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     5641 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     5701 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     5761 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     5821 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     5881 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     5941 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     6001 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     6061 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     6121 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     6181 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     6241 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     6301 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     6361 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     6421 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     6481 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     6541 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     6601 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     6661 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     6721 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     6781 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     6841 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     6901 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     6961 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     7021 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     7081 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     7141 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     7201 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     7261 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     7321 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     7381 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     7441 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     7501 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     7561 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     7621 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     7681 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     7741 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     7801 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     7861 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     7921 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     7981 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     8041 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     8101 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     8161 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     8221 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     8281 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     8341 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     8401 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     8461 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     8521 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     8581 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     8641 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     8701 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     8761 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     8821 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcag
//
LOCUS       DACCIQ010000102         8343 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCIQ010000102 DACCIQ010000000
VERSION     DACCIQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.104"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(27..524)
                     /locus_tag="IVM62_002414"
     CDS             complement(27..524)
                     /locus_tag="IVM62_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP6846333.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(654..1364)
                     /gene="budA"
                     /locus_tag="IVM62_002415"
     CDS             complement(654..1364)
                     /gene="budA"
                     /locus_tag="IVM62_002415"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP6846334.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1377..3041)
                     /gene="alsS"
                     /locus_tag="IVM62_002416"
     CDS             complement(1377..3041)
                     /gene="alsS"
                     /locus_tag="IVM62_002416"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP6846335.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3246..3908)
                     /locus_tag="IVM62_002417"
     CDS             complement(3246..3908)
                     /locus_tag="IVM62_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6846336.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3918..4733)
                     /locus_tag="IVM62_002418"
     CDS             complement(3918..4733)
                     /locus_tag="IVM62_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6846337.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4995..5438)
                     /locus_tag="IVM62_002419"
     CDS             complement(4995..5438)
                     /locus_tag="IVM62_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP6846338.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5469..5582)
                     /locus_tag="IVM62_002420"
     CDS             complement(5469..5582)
                     /locus_tag="IVM62_002420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6846339.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(5572..5910)
                     /locus_tag="IVM62_002421"
     CDS             complement(5572..5910)
                     /locus_tag="IVM62_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6846340.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5898..6275)
                     /locus_tag="IVM62_002422"
     CDS             complement(5898..6275)
                     /locus_tag="IVM62_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6846341.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6499..7692
                     /locus_tag="IVM62_002423"
     CDS             6499..7692
                     /locus_tag="IVM62_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6846342.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7858..8280)
                     /locus_tag="IVM62_002424"
     CDS             complement(7858..8280)
                     /locus_tag="IVM62_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6846343.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2450 a         1686 c         1407 g         2800 t
ORIGIN      
        1 cttctttttt gtttcaatag acgattttag ttattaataa atgaaaaagc atttatatta
       61 aaatttgcta ataaaagatc tacatatttt tctagaaatg cttgatcgat ttcatcatac
      121 cctttcgtta ctccgcaatc gatatctaaa acaccgatta aattaccttc ttttaccatt
      181 ggcactacga tttctgattg tgcggctgaa tcgcaggaaa tgtaatttgc atgggttttg
      241 acatcatcaa cgatcaatgt ttcttgtttt tcagctgatt ctccacaaac tccttttccc
      301 atcttgatac gtgtgcaaga aactcgccct tggaacggtc ctaaaatcaa ttcattacca
      361 tcgaataaat aatatcctgc aaagacagtc tcaggcaaag tctcattcaa caatgcagat
      421 gaatttgcta aatttgctaa ccagttatct tctgcttcta ataatccttg ttgctgtaat
      481 aataaaattt cgtatgcatc ttttttctgt ttttctgtcc acatgataaa aaccgctcct
      541 ttttgatcta tggagaagat tatagctcag tttttttgta aacgctaggt ttcctcctca
      601 aaaaaacgag atacgtcagg ttaatcacct tgaacgcatc tcgctgtctt tttctattct
      661 gcttgttgga tttctgctgc taaatcagca taatcaatcg tttcgtgaac aaaagtcgga
      721 tcatcaactg gaaaatgctg tacaagggac gagatgcggc taatctcaac ttttccttca
      781 gccacttcaa aatccattac atgtccgcca aatgtatgtg tatcatcgat aaaatgcaag
      841 tgaaatcccg ctgcagcaac tccttggaat aattcaggcg taaagaaccc gactaatgtt
      901 ccttggacat tgctttgttg gaattctggt tgagactctg aaatttttgc tagtcttctg
      961 taaggttttt cttgcttcgg catgatacgg atgtgcatat ttttgaataa tccagttatc
     1021 ttcactgcca aaaataggtt ttccccggct tctcttacta atatgtcttt tttcaattgt
     1081 tctccatctg cagtttttga gacagaaaaa ctagtatctg gcgtaaaatc tgtaattgct
     1141 gcataggggg taagttcact tccatctaat tgggcaactg agccatctga tcttccttga
     1201 taagctacgc catctaaaaa gatgacttca ccatcaagcc catgcaatgt tcccaatccc
     1261 agagttcctt tttctaatat atgagcaatc gtttctgttc catccatcaa gccagccatc
     1321 aaagctccta atgtcccgtg ctggtataag attttttcag tcatgtcgac acttccttaa
     1381 tagaattgat ctggtaagat tgtttctccc aatttctcat tatcacggta atcaatcgga
     1441 atatcaataa ttacgggtcc ttctgtcgca aatccttctt ctaaagcttt ttctaattcc
     1501 tctacacttg tagctcggat tcctttagcg ccaaaagctt ctgcatattt cacaaaatca
     1561 actggaccaa agtctacacc tgacgcccgc tgatatttca ttttttcttg gaactctacc
     1621 atattgtagt gtccatcatt ccaaatcaaa tgaacgatat tcaatttttt acgtaccgcc
     1681 gtttctaaat cctgggcaga gaataaaaat cccccatcgc cggaaactga tacgatttgt
     1741 gtatttggac gcaccaatgc agcagaaatc gcccatggca atgcaacacc aagcgtttgc
     1801 ataccattac tgaataacaa atgacgaggt tcataacttc taaagtggcg tgccatccaa
     1861 atataatgac tacctacatc tacggtaaca gtcatatcat ctgttacttt tgactgaagc
     1921 gtgttaataa cttctaaagg atgcaagata cctgcttctc cttttgattg aacaatgtcc
     1981 cgctccgtta attctgcttg aagagatgct aaatattctt ttgcatcttc tgacacttgt
     2041 tgaggctcta atgaacctgt caataaatca agagtggctg agatatcacc gatcaattct
     2101 ctttcaggct gcataaaagg atcgatttca gctggcgctt catcgatcac aatgatccgc
     2161 gcatcttttt ccgcattcca gtttcttgct tcatattcga ttggatcata gccaatagca
     2221 ataactagat cacttcgttt caaaagcata tctcctggtt ggtttctaaa taaacccaca
     2281 cgaccaaaga aatgcgcttc taattcacgt gagatcactc cagctgcttg gaatgtctct
     2341 acaacaggca atgctgtctt tccaactaat tgcctgatgg ctaacgtttc tttttcactt
     2401 gatcctcgca tacctactaa tagtaccggt agttttgctt ctctaatgcg ttcagctagg
     2461 taacggatat catctgcaga agcagaacct aatttaggat cgctcaatgg cttgatgaca
     2521 ttcccttgga caggtgcatc cactacatct tgcgggatac tgataaagct cgcgcctttt
     2581 tttgaactct tagccatacg ataagcattg gcaatcactt ctgaaattgt ttctggatct
     2641 tggatttctg cactgtattt tgtgattggc tggaacagtg ctgcattatc catgctttga
     2701 tgggtcaact tcaatagatc gcttcgtttt acttgtccgg caattgctaa aactgggtca
     2761 ccttctgctg tcgctgttac caaacctgta gctaagttac tagcaccagg accacttgtt
     2821 gcgataacta ctcctggctc tcctgtaata cgtccgattg cttgtgccat aaatgcagca
     2881 ttttgttcgt gacgagtcac gattaattca ggaccttgat cctctaattc attaaataca
     2941 ccatcgattt ttgcacctgg tataccgaat acataatcaa catgatgatt aatcaaactt
     3001 tctactacag atttcgaacc ttgtttcatt ttttcgtcca ttgaacatcg tcctttttat
     3061 gctttatttt taattattag ctagaaataa tcacaaataa ttgatacagt ttctaaacct
     3121 gtttcattca aaagtatagc acgttttatt tcatttgttt agccatatgt ttcaaaaaat
     3181 gaaaaagaat gacagttttt acataaaaac tgtcattctt aattgaaagt ttcaatttat
     3241 aaagtctaca gatccatagt tctcatcaat ttacgattgt tgaagtaaat caaaccaaat
     3301 acgatagaag gacctagtag aatacaccat tggggcttta cgaaaaagaa gagaaacaaa
     3361 cttgtcaata gtaaaaaatc tagtaaactc acataccatt ttttcaacgt atagtagaac
     3421 gctatccgta gaacttgatt tggtttcgac gctggatttc tcacctgaaa ataccagtag
     3481 ttgatgctaa aggaaactga taaacaagca agtaaaacaa acaacggaat caaccatttt
     3541 ccataagaaa aacgtataaa aaagaaacaa tcaaaaatca ttatcacact aactatccaa
     3601 gcaaatagcc aataaaaaaa tccctttagc caaaattggc gaaacgtctt tatgaacaaa
     3661 cgtactggtg aaaaactttt ttcttttgct aattcttctg tcatacctat catcacgatg
     3721 caacctggac caataaaaag taaagaaaaa ataaaggaaa agacattcct tccatctatc
     3781 gacaaaagga acgcagctgc tatatacaag atattgacac accaaaaaca aaggctactt
     3841 gccaatgatt gatacaacca ataaaagaat tccatataaa catttttgtc aaaacgctgc
     3901 tctttcataa tcacctccta ttccttcatt cctgatgaag cgatcccttc tacaaaatat
     3961 ttttgcatcg ctaaaaatat aaaagcaaca ggaagaacag aaacaactga ggcagccatg
     4021 attaatgcgt actctgcatc aaatagccca ataaacattt tcaaccctaa ctggattgtt
     4081 ttattttccg tagaagacaa ataaatgaat ggccccatat aatcgttcca tgtattaacg
     4141 aatgtgatga tcgtcaaact ggcaatcgcc ggttttgtaa gaggtaaaat gatcttgcga
     4201 tagattcccc actctgataa gccatcgatc cgcgcactct cacataaaga atctgggata
     4261 ctactgtagt attgcttcaa caagaacaca ccaaaagcat tgaacgcttg taatagcacc
     4321 aacgaccaca acgtatatgt taaacccact tttctcatca taatgaactg cgggatcata
     4381 taagactgcc aagggacagc aatcgtgccg atatatgcta aaaataaaat atcgcgccct
     4441 ttaaaattta gtttagaaaa cccgtaagca gcaaaactag aagtaaacaa ctgaatcaac
     4501 gtgatcacaa cgcttaaaaa agctgtattc ttaaaaaatg tcactaaagg aatacgagac
     4561 caaataacca gataattctc taaatgccaa gttttaggga tccattgaat cggaatcgtg
     4621 aacacttcat tatttgcttt gaatgaagca gaaaccatcc ataaaaaagg aatgatcgtc
     4681 aataaagcta atacaatcag cagaatcata cccaaccatt gaataaaggg catgtttttt
     4741 gaacgaattt ttgctttttt tctttcattt cgaacacctc caaagttata atcttcattt
     4801 ctttgtaatg attacaatcc agaacaacgg ttattatagc tgattcattt ccgttcttct
     4861 tatgtttgta agcgcttatt taacaagtta tgtaaatctc tttatttttt ttgatttaaa
     4921 aatgaaaaaa tacaaaagca agactgtcat acgtctcgct tttgtattga aatttcttcg
     4981 attagagata cttattactg ttcaaaagct tctttgaaac ggtgaatcaa ttcattgact
     5041 acaggtgata aaatccgatt ctttttccag actagcatgc agttcgtttc aatcattggg
     5101 tcaagcggaa cgaatgtcac tcgctttgaa agatgactta cagcaccttc tatgactaaa
     5161 gcagcaccta cctgattctc aattagcgca aatacattga aaatcaaatt gtacgttcct
     5221 gtgatcagca actgctcctt ttttatgcct aaccagtcag caaacattcg ctgtatttct
     5281 ggacgctttg agatcaaaag aggtacattc gcaaagtctt cttttgttat cacgtctttt
     5341 tttgctaaaa cagaattttc taaaatgagc aatccccatt tttcctttct tggtaacaaa
     5401 atcgtttcgt atttctctgt atctactgga tctagcaaaa tcgctaaatc taacaggctt
     5461 ttttctagtc actccgtgat atcatcactt gttccgctgt ggatatgaaa ttgaacgctg
     5521 ggataatcac ggatcaactt ttctagtaat agtgccaatg tattcgaatt atcagcttcc
     5581 acacagccga tcgtaaatcg gccgctgaat aattgttttt ttctgttctc gaattcttga
     5641 gtggtttgat tcgttaaatc taaaatttct tctgctcggc ttttttagaa aataacctgc
     5701 ttctgtcaag atcattttgc ggttttcgcg tacaaacagt tctgttccta gttcctcttc
     5761 taactccttc agttgtcggc ttaaggttgg ttgtgtaaga tgtagaactt ctgcggcttg
     5821 agagattgtt ccttcttctg caattgtcca aaaatatttt agtaagcgta actccatagc
     5881 gatgctcctt gattttttta cttgatacat tccattttat ttctagataa cacacgatca
     5941 tatttcttga tttttccttg taaacgttcc aaacattctt gcatttcagc aatttttttc
     6001 gtcaaaagtt cactttcttc aatcaaaagc ttacgccgct caaatacagt tgcttctcct
     6061 tcgctagata ggcgaatata ttcaatcaaa gactcgatcg ataatccggc atttcgcata
     6121 cagatcgtaa agtcgaccca ttttaggtct tcttctgtat aatcacggat tccgcctttt
     6181 gtccgattaa ctggtggaat caacccgatt cgttcataat agcgcaatgt atcggccgaa
     6241 atgccctttt cttcactgac ttgtttgata ttcatctgat cactctttct cttaatttct
     6301 tgccacttta cttatgtatt ttatttattc tacaacttta agtgtactcc aagtcaatac
     6361 tcatttctat taaaactaag ctggattatc tatatcggtt gtttgtgtat tatttcaaat
     6421 aaaaaagtct tgttttttta ttcttttttg gatatacttt taacaattta gggaaatttt
     6481 aggaaaaggg gtataaaaat gacaacgtat cagcgaaagt tattattatc gacatcggcg
     6541 ggaattgctt tagagaatat ggatattatg tttttagctt tttcattatc ttcaatgatt
     6601 acttcttttc atattagtgg tacgcaagcg ggtttcattt caacgatcac taatttgggg
     6661 atgctcattg gaggtatttt ttttggcatc atagctgata aatatggacg tgtgaaagta
     6721 ttttctcaaa cggtccttct gttttcgatt gcttctttat tgatgtattt tgcttcaaac
     6781 atctacttgg tttatctctt tcgctttatc gcaggtgtag gagcgggagg agaatacggg
     6841 gcgtgtatgt ccttgatttc cgaaagtttt tcaaaaaaac aaattggacg agcttcttcg
     6901 gttgctggta ttggtgcgca ggtcggtgcg gctttggctg cgattttagc tgctgtgatc
     6961 attccaacgt ttggttggaa gatgttgtat gtcgtaggtg tactgccggt gttgatggtc
     7021 ttgttcattc gtcgcggctt gactgaacca aaggaattca aatctgtaca atctgccgaa
     7081 aagaaaacca aacttagcag actgtttgaa tcaaaacatc ttgcttggca aacatgtggc
     7141 cttagtatca tggtaactgt ccaaattgcc ggctattttg ggttaatgaa ttggctgcct
     7201 tcaattatgc aaaatcgatt agggctttcg gtttctgggt cttcattgtg gatggtcagt
     7261 actattttag gcatgtcttt ggggatgatg acttttggca taatcatgga taaattgggg
     7321 cctaaactag ccttttcttt gtttttaatt tgttcatcct tatctgtcta tctacttgtg
     7381 ttagcccata gccagtggag tttggtagca gccgctgtga tagtcggcta ctttatcaat
     7441 ggcatgtatg gtggatatgg cgcagtgatt agtagtttgt atccaacaga aatccgcgcg
     7501 acggcgaata atttcattat gaaccttgga cgagctgttg gtggtttttc ttctatcgtc
     7561 atcggtttct tgatggatca ttatagttta actgccgtaa tcttgttttt gagcggtatt
     7621 tacctagtca gtctgctggt cttgacaact cttactggtg ttacagcatt aagggaaagc
     7681 ttgcagcatt gatagttgag tctgtaaatg acaagaaaga ggtgtggcaa aagcttgcat
     7741 ctgcggtaat aagaccagcc aaatcaaaaa catgagacac tgtttccgtt tggctggtcg
     7801 tattacttga tgcagggcag ctttttcaac tgctaatttc cttctatctg gcggctacta
     7861 ttctttgacg tccgtcattt caaagtactt tactaataac ttagcataac gaaaaactgt
     7921 ggatacactc gtatattgag cgtctgcata atcttccaaa tttttctcag gatgttgata
     7981 catgaattca atcaaatgat aattatatga ttttctgtag aaatgaagat aaataatgta
     8041 gattgggaag ttctcctgct tttccataga aaagcgccga ttattctcat tcttcaaaat
     8101 gaccttatca ggatattttt ggaacaattc ttctaaatct gttagataat tcaagataga
     8161 cttacttgat accttcaatt cgttttgtag ttcttctatt gaacaccaac gttctgtttc
     8221 aagcagtaaa tgaatcattt taattttctt ttgagaaact tttgttaaaa aagtctgcat
     8281 attttctcct cctgtatcta ctttactata ctacatacaa gaaaaaaaac ggatacaaat
     8341 atc
//
LOCUS       DACCIQ010000103         8104 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCIQ010000103 DACCIQ010000000
VERSION     DACCIQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.105"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(82..930)
                     /locus_tag="IVM62_002425"
     CDS             complement(82..930)
                     /locus_tag="IVM62_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6846344.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1154..3094)
                     /gene="thrS"
                     /locus_tag="IVM62_002426"
     CDS             complement(1154..3094)
                     /gene="thrS"
                     /locus_tag="IVM62_002426"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP6846345.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3431..3610)
                     /locus_tag="IVM62_002427"
     CDS             complement(3431..3610)
                     /locus_tag="IVM62_002427"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP6846346.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3657..5480)
                     /gene="cadA"
                     /locus_tag="IVM62_002428"
     CDS             complement(3657..5480)
                     /gene="cadA"
                     /locus_tag="IVM62_002428"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6846347.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5758..8037
                     /locus_tag="IVM62_002429"
     CDS             5758..8037
                     /locus_tag="IVM62_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6846348.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2380 a         1715 c         1465 g         2544 t
ORIGIN      
        1 gaaacacttt gatggatcat ttatttgtgg aaaactacgt tttgttattc taaaaaactt
       61 atataaaaaa tagtaaggtt tttatttcga atccataaaa tattcttgga ttgcttctcg
      121 tgtttttttc actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat
      181 atcgatcgta gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc
      241 ttttggtcta gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa
      301 ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac
      361 atctgcactt ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac
      421 aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga
      481 ccatttttgc agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac
      541 agtctctgcc gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc
      601 tactgtttct tttatcgcta cttgtgcatt gttaccaagt ttgtttttga gagagtaaaa
      661 ggactcattt gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga
      721 aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac
      781 tgtcggcgca ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt
      841 ctcaatatta ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat
      901 catactttca atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta
      961 tgtaagtata ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa
     1021 caatccgccc aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg
     1081 tctttgagga atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca
     1141 ggaaatttac ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa
     1201 taaagatatt caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg
     1261 ttgcatcttc caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag
     1321 aggcacgtat cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct
     1381 tcatttgcaa acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag
     1441 ggatgattgt tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg
     1501 ttaaataagc gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac
     1561 gatgcgtatt ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga
     1621 agtctaattg tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat
     1681 ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt
     1741 catccatagc ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat
     1801 atttatctgt attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga
     1861 agtcagcata tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat
     1921 caggacgaac aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg
     1981 acaaagcacc tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta
     2041 attcacgata agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg
     2101 ggcgaagaac aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat
     2161 aatgatccca gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata
     2221 catgttggta acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga
     2281 ttgttgcccc ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga
     2341 acaagtcaag ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac
     2401 ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca
     2461 tttgattgtt tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt
     2521 gtatacggcc agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt
     2581 gataagcagt aatcacttct tcttctggca attcactaat cagttctact ttgtacggat
     2641 cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat
     2701 tttctttgac gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt
     2761 ggttttgtcc attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa
     2821 agtggatatt tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac
     2881 ggactaagcc aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct
     2941 cgattggacg agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg
     3001 ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt
     3061 tgactgcacc atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg
     3121 attgatcaga tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac
     3181 taggacgaaa agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt
     3241 gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt
     3301 ctcctggaaa agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg
     3361 gctttctcat caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg
     3421 gaaactaaac ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat
     3481 tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga
     3541 tatctttcat catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta
     3601 cgaatggcat aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat
     3661 actatgtttg tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag
     3721 gcactcaaaa tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg
     3781 attagtcccg tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca
     3841 gactgtttcg ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag
     3901 agatcatctt tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc
     3961 cccattgcaa taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct
     4021 accatcgcaa caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga
     4081 ggcaagctac gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg
     4141 gcagatccat ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg
     4201 attgtctttg ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat
     4261 cgatcaattg ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa
     4321 gaggcacgtc caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc
     4381 acttctttca gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa
     4441 cgagctaaga tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct
     4501 ttttttattc cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt
     4561 cctgttttat caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt
     4621 tttacaacga tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca
     4681 gctagaatca gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct
     4741 tttgagaacc accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca
     4801 taacgatcag ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta
     4861 ataatcattt gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg
     4921 tctccattta aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac
     4981 tcacctgtca aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg
     5041 ggtacaagtt cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact
     5101 atatcctgca attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa
     5161 gactgcagtt ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta
     5221 agcatgatca atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt
     5281 gcggtaatcg ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc
     5341 aaaaacatca agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc
     5401 ggctggtgaa aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa
     5461 aattttttga aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta
     5521 ataaatttcc agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac
     5581 aataggcctt aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac
     5641 aaacaaaaat attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt
     5701 cgaaaaacta tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg
     5761 aatgagaaac aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa
     5821 caaaatatgc tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa
     5881 gacacagcta atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt
     5941 gaatatcgcc aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa
     6001 aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt
     6061 aaagaagacc gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca
     6121 gaagaaacaa tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag
     6181 attggtcccg cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa
     6241 cgacaattta aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc
     6301 aatgatgact ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc
     6361 gtctctacga tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg
     6421 ctgattgaag gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt
     6481 ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt ttcacccaac
     6541 catttgtttt ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct
     6601 acacagacat tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa
     6661 cagcaacaag aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc
     6721 ggattgacct tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc
     6781 ttgttccgtg atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa
     6841 tggtataaag aaacaaatga acaattacct ttacatcata gactttctct attacaaacg
     6901 aagctcctga aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa
     6961 gatctggcgg aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca
     7021 gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc
     7081 cgtcgaatcc atcgagggat catgaattat caatttgtca atataacgaa acaatatctc
     7141 cactttctac aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg
     7201 tggcaggaac atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag
     7261 gatgctgtac tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa
     7321 gctcgctata tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg
     7381 caagccttgt tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt
     7441 ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt
     7501 ttccaattaa cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt
     7561 attgtgaaag aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc
     7621 caaggaacaa cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag
     7681 ggaaaagccc ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct
     7741 ctttatgctc aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt
     7801 gactttatga agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt
     7861 gaccgagttt tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg
     7921 atcctttaca ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat
     7981 gaaagtcctt tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta
     8041 aaaagacaac tgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca
     8101 gaaa
//
LOCUS       DACCIQ010000104         7881 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCIQ010000104 DACCIQ010000000
VERSION     DACCIQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.106"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            171..515
                     /locus_tag="IVM62_002430"
     CDS             171..515
                     /locus_tag="IVM62_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP6846349.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IVM62_002431"
     CDS             516..935
                     /locus_tag="IVM62_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP6846350.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="IVM62_002432"
     CDS             complement(1027..1377)
                     /locus_tag="IVM62_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846351.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IVM62_002433"
     CDS             complement(1343..2674)
                     /locus_tag="IVM62_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP6846352.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IVM62_002434"
     CDS             2930..3496
                     /locus_tag="IVM62_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6846353.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IVM62_002435"
     tRNA            3641..3728
                     /locus_tag="IVM62_002435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IVM62_002436"
     CDS             4062..5036
                     /locus_tag="IVM62_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6846354.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IVM62_002437"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IVM62_002437"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP6846355.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..>7881)
                     /locus_tag="IVM62_002438"
     CDS             complement(7451..>7881)
                     /locus_tag="IVM62_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6846356.1"
                     /translation="DFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIAIDQAKK
                     WYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKSKGAKKA
                     SNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         2750 a         1323 c         1507 g         2301 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc t
//
LOCUS       DACCIQ010000105         7848 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCIQ010000105 DACCIQ010000000
VERSION     DACCIQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.107"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..1481)
                     /locus_tag="IVM62_002439"
     CDS             complement(<1..1481)
                     /locus_tag="IVM62_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846357.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDA"
     gene            complement(1501..1668)
                     /locus_tag="IVM62_002440"
     CDS             complement(1501..1668)
                     /locus_tag="IVM62_002440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846358.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1705..2394)
                     /locus_tag="IVM62_002441"
     CDS             complement(1705..2394)
                     /locus_tag="IVM62_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP6846359.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2649..2969)
                     /locus_tag="IVM62_002442"
     CDS             complement(2649..2969)
                     /locus_tag="IVM62_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846360.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3002..4120)
                     /locus_tag="IVM62_002443"
     CDS             complement(3002..4120)
                     /locus_tag="IVM62_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846361.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4149..4943)
                     /locus_tag="IVM62_002444"
     CDS             complement(4149..4943)
                     /locus_tag="IVM62_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846362.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(4953..5828)
                     /locus_tag="IVM62_002445"
     CDS             complement(4953..5828)
                     /locus_tag="IVM62_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846363.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6045..6302)
                     /locus_tag="IVM62_002446"
     CDS             complement(6045..6302)
                     /locus_tag="IVM62_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846364.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6305..6529)
                     /locus_tag="IVM62_002447"
     CDS             complement(6305..6529)
                     /locus_tag="IVM62_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846365.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6702..7745)
                     /locus_tag="IVM62_002448"
     CDS             complement(6702..7745)
                     /locus_tag="IVM62_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP6846366.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2189 a         1625 c          989 g         3045 t
ORIGIN      
        1 atcgcatcac acgtattcaa atttttaagt atctttccat tattttcgct tacatcaaac
       61 attttaatat ttggtaatag ttcagttaat ttattttgaa aatttacatg tcctcccact
      121 aaaagaatgt ttttatgttt tagataagct atcatttcag acagtgaagg ctcttcagtt
      181 ttaccattta atgattgtct atgttcttgc tcctgcaatt gctttaatac tttattttca
      241 aacctcagct gttcaagttc ttttctgatt ccttttaatt ctttaatctc ttgcttcaaa
      301 agttcctctt ctttttttgc cgatttgact aaagattcta accgaccgtt ttctttactc
      361 gcaatactta attcttttct taatttgatt acttcagatt tgttttcttt tctaatactt
      421 gcaatttgtt ttgctatttc ataatcagaa ttcaggatca attcttcctt agcattttta
      481 aacaaccgag ttaaaacgaa ctgatataac aaactataaa aaactagcaa atagtcaatt
      541 tgtcggtact cggtattctg atcaattgca tatagagaga ttaaaacgaa atacacaata
      601 tcattcagct cgctaccctt gatctcaaaa ttattgacta agtaactatc aatacccatc
      661 aaatctgcaa aatctccata caaagaaaaa tatgattttg acaatatatc tatatctgaa
      721 tcgtcaaacc catttactat tttcccatca ttgttagtag ttgctaacga agcaatagtg
      781 acttttgaat tgggttttac aaatcctata cctaatagtt ctttcatctt tttaatttca
      841 agtttgttat ccttttgttc ggctttatgc tgtttcatat tgtaaagcgg ataaaatgct
      901 gttccgtaag caccttttag tacgttatct ataaaataat cttcagaacc tgttttgaat
      961 agaaagttaa tcgcagccaa attagataac agctcttcag tgttgtgcat caatttttta
     1021 tcatttaata aattatcccc aagatatttc attgcataat aagggtttgt ttcgctactt
     1081 atttttaata ttttttgata gctattcctc acgatattaa ccaaaatatc tgtctgatta
     1141 attgaattac agtacaaagc cactcctaat gccatataaa cattcaaatc atgttttgcc
     1201 gtaccgttga ataaaacgtc ttcaactttt gaattatctc gtctatactt tttgatcata
     1261 tttttgatat tttgatcatt ctcttggtat atttttttca ctcgttccaa tgccttgggt
     1321 ttgttggaaa cagcaacagc gaagtctagt tctttgaaat caatatttag tatctgtacc
     1381 tcatcaccta gattataatc tctatttacg ctcggaaaac tgctgaaatc taactgtttt
     1441 ttacctattt ttggcgaaaa tattccttcg atttctggca aatgtaatca cactctctcc
     1501 ttatttatct ttagaaaatt tgtcactatt ctccttatat ttatcaataa acctttttaa
     1561 tcgagcaata taagcgttaa cccttttagg ttcttctttt cgccattcat cgtttacgtg
     1621 accattttca aagaaagaag ctaaaagagt ttccacttca ttaaccatat ctgcgatcgt
     1681 atatttttct gtaccctcac tcaattattc atgaactgtt aagtgttcat agctaagttc
     1741 attcataatt ttgttatagc gttttcttgc tttttcaaat tctttctgtt cgtcaaattc
     1801 cacttgattc ttcatgttgt tcttcctctc tgtttgatat tttttcactt cttcaagttt
     1861 agcatctagt tccgctttaa cttctgtcac ttctttaagc gcttgggata acttatgaga
     1921 caatttcgca taagtttctc tcttgtattc atttcctact cccaactctt cggagtagaa
     1981 ctcttctagt agtcttgcaa aattaacatt tctcctgcca gcatagtttt ttagcttctc
     2041 aaaatcagat tgaaagacag caacactttt gtaatccttt tcaccctcaa aatcaaaagc
     2101 atagtcatac tttcctgaaa tcaattcatc aataactgta gcaatatatt gagagataga
     2161 aatattatct tcacttctat gtcgctctat aagattatat atatcagggc tgacatgaaa
     2221 cgtacttctc ttttttgttt gcctttttct aactgttacg accacttttg gaacttcact
     2281 atctccattg atctctttgt atttttcaat aaataatttt ctagcttgtt ctcgactttt
     2341 tgccggaatt tcatctacaa ttttatcttc taaataaata tagtgtgttc tcatacattc
     2401 gtcctctatc ttctttactc tcaatcttca tgatcttagt tcttaccttg tttactctta
     2461 aacattgcaa aaaaatctag tcaaaatcaa acttgaaatc atcgtctatc acaacaccct
     2521 taacttcatc tttagatttc ccaaaggaac ccttattttc aaatccttta tttttgaatg
     2581 atgatggtta tggaaaactt cctttgaaat tctgacaagc ataactagtc agaactgttt
     2641 tacttagatt aagctttcaa aagatactga tgaagaacta agttctcagt accttcccat
     2701 tcttcaataa aaaatatttc tccttctttt acccactcta tagctaattt cttataatca
     2761 aataaaccat atgtgtttaa gcctttgtct ttcattaaac tcaaaaactg atcccctttt
     2821 aatctttctg tatcatccat cagaaattca actaaatctt tatccatgcg tgcaactttt
     2881 tcgtctttta gtgccattga tttagcatag tcagttgaat cttttagaat aataacacct
     2941 acatttcctt ccacaatttt cttcttcata tttacctcct accatctttt ccaaagagta
     3001 tttagtcaaa atcaaactcg aaatcatcgt ctatcacaac acccttaact tcatctttag
     3061 atttcccaaa ggagccctta ttttcaaatc ctttgttttt aaattctaaa gctttttcga
     3121 cttcttcttt ggatattgta tgaacttttt tcttaactac tccaagttta tacagctctt
     3181 caaactctgg tacttcggat ctgaaataca atccatcagt acgaattttt aatagcccat
     3241 atgcgctatg aacgtatttc tgttcatatc taaccatacg atctctaaat acgcctgcta
     3301 ccatggagga atacgctcga ttatacatac tgtatttatc gaaggtgctt gtttctcctt
     3361 ctcttaatga tttttgtccg atagcaccaa atactttcgt tctaagattt ttataaagct
     3421 tcaacgtatc aggatcagtt tttaatggct cgattctttc aaaatattct ttgagtaatc
     3481 cttttttcgt caaaaatgtg tggctatcta ggacttccat ctctttaata tcataataat
     3541 caaggataag tgttagctca atctccgata accaaatagg ctcattagct gttttgggat
     3601 tttcgccata atccgcaaac atcgactgtg attttggatt agcatatgtt ccttctttgg
     3661 ctgttccttc aaaattaacc aaaaagaaca tatacgtttt taaattttca tacggtctta
     3721 cttttggtct atttatcgct ctcaaaatag cttgatctgt attatatttt gctttcaaat
     3781 aagtgtttga cggcaaagga taatttctca tgatcctcga ataggcttcg ttgaaatcat
     3841 attcgatcat ctttgctcct tcctcaaaca aatccaagtt atacatgcta ccggaggtac
     3901 tggctctata ggctgaactt ggaacaaaga aaggtgcatc ttccggctta tcatagccca
     3961 aaaaatgtat tcctgtttgc ttgttctgac ggtaaatgtc catcaaaaga tatttgatcg
     4021 tactaaaaga atctaatttt cctcttgtca tccaccctaa tccgacaagt cttttaattt
     4081 tattctgcat ttcaggtgtg tatatgctat tgtttcccat ttatctgctc atctccggct
     4141 tttttattct agaccctata tttgttcatt acaccactta tctcgtctac aaaggctccc
     4201 agatcgtgtg taaaggcatc atagtaatct tcaatctcac tcgattcagt tctccggttt
     4261 cttccgtttt tatttctgaa tagatacatc tgcgtttctc ttaaatccct tgcttttttc
     4321 gcaagtgcta ataaatccgt aaaattatca ataaccataa tgtcattgat cataaaacct
     4381 tcttcatgac catacttttc agccatctcg ataaaatcat taacatcgaa caactccaat
     4441 aattctgctt tatatttctc taacaatgta tttaaatagc gatttctcaa ttcccatgaa
     4501 ctttcaccag ttccgtattc aaccttgatc cctaaattct tagtgtagcc ttttgctgtg
     4561 tatcgtccaa tgaagtctcc cttattccga ttgctatctt cttctttgaa aaactcacgc
     4621 caaatcccct ttttcttctt aggaattaga cctctgctct tgaaatcatc atattttttt
     4681 gaagcttctc ttcgtgtatc taagtacctt tgcttcagct ctgtagctga ttcattcgtt
     4741 ccatattcaa cagtgatcct taagcgcctg ctataacctt ttgctgtata tcgtccaaca
     4801 aagtcaccgc cacttcgatc actactgact tccttaacaa attctctcca tatacctttt
     4861 ttgtcctttg gtattaatcc tcgtcttttc atatcttgat atttttcaga agcttctttt
     4921 agagtattct tttttgctac catatattcc actcactctt tgttatctag tattttaata
     4981 actggtttca tttttgtaat atcttctttt tcagctatat acaactccgt tacggtaacg
     5041 atcttagtat gatttagatt aattactttc acttgatcgc caattttgat ttttcctcgc
     5101 aaccgtttgg gaataaacca gttgtataat cgtgatttat tattatctac ataagctttt
     5161 attgcatttt caggaaacag gttatttagt ctttcaattt ttttactttt aaaaaattca
     5221 ttcgcaaact tttcatcaac aactaaatta aataaagttt cagaatttac agcttccata
     5281 tctattcctt tgtagatagg agaaagcaat tttttatatt ttgccagtaa tgactttcca
     5341 gaattactaa tcttaatttg gttacttaaa tcttcttgat aaagaacccc atatcgaagt
     5401 aacgccttaa tataatgttt taatatgccg ttgataccag aataaatatc catgccgttc
     5461 tcaatatagt agaaagtgtc gtctctccaa aacaatttga agctattatt caaataaagt
     5521 aacaagatca atatatgttt gtcagaaatt ctgacaaatc tattactttt tagatactca
     5581 ccaatggctt cacttaactc tttgaagcca ttgtattttc ccgataaaaa tgtcgtaaca
     5641 ttatcttctc tctttttcct atggtgtgaa cttaccctga aataatagag tctattctca
     5701 tttaatattg aaaaataaat actttcagtt gtttgtgagc attgtatata gttgaaatga
     5761 tatccatctg gcatgacttt tttaactgta ttttgtacta gctctgacca ccttttgtta
     5821 gtagtcatat atttttttcc attaaaccaa acataaatat cttttttaaa aattttgttc
     5881 cccccctatt gcgttttctt tgacattaaa gccctttgtt ttcatcacat tcgtatagcc
     5941 ctagccactc ataacctgat gcttttgcga agtttattta aaagtgtttt cctttgtaat
     6001 ctacctttcc cctcgtcctg gtacttttct tggcgactat taaactattc aactttttta
     6061 gaatcattgt cagcttgaaa tccttcacgt ttcattatgt cttctgcttt tccaatgctg
     6121 attcctttcc aatcacgcca tcctacgctt cccttatttt gacgataggc tttaatagct
     6181 gattcggata tttgccatct tgtagccaac tctttggctg tgtacttatt tgaaaaaagt
     6241 agcttctcta tcagtgaaat atcaatcatt ttattctcac tcctgaaatc ttcattaatc
     6301 atctctagct agctatatct tcaatcaatt ctaatatgtc ggataaacca tcaacagctt
     6361 cttcaagttg atcaacggat tcttctgctt tttcatatct ttctgatccc tgtagatttt
     6421 taggtatatt ttctttgtac tcttcttctt gaattacttc taactcatcc tttagctcgg
     6481 aaagtcgctc gcttatttct tctaaccttt ttcttcttct ttggttcatc aaaatcacct
     6541 cttcgcataa tctctacgat tttaatatac cacttttgtg tactacattc aatatttttt
     6601 gttatagatt cgtattacaa aaatttcctc taaaaaaata agagcctatt taccatttca
     6661 gtaattaagc tcttatttca tttagttgat aattctatca attaacgtac agcaaccgcc
     6721 attccagtca cattaaatgc tctctcatac gtgcttgcat tgatccattt tgcgccaaat
     6781 acagggtcaa caacatggta tgcaccattt ctgaagccat ccacgagaac tacatgtcca
     6841 ttccatactg cacgacccca aggcatttgt ttgaactctg gcgtagcata gttataagta
     6901 atccaagtta caaccggatt accattgcga atttcattct tgattccatt cgttcctgaa
     6961 ccagtgatat tgacggcatt accgccaacg ttttttgccc atttaactac tgggtcagcc
     7021 atcatgcctt gatacgttcc attaacgtta tgacgccact ctcctgagta accatgataa
     7081 ggattattgt cattagcttt tggcatatag tttacaaaac tggttaagct ttgatttgtg
     7141 gctacacctt tgtagtgtaa accttctaat aaagaagctc cttcgcataa cattgtattt
     7201 ccttgccata caacaggaac gttcaacact ttttcagtgc ttggagcagg tgtaggattt
     7261 ggtacgctgt ttggcattgc ataccagcct gtaccttcaa tcttccaacc ttgtttacta
     7321 attacgttct tttcattttg atctaacgtg tagtgatgcc aattcacgta tttgttatat
     7381 aaacgtgtta caggaactct atttgctccg ttgtaggaat agaacgcaac accttcgtac
     7441 gtccatcctg tgtgtgaaat cttatctctt tctccggcat caactgtgta aaaatgttca
     7501 ccgttatacc gattatacaa acgataaaca ggagtaccac ttgttggagc ttgccatgcg
     7561 acaccttcgt aatgccatcc ataacgtttc agattgtctc tttctccaac gcttctagtg
     7621 tatagatgct ctccgctatt gggattatac atacgataca tggaaataga gctttctgct
     7681 gaaactgaag gtgataataa tactagcccc attgtgaata gaattcctac taaaaatttt
     7741 ttcatttgat tttacccctt ttcatttttt attgtcatta ttgcatgaaa attattcgta
     7801 agattgccaa ttacataaac gattgtttta agatcataat cgttgttg
//
LOCUS       DACCIQ010000106         7783 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCIQ010000106 DACCIQ010000000
VERSION     DACCIQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.109"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(31..1395)
                     /locus_tag="IVM62_002449"
     CDS             complement(31..1395)
                     /locus_tag="IVM62_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6846367.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1392..2951)
                     /locus_tag="IVM62_002450"
     CDS             complement(1392..2951)
                     /locus_tag="IVM62_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846368.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3007..5487)
                     /locus_tag="IVM62_002451"
     CDS             complement(3007..5487)
                     /locus_tag="IVM62_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846369.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5471..5806)
                     /locus_tag="IVM62_002452"
     CDS             complement(5471..5806)
                     /locus_tag="IVM62_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846370.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5820..6854)
                     /locus_tag="IVM62_002453"
     CDS             complement(5820..6854)
                     /locus_tag="IVM62_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6846371.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6944..7624)
                     /locus_tag="IVM62_002454"
     CDS             complement(6944..7624)
                     /locus_tag="IVM62_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6846372.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2229 a         1560 c         1420 g         2574 t
ORIGIN      
        1 tccccacaca ataaaagacc gtgatatttc tcacagtctt ttatctactc tattgctatt
       61 tgctctctta gctagtactt taaaagctaa ctcattgagg tagtgaaaac gagaggtgta
      121 ttcagacaga aatccaaaat cctcaactgc tagctgtgga atcgtagttt tgtgtttcac
      181 gatatccgca agcaggttca aatctgataa taagaattct tggcttatca gctgtccgcc
      241 aattatttct aatgtatccg gatggtagat cagctttgca tttactgttt tgttttcttc
      301 gtcgtattgt ggaaatagat caaattctcc tgaacacgaa ccggtatttt gttccaagaa
      361 tggcgcttca tcttctgtca gccctacagt tcctaaaaag tagtctataa tgactgctgc
      421 tgttatcttt tgtctgaggt cgtaagcggt tggctgacca ctcaaagtta gcgctactgc
      481 tcttgccata tgtacagctc gagtaactaa aggtaaaaag agatgccgtt tatttttgtc
      541 aaaactgact gggacgatat ctccaatcgc atagatgtcc ggatcacttg tctgcagata
      601 ttcattgact atcacagtct catcctcata caaagccact ttttctttta acaatgtcgt
      661 attaggacga gtttgggcag aaaaaatgac catatctgcc tttattgttt gagacagaag
      721 ttgtacggat actactttct cttctgattt tttccaatcg atcaagaatt catttggata
      781 aaaatttatt tgcttagttt gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa
      841 atatcttgat aaaacattcg gcatataatc aactagatga ataatgtatc ctttatttct
      901 caaagcatct gctaattcca atccaatgta accagcaccg aagatcacta cttcttttgc
      961 ttcttttaaa gcggcaagta cttgcttaga ttgcactaga tttttatagg tgtagacatt
     1021 ttcagcttca atttgtttat acaaggtgac aggagaggaa cctgtcgcta cgattaattt
     1081 atcataggtt atggtctcgt tttttccttc tttggatgcg aggatgagct gatgtttgtc
     1141 tggatcaagt tcagttactc tccattcggt gatcagtttt gccccacttt cttgaagagt
     1201 ccgcatgtta tgggcaactt cagctaaact acgtatcttt ccttttaaat aaaggttgat
     1261 tccatttgaa acatagccta gtccatcttt atgatcttca tcgatcagta aaacttctgc
     1321 tttcggttga agttttctta gatttagggc agctgtgatg ccttcatggg aagcaccaat
     1381 gatcacgatt ttcatgtttt attcatcctt aaattcaatt tttctaaata acattctttt
     1441 aaaaattggt tcaaataggg gaaatcataa ttatattcgt tgctagtgaa tacaaaaact
     1501 ttagcggact tctgtgacgg gtactctttc aattggctag tcaaaatgat atcatactgc
     1561 ttttctttct cgtattcgac taaatcatat ttgatcgtat tcagcaattg ttcctggatg
     1621 atttctttca cgatatccgc cattagcctt tcataaggaa gatcataagc aatttgcaga
     1681 cgtaattcta actgcttgat cagcgtccgt aaaatagatg catagcgagc aaataattca
     1741 aagcgggcat tttcctcgtc tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc
     1801 aatctttcac ataaaaccag gaattgttta tcttctactt cagttaaccg ctgttttaat
     1861 ccttcttggc caaaaatatt catccatcct ctaaaatagg caaaatgagc gtgattttgc
     1921 atcactgtca acgcaatctt ttgcgaaatc tcttctggaa aaacataaaa agagaaatat
     1981 tcttctatcg attgtttaaa cgttcgattc aattgagcta ctacgtcgtc tttgtatttt
     2041 tcgcttatca aaaactcctg gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc
     2101 atgaaaagat aaaaaaccaa cgtttcattt tgattccaag gaaaggcata ttgaaagaag
     2161 taacgggtca aaaatgtatg gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca
     2221 ggaagattgc tataatctgg tttggatttt ttcgtacagc gcattcgctt cttcataatg
     2281 gaaagccata tttcaaattt caatcgtcct tcttctgtaa tagcaaaact aaactctttt
     2341 tgcagcagat ctaaaaaagt gtttcctgat aaatcactgt attctttttt gttttcaaga
     2401 tagggacgtc caaaccaaaa taattggaaa taaaaataac gaatttgact ttctggaccg
     2461 gtcagccgcc cgctttttat tttcagatga aactcattta aagaatcatt taattccttc
     2521 actctgcgag acaaagtagc cgaactgata ttaagttcca tgacaagttt ctgccgatca
     2581 aatgagccat tttccaacag ataaagaagt atctgatatt ttgtagaata gcgcaagtaa
     2641 caatgataat aaacatcgac tgctgctata tttccatgtc tgattaataa aatattccct
     2701 tcttggataa caatttccaa ttgtttgata ttttccaagt tagcttgtaa ataaaacaga
     2761 tcttctttca aggtagcccg atcaactgat aagcgggtaa gtacgttatt aacggaaacc
     2821 tctccttttt cctctaataa aagttggaaa agtttgattt gccgttgttc atttttttca
     2881 agcaaactga aaagttccaa gtccactcct tcaagcattt ctgattttaa cagcagaagt
     2941 ttccttctca tccttgaaaa agaggatctg tgacagttat cacagatcca tacaaattct
     3001 aatcacttag ctggttggcc cagctgtcag ttcccatgtg accatgcctt gatacgttgc
     3061 gccaggttga gcattcgtac tgctatttac ctctaagagt aaaccatcgg tatcgggaaa
     3121 gtcaacgaga acctctcctt tagctgttcc atcactctca taaatcaacg tgttttgacc
     3181 ttgctgaatc aattgctttg atccagaatg attgaagtat aattttgttc cgcttaattc
     3241 ttgaccttga tcattcttaa ataaagaagt caatgtacct gtcagttgcc aaggagacgt
     3301 gcttggaagc cgagtatccc gcacatcaaa agaaaaatcg ccttttgatt cataataact
     3361 tgtcttcaac ggtttgggga gatcgttacc aaatgtaaaa tcatctggta cattaactaa
     3421 tgttaattct tgtttgggga caagaattgt tttagcagat gtttggacaa ctgggttagg
     3481 aagcttagca ccatctgcta atgtaatatt gctggaaagt acagctgcct ttgtcagtat
     3541 gctgacggat tgtaaagaag tatctacagt atttaattga tagttgtaag taagatgaat
     3601 ggtatctcct attttgctca atagatctgc tgggacagaa actccttgtt gaatgccggt
     3661 atctgcgata gttgtaccat ttaattttaa ggaaccattg atataagtag tattttcagg
     3721 aagtgagatc gtgatttttt tgtcggttat cgcatcccca gattttgtta acgtgaatgt
     3781 cacattttct tgaatcgtat ctcctgatag agcagacagg ggatctcctg tctcaggttg
     3841 tctgctaagg ttttttagct gactatctgc actcacagat ggaaaattag ccactgttat
     3901 ttgaggaaga gtggtgattc cagcttgttc agactgccat ttgtacgcaa aagacggata
     3961 catgttagta agtacatcgt ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa
     4021 tgcaggtagt gtagaagtag ctgtatttac acccccgtta tttgattgga tcgtttggaa
     4081 aagttgatct gtccatcctg tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg
     4141 gatcgaaccc ttttttagtt cagttgctga ccaattggcc gtgacttggt acgatgcaga
     4201 agaaaggagc tttccccact gactatcggc tgttaagttt gttggtaaaa ccgttccgtc
     4261 tagcttgtac tgctccaata cctttaaagc aacaggagaa agcaatccgc cttgcgcatc
     4321 tttcccactg tacacgatat tggctgtctg attcaatcca cttgtgcttg ctggagtatg
     4381 agtcagtatt gctccttgtg taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa
     4441 gcgactaaat ggcaactgat caaaacgtcc ttcagtcagc cccatcgtca atgtccgact
     4501 attggtagag cctggaaggt tctggttatt tccatccacg tattctgtgg tatcagcaaa
     4561 tgtattatat tttcctgttg aatcacttgg tgagatgtaa ctattccacg tatttatctc
     4621 tgcacttgca gaaaatgcat atgctcctgg attcaagttt ccgccagtta atttaggata
     4681 aaatctacct gcagctggag ctaaaatcga attgaacata cgatcttgga taatattatt
     4741 cgaatcataa aaatcaacag aaaaaacggc ctttttagac atagaaggtt gtaatttacc
     4801 cttggtcaaa attttgtttg gactcgtatc atatttatct ccgttttctg tcatcttcgc
     4861 gacattagct gtaacatcaa attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt
     4921 aataatagca ataatatctg tcaatagagt atttgtatcc cacgacccca tcccaaaacg
     4981 caaggtatgg cttcctgctg ataataaact tccgctagta accttgatgg gggcaccggc
     5041 aatatacaag taggcgttgt tccagtcaat tgcttttaat acatcactgc ttgatacatg
     5101 ttccggcaaa ttgatgatcg ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc
     5161 aagtacattt gttagtttca catggctttc ccaatgaaaa cggaccgtgc cacctttcca
     5221 aggatcattt gaaccatcgt tcaaataagt agcagtcaat ttaggcaatt ggttagcccc
     5281 agttagttgg acaagcttcc cgccattgac tgcactacgt ccaccatttg gaggtacgat
     5341 attttcatac gtgtatggat cataagaaat ggcaggaggc gttgctccaa cagcttctgc
     5401 cgaaggtgaa aagataaata tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa
     5461 aaatgaaaat ttattttgta gcaacatgtt catcctcctt ttcctttctt ctccataaca
     5521 acaaccacgc aagaagccat aattctatac caatgatcaa ataaagaaaa cggatctttt
     5581 gttcattcgt ctccgggaat acgccatgtt gattagattc tttataaatc ggtagctgtg
     5641 tctgctgttt tgatgcgcca ttgactgaac cacttggatg cggaattgga cttagatcat
     5701 aaaatgtaat cgtacctgaa cttttggtat ctgcttggac tggctcttgc cagctgaaga
     5761 acagcaaact tgcaataatc cctcccaata agatcgatgt tttcaacgaa atcacctcat
     5821 catgttgttt ctcctttttt ctttttcttg tggacccaca aaaacagcaa aatcaaccat
     5881 aacactagaa ctgcagcaac caatcctgca taaagccatg ggaaagattg ttcttgtact
     5941 gcaaaaacgg aattttcatt tactttttgt gctttttctt ttttgatcgt aaagttttgt
     6001 gtaaatcgcc attcattact gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg
     6061 gcttttgctt tatcaccagc taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact
     6121 aaaaatgcat tagacgtatt ttcaatataa agctgatttt cataaaagat cttgctcgta
     6181 aatatcaaat tgctgataat cctaggctga ggattttgga aacgaagctg aacagttggc
     6241 tttgcgttga cctcgtccaa atctcctgat aaaagtttca gatccggttc tatcgtctct
     6301 tgactttctt gtaaaacgac tgcgagatta taagcaatac gatttttgat ttgtgctcct
     6361 tctgtagcat tttctgtttc agcttcttct ttaggtgaaa catgaatacc cgctaataca
     6421 cgtcccttaa agctttttgc tgggattttc acttctatag gtacagttgt ttctgcatgt
     6481 gctgatacat tgatttcctt tttcgcaatg agcaaaacgt cacggacatc aaacggcaat
     6541 gtctcatcta acgttgggtt tttttgacta taatcgatcg ttccaccatc actcgttacc
     6601 gccggattga cagagacctc aaatgttcgt tcttcatctg aagaattgct gataattact
     6661 tctagtgtct gattttgttc cggtgccact aataaatcaa aataaccgat tgctttatct
     6721 atttggttct ctggaaatac cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg
     6781 ctgattgacg gaaaaatgaa aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt
     6841 ttactttgat tcattgtcca ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca
     6901 cctatagtat tttcaccgca gtttgttgaa tttatttcat actctatgct actgggccag
     6961 ccacgaggtt ccaagtcaaa tcagccgtga atgttccagc tgctgctttc ggcactttca
     7021 gtgtgatatt tgctggatca taaacagaaa gccatgttcc cagaccttga tcttttgctg
     7081 ctgtaaatat tggtgtcgct gcacttgctt tactcaaccc agttacagtt tgactagttg
     7141 gtccagtgga ttcgttatct gacgtacttt taacagtgga tgttccaaaa tctaattcag
     7201 cgccttgcaa aacttgtgaa ccgtttacaa aatctgaaac cgaaactgtc acgttccacc
     7261 cttgtgcttg gccatctgca cctacacctc gacgatctga tacttggaga taaggtgtat
     7321 cgttttttga agcgtctact tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa
     7381 atggcaattc gggaactaca tccagtgtta aaggacctgt ctggccagtt gctggatttt
     7441 ccggatctgc tggctctact ggttttgaag gatcagtagg gttaagaggc tccacggtat
     7501 ttgttggttc agtaaaggta atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg
     7561 gtgttgccaa gccgcctaga agaagtgttg aaagtaataa tgtcccaatc atgttctttt
     7621 tcataataaa tcactccttt ttcttagtga cctaagtata gtagagacag tatgcaaaaa
     7681 atcctcatga attcatccgc ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa
     7741 aacaataaaa tcgtttacat gaatattctt ccttttttat taa
//
LOCUS       DACCIQ010000107         7682 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCIQ010000107 DACCIQ010000000
VERSION     DACCIQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.110"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            87..2414
                     /locus_tag="IVM62_002455"
     CDS             87..2414
                     /locus_tag="IVM62_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6846373.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2540..2869
                     /locus_tag="IVM62_002456"
     CDS             2540..2869
                     /locus_tag="IVM62_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6846374.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2909..3913
                     /locus_tag="IVM62_002457"
     CDS             2909..3913
                     /locus_tag="IVM62_002457"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6846375.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3910..5339
                     /locus_tag="IVM62_002458"
                     /pseudo
     CDS             3910..5339
                     /locus_tag="IVM62_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5545..6717
                     /locus_tag="IVM62_002459"
     CDS             5545..6717
                     /locus_tag="IVM62_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6846376.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6879..7334)
                     /locus_tag="IVM62_002460"
     CDS             complement(6879..7334)
                     /locus_tag="IVM62_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846377.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2605 a         1443 c         1523 g         2111 t
ORIGIN      
        1 actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt aaaggattat
       61 ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc
      121 aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact
      181 tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta
      241 tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa
      301 atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg
      361 agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag
      421 cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta
      481 ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat
      541 atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt
      601 tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt
      661 ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg
      721 tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt
      781 ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg
      841 gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg
      901 ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca
      961 atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa
     1021 cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag
     1081 cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg
     1141 tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt
     1201 ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag
     1261 acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgacag
     1321 aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa
     1381 tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg
     1441 caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga
     1501 tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca
     1561 gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg
     1621 aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac
     1681 tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct
     1741 atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa
     1801 aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc
     1861 cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata
     1921 tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag
     1981 cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact tataccatct
     2041 ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc
     2101 actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt
     2161 ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag
     2221 gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga
     2281 tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt
     2341 taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag
     2401 gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt
     2461 tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac
     2521 atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga
     2581 cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc
     2641 tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga
     2701 cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac
     2761 aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca
     2821 aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag taaaagaaaa
     2881 atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac
     2941 catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac
     3001 agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc tcatcaccgt
     3061 aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc
     3121 tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac
     3181 atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa aagatcttag
     3241 ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga
     3301 aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca
     3361 tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa
     3421 actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg
     3481 gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga
     3541 tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc
     3601 gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta
     3661 tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa
     3721 taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg
     3781 aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttgttgct
     3841 agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag
     3901 ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg
     3961 ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta tcacctacac
     4021 cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa
     4081 taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca
     4141 acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg
     4201 ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag
     4261 gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg
     4321 aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca tcagaccctg
     4381 acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa agcaatatca
     4441 aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg caattccaga
     4501 tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt gtcgctctct
     4561 atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc atcagtgtca
     4621 atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat ggttatcaat
     4681 taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg caacaaacaa
     4741 ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt gtagtcaatc
     4801 gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt
     4861 tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca gaattttcta
     4921 ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat cttgcctcag
     4981 gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa gaagtgaaat
     5041 tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg gcatactgcc
     5101 ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct gcgtacactg
     5161 aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat aaccaaccta
     5221 aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg gtaggtactg
     5281 ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca gaagcttaaa
     5341 ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg ggagaaaggt
     5401 tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag gaggaactat
     5461 tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc tcttcatata
     5521 catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact ggacatcttc
     5581 atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt agaagattca
     5641 ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc atcgaataag
     5701 aattcagagg aaataaaaaa acagcaggaa aaaatgacaa agaaaaacca acagttggca
     5761 gagcaaaacg tatcaccagg gataggcagt ttcaaccaag cagttgatgc aaaagcatta
     5821 aaagatttac ctagtaatgc cttttttatg gaacatatgc ttggagtcat cgaaatccca
     5881 aaaatcaatg tcagcttacc tatattcgat cagacaacgg aaatattttt gcaaaaagga
     5941 acgtcattat tggaagaaag ttcttatccg actggcggga aaaacaccca tgccgttctt
     6001 tctgggcatc gtggactccc agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa
     6061 ggagaccagt ttttcatcca gatcaatgga aagacattgg cgtatcaagt agaaaaaatc
     6121 caagtggtct tacctgatga agtagattct ttgggtatcc aaaaaggcag agatctcgtg
     6181 actttgctta cctgtactcc ttatatggtc aatacacatc gactgcttgt gacggggcat
     6241 cgtattcctt atcaagcaaa agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa
     6301 aaatggaaat tttttatttg gtttatcggt atactacttg gatcaatcgg cttagtttgg
     6361 ctactcatcg cttatttaga ttctttggcc atcgctaagc gaaactatcc tttatctttt
     6421 tatgtaaaga acacaaacgg acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc
     6481 aacggcaaac actatattac tagagaaaaa gttccatttg tgaaagcttc tgatgaatac
     6541 ggactagtga gattttctga tttaaaaggc aggaactatc gcttgcaaca tgaggagctc
     6601 ctgcttaaaa tccatgtaaa acataaacat tcgaaacagt tttcaatgaa attgaaaaaa
     6661 ggaagatata aactgcgaaa agaaaaagag gtgtactatt tgattgagaa agaatgaatg
     6721 aagatgtcca aaaaagcaga gggaacagac taaatgaaaa aagagagaag ctgacgaaat
     6781 ggacgtgtaa actgctattt ttggtttact atctggaacg ccagcttcta tttttttatg
     6841 tttcagaaga attttattat tatagtataa cttttgaatt agtttccata acatttggag
     6901 caaggggtta aaccacgagc ttgagcttcg cttaaagtgg caggggtata cgtaccattt
     6961 ccacatttat gtgtatggta ttttgaaccg gtcggtgtga catagacagt ttgttcatta
     7021 ttttgctcag gagctgcggc ttgtgcctgc tgagaggcct gttcagctgc tgcttgctgt
     7081 cgcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt
     7141 cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa
     7201 tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct
     7261 gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat
     7321 tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt ctcttcttct
     7381 tttttctttt caatttgttc ttgttctttc gatgaatctg ccgtactttc ggagatcgtg
     7441 gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt agtagcacca
     7501 cacccactca acaaacttcc tgcgaccaaa aacaatgcaa ctaatttttt cactaaattt
     7561 cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt ttacttactt
     7621 gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc cgttttgata
     7681 ca
//
LOCUS       DACCIQ010000108         7599 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCIQ010000108 DACCIQ010000000
VERSION     DACCIQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.111"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(137..427)
                     /locus_tag="IVM62_002461"
     CDS             complement(137..427)
                     /locus_tag="IVM62_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846378.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IVM62_002462"
     CDS             complement(545..805)
                     /locus_tag="IVM62_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846379.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IVM62_002463"
     CDS             complement(942..1355)
                     /locus_tag="IVM62_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP6846380.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IVM62_002464"
     CDS             complement(1811..2296)
                     /locus_tag="IVM62_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846381.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="IVM62_002465"
     CDS             complement(2435..2650)
                     /locus_tag="IVM62_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP6846382.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IVM62_002466"
     CDS             2874..3674
                     /locus_tag="IVM62_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6846383.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IVM62_002467"
     CDS             3693..5561
                     /locus_tag="IVM62_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6846384.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IVM62_002468"
     CDS             5554..6045
                     /locus_tag="IVM62_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP6846385.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IVM62_002469"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IVM62_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP6846386.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="IVM62_002470"
     CDS             6605..>7599
                     /locus_tag="IVM62_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP6846387.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACCIQ010000109         7397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCIQ010000109 DACCIQ010000000
VERSION     DACCIQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.112"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..142)
                     /locus_tag="IVM62_002471"
     CDS             complement(<1..142)
                     /locus_tag="IVM62_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846388.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IVM62_002472"
     CDS             complement(183..437)
                     /locus_tag="IVM62_002472"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6846389.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IVM62_002473"
     CDS             complement(548..802)
                     /locus_tag="IVM62_002473"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846390.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IVM62_002474"
     CDS             complement(995..1270)
                     /locus_tag="IVM62_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP6846391.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IVM62_002475"
     CDS             complement(1242..2195)
                     /locus_tag="IVM62_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6846392.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IVM62_002476"
     CDS             2807..4300
                     /locus_tag="IVM62_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP6846393.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IVM62_002477"
     CDS             4435..4731
                     /locus_tag="IVM62_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP6846394.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IVM62_002478"
     CDS             complement(4755..5714)
                     /locus_tag="IVM62_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6846395.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IVM62_002479"
     CDS             5897..6748
                     /locus_tag="IVM62_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846396.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IVM62_002480"
     CDS             6769..6981
                     /locus_tag="IVM62_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846397.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACCIQ010000110         7298 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCIQ010000110 DACCIQ010000000
VERSION     DACCIQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.113"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..631
                     /locus_tag="IVM62_002481"
     CDS             <1..631
                     /locus_tag="IVM62_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846398.1"
                     /translation="TKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDL
                     LVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPEN
                     SQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKE
                     KRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            814..3489
                     /locus_tag="IVM62_002482"
     CDS             814..3489
                     /locus_tag="IVM62_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP6846399.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3671..3910
                     /locus_tag="IVM62_002483"
     CDS             3671..3910
                     /locus_tag="IVM62_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846400.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3926..5299
                     /locus_tag="IVM62_002484"
     CDS             3926..5299
                     /locus_tag="IVM62_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6846401.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5303..6937
                     /locus_tag="IVM62_002485"
     CDS             5303..6937
                     /locus_tag="IVM62_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6846402.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7100..>7298
                     /locus_tag="IVM62_002486"
     CDS             7100..>7298
                     /locus_tag="IVM62_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846403.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2603 a         1137 c         1450 g         2108 t
ORIGIN      
        1 gactaaattt gcaaactcat tctggttttt aaaaaatatt aataaaacag tggaaaagaa
       61 tggagtttat aaaggtgatt ttcattttca aaatgctgag actattgaac gagaacatca
      121 ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa agtcaaacga
      181 aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta tctatgagtt
      241 aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt tagataatcc
      301 agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga cactaagaat
      361 tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa ttgaaagaga
      421 ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg aagataaaga
      481 gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt taaaatgtag
      541 aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga aaatatataa
      601 agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta tcgtaaaaga
      661 aaaaagttgt gatttccaaa atcacaactt ttttcttttg tcaagatttt gagtgggata
      721 tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg tagtcataca
      781 aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac ttcaattcag
      841 aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag aactttttct
      901 acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca agcaattatt
      961 aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta taatcctatt
     1021 aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat tgaaaaaatt
     1081 cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac agatagcgaa
     1141 aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga aaagagaata
     1201 gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc cataaatgaa
     1261 caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt acttcaccga
     1321 agatttggag tagaagatga agttgatatt gatacttctg tttataatcc ttcaagatta
     1381 tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg tccgcatcgt
     1441 ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt taaaattctt
     1501 gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag ccatccatct
     1561 tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc tcactatcct
     1621 gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact tatatttgac
     1681 gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc tgttacggct
     1741 gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga tattcaagac
     1801 tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc attagaatcc
     1861 aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt tttggtgaaa
     1921 gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg caaaaatatc
     1981 gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg ggatgaaata
     2041 gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa acatggctta
     2101 atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca acgggagaat
     2161 gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga agcaatgatt
     2221 tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa aaactcatgc
     2281 taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca taagtatgtt
     2341 ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt gttaggctat
     2401 ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca gggcaagagc
     2461 acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa tccaactacg
     2521 gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc aaacaatcag
     2581 ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa ctttagctcg
     2641 ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa ttgtcagctt
     2701 atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg tttggaaaat
     2761 tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc tgatatcaag
     2821 tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa tacttttggt
     2881 tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa aattgaagca
     2941 acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc tttgaagcaa
     3001 cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca agatatactg
     3061 ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta cgaacagtta
     3121 attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa aacattgttg
     3181 gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc cagtacgatt
     3241 tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt tcctatcccg
     3301 tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct ttttaggcaa
     3361 ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg tattcacatg
     3421 aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa atcgtatttt
     3481 cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat ggaaatattt
     3541 cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca aacccaaacg
     3601 gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca tgaaagaaag
     3661 gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc tgtatattca
     3721 agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt tcgaaataag
     3781 aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg aaggatattt
     3841 agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat ggtatttagt
     3901 tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa cgtctgttta
     3961 tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt atgttttaga
     4021 gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg aaaaagcgga
     4081 gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag agggggtgat
     4141 ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag aatttattga
     4201 gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc ctgcttcttt
     4261 ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg cagaacaaca
     4321 acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc aacaacggca
     4381 atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa tccaacaaga
     4441 agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca aaaaatatgg
     4501 ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg aacgtccttt
     4561 tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg aaatcaaagg
     4621 aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag caaccttttt
     4681 acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt atctgtactt
     4741 attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga agtatcaatg
     4801 gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa gctgccaagg
     4861 aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac aggctttttt
     4921 tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat tagccgaaaa
     4981 gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac aatggattaa
     5041 ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc aagctcttca
     5101 aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa aagtgaaaga
     5161 atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg tttgtcggat
     5221 tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga cacaaaatat
     5281 tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg cattgtatat
     5341 tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac aagaacaaaa
     5401 tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg ccgatgaagg
     5461 aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga acgatgctcg
     5521 gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac gcaatatgtt
     5581 agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac attcgatctc
     5641 ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc ttgggagttt
     5701 cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc aaagcttagt
     5761 gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg gtattgacac
     5821 gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc gtctgatcta
     5881 tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga atcgtcaagg
     5941 atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata tcttacgcaa
     6001 taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa ccaaacgacg
     6061 gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga ttatttcgga
     6121 agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac ccaaatggaa
     6181 ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg gcgcaccgat
     6241 ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga ttcgatatta
     6301 ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt cgattcgtgc
     6361 cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc taccagaagt
     6421 tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc aaccattgga
     6481 acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa ttgaaaaatg
     6541 ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc gcttagacga
     6601 attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag ggatctttca
     6661 acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactagtt tggatcgttt
     6721 tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta tagagaaaat
     6781 aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca cgcatgccgt
     6841 ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg atacggctat
     6901 ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc aaacaacgtt
     6961 agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt tagggaggtc
     7021 cttatcataa atagggatat ttattaatga atatttgaaa tataattgta tataagaatt
     7081 aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg aaaaatacac
     7141 tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag gtaaaaagac
     7201 cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg attttccgaa
     7261 ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DACCIQ010000111         6924 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCIQ010000111 DACCIQ010000000
VERSION     DACCIQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.114"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..183
                     /locus_tag="IVM62_002487"
     CDS             <1..183
                     /locus_tag="IVM62_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6846404.1"
                     /translation="HNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDL
                     HDEWIYSSRKYINFDK"
     gene            complement(337..867)
                     /locus_tag="IVM62_002488"
                     /pseudo
     CDS             complement(337..867)
                     /locus_tag="IVM62_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            939..2231
                     /locus_tag="IVM62_002489"
     CDS             939..2231
                     /locus_tag="IVM62_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6846405.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2443..3285)
                     /locus_tag="IVM62_002490"
     CDS             complement(2443..3285)
                     /locus_tag="IVM62_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846406.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3322..4788)
                     /locus_tag="IVM62_002491"
     CDS             complement(3322..4788)
                     /locus_tag="IVM62_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6846407.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4844..6688)
                     /locus_tag="IVM62_002492"
     CDS             complement(4844..6688)
                     /locus_tag="IVM62_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6846408.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
BASE COUNT         2128 a         1448 c         1157 g         2191 t
ORIGIN      
        1 cacaatcgac taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga
       61 gaaaagatta ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt
      121 cttatggacc tgcatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag
      181 tagaaatggt aaaaacattg tatagcattt tacacaggag tctggacttg actaacattt
      241 ccatttcatt tacctctttt tcgtttaatt atactaaagt tctatccaag cgacaagctg
      301 tgtataataa aaatataccc gtggtgctag ttaattttaa taccctaaag aatatctcaa
      361 agtcccttca aaatgttgct gtgctcggct taaaagaata ctgtacgata ataatatgct
      421 ttcaaactta ctttctagcc cttgaataga acgtgatttg aaattttgaa aacctagtct
      481 ttcgagagaa gaaaaaacag tttctactgc ttttctttgt ctagtaagta atcgatggtc
      541 tatcttccct agtttatcca tgttttttcg tgacggaact aagactgtaa tgcccttaag
      601 ggcaagcttt tcatggattt tatggctgac atagccttta tctccaagga tatttggaag
      661 gtttgcttct tccgataaag tctctaaaac tttcacatca tgaacactag gatccgtgat
      721 tgaataagta actggaaagc ctgttttagt gacgataata tgaattttaa gaccataaaa
      781 ataagcgttt ttcgttgagt tatagcccct acagctctat actttttgat gttggtggat
      841 gacaccagca tcttttttat tttccaaaac atatagaaaa gacaccttga aatcctttag
      901 aattaatttg gcgaaccaac ccaaaaggag gaatcaagat gtcttacact caccttatca
      961 aagaaacatt agatattcta gacttatcag ttacttttaa cgaaaattgt ctcacaaaag
     1021 aaaaatacaa agggcaaatc tgtcatattt atcgtggaaa cttgatttat acagcccaag
     1081 aatgcatcca ctgtaaacac cagattgctt cagatattgt gcgttggggt acaacaaccg
     1141 tccggttgtt aatgaacgat gtttctgaat gccgtaccta cctcgaattg aaaaagcaac
     1201 gcttcaaatg caaagcttgt cagcggacct ttgtcgcaga tacttctgtt gcgaaaaaac
     1261 actgtttcat cagtgagaaa gtccgctggt ctgtcgtgac tcgtctgaaa aaaaatacct
     1321 caatgacaga gatcgccgct cagaaaaatc tatcggtctc ttctgtttac cgtattatga
     1381 agcgatttta tcgtccgttg aatccttttc ggacaccgtt acctaaggta ctttgttttg
     1441 atgaattcaa atcagttcgg ggtgtttcag gtgctatgag tttcatcatg atggatggac
     1501 aaacacagcg cttgttggac attgtcgaga accgacaact gcctttttta aaacgttatt
     1561 ttagtcattt ttcacgagaa attcgtgaag cggtcgaatg gatcgtgatt gatatgtacg
     1621 ctccttatgt atcgctcgtt aaaaagctgt ttccaaaagc acagctgatt attgaccggt
     1681 ttcacatcgt ccaacatatt ggtcgtacct ttcgcaatca tcgaatcaaa gaaacgaacc
     1741 aactattaaa aagcaaagaa caaaaacact atcaattagg taagcaatta aaaagatact
     1801 ggaaacttct tcaaaaagat gaacgcaagc ttgattatac tcgacgcctt tggcgacctg
     1861 gcttcaaagc tcatttaaca gagaccgata ttgtggatcg cttactgaaa gggagccctg
     1921 ctttgcgtgt aggctatcag ttatatcaag actttcttta cgctgtaaag gaacgagact
     1981 acgtatcgtt tgaagaacta cttaccaata atatcatgct tcccgaaggc tatcagacga
     2041 ccttgagaac gttccagaaa tttctgccac aaatcaaaaa tgctttacaa caaagttatt
     2101 caaatggccc acttgaatgt ctgaacaacc atatcaaagt gttaaaacga aatgcatatg
     2161 gctttcgtag cttttataat ttcaaattgc gcatcatgat tcgtcatggc aacgcattga
     2221 ttttcaacta aaaaagatca agaggaactg atccacttga tctccaatta ttctaagatt
     2281 tctctttacc aacatcattt gacaaagagc cgagattttt tattatctta cgttaatcct
     2341 ttttaaatgt ctcttataga taacaaacag ctggaaatca ccttgttttt actataatcc
     2401 atctctaaag attttaaaag catcaatagt attactgagc gctcacccat tagcatgttt
     2461 catcaaccga ttgatgtgga cacctaggta tacaacttca tcgttcggaa ttgttttatg
     2521 ataaactttt tcaatgtagt ctttgatctt agctgcgctt tccatggcag acgggtataa
     2581 gatccacatt tgattgtaga attcttcctc accatcatcc aacaaagcat cagaataata
     2641 tctttctaca aagaaacgta catgtgtaat gaagcgggca taatggatcg aattagtatc
     2701 gatatctttc tgcaacgaat accggaccaa attgacgatc ccgccgatca tttttgcata
     2761 cttgaatcca gcgccgctat caggatcttc cgattgtgca ttgatcaaat ggaaagcaat
     2821 gtttgaggct tcctctttag gaagatcaat tccatattgc tcgttcaaaa gggtgagtgc
     2881 gtacttccca atttgaaatt ctttgggata gtagattttt atttcccagt acaaacggtt
     2941 tgcaatattt accccttttt gtgcgcgttc aactgcaaaa tgcaggtggt ctgttaaggt
     3001 taaatagact ccaggattca gttttttatc taatcctttt tctgcttcaa aaacgatagt
     3061 acgagtgatt tcaaaaaatt caataggtat ttcttcaact aactctaata gttgtgaaga
     3121 tttcttttct tcaaaaggca gaaatatttt atctacttta tctcctgata tttcatcccc
     3181 agctttctgt ccaaagccta tccctttacc taatgcaatc atttctttgc cattttcttc
     3241 aaccagtacc acacttgaat tcaatacttt ttttattgaa atcactcatt atccccctat
     3301 cgctgcttaa aaagatcgag actactcgaa taaggatgca ccgtttgttt cgatcacttc
     3361 tttgtaccag ttgaacgaat cctttttcaa gcggttatat gtcccatttc cttcatcgtc
     3421 tgcatccaca taaataaatc catatcgttt agacatttga gaagttcctg cactaactaa
     3481 atcaattggt ccccagctag tataagctat caaatctaca ccttcatcga ttgcttcttt
     3541 catttgttgg aagtgtgctt taaagtagtc gatgcggtac ggatcatgga ttttaccatc
     3601 tactacctga tcgactgctc ccataccatt ttccacaatc atcaacggta cttggtaacg
     3661 atcataaagt tcgatcaacg aaattttcaa accttttgga tcgatttgcc agccccaatc
     3721 tgttgaaggc aaatgaggat tcttaacccc taaaacagta tttcccggtg tgcgttctgc
     3781 gtttggatca gctgattctg ccatggacat gtaatagctg aaagaaacaa aatctacggt
     3841 atgtttcttt aaaatttctc gatcttcatc ttccatttta atctgaattc cccgattttc
     3901 caaatccttt tcgattagag caggatattc tcccttaact tgaacatctg cataaaaatt
     3961 attttctaag ttctttttca aggtcagctc aacgtcttct ggtgcgcaag ttaatggata
     4021 agtcatcaat ttcgttaaca tacaaccaac ttggcttcct ggaatttttt catgagcatc
     4081 ttttgtcact aacgcactcg caacaaattg atgatgcaat gcttgataga tatcttgagc
     4141 ttcttgacct tctgcgcttt tctcttggat aattccagct gttgtaaatg gatgacggtg
     4201 gatgctgtct atctcattaa atgtcagcca atattttacg tatttgccga agcgatcaaa
     4261 acaaacgtta gcgaagcgaa caaaatcatc aattactcga cgctccaccc agccattata
     4321 tttaactgct aaagctaatg gcatttcata atgagatagt gtcacaatcg gctcaattcc
     4381 taaacgttgc atttctttaa agacgttttc ataaaatgca accccttttt cattcggttc
     4441 cagctcttct cctgtaggga acaagcgagt ccaagcaata gagaggcgca aggttttaaa
     4501 gcccatttcg gcaaataaag ctaaatcttc tttatagtga tgataaaaat caacgcccct
     4561 tctttttggg taccatttat cagatttgtc ttcactagct tcttttatag actgcagggt
     4621 gacatgcata tgagcagcaa aatctttgtt gtctaaattt ggtttataag tagccatatc
     4681 cgctactgaa agacctttac cgtctacatt ccaagcacct tctacttgat ttgctgctat
     4741 cgcgccgccc cataaaaatc cttttggaaa acttgttgtg tttgtcatta ttctattctc
     4801 ctctattcct tcattcaata tatttttttt cactatcttg tgtttaagct atcggactga
     4861 tagctaagag atcactatca ttggttacca atttttctag gttaaatgaa cggatagtgt
     4921 aattatcctt attggttacc acacagataa ctgtggggtc gaatcctgct tctttgatta
     4981 aatcaatatc aaatgcaatc agcttttgtc ctttggctac tttttcacca gaacgaacaa
     5041 atgcctcaaa cccttttcct tctaactgaa cagtatcaat accaatatga aaaagaagtt
     5101 ctgcaccatt agccgttttt aaaccaatag catgcttcgt ttcaaatacc attgtgattt
     5161 ctccatcttc aggagctact aatatccctt cttcaggaat gattgctact ccatcaccta
     5221 ccataccact agaaaaaaca gtgtctttta cttctgatag tgagattact tttccattca
     5281 cgggacttga aagaacagca tctgaaacaa cggccgtttc catcttagtt tcaacttcat
     5341 tgacaacttc tttatcttta taaagaacca acgctgcagc aaaagcaacc acaaatgaaa
     5401 tccccgatac tgcaaatgcc caaaccagat tcattgggtc agctttgtca gcgaacatcg
     5461 tgatacttgc aatcccagga cccactaaag caaaggcttg aagtgcaacg attcctgcaa
     5521 atgctccccc gatcaggcta gaaaacataa cggcatataa aacacgtttg tgcagaatcg
     5581 ttaccccgta aagagctggt tcagtaattc cgaataatgc agatataccc gcagaaacag
     5641 ccgtagattt caattttttg tcttttgttt tgatactgac agcaaagcat gcgccagctt
     5701 ctgaaatatt gtgtgccaat gaagccggga gataaagaag ttctgcaccc aattcactca
     5761 tggatgaaac agcgtaaggg atcattgctt tatgcatacc cgttacaacc attaatggca
     5821 gtacagctgc taagactccg gttgcaaccc atccaaattg attgtacaaa gacacaatga
     5881 tagaagagaa agctgttcct acattataac ctaatggtcc tagaagcaac aaagcaatag
     5941 gaactgtaat caataagctc atcataggta caaagaaaat acgaattggt tttggtgaaa
     6001 cttttgtaaa ccatttttca agttgagcat aaaataaaac agttaagatg gctgggaata
     6061 cttgagatgc atacgctata ttatccaatc caaaagataa gaatgtagca ccttccgcca
     6121 acatagctgt catttcagga aggatcaatg caccgacagc agatactgca accaattgat
     6181 tgactttcaa tttattagcc gtagtcattg ccaccagaat aggcaagaaa taaagcggtg
     6241 ccccgccgac caaatttaaa atctgatagg tttgagattg gtcactaatc agccctaaga
     6301 tagaagctag tagcaacaag gacttcaata ctcctccacc tgcaatcgca ggaattaaag
     6361 gttggaaaat agaaataata aaatctaata gaactgaacc taatttttgt ttttgttttg
     6421 gtgcttcttc agtatttgaa acattgtcac ctaataacgc tttcacttca tcatacactt
     6481 caacaacatc gtttccaatg attacttggc attgcacatt ttggcgaacg cccataacgc
     6541 catccacttt ttctaactgg ctcatattta ccttgctgtt gtcttttaac gtaaaacgta
     6601 aacgagttga gcaatgctcc aaatgggata tgttttcttc cccaccgatg tactgcaata
     6661 cttcttctgc tgtttgtttg taattcatgg tgtttccctc ttttcttttt tgagtgatga
     6721 acttttgagt cgaagtggcc atcctcaaca caaaaaacag gcagaaacta aataaattga
     6781 caacaaggca aacgtgtatt gaaacgcata cattgttatt caacttattt aggtcctgcc
     6841 tgcattacca gtcacatgcc tgtttaagtt atattcaatt taacatatta gaatgtaaac
     6901 gtaaataata aaatgatttt ttaa
//
LOCUS       DACCIQ010000112         6875 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCIQ010000112 DACCIQ010000000
VERSION     DACCIQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.115"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..343
                     /locus_tag="IVM62_002493"
     CDS             <1..343
                     /locus_tag="IVM62_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846409.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTV"
     gene            complement(1061..4210)
                     /locus_tag="IVM62_002494"
     CDS             complement(1061..4210)
                     /locus_tag="IVM62_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6846410.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(4223..5422)
                     /locus_tag="IVM62_002495"
     CDS             complement(4223..5422)
                     /locus_tag="IVM62_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6846411.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5412..>6875)
                     /locus_tag="IVM62_002496"
     CDS             complement(5412..>6875)
                     /locus_tag="IVM62_002496"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6846412.1"
                     /translation="QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDIL
                     GYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTD
                     EQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSD
                     MMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAK
                     MNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRF
                     NRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSI
                     YAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKI
                     LETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQ
                     EQQVLEKELLEAISSLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1927 a         1404 c         1159 g         2385 t
ORIGIN      
        1 catcacgaga catgtttggg gagatctgat ggatgaagtg gaacatcttc gcctaacaga
       61 tctgaataaa tcaatttaca aaaaacggaa acaaacgatt gaacgaatat ttgcggatgc
      121 aaaagaaaag catgggatgc gttggaccaa gtatcgaggc ttggaaaaag tcgcgacgca
      181 tacgatgcta gtgtttgctg caatgaattt aaagaaatta gccacctggc tatggaaagg
      241 caaggagcct ttattttttt gttcaaaaat tcgaaacgaa gtagataaaa agctgttcca
      301 agctcgagtt acgagtttgg aacagctttt gtctacagtc tgaggactaa tacaggtagt
      361 cctctttttt gttattttga tattctaaat cattttttat tggattattc ccttgttcga
      421 tatagaccca aattgatcta tcaataatta tcaaactgag tcatatagag acaaaaattt
      481 caaaaatgac ctgttaaatg gtcatcgaat atcatttaaa atcaatacga cggagtcaga
      541 cacggagtca aaacaagccc gctgagagta aaaaatatat acagtcccat aggtaaagtg
      601 ccacaggact gtatattttt ttatttgaga ttacctgaaa tgattccatt tactttttat
      661 ctcatttttt cctactagtt agtatcaaat actctcctat tttctcaata aactattttg
      721 ggttacatgg tatctgaaac agctaaaacc ttgtcattac agcaaaaaat cactttatct
      781 ccgtttcatt tcagatacat ttcacttaag cggattccaa aaaaacaaaa gaaaaaggaa
      841 cccctagtta gcctatgctt tttattggtt cctctcaaaa cacggtcata taacaacggg
      901 gtggggtaaa tgaatgaaaa aggtggagga aacattattc aatagctgtc ttaaatactt
      961 cgttataaaa tgtgaacaaa taaaagacat ctatccctat tttctacact tccactttac
     1021 taccatcttt gaatcgtaca attttcttaa ctcttctcgt ttacctcaat tcattttcaa
     1081 gtggaatcag ctcatcatct aacattttac gccatgctcg cttcatttct tgtgggtatt
     1141 taaatggctt cgcttctgga tggttggctt tatatccttc gtaatcctta ctgttgataa
     1201 ttgctttcat attaggaatt ggatcttcac ctggcatata ttgcagatta gaagcatgta
     1261 attctttctc agaaacaaac cactcattgg aaaagacatg aatcaaatca tcactcgctt
     1321 gtgtaaaata gcgacgttta atgacaaaga tatcttcatt actatctaaa gtccccacat
     1381 caaacgcatt taaaatttgt cggtaaactt ctttgactcc ttctgtttta ttcaatttag
     1441 ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt cgccgcataa gttcctagta
     1501 attgatcaat ataagtagaa tcaatatcat acaacttcgt ggtattttca ctataaaatt
     1561 cgatcgtgga aaaatcttct ccctcttttt ctggttcttc ctctattaat gaacccttca
     1621 cggtctcgta cacgccaatt tgttcttcca aaagttgaat ttgattgttc aaggttgaag
     1681 atttcaccat gtcatcattg taatcgtcat aagtcactaa cgattcataa gcacgattca
     1741 attcttggaa tgctttgacg tactcgatac ggatttcaag tggcgtatgt tcatcaatgg
     1801 attccatgga tgaaacctct tttaacttac catatgcttg cttgaatctt tttttggatt
     1861 cctgataact tggataaatc aaacccgatt cttcttttgc ttctgaatac agttttgtcg
     1921 catcctcgac attcttctcc attgtggctg gctttcggaa agtaacaatt agtccttttg
     1981 ttttttctgg atacgtacgg ttcgtacgtg aaaaagcttg gatcaatcct gcatactcta
     2041 gattacgatc cacaaatagg gtttgaatcg taggtgcgtc aaaacctgtc aatagacgat
     2101 ccactacgat cactaaatca atttgtttcc caaattgttt gaattccgct ctttttcgtg
     2161 ccaaacgatt attgatgtct ccgttataac gctcaatgtc tgctaatgac caggaagtat
     2221 cataatactg attatattcc tcaataattt tttgcatttc ttcttgttgg gcacttgaat
     2281 ctcggctatt ttcttctaat gaataggtaa tagcgattcg tggaaaatca ggatcctcca
     2341 tcgttcgtcc ttcacgaatg agctgattgg gaaactcttt tgttaaccaa tcaggctctt
     2401 tcgtcatctc tttgatggcc cgataatact ttttggccat gtcaatggaa ctcgtggtta
     2461 agattgcaga tttcgttggc ctgccatttc gaaaatcaaa ttttgtatag gcattatctg
     2521 gacggaacat tttatgaatg actttttgga tatgttcatc tgcttcaaaa acagctgggt
     2581 ccaaataact ttcttttttg acaggttcca tttgatcaat catacgattg atctgttctg
     2641 atgaatactc tgcatatgtt tctacttctt ttaatttccg atagatttta ttctctagtg
     2701 aagtcggctc cacggtgttt tcatgctcta cttgaaatcc taagacagag ccatcttcta
     2761 gcgcattctt aatggtatag gtatgcaaga cctctccata ctgatcatgg gttgtacgtg
     2821 ccaattgccc tttggcttgt ttccgattct caggaaaaat cggtgtacct gtaaagccaa
     2881 accaagtgga tttcggaaac atttttttga tttcttccat attttcagca cttaacgctc
     2941 gatgacactc atctactaca aacacgatat gttggcccat taatttttgg aagcgattgg
     3001 ttcctttttt ctcttcttgt ttctttgcat atttcaacgc cgcatctagt ttttgacggg
     3061 ttgtaatgat tacaacattt gcattggcat ctgccaacag ggtttcactt aattctttgg
     3121 cacttcccgt tccaacgatc aagctattcg ctttggcgtt cccggaagaa atcccagtat
     3181 taaattccga agcaaatttt gtaaactctg tggtggtttg attatctaaa tctttgcggt
     3241 ccactaacat gatcgttcga tcgatccccg attttcgtgc taaaagctta gtagatacaa
     3301 aactagtcaa cgtctttcct gaaccagtcg catgccacac ataccctgat tgatgtttat
     3361 ttgcggcaat aaacaaggct tgaatcgcat gtacttgata aggatgtaac accatgaggg
     3421 ttttattatc ttgatcttca ctgacgattg tataatccgc aatcaaacga tgagcatccg
     3481 ggatatttaa cacttgttta caaaactcat ataagttttc tactttttca ttgtctttcg
     3541 ttcgccaact aaataaaaac ttagggtgca agtctttcgg taaggcattt gcaaaatacc
     3601 gagtggtttg ttcattggaa accacaaaca actgtaaggt cgaaaagata ttgttccgaa
     3661 acataccttc ttctgcatat tttttgattt ggttaaatgc ctgataaaca ccatcttttg
     3721 cagttacttg tttcagctcg atttggacaa tcggtaatcc attgattaat aaggtgacgt
     3781 caaaacgtcg atcccgtgct tctatgttac ttccacgttt tgcaatttga tggaccactt
     3841 cataagtaga gattcctcca ccaatgtctt gattggaata aaggataagt gagacagaac
     3901 ccaattgcga atcttcccgt tcaatcgtga tccgagccat cccattttca ccttttaacc
     3961 atttcgcagc atcaaatggg gttttcgttc gcagcagtaa ttccgttttg atcgtttcaa
     4021 attctttatc ggtcaaagga aaatcattca gttctgcttg attatttgag ataatttttt
     4081 ggcgtaaatt gacccaaagg tcttcttctg attttagatc cggacgatac gtccactgat
     4141 tatgcccttc tccaagtaca cgaatcaact gttcttcgac ttctgcttcc tcacggtgac
     4201 taattttcat tcttcttccc tcctagacaa acatcttttg taagaagctt tttttcgttt
     4261 cttttagtaa atctaactta cgttgatgaa gagcgatcgt atcatctagc tgtttgaaga
     4321 aagtaccgat tttttgttgt tcatccttta caggaacctt aacactgata ttctcaatat
     4381 cagaactgtt ataagaaggc aatgccccta cttgggagta tcttggcaaa tttatagttt
     4441 caaacaatat tttaccaaaa tagatatccc aagtattatt aaataaataa gccatggtat
     4501 tgttatctat taagaaaggg cttgtgacca aacgttttct attaagcatt attgctgcgc
     4561 caactttagc aaatataatc gctggcacat cttttattgg cttccatttt ttacgctcaa
     4621 tttgctcatt agatacataa tgatttgcat ttctcatttc atactcatta ccaatgttat
     4681 tcatgtcgga aactttaaaa aatggagtac cttcagaacc accttgctcg gcatcaggaa
     4741 atccaattcc cgattgagtt ttacctaatt ctcccaactt acgctcttcc caatcttccg
     4801 taaaaccagg aaaccggatt tctggcactt ttgctccgtt ttcagggaac attttttgta
     4861 agaatccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatagtgt
     4921 cgtcgagttt tttgaagaat atgccgattt tttgttgttc ttttaaatca ttgggaatat
     4981 agaatactgt atctgaaact tttttccagt ctgaacgagg catttttgtt ccagatgtat
     5041 catttgcagc cttttgatat ctatcagctt gaataagaga ataaataaag tccgagtcgc
     5101 tcttcttagc tctaaaaacc cagaaatccc caatcgctac gcctgtaaaa gttgccttga
     5161 gccaattttt taaatatgga cgtaactttc catatagaat atcatttggt aaaaattcaa
     5221 tcccttttct attatcaaat ttctggctca catctttatt cagtctacct tctcctgcca
     5281 cgatatcttc aaattcaact tttggaagtc tactggagtt tgtactttta ttaagtctaa
     5341 caactaaatc aattaactta cgctcttccc aatcttccgt aaatcctggg aaacggattt
     5401 ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa ccacatttcg
     5461 ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc
     5521 tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct
     5581 tcttcgaaag tatctacata gcgtgggata tttaagttat agtcattttc tttgatttca
     5641 tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta ggtttcgagg
     5701 attttttgaa tgttttcttc cgatagttta ttttgatttt tccctttaac aaattcacga
     5761 ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaacac aatcaccgtc
     5821 gttggaatcg atgtcccaaa gaacaagttc gcaggcatac cgatcacagc ataaatactg
     5881 ccatcttcta gtaatttttg acggatgacc ccttctgcag cgccacggaa taaaactccg
     5941 tgtggtaaga cgatggccat ggtacctgtt tccttcaaat gatagaaccc atgaagaaga
     6001 aacgcaaaat ctgctttgga ttttggcgct aatttcccat aacgattaaa ccgagaatca
     6061 tctaagaaag tcgtatctgc tgaccaatta gccgaataag gtgggttcat tactactgca
     6121 tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc attgcggaga
     6181 ttcatttctt cggcgtcgac accatgtaaa atcaagttca tttttgctag attgtatgtc
     6241 gttgtattta gttcttgccc atgatattta acgttatctg ggtgtgttaa gtagttacgt
     6301 acgtttaaca tcaatgaacc tgaacccatc gttggatcaa aaacactaaa gaatcgtcgt
     6361 tccttttgat cgagcgttac gatttgcgcc atcatatcag aaaccatatg aggtgtgtaa
     6421 aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ctcatacgca
     6481 tccccgatca cgtccccatc gtgttctaaa acatctacat cattcaattt cttgatgact
     6541 tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc
     6601 acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg
     6661 tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga
     6721 tactctggtg caattgcata tcccaaaata tcaacgattg tggcaatcag atcttctttt
     6781 gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac tgtgtcatat
     6841 tcctctaagg attcatctgc taaaagaacc acttg
//
LOCUS       DACCIQ010000113         6640 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCIQ010000113 DACCIQ010000000
VERSION     DACCIQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.116"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            242..2008
                     /locus_tag="IVM62_002497"
     CDS             242..2008
                     /locus_tag="IVM62_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP6846413.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2110..3369
                     /locus_tag="IVM62_002498"
     CDS             2110..3369
                     /locus_tag="IVM62_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6846414.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3516..4817
                     /locus_tag="IVM62_002499"
     CDS             3516..4817
                     /locus_tag="IVM62_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6846415.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4820..5671
                     /locus_tag="IVM62_002500"
     CDS             4820..5671
                     /locus_tag="IVM62_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6846416.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5684..6499
                     /locus_tag="IVM62_002501"
     CDS             5684..6499
                     /locus_tag="IVM62_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP6846417.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         2126 a         1147 c         1341 g         2026 t
ORIGIN      
        1 ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca
       61 ctaaatagta caggaagtag actaaaactt ttaaaagttt taagtctatc tatttggcaa
      121 gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt
      181 gtcttttgct tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc
      241 aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa
      301 tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct
      361 atgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa
      421 aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag
      481 tcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga
      541 tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat
      601 accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg
      661 gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac
      721 tttaccttgg ggaagtaaag atccagaccg agaagacttt ttcgggggag atttgcaagg
      781 tgtgatggat catttggatt acttgacgga ccttggcatc aatggcatct atttttgtcc
      841 catatttaaa gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc
      901 cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat
      961 acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga
     1021 cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc
     1081 agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac
     1141 ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata
     1201 tttgctggat atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga
     1261 tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat
     1321 caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg
     1381 ggatgaattc catgcagtga tgaattatgc atttacagat tcgattaaag attattttgc
     1441 aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta
     1501 cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat
     1561 cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct
     1621 tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga
     1681 cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg
     1741 ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc
     1801 tactcagtgg ctaatagtaa atgatcgtga agataagctc tatttcacac ggaaactaga
     1861 gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga
     1921 aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg
     1981 acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt
     2041 gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa tacaaaggag
     2101 gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga
     2161 atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat
     2221 gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata
     2281 aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc
     2341 gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg
     2401 gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg
     2461 ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc
     2521 tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat
     2581 acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt
     2641 gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc
     2701 tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca
     2761 gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc
     2821 aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag
     2881 caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac
     2941 aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct
     3001 tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac
     3061 ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa
     3121 atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat
     3181 gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca
     3241 gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca
     3301 aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa
     3361 aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat
     3421 aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca
     3481 gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata
     3541 tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg
     3601 gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc
     3661 ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag
     3721 atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca atcattttct
     3781 tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg
     3841 gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc
     3901 cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc
     3961 tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt
     4021 taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta
     4081 atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc
     4141 aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac
     4201 gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga
     4261 gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca
     4321 taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga
     4381 cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg
     4441 ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag
     4501 caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc
     4561 caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta
     4621 acaatggcgg tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct
     4681 ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt
     4741 tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt
     4801 tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact
     4861 ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca tctatccgct
     4921 tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct
     4981 ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac
     5041 ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt
     5101 aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat
     5161 gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat
     5221 ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg
     5281 tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt
     5341 agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt
     5401 accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg
     5461 ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg
     5521 cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc
     5581 aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac
     5641 cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt
     5701 aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc
     5761 atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac
     5821 tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc
     5881 gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt
     5941 acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc
     6001 tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat
     6061 ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca
     6121 accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc
     6181 cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt
     6241 catgacgagt ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca
     6301 actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat
     6361 aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag
     6421 tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa
     6481 agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat
     6541 gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt
     6601 tcttttttag tattaacgta atagggtagt ttttttaagg
//
LOCUS       DACCIQ010000114         6563 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCIQ010000114 DACCIQ010000000
VERSION     DACCIQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.117"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            67..234
                     /locus_tag="IVM62_002502"
     CDS             67..234
                     /locus_tag="IVM62_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846418.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(277..852)
                     /locus_tag="IVM62_002503"
     CDS             complement(277..852)
                     /locus_tag="IVM62_002503"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6846419.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1007..1533)
                     /locus_tag="IVM62_002504"
                     /pseudo
     CDS             complement(1007..1533)
                     /locus_tag="IVM62_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1990..3501
                     /gene="gadC"
                     /locus_tag="IVM62_002505"
     CDS             1990..3501
                     /gene="gadC"
                     /locus_tag="IVM62_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP6846420.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3514..4914
                     /locus_tag="IVM62_002506"
     CDS             3514..4914
                     /locus_tag="IVM62_002506"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP6846421.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5079..6515)
                     /locus_tag="IVM62_002507"
     CDS             complement(5079..6515)
                     /locus_tag="IVM62_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6846422.1"
                     /translation="MLETYLEKSIFRQVYICEQLHEKGTIQIREIADQLNVCPLTVTN
                     DLECITDILHQQIISVEKSRSSFTVAFDSSFSRLALTQLIYQRSDFFNVLGHYLSGEH
                     NWSNIADTTFISLSKVYNLRNELINFFKQMQYLNADGHFEIPEKDYRSLLLTIIYETN
                     RTELIQLNKSIIQSGQQLIDYVEKHFFSRSYPTVEKQFILLGIAIGLQRSKTNPIYFS
                     QEEKRVARHTPLFQLIQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKDL
                     NVVYQNHVADNPLVIELSQKLILSLNISEENQILFDKALLPFIRSLWADIQIFQPDKT
                     YLLNEEQRALYLEVHEILEQWRKQNNLFLRWNENSIRKLTISLSLLNEHKRKSPIEVF
                     IVAPSDFRYLYYRQQLEDILGEHFSISNIICKQLREIVDDTFFCTQRIILCDSSLYQE
                     GLGSEKTIIYPITFQTIHTVIDQLNKQI"
BASE COUNT         2025 a         1133 c         1185 g         2220 t
ORIGIN      
        1 tgctcttttc tgcggctgta tttcttgatt tgtttattga aagattcgat tgtagaacca
       61 gaaggaatgg agaacacttt tgaactttac aacaaaacta ctgttgataa attaattcca
      121 attttagttc caactgattt aaatggtcat gatgttttac ttcgttttaa cggattttta
      181 ggtgaggatt atagatacct aaaaatttct gatcctcaac ctttaacaga ataataaaaa
      241 acgtgtaatc aattatttga ttacacgttt tttatttcaa tcttcgttgt agtagtggtt
      301 gcgacatacc gaaaggtaat cttcattacc tcctactaca atctgttcac ctgaataaac
      361 tggaatacca tcaacaatac gagcattcat tacagccttt ttattgcaaa aatggcaaat
      421 agttttaatt tcctcaattt tatcagcaaa tgtcaataat gcttcggatc ccttgaagag
      481 attattttga aaatcattct tcaatccata gcaaattact ggtacttcct cattcattac
      541 gataagtgat agatcaataa tattctctct acttaaaaat tgagactcat cgaccaaaat
      601 acaatcaact tttccgtact gatcaataat ctccactaat tctccgtaca catccatttc
      661 agaagtaatt gcaatagctg gtttcgaaac acctatacga ctctcaactt ttccaaatcc
      721 tgatcgagta tctattgcgc ttgtcattat gataactctc ttcccttgtt cttcataatt
      781 atgagccact ttcagtagct caatactctt tccactattc attgttccat atctgtaaaa
      841 taattgtgcc attaaattta ttcccttctt ttacaattca attattcctt aattccatct
      901 gcttttatgc agctgctcgt ttattccttg atatgtctat aacatctact atccctaaaa
      961 ccaatttata agccattaga gatggtattc ctgaacatct ctcatattgt tttttgagtg
     1021 gtatatcatc tgaaaaactg aaggaatcat gaaaatagcg acatacttat catttagagt
     1081 tctaacttta aatgcattta cttcttcagt cagcacttta gtcatgttgg acatggtttg
     1141 gggcgtgtag tgatgcccat acattttctc aatcaagtct gcgatttccg acatggtaat
     1201 accttttcgg aagaggtgaa tgacggtctc ctctaatgtg tcattcgtcc gtctataagc
     1261 aggaacagtc tgttgcttga actcgccatt gcggtcgcgc ggaatctgga gatgaagttc
     1321 cccatactcg gtcttgaccg tacggtcata ggagccgtta cgagaattac ccgtgttaaa
     1381 accaatgcga tcgtactttt cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt
     1441 attgacagct ttttccaaat ggacacggaa taatccattc aaatcgcctt tgttcgctag
     1501 agtctttagg atttctgtag taaaatcgtt catatggaag tcctcttttc tgtgaatgtg
     1561 ttgtggtaac tctattctac agaagggact tccttttttc tatttacaca aaatatttta
     1621 cactctcaac aaaagtatct tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa
     1681 gtaggtaccg aggtactttc tttttcgcag ctttttttgg acttatgaga agtacattcg
     1741 ctggaagaat acgatataaa acgagctttt tcactaaaga tattaataga gtgaaagaaa
     1801 caagcttttg ttgtcagttt tttcactttt ctaaaaatga aaataaaaaa ttctggaagt
     1861 ggagaagaat tttgcttgct atactttgtc ttgtagaaaa gaggtagaca aaataatgca
     1921 agctcaaata agcgattttc cataaccttg gtaggaatga caaattaaat aggaaaggga
     1981 agtaataata tgaataaaaa aaagttatct ctattcggtt tttttgccct gacagcttcc
     2041 atggttttga ctgtttatga gtatccgacc tttgcaacat cacaattaca tttggttttc
     2101 tttcttcttt tgggaggtat tttatggttt ctaccagttg ctttatgtgc tgcagaaatg
     2161 gcaacagttg aaggatggaa agatggtggg attttcagtt gggttagtca aactttagga
     2221 gaacgttttg gttttgcagc tatttttttc caatggtttc aaattacagt aggttttatt
     2281 acaatgatct acttcatact aggagcgctg tcgtatgtaa ttaattttcc agagcttaat
     2341 acagatccat ttatcaagtt tattggtgta ttagtcattt tctgggtact gactttttca
     2401 cagttgggcg ggaccaatcg taccgctaaa attgctaaag caggatttgt cattggaatt
     2461 gttattcctt cgatcattct ctttggttta gcagcagcct actttatcgg cggtaatccg
     2521 attcaaatgg ctattaatgc gaaagctttt attcctgact tttctcagct ttcaacttta
     2581 gtagtatttg tatcttttat attggcttat atgggcgtag aagcttcagc atcacatatt
     2641 aatgagctgg aaaatcccca aagaaactac ccattggcaa tgtttttact agtgattttg
     2701 gccatcgcct tagatgcaat cggcggtttc tcagtagcag cggttattcc acaacatgac
     2761 ttgtctctca gtgctggtgt aatccaaaca tttgaaacgc ttattttaca ttttaacagt
     2821 catttacatt ggttggtaaa agtgattgct ctcatgattg cctgtggcgt tatgggtgaa
     2881 gttagttctt gggtagttgg tccatcacga gggatgtttg cagctgctca gcgaggtctg
     2941 ttaccagttt tcttccggaa aactaataaa caagacgttc cagttccgtt gattatgata
     3001 caaggaatta tcgttactat ttgggcagca gtcttaactt ttggtggagg aggcaacaat
     3061 ttatcattct tagtagcaat ctccttaact gttgtcattt atctcgtagg atatttacta
     3121 tttttcctag gttactttgt attagttttt aagaaaaaag acttaaaacg aacttacaat
     3181 gtaccaggaa aaactgttgg caaaacgatt atcggtggaa ttggttttat tttatcagtg
     3241 ttcgcattgc ttatctcttt tgtaccacca gcttcaattg cagctagtga gcaccgaaca
     3301 taccaaatca tactacttat tagctttgta gtgactgttc ttttaccttt tatcatttat
     3361 gaagtacatg ataaaagtgc tcacacaact attgaggaac ctactcacgt gaaagctgaa
     3421 gacgttaatc ccgctgttta tccagctgct agaggagagc accacgttac aaaaaatgat
     3481 gaacatattt taaaacatta ggaggaattc attatgttat acggaaaaga taatcaagaa
     3541 gaaaaaaact atttggaacc aatttttggc tctgcaagtg aggatgttga cttgcctaaa
     3601 tataagttaa acaaagaatc cattgaacca cgaattgctt atcaattagt acaagacgag
     3661 atgttggatg aaggaaatgc gcgattaaac ttagctactt tttgtcaaac gtatatggaa
     3721 cctgaagcag tgaaattgat gacccaaacg ttagaaaaaa atgcaattga taaatcagaa
     3781 tacccacgaa caacggaaat tgaaaaccgc tgtgtaaata tgattgctga tttatggcat
     3841 gctccaaata atgaaaaatt catgggaact tcaacgatcg gctcttcaga agcctgcatg
     3901 ctgggtggta tggccatgaa atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat
     3961 attcaagcaa aaaaacctaa cctggtgatc tcttctggtt accaagtttg ttgggaaaaa
     4021 ttctgtgtat attgggatgt ggaactgaga gaagtcccaa tggatgaaaa acatatgtca
     4081 attaatctag atactgtcat ggattatgtg gatgagtaca caattggtat tgtaggtatt
     4141 atgggtatta cttacactgg tcgttatgat gatatcaagg gtctgaatga tttagttgaa
     4201 gctcacaata aacaaactga ctataaagta tacattcatg ttgacgctgc atcgggtggc
     4261 ttttatgcac catttactga acctgatcta gtttgggatt ttcaattgaa aaatgttatc
     4321 tcaattaatt cttcaggtca caaatatggt ttggtatatc caggtgtggg ttgggtctta
     4381 tggcgtgacc aacaatactt accagaagaa ttagtattta aagttagtta cttaggtgga
     4441 gaaatgccaa ctatggctat caacttctct catagtgcag cacaactaat tgggcaatac
     4501 tacaactttg tacgctatgg ctttgatggt tatcgtgata ttcaccaaag aactcatgat
     4561 gttgctgttt atttagccaa agagatcgaa aaaactggta tttttgaaat cattaatgat
     4621 ggatcagaat tgccagttgt gtgctataag ctgaaagaag atcccaatcg cgaatggaca
     4681 ctatatgatt tatctgatcg tctgttaatg aagggatggc aagtcccagc ctacccatta
     4741 cctaaagact tggatcaatt aattattcaa cgcttagttg ttcgagcaga ctttggaatg
     4801 aacatggctg gtgattatgt acaagatatg aaccaagcaa ttgaagagtt gaataaagct
     4861 catattgttt atcataaaaa acaggatgtg aaaacgtacg gctttactca ctaaaattct
     4921 tcagagaaat ataaaagaag ttaattaact aataaaagga gttgtgatta ccacacagct
     4981 ccttaaatct atttgaataa atatatgata ttcatttgtt acactctcaa tcttagctgt
     5041 tgttctattc atcattactc ttattgtaag tagttcagtc aaatttgctt gttcaattga
     5101 tcgataacag tatgaattgt ttgaaaagta atcggatata taattgtttt ctcactacct
     5161 aagccttctt ggtataaaga actatcgcaa agtataatcc gctgagtaca aaagaaagtg
     5221 tcgtctacta tttctctaag ttgtttacaa atgatattac taatagaaaa gtgttctccc
     5281 aaaatgtctt ctaactgttg tcgatagtat aaatacctaa agtctgacgg agctactatg
     5341 aacacttcaa ttggagattt ccgtttatgc tcatttaaaa gacttaaact aatggttagt
     5401 ttacgaatag aattctcatt ccaacgcaga aataaattgt tttgttttcg ccattgttca
     5461 agaatttcat gaacttctaa gtacaaagcg cgttgctctt catttaacaa gtaagtctta
     5521 tcaggttgaa atatctgaat atcagcccaa agtgagcgga taaacgggag aagtgcttta
     5581 tcaaaaagta tctgattttc ttcagatata ttcagagata agataagttt ttgactgagc
     5641 tcaatcacca aagggttatc tgctacatgg ttttgataaa caacgttcaa gtctttttgt
     5701 aacaattcaa agttatcgca tagataattt ctagagttga atagaaagta gatataaagg
     5761 cattcatctt cgtcatgaca taaagagaaa gaaagttgct ctaatccttt ctgtattagc
     5821 tgaaacaagg gagtatgtcg ggcaactctt ttttcttcct gactaaaata tattgggtta
     5881 gtcttagatc tctgcaaacc gatagcgata cctaataaaa taaattgttt ttcaactgta
     5941 gggtaacttc ttgaaaaaaa atgtttttct acataatcaa tcaattgttg accgctttga
     6001 ataattgatt tatttagctg gatcaactct gtccgatttg tctcatagat tatcgttagt
     6061 aacaaacttc gataatcctt ttcaggaatt tcaaaatggc catcagcatt taagtactgc
     6121 atttgtttaa aaaaattaat caactcattt cgtaagttgt aaactttgct taacgaaatg
     6181 aaggtagtat cagctatatt actccaattg tgttcccctg ataaataatg tcctagtaca
     6241 ttgaaaaagt cagagcgctg atagatgagt tgagtgagtg ccaacctcga aaacgaagag
     6301 tcgaatgcga ctgtaaagct acttctagat ttctccactg aaataatttg ctgatggagt
     6361 atgtcggtaa tgcattccaa gtcgttagtg acagtgagtg ggcaaacgtt caattgatct
     6421 gctatttcgc gaatctgaat ggtccctttt tcgtgtaatt gctcacaaat gtaaacttga
     6481 cgaaaaatac ttttctccag ataggtttcc aacatagtac tcctcctagt cattttatac
     6541 ataactatta taactctacc aat
//
LOCUS       DACCIQ010000115         6396 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCIQ010000115 DACCIQ010000000
VERSION     DACCIQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.118"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(52..279)
                     /locus_tag="IVM62_002508"
     CDS             complement(52..279)
                     /locus_tag="IVM62_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846423.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(446..2416)
                     /locus_tag="IVM62_002509"
     CDS             complement(446..2416)
                     /locus_tag="IVM62_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846424.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2431..3297)
                     /locus_tag="IVM62_002510"
     CDS             complement(2431..3297)
                     /locus_tag="IVM62_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846425.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3287..3520)
                     /locus_tag="IVM62_002511"
     CDS             complement(3287..3520)
                     /locus_tag="IVM62_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6846426.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3532..5688)
                     /locus_tag="IVM62_002512"
     CDS             complement(3532..5688)
                     /locus_tag="IVM62_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846427.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5708..5929)
                     /locus_tag="IVM62_002513"
     CDS             complement(5708..5929)
                     /locus_tag="IVM62_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846428.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6039..6377)
                     /locus_tag="IVM62_002514"
     CDS             complement(6039..6377)
                     /locus_tag="IVM62_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846429.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
BASE COUNT         1802 a         1262 c          876 g         2456 t
ORIGIN      
        1 gactgagatc acctttcaat ctaaatacac ggttcttttc tggtaactta ttcatgcttc
       61 aaccacctca tactgttctc tcataccttt gacttcatta aaaaaaactc tcgctttttc
      121 ttcatcagta aagtttttat acaactgatt acaataatga ggtttttgaa gtttctgaat
      181 acctatacta acttcaaatc cttcttcatg ttgaattagg attacttctg tgtctctacc
      241 tagaaatatt cgatctaaaa tttttatgtt tggtttcatt atttaacctc cactataatt
      301 atttcactca ataaggtttc actttatgaa atttctatag ttctcttttc acaaaacgta
      361 ccatgttctc taagactgtt tttagtctca aatatacatg ttttttctta tcctttcctt
      421 atacactcaa aatcaaagat aaaacttaaa gaataaactc tccaataaca aaattattag
      481 tcagatccac tggtctcctg atctgaaatt tattacttct tcctgttgtc aaatactgtc
      541 tcaaatcaac agaaagtttt tgatcatctt ctccttttct agcaggagca actttttttt
      601 catacttcat taactctcca ctggtcatct ttccaatgat agtgtaatta aaatcacttt
      661 caaaactttg gtaaattttt ccatctgtat tgaatatatc agctgtttca atatcttctt
      721 tgtattcatt gtcttctcct gaaccgattg tatcaaatag aaaggcaaga cgaattccat
      781 cttcttcaag cattgttaat ctttttttaa ttagcccaac ggtttcaata gataacttat
      841 cgaagccgtg aatcataaca atatcatctt tttttgcttg tgatgtgatg aaatcaaagg
      901 tatttaaaaa ttgagcttcc ataacattgg tttctgctct tagatcacct aagttatagt
      961 aaaactgtaa tttgtcactt ttaccgattt ctttcagtgt cgttctttga ttaaacatcc
     1021 tagagaagct gtctaatgct gttctcaatg tgtcttttaa tacaataata cgatcgactt
     1081 ctttttcagt accagatttt ttggcttttg tttcagcatt agtcaaatag ctaataaaat
     1141 ccccaagaac tggcattgta tcactactgt tcaccaaaag ttttgaacgt tcttcataat
     1201 taatatccca aagtttctga cttatataaa agttacctaa ttcttttttc aataaaactc
     1261 tagttgcaga atctaatgtt ctgcctgcta acaaataaaa aatctgagtt agtttatcga
     1321 gattccgtct atataaatca ccaatgtcct tttgattacc aaaaagctcc aaagagttaa
     1381 gaccttcttc agccaaatta accttttcta ttacactgtt taacgaaggt ttggcaacaa
     1441 atgttctacc agttggatca ccaaagtaat caaatttatt taagacaata tgaaaagttc
     1501 gtttatgatt tgccattgca ttattagcaa tcaattgccc ccaaagtgat gatgctgaga
     1561 ttttttcatc atagccttta attgtagcgt ttttgtgtga tgccactaaa actttacttg
     1621 taaaagaatc atccagtgcc ataaaagcta caccacttgc gtagctagta gacatagtgc
     1681 cgatagccca tccattcgca tcatctaaac ctttgcgaat tgcatgatca tttcctgcaa
     1741 aatccgaact catagacgta tactcataga cattcccgat tggttcactt aaaagtctat
     1801 caaacagatc acgttgatct cctccatatg acataagctt taatccatac attttttcat
     1861 catacaaccg ttggagttta attagctgtt cgctgatttt attaggagaa tcggatacta
     1921 ataggatttc tagcttccaa tcaataattt tatttgccat gctgtctagt aaagatgctg
     1981 tattcaaatc cataatccgc ataacagttt tgctaatttc agcatctgaa ttttttcctt
     2041 ctaaattatt ttcataacta ttcgttgatc ccccaacatt ctcgtgcatt atttcttgtt
     2101 gatctttttg tgacactggc ttatctgctt caattagata ggcttttacc ccttctactc
     2161 tcacttcagg aatagcttta accatccaac caaatccata atttctattc attccataag
     2221 tattcactaa tttcacaatt gttccccacc gcttacctgt cctaatgtaa gatggtgtaa
     2281 attgataacc aactaaagat gttaaatcac taacaaaggt attattcact acctttgaat
     2341 ttctagtttt ttgttctttt ttagtttttc tctttttttt gaaatttaat agttgtgatc
     2401 tacccataat ttccatttaa tctctcacct ctatttcagt gcataaatat cagataatat
     2461 ttcttttgtt tgtttagctg acaactgagt gacactataa taaagtcctc tactcgtata
     2521 gttttctaaa cgttccacac cttcatccaa taccatctta gattgagatt tcaagataag
     2581 atattggatt actgttgttt tcacaccatc aatatattct gatacaaatc gttcttgctg
     2641 atctattatg tcaaagactg ctttgttact ccttgcattt cgtttttgta ttttcatatt
     2701 tcgagtttgt ctttccgtat tctgaggttg agatgaagtg attttgattt cagctaagga
     2761 acgatctctg gtattttgat attgtattga tttcgattcc tgagccataa cttcttctgg
     2821 aaaagcggtt agtgatgtag ccccatctag attgaacatt aatgctacac tcccatctcg
     2881 atatacgatt gttccatctt gtttcacttt taaaatctct gtgcctcgaa atggtttctt
     2941 tttccccata gatactcttg ctgaattaac tactgatttg aacagtctaa tagaaccaga
     3001 aatcaatgaa ccactagatg gatcaataaa catatactgg ctaattagaa taagaaatcc
     3061 aattatcatt aataacgttg gaacaaaaat aaatttcagt ttaatcgtat cagaaactaa
     3121 gtaaacaatt gatccaagaa caacgactgc aacaactata gctgatacaa ttaatctttt
     3181 tgctgtaaca gaataggtgc tattgacatg cttttcaagc cattcaactc tgacaattgt
     3241 ttcagaaggt ttttgatgta agttatgttt aaatccatag tttgagttat ctgccatcag
     3301 aaatacttcc tttcacgttc aacttacggt aaacacctac agttatcact attgcaacaa
     3361 taagcaagcc aattaaagat attgacttgc tcattgtctc gttcgtttgt ggtagttcat
     3421 ttacctgata cacgagctgt gcttcctgta agtgctgaga agaggtcatg ttttcttgat
     3481 tgttttgaat tactggataa actatttttt cttctcgcat aatagccctc cttagttatt
     3541 atcagataag tgagcttgaa ttttgtactg atttccgtct ggagttttca caactgcttt
     3601 ttctccatca atagaaatca cttcagcctt agttgttttc ccatcaatac tgatgacaac
     3661 atctttccca actttgaact ctgtagttgg tgttgcttca tagctactat ttgttgaagc
     3721 agagttcgta tttgtcccaa tcgaattttc cactctcgaa atagatgttt gagtttgagc
     3781 ctgtgcttgt tgattattat gactaataaa gtaggacgat cccaagatcg ttccgcaagc
     3841 cattgcagcg acagaaatga tagtagccat ctttttactt gtaaaatcac gttttgaact
     3901 tgattgattt tctgtagcaa ctgctaatga tttcgttttt tctgccattt gagctagatc
     3961 acttcgggaa cgagataagt cttctcttaa agcttcactt tgttctgcca acttttcgct
     4021 atttaattta taaccctctg ctaaatcttt gacctctttc attcgatctt tatattcttc
     4081 gatagttttt tgttgctcta caagttttgt ttctaattct ttcgtgttgt tagctacctg
     4141 cttttcaact tcttctttgt tcgcaaaata tttgacaacc acatctgttg ttttctcttt
     4201 tgtatcatgg aactcagctt cattttctga ctttgttttt tcgacaatct ctaaagtttt
     4261 ttcttgcacg tctgttaatg atttttcaat ttctggtttc aatgaattga taattcgttc
     4321 caccacaact tcttttgctt cttggctttg tttcaatgca tcgtttttaa tagattcaat
     4381 tgcttcttgg tactgttgat caagactttc ttcagttgct tttctatatt catcttcttg
     4441 ttgtttaact tctggtaaat gctcagcatc ataccgttgc tctagtttct ttagttcagc
     4501 atccaaataa gcctgtttcg ccatatcata atctttacga atgatattgg atttttccat
     4561 cactaaatca tcaattttag atgcttctgc ttcgtagctc tctttagcaa ccgatagtgt
     4621 atttgtaaac tgattttgac catttaatga ggttgcttcc tctatccgca aaagctcatc
     4681 agctaacatt tgctttcctt tgaggatcaa ttgctccttc gtcttattta attcactgtt
     4741 gattttatta ttagcaatag agatgcgagc gttcatactc tctttagcta aaaacagcaa
     4801 tggtttcaac tcaggtatgg atagcatctc ctcgtttaaa tcgacagggt cattaatctc
     4861 atctaaaact acaggttgaa atttgtcagc aatgatctgt tcaaaatttt tttctaaaaa
     4921 atcagcatct aagtttttcg gtacatcact ttcagatgtg attttttcag cttcataaac
     4981 ataattttct ttgtcacttt gggattcggg tttttcatca atttttggtt gttggtaggt
     5041 ttctgtttgt atttgtgttt cttctatgct tacttcttgg ttttctgctt cggttggttt
     5101 gttatcatcc acaaaaagag cttctttttc atctggtaca ttttctgcta cggcttcaaa
     5161 ttcaacttct tcagaagagt tgaaggtctc agcaattcgt tctaatccat tagtagctac
     5221 ttctaattgc tctactgcgc tatagaagtc tgtagcttct tcaacttctg attcaaacac
     5281 attaggatca tccggaataa ctaataattg accactacta tccataaaac ggacacctgc
     5341 taatagcttc tctttaaatt tttctggtag atcagctaca taattcaaac ttgacttcaa
     5401 tagaacgaca tacttctcag aatcgagaat agcatcatcc tcatctataa ctccctcact
     5461 gttgagcttt tgtacatctt caattaccat ataatttttc tttggtaaat ctttggtcag
     5521 caacgcatcg aattctttcc atacctttgc tttagctttt tttgccttcg cttctttttc
     5581 ttcttttttg tacacttcga gtaattgtgg cttcaaagag agtgcaacta caggaactgt
     5641 ttctgccaca taattaatag attcggtaat atctttgaat tttgccatta ttgtaaaact
     5701 ccttttttca gtcaatagtt cttttctgtt tatctagcat atatacatat gctttttcga
     5761 atttaataag cgtttcaagt ctggcttttt caatcttttt actcccattt cgataatcat
     5821 agaactggtt aacattcata ttcattatct ttgccagttc ggtacctttc agactgctca
     5881 ttaatacttc gttggcgtga gctaacattt tttctctatc aaaaaccatc ttacatttac
     5941 tttcttccct cacttctaaa ctacgcttgg agcgtagttt tttggtaaaa agtaaattga
     6001 acaaatcggc atattatcca aatttgttca attcactttt aacctaacag ttcatttttt
     6061 ggaaattttg aaactagata gataccaaca actggaataa gtgctgatat gatattagct
     6121 ttttggttca aatcattacc tgttttagta cccattgtgt aaagcataac ggttgtaatt
     6181 acaggaataa taaagattgc aataatcgcc ccaacatatt ttaatccatc tccccatttt
     6241 gcatcaccaa aaaatttcat tgcagtgaat cctggtttca acgaaaaaac gaatgccaag
     6301 aaatacaaaa caacttgtac taaagttttc atatcaggcg tcattccgct aaccaaattt
     6361 ttaatagaat cgaacatata aaaacctcct ttttaa
//
LOCUS       DACCIQ010000116         6258 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCIQ010000116 DACCIQ010000000
VERSION     DACCIQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.119"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(112..789)
                     /gene="nth"
                     /locus_tag="IVM62_002515"
     CDS             complement(112..789)
                     /gene="nth"
                     /locus_tag="IVM62_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP6846430.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(796..1494)
                     /locus_tag="IVM62_002516"
     CDS             complement(796..1494)
                     /locus_tag="IVM62_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP6846431.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1762..2946
                     /locus_tag="IVM62_002517"
     CDS             1762..2946
                     /locus_tag="IVM62_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP6846432.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2952..3395)
                     /locus_tag="IVM62_002518"
     CDS             complement(2952..3395)
                     /locus_tag="IVM62_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6846433.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3621..3908)
                     /locus_tag="IVM62_002519"
     CDS             complement(3621..3908)
                     /locus_tag="IVM62_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846434.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4079..5431)
                     /locus_tag="IVM62_002520"
     CDS             complement(4079..5431)
                     /locus_tag="IVM62_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP6846435.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5704..6150)
                     /locus_tag="IVM62_002521"
     CDS             complement(5704..6150)
                     /locus_tag="IVM62_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6846436.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1687 a         1250 c         1044 g         2277 t
ORIGIN      
        1 agctactgag tatgtacatt tactttttca tgcaaaaaag ataaacgaaa cagcttttct
       61 ccataataac aagataattc atacatttat ttgcaattaa agaaaccacg tctacaggcg
      121 tggtttcttt tttagcgcct tttcttttag acgcatccga tttttcccat cttgacatat
      181 tgatagcaaa ggacatacct cgcacttggg atttcttgcc gtgcaatgat atctaccaaa
      241 aaaaatcagt gtatgatgtg tctttaccca taattcttgt ggtactttcc gcatcagtgt
      301 ttcttctact tccatgaccg tagcatctag tttgcagatc ctcaggcgtt ttgagacacg
      361 ttccacgtgg gtatctactg cgattgctgg tatcccgaat gcatccccca atacgacatt
      421 tgctgtcttt ctgcctacac caggaagaga catcaattct tctcttgacg ttggcacttg
      481 cccatcgaaa cgttcaatca gctgctgtgc acacgctttg atgttttttg ctttatttcg
      541 atacaaacca atcgttttta ttttaagaat gatttcatcg attgaagctt ctgctaacgc
      601 atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct ttattcactg atacatctgt
      661 cgcctgggca cttaagatca ctgcaatcaa taactcaaat ggattattgt gcttgagttc
      721 tccatgtgct tcgggaaaca ttccatacat cgtttctaga gcttccattg ttttttgttt
      781 gcttaacata ttccgctact cctccaacca atttttcagt gagactttcg gcaattcttc
      841 ttgttgagct gactgttcta cttctctttg aagaagctgc tgttttcttc gtttttggtc
      901 ttcttccact tgttgttttg tacgtagatt ttttctttcc cacgatagca aaatgcggtc
      961 aacgtaatta aaactataag cctgatttaa tacggcttcc cgcagtgcta gcttcaaaat
     1021 ttccggctga taatggtctt cttctagcca ttgtccgatc cgctggaact cgatcgaaga
     1081 aagcgggcgt ccaaattctt gttcaaataa ttgatacact gactggattt ctttttcttg
     1141 tgttttttct tcgtattttt tttcttgtgt ttttaaatac tcacctaata gatcatagat
     1201 ggggtaaaga ttatatcgat ctgctttttt accgttgaca atcgtcgttt ctatcttgat
     1261 aaatccattt gttacgagac gatccagtat ttgaaaaatc tgatcttgtt tgattcccat
     1321 atccatagca atcatctgca aatcagggaa agtatctcct tcaagctgtg ccatatgcaa
     1381 ctgtagaaca aataaaaatt cttcagattg tagaccgatc ctatgataat ttttaagtaa
     1441 aagattagat atgactgttt gtccagcgct taagtaattt tctaaattca acatgatttc
     1501 tcctccgctc aatcacagta tacgtaaaaa aaacatggga agcaagaaca ggtttctttt
     1561 ctatttactt cactgatcgg tcagatttac aatgtcgtct acttgatttt tgtttgttca
     1621 tgtttttgat gcgtatcttc acttttcgga ataaataaat taagtagatt tcgctgttcc
     1681 ttagaatgtt gacaaggaaa caaaattact atacactatt tttgtttcca agttaacatt
     1741 attgcataaa ggagttttta catgtacagt ttcacaaata aacattctaa agtttttgga
     1801 tacgtctctg tcttattcac tggattagga tttactatcg tcagtcctgt acttccgttt
     1861 ttaacgctgc cttattctca ctccatacat cagcaagctt tctatatcac tttgctcatg
     1921 tctgtttatg ctctagctgc cttcttatct gcccccattc tcggtagctt aagtgatcat
     1981 tttgggcgtc atccgatcct tattatcagt ttattaggat caagtatcgg atatttgctt
     2041 tttggtttag gaaattcgat atggatgttg ttccttggtc gtatcattga aggattgacg
     2101 gcaggagaaa tctccacctt atatgcttac tttgccgaca tcacggaacc taatgaaaga
     2161 accaaagttt ttggatggat gggtgcttta gtcggtatag gaacaacgct gggtcctatt
     2221 atagggggtc tattagctga gttggggaat tctgttccta tatttattgg agcactcttt
     2281 actttcttaa atgccgttta tggctacaca tttatgctag aaagccttcc aataaaaaaa
     2341 cgttcagtag atttaagttt ctcccatgta agaccttttc accaattaca acaattattc
     2401 aagatttcat ccgttattcc tttattgaca gcaggttttg ctgtttggtt ggcagctggc
     2461 tctcttcaat ctatcttttc tcaattttcg attgacgcct ttcagtggaa ggctgggttg
     2521 gttggtttaa gcttctcttt gattggaatc ttggatactg tctcgcaatt gttcattatg
     2581 cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa gaatagggat gttcagtgaa
     2641 atcctcgcct atctattcat cacattatct ggaattcttc tactgccgat tttgttttta
     2701 tttgggatta tctgttatgg tttcggtgat tctattttta ctcctgtatt caatggccaa
     2761 ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa tgggcggtac ccaaagtata
     2821 caatctctat cgcgcgtcat cggcccgcta atcgcaggac agctttatgc tgcagcacca
     2881 tttcttccga cggtattcgg atttttgctt atgatttttg cctattatca ctacaataaa
     2941 aaatagtagc ttcatttgct acttcgtata aattctctgt atcgaccgat aaaacgaaat
     3001 actgtgtcaa attcttcttt tgacatattt tcaaatacag tagcatctcg ctgtacccat
     3061 ttttgatgga gttgctcgtg tctattcctt attttttgtc cgattggcgt caattgaaaa
     3121 tagatttctt ttttattctc ttcgctttga taagtgttta tgatccctct agctatcagt
     3181 ttttttgtta gcttactgat ggctcctcta gtcatgtaac tagcattcgc taattttgtc
     3241 acgttaggat cagggatttt cccgatatgt tcaatcactt ctagttcatt tagagtaaat
     3301 cctgcaaatt ctttttccat ttctggttta ttacgatgaa taatctcatt agctaaatcc
     3361 agatattctt ctattagcag ttgtctttta tccatttcta caccttcttt ttctttcatt
     3421 atattgtttt tttgtttcct attcaacaaa gataaaaata ttttgtagga aaacccaatc
     3481 tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag
     3541 atagtaaaga gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc
     3601 ggccttttca aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct
     3661 tcatatgtgt actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg
     3721 aaaggaaaac gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc
     3781 tcttgcgttt cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt
     3841 aatttcttcg aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct
     3901 ttattcatct tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat
     3961 gtaacagctt tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg
     4021 tacttttcac aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt
     4081 attgtgcagt ttcgatttcg cctagcgctg cttgtgcaag taggttcaag taattccatg
     4141 ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt
     4201 tattttgaac agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga
     4261 actgagcacc tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt
     4321 cagtagttgg catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta
     4381 gatcgttcaa agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata
     4441 ggtaaagtcc tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat
     4501 ttcccacaag caaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc
     4561 cacttggatt gtagttgact actgcagagt ctccagctgc aaaaatgtct ggattacttg
     4621 tctgcatgta ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga
     4681 tcagttttgt atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat
     4741 ctgttggtgt agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat
     4801 tttcgcctag cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga
     4861 atggtttatc tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt
     4921 ttccagattc gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta
     4981 ctacacgttt tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc
     5041 ataggagtac atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag
     5101 ttgtcatcac taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg
     5161 ccgttactgt tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg
     5221 ctcctaatga agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta
     5281 ctccgccgac ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat
     5341 atactgtcac ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg
     5401 catgagtaca gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc
     5461 ttcgacaaaa ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc
     5521 acaaaggttt gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaagat
     5581 tcttatttta ataatattca cttttatatc taagattgtg aataaaagac aaaaaaataa
     5641 aaaagctagc gtttttttta cacagctagc tttttttagg tataacactt gtagaaactg
     5701 tttttacatt tgcgcctttt ttgctgactc tttacaatcc ggacagagtc catacaattc
     5761 caaccgatgt tcattaatct caaaatcagt cagtctgctt gcagccattt ctatatcttc
     5821 aagccctggg taatgaaaat caacaatctt accgcagttt tcacaaatta catgataatg
     5881 acgctttgaa ctgaaatcaa atcggctaga agcatcgccg tacttcattt cttgaacaaa
     5941 tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc gcaacactca tatttggaaa
     6001 ccgatgttca agtgctttat aaatctcatc agccgttgga tgggaatgat gttcaatcaa
     6061 atattctaaa accgcgtacc gttgaggtgt gattcgaatg ttctcttttt ttaacttttc
     6121 gataacctct tcgactaact tattttccat gtaaacaccc cacgttgtta ttctcgtctt
     6181 atcatactat ttttacattt taaaatccac ttaacatgaa aagaaaattc aattttattg
     6241 tattttaatg aaaaaaga
//
LOCUS       DACCIQ010000117         6062 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCIQ010000117 DACCIQ010000000
VERSION     DACCIQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.120"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            642..1376
                     /locus_tag="IVM62_002522"
     CDS             642..1376
                     /locus_tag="IVM62_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6846437.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1392..2561
                     /locus_tag="IVM62_002523"
     CDS             1392..2561
                     /locus_tag="IVM62_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846438.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2577..3572
                     /gene="lacD"
                     /locus_tag="IVM62_002524"
     CDS             2577..3572
                     /gene="lacD"
                     /locus_tag="IVM62_002524"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6846439.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3651..4577
                     /locus_tag="IVM62_002525"
     CDS             3651..4577
                     /locus_tag="IVM62_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6846440.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4596..5846
                     /locus_tag="IVM62_002526"
     CDS             4596..5846
                     /locus_tag="IVM62_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6846441.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGIDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2037 a          992 c         1233 g         1800 t
ORIGIN      
        1 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
       61 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      121 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      181 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      241 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      301 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      361 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      421 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      481 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      541 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      601 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      661 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      721 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      781 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
      841 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
      901 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
      961 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1021 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1081 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1141 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1201 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1261 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1321 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1381 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1441 ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1501 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1561 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1621 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1681 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1741 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1801 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     1861 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     1921 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     1981 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2041 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2101 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2161 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2221 cttttgtcaa tgagcagaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2281 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2341 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2401 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2461 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2521 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2581 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2641 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2701 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2761 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     2821 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     2881 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     2941 atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc aatcggcaaa
     3001 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3061 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3121 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3181 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3241 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3301 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3361 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3421 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3481 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3541 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3601 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3661 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3721 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3781 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     3841 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     3901 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     3961 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4021 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4081 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4141 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4201 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt ttattagttc
     4261 ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc gttgattata
     4321 taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta acggctaatg
     4381 gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta gcatcaaaag
     4441 catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt cttccagaaa
     4501 atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt atgttaggaa
     4561 aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt caaaaaatag
     4621 ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg gctgcagtta
     4681 tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg atagaaggat
     4741 ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca tcagattcaa
     4801 catttggtat tattggatta gctgttacct actctttggg acacaatcta gcaaagactt
     4861 atggtataga ctcattatca gcaggattat tatctttgag tggatatgtt ttacttacac
     4921 ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt acctcgggtc
     4981 tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg tttgtactta
     5041 aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga gctttcgttg
     5101 ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta atttgttata
     5161 aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa ccactatctt
     5221 tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt ttcttttgga
     5281 tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta ttattgcaaa
     5341 actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac attataactt
     5401 atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt gcacttgcca
     5461 ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag ttatcaatcg
     5521 ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact atattaaata
     5581 taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt acttattttt
     5641 caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg acaactccac
     5701 cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg attcaagtaa
     5761 taacaattgc aataaatgtt ttgatatact atccattctt taaattagat gataatgcca
     5821 agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt agttaagtat
     5881 cgccgtttgt ataattaagc gagacaaata aaaaagccat ttatgttaca ctccaattgt
     5941 aatacccgct aaagcataca aggagagtga acataaatga cttataccca tcttacacca
     6001 aacgagcttg taatgataga agcatatttt catcaagaaa ctccggttgc tatcgttgcg
     6061 aa
//
LOCUS       DACCIQ010000118         5804 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCIQ010000118 DACCIQ010000000
VERSION     DACCIQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.122"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(48..350)
                     /locus_tag="IVM62_002527"
     CDS             complement(48..350)
                     /locus_tag="IVM62_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846442.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(570..936)
                     /gene="ssrA"
                     /locus_tag="IVM62_002528"
     tmRNA           complement(570..936)
                     /gene="ssrA"
                     /locus_tag="IVM62_002528"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1107..1880)
                     /locus_tag="IVM62_002529"
     CDS             complement(1107..1880)
                     /locus_tag="IVM62_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6846443.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2024..2374
                     /locus_tag="IVM62_002530"
     CDS             2024..2374
                     /locus_tag="IVM62_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6846444.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2355..3071
                     /locus_tag="IVM62_002531"
     CDS             2355..3071
                     /locus_tag="IVM62_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6846445.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3071..4519
                     /locus_tag="IVM62_002532"
     CDS             3071..4519
                     /locus_tag="IVM62_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6846446.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4589..5098)
                     /locus_tag="IVM62_002533"
     CDS             complement(4589..5098)
                     /locus_tag="IVM62_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846447.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5088..5804)
                     /locus_tag="IVM62_002534"
     CDS             complement(5088..5804)
                     /locus_tag="IVM62_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846448.1"
                     /translation="MRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSALAN
                     QVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQEL
                     INWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTT
                     ELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSL
                     FPFTFFIHHLQSRRPGRNET"
BASE COUNT         1963 a         1019 c         1136 g         1686 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      361 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg
      421 gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat
      481 tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa taaggattta
      541 agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg aacccgcgtc
      601 caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg attcacttta
      661 cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt ttcaacttac
      721 agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc acatgggcga
      781 tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt tgttttcgtt
      841 tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga aacgcaattc
      901 aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg gttacgtgta
      961 tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa agcgaatcat
     1021 atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga ttttcttaag
     1081 aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa tgcgtggaca
     1141 attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa tccgtcatag
     1201 gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc attcggacca
     1261 tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag acgtttgaca
     1321 cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc attccgataa
     1381 aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga taagatcagc
     1441 aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac atctgaatga
     1501 atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat tttctgatcg
     1561 ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt ttgacattct
     1621 tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa agaaccattc
     1681 atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc tgcttctgta
     1741 cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat agcttttgcc
     1801 acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc aaagaaagtg
     1861 attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt ttatcttaac
     1921 aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg gttcagtcaa
     1981 caatttagag tatactataa gaagagaatt taggagggaa agaatgattt ttttcagtat
     2041 tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa aaggaaatct
     2101 gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc
     2161 tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat tgatcggcta
     2221 ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc tgctagtatt
     2281 tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg acctggaaac
     2341 tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt tctgaaatga
     2401 tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat ggaaaagaag
     2461 ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta gacctgaatt
     2521 tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca agtactgtgc
     2581 cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc ttagagatgg
     2641 gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct cgaatgaaag
     2701 ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt tcagatgatc
     2761 attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa gcgtatctgg
     2821 acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt actttagcaa
     2881 aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg gattatcagt
     2941 actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa aaaatcgaaa
     3001 aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc gatgattctg
     3061 gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa tggtcgcgtt
     3121 gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga ttgaagaaaa
     3181 caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc aaacctatgc
     3241 cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga
     3301 gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg aacgtgtcat
     3361 ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat ggaaaaatct
     3421 gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac aagcacaggc
     3481 gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag cacttgtagt
     3541 tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga acctgttcaa
     3601 aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg cagcattcca
     3661 agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg gtaattttga
     3721 tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg atttcaataa
     3781 aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag
     3841 acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa tcaacggact
     3901 tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt
     3961 gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg attatgaaaa
     4021 aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag aaacattgaa
     4081 aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat tgacattgaa
     4141 agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa tcatggtcaa
     4201 tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta ctttagaaaa
     4261 aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt ctgaacaaca
     4321 aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa atacaaaata
     4381 tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa
     4441 attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt
     4501 agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga tcttctatac
     4561 cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga tcaatataag
     4621 gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt tcttcatgag
     4681 ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt tcatcaaatg
     4741 tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta
     4801 ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga gagatatcaa
     4861 gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca tccaacagga
     4921 catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg atcgcaaaat
     4981 tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta tgctgttttt
     5041 cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca ggtttcatta
     5101 cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa tagactaaga
     5161 atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag tatatctgtg
     5221 aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat cggaattgta
     5281 agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt ctttaagaca
     5341 agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac gatccacaat
     5401 aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat aaaaaggaat
     5461 aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt gttttgcctg
     5521 actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa actgaatatg
     5581 ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt agatgcagaa
     5641 gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg caaatggtaa
     5701 agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca atagagtatt
     5761 gtctgctttt tgtaataaat taatcgagca gtagcatagc ggat
//
LOCUS       DACCIQ010000119         5766 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCIQ010000119 DACCIQ010000000
VERSION     DACCIQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.123"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            318..1448
                     /locus_tag="IVM62_002535"
     CDS             318..1448
                     /locus_tag="IVM62_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6846449.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1540..2295
                     /locus_tag="IVM62_002536"
     CDS             1540..2295
                     /locus_tag="IVM62_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6846450.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2353..4392
                     /locus_tag="IVM62_002537"
     CDS             2353..4392
                     /locus_tag="IVM62_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP6846451.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4501..5652)
                     /locus_tag="IVM62_002538"
     CDS             complement(4501..5652)
                     /locus_tag="IVM62_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6846452.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1970 a         1039 c         1155 g         1602 t
ORIGIN      
        1 gtaataacct tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga
       61 aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat
      121 gttttcttta cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt
      181 cttgttcgaa aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag
      241 acaaactcgc tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa
      301 agatcgattg gtggattttg ggtccttatt tgactttgtc catgatcggt cttttagaag
      361 tatacagtgc cagttcttat cgtttattgc aagccgatga aaatacaaaa agtctattat
      421 tgcgtcaact aattttcata tttttgagtt ggagcgtgat cttcttagct cgttcagtca
      481 aactacacta tttacttcac cctaaaatag caggatacgg tttagcctta tcgattttct
      541 ttttagtatt agtaagaata gggatattcg gtgtcactgt caacggcgca caacgttgga
      601 tctctctgtt tggcattcaa ttccagcctt ctgaactggc aaatcttttt ttgatttttt
      661 atttaagctg gttttttcgt gacggaaata gtagcccaaa agatctaaaa aaaccattcc
      721 tgattacagt aggtataact tttctgattt tatttcagcc aaagattgct ggagcattga
      781 tgatcctttc gattgcgtgg gtcatatttt gggcagcggc ggttccattt aaaaaaggga
      841 tctatctaat cgttactttt tctgcattgc tgattggagc agcaggcggg gtattatatt
      901 taggaaataa aggttggctt ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt
      961 taagagattc ttttatcgat agtcatggtg cgggctatca aatgacccat tcgttttatg
     1021 cactatataa tggtggcata tttgggcggg gattaggaaa tagtatcaca aaaaaagggt
     1081 atcttccaga gacagaaaca gattttattt tttcgattat tacagaagag cttgggctaa
     1141 tcggtgcatt gtgtgtgctt ttcttattgt tctcattatg catgagaatc ttttgtttaa
     1201 gcagtcgctg taaaaatcag caagctggat tgtttctttt aggattcgga actttgctat
     1261 tcgttcagac gataatgaac gtgggaagta tcgcggggct gatgccaatg actggtgtgc
     1321 cattgccatt tgttagctac ggaggaacaa gttatctgat cctttcttta ggaatcggaa
     1381 tcacgttgaa tatttcttca aagatacagg ccgaagagtt ggcgctctat cgacctgaaa
     1441 aacaataaag agaaactgag aaactctcag tttgaattat cataaagtac aatcttttat
     1501 ttttcaagcg agaggttctt ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc
     1561 atattatcta ttttgttact gcttacatta gtagttggct atttttcgat tgagtttatc
     1621 catggatttt cgtctgcaaa acagagttca actgtgaaaa aggtagagcc gaaaagtatc
     1681 cctaccacac taaatgtggc tttgattggt tcagatgctc gttcgaaaga cgaaaacggt
     1741 cgctcggatt cacttctggt tgcacaatac aaccagaaaa cacaacaagc aaaactgatc
     1801 tctatcatga gagattcata tgtcgatatt ccaggttacg gaatgaataa gataaatgca
     1861 gcttactctt atgggggagt tgatttatta aatcaaacat taaaggaaaa tttcaaattt
     1921 gaagctccct attatgcaag tatcacattt caagatttta tcgattgcgt taatgaactg
     1981 tttcctggtg gagtcaagat cgatgcagaa aaatctctag atttagatgg cgtatatata
     2041 aacaaaggag agcaagtgat ggatggtaat actttactcc agtatgctcg atttcgggaa
     2101 gacaaagaag gagacttcgg gaggatcaga aggcagcagc aagtgatcaa agctatctct
     2161 cagcagctga aagacgtaac ttcaatattc aagctaccca aagcggttgg gaaattactt
     2221 ggtagtatcc aaacgaacct tccagaaagt gtgcttctgg attgtggtat ggatttcctc
     2281 aaagacatat gttagaataa acaggtataa atagtgaaat aaaggaatga caagcaagag
     2341 aaggaggaaa aaatgaaaag aagtgacaag cacggcaaaa atcgaacagg cgcttatatt
     2401 gccggcgcag tgatcttaat agcagctgcg ggtggcggtt atttttacta ccagcactat
     2461 caagaaaccc aagcagtaga agctggagaa aagacagttg agcaatttgt ccaagctttg
     2521 aacaagggag attataacaa agctgcagaa atgacatcaa aaaaggcggc aaataaaagt
     2581 gcattatctg aaaaagagat cttagataaa taccagaata tatacggtgc tgctgatgtc
     2641 aaaggacttc agatatcgaa tctaaaagta gataaaaaag acgattctac ttacagtttt
     2701 tcatataaag caaagatgaa tacctcatta ggtgaattga aagatctttc ttataaagga
     2761 acattagaca gaaatgatgg tcaaaccacg atcaactggc aacccaactt ggtttttcca
     2821 gaaatggaag gaaatgacaa agtaagcctg accacacaag aagcagcaag agggaatatt
     2881 atagatcgaa atggtgaacc attagcaaca acaggcaaac taaaacaatt aggagtcgtt
     2941 ccaagtaaac ttggagatgg aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc
     3001 ttcgacttaa cagaagatgc tatcaatcag gcgatttcac aaagctgggt acaacccgat
     3061 tactttgtcc cattgaaaat cattgatgga gcaacgccag aacttccagc tggagctacc
     3121 attcaagaag tagacggcag atattatcct ttgggtgaag cagctgctca gctgattggt
     3181 tacgtgggag atatcacagc agaagatatt gataagaatc cagaattaag cagtaatgga
     3241 aaaatcggtc gctcaggttt ggagatggct tttgataagg atcttcgtgg gacaacaggt
     3301 gggaaattaa gcattacaga tgcagacggt gtagagaaaa aagttctgat cgaacatgaa
     3361 gttcaaaatg gcaaagatat caaattgaca atcgatgcga aggcacaaaa aacagctttt
     3421 gacagtctag gaggaaaagc tggatcaact gttgcgacaa cgccaaaaac cggtgatctt
     3481 cttgcgcttg ctagctctcc aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa
     3541 gaagattaca aagcttatga agaaaatcct gaacatccat tcatcagccg atttgcgaca
     3601 ggttatgctc ctggatctac gtttaaaatg attacagcag caatcggtct cgacaacggc
     3661 actatcgatc caaatgaagt gttgacgatc aacgggctta aatggcaaaa agacagttct
     3721 tggggatctt atcaagcaac gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact
     3781 gctttgatct attccgataa tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa
     3841 aaatttcgta caggcttaga taaattcatt tttggtgaag accttgattt gccaattagt
     3901 atgaatccag cacaaatttc taatgaagat agctttaact cagatatctt gctagctgat
     3961 actggatatg gacagggcga acttctaatt actcctatcc agcaagcagc aatgtattct
     4021 gtttttacca acaatggcac acttgtctat cctaaattga ttgcagataa agagacaaaa
     4081 gataagaaga atgtcatcag cgaaacagca gtacaaacga tcgtgccaga tctgagagaa
     4141 gttgtgcaag atgtaaatgg tacagcacat tctctttctg ctttagggat tccgttggca
     4201 gcgaaaactg gtacagcgga aatcaaagaa aaacaggatg taaaggggca agagaacagt
     4261 ttcttgtttg ctttcaatct tgataaccaa ggttatatga tggtcagcat gttggaaaat
     4321 aaagaagatg atgattcagc aaccaaacga gcatcggaac tattgcaata cctcaaccaa
     4381 aattatcaat aaaaaattgc tttttaagta cagtaaaagg actgtggcgt tatcgtcaca
     4441 gtcctttttt caaataaacg gttttcacga tgtctctttc acaacttctt tttgatttac
     4501 tcacacttca taaacttttt ttgtaaaata tttgagaaca agtggagaaa taagtgtact
     4561 taataaaatc acgataacta aaggtgaata gtactgtggt tcgatcaatt gactttgctg
     4621 tccaatttgt aaaatgatca aagccatttc tccacgcgaa atcataccag caccgaccat
     4681 caatgaacta tgttgagaaa aaccggcaat ctgagcacca aaataacctc caagcaattt
     4741 ggtcaaaatc gctacaagcg tcaatataga aataaagagt agttgttcac taaagtcaga
     4801 aaaatcaact tctaatccta cactgacaaa aaatactggt ataaatactg catatcctaa
     4861 agcctctaca ttgtggtata cttcttcttt aacctttgtc tgactgacag ctatcccagc
     4921 aaaaaaagca ccaatgacag agctaagccc aacaagatcc gctacgtaag acattcctaa
     4981 acaaataact agcgatacga tgataataga agaatttgcg tacacttttt ctgcaaatga
     5041 aagtaggaaa ggcgcaatcc atttgactaa taagaaaata aaaacaaaat aaattagctg
     5101 ttcaatcaaa acaagcggta aagaagcatt gctagaagct tcccccgtta gaaaagatag
     5161 gctgaaactt agaactaaaa cgactaaaat gtcatctacc acagaagcac ccaaaattgt
     5221 tgatccttct tttgtattca ctacgtttag ttcttttaat acttctactg aaatactgac
     5281 agaagttgcc gcaagaataa tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc
     5341 tttgcctgtc aaccagccaa aaacaaccgg gaagatgatc ccaagcaaag caacgaacat
     5401 accaggtcgg aaatattttt tcaaaagaga taaatcactt tccagaccag ctaaaaacat
     5461 taataagatc acaccaatct ccgaaaagtc atggactaga atatcaggtt tgacccaatt
     5521 caatccagct tggcctagca aaacaccaac aagtaattgt ccaatcactg ctgggatacc
     5581 taatctccgc gagatatgtg agccaatcgt tgttgaaatc aatatcaggc ataaaatacc
     5641 aataaattcc attctcatca ttcctttgta taaaatatag cccgaactta gcataacata
     5701 cattattgaa aaaataggaa aatatactca caaaaaaacc aacaatccaa aagatttttt
     5761 tggatt
//
LOCUS       DACCIQ010000120         5765 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCIQ010000120 DACCIQ010000000
VERSION     DACCIQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.124"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(228..935)
                     /locus_tag="IVM62_002539"
     CDS             complement(228..935)
                     /locus_tag="IVM62_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846453.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1000..1836)
                     /locus_tag="IVM62_002540"
     CDS             complement(1000..1836)
                     /locus_tag="IVM62_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846454.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1854..3263)
                     /locus_tag="IVM62_002541"
     CDS             complement(1854..3263)
                     /locus_tag="IVM62_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6846455.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3277..5100)
                     /locus_tag="IVM62_002542"
     CDS             complement(3277..5100)
                     /locus_tag="IVM62_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6846456.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5380..5607)
                     /locus_tag="IVM62_002543"
     CDS             complement(5380..5607)
                     /locus_tag="IVM62_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846457.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1764 a         1255 c          943 g         1803 t
ORIGIN      
        1 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
       61 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
      121 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
      181 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
      241 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
      301 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
      361 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
      421 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
      481 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
      541 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
      601 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
      661 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
      721 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
      781 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
      841 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
      901 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
      961 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     1021 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     1081 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     1141 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     1201 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     1261 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     1321 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     1381 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     1441 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     1501 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     1561 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     1621 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     1681 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     1741 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     1801 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     1861 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     1921 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     1981 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     2041 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     2101 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     2161 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     2221 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     2281 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     2341 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     2401 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     2461 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     2521 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     2581 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     2641 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     2701 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     2761 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     2821 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     2881 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     2941 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
     3001 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
     3061 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
     3121 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
     3181 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
     3241 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
     3301 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
     3361 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
     3421 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
     3481 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
     3541 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
     3601 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
     3661 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
     3721 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
     3781 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
     3841 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
     3901 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
     3961 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
     4021 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
     4081 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
     4141 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
     4201 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
     4261 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
     4321 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
     4381 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
     4441 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
     4501 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
     4561 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
     4621 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
     4681 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
     4741 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
     4801 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
     4861 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
     4921 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
     4981 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
     5041 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
     5101 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
     5161 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
     5221 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
     5281 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
     5341 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
     5401 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
     5461 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
     5521 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
     5581 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcaata gaatgatttg
     5641 ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat
     5701 gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat
     5761 ttttt
//
LOCUS       DACCIQ010000121         5734 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCIQ010000121 DACCIQ010000000
VERSION     DACCIQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.125"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IVM62_002544"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IVM62_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP6846458.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IVM62_002545"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IVM62_002545"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP6846459.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IVM62_002546"
     CDS             2591..3376
                     /locus_tag="IVM62_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6846460.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IVM62_002547"
     CDS             3389..4240
                     /locus_tag="IVM62_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP6846461.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IVM62_002548"
     CDS             complement(4364..4816)
                     /locus_tag="IVM62_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846462.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IVM62_002549"
     CDS             complement(5281..5649)
                     /locus_tag="IVM62_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP6846463.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1935 a          929 c         1161 g         1709 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taat
//
LOCUS       DACCIQ010000122         5690 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCIQ010000122 DACCIQ010000000
VERSION     DACCIQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.126"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(489..1298)
                     /locus_tag="IVM62_002550"
     CDS             complement(489..1298)
                     /locus_tag="IVM62_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6846464.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1298..2044)
                     /locus_tag="IVM62_002551"
     CDS             complement(1298..2044)
                     /locus_tag="IVM62_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6846465.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2062..2529)
                     /locus_tag="IVM62_002552"
     CDS             complement(2062..2529)
                     /locus_tag="IVM62_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6846466.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2529..2939)
                     /locus_tag="IVM62_002553"
     CDS             complement(2529..2939)
                     /locus_tag="IVM62_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6846467.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2950..3963)
                     /locus_tag="IVM62_002554"
     CDS             complement(2950..3963)
                     /locus_tag="IVM62_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846468.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4180..4914
                     /locus_tag="IVM62_002555"
     CDS             4180..4914
                     /locus_tag="IVM62_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6846469.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4898..5608
                     /locus_tag="IVM62_002556"
     CDS             4898..5608
                     /locus_tag="IVM62_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6846470.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1195 c          943 g         1719 t
ORIGIN      
        1 aaagcgacaa aaaccacgag caggtccatt catcctcaaa cctcggtgat cttcgcggtt
       61 taacaccatc agaagaaatg cacaaaaata ataaggagag tctgagacat aaataaattc
      121 ccagactttt agtgagtcta aatccgaata aacggtgggt cgaaagcaac tctagctgcg
      181 cgtcgatcgc agcaatctct aggtgctatg ttcggataaa tggtggtcaa aagtcagatt
      241 ttcggccatt tcgtaacagc caaccagaca aaaagcgtct gttttgtctt ctactccaat
      301 tgctcaaatc ttagcgactt ttggcttggt cttactctaa acagtttgta agcctaaact
      361 ttatttgctg tttgtttcta agatcaaagc aaaactactt tttagttggt tacttttaca
      421 agttcttttt actgttgaat aaatgtaaat cttaagaaca aatatttact tctataattc
      481 caagtttctt aaaaaattcc gccaagtgta cctaaaaagc ataaaacaaa aattcctaga
      541 ataattgtga tcgttttatg ccctttggag taaagccaga cgcataaaaa ggtaattcct
      601 aatggcagca agcccggaaa aatttgatca aaaatacttt gtaatgaagt ttcagctcca
      661 ccaaccgtga attttagtga cgtatttaaa gtaaccatcg tgcctatcat cgcgccaatt
      721 accgttaaac ctaaaatcga agcaccatga gataatttgt caatcatatg gttgtcttct
      781 gtatcatcaa taaatttagt tcctaaaaca tagccatatt ttgtcgccaa ccatttaaaa
      841 ataaaatgaa tggcagtata caaagtaaaa taaaggatca tgcctaaaat attcccttgt
      901 tgagcaaaag aaatacctat gccagccgca ataacccgaa aagttcccca gaaaaatgaa
      961 tcgccaatac ctgataaagg tcccattaat cctgttttga ttttattaat tgcgtctgta
     1021 tcaaattcag gatttttagc attttcttct tccatggtga cggacaagct cataacaaaa
     1081 ggcgcaatat gcggtgtaat gttaaatgtt tccgtatggc gctgcaacgc tgtataataa
     1141 tcatcatcgt tcggatatat tttacgtaag gaacgttcaa taccattcat ttgtccatat
     1201 gccatcattt tatcgaagga ccaagaggat tgcataaaaa aggagcgcca aaatatactt
     1261 cttaattctt ttttcccaat aacactttct tttaccatta taaatcttcc tcctcttcaa
     1321 aagttagctt ttgaacagcc agtaacgcta agcaaagtcc gacgacagcc acaccgacaa
     1381 catccatttt cagatacatt acgccgacaa atcctaagaa aagataaggc atcatcgctt
     1441 tattcgccat cattttaatt aataacgcta gcccaacaca agaaagcaca ccggccgcaa
     1501 catcaaagct gtccaataca acctgaggaa tcaacttgat catcgcattg attgtagaca
     1561 caccaaaata agtagctaca aaaactaaaa tggccgatgg aaccccaacg attaaaggtt
     1621 gagccacata atgatatttt tgtgcttttt tgaaatcatg attgactaat gcctcatcca
     1681 ttttgttgtt aaaatacgta attggcattg cataggcgaa gttacgccac atctgaagaa
     1741 agatggacaa aggtaaagtc aacgcaacgg cagttcctac tccttgaccc gattgaatcg
     1801 caattgctgt acctaaaaca ctggaagcat aaacttcagg gggcgtcgca ctccctacca
     1861 taattgagcc catgaacatg gattccaagg tggcaccaat aataattcct gactctaaat
     1921 ctcccaaaat caaaccgaca aaaggcgctg taaatagcgg tctacccatc atcgatgcac
     1981 caaatgtgta ttcatcaata gacgccaaaa aagctaccag tgcgattaat attgcttgta
     2041 tcatattctt cccctcctct tttagtcatg caaaggattg atttttttgt ccgttggtac
     2101 agcctgacat tgaactgtta tcccgttttc agtcattttt ttcaccaagt ccagttcttg
     2161 ttcattcaag taaacggaac tagtaatcat tttccgattc tcgtttttct ttaaatttcc
     2221 taaattcact tctttaatct caggtacgtg ctgagtaatt tcgtaggcat cctccaccgt
     2281 cccgacaatt aaaaatactt ggtatttctt gattttttct ccatttaaaa cagagatggc
     2341 ctctgtcata tccttcacca caaattttgt ccctgtagga acagccattt ttagtgtttt
     2401 tttgcgcaga ttattggtag cgatttcatt acttgcaacg aaaatacaat ttgcacctaa
     2461 gttatttgtc caggcaaaag caacttgccc atgaacaaaa cgttcatcta ctcgtaataa
     2521 tttaatcatt aaaaatcatc ctcttctgtt ttctctagca actcgttcac caaaactatc
     2581 ccttcattcc cgctattcat agctttttgg attagttttt tcgtgtcctg ttcacctgat
     2641 aagcaaagct ctaaaagcag cggcagattt acaccgctga ttaaataaaa attgtcttta
     2701 ccgatttgtt cggagaaagc gttagacacg cttcctccca gtaaatctac cgtcacaatt
     2761 aattcgtcat caggcgctaa gtcggaaaat agctgctcta tttcctggtt aatggagtag
     2821 tcactatcta tatacgcagt tattgctaaa agtgattttt gaggaccaat aataaattct
     2881 aaagtatcct ttaagccttt agccatcatg gaatgcgtcg caattacaat tttcctcata
     2941 gctcttacct taatattcca cagtccgata ataacgacga atatctagtg aatgatcccg
     3001 caccttttca aaatgagcac tcactcgttc taaggccgtt gccataacca ttggcgctac
     3061 atactgacgc atagcgcttg gtacacctgc taattcgaaa tccgcagtgt caaaaaccgt
     3121 cacaacatcg ctatatttat ttacaaaacg ctctacgcga tcgactagcg gacgcgtttg
     3181 atcttcacct tttaataaca caaaactcat agatttatca gtcatttcaa tagtcccatg
     3241 gaaaaattcg gctgcatgga tcgacttggt ggcaatccat tgcatttctt ccagtacgca
     3301 catagcatat tcataggcag ccccataagt attcccggct ccaacaaaca tatgaaattc
     3361 cgtattttta tgtttttctg caaattcaag tgctttctga tcattttgtt gccgaacact
     3421 tagtaatact tccggcattt tttgtaatgt ttgaataaat tcatcatatt caggaaactc
     3481 accgttattt ttcatcaggc gtgcacccaa agcaaaaaac tgtaactgaa gttcttctac
     3541 taatgtatcg ttgtcagcat cattttcaat cgcataatcc acgatttccc cgagtggtgt
     3601 agcagcattt cctacaaacg caatcgtcgt tgcaccttga ctattggcat acttaacaac
     3661 atcaacggtt tcttgagtcg taccggttaa tgaagatgtg attaacactg tatctttatt
     3721 aatctttttc ggctgtgaac aaaccacctc tgcggctatt tcatgataat aatcaagctc
     3781 tgaatgagtg cttaacagat aagctagcgg gctgattgtg gcatatgttc caccagagcc
     3841 taccaaataa agatttttaa atcccttctc actgattgca tctgccacct gattgattgt
     3901 ctcgatattt tttactgcac catttaatac tgatagaaaa cgttcttcgc taaaacctaa
     3961 catagttaaa aacctccaag tcaataattt gaaaaatata tttgcaaatg ccggcacatt
     4021 aacaaaccac ttaacctttt ttatatacca cctgttaacg tttttaatgt accacttata
     4081 gaaaacgctg tcaatagttt ttaaccactt ttatttttat ttgtaccaaa atcctttttt
     4141 catgtataat aaacaaaaat agactctgga gtgtaacgta tgaatactga agaactaacg
     4201 aacaaattaa tacaagacat tgaacaagaa aaatataaat acgcgcaagc tattccttca
     4261 gaacgtgatt ttgttcaaat ttatgaaatg aatcgttcag ccgtaaaaaa agccattcaa
     4321 caattagtaa ataaagagta cctactaaaa atacatggca aaggaacttt tgttaaaaaa
     4381 aatatccatg aacatttgca aatcaaattt catggtttgt ctgaattgtt ggaacgggaa
     4441 gggattcagc ctagcagctt aattttagct tcagaaataa aaccggctgg acacattgtc
     4501 gggaaagctt tacagctgga accaactgaa gaagtatttc atttggttcg tttaagaagc
     4561 ggcgacggtg agccaatttc tattgaagac acttatattc cattaaatct catacctgat
     4621 cttcaagctt ttgatttcca aattttttca ttttattcca cattggctgc ttatcattat
     4681 caaattgaaa atatctatca gacaatttcc agctcaaaag tgagcaataa agaagcccgt
     4741 ttgttaaaaa ccagcttaaa tcgtccggta atcagccttg ctatcactgc ggttacgaca
     4801 aatgaacaag cggttgaata cactgaagta gtggttttgc ctgaattcgc cgccatctat
     4861 actaaaaatg ttcatcaagg aaaggagatg aaaatctatg cccaaattga ttaatctgtt
     4921 ggcttatgac ctgattgaca aaattattgc ttatattcat gaacatcatt taacccccgg
     4981 aacaaaactg ccttcggaac gggacctatc agaaatgttt aatgttaatc gaactacctt
     5041 aagaggcgcg ttacaaaaac tggtggacaa cggcacctta cgggcgcaaa ccaatagcgg
     5101 ctattattta actgcgccaa aattcgaaaa aaatgcccat catttcttta caccgataga
     5161 agattctctg cttagtaagc agcggctaca gctaaaggca acggctcttt caccgaaaaa
     5221 agcagcagct ctttgtccgg aactgaatga tttcaaacag cagcaaccac aagcttattt
     5281 agaatatatt gatgcagaag caattagctt gatgttatta atccctatgg aaacattaaa
     5341 gaaaagtaat ttctcggaca atcctttcca agattttaaa agtttggcat atacgcgttc
     5401 tcaagaaatt tctttaataa ctgacaatga cgttgcggcg gacttgctgg gagttgacaa
     5461 agaagaggtt ctgttatttg tacgagagga aataagagcc ggtaaggagt tggcggcgat
     5521 tgctttaggt tatactatta gtcgccgctg caacatacag gttaatgttg ctacgacctt
     5581 gcaatccggt tctgttgcaa agttttaaat ctactatcag ataaggtaga ataatagaaa
     5641 aagatagcag gaggaatgac gatgagtcat tttaaaggaa agcaatttca
//
LOCUS       DACCIQ010000123         5476 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCIQ010000123 DACCIQ010000000
VERSION     DACCIQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.127"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            3..1481
                     /locus_tag="IVM62_002557"
     CDS             3..1481
                     /locus_tag="IVM62_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6846471.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1758..3818
                     /locus_tag="IVM62_002558"
     CDS             1758..3818
                     /locus_tag="IVM62_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846472.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            3901..4470
                     /locus_tag="IVM62_002559"
     CDS             3901..4470
                     /locus_tag="IVM62_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6846473.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4645..5451)
                     /locus_tag="IVM62_002560"
     CDS             complement(4645..5451)
                     /locus_tag="IVM62_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846474.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         1968 a          916 c          982 g         1610 t
ORIGIN      
        1 atatgaagtt ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat
       61 atttagaatt tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt
      121 tgtctaaatt taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca
      181 aaacaaagcc agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt
      241 tgacggatat caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt
      301 taacggagat cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa
      361 gcatatcaag agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa
      421 taaagttaaa gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg
      481 gggtatattt cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg
      541 aagcaaagag tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa
      601 aacgaatcaa attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac
      661 caatcggaaa acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca
      721 tcaaggattt atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga
      781 tttcttatat catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg
      841 gacttgcgga agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat
      901 tgattgatca attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa
      961 aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga
     1021 tgactagctt tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca
     1081 gtacagcaat ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac
     1141 gtttcatgat tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat
     1201 catattttga agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg
     1261 attacattat ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa
     1321 tcacttctcg tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac
     1381 agataatttg gaaaaatcca ccgactccag atgactggga atattttgga aataccatca
     1441 tcagagaaaa aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt
     1501 tgtctcacat atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa
     1561 ctagatataa ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga
     1621 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
     1681 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
     1741 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
     1801 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
     1861 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
     1921 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
     1981 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
     2041 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
     2101 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
     2161 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
     2221 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
     2281 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
     2341 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
     2401 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
     2461 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
     2521 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
     2581 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     2641 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     2701 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     2761 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     2821 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     2881 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     2941 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     3001 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     3061 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     3121 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     3181 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     3241 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     3301 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag
     3361 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     3421 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     3481 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     3541 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     3601 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     3661 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     3721 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     3781 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     3841 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     3901 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     3961 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     4021 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     4081 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     4141 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     4201 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     4261 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     4321 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     4381 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     4441 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     4501 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     4561 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     4621 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     4681 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     4741 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     4801 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     4861 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     4921 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     4981 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     5041 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     5101 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     5161 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     5221 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     5281 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     5341 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     5401 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     5461 ccttcatgta tatcct
//
LOCUS       DACCIQ010000124         5475 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCIQ010000124 DACCIQ010000000
VERSION     DACCIQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.128"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(130..216)
                     /locus_tag="IVM62_002561"
     CDS             complement(130..216)
                     /locus_tag="IVM62_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6846475.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            860..1597
                     /locus_tag="IVM62_002562"
     CDS             860..1597
                     /locus_tag="IVM62_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6846476.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            1625..2164
                     /locus_tag="IVM62_002563"
     CDS             1625..2164
                     /locus_tag="IVM62_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6846477.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            2801..3025
                     /locus_tag="IVM62_002564"
     CDS             2801..3025
                     /locus_tag="IVM62_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP6846478.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            3045..3332
                     /locus_tag="IVM62_002565"
     CDS             3045..3332
                     /locus_tag="IVM62_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6846479.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            3782..4162
                     /locus_tag="IVM62_002566"
     CDS             3782..4162
                     /locus_tag="IVM62_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6846480.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            4144..5058
                     /locus_tag="IVM62_002567"
     CDS             4144..5058
                     /locus_tag="IVM62_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6846481.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            5018..5296
                     /locus_tag="IVM62_002568"
     CDS             5018..5296
                     /locus_tag="IVM62_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846482.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
BASE COUNT         2068 a          866 c         1022 g         1519 t
ORIGIN      
        1 caaaattgaa tttgatttaa aatcatgtta aaatgaatgt gcaaatgatc aaacattaga
       61 tacacgaaag ccccagagag gtcggattct ctggggcttt ttttcaggtt gatgtcgcct
      121 ttattgattt tacttcttgt ttcggttatt caaccaataa ctgaataaag agacacaaac
      181 tcctacaaga agggggcaaa tgaacagttc aaacattaga tacacctcct tccagtcgca
      241 agactgtcgg ataggcgaca agttattata tcttaaaaaa ttaaatttgt gtggtgtttt
      301 taagtatcac ttgttttagg gttgttaggg cttgccctga ccgcctgtaa ggcgcttctc
      361 gactagatta agggaattaa tcaagaatta tcttagaacg gcttagacaa gctttcagag
      421 cctcacacct cctctttgaa caacggtttt agcaaaggag ccggaggcga ctgcgcagta
      481 taatatgaga gcatagcgat cacacctttt tagcgaccgt agggagcaag ttttccagtt
      541 tagaaaaatt cgcgaacctt tttggaggaa aaagtaggag ttttttaggc gggaaattca
      601 atgtgctttt aagatatact gttagatata ctgttagata tactgttaat aataactgtt
      661 agggattagc aaccttatgg gagagtaagt attgacatag gtagtggggg ataaattgtc
      721 acactatggg ggataaattg tcacactatg ggggataaat tgtcacacta tgggggataa
      781 attgtcacac tatgggggat aaattgtatt ttacatcctc ttatgtctat gataacataa
      841 tttattgagg tgatttttat tgagtaacga tatagttcga tataataatg gattaaattc
      901 tgtcccttta agaaaattta cccctataga tatggattta ttttggggaa tatgttctaa
      961 aatgaaaaga aaaggtacaa aagaagaaat ttttacattt aaacaaataa aagaattaat
     1021 tgattacaat ataaatgcac gtggcgaccg ttttgctaat gatttaataa aaatgtcaaa
     1081 aaaaatcatt actttgtctt ttatttatga agacgaaaaa acatatgaac aattaaatct
     1141 gtttcaaaaa tttaaaatcg ataaagtgaa tgaaacatta ttaataaagg tttctgaaga
     1201 atttgaatat atacttaatt caatcggaac taattttact cgtttcgaat tagaaaatat
     1261 gacaaactta aagagtagct acgttaaaga gttatatcgc caacttatgg ctcataaaga
     1321 cagaagatct aaaaaaggtc actggtttgt aaaaatagaa gattttagag ctctattagc
     1381 aataccagaa agttatcgca tgagagatat tgataaacaa atttttgccc aagcgaaaaa
     1441 agaattttta aacgttaatt caactcaccc tgctattttt aaaacttttg aaatcaaaaa
     1501 aattaaagcc tacaaaggaa ataagatagg atctctgcaa atttattttg aagaaaatat
     1561 accttcaatt accttagata attggttaga gaaataatac ctaacaattt aggaggcaat
     1621 tatcatgcaa cccaaagaaa cctttacaat aaaggaatta tctgaattat ttgaaataag
     1681 taggcaagca atctctaaac atgttgataa attagacccg tccttattag ctaaaaatga
     1741 gcgtggttat aaggttgtat tacgttctgg agtattgcaa cttgctagca acttaggtaa
     1801 ccaaaggtta ctatataggt tgcatgagta tgaacaagaa aacgataaaa acgtttatat
     1861 atcaagcaaa aacgtgattg taaacgagtt gctagataag ttgcgtgaag aaaatgaatt
     1921 tttacgagaa caactttccc tcaaagagca aaataattct aatatgcaaa aattactcga
     1981 ccagcaacaa cagctcaccc tacaggcaaa tcaacagatc gaaaaactgc aagaacagtt
     2041 acaattagct tatacggaag aaactccaga agatgaatca actactattc tttctgaaga
     2101 aacagaatcg atagaaaaac acacaaaaaa gaagaagcaa aaatggtggc aactttggaa
     2161 ataactggag ggaacattca tgacaagaaa aaatcttcta ttcggttcta ttttactagg
     2221 aattctagga ttcagtccga tgttattgtc agtaatcaat aaagggaata aacatggtga
     2281 attgaaattg aatccaaatt attacaacag tcctaacgaa gaattgtttg taaaagagac
     2341 ggataaataa taaaaaagac tgctcttccg agcagccagt taaaaaaata atgagagttt
     2401 attcttgaaa aagcaacgcg cttataaagc gctaccaaaa ttttatcata ttttataaaa
     2461 aataacaatc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaact
     2521 acagaaaatc aagccttaaa aagagctttt tacctagacc aattaaaaat cacaaagtga
     2581 gcgtctctat ccattggtag aggggcaact tggtctagta cacattatgt ctactagctg
     2641 tttaaataac tgatcttata attttattta aacgattatg aattcaaaac tactttttat
     2701 gacgttaatc attgttatat aataaaatgt tatataataa aattactaag attatttaat
     2761 ataacaagca attttaaaaa aacgaaaagg aaaaacaatc atgaaaaaga aagtattaaa
     2821 acattgtgtt attctaggaa tattaggaac ttgtctagct ggcatcggta caggaataaa
     2881 agttgatgca gctacttact atggaaatgg tctttattgt aacaaagaaa aatgttgggt
     2941 agattggaat caagctaaag gagaaattgg aaaaattatt gttaatggtt gggttaatca
     3001 tggtccatgg gcacctagaa ggtagtggta gggaggttat tataatggat tttactaaag
     3061 aagaaaaact tttaaatgca attagtaaag tatacaatga agcaactata gatgactatc
     3121 ctgacttaaa agaaaagctc tttctttatt ctaaagaaat cagtgaggga aaaagtgttg
     3181 gtgaagttag tatgaaatta agtagttttc ttggaagata tattttaaaa cataaatttg
     3241 gattacctaa atctttaata gaattacaag aaattgttag taaggaatct caagtatata
     3301 gaggatgggc ttctattggt atttggagtt aattctttgg aataaaatga aaatgggact
     3361 gaagaatcaa ttctgattca tcagtcccat aaattataga gccgaaaaat tatcatattt
     3421 atagatttat taatagtaaa tggcataaga atctttgaac aacgaaaaaa atgtagctct
     3481 acctaattat tgatttgcaa caatttcacc gaaggtgaaa caagcgaaac tttacaaaaa
     3541 gtggctagca tttgctagtc ttttttgtat tgtggagagt gggggggagg gggattcaag
     3601 ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacgtagg tatttggcta
     3661 gcaaatatag agcttgccaa gccctttagt gagctttagt gagctttact tattgtacct
     3721 ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa gggtgggact
     3781 tatgagcgaa caaaatcaat atttggctag caatcattct aaaaaatata cctatcgatc
     3841 cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc tgaagcagtc
     3901 agctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac aaaacgcgcg
     3961 tctggtgaca ccgaaagtgg cgccagacat tgctcaggca atcgcgcgcg atttagccaa
     4021 agctggcggg aatatcaatc aaattgccaa gtggtgcaac acacaccaac acgacgttga
     4081 tcctggcgac gcccaacgct tatccgaaaa cctaaaaatc atgcaaaagg agctacaaaa
     4141 gatatggcaa cagttaaagt aagccgaacc acttcttgta gccgagcgat caattacgcc
     4201 gaaccacgag caaccgtcaa aacagggata aactgtgaca ttgattatgc caaaagcgaa
     4261 atgaaacaaa ttcgcatgct gtatggaaaa gacgatcgtg tacaagcaca cttattgatc
     4321 cagtcttttc gaccaggaga aattaccgct gaaaaagcca atcaactagg ggcagagtat
     4381 gcgaaaaaaa tagcccctga ccaccaaatc gcgatttaca cacatacaga caaagaccat
     4441 attcataacc acattgtcat taactccgtc aatctggaaa ctgggaacaa atttcaagcc
     4501 catggtcaag catttttaaa caaatgttat gacctaaatg acgagatttg tcttgaacat
     4561 gacttaacaa tcactgaacg agggaagaaa cccgaaaaac gaaccatgtc tgaaatcaaa
     4621 ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggtttgcgat tgatcaaaca
     4681 atgaaaaaca aaaaaactcg aacgtataac caattttgtg attctcttaa aacattcggc
     4741 attcattgct tgaatcgtgg gaaaaatttt acttacgaat tgatccacaa aaagaaaaaa
     4801 gtacgatcca acaaactagg aaaggattac gaaaaggaga cgattttaca tgagctggac
     4861 agacgagaac gagcctacaa tcaacaacga accaacgagc tacgaagagc tcgagctagc
     4921 attcgcgaaa ctgcaaaaga agtacgaaga gaacaacgaa ctcttacgac tgaacaaccg
     4981 gaaattagac cgactcgaag aaaccatgac caacacgttg aaagaggagc agactaccaa
     5041 caagaattat cgttctaaaa cgttacaaga catagaaaat gaaataaaga aacaacgtgc
     5101 ttttcttgca ccttatacca aagaaagaat acaaactaca attgattcac aagtcaaaaa
     5161 attccaccat gcgctattaa accagcgaaa taaagaacag aaaaaagtaa ttatgcagcg
     5221 atcaatggat attcttgccc ccttatgttg cttaatcatg acactgttag ccttttggct
     5281 ggtatgggga gaataaatag aaatggcatt ctatttaccc tatctactca tttttgccag
     5341 tattttagga attatttggc ttagctataa ggcttatcaa acaagatatt cattaaaagg
     5401 ctctaaaatt cgatttaaga ggtttttatt gccaggggtt atatttacat tatcaatcgt
     5461 tttaagtggt ttatt
//
LOCUS       DACCIQ010000125         5329 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCIQ010000125 DACCIQ010000000
VERSION     DACCIQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.129"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(1..330)
                     /locus_tag="IVM62_002569"
     CDS             complement(1..330)
                     /locus_tag="IVM62_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP6846483.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="IVM62_002570"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="IVM62_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP6846484.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="IVM62_002571"
     CDS             1251..2264
                     /locus_tag="IVM62_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6846485.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="IVM62_002572"
     CDS             2264..3541
                     /locus_tag="IVM62_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6846486.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..>5329
                     /locus_tag="IVM62_002573"
     CDS             3604..>5329
                     /locus_tag="IVM62_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846487.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGK"
BASE COUNT         1775 a          916 c         1138 g         1500 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaag
//
LOCUS       DACCIQ010000126         5244 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCIQ010000126 DACCIQ010000000
VERSION     DACCIQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.130"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(1273..1938)
                     /locus_tag="IVM62_002574"
     CDS             complement(1273..1938)
                     /locus_tag="IVM62_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846488.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVM62_002575"
     CDS             2064..4100
                     /locus_tag="IVM62_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP6846489.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVM62_002576"
     CDS             4104..4742
                     /locus_tag="IVM62_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6846490.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVM62_002577"
     CDS             4770..5198
                     /locus_tag="IVM62_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846491.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1730 a          802 c          958 g         1754 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acag
//
LOCUS       DACCIQ010000127         4997 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCIQ010000127 DACCIQ010000000
VERSION     DACCIQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.131"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..182)
                     /locus_tag="IVM62_002578"
     CDS             complement(<1..182)
                     /locus_tag="IVM62_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6846492.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IVM62_002579"
     CDS             517..951
                     /locus_tag="IVM62_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6846493.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IVM62_002580"
     CDS             974..1294
                     /locus_tag="IVM62_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846494.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IVM62_002581"
     CDS             1435..1626
                     /locus_tag="IVM62_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846495.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IVM62_002582"
     CDS             complement(1697..1954)
                     /locus_tag="IVM62_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP6846496.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IVM62_002583"
     CDS             2112..2375
                     /locus_tag="IVM62_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846497.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IVM62_002584"
     CDS             2399..2587
                     /locus_tag="IVM62_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846498.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IVM62_002585"
     CDS             complement(2670..2855)
                     /locus_tag="IVM62_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846499.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IVM62_002586"
     CDS             3070..3441
                     /locus_tag="IVM62_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6846500.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IVM62_002587"
     tRNA            complement(3591..3663)
                     /locus_tag="IVM62_002587"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IVM62_002588"
     CDS             complement(3709..4137)
                     /locus_tag="IVM62_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846501.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IVM62_002589"
     CDS             complement(4197..4955)
                     /locus_tag="IVM62_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP6846502.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1742 a          784 c          977 g         1494 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaa
//
LOCUS       DACCIQ010000128         4906 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCIQ010000128 DACCIQ010000000
VERSION     DACCIQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.132"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVM62_002590"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVM62_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP6846503.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IVM62_002591"
     CDS             complement(2066..3064)
                     /locus_tag="IVM62_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP6846504.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IVM62_002592"
     CDS             complement(3290..3943)
                     /locus_tag="IVM62_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP6846505.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVM62_002593"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVM62_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP6846506.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1452 a         1021 c          753 g         1680 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacata
//
LOCUS       DACCIQ010000129         4830 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCIQ010000129 DACCIQ010000000
VERSION     DACCIQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.133"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(34..492)
                     /locus_tag="IVM62_002594"
     CDS             complement(34..492)
                     /locus_tag="IVM62_002594"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP6846507.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(663..1808)
                     /locus_tag="IVM62_002595"
     CDS             complement(663..1808)
                     /locus_tag="IVM62_002595"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP6846508.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1821..2738)
                     /locus_tag="IVM62_002596"
     CDS             complement(1821..2738)
                     /locus_tag="IVM62_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP6846509.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3166..3729
                     /gene="efp"
                     /locus_tag="IVM62_002597"
     CDS             3166..3729
                     /gene="efp"
                     /locus_tag="IVM62_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP6846510.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(3869..>4830)
                     /locus_tag="IVM62_002598"
     CDS             complement(3869..>4830)
                     /locus_tag="IVM62_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6846511.1"
                     /translation="RIGTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGV
                     STRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKV
                     REENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVI
                     SDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINL
                     AREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEV
                     RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         1445 a          920 c          927 g         1538 t
ORIGIN      
        1 atattgcttc tcatctttac gttcttttag aaactatgct tctacttcag accattcatc
       61 gcgttttgct aaaaattcac gagccaaagc ttgtgcacct tctagtgtat ggctggcagc
      121 ccagccacat tgtgtttcat tgcttgcagg tacttcagta gcttcaagaa catctttcat
      181 tgttgcttca agaacatcta aaatgtcttc gtagctttca tgattcaata cagtcaagta
      241 aaagcctgtt tggcatccca ttggagacca gtctacgatg taatctgcgt gattgcggat
      301 tagttcggca gtcaagtgct caagtgaatg tagacttggc atgtccatgt gttctttatt
      361 tggttgttta aagcggacgt cgtatttaat gatgacatcg ccatgcgcac ccgtttttcg
      421 atcagctaca cgtacataag gtgctttgac ttttgtatgg tcaagattaa aactttcgac
      481 attcattctc atttgtaaga ctcctatctg tttgttcatt cgttttgatg cttttcacta
      541 aagaattatt tagtgaaatt tacaaaaacg tctttctact tgattctaac acttatcgtt
      601 tccaaggtgt agcaatctga aatatttttt agatttttct gctaagtcgt ttattgggac
      661 ttctagttct tgattctgtc aaatgcctga gctaaatcgt ttatgagatc ctgaccatac
      721 tcaattccta cagaaagacg gatcaagccg tcctttatgc cagcctcttc tcgtttttct
      781 tttggaatag acgcatgagt catgacagaa ggaatttcca ctaaactttc tacgccacct
      841 aaactttctg ctaaagtaaa tagttggagt gattcaacaa atggaatagc agattcttca
      901 ttttttaaag taaaagaaat cattccgcta aagccgctca tctgctgctt agcaactcca
      961 tggagttcat gatcgggtaa tcctggatag tagacctttt ctactgctgg atgagaaaat
     1021 agaaaatcag ctaccacaaa agcatttttt tgatgttcct ccatacgaac acttagtgtc
     1081 ttgattccgc gctgaagtaa ccagctatct tggggaccaa gtacggctcc gatagcattt
     1141 tggtaaaatc cgatctgatc ggctagatct ttatggttag aagtcactaa accagctact
     1201 acatcgctgt gtccgcctag atatttggta ccgctatgga caacgatatc agaaccgaga
     1261 tctaaaggtc tttgaaaata aggagtagca aatgtattgt cagcaataac tatcaatcca
     1321 tgttgatgag ccagagtggc acttttttct aaatcagtaa tctttagaag cggattgcta
     1381 ggtgtttcaa gataaagtgc ttttgtattg ggtttgatgg attgttctat tttatcaaga
     1441 ttactcgtat ctatgatcgt gtactcgaga ccatttttag taagaacttt gtcaaataga
     1501 cggaatgttc ctccatagac atcatctcct aataagatat gatcgccagc ttgaaaaaga
     1561 gaaaaaacgg catgaatccc agaaagacca gaagaaaaag cgaatcctcg aacaccgcct
     1621 tccaaatcag caatcagttc ttccaatgca aaacgagtag gattaccaga tcttgaatat
     1681 tcatattgtt taggctggcc gacgccatct tgccgataag tggatgtttg ataaataggt
     1741 acactgacag ctcctgttgt gggatcttca ctgatgcctc cgtggatcaa ttttgtttga
     1801 atgtgcatgt ttctttcctc ctataagtaa atgttttttg ataaatagcg gtctgctgcg
     1861 tctggaaaaa tcgtaacgac acgatgacca ggtggtaaac gacgaatttc tcttaatgct
     1921 gctgcaaaag ccgcaccact cgaacttcca actaatagtc cttgttctcg tgctaactgc
     1981 ctagtatagt taaacccttc tacatcagag attgtctcga tctgattgat tgaaagggga
     2041 gaaagaaacg gcggaatgaa ttctacaccg ataccctcaa tttcgtgagg ggctggatca
     2101 ccaccattta acacagagcc ctctggttct acaccgatta ttcgaatgtc gggatatttt
     2161 tctttcaaaa aggtagaagt tccagcaaag gttccaccac tcccaattcc tgctacaaaa
     2221 ctatggatat tttctttgat ttcatgaaag atttctggcc ctaacgtatg atagtaagca
     2281 gcaggattat ctttattttc aaattgaaga ggcagatagc tgtttgatat ctcctctgca
     2341 agttttttac ttttgctgat ggcaccgatg attccttgct cactaggaga atggatgatc
     2401 tttgctccca atgctttcat caattgttgt ttttctaagc taaacttctc tggaacaaca
     2461 aaaaccgttc ttaatttgta tgtgagtgcg gccagagcga tcccaattcc tgtatttccg
     2521 gcagttggtt cgatgatcgt cgttttttca ttgatttttc cttgggcaaa tccttctttg
     2581 atcaaataac tgcccaatct atctttgata cttcctccag gatttaaata ttctagtttt
     2641 gcataaatag cagaaccttt aggcatagaa tagtgctgat tgtcaaagcg ataaagcggt
     2701 gtggaaccaa tagcttctgt aattgtcgag ataatcatgt gggcctccta aaaaaagata
     2761 ataaaaaaag gagctgacta ttaaatcagc tccttgaaag acatagagaa aaattgagta
     2821 gataaaaaga taagttacct ggtactcatt ctatgaaggg caattaatag acatgacaaa
     2881 tagtatgctg cttttatgca ccatacaaca gcaacgattc aattgttttt ggttgctgac
     2941 tgtcatgtgt atccctccaa gtaaaatttt tattaacgat agcatgaaaa aaaataaaag
     3001 tcaaaggctt gaaagaaaaa aatatttcga tagcatcgtt tacccgtgtt tatttttctg
     3061 acaaataggc atgagacctt aaaagataga gaatactcta gttttctggc cggttgtctg
     3121 atacaataaa gttgattatg ataaagaaaa gaaggagcaa aaataatgat ctcagcaagt
     3181 gatttaagag ccggtatgac tttcgtacaa gacggcaaac tgattaaagt tctggatgca
     3241 agccaccata aacctggtaa aggaaataca gttatgcgta tgaaactgaa agatgtccgt
     3301 tcaggtgcaa catacgacac aacgttcaga ccagaagaaa aatttgaaaa agcacatatc
     3361 gacacaaaac ctgtacaata tttgtacacg atggatgata cagctttctt catggatttg
     3421 gaaacatatg agcaatatga aattcctgta gaaacagtag aagaagaaat gaaatatatc
     3481 ctagaaaaca tggaagtaaa aatccaattt ttcggtagcg aagtcatcgg cgttcaatta
     3541 ccaacaactg tcgtattacg tgttgtggaa acacaacctt ccatcaaagg tgcaacagtc
     3601 actggttcag gtaagccagc gacaatggaa actggcttag tagtcaatgt tcctgacttc
     3661 atcgaaacag atgaattgtt ggaaatcaat acagcagaag gttcttatgt aaaacgtgcg
     3721 tcaaaataat tcgctaacgg attgataaga atttgaaaac agagcttgac ctcttgaaag
     3781 gcagatcatc ggatctgcct ttcaagaggt tttttgtagt caagtccaca atcctgtgta
     3841 aaatactata caatgctctc actatttttt acttggcaaa tttaatatat tttcttgatg
     3901 aagaaagcca ctcatcgtgt tgatcaataa ggactgctcc gattaaacga ttggctgaaa
     3961 cgcagtttgg aaagatacga ataatttttt ctcttctgcg cacttcttga ttcagtcgtt
     4021 caattagatt ggtactcttt agtcgattgt gggaatttcc ttgtacggta tattgaaagg
     4081 cgtcttcgaa tccatcatcc aatgatgcgc aagcttttga atattttggt tgatcgatat
     4141 aatcatgaat caatcgattt ttagcctcac gagctaagtt aatatctgtg aacttaaaaa
     4201 ttcctttaac agcttctctg aaagattttg aatttttttt aggaatggtg gtaaagatat
     4261 ttcttaggaa gtgaacttgg catctttgcc aacttgcgtt ggtgaaggat tttctaatgg
     4321 cagagactaa tcctttgtgc gcatcagaaa taacgagttc tgtaccttgt aaaccgcgtt
     4381 cttttaggta ttcaaaaaat gttatccagg tctcttcgct ttcgccactt tgaatcatga
     4441 agccgataat ttcacggtcg ccatctttgg ttattccaat cgctatatga cagctttttg
     4501 agagtactcg attttcttct cgtactttta tatagagtac atcggtcatt aagtaaggat
     4561 aatttttttc tgataataaa cgattctgcc actcgttaac cattggttct agctgttctg
     4621 ttaagctaga aacgaaggac ttagagacgg atttaccaca aagttcttcc acaatttttg
     4681 atactttacg agttgaaacg cctgatacat acatttccaa cattgaagca atgagggctt
     4741 tttcgtttcg ttgataacgt tcaaacactg tgggtgaaaa atggccatca cgtgttctgg
     4801 gtacttttaa ttctagcgtg cctatacgtg
//
LOCUS       DACCIQ010000130         4713 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCIQ010000130 DACCIQ010000000
VERSION     DACCIQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.134"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            108..359
                     /locus_tag="IVM62_002599"
                     /pseudo
     CDS             108..359
                     /locus_tag="IVM62_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="IVM62_002600"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="IVM62_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6846512.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1767
                     /locus_tag="IVM62_002601"
                     /pseudo
     CDS             1656..1767
                     /locus_tag="IVM62_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="IVM62_002602"
     CDS             1788..1871
                     /locus_tag="IVM62_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP6846513.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="IVM62_002603"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="IVM62_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6846514.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="IVM62_002604"
     CDS             complement(2996..3859)
                     /locus_tag="IVM62_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP6846515.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="IVM62_002605"
     CDS             complement(3861..4133)
                     /locus_tag="IVM62_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6846516.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="IVM62_002606"
     CDS             complement(4150..4365)
                     /locus_tag="IVM62_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP6846517.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4713)
                     /locus_tag="IVM62_002607"
     CDS             complement(4457..>4713)
                     /locus_tag="IVM62_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP6846518.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1605 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tca
//
LOCUS       DACCIQ010000131         4606 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCIQ010000131 DACCIQ010000000
VERSION     DACCIQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.135"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..774)
                     /locus_tag="IVM62_002608"
     CDS             complement(<1..774)
                     /locus_tag="IVM62_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846519.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
     gene            982..1956
                     /locus_tag="IVM62_002609"
     CDS             982..1956
                     /locus_tag="IVM62_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846520.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1938..2399
                     /locus_tag="IVM62_002610"
     CDS             1938..2399
                     /locus_tag="IVM62_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6846521.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            2449..2913
                     /locus_tag="IVM62_002611"
     CDS             2449..2913
                     /locus_tag="IVM62_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6846522.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2927..3676
                     /locus_tag="IVM62_002612"
     CDS             2927..3676
                     /locus_tag="IVM62_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6846523.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            3676..4506
                     /locus_tag="IVM62_002613"
     CDS             3676..4506
                     /locus_tag="IVM62_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6846524.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1516 a          655 c          880 g         1555 t
ORIGIN      
        1 actgtcatga atatttccag gagtgatgat tgtgctaaga ataaatccat tgtcgtcgca
       61 tgaagtatgg gcagaataag caaattgttt ttcttgttcg cctttgacgt agtagccgct
      121 ttccggatca gctttactta tttttctttc tttcagcttc acttcctttt ctaagtccaa
      181 agtaaaaggg cgttttccag cctctattcg ttgacgattg atctcatctt ctaattcatc
      241 ctgataggct ttcgcttcat catgggtcat ttctttcgta aacttatgtt tattggcgtt
      301 agctttgatg tgggtagaat ctagatgaaa attatcctct gttacaaaac ctgctttaac
      361 tgcttgttct aaaatatagg caaaaatatc ctcaaacacc gtcgtttctc taaatcggcg
      421 aacataattc tttccaaaag ttgagaaatg ggggattttc tcttcgaagg agtagcctaa
      481 aaaccagcgg taagcgacat tggtatcgac ttccttaatg gtttgtcgca tggaacggat
      541 accgaataaa tactgaataa acaccaattt gatcagaatc actgggtcga tacttgggcg
      601 acctaaatcg gagtaagtgg cttcgacgat tggataaata aattcaaaat caagcgcggc
      661 atcaatcttt cgaacgagat gatcttgagg aactagatca tcaatcgtaa gcatcgcaaa
      721 ttgatttcta ccattcgtat tgtttttaga catcatagct atcactcctt gcattcgttt
      781 tcataaatct attataaaag aaaagactgt agacatcatc cataaaatgg actttgtcta
      841 cagtctggca ttttattata taaaattgtt ataaaaaaat ttttttgaaa aatagtattg
      901 caaaataaag caatttcata gataataggt attgtaaagt tatatgactt tataatactt
      961 attagagtga gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg
     1021 gcagcgttaa aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa
     1081 gccgttttaa tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt
     1141 tatactgcta acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt
     1201 atcacttgtt cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga
     1261 gaatttggag caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca
     1321 gctgaatatc ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa
     1381 ggaattcttt atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg
     1441 gaaacttgct tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa
     1501 tttgaaataa ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca
     1561 attggtagcg gtatcaatta cggagaaatt tactccacag cagcttgctg gttcatggaa
     1621 atgcaatgga ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa
     1681 atcactgatt atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta
     1741 gatcaacggg tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa
     1801 gctgattttg acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt
     1861 agtggcgttg ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta
     1921 gatgttagac gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg
     1981 caaatcaatt atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa
     2041 aggcaacaaa attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag
     2101 aaaatactag tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt
     2161 tgcaacctct aattatttta acggatttaa ttggaggaac acctaataat gtttctatta
     2221 aacagttgaa caacaataga attcaaatta ttgctgggat taatttacct cttttactgg
     2281 agttattaat ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac
     2341 tgaataactc tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag
     2401 gtggcaattt ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta
     2461 gtgcgtgtag accacagatt acttcatgga caagtaatat tttcttggac taaacagtat
     2521 gatattaacc atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg
     2581 gctttgacaa ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt
     2641 aaagggatta tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag
     2701 gaagcttttg aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg
     2761 tctaagaaaa gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta
     2821 gaagccacaa aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct
     2881 tcaagtattg aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc
     2941 cattttatta ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca
     3001 aaatattggt agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca
     3061 tacagggatt gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg
     3121 ggcggctgga cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt
     3181 aactaatagt agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga
     3241 atttatgagt attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc
     3301 cattgaaaat aataaattta gaaaagctca aattattcat atttattggt catttatttt
     3361 taatgctttt gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt
     3421 ggcagattta gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa
     3481 tatgctttcc gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata
     3541 tccttacttt atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt
     3601 gacaattttt gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga
     3661 agaggcgcat ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat
     3721 cttaataaga tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa
     3781 atgcatatgg gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat
     3841 gaagagaaaa aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa
     3901 atgacttcat ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa
     3961 gaaggaaact ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt
     4021 gctggaattg gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga
     4081 ttgtcttttg ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct
     4141 ataccttcta ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa
     4201 taccttacta aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc
     4261 attggtttgg ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta
     4321 aaattcacta cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct
     4381 ggattattac cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt
     4441 acaaatatgc tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc
     4501 ttataatgat taaggctcta atttttgata tggacggagt tttaattgat agcgagatta
     4561 tgtatcaaaa gtgggtattt aattttttaa aacaagaaaa tttaag
//
LOCUS       DACCIQ010000132         4568 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCIQ010000132 DACCIQ010000000
VERSION     DACCIQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.136"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(10..972)
                     /locus_tag="IVM62_002614"
     CDS             complement(10..972)
                     /locus_tag="IVM62_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6846525.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(974..2413)
                     /locus_tag="IVM62_002615"
     CDS             complement(974..2413)
                     /locus_tag="IVM62_002615"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP6846526.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2598..4544
                     /locus_tag="IVM62_002616"
     CDS             2598..4544
                     /locus_tag="IVM62_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP6846527.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1442 a          785 c          762 g         1579 t
ORIGIN      
        1 aatttaacgt taaatacttc ctccagaaag tattgagatt ggtaaaatat aatccttatt
       61 tgtcttttcg cctttaattt ttttcagaag aatatcaaca ataagacttg caatttcatc
      121 aattggttgt cttattgttg tcaattgtgg gaaaaatttc tcaataaaag aggtaccatc
      181 gtatccaata atttttaaat cttctgggat attaagttta agttgatgag caattttcat
      241 agtgagaatg gctgttaaat catcagaaac aaaaatacca tctggtttgt gatttataat
      301 agttgacttt atttccattt cacgacggat agtagacaga ttattaggaa ttttgaaaat
      361 ttttccattt ggattttgaa aagaaaaacc tagtgcacgt aattctgtgg gagaatccgt
      421 gttatcattt cctgtaatca taatggtatt ttgacaacca tttttttgta aagtcttagc
      481 tgcaagtttt cccccttcaa agttatctga agaaacaatg gaaacattag gagctaaatt
      541 tctatcaaaa gcaacaatag gtgctctcac acgctcataa tcagcaattc caagattatg
      601 gcttgatgag attatgccat caacttggtt ggcttcaagc atttcaatgt attctctctc
      661 tttagcagga tcattttgac tattgcaaat aatcgtttta tatccatgtt taaataactc
      721 tatttcgaga tactcaatga gttcagagta aaagatatta ctaatattag gaaaaataag
      781 accaataagc tgaggtgact ttccttgaag tccacgtgct aaattatttg gtttataacc
      841 aagagttttc atagcttcat gaactttttg ttttgttttt tctgataaat atcccttatt
      901 attaatgacg cgtgaaacgg ttgttgggct aactccagct aattcagcga catcagttaa
      961 ttttgctacc attttaatat tttaattcaa agtattttcc agtaggttcg cctgatttga
     1021 tttgaatacc attttggtga ttttcaggga atacacgact agtaaacact ttttctcctt
     1081 tattaataaa aatctcaatg attgagttat ctacaaaaat gttagcagtt gtttctttag
     1141 tatcaattga gcattcacga atcgtaccaa agtcagtggc atattgaatg cctgctttgc
     1201 tgcgatctaa aataattttt ccttctttgg tatctacaat caagctaagt ccatttcctt
     1261 tactatcaga gaaaagaagt aactccgatt tctgatttgg aggaaaagtt agttcaagct
     1321 cataagtatt atttgtttct gcttttgctg taaattgttc agaactagca cgtaatgatg
     1381 taatagcttc tactggatat tggtaaagtt taccatcttt tattgaaagt tcttttacaa
     1441 gactcattgc tccctgataa tcatacttat cagttggata atcgatatca ggtagtccaa
     1501 tccaactgac aatcagagca cgaccatcag gagtattgaa tccttgagtc gcatacgctt
     1561 caaatccata atcaagattt tgaatttctg aagcgcccac aagttttact ttttctatgt
     1621 caagcgattg acagatctta aaagtattcg gatagatatt cccataattt agctcagatt
     1681 tatccaaccc ttgaggacaa taaatgagaa caggtttttt gtctacaaat actagatttg
     1741 gacactcaat catgtattct gaaccagtgc cgccaaaatc aaggttgcca acttcctccc
     1801 atgtttccac gttattgtca tccgctttgt aaagtttgat aaagcctgat ttttctaaat
     1861 tttgtgctcc aacaatcgca tacaattgtc cttcataatt aaacatttgt ggatcacgaa
     1921 aatgatcagt tacatcagca ggtttatgaa taagaacatt ctcacctttc gtaatgttgt
     1981 agtctttatc catccatgct ccgatttgaa gtgtatcacg ttcccaattt tcatttcgaa
     2041 cgtttcctgt atagagtaag aatagattcc cctctatttc atatgcagaa ccggaataag
     2101 cgccgtggct atctcgtttt gtgtctggaa gaagttctgc tcctgttaca tgaaaatgaa
     2161 ctaaatcatc tgattcattg tgtacccatt ctttcaaccc atgagcagct ccaaatggcc
     2221 aattttgata aaaaagcgta tattttccgt taaaaaaaga aaaaccatta ggatcgttta
     2281 acaatcctgt tttaggttcg atatgatagc tagcacgcca aggtgatcgt tttacattat
     2341 ccttaatttt atccatctca tctttagtcc aatcagaata agcacgatat cgtatttctc
     2401 gaggtaagtt catattttac tccttcaatt tatcttttta caactagtat ggtaaacgtt
     2461 ttccgttgaa aaatcaatca tttcatttaa aaaaataaac tatatgacaa acgtttgaca
     2521 tatttttaag atatgctaaa ataaatttgc atttcagtga accgttttca cgtaaaaata
     2581 aaaaaggagt gattcgaatg aattacagta aagtagcttc cgacgtaata caagcggtcg
     2641 gaaaagataa ccttattgca gctgcacact gcgctacacg tcttcgtctc gtactaaaag
     2701 atgacacgaa agtgaatcaa aaagctttag acgaaaatcc agatgtgaaa ggcacattta
     2761 aaattgatgg acaatatcaa gtgattatcg gtgcaggtga tgttaatttc gtttatgacg
     2821 aacttatcaa aaaaactgga ctttcggaaa tttcaacaga tgatttaaaa caaattgcta
     2881 gtaaagataa aaaattcaac cccattatgg cattaattaa attattatca gatatttttg
     2941 ttccaattat acctgccctt gttgcaggtg gtttattaat ggcattacaa aactttttaa
     3001 cagctgctgg actttttgga ccaaaatcaa ttgaagaaat gtatcctgcg attgaaggta
     3061 tttcctcaat gatccaactg atgtccgctg caccattcat gttcttacca attttagtag
     3121 gtatttcagc tgctaaacgt tttggagcaa accaatttct tggtgctgca attggtatga
     3181 tcatgacaac accagatctt ggtggctcaa ctgaatattg gaatattttt ggttatcatg
     3241 tttcacaaac aaactatgct tatcaagtta ttccagtttt agcagctgtt tggatattat
     3301 ctatattaga aaaattcttt cataaaaaat tgccatcatc tattgacttt acattcacgc
     3361 cattattatc agtaatgatt actggatttt tgactttcac agttattggc cctgtaatgt
     3421 tattgttgtc taatggaatt acagacatta ttgtttggct atataacact actggattta
     3481 ttggaatggg tatttttgga ggaacatatt cacttatagt catgactggt ttacatcaat
     3541 catttccagc tattgaaact caattacttt cagcgtggac aaatggtatc ggacatggag
     3601 actttatctt tgtagttgca tctatggcaa acgttgctca aggtgctgca acatttgcca
     3661 tttggttctt aactaaaaat tctaagacaa aaagtcttgc ttcctctgcg ggtgtctcag
     3721 cacttcttgg aattacagag cctgccttgt tcggagttaa cttaaaatac cgctttccat
     3781 tcttttgtgc tttaattggt tcaggaattg ctacagcagt agcaggatta cttaaagtag
     3841 ttgctgtttc acttggttca gcaggtttct tgggatttct ttcaattaat gcaacatcta
     3901 ttccatttta cgtattgtgt gaattgatta gctttgtagt tgcttttgca attacttact
     3961 tctacggtaa aacaaaagct gctggtatct ttaaagcaga agaaattgct gaacaaacac
     4021 cagttaattc ttcaaaagta gcaacaacag atccaatatc tactgatgtt gaatcaacac
     4081 aaacaaaagt ttcatcagaa acaattgtta gtcccatttc tggtaaagtg attagtcttt
     4141 cttcagtaaa tgatccagtc ttttcatcag aagctatggg aaaagggatt gccattaaac
     4201 caaatggaaa cacggtccat tctccagtag atggtactat tcaactcacg tttgaaactg
     4261 gacatgctta tggtattaaa tcaaataatg gtgctgaagt tctaattcat attggtattg
     4321 atactgtatc aatgggagga aaaggattta cacaaaaagt tactactaat caaaaagtta
     4381 gaaaaggcga tattcttgga gcatttgacc gttcaatgat tgagaaagaa ggtcttgatg
     4441 atacaacaat gattattgtg accaatactt cggattattc agaagtaaat atagtagctg
     4501 aaaatacagt cactgagggt actgatttac ttgaaatcaa ataaatatat ttccctgagt
     4561 aaagatgg
//
LOCUS       DACCIQ010000133         4494 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCIQ010000133 DACCIQ010000000
VERSION     DACCIQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.137"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..398
                     /locus_tag="IVM62_002617"
     CDS             <1..398
                     /locus_tag="IVM62_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846528.1"
                     /translation="NTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQMEKL
                     KTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRRE
                     KLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            410..679
                     /locus_tag="IVM62_002618"
     CDS             410..679
                     /locus_tag="IVM62_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846529.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            841..1143
                     /locus_tag="IVM62_002619"
     CDS             841..1143
                     /locus_tag="IVM62_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846530.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1140..1301
                     /locus_tag="IVM62_002620"
     CDS             1140..1301
                     /locus_tag="IVM62_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6846531.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1298..1603
                     /locus_tag="IVM62_002621"
     CDS             1298..1603
                     /locus_tag="IVM62_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846532.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1603..1959
                     /locus_tag="IVM62_002622"
     CDS             1603..1959
                     /locus_tag="IVM62_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP6846533.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1919..2164
                     /locus_tag="IVM62_002623"
     CDS             1919..2164
                     /locus_tag="IVM62_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846534.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2161..2580
                     /locus_tag="IVM62_002624"
     CDS             2161..2580
                     /locus_tag="IVM62_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP6846535.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            2577..3134
                     /locus_tag="IVM62_002625"
     CDS             2577..3134
                     /locus_tag="IVM62_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP6846536.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3131..3427
                     /locus_tag="IVM62_002626"
     CDS             3131..3427
                     /locus_tag="IVM62_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846537.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3504..3917
                     /locus_tag="IVM62_002627"
     CDS             3504..3917
                     /locus_tag="IVM62_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6846538.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT         1651 a          690 c         1030 g         1123 t
ORIGIN      
        1 ataatacatt taataataca aaagaatata taagagagtt accgccttcg aaaaaatcga
       61 aggctaagcc cgtccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc
      121 agatggagaa actcaaaaca gaattcccta atgattacca agagcgaatc gaacgactgt
      181 cagagtattg tgaatcatct ggtaagactt ataaaaacta tttggcaact attcgaagtt
      241 gggcaaggaa agaaaaaagt gaacctaaga acgcaagcag tggatacaag cgcacaggaa
      301 gacgagagaa gcttcctgaa tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc
      361 tagaacgagc taatagacaa tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga
      421 ttatctagaa ctaattaatg aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct
      481 gagagacgta tatgatcaac tcgaagaagc tggaattgaa ggaattaaga atgatcgttc
      541 gagttggagt aagctcagat actatttcgc actttatatc gatacaacac aattaagaaa
      601 tttagcatat accaaattac tatttgttga ttgtgttaaa ggcttgcaaa aacatcttaa
      661 tgaacttgag caggtgtaat cagatggacc taaagacatt tacagcacag attgaactaa
      721 tgcatcaaga agctttaaga caaagtgtat cgtacgaaga caagtggctc aacacgttcc
      781 atggcggacg tgagagcgca cttgatcaag tactcaaatt actgaaagga gaatgtcggg
      841 atggataaga aagcggcaat gcagcgaatt atcgaattga cttattcaga agattggcaa
      901 aatgacaaag aagctgcttc agaagtgatg aggcttggaa gagcgatgtg ggcagacaag
      961 agcaacaagc caagaccacg aaaaatcgca atttggcacg gtgacaaact tctagtgata
     1021 gggacagctg aacagttagc aagtctcaca ggcttgcacg agaaaatcgt gaggaaaaga
     1081 gcaaggtgtg gctacacaga cgctaagaag agaacgttta gatacgtgga ggaatcatca
     1141 tgacaacaga agaagtgatt caaatgcgta ttcgaagcct tcagcgtgag attgacgatc
     1201 tggaacgaac aaaggcagtg atggtcaatg aaacggcgag aaaggcaatc gatttgcaca
     1261 tagagaattt aagaagggaa atccatcgat tggaggaatg agcgtggata agaaagcagc
     1321 aatgaaaaga attgctgaat taaccaagtc agaatcttgg caagaagaca aagaaatagt
     1381 tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc
     1441 gagaaaaatt gcaatctggc atgatgaccg aattttagta acaggtactg ctgaacagtt
     1501 atctgaaatt actggattaa gcaaaaacat tatctgggat agagctagga gcttatggat
     1561 tgattcaaaa ggacgacagt ttaggtatgt ggaggagaga taatggatct cattacacaa
     1621 tacagtgaca tcatcctcaa gaaaatcatg atgaagattc agaaagataa aaaatcaaaa
     1681 gaacgagctg aattagttaa gttagaaatg gctgaaacag gagcaggagt gcgaagtagc
     1741 aggcattgga aagcagcagc aaacattgaa ttttattaca acgaaattca aaaagggttc
     1801 gatcagatgc gtgagctgga tcggcaaaca aattggagca agaaacttca tcaagatcgt
     1861 ttcaaatttg tagagaagta taaagaaata ttagaagagt atttgaggag gacagcaaat
     1921 gataaaaaaa ctcgttcaat tcagcatgga tttatatgat atcgaatcag gagcaacact
     1981 atctgtggaa tcggaccatc taatcataaa ttttggtgga aagcgccaga ttattttgtg
     2041 ggtagttgat gatgtactgt ttccagaaat tgttcatgat ttcgaagaat caaaagcggt
     2101 tgagtttgaa atagtgaaaa aagtaatgga attgattgaa aaatacgagg aggacagcga
     2161 atgataccga agtttagagc gtgggataaa cgaaagaacg taatgagaga tgtagccgtc
     2221 ttgcatttta ctaaaaacgg caaaacaaac tttattgaat attggataaa tcctaccgaa
     2281 ttgaaatcat atcacgtgcg aaacatcgac ctcatgcaat ccacaggaat gaaagataag
     2341 aatggtgtgg atatatttga aggggatata gtattagtca gcgtgcgaaa tggcttcgat
     2401 tacttagata ataaagtctg tattgtcaaa aattcaatag attattccgg attagtttgt
     2461 gccactgttg atgaagactt agagtatcaa atttttaaca cagagctgtt tgaagaatac
     2521 acgtacgaag tcaccggaaa tatatacgag aatagcgagt tattggagga acagcgatga
     2581 ataaacagga agcaatcaaa aagttagaaa gtattaaagc gataggaaat gatgcaatag
     2641 ctgcttgcta taacgagagt ataaattcag gtattacgtt aatgaaaaaa atagacgaac
     2701 cgcagaaacc agttattcca caactcgtgg ccggttggct tgagaaatct acggaccctt
     2761 ttacaaaagc tgaaaaaata gcgtatttaa tcaaatctaa agatggtgat tcatattatt
     2821 tctgtgattg gtttgtacga gatggcatag tgacgcaaga gcaaggagaa gaattactcg
     2881 cttgggcaac aagacaatca tatgaaacac tattgagcct atacaacggc tacgaggttg
     2941 agaaagagcc gttatgggca ataaagaatg ccgatggaaa ctatcttact aaatgtgctt
     3001 tatggggaaa agatggagta aattatagtt ttgaatgcaa tccatctcat cgattgcttt
     3061 tcactgataa agcaacagcg gatgctgcag cattgttggt gaatggaaca gtggaagagg
     3121 tggtagaaag atgaaactaa aagacggatt ttacgctagc agtcacggta tcggcggttt
     3181 aatgctagat atgccgacaa agaaccctaa aacacgcaag aaatcaaaat tcaaagtcgg
     3241 tgacatggtc cgctgtgaag cagaagagtt cgtttatccg ttcagaggat acgttaaaaa
     3301 gatactgtca aactcagcaa tcattcgtat tgaaaataca atgaagtgtg atcgttcgac
     3361 tgcaaaacac aagcattatt tagctgtagc gagattagtt gacatggaag taatcaagag
     3421 caaataaaaa aagccggatc gctccgactg attcaataaa tccaacacat ttattatatc
     3481 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     3541 gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct
     3601 ctttgataga tattaagtcg ccgattataa cggatatgcc gaaggcaccg aagcatggca
     3661 ataaggcaga agacgcgatt attcagatga tggatataga aacggagaga gatgcgattc
     3721 tagcggcttt gatggctctt agtctgatta gtcgtcagat actctactac agcttctgtg
     3781 tgccagatag cttctcaaac tacagaatta gccgtgaagt gggttattca gaaagaagta
     3841 tacaacggat gaagtcggaa gctctaatag agtttgcaga agcatataaa cacggaaaaa
     3901 taattgctta taaataattt ggcggttttt tggcggaatg atggcggttt ttagacattt
     3961 accagtgtta ttatggtagt gtcgaaagat taggaaacag gatcgacaaa ataaacgaag
     4021 ggaggcaatc tccctcatcg ttttaaactt cttcttagat agacagcaac taattatgaa
     4081 gaaaaggatg tgaattcaac tcctcctaaa ttcttcatcg gttgctgttt attttgatta
     4141 gacagcagca ctcacaaaac taaacatgtt cttgtcgtct ttcaactgct gctgtctatt
     4201 aatttatgta ttggagggaa gaaaaatgaa attatcagta gaaggcactc cagaagaaat
     4261 acaagaattg ctccaagcta ttggtagtag caaggagcaa aaggtaagtg tttctaattc
     4321 agatattgac ggtatttgca attgtttaaa cagagaaaaa atatctaatc cttactaaga
     4381 gaaaatatgt tcgttaatga atttccaatt ttcctctgta gctctacctt gataattttt
     4441 ttgcagttca cagatgaata aggaatcgtt attatcaagt actgtattta attt
//
LOCUS       DACCIQ010000134         4491 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCIQ010000134 DACCIQ010000000
VERSION     DACCIQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.138"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            211..1917
                     /locus_tag="IVM62_002628"
     CDS             211..1917
                     /locus_tag="IVM62_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP6846539.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="IVM62_002629"
     CDS             complement(2075..2977)
                     /locus_tag="IVM62_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6846540.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3678)
                     /locus_tag="IVM62_002630"
     CDS             complement(2974..3678)
                     /locus_tag="IVM62_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP6846541.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="IVM62_002631"
     CDS             complement(3709..4350)
                     /locus_tag="IVM62_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6846542.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1388 a          956 c          774 g         1373 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaaa aatagagaaa atctatatat aatagaaaaa a
//
LOCUS       DACCIQ010000135         4446 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCIQ010000135 DACCIQ010000000
VERSION     DACCIQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.139"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            712..1680
                     /locus_tag="IVM62_002632"
     CDS             712..1680
                     /locus_tag="IVM62_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846543.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1691..2506
                     /locus_tag="IVM62_002633"
     CDS             1691..2506
                     /locus_tag="IVM62_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP6846544.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2574..3283
                     /locus_tag="IVM62_002634"
                     /pseudo
     CDS             2574..3283
                     /locus_tag="IVM62_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            3319..>4446
                     /locus_tag="IVM62_002635"
     CDS             3319..>4446
                     /locus_tag="IVM62_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP6846545.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1498 a          664 c          920 g         1364 t
ORIGIN      
        1 cataaaaaaa ttcatctttt ttagtttaat aattcaccaa aaaggtgagt aatgccattt
       61 ttgaaaattg agcttgtttt atgtctacat ctgtatccgt atataatcca aaggattata
      121 aactcagaag agtctttatt ttttctgaat ataactactg tatcaaaaac aatcgattat
      181 gagacaaaac aaaatgacct cttttattta agaaaggagg tcattttttg tatcataaat
      241 tgtattaaaa tcaatgtatc actttttaat caattttaag accataaatt gcgtactaaa
      301 agaaattctg caattcaaaa taaagcattt taagccattt tcaaatttaa gtgcacccat
      361 ctacccgtca aatcacttta ttttccactc cagtagtttc ctaattcaac tgtgtgcaaa
      421 aacgatttct tctaaacaaa tcaagacaaa cttataaacc ttcatcccag caccataatt
      481 atttaaatca taggcatttt tcgaaattag tatatgtact caagtttcac tgcagattat
      541 ttatattttt tagagaaata aatttttcct attgacaaac acaaagaaag cggatacaat
      601 gcaattatgt cacatgacgt tatatgacgt tatgtaatat ctttacattt aatcagttac
      661 ttttattttg gataaaatta acatttttaa aattttaagg aggaaatgtt gatggatgta
      721 gcaaaaacgg ttaacgcaat tgtagaaaaa aatgatggag agatttctgg aatttatttg
      781 gtggcttgtg gcggttcgct ggtagatatg tatgtctcag attattttct taaagccgaa
      841 gcatcgaagt gtattacagg cttatataca gcgaacgaat ttaaccatgt aaccccaaag
      901 caattagatg ataaatctgt tgtcattcta tgttctcatg gaggaaatac agcagaaacg
      961 gtaaaagcag gagaagtcgc tcaagctaga ggagctgtga ctgttggtct gactcataat
     1021 aaaactgcgg atttattaaa ggtttctgat catagtttcc tttacgaatg gggagatgat
     1081 gcaaaaatag tcaataatcc tatggccatc attcttgatt taactgtatc tttggtcaat
     1141 gttaccgaaa catttgaaca ttacggtaaa ttcagagaag gcatgaaggt tatcgatacc
     1201 gtaattgaaa atggcattaa gcaggtgcag gatcgtatta aagtgtttgc tgataagtac
     1261 aaagatgaaa caatgtttta tatcttaggt agtggaccat catatggaca tgcttatggt
     1321 ttttcaattt gctctttgat ggaaatgcaa tggcttgatt caacttctgt tcattctgga
     1381 gaattcttcc atggaccttt tgaagtaaca gacaaagata cgaattttat tctcttgatg
     1441 agcattggac gcactcgtgc tttagatgaa cgggcaaaac gattccttga taaatatgca
     1501 gaaagagtag aaatcgttga tgcaaaggaa ctaggattgg atatgatatc agatgaagtt
     1561 tctgaattct tcaatcccat tttattctac agtattcttt cagaatatcg ttcagcttta
     1621 gcagatgcac gcaatcatga tttagatgtt cgtcgctata tggggaaagt ggagtactag
     1681 gaggattaaa atgaaggtat taggcttagg tgataacgtc gtcgataaat atgaaaatct
     1741 gggaattatg tatcctggag gaaattcatt gaattttgca gtttttgcaa aaaaactggg
     1801 acatgaaagt agctttatgg gagtttttgg ctcagatgag gaggcagctc atgttttatc
     1861 agcaataaaa gaaataggac tggacaacag tcactctcgc tttgaaaaag gcgagaatgg
     1921 ctgtgccaga gtgaagattg atgagggcga ccggattttc cttggcagca attcgggagg
     1981 tgttactcgt gaatacccga ttcagcttac tgaagaagac agagaatatt taaaccagtt
     2041 tgaattgatt catatgggat tatatagcca tgtcaatcat ctattagaag agttacaaaa
     2101 tgtttctgga aagatttctt atgatttttc tgacgacttt acggaagaag aggttcagcg
     2161 ggcaattgat aaagttgatt ttggcttctt ttctatagaa gacttttccg atgaagaagt
     2221 aaagcagttt ttgatgaagc atttttgtga gcgaaatgat gtattgattg caacccgagg
     2281 agaaaaaacg gctattggtt atgatggagc aaagttttat gaagtgaacc ccgtgctaga
     2341 agaaccagtt gatacaatgg cagcgggaga ttctttctta acagctttcc tactttattt
     2401 tctcgaaggg gacgatatag tcaccgcgat gaaaaagggg aatgaatttg ctgggaagtc
     2461 ttgtctggtt gacggttcgt tcggctttgg tattcattac gattgagctt gttttaaatt
     2521 cctgcatttg ataacctaaa gaaagaaatc ccggtaaagt ggaggttaaa aaaatgctgg
     2581 atacaaatgc acaattacca ttgtacttac aattaaaaga aatattaaaa aataaaatca
     2641 atacagggga gtatcccgag aatacgaaaa ttcccgctga atcagagctg atagaaagtt
     2701 atgaggtcag ccgaatcaca gtgagaaaag cagtagaaga actggtagaa gaagggtacc
     2761 tacttaaaaa acaagggaaa ggaacttttg tcaaccggca taaagttttt cgtaaaattg
     2821 aatatgttat gggtttcagt gagagttgta aatctaatgg ttttacaccg actagcattg
     2881 ttttggaacg caagatcatt ccagcaactg aagggatggc aaaaaaactc gatatcgctc
     2941 aaggtgatga tgttttatat atccagagaa agcgaatggc ggataacatc cccattatgt
     3001 tagaaaacaa ttattttctt atgaaagata tagtttctta atggaggaag agctgactga
     3061 ttctttgtat gaagtactta aagtccaagg taccttagcc atcaatcctg gggaaactgc
     3121 acttgaaatt gtcacagctg atgaccaaat tgccaaaata atggaagtag ctgttgggac
     3181 gccctttttc tttatggata ctttaatcaa cgatcaaaac aacataccaa ttcatgtagg
     3241 taatcaatat tacctgggag aagtatatcg tttttactta tgatttcctg ttttgatttc
     3301 aaggagtaga ttttatttat gaagcaaata ttgcggtcaa aaatgatttt tgatggagag
     3361 cagccacctt tcaaaggtta tattgtaatc aatgaaggga tcattgaaaa ggtggaaaac
     3421 aactggaatt acagtgatct attaaatgaa aacacggaat taattgctta tgatgatcaa
     3481 ttcgttatgc cagcaattca tgataatcat gtatttttca gtggttattt ggctatgaat
     3541 gctggcatag atttaagcga tacatcttca gctaacgagg ctgtagagcg tattaaagct
     3601 gtaatgaacc ataaaaaagc agatgaacca atttatgctc atggctggga tgctgccctg
     3661 tggaaaactg acccatcaga agagctattg aatgaaataa accactttgg tccaatcaca
     3721 gcgatcgaca aaaatcgcag ccattgttgg atgaatgcta ttgctaagca gacttatggt
     3781 tttacagaaa aagaaaccag tgctgaaagt cgagtattat taattaagga aatgctagca
     3841 aacaaaaagc ttttagcaga ggtttacgct gaatttgaag cattactgct ttcaaaaggt
     3901 gttatatcaa tgaaagaaat cgtttttgat gacgcagaat ttctttctaa tatacctcag
     3961 aggaaaatac ttagtaacct atatgtgcag gcagtccaag aacctctaca gcatgaactt
     4021 ctatatgagt ataaggaaaa agttttccct gaaaaggtcc gctttggtgg agtgaagatc
     4081 atggtagatg gtgttgttgc ggatagaagt ggagatattt acggtgttta tcagtcagga
     4141 gtagcttcac ccgagattga ctatgaggca attaaagcag aagttgctgc attcaatgaa
     4201 gtagagattc cttgctgttt gacaacggaa ggagataaag cgggttataa agctgcgtca
     4261 attcttgtcg aatttggaaa gcggttacct gaaggcgttt ttaattcgat tagtgatttg
     4321 gaaatggtta ctcaaaagac agcagaaaaa atgaacgaag gacaagtagt agcagaggta
     4381 tatccacaaa ttctcggctt gaatcaaacg ttagaagagg cttacatgtc aactgttata
     4441 aaaaat
//
LOCUS       DACCIQ010000136         4333 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCIQ010000136 DACCIQ010000000
VERSION     DACCIQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.141"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            64..297
                     /locus_tag="IVM62_002636"
     CDS             64..297
                     /locus_tag="IVM62_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846546.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(456..578)
                     /locus_tag="IVM62_002637"
     CDS             complement(456..578)
                     /locus_tag="IVM62_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6846547.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            768..992
                     /locus_tag="IVM62_002638"
     CDS             768..992
                     /locus_tag="IVM62_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846548.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1435..1641
                     /locus_tag="IVM62_002639"
     CDS             1435..1641
                     /locus_tag="IVM62_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6846549.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1641..1892
                     /locus_tag="IVM62_002640"
     CDS             1641..1892
                     /locus_tag="IVM62_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846550.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2073..2345
                     /locus_tag="IVM62_002641"
     CDS             2073..2345
                     /locus_tag="IVM62_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846551.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2790..2969
                     /locus_tag="IVM62_002642"
     CDS             2790..2969
                     /locus_tag="IVM62_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846552.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3028..3384
                     /locus_tag="IVM62_002643"
     CDS             3028..3384
                     /locus_tag="IVM62_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846553.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1526 a          675 c          836 g         1296 t
ORIGIN      
        1 tgatgatcta ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa aattagtctg
       61 acgatggatc tattgatacc cgtaattgca tttatagacg ggatttcttt gggcgtcact
      121 gaaaatcctt tggtaaatag aattgcgaaa ggatcactat ttcttgtatt cgtgtatctt
      181 gtctgttctt atcttttaaa aagagcagga aaggataatt ccttttggcg attgactaaa
      241 gtcacagtag cagcgacagc tgttatactt acggctattc taccgcttta tatgtagagt
      301 taacgggaaa tcgttgaact tcttgcgaaa tattgctata atgaacatga aaaagaggag
      361 ttgcgctaac aacccctcat taacagcacc gattaaagaa cggtggcaga atattagtta
      421 tttggaaata accgctgacc tgctaaagta gacggttatt tctttttgtc atttttaagc
      481 atatttacaa tcaaactaat caaagcaatg gtaaacatac caaaaccaag aatggtttga
      541 attgtctcat atgcggacaa aaggcttact cctttcatag attttagcac ttacattcat
      601 aagcaccacc acctttccag aagtcgccac cgtcttcaaa ctttactgtc ttgactatta
      661 tagcatatct aaaatcgaat aaaaattctc aattttagct gtttttgcta taattttagc
      721 gaggtgatcg tgatggaaca atatattggt ttactcgata aattagggtg atcaagacac
      781 agccattgct tgtgcgattt acattacgca cgagtcatga accaatcaat tgtgtagtgg
      841 ccaatataga aatagcggat aaattgctga ttttggaaga aggcaagtat actatttcag
      901 tgacaggtca cttcaataaa cgaaagcaac tagtcattga atcaatgcaa attagaaacc
      961 ctgatcattt tacgcgatcg atgagcatat aaaagaagag ctgcagtcct aaatgaggat
     1021 tgtagctctt tttgttttac atagaaacag taaaaatgaa aaggcctgtt taggaaggca
     1081 caagcgcctt ctcaattgaa agacctttct ctcactccaa ccgccaccgc cgttcgagaa
     1141 gaaaggaagt gaacgctacg cgttcactaa gataaagggt cttgcatagc aagacaaaat
     1201 agaaaaaatt tcctctcacc aaaaggttcg ctcctgttcg ttatctcttt attgatttgt
     1261 cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca
     1321 cgctgcgcgc ccttgtcttg tcgcttttcg ttgattcatt ctggccaaca aatcaataaa
     1381 gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc aatcatgcga
     1441 aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta tcatgcacca
     1501 catactgtcg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta ttgtttgtat
     1561 caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattatat tttaattgat
     1621 gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta aaagaagtcg
     1681 ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagtt ttacttattc
     1741 cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg ctatttgaag
     1801 acctagtgaa gattgcagga tatgaactgc attatcaaga aaacttggaa acttatgaaa
     1861 acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg gctcaaaatt
     1921 actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg gaggatatga
     1981 cgttcctata caacgaaaca cgtgttccaa aaaaacatta taaagaaaaa ttgtcggtag
     2041 ctgtggaaga aatgattgaa taaggtgtcg aaatgaatct tattgcgacg tactatcgaa
     2101 cactggaaga actgaaaaag caaaatgcaa aatggttttt ccaagcctta ctatgcttgg
     2161 aagtaggtgt caaaccaagc actattaaac cttctgaata ccaagcttta gaactaacgt
     2221 atgcgaaatt tatcgaaacg aaaaaagcca agactgtttc aagtgaatgg ttagattatt
     2281 ttgaaaatat caataagtat ggcgcatatt acaccatgaa aaaggaagac aacgaaaatg
     2341 aataaggtga tttatcaact aacaacggat tccatatcaa cgatttgaaa gaaaaaaagg
     2401 tcaataatca tttataccga aaagtgagaa tgtgtgaaac aaggacaaaa aaagcctagt
     2461 aactaaggaa atggttctta tttcgcacca atcgctccaa ccgcccccgc cgcttaggtg
     2521 gtgcgaaata aacgggcaaa gcccgataac ttaagagaaa gaaggtgatc catttgaaca
     2581 agattattcc cttattgtta gtactgtttg ttggcgaaat acttatgcga gatagtgcag
     2641 ctgtacagtt aaacctcgct gtatttttgg taatactggt gttttgtttg atcttttggc
     2701 ggtttgtatt agtcctcgtg agtctactat tcaagatgat tgcagccata tttttcatag
     2761 ggttatttcg atgaaaggag tgagtaccaa tgccaattgc aataggaaat aaacggttac
     2821 ccgtgacatt agatgaaaag agacaaaaag aattgcagca actaaagcag aagtatggca
     2881 aaagtgaatc taagattatg tgtattgcgt tagatctatt gatcgcgcaa gaaaaagcag
     2941 gatttgatgt accagcgcta aaaaagtgac gtcacttttt aatttgatct atataagtga
     3001 tatcactttt accaaggagg gaattaaatg ttaaaaacta aaaagaattc gtacaaatta
     3061 aagcttagta tactatctat tatttgtctt ttaggcatga taattagtgg tcatttgtat
     3121 agagtggcat atacgcagtt tattgagtac aaggttcact ctttaggatt agctggggat
     3181 cgatacgttg aatatgtcca ttattcatta aaatgttctt catatattct tctttggctt
     3241 attttttcat tgtttgaatt gctaaattta atagcaatat cttcatatca cttagataag
     3301 ctaaatacca aatctagccg ttataacgaa gtgtttaagg aaaatgtgaa gttagaaagt
     3361 gacttaaaca agctaaaaaa ataaaaagaa gaaaacataa gttaactaaa attattttat
     3421 taagaatgat ggaatataag gataaggtta ccaacataac gatcattact tctctgatat
     3481 gctatggact tctatcaatc agtattttgt ctatagatta tggagtaact atactgcaag
     3541 ccttagtttt ggctatgcct atgtatctat ctttgtttat catgcatgaa atatataagc
     3601 tatttgtttt atcaaaaaat gtagaaatac gggtaaatat tttggtgaat agttatcaaa
     3661 aagaactcaa agaaagaaga cggtggcggt atttattgaa aggatgaaaa agtaggattt
     3721 gacatactgg tattcagaaa gtgccgtcac ttttaaaaca atggaaaagg tggagataag
     3781 atgaagaaaa aacaaactaa actatcatta gcagatattt aacacagtta accgcaactg
     3841 aagatggtgt tgttgaattt gaacaatctg aaattgcggt agtagatgat cgttacctta
     3901 aaatgcctta cttctttgac caagcaaagg tcatttgcct ttgtcactac gatggggtac
     3961 gtgattttag gcgttaaaaa gtagtgatta tatcaacttt acgagtaaat ataaatttag
     4021 acatgaaact gaattatcca atggaaaatc atggtttata atctagaaaa attatagtaa
     4081 tgattggatt atatatattc tttcgtattt tcgtacaact gtagcttgtc aaaattgaga
     4141 aatttaaccg attaggtact ttacatcgtt atttgggtaa tttctaaaac atgaagctta
     4201 ccaacgaata aactgagact atcttattaa ttgatcaata taagaaaata tctttgagga
     4261 gatgatgtac tatgttttcg tctatccacg attggttagg tggtcttttc ggcggtggag
     4321 gtaaaggttt tta
//
LOCUS       DACCIQ010000137         4299 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCIQ010000137 DACCIQ010000000
VERSION     DACCIQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.142"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1351
                     /locus_tag="IVM62_002644"
     CDS             <1..1351
                     /locus_tag="IVM62_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAP6846554.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYH
                     VEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSL
                     GYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVL
                     EQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIP
                     ARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1461..1511
                     /locus_tag="IVM62_002645"
                     /pseudo
     CDS             1461..1511
                     /locus_tag="IVM62_002645"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            1545..2921
                     /gene="tet(L)"
                     /locus_tag="IVM62_002646"
     CDS             1545..2921
                     /gene="tet(L)"
                     /locus_tag="IVM62_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6846555.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            3485..>4299
                     /locus_tag="IVM62_002647"
     CDS             3485..>4299
                     /locus_tag="IVM62_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6846556.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYK"
BASE COUNT         1408 a          655 c          883 g         1353 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa
       61 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
      121 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
      181 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
      241 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
      301 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
      361 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
      421 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
      481 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
      541 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
      601 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
      661 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
      721 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
      781 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
      841 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
      901 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt
      961 tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa
     1021 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
     1081 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
     1141 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
     1201 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
     1261 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
     1321 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
     1381 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
     1441 aattaattat gaagtgaaat gaatgtaaca gggttcaatt aaaagaggga agcgtatcat
     1501 taaccctata aactacgtct gccctcatta ttggagggtg aaatgtgaat acatcctatt
     1561 cacaatcgaa tttacgacac aaccaaattt taatttggct ttgcatttta tcttttttta
     1621 gcgtattaaa tgaaatggtt ttgaacgtct cattacctga tattgcaaat gattttaata
     1681 aaccacctgc gagtacaaac tgggtgaaca cagcctttat gttaaccttt tccattggaa
     1741 cagctgtata tggaaagcta tctgatcaat taggcatcaa aaggttactc ctatttggaa
     1801 ttataataaa ttgtttcggg tcggtaattg ggtttgttgg ccattctttc ttttccttac
     1861 ttattatggc tcgttttatt caaggggctg gtgcagctgc atttccagca ctcgtaatgg
     1921 ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa agcatttggt cttattggat
     1981 cgatagtagc catgggagaa ggagtcggtc cagcgattgg tggaatgata gcccattata
     2041 ttcattggtc ctatcttcta ctcattccta tgataacaat tatcactgtt ccgtttctta
     2101 tgaaattatt aaagaaagaa gtaaggataa aaggtcattt tgatatcaaa ggaattatac
     2161 taatgtctgt aggcattgta ttttttatgt tgtttacaac atcatatagc atttcttttc
     2221 ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca tatcaggaaa gtaacagatc
     2281 cttttgttga tcccggatta gggaaaaata taccttttat gattggagtt ctttgtgggg
     2341 gaattatatt tggaacagta gcagggtttg tctctatggt tccttatatg atgaaagatg
     2401 ttcaccagct aagtactgcc gaaatcggaa gtgtaattat tttccctgga acaatgagtg
     2461 tcattatttt cggctacatt ggtgggatac ttgttgatag aagaggtcct ttatacgtgt
     2521 taaacatcgg agttacattt ctttctgtta gctttttaac tgcttccttt cttttagaaa
     2581 caacatcatg gttcatgaca attataatcg tatttgtttt aggtgggctt tcgttcacca
     2641 aaacagttat atcaacaatt gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa
     2701 tgagtttgct taactttacc agctttttat cagagggaac aggtattgca attgtaggtg
     2761 gtttattatc cataccctta cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa
     2821 cttatctgta tagtaatttg ttattacttt tttcaggaat cattgtcatt agttggctgg
     2881 ttaccttgaa tgtatataaa cattctcaaa gggatttcta aatcgttaag ggatcaactt
     2941 tgggagagag ttcaaaattg atcctttttt tataacagga attcaaatct ttttgttcca
     3001 ttaaagggcg cgattgctga ataaaagata cgagagacct ctcttgtatc ttttttattt
     3061 tgagtggttt tgtccgttac actagaaaac cgaaagacaa taaaaatttt attcttgctg
     3121 agtctggctt tcggtaagct agacaaaacg gacaaaataa aaattggcaa gggtttaaag
     3181 gtggagattt tttgagtgat cttctcaaaa aatactacct gtcccttgct gatttttaaa
     3241 cgagcacgag agcaaaaccc ccctttgctg aggtggcaga gggcaggttt ttttgtttct
     3301 tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg gttttggtcg gcactgccga
     3361 cagcctcgca gagcacacac tttatgaata taaagtatag tgtgttatac tttacttgga
     3421 agtggttgcc ggaaagagcg aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt
     3481 acatatgagt tatgcagttt gtagaatgca aaaagtgaaa tcagctggac taaaaggcat
     3541 gcaatttcat aatcaaagag agcgaaaaag tagaacgaat gatgatattg accatgagcg
     3601 aacacgtgaa aattatgatt tgaaaaatga taaaaatatt gattacaacg aacgtgtcaa
     3661 agaaattatt gaatcacaaa aaacaggtac aagaaaaacg aggaaagatg ctgttcttgt
     3721 aaatgagttg ctagtaacat ctgaccgaga tttttttgag caactggatc caggagaaca
     3781 aaaacgattt tttgaggaaa gttataaatt attttccgaa cgatatggca agcaaaatat
     3841 tgcttatgca acagttcata atgatgagca aacccctcac atgcatttag gtgttgtgcc
     3901 tatgcgtgat ggaaaactgc aaggaaaaaa tgtgtttaat cgtcaagaac tgttatggct
     3961 acaagataaa ttccccgagc atatgaaaaa acagggtttt gagttgaagc gtggtgaacg
     4021 tggctctgac cgtaaacata ttgagacagc taaatttaaa aaacaaactt tggaaaaaga
     4081 gattgatttt ctagaaaaaa atttagcagt taaaaaagat gaatggactg cttatagcga
     4141 taaagttaaa tcagatttag aagtaccagc gaaacgacac atgaaaagtg ttgaagtgcc
     4201 aacgggtgaa aagtccatgt ttggtttggg aaaagaaata atgaaaacag aaaagaaacc
     4261 aaccaaaaat gttgttatat cggagcgtga ttataaaaa
//
LOCUS       DACCIQ010000138         4297 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCIQ010000138 DACCIQ010000000
VERSION     DACCIQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.143"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            109..1068
                     /locus_tag="IVM62_002648"
     CDS             109..1068
                     /locus_tag="IVM62_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6846557.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1185..1493
                     /locus_tag="IVM62_002649"
     CDS             1185..1493
                     /locus_tag="IVM62_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6846558.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            1520..1819
                     /locus_tag="IVM62_002650"
     CDS             1520..1819
                     /locus_tag="IVM62_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP6846559.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            1993..2544
                     /locus_tag="IVM62_002651"
     CDS             1993..2544
                     /locus_tag="IVM62_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP6846560.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            2690..2983
                     /locus_tag="IVM62_002652"
     CDS             2690..2983
                     /locus_tag="IVM62_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846561.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            2976..3272
                     /locus_tag="IVM62_002653"
     CDS             2976..3272
                     /locus_tag="IVM62_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846562.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            3343..>4297
                     /locus_tag="IVM62_002654"
     CDS             3343..>4297
                     /locus_tag="IVM62_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6846563.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDG"
BASE COUNT         1587 a          658 c          843 g         1209 t
ORIGIN      
        1 acaatttatt aaaacgtcga ttgtaaaatt aagctagaca actaaaaaga tcatttgtgc
       61 tacactcaaa atagttccga ccaaagaatt aataaggagt gagtacaaat gacttacacc
      121 catcttacaa cagacgaact agtaatcata gagtcttatt tcaatatcga gaaacctgtc
      181 gctcttgtcg cacaaagttt aaatcgctct agacaaacca tttataatgt ttattcgttt
      241 ctcaaacaag gaaaaaaagt gctagattat taccgtcaat acaaacaaaa taaatctcgt
      301 tgtggacgac gttcaaccgt tttatctgat gaacaatcaa catatgttca agaaaaggta
      361 gctcaaggat ggtcacccga tgtgattatc ggccgtggcg agtttccaat tgattgttct
      421 gttcgtacac tctatcggat gtttaaaaaa ggcctttttg acacacagtc gttacctatg
      481 aaggggaaac gtaaaccgaa taatcatcaa gaaaagcgtg gaaaacaggc ctttcgcaga
      541 acgattcatg aacggaacag tctctatcca cacctgacta aagaatttgg tcatctcgaa
      601 gtcgacacca ttgtgggagt taaacacaag agcgctgtta ttacattggt tgagtgttta
      661 tccaaggtta ttattacttt gaaaccgaca ggcagacgag ccgaagatat cgaaaacagg
      721 ttaaatgatt ggttcaaatc catgcctaaa cacttgttta aatccatgac atttgattgt
      781 ggaaaagaat tttccaactg gcgatctata agtaactcga acgatattgc catctttttc
      841 gcagatccag gaacaccttc gcaaagaggg ctaaatgagc actcaaacgg cttgcttaga
      901 cgagatggct tacctaaaac aatggacttt agggacacgg atgaaacctt tattcaatcc
      961 gttgcctcga aaagaaacca tattccaaga aaatcattaa attatcgaac accattggaa
     1021 gtatttttga gctgcataga taaagacata ttgtctagct taatttgaca aatcagatag
     1081 tataaaatga atcaaattga atatcaaata tatattatat ccatgaagcc caatcttctt
     1141 taatagaaga ttgggctatt tttatggcaa aggagggaaa agaaatgttt caaaataaag
     1201 gacaacgtta tgtcacaaag ggagtaatgg acagtttgcc ggtggaatta caagctttat
     1261 gttggaatct aatcgatcaa aacgttcaaa agcaatttca tttagactat cttcaaatct
     1321 ttgaattttc tacggaaaaa ggaaatcaaa agttggttca tcgtcaagaa gaaccagaag
     1381 caagaaaaga gtatctgatt tcacctaagc ttcgtttgaa atcagtgtct caaaaaatat
     1441 gggtcattga ttcgggtgag tatcaaacga tgttattacc agaagaatac tgatatctaa
     1501 aagaaaggaa cgaaaggact tgttagaaac ggctatcgaa gtactggaaa agtgtgcaca
     1561 gctagtcact actcctgaag agtggggcta tgaatccgtt acgatggaaa aagaggaaat
     1621 tgaaatgggt atgcttcctg aagatgttac tctaccacgt ttagtgatga cacatcttta
     1681 tatttattgc gcaccagaag atggaaaaga ctatgtagtt tatttcatta cagatattac
     1741 cagccagcga gaatttgttc gtgggttact ggtagaagga cgattggttt ggagtcagat
     1801 tggaggaacg aatgaataag atcgattatt tgagaatgat tgcgaaatgt ttagaagata
     1861 tccttacagc gattcatcaa attctgaaga aaaaaggaga aagaatatga aatattttca
     1921 actagtattg acacttataa cggcaatggt gattgaattg cttgaacaaa aattagcata
     1981 ggagaaaaag agatgattga aaaagaatta gtgaccacac aaacagaagt ttggcgagat
     2041 cctcatcata cccatcgcta tgttttcaaa cgacaatggg cgatcaaagg aaaagaagcg
     2101 gaagataaat tagcggtagt cattactatt cgtccaacag atacagaacc atttacgaat
     2161 gatttgagta tgttattaat cgaaaagaat atccgacaat taggatttac tggttttatt
     2221 gctgtgaatc ttttttcgta tacaaaagcg aaaagtcctc gggtatttcc tcgtggaaat
     2281 gatgaacaat ctttggaaat attaacaact gttttcacag aaaagaaaat caacaccatt
     2341 atttttgctt gtggttcgat tacagcgacg agtcaagtgg cttatgaaca agcgaagacc
     2401 atttacgatc aattgagtgc gaaacaaaaa aagatgacca aagtattgat aaatgcagaa
     2461 ggtaagttat cccacccact aagtattcat gttcgtaatg aatggcaatt ggcagatagt
     2521 caattattat ttcaagaaaa ataacatttt attttcttat gttacttata agtagtttta
     2581 catgcttttt gactttaaaa atacatgtga tgatacatgt tatatgaatt atcaatgtac
     2641 ccagtaatat gctattttca ttatgtaaaa aataacaggg ggtgtgtaga tggagattga
     2701 tgaaacttgt ttgagtaact cttataaaag tatgaatgac ttttttgagg aaatggaact
     2761 ggattcagaa ttgctctatc aattttatct tgcttttcga gggcaacaga tttcttttcc
     2821 aatgaaaatg tacgatcgtg agttagttag aaaaagaatc gaaaacatga tagaacagga
     2881 aaaaactgta gatatccgtc aattgacaga agtttatggt ttctctgcaa gatggattca
     2941 aaaagtaatt aaacaaaaag aaaggagacg agtacatggc taagctgatt gtattaggtg
     3001 gtttaattta cggtggatat tggttatata aatatatgct tgcgcatccg aatatagata
     3061 aaggaaaagt aattaagaca gttggggtaa tcatagttgg actcatagct ctaactaaga
     3121 aaaataatac taatagccca aaaataatga gaacttatcg ctgtagagga tgtggaaggg
     3181 aaatttctac tagttttaat ccatctgaaa gccctcaagg aagagatcgt tgtcatgctg
     3241 gctatcgaca ttcttgggaa aggattaact aaatgaacac cattgagatt aattgactga
     3301 taaaataagt acgcgaggga gaaattgaaa gggggaaata aaatgaaaaa atggttgtta
     3361 ttactaattg gaatggggat tttattcact ggttgtacat ctgagaatac gaggactaaa
     3421 gagactgtag aaactgatac aacaattgag gctaaaaaaa gtaaaacgca atcaactgaa
     3481 caaactacaa cagaaatgaa acaatctgtg gaagcaagta caagtgaaag ttcaatcatt
     3541 gaatcttctt ctgaagaggt taaaaatact ttgtgggata gtgagaaatc tactcaacta
     3601 gcaatttttg tcccacaatg gggaaagaca ttaggtcaag aatataaaag ctacaatcct
     3661 cagaacaatg taagtctgta tggtacgcct ttaccaagtg cagttattga tggaaattgg
     3721 aaaatggcaa tcaatgaagc accagttacc gtacaatggt cagaagatgg aactggtcat
     3781 gcagatttca atttagtagc agtttattct gatgtagaaa ctggagaata tttagggcag
     3841 catgtatatt tctttggctt tcaaaatgga caacctaaag tttatttaac gcaacaaaat
     3901 caaggcaatg ataaaaacta catgtacttc aatgaaactc agaaccaaca gttaaaacaa
     3961 ggtttcattg atattgtgaa tggacaaact cctcaaactc cagtagtaga agaatctact
     4021 caacaagcaa caactacaac gacacaatct acaagtagct cagcaatttc aagttgggaa
     4081 gctgctaaag aaactgtact aaataataaa gctttatggt tgtatgattc tcctgataaa
     4141 aatgcatcaa atactaattt aattagcaca aatgaatata tgaaagatct aatgcaagat
     4201 gaaaatggaa gctactattc aattaatatt gtaacggatg ataatggaaa tacaacaatg
     4261 gctggtggaa aacaattccg tgtttataca gatggaa
//
LOCUS       DACCIQ010000139         4248 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCIQ010000139 DACCIQ010000000
VERSION     DACCIQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.144"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            563..1060
                     /gene="dfrG"
                     /locus_tag="IVM62_002655"
     CDS             563..1060
                     /gene="dfrG"
                     /locus_tag="IVM62_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP6846564.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1132..3084)
                     /locus_tag="IVM62_002656"
     CDS             complement(1132..3084)
                     /locus_tag="IVM62_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP6846565.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3091..3387)
                     /locus_tag="IVM62_002657"
     CDS             complement(3091..3387)
                     /locus_tag="IVM62_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846566.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3565..4248
                     /locus_tag="IVM62_002658"
                     /pseudo
     CDS             3565..4248
                     /locus_tag="IVM62_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1303 a          725 c          698 g         1522 t
ORIGIN      
        1 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
       61 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
      121 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
      181 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
      241 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttta agtagttcag
      301 ttttggagta caaaatgaca aaatgtactt caaaatggac tacttttttc attttaactt
      361 acataccaaa ttacatacaa aatgatttat aaaatgcaat tgaaaatgat aattgaatga
      421 aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac
      481 taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt
      541 agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag
      601 agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa
      661 aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag
      721 agccttacct gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg
      781 ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat
      841 ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta
      901 catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga
      961 ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta
     1021 ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa
     1081 gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa
     1141 ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt
     1201 caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat
     1261 tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc
     1321 accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat
     1381 aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc
     1441 cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa
     1501 tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc
     1561 ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg
     1621 tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg
     1681 tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg
     1741 atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga
     1801 gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca
     1861 atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct
     1921 attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga
     1981 tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag
     2041 atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc
     2101 ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata caactatata
     2161 cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg
     2221 gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt
     2281 tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca
     2341 ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac
     2401 tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg
     2461 ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca
     2521 ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt
     2581 gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac
     2641 atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga
     2701 taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta
     2761 cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc
     2821 ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca
     2881 gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa
     2941 cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct
     3001 atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat cttccttcgt
     3061 ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga tacccatttt
     3121 ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc
     3181 taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg catattcagc
     3241 gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag acttagcgtt
     3301 cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct tagtaaattg
     3361 taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt cgcattttta
     3421 tatacatttt aacttacatt ttgtggtgta aattaacaac aaacgttgat ttaacaataa
     3481 aaaacgagta tcaaattcag ttgcaatttg atactcattt aggtttacat tttcactttt
     3541 tgtactccaa aactgaacta cttattttta tttagttgtc tctatatttt ctctgcttat
     3601 cggacagtag acatctacta tcgttccgca attgccgaag ctatcgcaat aacacttgtt
     3661 atccctgtat tattttatgc ttatcaaatc atctctggaa aagaagaaaa gtatccgtct
     3721 gtaaagctag cattgagtat gagtttgcta gtgtacagcc atgtactatc aacattaatg
     3781 agtacagcat taattatcat ttttattttt attcgtttgg tttctaaagg tttcaagaat
     3841 gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac attggttctg
     3901 acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat caacaaacct
     3961 agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac tgaagctatg
     4021 aacaacgact taactactta ctcaatggga ctagttggtc tgttatcttt atgtattccc
     4081 ctgattttat ttaaaaaatt aacaaacata gaaaaaaaga tttattatgg tacgtgtttg
     4141 acttggctag ctacaactag tttggtgcca tggtatcttc tgcaaaatac gccagccaaa
     4201 ttgttacaat ttccttggcg gatcttaagc ttgcaaataa tattctct
//
LOCUS       DACCIQ010000140         4221 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCIQ010000140 DACCIQ010000000
VERSION     DACCIQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.145"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            89..790
                     /locus_tag="IVM62_002659"
     CDS             89..790
                     /locus_tag="IVM62_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6846567.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1362..2378)
                     /gene="tsaD"
                     /locus_tag="IVM62_002660"
     CDS             complement(1362..2378)
                     /gene="tsaD"
                     /locus_tag="IVM62_002660"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP6846568.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2375..2839)
                     /gene="rimI"
                     /locus_tag="IVM62_002661"
     CDS             complement(2375..2839)
                     /gene="rimI"
                     /locus_tag="IVM62_002661"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6846569.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2846..3388)
                     /gene="rimI"
                     /locus_tag="IVM62_002662"
     CDS             complement(2846..3388)
                     /gene="rimI"
                     /locus_tag="IVM62_002662"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6846570.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3372..4196)
                     /gene="tsaB"
                     /locus_tag="IVM62_002663"
     CDS             complement(3372..4196)
                     /gene="tsaB"
                     /locus_tag="IVM62_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP6846571.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1248 a          893 c          733 g         1347 t
ORIGIN      
        1 tgtttctaat gttggtatgt taaaacttta caaattcttt aaaaaaagtg acaataaata
       61 tagtgaacaa taaggagagg actttttaat gcgtgttaat aaaatcgatt ttcacttttc
      121 aaaactcaga tatcaacctg attttgaaca tttctcggat gaagagttcc aacagatcaa
      181 ggaacatact gttcttcgtt cttataaaaa aggacagatc cttttcgacg atggtgatcc
      241 cagacaacgg ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag
      301 tgcaacttat tactactatg actatgtgaa tacaaataac ctttttccta tgtcaggaat
      361 gtttggagaa gagacctaca cttatacagc tcaagcgatg actgatattg aaacattcta
      421 ccttcctgtt tcctactatg aaaaattagc acgaaaaaat cccgaacagt tgatctatat
      481 gatacaaaaa atttctaaag tagttgaaat acatgaattg cgtatccaaa tcggattgac
      541 ctctagcgcc tttgaccggg taaaacaaaa tttattcatt ttgaaagaag aattgggcgt
      601 accaacaaaa gatggtcata tcaccattcc ttatccgatc accttaaaag aattagcagt
      661 gaatagcggc acgacacgtg aaactgctgg acaagtaatc aaacaactac gtgattccgg
      721 actgttagat tatgacaaaa agcgttttac tttctgtaag acagctacta ctccagctat
      781 gagagaataa cgcgaacaaa aataagcagt gaccctgata tttatcaggg tcactgctta
      841 ttttcctccc taaaacttct gaaagatccg gcagttttag atcactgggc aagactcaaa
      901 gattttctat gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat actgatagat
      961 taactagagg aagcgtcttg tcctcatccg acctagatta ttcgatattc ctcgacaata
     1021 aacgattaat cagtgagggc aatcagagga acggataatg atgaataagc tgtaaaaaat
     1081 agggctttga caaaagcgtg tcacagccct tatttcgatt aaacggtgtg tacctgtcaa
     1141 ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg aaattgttcc
     1201 ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg ttcaaaagct
     1261 tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt ttcctgggct
     1321 gttcttatta cttgatgcag ggcagatttt tcacaacctc tttattcaga taaaaccaag
     1381 cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc cacatgtgct
     1441 gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat cactagttct
     1501 acctcaggga gtttagttgc caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc
     1561 gctacacctc cagctacaat cagttgcttg actggataat tttgacaagc tcgaagtgtt
     1621 ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc atttttgtct
     1681 agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact ttttaatccg
     1741 ctaaaactga aatcataatt atcttcatgg atcatcgctc gtggaaaatg gtaattatct
     1801 tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag acctaatact
     1861 cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc aatgatttca
     1921 tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac aagaagtgcc
     1981 atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc tgccatatgg
     2041 tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc agaaatacca
     2101 atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga taaatcttct
     2161 gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg ttctacgtga
     2221 tggcggctag ctacttcagg aactacgcca ccaaaacgtt tatgactgtt tatttgagaa
     2281 gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact cgtttcatca
     2341 caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc gtttttaact
     2401 ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc cgtttaccta
     2461 atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat agacgtactt
     2521 ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt aacaaatgcc
     2581 gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc tcgacttcat
     2641 ctattacttt cgagtatcca ataaagccac agatctggtt atcttcaact aaaagcagat
     2701 aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat
     2761 gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct tcatcaaaat
     2821 ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc atatccaacg
     2881 catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga aaacctaact
     2941 tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac atcgtctcac
     3001 agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg attcgtttac
     3061 gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg aacgctcttg
     3121 aaccgataaa accaatcatt cttccattat caaagatacc tagatataga tgaggaagcg
     3181 gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca ttatagactt
     3241 ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt agtacgtaac
     3301 gacgatcgat ttgttcgatt gttttttctg tataaatcat tttatcttga aaaaagcttc
     3361 tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac
     3421 catttttctt ctgcttctac ccgttttaaa tagcgaggaa gaaaagcttg tttctctgca
     3481 actggttcag ctaaagcacc taattgagcc acagttacac cacttggaat atcccatgct
     3541 ggaactgtat taatcatcgc actaggcaat atttccttta tttcatagaa gaatttgtga
     3601 caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt gaacaaatca
     3661 gaagcagaaa tatgtacatc ttcaagtaca atttctaatc tattttcttt ccagcggtat
     3721 gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat tttatccaca
     3781 ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc tttatctaat
     3841 gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata agaaccggga
     3901 ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc tgcaaacaat
     3961 tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt ttgtatttgt
     4021 ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc agtatctatt
     4081 cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc cattgtagca
     4141 tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaatg atacatgatt
     4201 atctgctggc gggcaaattt t
//
LOCUS       DACCIQ010000141         4066 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCIQ010000141 DACCIQ010000000
VERSION     DACCIQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.146"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            239..1189
                     /locus_tag="IVM62_002664"
     CDS             239..1189
                     /locus_tag="IVM62_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6846572.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(1234..1867)
                     /locus_tag="IVM62_002665"
                     /pseudo
     CDS             complement(1234..1867)
                     /locus_tag="IVM62_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(2127..2675)
                     /locus_tag="IVM62_002666"
     CDS             complement(2127..2675)
                     /locus_tag="IVM62_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846573.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2676..3533)
                     /locus_tag="IVM62_002667"
     CDS             complement(2676..3533)
                     /locus_tag="IVM62_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6846574.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3819..>4066)
                     /locus_tag="IVM62_002668"
     CDS             complement(3819..>4066)
                     /locus_tag="IVM62_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6846575.1"
                     /translation="LKKLKEKPLKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFK
                     YAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT         1237 a          773 c          605 g         1451 t
ORIGIN      
        1 taagacctag tataaaaaga atgggttcat tttttctttc ttgcaggatg aggtttttta
       61 tataaacttt atttgatata aagaaaaggc attcataatt gtgctatact actaaaaaat
      121 gataatacag tgaattagat tagcgcttta ttaaattaag aggacagcat atcgatattg
      181 cttccttttt tttgctattt atttaaggga tagaagttta gaaaggactg tttataagat
      241 gaaaaatgat atagtgactt atgcaaacac aaaatgtgaa gaaattggca tcagtaattt
      301 agaagagtgc ttttttgaca caaatgacct gtcgcaattt ttaagaacgg gaagaacaaa
      361 tgccagtaaa cttgccaatg acttagttgc aaagaacgtt ctatttaaaa tcaatactcg
      421 tccagtgtta tttgccatca aagagcgctt tgaagagcac tttttgattc cggtaaaaga
      481 tgtatacgaa agtatatctg aattaaaaac agacttggag cccaattcta cagattccat
      541 tctacaaaag atgattgggt atgatatgag cttatccgat gcggtggaac aaatgaaaac
      601 tgctgtcttc tatcctggaa gagggcttcc tatgatgatc actggagata ctggagttgg
      661 aaaaagtttt tttgttcata ctgcttatca gtttatgaaa aaagtgaacg ttattcatga
      721 gaatgctcct tttaaagtac ttaattgtgc gcaataccat aataatcctg aattattgtc
      781 ggggatacta tttggctatg ttaaaggtgc ttttaccggc gcagatgaag atcgaaatgg
      841 attgttgcaa gatgcagacg gtggagtact gtttttagac gaagtacaca gattgcctcc
      901 tgaagggcaa gagaaactat ttgtttatat ggatggcgga acttattcta gagtcggtga
      961 aactatccat cacgcctcca atgtacgttt agtttttgcg actacagaag gcaaagagga
     1021 ttttctggaa acctttatgc gaagaatccc tatacatatc catatcccaa atattgagga
     1081 acgaggtatt ttagaaaaaa aacaaattat cgaaaatact ctctttagcg aaagcaaaaa
     1141 ctttagcaag gctattgatg tttctcctaa agtcactagt gttgcataaa tatagtcaga
     1201 atattctgaa ttattatttt cttgaaaaaa gtaaaaaaaa tcgtatccca aactttcatc
     1261 tacatttcca gaaagatctt catgttgata atcaattcct gaatcaatga ccgctacctt
     1321 cacatcctta gaacccttgg tttcttccca agcggatggc atgctaatct ctcttaaatt
     1381 agcttgttta ctccaattag gatcgttatc gatttcctaa agaatcagtt gtatttggct
     1441 cctcaacatc attcaacggc tctagttcaa ccacatagtt tggctctgca taattgacca
     1501 tatcagaagc attaaatgtt cttactgcct cttccacact aatttcttcg ttcaatttca
     1561 ctaataatac ttgatctcgc ttattataat cgctttcatt tgtcctaaga gtcaaatctt
     1621 tttccaccat aacttttaca cccaataaat tttgcggttt atttttctct gttgaaattc
     1681 tttgtgtagc ctttaaactg acaattagct cattgggtac atactctatt tgagagtcat
     1741 ctaattggct cataatcctt tctttttggt tagtatctgc agttacttgt ttagtatgag
     1801 ccatgaaccc aaacatactc accccaacaa ccaatcctaa atatattttc tttttaaact
     1861 ttttcatcta catctcctcc taatagtttt attttagagt tagctctatt acttatttac
     1921 taactcaaca acagattatc atacacttgt taatgaaatc acaatatctc attaaaacac
     1981 accctaaaat cataaccgtt cctatgctca ttttttggat tttttaatca cgtaaccaga
     2041 aaaataaatc ccctctattt cacgcaaatc aaagaagaac taagtaacta ccgtttaaag
     2101 gtagttactt agttcttctt tgattattat ctaactttat tattcattcc ccaccctttg
     2161 cgcagtgagt cttcggtatc ttgttttatc tgactggtag ttcttattat ttctccacga
     2221 tcatctaatt ctggaacaat tggatatttt tctctgaaca agtctagttc tttcaccacg
     2281 tcattaaaca aatcttgttt atcttcttgc gtaagattct gtttaatata ctcatctaaa
     2341 gcttttctaa gcaattcatc tcttgtcata ttagtctcat acttttctac taaaactctg
     2401 gaagtcgcat ttaacaaagt cgacagtttt aaaggatgct tggtggaata aaccttatca
     2461 aactttctga ttggtgcgcc aacttttttt gttcttcctc tactctgtct ttcaacattg
     2521 gaggaagttt ttatttcttg aacttggtgc acttctaatt gcttattttc agatggtgta
     2581 attgcttgct gctttttctt tgcttctgct tcttttttta ttttggcgag tcctttgttg
     2641 ttcccaaaaa tatcaagtcc tttgatgtca gccaattact ccacctccgc ataccaattc
     2701 tcgtgttctt ccaattcttt caaaatactg ataaataatt cgtggctttt gcgatcccat
     2761 tgttttaaga gacctttctt tgtcttctcc atcgtaatac ctgatccgtc gtatcgtttt
     2821 aagcgcgcat ttttgagtac aacattttta atcaaatgtt ccccataaat ctcttgggct
     2881 tgtttataca tttcctgatc tactgcgtca ccgctttcca acatgaaagg aataacacca
     2941 gcaacatata aatttgtatc aaattctttc acaaaaaagt ccatatactc aagatattgc
     3001 atcgcatttc tcatcgattt tacttgggtc tgtaagacga cgattgccca atccgcaatg
     3061 tacatcgcgg atttactata tacagaaata gaaggtggga catctaaata gatgacgtca
     3121 aactcatcaa ataatggttc tagcttttct ttcatatatt tgaattggga taatgaatca
     3181 ttatattggt ctttgatcca atcatcatac atttcaaagg acatgtctgc tggaagtata
     3241 tagaggttat tgattacttt atagacagcc tctttaggtg gaagattgtt tatcaacacg
     3301 tcagtcacag tattttcgta aacaatcggt tcaccttctc cgcctccttc ttcgttagtg
     3361 aagttcatat gcgttaagtt catcacatca gaagtatttc cttgcatatc caagtcaatt
     3421 atcaaggttc tttttccttc attaactgct gattcaaacc ctaacatttg cgcgcacgta
     3481 cttttaccta cgccaccttt aaaattaccg attacatatt tcttcgctac cacgttccta
     3541 cctcttttct tttagcaata ttcatttgat actattataa catatttatt tttagttact
     3601 accaaaaaaa agatatatat gtactacctt ataattaata agtaaaaggt agtacataaa
     3661 taaggaaaag ctgaaatata ttgatttata gctttcctca aagtaatgac gttatttaat
     3721 ttatcgagaa gcagagaata ctcgcgactt tagtcatgta gacgaatcag attttcgata
     3781 aaaaagaagc cgtccttctt aagaacgaca acaaatcact agattaattt tttcaactgt
     3841 tcataaaagt tttcatgcgt tccacataac aatactggaa taaccttgtt ttctataatc
     3901 tcgtaagcta ctctatatgt tgtgcccgca tatttgaacc cactagacca aatacctgat
     3961 aaatccccag ttttttgttc acccttatat ggatcaatcg caatttcatc ataaattaaa
     4021 ttcaaaaatt tttctttcaa tggcttctct tttagctttt ttaaat
//
LOCUS       DACCIQ010000142         4027 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCIQ010000142 DACCIQ010000000
VERSION     DACCIQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.147"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(162..1418)
                     /locus_tag="IVM62_002669"
     CDS             complement(162..1418)
                     /locus_tag="IVM62_002669"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP6846576.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            1998..3299
                     /locus_tag="IVM62_002670"
     CDS             1998..3299
                     /locus_tag="IVM62_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6846577.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3368..3967)
                     /locus_tag="IVM62_002671"
     CDS             complement(3368..3967)
                     /locus_tag="IVM62_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6846578.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         1211 a          801 c          692 g         1323 t
ORIGIN      
        1 tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa
       61 agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg
      121 taatagtatt tccctttttt aaggtaaaaa cttactaaat tctaatcaat tactttggcc
      181 aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat
      241 ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt
      301 gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc
      361 acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg
      421 atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc
      481 gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt
      541 tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct
      601 tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac
      661 cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct
      721 gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac
      781 gcttctgctt ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc
      841 cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag
      901 tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg
      961 ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc
     1021 aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt
     1081 gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact
     1141 tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct
     1201 gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc
     1261 ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa
     1321 agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta
     1381 atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt
     1441 ttttatgaaa gcaggaacct gctagcatat gtaattaatt atttcttcag gataggatag
     1501 gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa
     1561 tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca
     1621 ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac
     1681 cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat
     1741 taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt
     1801 ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat
     1861 tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt
     1921 actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa
     1981 agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt
     2041 taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag
     2101 aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc
     2161 aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg
     2221 cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac
     2281 taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct
     2341 ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat
     2401 acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa
     2461 atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt
     2521 cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg
     2581 tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata
     2641 acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca
     2701 tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa
     2761 aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc
     2821 aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac
     2881 agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc
     2941 aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta
     3001 tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc
     3061 cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct
     3121 gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg
     3181 ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc
     3241 ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat
     3301 tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt
     3361 ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg
     3421 tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa
     3481 aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc
     3541 gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag
     3601 aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt
     3661 tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg
     3721 gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa
     3781 atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta
     3841 cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca
     3901 tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac
     3961 cgttcatttg tagagcctcc cgtaatttta aacttttata atatgtatag ttcaaattta
     4021 ttctgag
//
LOCUS       DACCIQ010000143         4025 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCIQ010000143 DACCIQ010000000
VERSION     DACCIQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.148"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            842..1054
                     /locus_tag="IVM62_002672"
     CDS             842..1054
                     /locus_tag="IVM62_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846579.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1332..3131)
                     /locus_tag="IVM62_002673"
     CDS             complement(1332..3131)
                     /locus_tag="IVM62_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6846580.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3255..3851)
                     /locus_tag="IVM62_002674"
     CDS             complement(3255..3851)
                     /locus_tag="IVM62_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6846581.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1444 a          712 c          688 g         1181 t
ORIGIN      
        1 tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc ataaaaaacg
       61 agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga gagacaattt
      121 ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta caaacgtctt
      181 ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc ttatctagat
      241 tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt attttcgttt
      301 tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg gtattgcata
      361 tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag ttcttgaaag
      421 tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt gatgcaatta
      481 aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta ttttctttta
      541 atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata taaatcatat
      601 gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa caacatagga
      661 atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag ttatctaaaa
      721 agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt caaatacaag
      781 gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa gagggaatga
      841 aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag tatctcatga
      901 actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg ttgaaagtta
      961 tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg taaaattctg
     1021 tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa ttcgcaagaa
     1081 ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa aagaaaaagt
     1141 tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat tttagctatt
     1201 gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt caaacaaaaa
     1261 acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact tttttcatta
     1321 acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga ataagtgatt
     1381 tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt tatgatacca
     1441 tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt ccatacatat
     1501 acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt actatattcc
     1561 attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg taagatgtag
     1621 cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta cgtcaaactt
     1681 tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa tcgttcgaca
     1741 aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg gtttagctta
     1801 tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc gttttcagta
     1861 actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt tacccctgtt
     1921 aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt ttcctgaaca
     1981 tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc ttctaaagta
     2041 ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg ttttgtctga
     2101 acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa aaaaactgca
     2161 aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc ttgtgggata
     2221 aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac aatgatataa
     2281 aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa aagcgtcata
     2341 gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat actttgtgcc
     2401 aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc aatcacaata
     2461 aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc ccgaatggct
     2521 gctcgaaaag gacgatcccg tagaatcatt tccggtaaag caaaaatcag tcgaatccca
     2581 atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca acgattagca
     2641 aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc agaattaaaa
     2701 cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa aaaagtaatg
     2761 ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa agagattggt
     2821 tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa ataaactaac
     2881 agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg ttgagaaaaa
     2941 attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa aataaaacgg
     3001 gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat cagcaagaca
     3061 tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa gctacgtttc
     3121 aaataattca tcctatcacc tgctcttcac attatagcgc aaatctaatg aaaaaggaaa
     3181 gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca atgactaatt
     3241 ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata aatcaaggcc
     3301 atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt atatcgtttc
     3361 actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc aactggaaga
     3421 aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca gccaatatat
     3481 ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata acgacgccct
     3541 agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat aaatccagca
     3601 taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat gattggaata
     3661 actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat ggaatcacct
     3721 actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa ataaatcgtc
     3781 agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt tcgagaggca
     3841 gcaatattca ttattcatca actccttaga ggttctttgt taactatact ttactagaaa
     3901 agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt aagttcatca
     3961 taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta ataacttgtt
     4021 ttttt
//
LOCUS       DACCIQ010000144         3915 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCIQ010000144 DACCIQ010000000
VERSION     DACCIQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.149"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(25..1530)
                     /locus_tag="IVM62_002675"
     CDS             complement(25..1530)
                     /locus_tag="IVM62_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6846582.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="IVM62_002676"
     CDS             complement(1527..2213)
                     /locus_tag="IVM62_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6846583.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IVM62_002677"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IVM62_002677"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP6846584.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1320 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atatt
//
LOCUS       DACCIQ010000145         3847 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCIQ010000145 DACCIQ010000000
VERSION     DACCIQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.150"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            81..1595
                     /locus_tag="IVM62_002678"
     CDS             81..1595
                     /locus_tag="IVM62_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP6846585.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(1998..2981)
                     /locus_tag="IVM62_002679"
     CDS             complement(1998..2981)
                     /locus_tag="IVM62_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP6846586.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(2986..3633)
                     /locus_tag="IVM62_002680"
     CDS             complement(2986..3633)
                     /locus_tag="IVM62_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6846587.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(3718..>3847)
                     /locus_tag="IVM62_002681"
     CDS             complement(3718..>3847)
                     /locus_tag="IVM62_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6846588.1"
                     /translation="VYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
BASE COUNT         1224 a          731 c          829 g         1063 t
ORIGIN      
        1 ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta atatagtata
       61 aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca gccgttgcac
      121 ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca agttctagta
      181 cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca gatccaaaag
      241 aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg atggcagcag
      301 ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg cctgttgccg
      361 gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag
      421 aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa ggtggtggag
      481 gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat gcggtcgatt
      541 ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg atgagtgaaa
      601 agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg gaggggttat
      661 tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg acagacatta
      721 agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag
      781 aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc aaagaattta
      841 ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa ggatcaactg
      901 gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg gataaaatac
      961 aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt gttcgcggag
     1021 aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg gatacaaggg
     1081 ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg atttttgacc
     1141 aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga tatgtcacaa
     1201 ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa acactagcaa
     1261 aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg
     1321 ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag
     1381 gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag
     1441 aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc ttacatggaa
     1501 acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga caagcgggca
     1561 tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt
     1621 gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg tttagtgaag
     1681 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct
     1741 tatgttttta acttggttgg acttattacc gcagatgcaa gcttttgaac accgtttatt
     1801 cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     1861 aaacggcgga agcagaagaa attccttcgg aaataagctg aaattcacaa aaatttgaaa
     1921 agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
     1981 ttttgatcag catgatttta ttcagttatc ttcaaaatga tttttccgat gtttttgttt
     2041 gcttccatat attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt
     2101 ggccgaatct tgccattttc tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc
     2161 tctgctttat actgatcact tctaggtgtc aataatgtac caagcagttg gacacgtttc
     2221 ccaatcaata caccaaggtc tatctcaggc acgatggtcc cacctagcat accaatcaag
     2281 acccaacgtc catcaacggc tatagaagct aaattctttt cccagtaaga agcgccgatg
     2341 aaatccaaaa taacatcgac tccccgacct tcagtgattt ttcgtacctc ttcttgaaag
     2401 tcttgttttt tatagttgat caattcatct gcaccaagtt ctttacaaaa ggcgagtttt
     2461 tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc taattggatc
     2521 gctgctgttc ccacaccact tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat
     2581 tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat tccagcagca
     2641 gaaacatagt ctagcgtatc agggagaagg attgcacgat cggcaggcat cacagcatat
     2701 tcggcataac cacctagatt gactaatccg tatacttttg tcccaggagt aaaagtggag
     2761 atatcacttt tattctcaac tacctctcca gctacttcga ctcccaaaat agggtaggga
     2821 ggtttcaaag agactgattc tcttttcata atatctgttc gattgacagc tgctgtatgg
     2881 actttgatca gtaattcgcc ttgttttggt ttaggaacag gcacttcaat ttgttctaat
     2941 acatctacag aaccaggatg cttgattgaa atggcacgca tggatctatt cctcctttgc
     3001 ccattcttgg aatgctgtat tcaactcttt gctaccttca gaaagtacta gagtcgtacg
     3061 agaaatatcc gtttcttcca ctagagcggc aagtacagca gcaactgtag agcgagtgat
     3121 cgtttttgat gccttctcat taggcttagt taattgaatg gaagtaactt cttcatcgtt
     3181 agtcaatgtg actggttgaa gaatgacaaa atctaagtta gttcgtttca gccattcatc
     3241 tgcataatgt ttagtaatat aataatctgt catggactca ggccattttg ctggttcatc
     3301 tgcataaaca gcactgacca tcagatagcg agagacatgc gcattttcag ctgcgatcac
     3361 tgttttgatt gcgccatcca agtctacttg cagaagattc tttccttgag agccggcagc
     3421 aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg accaagttaa
     3481 atcaaaagag acatacgaaa cattctcttt ttttactaat gtttgggaag caacatctct
     3541 cacccctgca aaaatttcat gggaacttgg agctaaatct tttatcaggt gtcggccaat
     3601 ttgtccgttt gcacctacaa caaaaatttt catttggatc atcctttcga cgtcaaatct
     3661 ttccattcaa tagtaacacc aaaagagaaa aaaatcagag atcggctacg atgactatca
     3721 gttagtaaga gaagggtgct tgaagtatct taacaagaca ttgcatactg ctgcagtcgc
     3781 aagacctgcg acgattcctt caggtatacc acttgtaaaa ataaccgcta gaatagctga
     3841 ataaaca
//
LOCUS       DACCIQ010000146         3809 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCIQ010000146 DACCIQ010000000
VERSION     DACCIQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.152"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            123..941
                     /locus_tag="IVM62_002682"
     CDS             123..941
                     /locus_tag="IVM62_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6846589.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            945..1241
                     /locus_tag="IVM62_002683"
     CDS             945..1241
                     /locus_tag="IVM62_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846590.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1423..1776)
                     /locus_tag="IVM62_002684"
     CDS             complement(1423..1776)
                     /locus_tag="IVM62_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846591.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1796..2851)
                     /locus_tag="IVM62_002685"
     CDS             complement(1796..2851)
                     /locus_tag="IVM62_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP6846592.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            3067..3465
                     /locus_tag="IVM62_002686"
     CDS             3067..3465
                     /locus_tag="IVM62_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846593.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT         1287 a          548 c          690 g         1284 t
ORIGIN      
        1 gtttatgttg tagaatataa aagcaaatat aaatataaac acgtttatgt atataaacat
       61 atacataaac gtgtttatag tgtataatgg agttacaaaa ggttaataag ggaggtttaa
      121 tcatggaaaa gaagttgtca atcattactg gaaacattga aaaaggtgga acaggtaaaa
      181 caacaacaat ctacaatcta ggtggatatg ctgtcgaaaa tttaggtatg aaggtgctgt
      241 atattgatga agataagtcg caaaacctat caaaacgatt tcctgatgct ttcagtaaag
      301 cgaaaagaga gaatacaatc aatcatttat atcttcatgg atcagctgaa cctttagctg
      361 taaatgagaa catggatatt ttagtagcag gtgaagattt aaaacaagtt gagatgcaaa
      421 tgcgtgatat gcctaacaac agaaccctac tgtttagatg gatggttgtt aactatgaac
      481 agcttaaaga gaagtatgat ctaattctga ttgatactca taatgatgca ggtctactag
      541 ctcaaaatgc ttgggctgta tctgatcttg tcgtaggcat ttctgatcca tctaaagatg
      601 gttttgaagc tttgctgaag cttggtaaag atattgatgc actagcaaaa cagttgattg
      661 atattaagac tggtgaaaca ctgatggaaa ctaaatacgc tacgatcggt agcaggatca
      721 aacataatac taattcaagt cgtgagttcc tagaactggt accaacgtta tctaattatc
      781 ttggatatgt acaagagaag gaattgctta atgcaactag cttagaaggt aagaatattg
      841 ctgagttcgc taaggataag aatatctatc gtaagaataa ggatttcttt gaaagcacgt
      901 ttacgctata caacaaaata attgatacgg cacaagaata aggagtgatt gaagtgaaag
      961 aaaatagatt taaaaaattc gctgaagctg aagtagaaga agttcctgaa gtggttgaag
     1021 cagttgaaga acctaaacaa acacctaaag aaacaacaag ttctttggta gctaaactaa
     1081 aaagtgatag ttctgaaaag aagaaaaatt atcaattcac gcttcaacca tcgcaaagaa
     1141 agaaactatc taaaatcagc gaaaagttag gaattcgatc agattcagct ttacttggtg
     1201 aaatgataga taacttgtat gatgaagttt ttggtgagtg atcagtttaa agctgatcac
     1261 tttttttatt ggaataagcg gttaggtata gcttaactaa atttaatata cgtgtttatg
     1321 gatatatacg tatacatgtt tatatacatg tatacgtata tgtttatgtt tatatttgta
     1381 cataataaaa aggctaggta ttgtaacctc gcctttttat ttttaaagta agtctttgtt
     1441 acttggtgca ttgaaccctt tcagcattgg cttttctttc tttgctgaat gatcggatgt
     1501 aaccgttgaa ggatgctctt gttgaatagg gttatgatca gttggtttat tctttacttc
     1561 tgctactttg ctatcgtttc tatcgttgcc tgcttcaata attgtaactt cattgttctt
     1621 tacacgccga tataggttca aagctttttt gatcgtctga gagtatttaa cgcctagtgt
     1681 ttcatattcg gattcaagaa actctgctaa aggtaactcg ctaacctttt ctctattgat
     1741 tataactaca taacgctctg attttccctt attcatgttg atctccttcg ctgttttatt
     1801 gagttaaacc aaacttgtaa aagcctttta cattagaaaa ttcactgtct tcaacaaaca
     1861 aggtatgcgg aattttatct tttacaagtt gtttgtcgat aatagaagct ccaccaccag
     1921 taaagacaac tttatctaag gttgtgaagt ttttgaatgt agctaaaact tctgcaacaa
     1981 ccatattggt gtatgttttt atttctcttt caactacttc tgaaatatca aaactttcat
     2041 ttccgcttgg cttaaataga tatacgcctt tttctttgcc ttctcggata atcttttcaa
     2101 gttggaaagc attaggactg atcggaaggt caagcatatt tacgatacga ttatataaag
     2161 taaaaatgcc ggtatccttt tgttcacgat tgttttcgtc aatttcaaaa ttttggacaa
     2221 catcaaaaag tttcgtgcca ccgccaatat caacaaaacc gatcttctga ctgatcaatg
     2281 tttcatcttg tacactacca tcatcagcaa gtaggaggtt atatagtgtt ccaatcggct
     2341 gtggcagtac ccaaacggta tctacgttta tattataagc tttaccgtca acttcaactg
     2401 aatgttgtcg tgtggcttgt tgcatcacgt atttgacgat tgtttcggta tagtcggatg
     2461 aaggcagacc taaaactaga ttagcggtta aaacgtcatc agattgatct tctaaacgtg
     2521 ctaggttagc taaagagaaa gataggatat tttggtaggt ttcctgcttg tatctgctga
     2581 tcccaaaacc tagtgattct atcacgtagt catcttttcc tagctcgtgt atatccttcc
     2641 caaagtaata gctttcagta gtgtttacta ggctatattc agaaacgttt agtgatcgtt
     2701 ttaatgagga acctatatta gaaaaacgtc ctgaattaat gatatttttg ttcaaaatac
     2761 gagagggaag tgagagtgga gaaacgtgtg tactcatttc ttttactgat ttattcccta
     2821 agtcaacgga aaacaataat ggttctttca tggtatgacc tctttctgtt tgtgtttatg
     2881 gtatttcggt acgaaaatag tttacaccta tttactatta gttgcaactg tttataagaa
     2941 aaaaatgcaa tcataaaagt ctaattaatt ataataaagt gtaaataaaa aggtgtaaat
     3001 aaatgcttgc aaatttatct tattatgata ttataaaaac ataaattata ttaaggagtg
     3061 atcctgatgc gattgagaag aaataagaat tacactacca ttattttaga agattttact
     3121 gatcaagaat tacttattgt cggtaaaaca aaagcagatt ttatcgaaaa gggaaatgct
     3181 tcaatagtaa aagcttcaaa gattgctgtc gaaaaatatt ttgattcttt agaagaaagt
     3241 gctaaggaaa tttttaatgg agagaaagct ggtagctatg agttagacgg tattagattt
     3301 agcttgatcc caacaactga aaatattatt ccgaagaaat tatattatat cttttcatct
     3361 gaaaaacata cttcaatgaa gccttgtatt tttgattggc aatggttgat tgatgctgta
     3421 ttggatgtaa aatgttcaaa atacaatatt actttggggt gttaattatg agtatgctag
     3481 ttagaaaatt accattatct gtaagagaat ttctatttta cttggtctgc ttaatcgcat
     3541 cagggtttaa gctgatcgaa catattttta tctttgctta tgtattagca atgatcgcat
     3601 ttttatctgg cggttggtta attatttatt tgttaagcta agttttagat caaaaataat
     3661 taataaatta aacaataaaa taaccgttaa taaatgtaat gaaaggacaa cgcctaacaa
     3721 tcagcttgta ataagattat aaaataacat tatattaaaa taagtgcttt ttaaaggaca
     3781 aattaagtga atcaacataa acgtgtata
//
LOCUS       DACCIQ010000147         3716 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCIQ010000147 DACCIQ010000000
VERSION     DACCIQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.153"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..692)
                     /locus_tag="IVM62_002687"
     CDS             complement(<1..692)
                     /locus_tag="IVM62_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6846594.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLA"
     gene            853..1542
                     /locus_tag="IVM62_002688"
     CDS             853..1542
                     /locus_tag="IVM62_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6846595.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1556..3058
                     /locus_tag="IVM62_002689"
     CDS             1556..3058
                     /locus_tag="IVM62_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6846596.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3071..3547
                     /locus_tag="IVM62_002690"
     CDS             3071..3547
                     /locus_tag="IVM62_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6846597.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1218 a          528 c          675 g         1295 t
ORIGIN      
        1 gctaaagatc caccattaag aaattcttca atggctgttt gtaaaactaa gtctgcagac
       61 tttcctatag gtgaatgaat atagttagtt atagcaataa tttttgcgcc atttttttta
      121 gctattttca atgaatcata agtatctttc gttttcccag ataaagaaaa aatgattact
      181 aaatcattaa ctgttaataa agaagcgact tgggcttgaa aatgagggtc tagtacagct
      241 tttgcttgaa ctcctacgcg aagaaacata ttttctaaat caagactagt atttccacta
      301 gatcccactc caaatatata tatgtttttt gattgagtga tcattttaat ggcttcattc
      361 aatttttctt cgtctaaaag cctaatagtg gaatagagag cttctgttaa actattagca
      421 atttcatcat agtactttac actagtcgga tgttcttttt tttgactaaa gtcttccttt
      481 gcaatttcaa tttttaaatc agaaaaacca gaaaaaccaa ttttttgaca aaagcgaata
      541 atagtagcat cgccaacttt tgtgtttttc ttaatatcac tcatcgtact ataaataata
      601 gttttcccag aatttaaaat aaaatcagcg actttttttt cagacttcgt taaaaatgga
      661 taattttctt taattaaata gatataactc atacaaactc ctctacaatt tcaacttttc
      721 tccattatac tccaaaataa taaaaaaaac tcctaaatac tccattttta tctttacttt
      781 tatgaaagcg gatgctatat tgtttttgga gttgttatcc aaaaataata aaaaattgga
      841 gtgtttagga taatggataa aaagaggttt ttagatctta tttcaaaagg tttgattgtt
      901 tcctgtcaag cattacctgg tgaaccattt tacaaagagg aagaaagttt aatgccttat
      961 tttgctttgg ctgctcaaag ggctggagca gttggtatac gtgcaaattc tgttcgagat
     1021 attattgaaa ttagaaagtg tgtatcatta ccaatgattg gaattattaa aagagattat
     1081 ccaccagaat ctccattcat tactgccact atgagagaag tagatgaatt agtagaaacc
     1141 ggtgttgagg taatcgctct tgattgcact cttagaaatc gacatgatgg actatctata
     1201 aattctttta ttactaaaat aaaagaaaaa tatcctcatc agttatttat ggcagatatt
     1261 tctacatttg aagaaggcaa aaatgcatat aaagcaggaa tagattttgt aggaactact
     1321 ttaagtggat atactgagga aagtaaaaaa caagatgggc cagatataga tttgattaaa
     1381 aaattggtag aagaagggat ttgtgtaatt gctgagggaa ggattcatac tccagaacaa
     1441 gcaaaagaga taaaaaaaat aggagttgca ggtattgttg tgggtggtgc tattactcga
     1501 ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt agggggagaa ataaaatgtt
     1561 taaaaaattt tcacaattag gacgcgcttt catgttacca attgctatat taccagtagc
     1621 cggattacta ttgggactgg gaggagcgtt aacaaatgag agtgctataa atgcttatcc
     1681 atttttagat caaccgtggt tgcatacaat tttaagtatt atgagttatg ctggaaatgc
     1741 agtttttgca aacctagcac taatttttgc aattggtatt gctgtaggac ttgcaaatgg
     1801 agataaagga acagctggtt tagctggtgg agtagcttat ctagtatata ctgcaactat
     1861 tagtgggttt cttgaacttt tttcagcaaa agatgcaaca ctagatacag gtgtagtggg
     1921 atcaattgtt gttggtggta ctgttgcatt tttacacaat cgttatagaa aaattgaatt
     1981 accacaattc ttaggttttt ttggaggatc aagatttatt ccgattgttt ctagtttagc
     2041 tgcaatcatt attggttctt tcttttattt aatttggcca ccaattcaaa atggtttagt
     2101 agttgctggt gaacaaattg cccaaatggg tagtttagga acttttttat atggtttttt
     2161 attgcgttta acaggtgctg ttggactaca ccacaccatt taccctttgt tctggtatac
     2221 atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc aaaatatttt
     2281 ctttgcccaa ctagctgatc caaaccacac tggtctgttt acttatggta ctagattttt
     2341 tgcaggaaga tttgcaacaa tgatgtttgg attaccagca gcatgttatg caatgtatcg
     2401 tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg gggcccttac
     2461 atcattttta actggaatta ctgaaccagt tgaatatatg tttttatttg ttgctccttg
     2521 gctttatgtg attcatgcat tcttagatgg attatctttt tattttgctg atatcttaaa
     2581 tattcgtgta gggaattcct tttcaggtgg tttgatcgac tttttattat tcggtgtact
     2641 tcaagggaat gacaaaacta attggttaaa attaattcct tttggtattg catgggcatt
     2701 tatatatttc tttgttttta cattctttat caaaaaattt aaggtagcta ttcctggaat
     2761 ggaagaagaa tcagaagttc attctgaaaa agtaataaac aaaaatacat cattacatga
     2821 agagtctctt atcattattg aagctcttgg tggaaaagaa aatattgagt ctgttgaagc
     2881 ttgtgcaact cgtttgcgtg ttgctgttac gaatggggag attattcaaa aacaaacaat
     2941 aaaagacact ggtgcaactg ctgtcttcga agtgaagggt ggcatccaag ctgtttttgg
     3001 agggaaagct gacttattaa gtcaagaaat caatcaaata ttaggaaaag ataattagga
     3061 ggtttttaat atgtttgaaa ttttcaaaaa gaaaaaacaa gaaacttttt atgcaccttg
     3121 ttccggaaaa atcatttcca tcacagaagt aaatgatcct gtcttttcac aaaaattact
     3181 aggtgatggt tttgccgtca tacctgatgg ttcaaaaatt tatgctccat tggctggtcg
     3241 agtaacaaat atatttccta ctaaacatgc tattaatata aaaagcaact caggcattga
     3301 ttatttgatt catattggaa ttgatacagt tgaattacaa ggagagcctt tttcgatttg
     3361 tgttcaagaa aatgatgagg ttactcctga aacctcgttg gttgatgtgg attttgacaa
     3421 aataataaaa tctggtaaag atacttctgt tatagttgtt tttccggaga aaaatcagtt
     3481 caatgattta ataatagata atggtcgaat tgctcatggg aattcatgtg gaaaaatcac
     3541 tttttaaaaa agtgtatata aaaagctata aaataagcta aataggatat ttgtcaaatg
     3601 aagttggtga acagaaagaa ctgttttctt tctgttcatt tttttatttt attagtaatg
     3661 attccccatg ccaaatcatg atccgtaatt taaagttata aaagcttcga aatcca
//
LOCUS       DACCIQ010000148         3432 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCIQ010000148 DACCIQ010000000
VERSION     DACCIQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.155"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            188..1615
                     /locus_tag="IVM62_002691"
     CDS             188..1615
                     /locus_tag="IVM62_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP6846598.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(1637..2443)
                     /locus_tag="IVM62_002692"
     CDS             complement(1637..2443)
                     /locus_tag="IVM62_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846599.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(2531..3331)
                     /locus_tag="IVM62_002693"
     CDS             complement(2531..3331)
                     /locus_tag="IVM62_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846600.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
BASE COUNT         1064 a          651 c          490 g         1227 t
ORIGIN      
        1 aaaaattatc aagttaaaaa aataaatttt gaaccaaatt atttttaata aaatagctag
       61 tttttttatc aaaaaaaata gagcttttca gctctattca gactgtagac aaactcctaa
      121 atttggagtg ttgtttgcag ttttttgttt tatactaatt gtataaacta ttctgggagt
      181 gattctaatg atgagaaaac aatcgattga aggtagaaat caatttgcta tgttaacaat
      241 tgatgatctt gtacctaaag atcacttagt tcgaaaaatt gatgctgcga ttcagtttga
      301 tttcatttat cccattgtag aatctacgta ttcaacctat ggtcgtccca gtattgatcc
      361 agtggtctta attaaattag tatttatcca atatttattt ggtatccgtt cgatgagaca
      421 gacaattaaa gacgttgaaa caaatgttgc ttaccgttgg tttttaggtt attcattcga
      481 ggatcctatc ccacacttct ctacttttgg taaaaactat gttcgaagat ttagagaaac
      541 aactttattt gaagatattt tctctcatat tctagaacaa gctgtaaaag ctggcttcgt
      601 tacggaagac aacctttata ttgattctac tcatatcaaa gcaaatgcca ataaacataa
      661 gttcacgaaa gagatgacct acggtcaagc aaaagcttat caagatgaat tagaagatga
      721 aataaataaa gaacgtattg ctgtaggaaa acgtcctttt acatgggaca cagaaagtga
      781 gttgagtctt caaaaaataa gtcattcaga tccagaaagt ggttactatg ttaaaggtga
      841 acgagaaaaa caatttgctt attctgcaca tacttcctgt gaagataacg gttttatctt
      901 atctacatta atcacaccag gaaatgtgca tgacagccaa gtcgcaatcg atctggttaa
      961 acagtcgaaa agagcttttc caaatataaa gcgtgtggtt gctgatgcag gatataagac
     1021 gcctaaattt gttcattttc tgtcacactt gaaactctac ccgattcttc catatactgc
     1081 acctcatgga gtgaaaggaa tgtttcgtaa aaaagacttt gtttatgatg cctattttga
     1141 ctgctattta tgtcccaata atcaaccgtt gttattttca actatcactc gtgatggtta
     1201 tcgaacctat aaatcaaatc caaaattctg tgtgagttgt ccattacttg aaaattgtac
     1261 tttgagtaca aaacatcaaa aaatagtgac tcgacatatt tgggctgatc ttatggatga
     1321 acttgaacac caacgccata cggcgttgaa tagagaaatt tacaaaaaga gaaaacaaac
     1381 cattgaacga atatttgcag atgcaaaaga aaagcatggt atgcgttgga cgaaatatcg
     1441 aggtcttgaa aaagtcgcca cgcacacgat gcttacgttt gctgccatga atttaaaaaa
     1501 actagccaca tggctatgga aaacaaaaaa gcctatggct ttttatttca aaattatgca
     1561 aagactaaca aaaaggagtg gcttaattac caactccttt ttgtctacaa tctgaataga
     1621 gcttttcagc tctattttat tcaatatcat tttgatcaat taataatcgt acactccaat
     1681 taaccgagtt ttgatcactc aaatatttat aatctttgtc atcaaaaacc agttgccaaa
     1741 ctctggtacg aacagtttct tcgtccactt cctcatctga agagtattgc gataatgctt
     1801 cttcaactat atactttaca acttcctcaa aatcagtagg aactttatca ataaccactt
     1861 catagtcttc ttgttttacg atattgggat caatatgttc atccaaacat acctcagtga
     1921 gtttttcttt cacttctgta tctctttctg tttttaggta ctctgcatag tcttccttag
     1981 accattgttt ttcaagatca tccgctatac ttttaataaa ggattcttta tttaaccaat
     2041 tatctgcggt atctatacct tcaaactcaa aagctgttcc ttctatacgt gatttgacga
     2101 aagcgtcaat tttcttgtat tgattattca tttcccatga aacacactca tcttcttgcg
     2161 taatatcata aattttttcg gaaaaactaa ctaattcatt ccacgattta tcctcggcaa
     2221 cttgacttag tttcgatgca ataaaaggaa attttgcttt taagtcatcc atattcttga
     2281 tctcaaattc gatattttct tctcctacag ctgaaatcca tgctgaaaga ttgattgcaa
     2341 tatcgctaaa gtcttctaca ttttcgacat ttcctgtatt atcttttatt actgcttcta
     2401 aagccatatc ggttgttgca ctcatggttt gatctcctct cataatagag caaaaagccg
     2461 gtttaaccga ctttttgcat ggtacgtttt gtttggtagt tttctgcttg tgtttatgtt
     2521 gaaataatcc ctatagctct tcttttaata gttcttcaac tttatctctt aactctccct
     2581 gaccactctc taatagattt aaatagctca tcagaatatc cgtttcatac gtttttaatt
     2641 cagctctata ctcactttct gaatatgatc gtaaataccc atcattgcta aatgcaacta
     2701 gatcatcttg aaagttccat tctcctaaat ttgttttact tactgcttcc catggactat
     2761 tactaaacat ttcattaaag aaatcttcgt cattttgata taccttcaat tcaggaaaat
     2821 agttgctgtc ttcattgatt tcttgcacca tttttccaag ttcttcttca gataatttac
     2881 cttccaataa ttttactttt tctctgtcat ttgcataagc catttgaacc tctccttttt
     2941 ctttttcaat ttcttcaggt gtttgttttg gcgtaacttt caaatctatt ccattgccta
     3001 ttagacctat tagataacca tagaacgttt taaagtcact tgtagcaaga gatttggcat
     3061 cagggtatgt atcccctcca attttatcta aaacaaaatc agcaatcgtt acccaaggag
     3121 aatatatcgt acatactaca tcccatgtag catcttcaat attcgtccat gtcggaaagt
     3181 cattttcatt caaatagtct tttttcattg tttcgatcag agtttttcca ctttcgtttt
     3241 ggcttagata ttcaacaaat tctgcaccat caaacctgct actaaatagt ttagctaatg
     3301 agtctaaagc tgtttttgat actcccacca ttactacacc tcctattaat attttacgat
     3361 gacttgtttt tcattcacaa ataagatttt ctacatctta tctattgctt tatagctttt
     3421 ccatacatta tt
//
LOCUS       DACCIQ010000149         3334 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCIQ010000149 DACCIQ010000000
VERSION     DACCIQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.156"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(451..1761)
                     /locus_tag="IVM62_002694"
     CDS             complement(451..1761)
                     /locus_tag="IVM62_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6846601.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IVM62_002695"
     CDS             complement(1773..3191)
                     /locus_tag="IVM62_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6846602.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1032 a          676 c          514 g         1112 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataa
//
LOCUS       DACCIQ010000150         3282 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCIQ010000150 DACCIQ010000000
VERSION     DACCIQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.157"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            1..585
                     /locus_tag="IVM62_002696"
     CDS             1..585
                     /locus_tag="IVM62_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6846603.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(662..1837)
                     /locus_tag="IVM62_002697"
     CDS             complement(662..1837)
                     /locus_tag="IVM62_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6846604.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1919..2299
                     /locus_tag="IVM62_002698"
     CDS             1919..2299
                     /locus_tag="IVM62_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP6846605.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2373..2735
                     /locus_tag="IVM62_002699"
     CDS             2373..2735
                     /locus_tag="IVM62_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846606.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1114 a          539 c          622 g         1007 t
ORIGIN      
        1 atggcattcc cacaactaga tttaaaaagt gaaaaaggac caaaagctgt tatcaaaaca
       61 aaccgtggtg acattactgt tcaattattc cctgaactag caccgaagac ggttcaaaat
      121 ttcattgaac tttccaaaaa aggttattat gatggcgtga tttttcaccg cgttattcct
      181 gatttcatga tccaaggcgg cgatccaact ggaacaggaa tgggtggcga aagtatttat
      241 ggcgaaagtt ttgaagatga attcagtcgt gaactgttca accttcgcgg agctttatct
      301 atggcaaatt caggtccaaa cacaaacgga agccaattct ttattgtaaa taacacaaat
      361 gtgccagcta atatgcttgg acaactagaa ggtgctggat tcccatcaga aatcattgaa
      421 gcgtataaag gtggcggaac tccttggcta gatttccgac atacagtctt tggacatgta
      481 ctagaaggga tggacacagt agatgaaatc gcaaatgtac aacgtggtcc acaagaccga
      541 cctgtacatg atgtagtgat tgaaacaatt gaaatttcag aatagtttgg tatggtacgg
      601 agtaattatg aaaaaggacg aaacctagta aggtctcgtc cttttatttt gaataaatga
      661 tttaatcgat tgtctctaaa gcatacttga ttcgattaag tccttccagt aaagtttctt
      721 ctggacaagc aatattcaag cgcatatgcc catgaccgga aggaccaaac gtgatgccag
      781 gatttaatac gacttttcct ttccgaacaa gagtgttctc caattcttgg tcgtctgaaa
      841 tataggcggt aaaatctaac cacattaagt aagttccttc agggttcatt attctcactt
      901 tcggaagata ctgttcaaaa aactgattaa ctgtttgaac atttttttct agataaggaa
      961 tcagttggct taaccattct ccacccgtct cataagccgc ttgggttcca atcagtccaa
     1021 aagtgttgat ttcgtgctgt tggttcatca ctaaatgttt ttcaaaatca gctttcatct
     1081 ctggattttt gataaatacc attgaatttt tgattgcagc tagattgaaa gtcttggttg
     1141 cggaagtaaa agcaactagt agatcatcta aattaggatc aatcgttagc atggaagtaa
     1201 atgtatgatc gaataaagtt aaatcttgat ggatctcgtc actaaacaga atcacttgat
     1261 gttttaaaca caattcgctt aactgtttca attcttcttt gctccaaaca cgccctccag
     1321 gattgtgagg attgcataaa atcattgctt ttacattgtt ttcttcgatt tttttctcca
     1381 tgtcagacaa gtccatgatg aaatgattat ttttttctag caaagagctt cgaacgatgt
     1441 gacgttgatt tgtttctata atagaggaaa atggaggata aactgggtca tgaatcaaaa
     1501 tagaatcgcc tggttttgta aaagtttgta ctgctgcagc taaactggtt aacacaccac
     1561 ttgtaaaaac gatattttct ttcgtcaact ccacagcatg gtgttggcgt tcccaatgaa
     1621 tgatagcctg atatagttta tcagacagag tggcataacc aaagattcca tgtttgatgt
     1681 attcactaaa cgctttagct accccaccgg gagacaagaa gtccatatct gctacccaca
     1741 aaggaagtaa gtcatccatt tgatatgtct cagcaattgc gtcccatttt acactttctg
     1801 tatcttttct tgaaatctgt ttatcaaatt cgatcatctt aagacctcca gtcattttcg
     1861 tcgcttcttt ttttgagcta tttacagtat aatgataaat gcgaagagag ggaagtagat
     1921 gacttataaa attggacaaa taattgaagg aaaagtgaca gggatacagc cttatggcgc
     1981 attcgtttct cttgataacg agacgcaagg acttattcat gtttcagaag tacaatccgg
     2041 ttatacgaaa aatatccatt cattgttaaa agtcggacag ccagtaacag ttcagatcat
     2101 cgacattgat gaatattcca aaaaaatcag tttatcttta agaacgttag aaaaaacaaa
     2161 tccagctgtt ccatatcgca gaaaaaggta tttcacgaat aaaaatcgta agatcggatt
     2221 taccactatt gcggagcagt tacctatttg gatagaagaa gcattatctg aactagaacg
     2281 gaaagaaaat aagaaataaa aagtgtattt tttctgaaaa tgtgctagaa tttttatgta
     2341 agccatagaa caagttgatc gaggtggaaa ttttggcaag caaaagagtt gctggtacaa
     2401 ttatgttgca tttggaggat ggtactaaaa aatttttggt ccattcaatt gatgacaagt
     2461 tggaatttgc gttagcggaa ctttctgaag gaaagactgg tttagcgaat atacttaatt
     2521 tcttaaaaga aatagttcat atcgatgtta gcaagattag tttgatggaa ttgacaaata
     2581 cacatattaa agaggaaaat gttccgttgt ttgtctttga aactgaacaa aagaatcaaa
     2641 gagaagaatt aggcgaagga tatatttggg aagaaccagg tcaaatgcgc tctgttcttg
     2701 gaacttacga agtagaggga gtgcctttct tctaaaatac agattaagcg aacaaagaag
     2761 tttgaatttt tttcaaactt ctttttttat tgtttggtcc aaaaaataca atgaaatgtt
     2821 ctgctaaaga tagtttttta gtgcatttta ttttcaaaat atgttgaaaa cggttgtata
     2881 acgaataata ataattgatc gaaaaatcat ttcacaaagt aatgcttaat ttatagttga
     2941 ccaaggaaga aatacatgat aaatttaaca gcgttgattc tgaagtggat gttatatcac
     3001 atttctaaaa aatttaaaaa atagttattg acatgttact aacgacatga taagataact
     3061 gagtcgttaa aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac ttcaaaaaag
     3121 ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaaga tttcgaaaaa
     3181 cgaaatcgaa aaaaactttt aaaaaagagt tgacaatcgc ttgtctctct gatatgatat
     3241 aaaagttgct acggcagcca aaaacgaatc gggaaacact cc
//
LOCUS       DACCIQ010000151         3221 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCIQ010000151 DACCIQ010000000
VERSION     DACCIQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.158"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            38..346
                     /locus_tag="IVM62_002700"
     CDS             38..346
                     /locus_tag="IVM62_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846607.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            350..712
                     /locus_tag="IVM62_002701"
     CDS             350..712
                     /locus_tag="IVM62_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846608.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            934..2340
                     /locus_tag="IVM62_002702"
     CDS             934..2340
                     /locus_tag="IVM62_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6846609.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            2790..>3221
                     /locus_tag="IVM62_002703"
     CDS             2790..>3221
                     /locus_tag="IVM62_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6846610.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIAC"
BASE COUNT         1130 a          462 c          719 g          910 t
ORIGIN      
        1 atcgaaaatg gtggcaattc tggaagtgag gtgtgacatg gataaaattg atttgagaga
       61 agaaacagtc gatagaattg aattgatgta tgaacggtta gctgattatt tgaatttgcc
      121 ggatgaagat aattatagtg aaatgcttga acgaatgatg gatgttctca actcggttat
      181 caatgctgat ctagcaaaat cttcagtttt gactaatgaa tttgttcata agcatttagc
      241 gcaaacaact gatgaatcag aaaaagaatt ttggttgaaa ttaatgagtc atgttatgca
      301 gttaaaccaa atttccgtta ttaattccaa agaaaaggag atgtagttta tgggaatgac
      361 tttagcagat attaaagcgc aagaagaaaa attaaaaaaa gaacaagcga aacttcgcca
      421 acagaaaaaa cgcttgattg agaaagaaaa tgctgcaatg ggcaaggctc ttcgcaagcg
      481 ttttgggttt gaaacttttg ctgattttga gcggtggcta tcgtcgattt ctgacgatca
      541 gcaacacgaa aaatcagtgg ataatgagtg gcttgctgat gaaaaatcag cgccaagcga
      601 acgagattat cccgatgaat ctgacggctt attcggagtg ataccgaata agaaaagtca
      661 gatgagtgag agtaaattta cgtcaatatt taatgacgtg aatttacgat aagcgattat
      721 tttgcgtgaa gcaaaataaa gcgtaaaagg agttgagagc gtgacacgct ttcggctcct
      781 tttttcgttg tcacttgtga cagcgaatac cctcggagag catttggaat tgtgacagcc
      841 atgtcacaat taggaaaatg ttactctttg acaaagagta acagaaaggt ttacactctt
      901 tttattagcg acagcgcgaa ggagggtttt aacttggggt ttagtatttc atttaaaaaa
      961 ggaacgaaga atacaagtat ttctcataat aatcgtgagt taactgatag ccagaaaacc
     1021 aatgattggc ataagcatat tgacttcacg aaatcggatg aaaatattta tttagagcag
     1081 acgaatctag cagataaata tgaggaattg tttggcgaag tggtagaaaa atataatgcg
     1141 aaacaaaaaa gagccgatag aaaaatcgag gactatttag cgaaagtgcg caaggataaa
     1201 aagttggaac ctcaacgaga atttattgtg caaataggca ttttagatga tttccagaca
     1261 aatcgagcag atggttcttc tactggcata tcgaaacaac aagctgaaca aaatcgagtg
     1321 attgccaata aaattttggt tcagtattac aaggagtttc aagagagaaa ccctaatttg
     1381 gctgtttata atgctgttat tcataatgac gaaataagtc cgcacctgca cttgaatatt
     1441 gtgccggttg ctgaaggtta taaacggggt gttcaaaagc agccgagctt taataaagca
     1501 ctggttcaac aagggataca cgctgataaa gataatggtc gagtgctttt caataatttt
     1561 agaaatcaag aagttgactc tatagaacga ttaatgaatg attacggttg gaaaagggat
     1621 attgtcggta ccaacaaaat taaagatatt cacgaataca aagaaatcat gagtgaaatt
     1681 tctgaattgc gccaaacggc tgtaaaagag cgtgagagcg tttctgatga gttgaggtat
     1741 atttataggc aaagagaaga aaacgagcgt gataaggctc tgatggcttc tgagagagcg
     1801 aagattgaag aagagaagaa aaatatgctg aatgtaaaaa aagagatggc ggtcattttc
     1861 caaaaagata tggttccttt agaaagtttc aagaggcttc ctgatggttc gtatcattta
     1921 tctgggaatg attttgttga tttgtatcaa cgagctacga gagcaacaaa tgccaagaga
     1981 aatgctgata acatgagtga gcaatattta cagatgcttg agcaaaatac agaattaaat
     2041 gagtatgtag ctacattgga agaagagcgt catttgtctg gtagaaaaca attagcagat
     2101 ttacaagtgg aaaataaaaa actgaaagaa gaaaatcgct ctttacgcca gcgtttagag
     2161 cgattaaaaa acgcatttca aaaatcgatc actcgtttgt ctatccgttt tggtcttgca
     2221 gaaaaggata tagggctttc tgaggaatta gaagtattag agcaggagaa aaccttattt
     2281 tatcgtcaaa acgtctcaca gcataaatta gagcgttctg aagatgagat cgagtggtag
     2341 ttacgatgtg cagtcaacgg taccggaggc ggtttgtgcc gaggataagg agttgacggc
     2401 taaaccacta gccttttaag ctgacatttg ctaaaaaatg gccagtccat tttttggcca
     2461 acctagcgtt agcgaagttt gggggcgccc ttttcaaagg gtgcggtttt atgtttttgg
     2521 atatttgcat agctaaagag aagtgctatt attgagttat caaataaata aactgaaaaa
     2581 agagcaaaaa aataaagtcg agcaacgcta ccaacgttct cgacctttac ggaaactaat
     2641 cgcactagct ccgtaacttc ttaatcatta tacgaatatt tttagatttc ttcaagacta
     2701 gagatttttt ggtaatgaat ttttaagagt tacgtgaact tttgaaaatt ctttccagaa
     2761 gtctcttttt ctttgaggag gaacaaaatt tgagtaaaaa tggcataatc gtaaatgagt
     2821 gggaaaaatt aacagcccta tctaacaggc ggttaaatgt acaaaatatg tacaccccct
     2881 taaaaatgca gtggtcaatt gaccgaatta ctatcgttgg aaaactgaag gaaaatattt
     2941 attatcacac tcaaaatgat gtgttgattt tagattttga gcaattaatg cgggtgaatg
     3001 aaggcaatgg ctatttaaaa gcggttggga ataacggctg gcaattatta gaccaatacg
     3061 aagaaaatat tgcttatatt gagattttga aatggcaaga tggcaaaggg cggattgatt
     3121 ttaacccgaa taagattggt cattttttag ctagttcaat gaaaaatttc attcatgatt
     3181 tatttctaga accacatttc tcacgtgcgg atattgcttg c
//
LOCUS       DACCIQ010000152         3178 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCIQ010000152 DACCIQ010000000
VERSION     DACCIQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.159"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..204)
                     /locus_tag="IVM62_002704"
     CDS             complement(<1..204)
                     /locus_tag="IVM62_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846611.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
     gene            complement(192..821)
                     /locus_tag="IVM62_002705"
     CDS             complement(192..821)
                     /locus_tag="IVM62_002705"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP6846612.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(828..1544)
                     /gene="pyrF"
                     /locus_tag="IVM62_002706"
     CDS             complement(828..1544)
                     /gene="pyrF"
                     /locus_tag="IVM62_002706"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP6846613.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1541..2473)
                     /locus_tag="IVM62_002707"
     CDS             complement(1541..2473)
                     /locus_tag="IVM62_002707"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP6846614.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2470..>3178)
                     /locus_tag="IVM62_002708"
     CDS             complement(2470..>3178)
                     /locus_tag="IVM62_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP6846615.1"
                     /translation="KGDLVKEMQQPGQFLHILVPRADLLLRRPISLNHIDHETNTCRI
                     IYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSNLHTGDEVFIIGGGIGVPPLYELS
                     KQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETRIATDDGTYGMHGNVGNLLLGTKT
                     EPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRMACGIGACYACVCHTPEDETGTKS
                     VKVCDEGPIFKIGEVII"
BASE COUNT          844 a          739 c          573 g         1022 t
ORIGIN      
        1 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
       61 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
      121 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
      181 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
      241 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
      301 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
      361 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
      421 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
      481 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
      541 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
      601 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
      661 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
      721 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
      781 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
      841 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
      901 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
      961 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     1021 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     1081 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     1141 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     1201 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     1261 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     1321 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     1381 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     1441 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     1501 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     1561 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     1621 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     1681 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     1741 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     1801 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     1861 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     1921 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     1981 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     2041 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     2101 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     2161 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     2221 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     2281 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     2341 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     2401 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     2461 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     2521 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     2581 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     2641 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     2701 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     2761 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     2821 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     2881 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     2941 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     3001 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     3061 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     3121 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttc
//
LOCUS       DACCIQ010000153         3133 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCIQ010000153 DACCIQ010000000
VERSION     DACCIQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.160"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            12..275
                     /locus_tag="IVM62_002709"
     CDS             12..275
                     /locus_tag="IVM62_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP6846616.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            269..619
                     /locus_tag="IVM62_002710"
     CDS             269..619
                     /locus_tag="IVM62_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6846617.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(643..1257)
                     /locus_tag="IVM62_002711"
     CDS             complement(643..1257)
                     /locus_tag="IVM62_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6846618.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1571..1993
                     /locus_tag="IVM62_002712"
     CDS             1571..1993
                     /locus_tag="IVM62_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846619.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2003..2206
                     /locus_tag="IVM62_002713"
     CDS             2003..2206
                     /locus_tag="IVM62_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846620.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2417..3001
                     /locus_tag="IVM62_002714"
     CDS             2417..3001
                     /locus_tag="IVM62_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6846621.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1082 a          465 c          573 g         1013 t
ORIGIN      
        1 aggtgtctac tatggaaatg ttacctataa aaaaatgggg aaatagtaat ggccttagat
       61 tgccaaagta tattatggaa tatcttggaa ttcatacaga agacaaagtg aagataatac
      121 aagaagaagt aaacggtcag aagcggctaa ttatcgaagc ggctaatata gaaaatgaat
      181 taacaattga acagttattt gaaaattaca ctgatgaaag aaatcatgtg acgattcaaa
      241 atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa cctagacaag gcgatattct
      301 attattgaat acagcacctc gatctggaca cgaacagaca ggtaaaagac cttatattgt
      361 tttaagtcat gatattattg cggattatag taacgttgtt acagttgctc caatttctag
      421 tacaactaga aactatccgc tatatgtaaa tataaactca gaatatgaaa tgaagacaac
      481 aggaaaagta ttattagatc aactaacgac aattgattat gaagccagag agtgtatttt
      541 tttggaaaaa gcgcatgata gtttagttga ggagttattg atgaaagtta gaacagtttt
      601 tcaaaaagta agcaaataaa atagaaacag cagcatgatc aactattcgt gattgaccat
      661 gctgtctttt tttattctat atacggtttg tctcgtaaca ttgtagtctt ttgcaatctt
      721 agcgatcgca acaccttttt ttagatcctc aacgatgttt ttataaacaa gacgacgttg
      781 gggatctttt gcattggcac tgtatagctt aggacgtcct ttataaacac cgttcgcttt
      841 tgcaatctta atgccttgtg cctgacgtcg tttagattcg gtacgttctt gttcagcgat
      901 catggccaaa atttgaatga tcaaatcttt aatgaattta tccaggagag gattgccaat
      961 tgcttcggcc atgatcggta gacttgtgat gatcagctgc acatttttct tcttcaagta
     1021 attgactgaa gcgatgatct cgtcgtaatt tcttccaagt cgatcaatag cttccacaat
     1081 aaaaatatct tgatctcgga cgaaatccag tgcttcttga aaaacaagtc gatgagtaat
     1141 cgttgcgcca gattgtttct caatgaaaat tctctctgca ccgaattttt tcatttcttc
     1201 aatctgccgt tgttcatttt gatcgatgga tgagacacgt atgtatgcaa ctctcaaaat
     1261 ttttacctac tttctgcaac tatttaaaaa acaccctaat catacacgag aaaaccagat
     1321 ttttcaatgt cgcggtcgtg tctagttagg gtatacctta tatagacaaa aactaatgat
     1381 ggaggaaata gtatgtgtca cttttctttt taaatggcaa tattttttgt aaataaatgt
     1441 caatcaagaa aatttaaacg attatgacat taaaaatata ttttatgaat tttctagtat
     1501 ataacttgat ttatctgtta agatttatta agaaaacgtt ttcttatttt tgatttcgag
     1561 gtgatagaca atgaaaaata agtttatttt atctttaggt attttaacta ttggaattag
     1621 tgtaactgga actacaattc ctacatttgc tgatagtaca aatgaaccaa ctactgtagt
     1681 agataataac caagaattaa ctcctttaga taggaacgga atagccattg gtaacccaac
     1741 taacgactta actgttactg aaaattattt aatagtttct ccattacttg ctcgagcagc
     1801 ttctaaaacg atacgagtga caggaacgat gatttttgat aaagttggtt ggccttctag
     1861 acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt tattggtact atggaaaatt
     1921 gtatttacaa agttataaat cagctggaaa caattggatt gcgacttatt caggaacctt
     1981 aactagaaag tagggatttt ttatgccaga atttgtgaac acaccaaatt tttatcaaga
     2041 tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa tcagctttac aagttcagcg
     2101 aactttacag gagacacaaa aaattcaaaa atcaatttct aatctagaag attctgaaga
     2161 aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa
     2221 gagtgtctag tcgacattct ttttttctaa ttgaaagtgt tcataaaatc acccatttct
     2281 ggacactttc tttttttgct ttatttttca aaaaaacaat tcatttcatg ttcataaacc
     2341 cataacaata tctatgttca gttaaactat atggataaag ttatgttaca ataaaattaa
     2401 tgaaggagtg atttttttga aaataggata cgcccgggta tctactggtt tacaaaattt
     2461 ggatttacaa gaagacagcc taaataaatt tggttgtgaa aaaattttta ctgatcatat
     2521 gagtggggca aaaagtaatc gaccaggatt agaaatggcc attgattttg ttcgctctgg
     2581 cgatacactt gtcgtttggc gcttagatcg tttaggtaga aatatggaag atttgatttc
     2641 aatcgtcaat cgactgaatg agcgtggtgt gagttttcat agtattcaag aaaatattac
     2701 gatggacaaa tcaagttcta caggacagct catgtttcat ttgtttgcgg catttgcaga
     2761 atttgagcgt aatttgatcc tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag
     2821 aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat tactgaagac
     2881 tttagtggat agcggtacac ctattaagac tattgcggaa cggtggaacg tctcaagaac
     2941 caccatttat cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa attctcatta
     3001 gtttttgtca tttaatgctg aaaatttttg ccatttatct agaaaagtga caatagtatg
     3061 agaatttttt gtaagtgtct actgataact tattctcaaa tgttgtaagg attttattca
     3121 tttgttaata tta
//
LOCUS       DACCIQ010000154         3056 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCIQ010000154 DACCIQ010000000
VERSION     DACCIQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.161"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..2878)
                     /gene="esp"
                     /locus_tag="IVM62_002715"
     CDS             complement(<1..2878)
                     /gene="esp"
                     /locus_tag="IVM62_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP6846622.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVK"
BASE COUNT          816 a          631 c          482 g         1127 t
ORIGIN      
        1 ctttcactgc aaagactgtt gttccacttt caatacttgt tccttctcct gcagtaaagg
       61 ttactttatg atagccagcc ggaatgttat cccctgggtt ttcaatgata tcatccaatt
      121 ttgttgcact tgatacaaat tctgtatcat ctgctgtaat tggttgagtt gcttcttctg
      181 gccatttcgc atctgtataa ccatcttttg ctttcaacac tggaagttta tcttctggca
      241 agcttacgcc gtctttcact gcaaagactg ttgttccact ttcaatactt gttccttctc
      301 ctgcggtgaa ggttactttg tggtagcctg caggaatgtt ttctcctgga ttttcaatga
      361 tatcatccag ttttgttgca cttgatacaa attctgtatc atctgctgta attggttgag
      421 ttgcttcttc tggccatttc gcatctgtat aaccatcttt tgctttcaac actggaagtt
      481 tatcttctgg caagcttacg ccgtctttca ctgcaaagac tgttgttcca ctttcaatac
      541 ttgttccttc ccctgcggtg aaggttactt tgtggtagcc tgcaggaatg ttttctcctg
      601 gattttcata aatatcttta cttacagtta ctgctaaatc ggtcgtgcct acagaaccat
      661 cttggtaaac aattttaata ggaattgttc cagtaatcgg ctccgattct cctacacctt
      721 tagctggagt gaatgtaaca ataccagtgt ttgcatcaac acttgcatta ccgaagtcct
      781 tattatagac atctgaaatt gaaaaggcat ctttagctaa aggaacatct ggtttatcaa
      841 atgtaggttt tccactttga actgtcgttt ctggagttgt tgttataggt acgtatgttg
      901 catcattttc catagcttga gaagctttta cagcaaactc tacatccacg tcgaaagttc
      961 gatttccgat cttaacagtt acaatacctt tcgtactatc agtaacgtta gaagtatcag
     1021 gctctgtttt ccatgaatac tctgcatctt caggtaagtt tttattattc cgaattgatt
     1081 ctttagcatc tggtttaaat ccaactacca cggtttgttt atcaaccgct tttggtgatt
     1141 ccttaataac ggttgaacct tcttctggtt tatcaaaacc tggagaaacg atttggattt
     1201 ttaattcatc tttcgcgatt aatttacttg aatctacacc cgtaaattca aattctacag
     1261 tatctgaaat tggagcccca tctttttcat ttggagcgat agttttttct gctaatacag
     1321 taccttcttt atcaacaagt tgggctttgt gacctgttcc cgctaactcg tggatgaata
     1381 ctttcccctt aactgttgtg tcaacatcat atacttcttc taattcaggt ttcccgatat
     1441 atggattcgt gtctccgctc tcttctttat cgtcagggtt tccatcacca tcaatatctt
     1501 gcaaataata ataacctgtt ccataagtgt tctggattaa ttttttattc gtatccgaca
     1561 accaagaatc aaagatgaaa tcttcaccaa atggtttatt ttcttcaact tgtgctgttg
     1621 ctttcatatc ttctaagatt tcatctaatg gcttagtcaa ttgtaaaaca tatcgaataa
     1681 cgactggacg tggacttgca ttagcaaaat cgataagttt agttaaatca ttattagtta
     1741 ttgaacctat gccataattt tcatgacctt cccgtcgctc gattgctaaa tcagctactt
     1801 ttgttccgga ttcaaacgaa acatcatatg ttactttgtc agcctgaacc atgtgcatct
     1861 gtatacccgc tacatatgga actaatcgtt catcaatttt gtaattagca taccaaggcg
     1921 tgccctttgc ggaagatgaa tatgagaaat tataattttt cgttaactta tgatctaaga
     1981 ttatagctcc attagcacct gtggatcctg aaccatcttt tgtttgagta ttaggtttct
     2041 catattttgc acttactgat ccatgtttaa actggtcagg attattttca gatacatcta
     2101 agctattata gaaatcatct gaatctgtta aaaagtagcc cgtactatca atacccggta
     2161 caattttatc atcgtcttga tgacttacca agtagattcg atacattaaa cgatcggtct
     2221 tgagattgtc tatgcctatc tctttaaaaa tttcatctag gctcttttca aacgtaatta
     2281 ccccattagg agcggtttgc gtatcggttg tttctgcccc agcaaataat ccatcacttg
     2341 cacgaatata attgaccttc caaatatttg taggtctacc cagcgaatca ttaattctta
     2401 caaaggtccg tctagaagat ccaattcctt tctttggatc gacctgaatt ttttctacat
     2461 atttagctaa tcgttcatct aattgaagac ggatttccca gtttttagtt gatgctgcaa
     2521 tattatgttt tccgtaaact tcaaatccaa ttgtattgcc ttttagtgtg tctccagtta
     2581 gttcttgagg actaaattgt aaatccgaaa agttataatt tttcgtataa ccaggattct
     2641 cctgaaaagt attccccgaa gctactttgt cacctacttg tacggagctt atcgctgttt
     2701 caagtgggct gcttggagaa atttcatctt gtgctttaac tagttcagtt gttcccaaga
     2761 caagaccaac accaatcaaa acagatgcaa ccccaacact taattttttg atactgtact
     2821 tcaatactcg tttctttgtt ttttctagaa atactctctt attattcttg ctaaccatta
     2881 ttcaaatcaa ctcccttaat aattactttc ctagtaagaa taaaagataa atctgatacg
     2941 tattattcac agaataggaa tcttactaac cttatgttaa ctaatttact tagagaattt
     3001 catgaacatt acaacaaaaa aaagtacatt tcttcatgtt tttttcggat atttgt
//
LOCUS       DACCIQ010000155         3042 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCIQ010000155 DACCIQ010000000
VERSION     DACCIQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.162"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..1272)
                     /locus_tag="IVM62_002716"
     CDS             complement(<1..1272)
                     /locus_tag="IVM62_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6846623.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNN"
     gene            complement(1291..1695)
                     /locus_tag="IVM62_002717"
     CDS             complement(1291..1695)
                     /locus_tag="IVM62_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP6846624.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1927..2184)
                     /locus_tag="IVM62_002718"
     CDS             complement(1927..2184)
                     /locus_tag="IVM62_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP6846625.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(2309..2641)
                     /locus_tag="IVM62_002719"
     CDS             complement(2309..2641)
                     /locus_tag="IVM62_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846626.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT          944 a          564 c          405 g         1129 t
ORIGIN      
        1 attattttgg atatcaacat tcttttgtct aaaatatcta tcatatttct caaaaagctg
       61 ctgcttatca gtgtctttct tccacgtttg ataaagatac tctataagtt cgttgatatt
      121 aaaaacttcg tctacttttt ccaaatcatc catttgaata tattttaatt cattctgtct
      181 tttcataacc ttcatgccgt tgaaattcac ccattgtggt aaatgataca tctgaatata
      241 aagttcttcc aaatcatcta agaaataaaa ggcattggca caggtcaatt gaatgatatt
      301 tttcaaattg ccaacatttt cactaaagcg atagtttaat aaaaaatcta ataattgttg
      361 agatatcagt attttctttg ctagtctttc ttcttctgat ttaaaaaata tcttgattaa
      421 ctgttttctt tcataaagag aacgactgtc taatgttgga agtgttagct ttacaggtat
      481 tcttctcaac aaagttgata atagtttatc ctcaggtggt tctgatgttg caaatataat
      541 ccagcaactt gatgtgtacc agttttcatt atcacccatc cgatgaaatt tcctcgtatc
      601 cataaactga aaaagtttct cctgacattc attgctcaat gcatgaattt catctaaaaa
      661 taagactcca ttattagcta attctgctaa accctttctt ccatcttcgg ctcctgtata
      721 tgcacctttt acatgcccaa aaaaattgga caagaaaagt tctggattat tggcatattc
      781 actacaatta actgaaatga aagatgcctt ctcttctata taattattgg ccaacaaatc
      841 cttatacaca taatttgcta aatagctttt tccagttcca gtctcgccat gaagtaaaat
      901 gggtaagcca tttggcggat agtagattgc tgctttaatt tgagcaatta catttcgcag
      961 acttccttga cttccgataa ccctttctat tccaacccta aaagagctat cataccattt
     1021 ttccgcttcc tctgaagagc taaaaatttg ttgatttgga actttctccg gtaaagaaaa
     1081 gaagattact ggacgagtat tgatttttcc aaattctcca tcttttacga attcattcaa
     1141 atattgagag acaatattac gactaacatt taatttttca gatattttct gtgctgtatt
     1201 gttcccccat ttatttttaa tctcagagtt ctctatgaca taattgacaa tgcgttcttt
     1261 taattcttcc atcacagcac ctcctcgaca ctattcgaat tttgccgaca gtatttttcc
     1321 ataaatatga cttgctaaaa aggctttgtt tagatcattc aattggaacg ttgcttgttt
     1381 tgcttgatcc gctaagacgt tgaaaagata ctctggcgaa atcgcatacc ccaaaatatc
     1441 aacgattgtt gcaatcagat cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     1501 catcgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     1561 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agccccaata aataattttt
     1621 atattctgac gcatccattt tactacggag attatctgcc gcactaaata acttctgatt
     1681 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     1741 tattttagca tactcagttt tagaaaaagc aaaaaaataa agaaacttat tcttgataac
     1801 aagctacgtg acgtatatcc ctaacacaaa aatagccata tcctttggtt tggatacggc
     1861 tatttgatat tactcattca attatcaaat cacagtaggt gtgaatctca tcgggatcgg
     1921 caatatctac ttctgattag gatcaaaggt gattttctca ataaaggtgt gatacaaagc
     1981 tttaacttgc ttcttctcac tctggataag aaagcaatcc aaactagtca atatctgttg
     2041 gacatctttg acttgaatcg gttccccttg ttgttgaaag atccctaaga tttcattctc
     2101 tcgaatttgt aactgtcttc gtttttcagt caattcattt aaacgatctt ttaacatcgg
     2161 aaacaattcg ggactatctt ccaaagcagc ttcctgtttt tgctctaata gttagatttt
     2221 gcgtaccatt ttcttctttt attttccttc gattgctgat tttaatatag tctaaaggaa
     2281 atttatattt attgtattct ttataacttc aagagaactt cgtttgagta ttactgtatt
     2341 tttttaattc cataagaaaa ttagttatta cctgccacct aatcagcaaa tctgaacctg
     2401 agtcaatata attaatatta ttttccatct ccaacattgt aagtactgtt ggtgttaata
     2461 tagggacttt gtacaattgt atttttttga ttttatcatc tgcacaaagg agatttttta
     2521 aatctaatcc ttcttttatt tcaaaagttg aattttttaa atctataaga gtaaaaccaa
     2581 ttccttcata ctgaatttct gaaaatcgaa tttttctccc agttgataga tcaaggggca
     2641 gaaaaccttt tattacattg tgagtgtcac catcatttac aatgtcatta ggaattttga
     2701 ttttagaatt ctgtggagca cctattggaa gataataatt cattataatt ttgtagtctt
     2761 catgaggaaa taaagtactt ttaggattgc taagatattc tcgtaccgaa tctaagaatg
     2821 gcttatccat tgaatcttca tccccattta aactaaaaaa cattcctaca attgcttttg
     2881 caactctaag tgaattcatt ttctttattg aatgtgttaa atcagactta ccattcttta
     2941 aacttgtatt gttataaagg aaatttttat tgtcttctgc aaatctaata tattcctcaa
     3001 catactcact accaaaaaaa ctattacatt tactacataa cg
//
LOCUS       DACCIQ010000156         3032 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCIQ010000156 DACCIQ010000000
VERSION     DACCIQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.163"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..493
                     /locus_tag="IVM62_002720"
     CDS             <1..493
                     /locus_tag="IVM62_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP6846627.1"
                     /translation="KQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDII
                     YITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINAN
                     LNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQ
                     IAY"
     gene            466..1143
                     /locus_tag="IVM62_002721"
     CDS             466..1143
                     /locus_tag="IVM62_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6846628.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1133..2275
                     /locus_tag="IVM62_002722"
     CDS             1133..2275
                     /locus_tag="IVM62_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP6846629.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2288..>3032
                     /locus_tag="IVM62_002723"
     CDS             2288..>3032
                     /locus_tag="IVM62_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6846630.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWE"
BASE COUNT         1130 a          388 c          582 g          932 t
ORIGIN      
        1 aaaacaaatt ttagctttga tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat
       61 tttacctaat agtgatgctg gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa
      121 aacggatatt atttatatta cgaatttaga gccggaggaa tacctgacta tattaaaaga
      181 gtgcaaattt atgatcggaa attcttcttc tgggataatt gaagctgcat cattcaaaat
      241 accagtaata aatttaggaa ccagacaaaa tggacgagaa caatctaaaa atattataaa
      301 tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat aaaatacaag atcctcagtt
      361 tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt ctagaagaat
      421 aacaaaaaca ttgaatcaaa tattgaatga tggggaagat attaagtgga ttcaaaaaca
      481 aatagcttat taaaatatac tagtgatcca tacattatta ttggggctgg cggacacgca
      541 aaagtcatta ttgaaacctt aagattagaa aataagttaa tatatggatt ttttgatgat
      601 aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt gaatagctgg
      661 gcggatagca aaaaaaaaat ctataaattt attgtagcta ttggtgactg tgtagtaaga
      721 aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca
      781 agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct
      841 gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt
      901 gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt
      961 ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag
     1021 aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca
     1081 cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc
     1141 tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc
     1201 ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat
     1261 tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat
     1321 acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt
     1381 tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga
     1441 ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc
     1501 tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc
     1561 tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc
     1621 agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg
     1681 gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc
     1741 tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc
     1801 accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg
     1861 tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt
     1921 caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga
     1981 tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga
     2041 tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg
     2101 gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa
     2161 tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaagtaatat
     2221 gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca agtaggtgtg
     2281 atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa gacttagact
     2341 cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg aggcgagacg
     2401 aaaggaagtt gaaaaggaat ttggaatacc aacttataat agtctaacag aggcaaaaaa
     2461 tttgtttgaa ataaattcag tttttgtttg tacatcccct ataacccatg aatttattat
     2521 aaaagaggct ttaaatttag attgtaatat ctttacggaa atcaatttat taaatgaata
     2581 ttatgatgaa ataatcgaag tagctaaaaa gaaagataga ttactttatc tatcttctac
     2641 atttttgaag agaaaagaaa ttcaatatat taagtctaaa gtgacgcata ataaaaaaat
     2701 ttcttatcaa taccatgtag gacagtattt acccgactgg catccttggg aagattatcg
     2761 caattttttt gtttcaaaca aaaaaactaa tgggtgtaga gaaatattcg ctattgaatt
     2821 accttggatt atagatacat ttgggaaagt taaatcgttt actgtaatga cccagaaatt
     2881 atccaatctt gatattgatt attcggacag tttcactctt attattgagc atgaaactgg
     2941 tatagttgga actttaagtg tagatgttgt ttcaagagta gctaaaagaa atttaactat
     3001 aattggtgaa aagattttaa tagaatggga ag
//
LOCUS       DACCIQ010000157         2820 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCIQ010000157 DACCIQ010000000
VERSION     DACCIQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.164"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..193
                     /locus_tag="IVM62_002724"
     CDS             <1..193
                     /locus_tag="IVM62_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAP6846631.1"
                     /translation="KRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPS
                     FSQPYNFILKAVTGLNIET"
     gene            305..1756
                     /locus_tag="IVM62_002725"
     CDS             305..1756
                     /locus_tag="IVM62_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6846632.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2105..2737
                     /locus_tag="IVM62_002726"
     CDS             2105..2737
                     /locus_tag="IVM62_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP6846633.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1065 a          403 c          538 g          814 t
ORIGIN      
        1 aaagagaata gtaggtgtac aactttgtag tcaagaagat ttatctggac tattatcaat
       61 gttttctctt gctatagaag aacaaattac attagacaaa ttgaaaatgt tagattattt
      121 atttcatcct agttttagcc aaccctataa ttttatttta aaggcagtaa caggattaaa
      181 cattgaaaca taatcgttac taaatgaaat caagaaatat actgtattac tcaattcatt
      241 gaaaatattt atcataattt ttaatagaaa acactattaa agagaaaaaa gagaggtaaa
      301 tcaaatgaaa aaatctttaa aacttgcatc agcagttata ttaatgtccc aactccttat
      361 agcaacacct ttccatgtgt tggcaagcga aaattcaaca tacaaaattg caaaagaaag
      421 aaccgagacg actgaaatga gtaattccga tgtaacagca gagagtagtg aacttttgac
      481 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcatcaag
      541 tgaaagtagt acgacttcaa atgccgagtc ttcaacaact tcatcaagtg aaagtagtac
      601 gacttcaaat gccgagtctt caacaacttc gtcaagtgaa agtagtacga tttcaagtac
      661 cgaatcttca acaacttcaa gtagtacaag tgaaataact tcttcgaaag tgacaaaacc
      721 aaaccatata gaatcttcaa aaaaggaagc gccaaaaacg gaaggacaaa caaaaacttt
      781 ttcgacacca cagcaaatag aatcaacaga atctttccaa tcacaaccaa tactatctgg
      841 taatgaagaa attcattttg acaaaaatga atctgtagaa aattttatta tgaaaattgg
      901 tgagtctgcg agaaaaattg gtcagaaata tgatttatat gcttctgtta tgattgcaca
      961 agcgatctta gaatctgcca gtggtcaaag ccagttagct caggcaccga attacaattt
     1021 atttgggata aaaggaactt ataatggaaa ctttgtcatt atggtaacaa acgaagattt
     1081 gggtaatgga acgctttata cgacccaatc aaaatttaga gtgtatgaaa actatgagga
     1141 aagctttgaa gattatgcca aattattaac taagggaatt agtggaaata aagattttta
     1201 tgcaggagca ttaaaagcta atagtaaaac atatcgagaa gcaactaaat tccttacagg
     1261 acggtatgca acagatacac aatactattt gaagctaaat gaattaatta aaacctacga
     1321 tttaaccaac tatgataaag aagttaaagg tccacaaatg agtaagactg gataccaagt
     1381 gcctattcat aattattcaa taaccagttt gtttggaaat cgtggaaatg aatttcaccg
     1441 aggacttgat atggcagcta cacagggtga gccgattcat gcagttaaag ctggtaaggt
     1501 tgttaaagca gagtttcact actcttgggg aaattacgtg gtgatacaac acgaagatgg
     1561 atcaaccgcg ttatatgctc accagcaaca atacctagta aaaactggag ataatgtttt
     1621 gcaaggacaa ataattggct atgtaggaag tactgggaat agtactggcc cacaccttca
     1681 tttggaaatt tgcaaagata gtagtctttc tcagagcatg ttagttgatc cgaaaacctt
     1741 attattcgga gactaaaaaa ggtaatcaat ctgacaaagt aacaagaaaa tggttggatt
     1801 cttagacctt tgatcaggat attgagactg gaattgatat ctcgattacg atgggtttga
     1861 caaataaaac aagtccatta tcgaatatcg agtgtattat tgccgtcttt atgctcatat
     1921 tttgagcagg tttcactaga tgaaaacaat ttatctatct tgaggatttc gtgtacgtat
     1981 cgttcagtct tgtattgtaa attagtcata aaatcagact aagaaaaatt ggaaatattt
     2041 ttttctgatt tattaaaagt taattgaaat tatatggtac ttctttcttg ggagggtagg
     2101 tgcagtgaag gaaagatatg gtcaccttac tgttgttgag gagattactc ataatggata
     2161 taaaaaatat cgttgcctat gtgactgtgg gaatgaaacg attgtatatc gatcaaactt
     2221 actatcaggt agaaccaaaa gttgtggatg tggaaattat aaaaataggc ataaatataa
     2281 aaatttgaca aataaaactt ttggtaagtt agttgcaatc aagccaacta aactgcgaag
     2341 tgaaggaact gttgtttggg aatgtcaatg tctgtgtggg agaaaaaagt tagtatcggc
     2401 aaagcgactt ctaaaaggtg atgtaaaaag ctgtggatgt ttgaaatcaa aaaagcatca
     2461 cattgaaaat aaaaaattcg gattacttac tgcaaagtat cctttggaca ataactgttc
     2521 atggaaaaca aaatgggttt gtcattgtga gtgtggcagt atgtgtgaga taagttatag
     2581 taacttattt tttggacata ctcaaagttg tggatgttta aaaaggcaag aaacaaggac
     2641 atgtgttgaa gggacagtaa tagagtgtct aacgtcaaaa ttaccgaaaa ataatacaag
     2701 tggtgttaag ggagtttatc gttctaaaga gaaatagatt gcgtatataa ctttaaaaaa
     2761 aaagaggtac tatttaggat cgtatgattc tattaagagt gcagctcaag ctagaaaaga
//
LOCUS       DACCIQ010000158         2772 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCIQ010000158 DACCIQ010000000
VERSION     DACCIQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.165"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(763..1266)
                     /locus_tag="IVM62_002727"
     CDS             complement(763..1266)
                     /locus_tag="IVM62_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6846634.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1625..2650
                     /locus_tag="IVM62_002728"
     CDS             1625..2650
                     /locus_tag="IVM62_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6846635.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          910 a          417 c          517 g          928 t
ORIGIN      
        1 agcagtatga ttctgttgta aacgtgtttt tatttgtgga aaatttaatt tttagaaaag
       61 tgtatcgaaa aatagatagt tagtttttcc acagtggata acttcgtaag agataaaaat
      121 aaaaaattag tcaaagaagg gcgtatagat ttcttttgat aggagatata caacagaaat
      181 gaataaatat taacgtgttt tcaaacagta taaaacggtg tggtttttgg aataaaaaaa
      241 taacatatct cattttacac ttttgttatt ttatacttaa aaaataacga cacagaaaat
      301 accgtgtctc agatttctct actacttcta aaaactactg tttttttcct ttgaaatgcc
      361 ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg agcctaaata
      421 aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa
      481 ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga
      541 ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata
      601 aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga
      661 ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac
      721 acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt
      781 tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat
      841 ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa
      901 acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc
      961 gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt
     1021 agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat
     1081 caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg
     1141 gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt
     1201 gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata
     1261 ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa
     1321 aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg
     1381 ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc
     1441 ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt
     1501 actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga
     1561 caaacgggat tggcatttca tcttggttca gctttttttc caattactag gaaggaggga
     1621 tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat
     1681 gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt
     1741 gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat
     1801 gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt
     1861 ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt
     1921 ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc
     1981 gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc
     2041 actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact
     2101 ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga
     2161 gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt
     2221 cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt
     2281 gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat
     2341 gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat
     2401 ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt
     2461 tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata
     2521 cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt
     2581 aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa
     2641 attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa
     2701 cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa
     2761 tgtggaaagg aa
//
LOCUS       DACCIQ010000159         2725 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCIQ010000159 DACCIQ010000000
VERSION     DACCIQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.166"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..541)
                     /locus_tag="IVM62_002729"
     CDS             complement(<1..541)
                     /locus_tag="IVM62_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846636.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQ"
     gene            complement(1255..1545)
                     /locus_tag="IVM62_002730"
     CDS             complement(1255..1545)
                     /locus_tag="IVM62_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846637.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1753..2445)
                     /locus_tag="IVM62_002731"
     CDS             complement(1753..2445)
                     /locus_tag="IVM62_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6846638.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          818 a          500 c          381 g         1026 t
ORIGIN      
        1 cttgttcttg attagttaaa aatatcaata aaggaatact tacccaatat atttttctat
       61 ttatttcttc tcctcttaag agttgaaaaa acggataaaa agttagtaaa ctaaatgcta
      121 ctggccactg atagttcaag gtggttgctc tccaaccggt ttcccccatt actgtcaatg
      181 gtattaaaca gtatatagag caaaccaata ataaattttt aacatttatt ttctcaaaaa
      241 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
      301 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      361 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      421 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      481 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      541 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      601 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      661 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      721 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      781 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      841 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      901 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      961 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
     1021 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
     1081 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
     1141 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
     1201 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1261 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1321 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1381 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1441 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1501 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1561 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1621 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1681 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1741 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1801 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1861 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1921 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1981 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     2041 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     2101 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     2161 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     2221 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa gcgcttgctc
     2281 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2341 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2401 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2461 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2521 attacatgtg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2581 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2641 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2701 attactgtat ttattacaaa aaacg
//
LOCUS       DACCIQ010000160         2686 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCIQ010000160 DACCIQ010000000
VERSION     DACCIQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.167"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            314..544
                     /locus_tag="IVM62_002732"
     CDS             314..544
                     /locus_tag="IVM62_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP6846639.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(767..865)
                     /locus_tag="IVM62_002733"
                     /pseudo
     CDS             complement(767..865)
                     /locus_tag="IVM62_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1130..1585)
                     /gene="tnpA"
                     /locus_tag="IVM62_002734"
     CDS             complement(1130..1585)
                     /gene="tnpA"
                     /locus_tag="IVM62_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6846640.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1828..2686
                     /locus_tag="IVM62_002735"
                     /pseudo
     CDS             1828..2686
                     /locus_tag="IVM62_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          865 a          488 c          477 g          856 t
ORIGIN      
        1 ggttatgaac aaaacaataa tattgattca gttgaattaa gagaaaaagt tttgcgctct
       61 attattgata aaagtattga gtgaactacc cccacttagc taaacctaac ggtttttaac
      121 gattgaagta ggcaattctt gggatgagag catacttgat agcttatttt ttactaatag
      181 cggtggctct tattgaccaa atatccctgt agttcctacg gttcttgatt ttatctaaag
      241 atatttttat cgtggaagct attgatcaac ttgaaagaaa aacgtgtagc tcattatcac
      301 tagtttatcg agtatggatg aagcaatgga tcatcctctc ttaaataaat ttattaagga
      361 tctgatcctt caaatttttt ccatgatcga tgagcaggaa cctactgaat ctaaacgaca
      421 tcgggcacaa ggaattatta agattgctaa gcgaatgttt tctataaagg gtgttctaag
      481 ctatacagtg ttaattcaaa agattcccaa tcatcttgtt tataagaaca acgttgaaga
      541 ttaaaaaagt gtttcaatgc taaaattgca aaatacttca atgttatgag acaaactgtc
      601 tataagaatc aatttccacc catttcatca cttgttaaaa gtttatgatc cttttgattt
      661 ttatattaat ttatctgtat ttaatacaga ttttatttat tagttcatct gcctattgta
      721 ttttattttg ttgccttacc atctgaagta aatatactct taattgtcaa attaagttag
      781 acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga taatccagtg
      841 attttctagg aagattattt ctgcgccagc attttctaat gtttgaattt gtgttttgta
      901 atcttggttg cttgtgctaa ttcttgaata gccgtaaatt gccaaaattt catcaccctt
      961 tctggacata agtttctgac accagttaaa atcaagtcta tcagtaaccc atacacccgt
     1021 gaggacgggt gtatttttaa tccccctaga aggggtcgac actcgctgcc ccttaaaagg
     1081 ggatttttaa aagaccgcca actgctcatc ttcactcact tacttctctt tattttttaa
     1141 atggttctac gtactctctt ttacttataa tatatcgaat gcgatcatca gcttcttgtt
     1201 cacggatgta tttttcattt gtcttttgat tcaatccgac tgtactcaca tagtatctct
     1261 tcgaccaaaa ggatttgttt ccgtatttat atttgagatt tgcgtgctgc tcgtggatta
     1321 gtaccgcact acgacctttc agatacccca taaaactgga tatacatatt tttggtggta
     1381 ctcgaacaag catgtgaata tgatctagca ttgcatgtgc ttctatgatt tccacatctt
     1441 tcatttcgca caacttccta agtatttttc caagatcttt tcgtaattgc ccatagatta
     1501 cttttcgttg atattttggt gtaaaaataa tatgatactt acaattccaa cgagtatatg
     1561 ctaaactttt atcgtcattt gacatgaaaa aactcctttc gatctgatga gcggttggta
     1621 gccactcact agtatatcga aaggagtttt tatcgctaaa gatacttctt ccacgcgtag
     1681 aaacgcgcgg ttttcttgtt tcgcacgctt tacgcacaaa actgactgaa gtcattaaga
     1741 aaataaaagg gtctaaaact tttaagtttt gtaccgaccc ccaaaagtta gaccaaaaaa
     1801 tctaactttt ggaggtcggt attttttatg gctaagtata cttttgaact taaattaaag
     1861 atcgttcatg attacttgga tggtaaaggc ggaagtgatt atctggcaaa gaaatattcc
     1921 attaaggctc catcacaagt gaagcgctgg atcaatgcct atcaagaatt tggtgaagag
     1981 ggtctagtaa gaaaacgaca aaagaaaaaa tattctgttc aatttaagct ggatgcgata
     2041 gagttatatc taacaagtga gctgtcctat cgtgaagttg ccaacacact taacatgaac
     2101 aaccctaatt tgatcgctag ttggatgcgt caattccgtg agggtgggat cgatggactc
     2161 tcaaaaacga aaggatgtcc acctatcttg tctaagaaaa atgaacccaa aaataagaaa
     2221 aaatctacaa ctaaagcaac gtctaaagaa cgtgaacgca tcgaggagtt ggagaaacga
     2281 gtccgttccc ttcaaattga aaatgccttt ttaaaagaat tgaggaagct gcgaaaacag
     2341 gaagcccaac aacgacgaat gaagcaatcg cacgaatcat cgcaagcctc cgaggaccgt
     2401 ttaaattagt tgagcttcta gaaactttaa agttccctaa ggcaatcttt atgtactggc
     2461 aaaaacgatt ggaccgtaaa aatccagatc aagagattga agcggaaatg cttaaagttc
     2521 gggagaagca caaggattat ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt
     2581 tctctatcaa taaaaagaaa gttcagcgcc tgatcaagaa gctaggaata aaagtaacga
     2641 catacacacg taaatcacgg cgctacaatt cttatcgagg acaagt
//
LOCUS       DACCIQ010000161         2643 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCIQ010000161 DACCIQ010000000
VERSION     DACCIQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.168"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            12..1019
                     /locus_tag="IVM62_002736"
     CDS             12..1019
                     /locus_tag="IVM62_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6846641.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1031..2035
                     /gene="neuB"
                     /locus_tag="IVM62_002737"
     CDS             1031..2035
                     /gene="neuB"
                     /locus_tag="IVM62_002737"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP6846642.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2032..>2643
                     /gene="neuC"
                     /locus_tag="IVM62_002738"
     CDS             2032..>2643
                     /gene="neuC"
                     /locus_tag="IVM62_002738"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP6846643.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYV"
BASE COUNT          977 a          337 c          523 g          806 t
ORIGIN      
        1 aggagatata agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg
       61 gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta
      121 gaggtgaaga aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat
      181 ttttaattgg tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata
      241 ttatttttca tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag
      301 ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg
      361 taaaacattt tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg
      421 cgacaaagtt attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag
      481 aaacaatatt ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc
      541 ctctttttag taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga
      601 cacgatttat gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt
      661 caaaatttgg agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc
      721 aatcattaat aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag
      781 ttgtaggtat caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg
      841 aacgctcata tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg
      901 aactgtacaa agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat
      961 taatgagtgg acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag
     1021 gagttttatg atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg
     1081 aagtctagat ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt
     1141 taaatttcaa acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata
     1201 tcagaaggat aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt
     1261 atctcaatct gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt
     1321 gtctactcct tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt
     1381 taaaattggc tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg
     1441 gttgccagtt ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga
     1501 ttgtttcata gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc
     1561 tgaagatatt aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg
     1621 gtactccgat cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa
     1681 ggtaattgag aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc
     1741 tttagatcct caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt
     1801 aggtgatggt gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa
     1861 aagcctagtg gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac
     1921 aataaaacgt cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa
     1981 agttaaaaga ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa
     2041 atacttatag taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat
     2101 ttactacaag ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa
     2161 tatggattta ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa
     2221 agtttgtttt tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat
     2281 ggtttcacaa atatatttga acaaactagc cctgattttg tattattact aggggataga
     2341 ggagaaatgt tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt
     2401 cacggaggcg aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata
     2461 gcatctgttc attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa
     2521 gattcttgga gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga
     2581 ttacctagtt ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac
     2641 gtt
//
LOCUS       DACCIQ010000162         2631 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCIQ010000162 DACCIQ010000000
VERSION     DACCIQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.169"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(61..2424)
                     /locus_tag="IVM62_002739"
     CDS             complement(61..2424)
                     /locus_tag="IVM62_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP6846644.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          895 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt t
//
LOCUS       DACCIQ010000163         2602 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCIQ010000163 DACCIQ010000000
VERSION     DACCIQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.170"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1350
                     /locus_tag="IVM62_002740"
     CDS             <1..1350
                     /locus_tag="IVM62_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6846645.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2602
                     /locus_tag="IVM62_002741"
     CDS             1484..>2602
                     /locus_tag="IVM62_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6846646.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSR"
BASE COUNT         1019 a          361 c          516 g          706 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gt
//
LOCUS       DACCIQ010000164         2511 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCIQ010000164 DACCIQ010000000
VERSION     DACCIQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.171"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..607
                     /locus_tag="IVM62_002742"
     CDS             <1..607
                     /locus_tag="IVM62_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6846647.1"
                     /translation="FILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYCWK
                     VFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLLPI
                     IWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLSRV
                     VSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            680..1609
                     /locus_tag="IVM62_002743"
     CDS             680..1609
                     /locus_tag="IVM62_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6846648.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1767..2504
                     /locus_tag="IVM62_002744"
     CDS             1767..2504
                     /locus_tag="IVM62_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP6846649.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
BASE COUNT          870 a          358 c          469 g          814 t
ORIGIN      
        1 ttttatacta cctacacttt atagcctttt atgtgtaatg gcaccatgta ttatttatat
       61 ggtatttttt tgcaaaaaaa ataccctgaa acacaatatc ctgattttga tttttatgct
      121 gtactgttgg aaagtttttg aattgactgg tacaggtgga ctaacagata ttttttatag
      181 acctgataca ggtaggaaat tttctctagt acagggcagt gttaatcttg taccattcag
      241 tggactaaac attagcttta tattaaatat agtaatgtgt gtgcccttag gattcttact
      301 accaatcata tggaaaagtt ataggaaaat agttcctgtt ttcttggctg gggctctatt
      361 ttcactaatt attgaagtct cacaaattat aactacaaga gcaacagata ttgatgattt
      421 aatagcaaat acctttggtg cggttatagg ctttatactg tgggctgttt taagtagagt
      481 ggtgtccttg aaggacaagt catctacagt agattattta gaaccttata tttatatatt
      541 gattggtttt accggaaaat tcttactgta ttttcctttc tggtttgctt ttgaagtatt
      601 atcctgataa ttccatagaa aataatgagt ttgagttgag aaagaatatt cttgtacaca
      661 actctggaaa ggaggaagta tgaaaagatt cttaaaatgg gggcttgttc ttttagcgtc
      721 tttgattgtg attctaatgt gtacagcggt tgttatttct tatgtgaatc ggccgtatgc
      781 aagagctgtt tatgatagga ttaaacgcat tcttgacaaa gatttttatg accatctata
      841 tctaggttct tcatttgaaa taataaacct tgatacaatg gatattcata gctatatgaa
      901 aaaagaccaa gaagaattac ccgcctcatt gagcaaatta tttacgattg attatgcgct
      961 tagtgtccta agtttagatg aacagattac aattagtcct actgcacttg acatgcagaa
     1021 agctgactca tctactgcac atcttcaaga aggagatatt ttatctgttt atgacattat
     1081 ctgtgctatg gtcttaccct caggaaatga tgcagcttat agtttggcaa tttatacagg
     1141 tggaaaactt gatcctgatg caaagaatga tgaagaacgc tatcaaagct tcatgaaaaa
     1201 cttagatcgt tatctaagtg agcaaggcta taaacatact cattttactg acgctgctgg
     1261 ctatgactcc aatgctacaa catcagcgga agatttgaca aaagtagctg caaagctact
     1321 aaagaatgat aaatttgcag aaatagttgc caaggaaact tatagagcaa aatcaattaa
     1381 gggaaatgtt tataactgga atactacgaa tcaatttatg caacaagata atagatactt
     1441 tactgaaggt gtaaaaggtg taaaaactgg atcgtttgat gggagataca atctatgtgt
     1501 gtattataca tctagttctg ataaccacta tttacttgta agttttggtt cagagagtga
     1561 tagtaggcgt tatgtagatg ttcaaaatct attgcataat ctgccttgaa atttcgtgcc
     1621 tttaagcgcg tatctaaagt ttctttgaaa agtttgagaa tgaagaaagt gagcagtcga
     1681 aaaacggttg ctcacttttt tgccctaaaa tcgaaaaaat ataaaaaagt cctttacaac
     1741 acgtctcagg aaaaactgct aagtcaattc taatatcttg tggagcaaga cttgcacctt
     1801 ttgatataag agagcttaga gaactaatga gcgaagatga actagaactc gataaaattg
     1861 gagatagaaa aactgccttg tttgtaataa tatcagatac agatgataca tttaattttg
     1921 tagtttcaat aatgtattct caattattta atctactatg tgataaggca gatgatgtgt
     1981 atggtggaag acttccagtt catgttaggt gtctacttga tgagtttgca aatatcggtt
     2041 tgattcctaa atttgaaaaa ctaatagcga caattcgttc aagagaaata tcggcaagta
     2101 taatactgca agcacaatct caattaaaag caatttataa agaccatgca gatacaatag
     2161 ttggtaactg tgactctaca ctttttctag gaggaaaaga aaaaacaaca gtaaaagaat
     2221 tatcagaaac cttaggaaaa gaaaccatag acctatataa cacatcagaa acaagatcaa
     2281 atcaaaaatc ttttggtcta aattatcaaa agactggcaa ggaacttatg agtcaagatg
     2341 aaataactgt aatggatggt ggtaagtgta tttaccagct aagaggagca agaccttttc
     2401 tatctgataa atttgatatt acaaagcata agaattacaa attgttggaa gattatgata
     2461 agaaaaactt gtttgatgta gaagagtatt taaaacaaag atagcgtaaa a
//
LOCUS       DACCIQ010000165         2448 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCIQ010000165 DACCIQ010000000
VERSION     DACCIQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.172"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            21..980
                     /locus_tag="IVM62_002745"
     CDS             21..980
                     /locus_tag="IVM62_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6846650.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1113..1319
                     /locus_tag="IVM62_002746"
     CDS             1113..1319
                     /locus_tag="IVM62_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6846651.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1330..2349
                     /locus_tag="IVM62_002747"
     CDS             1330..2349
                     /locus_tag="IVM62_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6846652.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          846 a          412 c          488 g          702 t
ORIGIN      
        1 attataagga gtgagtacaa atgacctata cccatcttac aacggatgaa cttgtaataa
       61 tagagtctta tttcaaaatg aatcaatctg ttgctaaaac tgcccattgc ttgaatcgtt
      121 caagacaaac gatccataaa gtatatctgt tcttcaagca aggaaaatca gccttagaat
      181 attatcaaca gtataagaaa aacaaatcaa actgtggtag acgtccgctt gttttacccg
      241 aggaacaatc agaatatatt caaagaaagg ttgttcaagg atggacaccc gatgtgattg
      301 ttggtcgtgc agcgtttcct attagttgtt ctgctcgtac catttatcgt atgttcaaaa
      361 aggggctatt tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc
      421 aagaaagacg tggaaaacaa actttccgcc gctctattca tgaacgtgaa aaggattata
      481 gccaattctc aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata
      541 aaagtgctgt aattacctta gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt
      601 gtggtagaca agcgattgat attgaaaaaa aattaaatca atggtttgaa tctgtaccga
      661 aaaacctatt caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga
      721 tcagtaatgt caatgatatt gccatttatt tcgctgatcc aggaacgccg tctcaaagag
      781 gcctaaacga gaattctaac ggattgttac gtagagatgg tttattgaaa tctatggatt
      841 tcaattcagt agatgaattt tttattcaat ctgtcgcatc taaacgaaat aatattccta
      901 gaaaatcact ggattatcga acacctttgg aagtattttt gagttacgta agtattgatg
      961 atctgtctaa cttaatttga caattaagaa agataaaatt gaacaactgg acgggcaaat
     1021 tgatgccttg ctttcagaaa gagaaatatt gatagaagaa aacaagcgat tgaaaaagaa
     1081 agtagcccaa ccaatgaagg gaggtggata gtatgaacaa tagaaccttt gatattttga
     1141 aatggatcgc tatagtagtt atccccgctt tagcgacttt tgtcggtctt gtcggaaaag
     1201 gtgttaactg gcggtacaca gatataacgg tgatgattat tacaggtttt ggagcatttc
     1261 tagggagtat tttaggtgta tcaaatcgaa cgtataagaa aatttcatca gataaatagg
     1321 aggatagata tgaaaaagaa aattactatt actgcgatga gcctgttaac ggctcttttt
     1381 ttattgccaa ttaacggatt tgcctatact atcaacaatg aatttaattt gggcgcaaat
     1441 gaaggtagct cacaagtagc aaataatcag tatattttac tgcatgaaac ggctaatgaa
     1501 acagcaacag gacgcaatga agcgcagtat atgcaacgtt catggactag cgcttatact
     1561 gcttatattg tgggagacgg cggaattgtt tatcaagtcg gtcaacctgg ttatgtacag
     1621 tacggtgctg gttcgtatgc taatgccaac agtcctgtgc agattgagtt acaacacaca
     1681 catgataaag caacgtttga gaaaaactac aaggcatacg ttgaattggc tagagattca
     1741 gcaatgaaat atggtattcc attaacgttg gacactcctt ataaccaacc gggaatcaaa
     1801 tcgcatttat gggtaacaca aaacatctgg ggcgatcata cagatcctta cggttatctt
     1861 tctgaaatgg gcgtaagtaa agaaaaatta gcatatgatt tagctcatgg atttaccgat
     1921 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
     1981 aatcctacac tgacagatgg aacgaattac gcccacattg atcagtttgg agaaatcgaa
     2041 aatgcaaatt tgcatgtagc tggatggcac attgctaact ataaatacga gtatatcttc
     2101 attatggatt acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
     2161 tcagatgtaa atcaagctta taatacttct ggaaatgttg gctatcatgt atcttttaac
     2221 atgcgtaact tccctaataa gaaagtctat gtcatgatgc gggcaacgaa tgatccagag
     2281 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
     2341 aaacgataaa aatagctcct cgttgaggag cattacataa ctatattgac aactataaaa
     2401 atcattcgat aaaatagtga tgttatcgca tatcttcact atcaccca
//
LOCUS       DACCIQ010000166         2445 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCIQ010000166 DACCIQ010000000
VERSION     DACCIQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.173"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(886..1404)
                     /locus_tag="IVM62_002748"
     CDS             complement(886..1404)
                     /locus_tag="IVM62_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP6846653.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          792 a          448 c          354 g          851 t
ORIGIN      
        1 agttatttgt tcttttatta aatacaccta caacttataa attgatggta tttagataac
       61 acgaaagaat aaaaataaca gctgatagta aagtttgttg acgattgcta ctcaatcgaa
      121 agtgagttag tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga ccttttacat
      181 cttgcagaaa tcgttactgg gattaaacta agcaataaaa actaatgttc ccctcattct
      241 ttgaacttct actatttgat ttatttattc tgataaatga cgatttcacc ttcaggatct
      301 tcaaatcccg tatagaattt atttaagatc tcttcagtat cagtaatagg ataggcgata
      361 ggccacatac cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt
      421 tagcatatta acaaaggcta tctccaaatt ataaaccata agcgttgaat cgttcttaac
      481 aatttcaatt cttcctaacc caaagtcaga agatataatt accggacttg ccttaccctt
      541 catgtgggtt gttgagattc catatttaaa gggaattgtc ggtactatcg gcggtctgaa
      601 aaacatccca aaaataaaaa aatctttccc ctgctagtct cctaagtccc tttgtagcgg
      661 atatggcacc ataatctcat tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa
      721 aattgataca atctaacgat gcaccctcta cggcattcta ggggagtgca cttgcattca
      781 cccaataaaa accggcagtt agcaaagaac ttatgtagtc gtatgttctt actgttaaca
      841 tacaagaggt ctcctttcca caattgctta gcagtaaacg ggaatttaca aagctaatct
      901 cttccatttt cttatctttg ttctaaaagt cccaaatgga gcaactgtat ttacatgaat
      961 aaatttatat acttcccata cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg
     1021 tgcagtgaat agatcttcat cactcattga atcaatcatc atgttaatat catcaatatt
     1081 acgattgaga atttcctcta attctttcaa tggtagatgt gcataggtat ttgtaaacca
     1141 cttatataac tctcctaact gattccattt aaattgatca gaaggcgttt taacagataa
     1201 tccatttctt tcatcatttt cccattttag aattaaattc gtccaaccaa cttgatatgc
     1261 taaattttca gcaggtgttc tatccacttc ctcaactctt ttatccttca attcttcagg
     1321 aatagtttca aattctaaaa tatatttagt ataagtccta tttatttctt tttttaattc
     1381 ttctttattt ttatatgttc tcattatatt acctccataa attcctaatt gtctccttta
     1441 attcatctat aatttcatcc acgatatttt aatccgttct acaatgcata tctgatatgc
     1501 attgactgtc taactcatat gtgaatgatt tataaaatat cccaacgtct catctaaaga
     1561 aatgttgatc gtcagcaagg agctaaaaaa tacgaatata tttcttttgt agtaaaatcg
     1621 gactggtttg catatcattt tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta
     1681 ctaataaaaa ctaccacttt aagcgttaag aactgttaag tttagttaag tgaggatagt
     1741 tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga
     1801 ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt
     1861 gacgatgaca cgttccttac ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa
     1921 ctcgaagaaa atttaattct aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact
     1981 attaaaggtc aagatttacc tgaagatcat cctttttagg aaattgaagc tttttatgtt
     2041 gcacaatgca tttataatac tacaatgatg ttttcaccag atattcttct agggtgcggt
     2101 gtaatgaaac agatacactt gcaaaagaaa attcaccgcg aatttactaa aattatgaat
     2161 aattatgtta atcttccgaa atttttttcc aaaattaagg gatcaagctg gtgttataga
     2221 ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa ccgatactct
     2281 gttaagttgg ttgacacggt gtcgattttt atgactttct tattctgaaa cttcactaac
     2341 ggacagtacc taaaaagtct atagtatgga cgtttacgag tactatttta tcaaaaatgc
     2401 tgctcatttc aacttatatg ttttagtgaa ctacccccac ttagc
//
LOCUS       DACCIQ010000167         2400 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCIQ010000167 DACCIQ010000000
VERSION     DACCIQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.174"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            207..2069
                     /locus_tag="IVM62_002749"
     CDS             207..2069
                     /locus_tag="IVM62_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846654.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IVM62_002750"
     CDS             2069..>2400
                     /locus_tag="IVM62_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846655.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACCIQ010000168         2394 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCIQ010000168 DACCIQ010000000
VERSION     DACCIQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.175"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..533
                     /locus_tag="IVM62_002751"
     CDS             <1..533
                     /locus_tag="IVM62_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6846656.1"
                     /translation="NGQSYADNFTIQGLLEYASRDSGYDGVGDLNRITGISRVVDEIF
                     NNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFF
                     IIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVK
                     RKEHYYSEKNTKGEIL"
     gene            530..1249
                     /locus_tag="IVM62_002752"
     CDS             530..1249
                     /locus_tag="IVM62_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6846657.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1264..2349
                     /locus_tag="IVM62_002753"
     CDS             1264..2349
                     /locus_tag="IVM62_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6846658.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          860 a          258 c          480 g          796 t
ORIGIN      
        1 ataatggaca aagctatgct gataatttca caattcaagg tcttttagaa tatgcgtcaa
       61 gagattctgg atatgatggt gttggtgatt taaatcgaat aactggtatc tctagagtag
      121 tagatgaaat atttaataat aattttagat tagtcctaat gggagtaggc attggcaatg
      181 cagagtttac caattttttt gtcagtgagt tttataaatc gtttggtaat cttcattatc
      241 agtattttca tcatatttgg ttatttatag aagttggaat attaggagtt tcgatattcc
      301 tattttttat aataaagaca tttttcaatg caaaaagatc ttttatgaag gataaagatg
      361 caagtcttgt taaagttatt gcaattatgt cggtattctt ttttgtgtat aacacaacac
      421 ttaggggtga aacaacgggg ccactgattt atatgatttt agccatgcct gaagttgtga
      481 aaaggaaaga gcattattat agtgaaaaaa atacaaaagg agagatatta tgattcctaa
      541 gaagatacat tactgttggt tcgggggaaa tccacttccg aaaaaagata gaaagtgcat
      601 agaaagctgg aaaaagtatt gccctgatta tgagattatt gaatggaatg aatcaaatta
      661 tgatttaaat aagaaccgtt atatggccga tgcatataaa gaacgtaaat ggggttttgt
      721 tccagattat gcaagatttg atattattta taatcatggt ggattttatt tagatacaga
      781 tgttgagcta attaagtcgt tagatatact tcgtgaaaat catgcctata tgggatttga
      841 aggggaaatc tgggttaatg gcggtattgg ttttggtgca gaagaaggac attctgttat
      901 taaaggttta agggatattt acgattcatt aagtttttat caaaatgatg gtacattgaa
      961 cttgaaaccg agtccgtatt atattacaga ttatcttgaa tctattggat tggttcgcga
     1021 taacagcgaa caaataataa atggcctgca tatctatcct acggattatt ttgctgccaa
     1081 ggattatgat actggaaaaa tttataaaac tagtaataca ataagcattc atcaatataa
     1141 tgcatcttgg aaaacaccta aacaaaaatt tgtttataga ttaaaatcaa caattggaca
     1201 aaaaaaattt aacaggttac ttgcactgaa aaaattttta cacttataaa ggagaaaagt
     1261 actttgaaga aaatttgttt cattagtggt gtaatttctc gttctggggg aactgaaaga
     1321 gtagggagta ttattgccaa tagcttgtct gatttaggtt atgatgtcac tattttaagc
     1381 ttttggaata gtggagagcc ttattttggg ttagataaga cgattaaggt tgattatatg
     1441 ttaaatccta aaacggaggg aaaattatat agaacttatt tatatccggt ttggaaattg
     1501 cacaagtata tatgcaaaaa tgaatttgat atagtaattg atattgatac tttgttgtct
     1561 ttgtatacta caagggcaat taaaggaaca aagtgcaaat tgatttcttg ggaacatttt
     1621 aattactgga caatggaaat gctgaaagaa aagaagagat tcaaggctaa aaaattgata
     1681 aaaaaatatg caaagaggct tgtggttctc acagaagaag atcgtcaaaa acatattgat
     1741 gaatatagtt tatcagaaga ttttgttata actatgccaa atccgtgtat atctaatatc
     1801 gagtacgaat atcattatga taaaaagaga tttcttgcag tagggcgatt agtggaagag
     1861 aaaaattttg ctgacatgat aagtgcttgg aaaattgtag aaaaaaaagt agatgattgg
     1921 gatcttatca ttgtaggaaa aggtgaatta gaaaaagatc ttcgccagca gataaaagat
     1981 ttaaatctgc aaagagtaac tatagcaggg cattcagacc atgtggagga atattataaa
     2041 tctgcatctt gttatgtgct tagttctgtt tatgaaggat ttcctatggt tatattagaa
     2101 gcacagtctt atggattacc agttatttct tatgattgta aaactggtcc gagagatttg
     2161 gtacatcata attttaatgg gatgttggtt gaagataaga atatagacca attagctaag
     2221 agcatgataa tgtttactaa gaatactgat ttagcaatga gaatgtcact gaacgcatat
     2281 aataatgtac aaaaatttaa tttgaaagaa attacaaaac aatgggttgc tttgattgaa
     2341 aatgtatagt tttttgtggt tgtccaaaca tgaaaattag cgtaattaat caaa
//
LOCUS       DACCIQ010000169         2378 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCIQ010000169 DACCIQ010000000
VERSION     DACCIQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.176"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..430
                     /locus_tag="IVM62_002754"
     CDS             <1..430
                     /locus_tag="IVM62_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP6846659.1"
                     /translation="HHRAIYTNEDALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFT
                     TKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEK
                     LVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            467..1477
                     /locus_tag="IVM62_002755"
     CDS             467..1477
                     /locus_tag="IVM62_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP6846660.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1592..2305)
                     /locus_tag="IVM62_002756"
     CDS             complement(1592..2305)
                     /locus_tag="IVM62_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846661.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          798 a          397 c          488 g          695 t
ORIGIN      
        1 tcatcatcga gccatttata caaatgaaga tgctttggta ataaagaaag aacaaggatt
       61 tacaggtaca gagaccaaaa gcttattctt aaaagataaa aaagataata actacgtata
      121 tctgactttt actacgaaac aaacggattt caaagcatta gggaagatcg tcggtaaaag
      181 gctttctatt gtttcatcag aacgcatgga agaacaaacg ggacaaaagc agggcgctgt
      241 ttctccgttt ggttatgaaa aacagattcc gattattatt gatgaagagc tcttatcaaa
      301 tgaaaaactt gtttttgcac caggtagacc agatcaaacg atggtcatcc aaacggttga
      361 cttaaacaaa atcatccagc tacttgagat gccgatattt ttgatgccgg caaacgaaaa
      421 aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga agcaacatga taagttatcc
      481 attattagaa agaaatgcaa cgattggggt tactgcacca tcctcaggcg tatcagagga
      541 acaccattcg ttactcgaat cagctttaaa gagaatggaa gaaaaatctt tccaagtagt
      601 tactggaaag acgctttgga cgcaagataa ggaaaaatca ggtccagcaa gaaaacgagc
      661 agatgaattc aatcaaatga tgcaagatga aaatatccaa atgatctttc cgccttgggg
      721 aggagaatta ttgattgaga ttctcgattt tgtcgatttt gatgctctac aaccaaaatg
      781 gatagtaggt tattcagata ttagtacgtt gctacttgct gtcacattaa aaacagggat
      841 agccactgca catggaacca accttattga tttaagagga gagtacttgg atgaaacgac
      901 agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa tcgattctac agcaaccttc
      961 tgaaaagtat caaaaagagt ggccccatga gcattcatca tcttctgttt tccatctcac
     1021 tgaaccaaca aattggcaat cagtcagcgg agatgcagta aggatgaagg gacgattatt
     1081 aggtggctgt attgatacga tcagtcattt aatcggtacc ccttatggag atgtccaatc
     1141 ttttaaaaga aagtataatg atggaaagcc gatcttgtgg tttctagaaa attgtgagct
     1201 atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa ctggcaggat ggtttgatgg
     1261 cagctcgggg atcatgttcg gtagaagtgc agcagaggat ttgttagatt ataaaatgaa
     1321 ggatgtatat gaagatctgt caaaggaatt aaaactaccg atcttgtatg atattgattg
     1381 tggtcatgta ccgccacaac tgacattgat caatggtgcc tatgcagaag tagcggtaga
     1441 agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa attttttcct gccttggagt
     1501 ttatcaaaga ccaacaaatc taaatagaaa agaaaatgaa agaagctgac agtattttgc
     1561 cagcttcttt ttgtatgatc tattagaaat tttaattatc cataactatc acattgatca
     1621 gctgtttcac atcatttgga ttacctgtag gaatagctac tacagatgca ttatcaataa
     1681 tttcttgagt tgtttctcta atgaacgctg gataagatgt ttgatctgca ggcatttgcc
     1741 aatgtgaagg aactggataa ctagtgattg tcccgtcatg gttacttgaa tacactaaaa
     1801 ctccctggta actgtatttc ccagtgagca taaccacatc tttcggatag actgcactcc
     1861 ctgcatcata aggggcgata ggtgttcctg caggataatg aataacatgt agttcgaaat
     1921 cacccgatgt atctctaaaa gtattacctc taattgctag ccaaacacgt gcgtactcaa
     1981 tttgtagatc agaatatcct gctaacggat ctattgactg agaagactgt gaggaagttg
     2041 tgtctattgt tgaagaagaa atatttgtct caactgtact actagatgta gaagaagtat
     2101 tgggctctga gactttactg cttgttgaag atgtattcgt ttcagtagaa gctgatttct
     2161 gagtacttga tttttcagct gaactggttt gtttgattga acttttgtct gtggtcgcag
     2221 atggatcact attttcttta ctaggagacg tacatcctac aaggaataaa gagaccaaca
     2281 ataaaataaa acctaacttt ttcatataaa atacctcatt atttttttaa taccagtata
     2341 gcataatcct gaatttttga atccgtaaaa ccttgtct
//
LOCUS       DACCIQ010000170         2351 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCIQ010000170 DACCIQ010000000
VERSION     DACCIQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.177"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..544
                     /locus_tag="IVM62_002757"
     CDS             <1..544
                     /locus_tag="IVM62_002757"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6846662.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IVM62_002758"
     CDS             complement(1017..1589)
                     /locus_tag="IVM62_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6846663.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IVM62_002759"
     CDS             complement(1605..2210)
                     /locus_tag="IVM62_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP6846664.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACCIQ010000171         2318 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCIQ010000171 DACCIQ010000000
VERSION     DACCIQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.178"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(51..1598)
                     /locus_tag="IVM62_002760"
     CDS             complement(51..1598)
                     /locus_tag="IVM62_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6846665.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKTDKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVM62_002761"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVM62_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP6846666.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IVM62_002762"
     CDS             complement(2043..2237)
                     /locus_tag="IVM62_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846667.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          649 a          479 c          416 g          774 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgttttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ttcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgctta
//
LOCUS       DACCIQ010000172         2245 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCIQ010000172 DACCIQ010000000
VERSION     DACCIQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.179"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1506
                     /locus_tag="IVM62_002763"
     CDS             <1..1506
                     /locus_tag="IVM62_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6846668.1"
                     /translation="KRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNNLFTSIF
                     QMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLGLLCIPF
                     LRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVSMLITFT
                     FQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELEKETIDD
                     IIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAIIYES
                     CIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMS
                     ISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLY
                     GVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYLSYFACS
                     YVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            1684..1905
                     /locus_tag="IVM62_002764"
     CDS             1684..1905
                     /locus_tag="IVM62_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6846669.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          753 a          235 c          430 g          827 t
ORIGIN      
        1 aaaaggaata taattactgg aacaattttt aagttatatc agattatcat tccctttttt
       61 atgcgtacac ttatgattta tcagatggga atggagtatg ctggattgaa caacctgttc
      121 acatctatat ttcaaatgtt aaatttagcg gaattgggaa taggtgctgc actaactgta
      181 agtatgtata aacctatagc agaagacaat agaaaagaga tttgtgcttt actgaacgca
      241 tataaaaagt acttttcaat tattggatgg atagtgttta cattaggatt gttatgcatt
      301 ccttttttga gatttttagt taaaggttcg attcctggaa atttaaatat gacagttttg
      361 tatttaatgt atttgataaa tactgttttg tcgtattggt tattttcata tagaaagagt
      421 ctaatatatg cacatcaaag aaatgatgta attaacgtgt ctatgctaat tacttttaca
      481 ttccaatttg taatgcaggc atatgttctg attttcttaa aaaattacta tttgtattta
      541 ttggcttcta ttttgggaca aatagttctt aacttaatgt gtgctgttgt ttctcaaaag
      601 atgtatcctg attatactcc tgttggtgag ttagagaaag aaactattga cgatattata
      661 aaaaaagtaa aaggattagt tacaaacaag gtaggcggta caatattacg atcttcagat
      721 tcaattatta tttcatcttt cttaggtctt tcagtacttg cgatttatca gaattattac
      781 tttattattt ctgccataat gagtatattt gcaataattt atgaatcatg tatagcaggt
      841 ataggtaaca gtcttattgt agaaagtcaa gaaaaaaatt atatagattt ttgtgatatt
      901 acaacaatta taggttggtt tctggcaatt agttgtgcat gctttattac attatttcag
      961 ccgttcatga ttatctgggt gggaagtgat aatcttatga gtatttcatt tgttatattt
     1021 atgatagttc attttttctt atatgaaatt gatcagttaa ttgggttata caaagatgct
     1081 gctggaatat ggtacgcaga caggtttaga ccattaatta cagcaatgat aaacattgta
     1141 ttaaatatca tcctcgttaa ttggttcgga ttatatggag ttttggcggc aaccattgtt
     1201 tcagtattgt tggttgattt accctggtta ttatataatg ttttccaaaa tatatttaaa
     1261 tttgaatcgg caggtaaata tgtttggaag ctcataagtt tgtgtgtaat agcttttgtt
     1321 gcttcttatt taagttattt tgcttgttcg tatgttaata tcggaaattt caaagtgata
     1381 agtttagtga taaatttagt tatatcggtt gtcatatcga ttttagttat tcttctgttt
     1441 aatatccgta ataagaagtt taagtatatt ttgaaagttc ttacgtcggt attggtaaaa
     1501 agatgagaag agacatcaga atgatatata aagtaactac aatttttagg ctgccgaata
     1561 ggcgaacgat gtatagtttt tgttccaacg aagactcttg ttgaaattgg agatgatatg
     1621 aaggagttac aattttgact catgtttatg attgatcggt tttaaaagta gtatggagaa
     1681 gctttgggat catcggagag gatcaaaata ggtaataatg tttttatagg aatgaagtct
     1741 agaatactaa agggtgtaca tataggaaat aatgtagtta taggtgcaaa tagtttagta
     1801 aacaaggacg tatcagacga ttgtgttgtt gcaggaaatc tagcaaaagt aattatgtcc
     1861 ctagatgaat attataaaaa gataaacaat atgaagaatc cgtaaaactt gtgaaactat
     1921 accgagaata atatgggaaa aatattgatg aaaaagcatt atatgagttt ttagggctgt
     1981 ttacagatgg agatgacaat ttgtcgaaga gttgggagca atgcaaaatt tagtagggga
     2041 ttataaacag actgacaagg tatttaaaga acatgcacaa gtatttcata atattgaaga
     2101 atttttgaat actgtgagat aggagttgct acattgagaa aagtatgtat ttttccaatg
     2161 taaaagatta cgagtttgaa aacgacacca taatattatt ttttaagaag atattataga
     2221 gcttaggaac ggaagtttat ttaag
//
LOCUS       DACCIQ010000173         2229 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCIQ010000173 DACCIQ010000000
VERSION     DACCIQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.180"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="IVM62_002765"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="IVM62_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP6846670.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          740 a          321 c          363 g          805 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttcta
//
LOCUS       DACCIQ010000174         2158 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCIQ010000174 DACCIQ010000000
VERSION     DACCIQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.181"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(59..436)
                     /locus_tag="IVM62_002766"
     CDS             complement(59..436)
                     /locus_tag="IVM62_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846671.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(736..1617)
                     /gene="galU"
                     /locus_tag="IVM62_002767"
     CDS             complement(736..1617)
                     /gene="galU"
                     /locus_tag="IVM62_002767"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6846672.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            2027..>2158
                     /locus_tag="IVM62_002768"
     CDS             2027..>2158
                     /locus_tag="IVM62_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6846673.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          591 a          463 c          339 g          765 t
ORIGIN      
        1 ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt
       61 accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg
      121 tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta
      181 gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt
      241 caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt
      301 ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa
      361 atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag
      421 cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt
      481 attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat
      541 acaacttaaa aaaaaattaa accattagca gaatattttc tgaatctatt cacgaaagtc
      601 tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca
      661 gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag
      721 gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc
      781 acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata
      841 tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc
      901 tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc
      961 ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa
     1021 attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc
     1081 cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac
     1141 acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt
     1201 caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc
     1261 tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat
     1321 gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt
     1381 ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt
     1441 ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat
     1501 aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc
     1561 cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcacttg
     1621 ctgttcctcc tcgtaaaata tcatttgtct aatagttttt attcaaaatg tttctagatt
     1681 ataaaattta ttgaataaca cattcctaac ggaatttgtt tcgatattgt gtgatttctc
     1741 agagtacaat ggatgtatgg aaaggatcca acgggaattt gctatttcct cctgtagcgt
     1801 ttgtctaccc tgtttagtct gttacccgaa ccactgttat cactaccaac tcattagctg
     1861 tttcgataat ggactagact ttcaaatagt agtttggaag gcagcttcta gctcttttat
     1921 caccgtattc tattaggcga ggttgtggct atacagtgag tttctgggaa atcccacagt
     1981 atcgaagcat tttccaaaat ttatatcgaa aggaggtcct tcttccatga aattatttgt
     2041 cggtattgac gttagctctg aaaaactaga tgtttgtttt ttaacagacg acaatcaatt
     2101 gtctatctta tctgaaatct ctgttgccaa tgacatcgaa ggtgcttcat tcatacga
//
LOCUS       DACCIQ010000175         2140 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCIQ010000175 DACCIQ010000000
VERSION     DACCIQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.182"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..700)
                     /locus_tag="IVM62_002769"
     CDS             complement(<1..700)
                     /locus_tag="IVM62_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6846674.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENL"
     gene            complement(711..2087)
                     /locus_tag="IVM62_002770"
     CDS             complement(711..2087)
                     /locus_tag="IVM62_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6846675.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
BASE COUNT          733 a          425 c          257 g          725 t
ORIGIN      
        1 ttaagttttc ccatgattct ttttctttat taaccaaaat aggataaaag aaaactttat
       61 tcttatttgc agcttctaga tcaccaggag aatcaccaat cattaatatc ttatccaaat
      121 cacaaccatt ttcaattaca cttcgaataa tgtcttcttt ttttccttta tcttgacaac
      181 acagttcatc aactaaggat aataattcat gtctttccca ttcttcttga atagcttcct
      241 tattagctga gcttacaata attatcttcc catatttttt cagagctttt atagtatctc
      301 tagcacttaa aaagggttta tctaatcctt tagcttgact aatctgtcga ttgacttctt
      361 ccgaccattt taaagctttt aataaatcct ctgactgaga gtattctatt tccctcttca
      421 aagatggatt agacaattct tttgttacag atacccagtg ctttaatcga ttaatcgatg
      481 gtacatcatt tttttctaat gttttaacta aacctaaaaa tctgtttatt ccacgagtct
      541 ctgaatataa attaatatta ttccaatcct gtaggaattt ttccctatga tgaacatcaa
      601 aaatctctgc agcaattggc ccgaaaaaga gattgtgttt ataagtcatt gtatccatga
      661 cacaaccatc tgaatcaata caaaatatcc aactgttcat gttttttctc ctagttatgt
      721 ttttgattta gcaaattata attttctaat actttttctt tagataatgg atatacgaaa
      781 aataaaatca atcctacaac aaagaaacaa actgctggaa tcaacgttga agagttatac
      841 agcttttgta aaacttctcc actttgtact tttgctaacg aatcataccc tattatagaa
      901 agagtccaac ctccgacacc gccagctaaa gcttgtccaa tttttcgggc aaaagaatat
      961 actgcataaa tagttccatc ttctctttgg ccagatttca cttcttgatc atcgattaca
     1021 tcagtaatat tcgcccaaat gactgtatta aaaataccta accccatata tccaacagaa
     1081 gtaagaacta agaatacata catattacta ggctttatca aaaataaaag taaatataca
     1141 atagcagaga agaacatccc tactgacgcc aattcttttt taccgtattt tttactgaaa
     1201 attaaactta caggtgaaac aagtaccaac atcaaaatag gattgataaa gttcattaaa
     1261 actattccct gtgcattatt ataataatct ggaaatacat aattgttcat actcattgtt
     1321 aaaagctgag caagcaacaa aaaaatagct gcagctatta tagaaagcaa cgctctattt
     1381 ttagctaatc cttttaaaga tttcaacaat gaaactttta actgcgattc atcactttta
     1441 acccgttcta cagttaattt ataacatatt acgtaacaaa taactgcaca cactgaaaat
     1501 actcctgcca caattgtaaa agttgatcca ccacgtacaa tttgagctcc agaactatct
     1561 cttgtataaa taaaaattgg tgttaacacg ccaatgaaca tacctgctaa ggtagctcct
     1621 atactacgaa atgttgataa cgctgtcctt tcttctgcaa cagaagtgat agctgatgcc
     1681 attgaaccgt aaggaatatt aatagatgta taaaaaatac ttccccatag tatatatgtt
     1741 acatacatat atataacttt aatactcata gaagctgatg ccattgatgt ttgatacatt
     1801 aaaaaacttg ctaaagcgac tggaccactc atccgtaaaa tccacggttt aaatttgcca
     1861 gttttagatg gttttgatcc atccacaata cgccccattg ttatgtctgt aactgcatca
     1921 ataaatcttg ctactaaaaa taatgttcct acggtagctc cgcttatccc caatactttt
     1981 gtataaaaaa tcattaaaaa agaactagca aaaatgaaag tgaaatcatt cccaaaatct
     2041 ccaaacatat atcctatttt gtctcttaga ccaaattttc tttccatata atttgcgctc
     2101 cttcctattg ttaagcgttt tcattctgtt gaactgccaa
//
LOCUS       DACCIQ010000176         2120 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCIQ010000176 DACCIQ010000000
VERSION     DACCIQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.183"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(83..1009)
                     /gene="cysK"
                     /locus_tag="IVM62_002771"
     CDS             complement(83..1009)
                     /gene="cysK"
                     /locus_tag="IVM62_002771"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP6846676.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1149..2036)
                     /locus_tag="IVM62_002772"
     CDS             complement(1149..2036)
                     /locus_tag="IVM62_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6846677.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          474 c          356 g          706 t
ORIGIN      
        1 ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct
       61 atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt
      121 caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt
      181 ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc
      241 caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca
      301 cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc
      361 ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt
      421 tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac
      481 ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg
      541 tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt
      601 tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag
      661 tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct
      721 caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc
      781 ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt
      841 ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa
      901 actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga
      961 ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct
     1021 ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc
     1081 gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaatttc tttgcctttt
     1141 tatctaaatc aaacgtttag ttcttgacca actaataaag aataactgga catagacatg
     1201 ccatttttcg ccattagatc atcaacagac atgccaaatt tttgtgcaat atcccatagc
     1261 gtatcacctg acacgactgt atatgtctga ccattatttg tatttacatc tgttgttgaa
     1321 gatgatgacg ttgaagtcgt cgtagtagtc gttgttgttg tacttgtggt tgttgtagcc
     1381 gaacctgttg atggcgtatc aaaacgggtt aaattataag tattaatcaa tgagttcaat
     1441 ttggccccat aatttggatc ggtagcataa cgacctgtca agtacgctgt tgcatcataa
     1501 aaacttgttg tattgctttt ccacacgcca gcataatgat aatcgcccgt ggaaaaactg
     1561 gttgaacgaa gaacattcgc atggtcttgg aaggattccc aataagaaga atattttcgg
     1621 aatggctcat tcatcgttac ccattgtccg tttacatatt ctttggttgc catataaacg
     1681 acctgtccgt tatagcttcc tttcacaccg aatagattat aatttggcgc actagctaat
     1741 ccggaagtgc catatgcact ttccaacaac gcttgagcga tcatgactga agcatacaag
     1801 tcattacttg cagcaacttc tgacgcagac cagccgattt gatcgataaa cgcttgagga
     1861 tcagcgactg tttgagatcc ttgttcatct gcatgaacat gttgcggtac cgctgtaacc
     1921 atcatcggcc caactaaaag actcgttcca attaaaggag caatcgcttt tttattgatc
     1981 accatttttc ttttaggtct atggtgccgg gcactgcggg tcttcagctc ttgcattcaa
     2041 attcctcctc aaaataaaaa aacttaataa aaagttctct tcttaaaaca aacttaatta
     2101 attaaattat acttttaatt
//
LOCUS       DACCIQ010000177         2062 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCIQ010000177 DACCIQ010000000
VERSION     DACCIQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.185"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            676..1008
                     /locus_tag="IVM62_002773"
     CDS             676..1008
                     /locus_tag="IVM62_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6846678.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1627..1968
                     /locus_tag="IVM62_002774"
     CDS             1627..1968
                     /locus_tag="IVM62_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6846679.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          718 a          275 c          433 g          636 t
ORIGIN      
        1 tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac
       61 gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat
      121 tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg
      181 cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttt
      241 tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga
      301 actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa
      361 tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca
      421 aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat
      481 ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc
      541 tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa
      601 aatcaattct ctatttttga aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat
      661 tgggtggttt attttatgtc atatacagga attttgagtc tcaaagatat ttgtcactac
      721 ggtaaacggt gtaccgctac agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa
      781 accgttgtgc agtgtaagaa atatataatc cagaaagaca aagtctcaga ggaaatgatt
      841 tattacattg ggaaacaaaa gcaaataatt ttaaaagatc ctatcccctt aaaagagcta
      901 tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat
      961 ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga tcggctgaat tgatcattct
     1021 cataacttgt attttatcgt ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga
     1081 acttagagtc actagaaaaa taaggagtag acttgatcta tcaacaattc ccttcgggga
     1141 gtgacggggt aggttttaac gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc
     1201 ttgtttggat agtggtgaaa tgaatttgtg taaataataa gcgcagagta aacaagaggg
     1261 aatttagaag ttagacaagt tagaatagcg tctagattaa tcatgagcag ctactaaggg
     1321 atctagactt tgacgcaaac tcatggaata aggagtaggt cttctcgtaa tggggaggac
     1381 gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata cttgcgagga cgttgattat
     1441 tggatcaatc gagcatttac cgctgtctac tagggtttct cgtttcggac gccataaacg
     1501 agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct
     1561 gaataaagtg aaaaaattaa tataaagcat agagaaaatg aaatttacgt aatggaggaa
     1621 tgacaaatga aaaattatga tccgaatata cgattgggca cacatactat taaggtttct
     1681 ttccaacgtt gggattacaa aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga
     1741 ttagatgttt tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca
     1801 attggattcg gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt
     1861 atgaatgata atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat
     1921 attgtcagct ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa
     1981 taaattttac agataaaggc ttagaaggag tcacgacgat taataaaaag gtagtattac
     2041 cttaggcatt gagtaaggaa tt
//
LOCUS       DACCIQ010000178         2051 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCIQ010000178 DACCIQ010000000
VERSION     DACCIQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.186"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            72..899
                     /gene="panB"
                     /locus_tag="IVM62_002775"
     CDS             72..899
                     /gene="panB"
                     /locus_tag="IVM62_002775"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP6846680.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            914..1762
                     /locus_tag="IVM62_002776"
     CDS             914..1762
                     /locus_tag="IVM62_002776"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP6846681.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            1772..>2051
                     /locus_tag="IVM62_002777"
     CDS             1772..>2051
                     /locus_tag="IVM62_002777"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP6846682.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCEL"
BASE COUNT          809 a          196 c          443 g          603 t
ORIGIN      
        1 atgttaaaaa atattaatag agattatagt gaattagaaa agtatttagg agagagttag
       61 gagagaaata gatgaaaaat acagcagtta catttaaaga atctaaatta agaaatgaaa
      121 aattaacaat gcttacagct tatgactatt caacagcaaa aataattgat gaagctggga
      181 taaatggaat attagtagga gattctcttg gtatggtatg tctaggacat gaagatacac
      241 tttcagtaac aatggaagac atgattcatc atactagagc agttacaaga ggagctaaga
      301 acactttagt tgttgcagat atgccattta tgtcttatca aacttcagta tatgattcag
      361 tagttaatgc aggaagatta ataaaagaag gacgagctca ggttgttaaa ttagaaggag
      421 ggatagaagt atgcgataaa atagaggcta tagtaaaggc ttctatacct gttatggcgc
      481 atataggttt aacacctcaa tctgttaatg catttggagg atttaaggta caaggaaagg
      541 acaaggaagc tgctaaagaa ttaataagag ctgctaaagc tgtagaaaaa gcaggagcat
      601 tcgctgtagt gttagaatgt gttccaacta agcttgcaga gttaatttct aaagagatat
      661 caatacctac aattggtatt ggagcaggtg cagggtgtga tggacaaatt cttgtatatc
      721 aagatatgtt ggggatgtat agtgatttta cccctaaatt tgtgaagaaa tatgctaatt
      781 tatcagaaga gatgaataaa gcatttacaa aatatataga ggaagtaaaa gatggagttt
      841 ttccaggtcc agaacatggg tttaaaataa gtgatgatgt tttagaaaaa ttatattaaa
      901 agggtcaaga aatatggtta tatttaaaac agtgaatgat gttagaagtc aagtaaaaga
      961 gtggaaaaaa caaggcttaa aggtaggatt agttcctact atgggatatt tacatgaagg
     1021 tcatgaaagt ttaattagaa aagcatcaaa agaaaatgat aaagttgttg taagtatctt
     1081 tgtaaatcca actcagtttg ggaaaaatga agatttaggt agttatccaa gagacttaga
     1141 aagagatata gaagtatgca caagaggtag agcaacagca atatttaatc ctgaagttga
     1201 agagatgtat tgtgacaatg caagtacttt tgttaatata actggattaa cagaaggttt
     1261 atgtggagct agtaggccaa tacattttag aggggtttgc actgttgtaa gtaaattatt
     1321 taacataata ccagcagata gagcttattt tggagaaaag gatgcacaac agttagctgt
     1381 aattaaaaga atggttaggg accttaatat tgatatagac gttgtagggt gtccaattat
     1441 aagggaagag gatggactag ctaaaagttc tagaaacaca tacttaagtt tagaggaaag
     1501 atcttcagct actattttaa ataaatcttt aactttagct aaggaagctt taaataatgg
     1561 agagagagac agcttaaaaa taattgagat aataagtaaa aatataaata cttataattt
     1621 ggcaaagatt gattatgtag aagttgttga ttctttatca ttacaaagag taaattatat
     1681 tgaaaaatca gttttagttg caatagcagt atttatagga aaaacaagat taatagataa
     1741 ttttacattt gaattacaat agggggctat aatgaaagtt acaatgttaa aaggaaaaat
     1801 acatagagct acagttgaac aagctgagtt agattatgta ggaagtatta ctgttgatat
     1861 gaatttatta gaagcagcag gaatatatga atatgaaaaa gtacaaatag ttgatgttaa
     1921 taatggaaat agatttgaaa cttatacaat agctggagag agaggaacag ggatgatttg
     1981 cttgaatgga gctgcagcta gatgtgtatc aactggagat aaaataattt taatggcata
     2041 ttgtgaatta a
//
LOCUS       DACCIQ010000179         2019 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCIQ010000179 DACCIQ010000000
VERSION     DACCIQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.187"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            100..570
                     /locus_tag="IVM62_002778"
     CDS             100..570
                     /locus_tag="IVM62_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6846683.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IVM62_002779"
     CDS             792..1895
                     /locus_tag="IVM62_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6846684.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DACCIQ010000180         2014 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCIQ010000180 DACCIQ010000000
VERSION     DACCIQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.188"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..223
                     /locus_tag="IVM62_002780"
     CDS             <1..223
                     /locus_tag="IVM62_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6846685.1"
                     /translation="RKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELK
                     YFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            224..976
                     /locus_tag="IVM62_002781"
     CDS             224..976
                     /locus_tag="IVM62_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP6846686.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            981..1928
                     /locus_tag="IVM62_002782"
     CDS             981..1928
                     /locus_tag="IVM62_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6846687.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          706 a          267 c          398 g          643 t
ORIGIN      
        1 aagaaaattt cagttgttta ctttagatga atgtatcaat gttgacttat taaataataa
       61 aataagtttt gaagaaaaca ttgttaaaga aatgcctgaa tctgctaatg atatgtatga
      121 gaaggagttg aaatatttct ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt
      181 atatcatgca aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa ttttatttac
      241 tatttgtggt agagctgggt ctaaggggtt taaaaataaa aatttgaaat atttggcagg
      301 aaagccccta gtccatttcg ctttagcaat gatagatatc tatcaacaaa aacatcctga
      361 acataattgc acgacttgtt tgaatacgga cagtaatgag ttagtagaat tgacgactaa
      421 gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag atactgtagg
      481 gaaaattgaa gtgatacgtg attcattctt acaactagaa agagaagata atatatttga
      541 tttaattata gatttagatt tgacttcacc tttacgtgaa gtttctaatc ttgaagaatt
      601 aatagatgaa catctaaaaa atttagataa agacgtctac ttttcagttg tttctgcaag
      661 acgtaatccc tattttaata tggtaaaaag aattgaaaat gaagttacta tagttaataa
      721 atcaagatac acatctaggc aacagacacc tgaagttttt gaaatgaatg cctcaatgta
      781 ttcttttaaa cctgaatttc taaaaaaaag taattatatt tttgatgggc aatgtgggat
      841 aataaaaatg agagattatc taattttaga tattgatagt gaagaagatt ttaaatggtt
      901 agaatttttg tttcctaaat ttttagaaga ttcttcggag cttcaagata tctataatta
      961 tttatatcgg aggtaaaagg atgagctgtt atcttatcac cggtggggct ggcttcattg
     1021 gttctacctt agccaatcat ttaggagaag aacatgtggt aatcgtcgtc gatgacttat
     1081 caatgggaaa agtagaaaat cttgatatga cgaaaaatat cacttttatt gagggagatg
     1141 tcgctgagaa atccttgatg gaagagatca tgaaagctaa taaatttgat tatatctttc
     1201 atttagcagc agttgctagt gtggcagatt cagttgcccg accagtagaa acacatcgtg
     1261 tgaattttga aagtgtatta atgattttag aattgatccg caagtatcaa cctgatttaa
     1321 aacggatcgt gtttagttct tcagcggctg tttatgggga tgaaccgact ttaccaaaaa
     1381 aagaagagtc cgtgattcgc ccattgacgc cttatgcggt tgataaattc gctgcagaac
     1441 aatatgtgct agattattgt catttatatg atgtacccgg tagcgcagtt cgttttttca
     1501 atgtctatgg tccaaatcaa aatccgaatt ctccatacag tggtgtgatt tctattctcg
     1561 tagatcgcta taagaaacaa atcgtaggag aagcaacttc atttacatta tttggtgatg
     1621 gaagccagtc tagagacttt gtttatatag atgatgtaat ccaagcatta cttttagttg
     1681 cgaatgagga agctgcactg gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac
     1741 ttttggaatt gattcatgca atcaatgaaa ttctcggtgt agaattgaca cttgaataca
     1801 aagaagagcg ttcgggtgat atccatgact ctctagcaga tattactaag atccagtcaa
     1861 ttggctatca accgaaatat ggtattttaa gtggaatgac taattatttg aaaactgaaa
     1921 tcaattaaaa catactaaga taagattaga gggtaaattc aggctctttg tcaataagga
     1981 ctgatgagtt ttgtaaaatc aaatctggga taga
//
LOCUS       DACCIQ010000181         2013 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCIQ010000181 DACCIQ010000000
VERSION     DACCIQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.189"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(66..1493)
                     /locus_tag="IVM62_002783"
     CDS             complement(66..1493)
                     /locus_tag="IVM62_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP6846688.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1486..>2013)
                     /locus_tag="IVM62_002784"
     CDS             complement(1486..>2013)
                     /locus_tag="IVM62_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6846689.1"
                     /translation="PRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQW
                     WALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLP
                     LELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLL
                     NRIDKEILKVGNIDE"
BASE COUNT          760 a          325 c          240 g          688 t
ORIGIN      
        1 tttgataaac atttaatata ttaatcaaat gtaaaggagt ccaagcaatg aaagctgtct
       61 tcatattaat tctccttttt taaatacaag taagaaatgc ttcccaaata ataccagaaa
      121 acaattgaaa tgacatcgcg cttattgaaa agtacatgag cctccaccat ttcatctata
      181 aaaatagata gtataaaaat taaaacaata ccaagaaacg cataattcac tttacttcta
      241 aaaaatgttt tgagtaaact aaagatattt aacacataga aagcatagat acatataaaa
      301 gcaattatac cacttgatac aagtacttga agaaatccat tatgcatgtt tccatgtgct
      361 ctttttagtt cattcacatc tgtagtatca agatctactt ttttcaaaaa ttttgcatcg
      421 ctattcccat agaaatcggt ctctccataa ccaaataaca aagttggtct tataacttta
      481 agcgcagctt tccaaatcgt aaaacgacca gctgaaattt cggagttatc actagaatgc
      541 tgtaatatta cttctttttc tggaagtgga gttttatctg attcatttga ctctataact
      601 tctacttttc tatcaatcga tacaggaatt gttgctaaaa aatccttagc ataattagta
      661 ccaaaagaaa aatataccaa tagaactaat aatatgctta ccatccattt gatttttaca
      721 ggtagtactt ctctaatgct ctttctaaat tttgaattgg caaacaacaa aagaaataat
      781 actaaaaaag aacaaattac tacctgggca cctctagacg aagaaagtgt aatataaaat
      841 atttgtacga taaaattcat catataaaaa acacgaatcg tttttgttat ttttgaaatt
      901 aatacactaa ttaaacttaa aataatagaa gtatatccaa acatagctcc aacatttgga
      961 cttgtataga caccaaataa ccgatgttca ataaaacctt gtcttgcaag taatcctggc
     1021 gttcctgttg ggacatgata agaaatcaaa aaaataaatt gatagataga tattagcgca
     1081 gctaaaaaaa ttatgataat gaaaacatta ttaaaataca ccattttttt ctttttttcc
     1141 tcttcacttt gtgaaaagtc tgcaggaaaa attactatca aagtaatcag caaatatcca
     1201 aaattataaa tagatggaat taaaaaattt tctctgttaa tgattatagt taagataaaa
     1261 ctaatcattg ctccaatcag tagccaagaa aaacgcattt taaaaaaatt tttctttata
     1321 aaaacctcat acaaaactat aattccaccc caagcaaaag ctattttcat cgggatatta
     1381 gttacattaa aagtactagg cataaaataa agcagacaaa tgattaaatt tattactcga
     1441 aacgcatctc gacttaataa actcttctct aaaacaggta ctattctatt catctatatt
     1501 tccaactttc aaaatttctt tatcaattct atttaatagt ttatcagagt aatccgattt
     1561 aacttttctt acaaataatt tttcactttc ttttatttga tctatatccc tttcatcaaa
     1621 ccatttgttc atggaaggat caataatatg aatatttgct aatttcgaag tttgtaaagt
     1681 agtttcttca atatttttca attcaagtgg taaatctggt cctccaataa catttttatt
     1741 tttgaacgag gaattaaaaa aaagtgtatg aaaaaatttt tcatctgggg caaacatacc
     1801 tattttaaaa ttcaggtagt cttgttgatt ttgttcatac atttgtatta attctgttag
     1861 gcattctttc gacaaagccc accattgaga accatgatac aaatcaaatt tatggtgtct
     1921 aatttttggg ggaagtttaa tcatcctcag acaacgatta attatagctc taaaaatttt
     1981 aaatgcagtt gtgttattcc tattaattct agg
//
LOCUS       DACCIQ010000182         2011 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCIQ010000182 DACCIQ010000000
VERSION     DACCIQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.190"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..580
                     /locus_tag="IVM62_002785"
     CDS             <1..580
                     /locus_tag="IVM62_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6846690.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            650..1989
                     /locus_tag="IVM62_002786"
     CDS             join(650..1091,1091..1989)
                     /locus_tag="IVM62_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP6846691.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          790 a          292 c          370 g          559 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cctctttgat
      601 gtgcacccca aaagttagac tagaaatcta attaaaaggg gtgctttttt tgcggaaata
      661 tacatttgtt tttaagaaaa aagtagtttc agactattta aataacgaag gcggctacaa
      721 atatcttgca cataaatatc aaataaatcg tacactggtt agacattggg taaggattta
      781 taactatcat ggttgggaag gcttggttgg aggtggcaaa agctacacta caaaatttaa
      841 acttgatgtt atagaatata tggaaacaaa tggtctttct atccaagaaa ctgctaaaaa
      901 atttaatatc ggttcaaata gaactctaag taaatggata gagcaatatg aagaaggcgg
      961 tgcttcttca cttgagagcc aaaaaagggg cagaaaaatt agtatgaatt ccaagctaaa
     1021 cattcctaaa aaacttaaag atgagtctct tgaagaagaa gttattcgtt taagagcaga
     1081 gaacgcatat ttaaaaaagt tagaaacctt gattcaagaa caagatttga gcaagaagaa
     1141 atcaaggtta aaacaatcct tcagctcaaa aaagaattta aattgaatct attactttca
     1201 attgcacaat tagcgaagtc aacttattat tactgggtaa aaaaattaga taagccagat
     1261 aaatatagca agataaagca agaaattaca gcgattgtaa aagaatcaag aaactcttat
     1321 ggttatcgta gagtcacttt agcgttaaag atgaaaggat atacaatcaa tcataaaaca
     1381 gttagaaaat taatgtccca aatgggactt acctgtcaaa tccgaataaa gcgatataaa
     1441 tcttataaag gaacagtagg aaaaattgcc aagaatgtgt taaaacgaaa tttttcagta
     1501 gatacaccca ataaaaaatg ggtgacagac gtcactgaat tcaagataaa aggaagaaaa
     1561 atctatctat ctcctatact tgatttattt aacggagaaa taattagtta tagtatatca
     1621 actagtccaa catataaact gattgaagaa atgcttcaac aagcaattaa aaaaaaggga
     1681 actgagggtt cactgattct acattcagat caaggatggc agtatcagat gccacaatat
     1741 caaaaaaaat taaaagagaa taacattatt caaagcatgt ctagaaaagg aaattgctta
     1801 gataattctg taatagaaaa tttttttggt gtgcttaaat cagagttttt ttatcgagaa
     1861 aaattccgat caattgagat atttcaaagt aaattaaatg agtatattag gtggtataat
     1921 aacaaaagga taaaactaaa gttaaatgga ctatctccag tagaatatcg gaaacagtcc
     1981 attaaatagt tcaacttttg gggttcagtt c
//
LOCUS       DACCIQ010000183         2001 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCIQ010000183 DACCIQ010000000
VERSION     DACCIQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.191"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..378
                     /locus_tag="IVM62_002787"
     CDS             <1..378
                     /locus_tag="IVM62_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP6846692.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IVM62_002788"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IVM62_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6846693.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2001
                     /locus_tag="IVM62_002789"
     CDS             1859..>2001
                     /locus_tag="IVM62_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6846694.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDA"
BASE COUNT          554 a          339 c          432 g          676 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt t
//
LOCUS       DACCIQ010000184         1967 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCIQ010000184 DACCIQ010000000
VERSION     DACCIQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.192"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(365..1657)
                     /locus_tag="IVM62_002790"
     CDS             complement(365..1657)
                     /locus_tag="IVM62_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6846695.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          627 a          386 c          320 g          634 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagacc
//
LOCUS       DACCIQ010000185         1964 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCIQ010000185 DACCIQ010000000
VERSION     DACCIQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.193"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(272..487)
                     /locus_tag="IVM62_002791"
     CDS             complement(272..487)
                     /locus_tag="IVM62_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846696.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(457..990)
                     /locus_tag="IVM62_002792"
     CDS             complement(457..990)
                     /locus_tag="IVM62_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6846697.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1011..1955)
                     /locus_tag="IVM62_002793"
     CDS             complement(1011..1955)
                     /locus_tag="IVM62_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP6846698.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT          484 a          331 c          336 g          813 t
ORIGIN      
        1 actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat ttttattggt
       61 ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc taattctttg
      121 tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt gttatgcttt
      181 ttacatatct catattcatt tattattctt ttctaaggtt aataatagga taatctcttt
      241 cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct acaaatactt
      301 cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt ttatttcctt
      361 tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg atagaaccga
      421 atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct gccaccattt
      481 tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag tcgttgaact
      541 attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt tttcgatttg
      601 ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta tatgagaatt
      661 attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac gcaactgatc
      721 tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat cgttgttttg
      781 ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag caagttgcaa
      841 tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca tggaagaatc
      901 taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat ctgacaattc
      961 ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg ctactggaat
     1021 aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc ggactctgtt
     1081 tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag gagttcaagt
     1141 tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc gtattctttt
     1201 tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc atgtttgtta
     1261 aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat aacaggtttt
     1321 ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc agttagttct
     1381 tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact tttaggaata
     1441 tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt tctgtattgc
     1501 tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa ttgttgtaaa
     1561 gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc gtaaattcgt
     1621 atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa cattacaaac
     1681 atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa gattttttca
     1741 taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta gttacttaaa
     1801 tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg cattctagac
     1861 acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg aattgtattt
     1921 aattcatggt ggtattttac aatctcatta gccatataat cacc
//
LOCUS       DACCIQ010000186         1960 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCIQ010000186 DACCIQ010000000
VERSION     DACCIQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.194"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..535
                     /locus_tag="IVM62_002794"
     CDS             <1..535
                     /locus_tag="IVM62_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6846699.1"
                     /translation="VVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVIL
                     SSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPV
                     DNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMA
                     DEMRRPMMLIVSAKKKM"
     gene            568..1476
                     /locus_tag="IVM62_002795"
     CDS             568..1476
                     /locus_tag="IVM62_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP6846700.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1473..1721
                     /locus_tag="IVM62_002796"
     CDS             1473..1721
                     /locus_tag="IVM62_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP6846701.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(1724..>1960)
                     /locus_tag="IVM62_002797"
     CDS             complement(1724..>1960)
                     /locus_tag="IVM62_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6846702.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT          677 a          289 c          421 g          573 t
ORIGIN      
        1 tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt
       61 tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt
      121 tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa
      181 aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt
      241 ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt
      301 cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt
      361 tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat
      421 aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc
      481 ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata
      541 tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta
      601 ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca
      661 aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat
      721 atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg
      781 caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg
      841 ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga gttagataat
      901 tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg
      961 gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat
     1021 gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag
     1081 gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg
     1141 atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag
     1201 tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg
     1261 gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta
     1321 tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca
     1381 aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca
     1441 tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc
     1501 tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     1561 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     1621 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     1681 ctgataagat aatatatctt ttaacccgtg gtgctagtta attttaatat cctaaagaat
     1741 atctcaaagt tcctccaaaa cgtcgttgtg ctcgacttaa tagaacactg taagccagta
     1801 atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa ttttgacaac
     1861 ctaatttttc taaggaagaa aaaacagtct caactgtttt tctttgtttt ccaagtaagc
     1921 tgtggtctag tttttctgat ttatccatgt ttttacgtgg
//
LOCUS       DACCIQ010000187         1879 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCIQ010000187 DACCIQ010000000
VERSION     DACCIQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.195"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..424)
                     /locus_tag="IVM62_002798"
     CDS             complement(<1..424)
                     /locus_tag="IVM62_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6846703.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IVM62_002799"
     CDS             complement(533..1564)
                     /locus_tag="IVM62_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6846704.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          577 a          318 c          324 g          660 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcc
//
LOCUS       DACCIQ010000188         1861 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCIQ010000188 DACCIQ010000000
VERSION     DACCIQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.196"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..486
                     /locus_tag="IVM62_002800"
     CDS             <1..486
                     /locus_tag="IVM62_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6846705.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(693..1094)
                     /locus_tag="IVM62_002801"
     CDS             complement(693..1094)
                     /locus_tag="IVM62_002801"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846706.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1215..1298)
                     /locus_tag="IVM62_002802"
                     /pseudo
     CDS             complement(1215..1298)
                     /locus_tag="IVM62_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1321..1656
                     /locus_tag="IVM62_002803"
                     /pseudo
     CDS             1321..1656
                     /locus_tag="IVM62_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1752..>1861
                     /locus_tag="IVM62_002804"
     CDS             1752..>1861
                     /locus_tag="IVM62_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846707.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          612 a          356 c          367 g          526 t
ORIGIN      
        1 ttaaaggaag cgatcgatca aatcaagacg gctgtcttgt atcctcataa cggattaccg
       61 attttattga tgggtgtaag cggttcagga aaaacctttt tggcagaaaa aatttatgaa
      121 tacgccaagg aacaacaggt gatagaatca aatgctccat ttatcgccta taattgtgct
      181 caatacttta atttctcgat taacaagatc gtagaaaatt taacgatcct tgatgcaatg
      241 aaggttttga gattggtaca aaaggccgtc aaagaactcg aaaaactaat ggggatcgaa
      301 ttttcaaaca atcaattgtt cttgctttac atgcactgct gctcaatgat cgaacgtatt
      361 ttacgcaaag aaagtgtaga tgaacaagca gatatcaaag aatatattca aaaagaggga
      421 cataattatg aaatatcaaa atacccggct tacaaaacga agtttgaaag tactgtaata
      481 aagtaagatt taaataagca taaacaaggt aagaaccata ggggctgtgt caagtccaaa
      541 atcttgtgta aaaaggaaat ctcaaagtac gatgttttgc cttgttgagc ctagctacgg
      601 tccaatatgc tggtaagaag tgtcaagagc aaaaagcgaa gcgtctcttg atacttctca
      661 ccagtatatt atactattgg cggcttaatg tgctaaatct cgttacgatc aaattcaaga
      721 tattttctag gtgcagaagt ccattcgtca tccttttcca tcaataaagc accgatgata
      781 cgaataacag aatcacaatt tgggaaaatt cggacaacac gttcacgacg gcgtacttct
      841 tcatttagtc tttcaagaac attcgttgtt ctcaatcgtt gacggatatt ttctggtaaa
      901 accattactt gcatggcatc ttcgaagccc tcatccaata ttttacaggc agttgcgact
      961 ttcttgtctt ccccatattt atccataata agttgtttac gttctccagc cagctcgatg
     1021 cttgccgttt tgaagatggc tttaaagtct tctttgatat cgcttgagat tttcttggag
     1081 aatttgtcgg aaatgttgcg aaggagatgt gcttggcatc tctgctagct cgcacaaaga
     1141 aggtattctt tcacagcttt tgtgagccct atatgcgcat ccgagatgac catctttact
     1201 cctgaaagac cgcgttcttt caatccgaga gtgtaaaata ttttgtgtaa atagaaaaaa
     1261 ggaagtccct tcagtagaat agagttacca caacacattc acagaaaaga ggacttccat
     1321 atgaacgatt ttactacaga aattctaaag actctagcga ataaaggcga tttgaatgaa
     1381 ttattccgtg tccatttgga aaaagctgtc aatacgcttc tcaaaacgga gttaacggct
     1441 ttcctcgatt acgaaaagta cgatcgcatt ggttttaaca cgggtaattc tcgtaacggc
     1501 tcctatgacc gtacggtcaa gaccgagtac ggggaacttc atctccagat tccgcgcgac
     1561 cgcaacggcg agttcaagca acagactgtt cctgcttata gacggacgaa tgatacctta
     1621 gaggagaccg tcattcacct cttccgaaaa ggtatggata ggctacaatt aactagtgga
     1681 taggctacaa ttaactagac agaaaaatta aggtgtgtag actagaagaa aacataccag
     1741 gaggaatttt tatgtctaag agaacacgaa gaactttttc acaagaattc aagcaacaaa
     1801 tcgtcaatct ttacttagct ggaaagccac gtgtagaaat cattcgagaa tatgaactaa
     1861 c
//
LOCUS       DACCIQ010000189         1836 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCIQ010000189 DACCIQ010000000
VERSION     DACCIQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.197"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            200..397
                     /locus_tag="IVM62_002805"
     CDS             200..397
                     /locus_tag="IVM62_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846708.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            503..985
                     /locus_tag="IVM62_002806"
     CDS             503..985
                     /locus_tag="IVM62_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846709.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1043..1639)
                     /locus_tag="IVM62_002807"
     CDS             complement(1043..1639)
                     /locus_tag="IVM62_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6846710.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          569 a          275 c          367 g          625 t
ORIGIN      
        1 gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca acagctgttt
       61 taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt taactgtaaa
      121 taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag aaaagttcca
      181 gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac aatatgcatg
      241 caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa tgacaaactg
      301 cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag atgaaaaagc
      361 aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg acgtgcggcg
      421 tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat tttctggaga
      481 agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt ttttattcgt
      541 tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc ggactgtgcg
      601 aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt ggtatacact
      661 catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa tgatttttat
      721 tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga atagctttga
      781 caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc caaaaggttc
      841 taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc tgaaattttc
      901 tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg aggcagagat
      961 tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt gtcacaacct
     1021 ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca tcgttcatgt
     1081 attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa cgtagtttcc
     1141 agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg ttgtaatgtt
     1201 gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct gttgcgattc
     1261 cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat tgttcttgta
     1321 gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca tttttgaaac
     1381 cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg cgaagtgctt
     1441 cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact gcgcgtgttg
     1501 attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa gttgttaatt
     1561 cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg tttttcaaag
     1621 tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc atttttttaa
     1681 ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata tgctcacaaa
     1741 aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa ggattttctt
     1801 agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DACCIQ010000190         1794 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCIQ010000190 DACCIQ010000000
VERSION     DACCIQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.198"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..1099)
                     /locus_tag="IVM62_002808"
     CDS             complement(<1..1099)
                     /locus_tag="IVM62_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP6846711.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNY"
     gene            complement(1170..1720)
                     /locus_tag="IVM62_002809"
                     /pseudo
     CDS             complement(1170..1720)
                     /locus_tag="IVM62_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT          568 a          304 c          239 g          683 t
ORIGIN      
        1 tatagttgtt gaattgagat actagaaatc tctccagaga ttcttcatta ggaaattgct
       61 cttttctttt ggtatatttc tttaattgtt tgttaaaacc ttcaatcaaa ttagttgaat
      121 aaatagttct tctgattgac ggagggaaat tataaaaggt taatatagca ggattcatga
      181 gcaatttaac cactcgtgga tactgctttt tccatttatc tatcataaac cttatttgat
      241 tatttgcttc ttcttttgaa cttgcttggt aaactaattt gaaatcgcta cagatttctt
      301 gtcgatcact aacacgaacc ttatgagcga tatttctaga tatatggaca caacattgtt
      361 gaaattgagc atttggataa acactatgaa tactatcgtc aataccactt aaaccatctg
      421 ttacgactaa caaaacctct tctaaaccac ggtcttttag atcttgaaga atctctttcc
      481 aaacataagc agattcagtt ggagctatag taaatcctag aacctcttta gttccatcca
      541 acctgatacc aattactata tagacagctt cttttgatac ggtttgtctt tttaagggaa
      601 tatgagtagc atccataaaa atgactgagt atttagcttc taaagttctt tctttaaatg
      661 ccgaaacatc ttcagacaca attttactca tatttgaaat agtttgtggt ttgtaataat
      721 gaccatacat tttttcgatt agatcagaga tttcggacat agtaattcct ttttgaacta
      781 gctgaataat agtggtttct aaagagtcat tggttctttt ataagcaggt aaagtttgtt
      841 gagaaaagtc tccattccta tctctaggta ttactaaatt caattcaccg tactctgttt
      901 taaaagagcg cgaataattc ccattacggg aattacctga attaaatcca gctctatcat
      961 acttttcata gtcaagaaat accgttaatt cagcttgtaa taaagcgtta atagccaatt
     1021 ctaggtgacg acgaaacaaa tcatccaaat cacctttatt gattagtatt tccataattt
     1081 ctgtagtaaa atcgttcata tggaagtcct cttttctgtg aatgtgttgt ggtaactcta
     1141 ttctacagaa gggacttcct tttttctatt tacacaaaat attttacact ctcttcaacg
     1201 atattcttgt aagtaattat gccccaacta ggatttgtgg gttcatttcc atctggagtt
     1261 aaaaaaacaa aaattttttt atagttagaa taattgcgtt ctatatatat ttggtattta
     1321 ttaagttgcc ctccagactc cgaagacaga attttatttt cgatgcacac cactatttta
     1381 tttttttccg ataccaataa aatatcaata ttcttatttt ctctgtaaac aattaaatct
     1441 tcatagctat taagtaaagc atcaaaataa ttgatagcag ctttggtatc atcattagaa
     1501 tctatatacc ttttcaaaaa cattttaaaa aactcatcag ataaattgtg ggtttcattt
     1561 ggagcaaaca aaaaagctaa aaaattacta tgtcttattt catttttaga tagcttaagt
     1621 atctgaaata tatttgtttt cccagtccat ttagatagtt ttcttcaata tcatccatat
     1681 cctctaatag attttttaaa cacttttttt catctatcat gttttttctc cttgccatta
     1741 ttctaactaa aattttacgt atttgtttct atgtctacat tatcttttta acag
//
LOCUS       DACCIQ010000191         1654 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCIQ010000191 DACCIQ010000000
VERSION     DACCIQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.200"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            138..1604
                     /locus_tag="IVM62_002810"
     CDS             138..1604
                     /locus_tag="IVM62_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP6846712.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          558 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctgt tttt
//
LOCUS       DACCIQ010000192         1651 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCIQ010000192 DACCIQ010000000
VERSION     DACCIQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.201"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            8..1643
                     /locus_tag="IVM62_002811"
                     /pseudo
     CDS             8..1643
                     /locus_tag="IVM62_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          516 a          268 c          294 g          573 t
ORIGIN      
        1 ttaagtaatg caagtaaaga aaaaaataat attgtccatt ctcctagcaa ttattgctgg
       61 actttattca atcaattatt ttagaaatgt tcatactatt tctacatcgg gggatattgc
      121 cttccatttt gctagagtca aagggttatc ttcaattttt tctggtccaa tcaattttac
      181 gacgtttaat cattatggga gcggagtgaa ttatttttat ccctacttga ccttttttcc
      241 ggcagtcatt ttttattgga taagtaataa tttaatcgtt agttatatct tatatgtttg
      301 gctattgaat gtttgtacta tcatgctgat gtttcattat ggactaaaat ttttaaaaag
      361 aatcgatgcg gcttttatat ttagctgttt atatacattt tatgggtacc gtacgattga
      421 tatttatcat cgttctgcta ttgctgaagc gattgcactt acggttatac cgatagttat
      481 gtattatgct tacgcactga tttatgagaa aaagccatgt gcaattccat tagcagtcag
      541 tatgagctta ctcatttata cacatgttct ttccactttt atgagtgtaa tagccgttgg
      601 cttactagtc ttaggcggct ttgtagtaag tgaaacaaga aaaaaagaaa gcatccggtt
      661 gattgtggag ttgtcaaagg cagtaggaat gacgattctt ttaacagcat acttttggta
      721 tccgatgttc cgacaaatca tggttcaatc gattaatgag ccagataaaa cgaccttaca
      781 acaccaagca ttaaatgtct cggatagttt gattggtgcg atgaataatg acctgacaac
      841 ctttacggta ggaatcattg gtattatttc tttgatctgt ccactcttct tttttaaagg
      901 atttaatcgc aaagaaaaag tgatttacct tagtgcggta gtctcttggc tcttatccac
      961 caaatttttt ccatggtctt tattacaaaa tacacccatt caaatgattc aatttccttg
     1021 gcgaatctta ggctttcaag tactgtttgg ctcgttgatt ttatcgttcg ttttcatgaa
     1081 gagcacatca gctaagcaaa gaaaagtagt taatataacg gcaattgttt tattactgtt
     1141 ggctgtatct gctgcaacaa aagcaaacta tgaacataaa gttgtcactc agagagatca
     1201 cttggtaatg aatcaagaaa atcttattcg ttacacgaca ctccttcccg gaggacttta
     1261 tgactatgca cccacggaag cattacaata caaagaacat ttgcagaatc atgaagtaag
     1321 aattggcaag gaatggcata ctatgcctta tcaagtgatg gattcgaaaa tagtctatac
     1381 agtacatgaa tctaaaggac aaaaaataac gcttcctgta tttgattatt ggggaacagt
     1441 cgcaaaagta aatggacaaa aaagagagat tgataatagg actagtgtcg attgaaggaa
     1501 cacaaggaga aacaatcatt gaagtttctt ccatttatcc tccaactatg aagttagctt
     1561 ttttaattag tttatgtagt tacctctttc ttttaattag ttttcttatc aggaagttac
     1621 aacatataaa aataatgatc tgattacatt t
//
LOCUS       DACCIQ010000193         1610 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCIQ010000193 DACCIQ010000000
VERSION     DACCIQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.202"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1019
                     /locus_tag="IVM62_002812"
     CDS             <1..1019
                     /locus_tag="IVM62_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP6846713.1"
                     /translation="KLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNEL
                     TDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLV
                     TQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVA
                     FCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDIN
                     YIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCH
                     SLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKH
                     DILI"
     gene            1075..1293
                     /locus_tag="IVM62_002813"
     CDS             1075..1293
                     /locus_tag="IVM62_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846714.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1364..>1610
                     /locus_tag="IVM62_002814"
     CDS             1364..>1610
                     /locus_tag="IVM62_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846715.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVV"
BASE COUNT          602 a          189 c          308 g          511 t
ORIGIN      
        1 aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt gatgttacat
       61 ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac aattgtttta
      121 tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata tgtaatttaa
      181 agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa atagttttat
      241 cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt actaatggat
      301 cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat cgatttcaag
      361 taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat ccaaacagtt
      421 ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa gtggatgttg
      481 ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa ctagtaaata
      541 ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg ggaatgcaaa
      601 atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat attaactata
      661 ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat catatcatcc
      721 gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca gacggaggat
      781 taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac agtctttcta
      841 aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa gatatagcta
      901 ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat attaaatggc
      961 ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt ttaatttagg
     1021 tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa aaaaatgatt
     1081 tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc tggtgaagct
     1141 gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc aggagtaggt
     1201 atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt agctgcagcc
     1261 gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta actgtctcct
     1321 attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat acaataattt
     1381 aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt atggatattt
     1441 aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc caacagtaag
     1501 atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa
     1561 aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag
//
LOCUS       DACCIQ010000194         1572 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCIQ010000194 DACCIQ010000000
VERSION     DACCIQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.203"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            57..674
                     /locus_tag="IVM62_002815"
     CDS             57..674
                     /locus_tag="IVM62_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6846716.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            671..1201
                     /locus_tag="IVM62_002816"
     CDS             671..1201
                     /locus_tag="IVM62_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6846717.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
BASE COUNT          574 a          179 c          305 g          514 t
ORIGIN      
        1 tatgatcatg tgtaattgga agcgataagt gtatacttga tattggagaa ataattatga
       61 taatagtgat tgcagaaatt attacagata tattatataa attatatgaa ccctttgggt
      121 ttgctgtttt attgacaatt ttaggactgg tctattttat gtatgcaaca gatggcattg
      181 ctgcaggtga gggctataaa cgtatactaa tggaacttta tcttcattta aagaaggata
      241 gaaaatatag aaaagtttgt gctttgatat tctatactgc gatgatttta tttacaacat
      301 taatcaatcg aacaatttgg ttaaatcctt tatctgatgt tatgggagac tggtggattt
      361 ataagtataa tacaacaact ggaacaaaag agttatctac agcgtgttta gaaaatatta
      421 ttctttttat tccatatgtt tatatggtaa tgtctattaa atatgaaaat ataaagaaac
      481 ccaaaatatg gtcctcaata gtatatggag gaaaaattgc attttatttt tcagcaataa
      541 ttgaattttt acaacttttt ttgaggctag gaacgtttca aatttcggat tttgtatata
      601 atacgttggg cggtatattg ggtgtaatcc tttataaact aattttgatt acaaaaagag
      661 atggtgaaag atgagactcg gattgataat aaagaatata ttaaatttac ctaaaacgat
      721 atacttcaat tataaatttc ttcatatttc agatgcgtgg agattaccta ttttggtgca
      781 tcgtaaagta cggattacac gcaaatcgag agggaaaatt gagatagtat tacaatctgg
      841 aaaaatagtt tatttgggct ttccagtatt aaatactgcg ggaaatgaag aaacgattat
      901 aagtattgaa ggaatactaa gaatacaagg gccagcgttt atcgcacaag gggctcatat
      961 tgacataagg aaaaatgcag ttttagatat tgggtcaaag tttaattcga caggaaaaat
     1021 gagtataatt tgctttaagg aaattcaatt caaagatggc tgtacagtat cgtgggatac
     1081 attatttatg gataccgatt cacatacgat ttttgaattg aatactcata ggattttgaa
     1141 taaaaataat aaaatagtta taggtaaaca ttgttggatt ggagcaaaaa gcgtgatata
     1201 gaaaggtgtt catttagcag ataatattgt tgttgcttgt aattccacgg tagtaaaaag
     1261 tcatgaaaat aataatgaga ttataggggg ggctccagtt cgaacgttga aaagaaatac
     1321 aagttggtgt ttgaacgagc caaagtgaaa ccaaataatt ataaaactat ggaagtataa
     1381 taaagttttt gagaaatttg aacgacaaga ccctaaaaaa atccatggac cattattatg
     1441 gatggacttc ccaaaaggaa tagccacttt tgagcacgta aatcccctaa taagtggatc
     1501 gacatcgctt gtgcagcaga tgatactatc aagaaaggtg tttataatgt agaacttgaa
     1561 ctaaatagat tt
//
LOCUS       DACCIQ010000195         1564 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCIQ010000195 DACCIQ010000000
VERSION     DACCIQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.204"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..453
                     /locus_tag="IVM62_002817"
     CDS             <1..453
                     /locus_tag="IVM62_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6846718.1"
                     /translation="KNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYS
                     GLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNV
                     FKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            698..>1564
                     /locus_tag="IVM62_002818"
     CDS             698..>1564
                     /locus_tag="IVM62_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6846719.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNLD"
BASE COUNT          617 a          184 c          317 g          446 t
ORIGIN      
        1 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
       61 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
      121 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
      181 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
      241 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
      301 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
      361 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
      421 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
      481 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
      541 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
      601 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgaccaa
      661 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
      721 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
      781 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
      841 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
      901 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
      961 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
     1021 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
     1081 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
     1141 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
     1201 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
     1261 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
     1321 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
     1381 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
     1441 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
     1501 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
     1561 tgat
//
LOCUS       DACCIQ010000196         1519 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCIQ010000196 DACCIQ010000000
VERSION     DACCIQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.205"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1361
                     /locus_tag="IVM62_002819"
     rRNA            <1..1361
                     /locus_tag="IVM62_002819"
                     /product="23S ribosomal RNA"
     gene            1455..>1519
                     /gene="rrf"
                     /locus_tag="IVM62_002820"
     rRNA            1455..>1519
                     /gene="rrf"
                     /locus_tag="IVM62_002820"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          429 a          324 c          447 g          319 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaaagaact
     1381 cggaaaaaaa cttatgtgac ttcaaatcca gttttgagtg aacaaaattt actcaataaa
     1441 agataacacc acagtgtggt ggcgatagcg agaaggatac acctgtaacc atgccgaaca
     1501 cagaagttaa gcttcttag
//
LOCUS       DACCIQ010000197         1509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCIQ010000197 DACCIQ010000000
VERSION     DACCIQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.206"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..333
                     /locus_tag="IVM62_002821"
     CDS             <1..333
                     /locus_tag="IVM62_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846720.1"
                     /translation="IELVKKIEGDLSYILENEIFVNKTRRLLLGRCRTCIQKAVRYAS
                     FNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQSIIGVKTLAYLS
                     GMGRNRRF"
     gene            651..1241
                     /locus_tag="IVM62_002822"
     CDS             651..1241
                     /locus_tag="IVM62_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP6846721.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          548 a          171 c          290 g          500 t
ORIGIN      
        1 attgaattag ttaaaaaaat tgaaggcgat ttaagctata tattagaaaa tgagatattt
       61 gtaaacaaaa caaggagact tttgttggga cgctgtcgta cttgtattca aaaggcagtt
      121 agatatgcta gttttaataa agaatttgat ttaaactcct atttgtctaa gatacttatg
      181 aacgatgagt tgattgcctg tattcactca tacccatata aacaaaatcc aattaaacaa
      241 cgaatattta actatttttt actaaaccaa tccattattg gggttaaaac attagcatat
      301 ttaagcggaa tgggtagaaa taggagattt taatcataaa aaactgtact atagtgaatt
      361 tttggagatt tatattccag ttatatatta atgattgtga tacttgtact attgtaaaaa
      421 ttcgttaggt ttaaataact ggaaatatcg gaaaatgggc attatacatc acggacatcc
      481 cttcttttgg aaaaaatgtt acgattttta taagtaatat tgtatccggt ggaatcaacg
      541 atgaagataa catatccatc gttgtagatt cagtagcatt gaatgatgct cttgttgcaa
      601 gaaatccgaa taaaattgtt aagtataaat tgaattaata ggagatatga atggaaatta
      661 gcagtaaaga atatttaaaa gcggttcttc gtatggaaaa gaatctatat atagaatcta
      721 aaggacaaca cacaaaatta ctaaaaataa tgaaatcaca tccggactac aacgcttggc
      781 ggtatactcg tcgaatgaga attacatctt attattattc aaagagaaag aaaaatatca
      841 tttatgcgat tatgtatatt gtgtcttgca gaagaatgaa tagtttggga agaaaatgtg
      901 gtatagagtg tggtgaaaat gtatttgatg aaggtgtaag attttaccat acacaaggaa
      961 ttgttattaa tggtaacgcc ataataggaa aaaattgtta cttatatggg aataactgta
     1021 ttggaaatga tggaatcgat cctaagtgtc ctgtaattgg aaataatgta cgtgtatgcg
     1081 tgggtgcaaa gattattggt aatgtaaaaa tagctaacaa tgtagttgtt gcggcaggag
     1141 ctattgttat caaagattgt ctagagaatg gtgcaattct tgcaggtgta ccggcaaaaa
     1201 taatagggta tgttaattta acaaaagtat ttttagaata atttttagcg aaagaaggtg
     1261 aataacgatg cgtatttcaa taaaaagatg gtcaggagca gattggacat tgttcacgtt
     1321 ggcatttttt gcaatctcca tagttcttgt atatactttt aacgccccaa gagcaatttt
     1381 atatttggga gatataatta atatctttgt gttattaaat gcacttagct ttagacgcag
     1441 aaaaggaata tatgctagca aaaatgagat gctgatagtt ttgtttttac tagtttctat
     1501 ttgtagctc
//
LOCUS       DACCIQ010000198         1474 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCIQ010000198 DACCIQ010000000
VERSION     DACCIQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.207"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(40..124)
                     /locus_tag="IVM62_002823"
     tRNA            complement(40..124)
                     /locus_tag="IVM62_002823"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Leu,seq:caa)
     gene            complement(142..213)
                     /locus_tag="IVM62_002824"
     tRNA            complement(142..213)
                     /locus_tag="IVM62_002824"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Gln,seq:ttg)
     gene            complement(223..295)
                     /locus_tag="IVM62_002825"
     tRNA            complement(223..295)
                     /locus_tag="IVM62_002825"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:His,seq:gtg)
     gene            complement(357..441)
                     /locus_tag="IVM62_002826"
     tRNA            complement(357..441)
                     /locus_tag="IVM62_002826"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Leu,seq:caa)
     gene            complement(462..532)
                     /locus_tag="IVM62_002827"
     tRNA            complement(462..532)
                     /locus_tag="IVM62_002827"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Cys,seq:gca)
     gene            complement(578..649)
                     /locus_tag="IVM62_002828"
     tRNA            complement(578..649)
                     /locus_tag="IVM62_002828"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Gln,seq:ttg)
     gene            complement(661..733)
                     /locus_tag="IVM62_002829"
     tRNA            complement(661..733)
                     /locus_tag="IVM62_002829"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(698..700),aa:His,seq:gtg)
     gene            complement(736..809)
                     /locus_tag="IVM62_002830"
     tRNA            complement(736..809)
                     /locus_tag="IVM62_002830"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(775..777),aa:Trp,seq:cca)
     gene            complement(816..896)
                     /locus_tag="IVM62_002831"
     tRNA            complement(816..896)
                     /locus_tag="IVM62_002831"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(860..862),aa:Tyr,seq:gta)
     gene            complement(898..973)
                     /locus_tag="IVM62_002832"
     tRNA            complement(898..973)
                     /locus_tag="IVM62_002832"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(938..940),aa:Phe,seq:gaa)
     gene            complement(1009..1081)
                     /locus_tag="IVM62_002833"
     tRNA            complement(1009..1081)
                     /locus_tag="IVM62_002833"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1046..1048),aa:Asp,seq:gtc)
     gene            complement(1091..1163)
                     /locus_tag="IVM62_002834"
     tRNA            complement(1091..1163)
                     /locus_tag="IVM62_002834"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:Val,seq:tac)
     gene            complement(1179..1250)
                     /locus_tag="IVM62_002835"
     tRNA            complement(1179..1250)
                     /locus_tag="IVM62_002835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1215..1217),aa:Glu,seq:ttc)
     gene            complement(1265..1354)
                     /locus_tag="IVM62_002836"
     tRNA            complement(1265..1354)
                     /locus_tag="IVM62_002836"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1318..1320),aa:Ser,seq:gga)
     gene            complement(1394..1467)
                     /locus_tag="IVM62_002837"
     tRNA            complement(1394..1467)
                     /locus_tag="IVM62_002837"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Asn,seq:gtt)
BASE COUNT          418 a          388 c          337 g          331 t
ORIGIN      
        1 tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc ggggtcgaac
       61 cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat tccgccacgc
      121 cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat gagggagtca
      181 aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg atgggaatcg
      241 aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatca
      301 taaaagatgt tgtttaattt ctcaataaaa aactaagatg aagacatctt agtcaatacc
      361 ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt ccagcgcgtc
      421 tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa ccggatttga
      481 accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg ccattatact
      541 attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg attcgaacca
      601 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaaag
      661 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
      721 tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg gttttggaga
      781 ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat tcgaaccact
      841 gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac tcctccatgg
      901 tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc tctaccaact
      961 gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg gttccgacgg
     1021 gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac
     1081 cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc
     1141 tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg gattcgaacc
     1201 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aggtaaattt
     1261 gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta catgatttcc
     1321 aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt
     1381 ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt aacagtcatg
     1441 cgctactacc aactgagcta tgccggaata acat
//
LOCUS       DACCIQ010000199         1463 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCIQ010000199 DACCIQ010000000
VERSION     DACCIQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.208"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            57..1226
                     /locus_tag="IVM62_002838"
     CDS             57..1226
                     /locus_tag="IVM62_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6846722.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          524 a          265 c          236 g          438 t
ORIGIN      
        1 aagctatttt agctttattc tttctgtaaa aaagaaagga gactaaataa actattatga
       61 aaaaaggaaa acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt
      121 ttagccttag tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac
      181 actctcagat agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg
      241 aacaacagct cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa
      301 tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac
      361 tcacgcaaca accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat
      421 tgactactct tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc
      481 tggctcaatt ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt
      541 atatgcgctc aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag
      601 tcgttcaatt tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt
      661 cattgaactt taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa
      721 aattattgac cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc
      781 aagtcaaaaa ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact
      841 ttaaaccact gacagaatct gaaacagaga tccataacga attagggact ggtaatatca
      901 gtatgtccgt ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt
      961 ccaaagactt actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg
     1021 gggtatatag cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat
     1081 ctgctatatt tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg
     1141 acaaaactat caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag
     1201 gaactactaa gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga
     1261 atagcatttg cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta
     1321 aaaatcaatt tttgctacag actttaattc caaataaaca gaacagaaag aggtgttctt
     1381 ctttctgttc tgtccaaatt attctaagat ttctctttac caacattatt tgacaaaggg
     1441 cctattaaat ctaaaatagt atc
//
LOCUS       DACCIQ010000200         1452 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCIQ010000200 DACCIQ010000000
VERSION     DACCIQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.209"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            15..425
                     /locus_tag="IVM62_002839"
     CDS             15..425
                     /locus_tag="IVM62_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6846723.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            439..795
                     /locus_tag="IVM62_002840"
     CDS             439..795
                     /locus_tag="IVM62_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP6846724.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          536 a          218 c          270 g          428 t
ORIGIN      
        1 aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag
       61 caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac
      121 cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata
      181 ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt
      241 taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga
      301 ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc
      361 gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt
      421 catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt
      481 ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa
      541 gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca
      601 gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt
      661 gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct
      721 ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt
      781 atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt
      841 aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta
      901 ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt
      961 aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc
     1021 aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga
     1081 catgataaga taactgagtt gttaaaaaag aaccacttgt gaagcttgat tctatcgaaa
     1141 aaaacttcaa aaaaagttct tgacaaataa caaatgataa gttatgataa atgagttgct
     1201 gtaagatttc gaaaaacgaa atcaaaaaaa cttttaaaaa agagttgaca attacttgtc
     1261 tctctgatat gatataaaag ttgctacggc agccaaaaac gaatcgggga acactcctga
     1321 tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag ggcgtcttga
     1381 ttcaattcaa gacaacaaac attttttaac aagcaagcaa tatgctagca aacaaattga
     1441 gcttaacaat cg
//
LOCUS       DACCIQ010000201         1424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCIQ010000201 DACCIQ010000000
VERSION     DACCIQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.210"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(150..1337)
                     /locus_tag="IVM62_002841"
     CDS             complement(150..1337)
                     /locus_tag="IVM62_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP6846725.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCIQ010000202         1379 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCIQ010000202 DACCIQ010000000
VERSION     DACCIQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.211"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            34..1368
                     /locus_tag="IVM62_002842"
     CDS             34..1368
                     /locus_tag="IVM62_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846726.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          489 a          200 c          245 g          445 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttact ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaa
//
LOCUS       DACCIQ010000203         1379 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCIQ010000203 DACCIQ010000000
VERSION     DACCIQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.212"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            191..445
                     /locus_tag="IVM62_002843"
     CDS             191..445
                     /locus_tag="IVM62_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6846727.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IVM62_002844"
     CDS             446..763
                     /locus_tag="IVM62_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6846728.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          481 a          204 c          263 g          431 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atacccgtgg
     1201 tgctagttaa ttttttccta ggtattatac aagtaatttg agtcggtcaa tttcaactga
     1261 attatctaaa ctaactgatc tacttttaga taattcagtt gaaatttatt ggtaaactgt
     1321 cgcttttctt tttaaatggc aaaaattttt aataatatat gacaatttag agatcaact
//
LOCUS       DACCIQ010000204         1336 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCIQ010000204 DACCIQ010000000
VERSION     DACCIQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.214"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..735
                     /locus_tag="IVM62_002845"
     CDS             <1..735
                     /locus_tag="IVM62_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6846729.1"
                     /translation="ASIRNDIREKKYLPGEQLPTEEELCKIYGVSKITIRKAFKLLAE
                     SGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFLAN
                     KLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYD
                     VKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRI
                     SYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(833..>1336)
                     /locus_tag="IVM62_002846"
     CDS             complement(833..>1336)
                     /locus_tag="IVM62_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6846730.1"
                     /translation="SIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQ
                     LEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKAS
                     EQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQE
                     VQVKFGI"
BASE COUNT          472 a          208 c          195 g          461 t
ORIGIN      
        1 gcttctatac gtaacgatat tcgtgaaaaa aaatatcttc caggagagca attacctaca
       61 gaagaagaat tatgtaaaat ttatggagta agtaagatta ccattcgaaa agcttttaaa
      121 cttttagcag aaagtggaac agttgaacga ttaaggggga aagggacctt tgtaactcaa
      181 aaaaaagaaa gtttaaaatt agctggagtt catggattta gcgattctct ttctacccga
      241 ggacataaaa ttagatatac tgttttacat tctaaaatta taaaggctga tgagttttta
      301 gctaataaat tacaaatcaa tgtaactgat agagtttaca atattaaacg tttaatgtgg
      361 gaagacggtg ccccgatggg aattgataat tttttcgcat cagaagaaaa atttccaact
      421 ttatttgaaa aaggtagcca atatacttca ttatataaac ttctagaaga tgactatgat
      481 gtcaaagcta cgagctctat tatggaaata aacggtttat ctgcaacacc tgagctttcc
      541 gacttattgc aatgtatagt aggagatccg ctatttattt tagaaaaggt tggcaaagat
      601 aaaaataatc aaccatttca ttattctcaa tccacagttc gttgtgatag aattagttat
      661 gttattgaag tcacagaaaa tatcacttta gatacggtgc ataaaaatga ttcagatcct
      721 aaagtaatag attgatatta aaagaaatca tacccaaagc aatcaatttt gattagattt
      781 gggcatgatt tttattttta ctaatattct ctaaatacat caaaaaactt atttatattc
      841 caaatttcac ctgtacttct tgtcgcagat atttaatatc gtaaataaca tcatctgcta
      901 gacttacatc ttgagaaaaa cgtttatctt taattgcaag aaccttcata tttgctcttt
      961 taccagcttt tataccatta taagaatctt ctatcaccag acattcttca actggaattc
     1021 tgagctgttc tgaagcttta aggaatatag taggattagg tttactttct ggcaaatctc
     1081 ttccgctaat taaacaatca aaatatgact ctattttaca agtggtaaca acttcattta
     1141 tagtatccat tggactggaa gaagcaattg ccatttttat ttttttcttt tttaaccatt
     1201 caagctgttc aattagtata ttaggaaaaa tatccaagta atcaatcttt tgcgatttcc
     1261 aatatttagt aaattttccc ttcaattcta aactgttaca tccctcattg tacttttcta
     1321 tatcatcaaa aatact
//
LOCUS       DACCIQ010000205         1308 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCIQ010000205 DACCIQ010000000
VERSION     DACCIQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.215"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(282..896)
                     /locus_tag="IVM62_002847"
     CDS             complement(282..896)
                     /locus_tag="IVM62_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6846731.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          419 a          261 c          217 g          411 t
ORIGIN      
        1 agaatccata tggattcttt cttgtctctt tttccactac tttttatttt tctgacacta
       61 catattctta taactcattt ctcataactg tgaaacaact tataaaaata acaaacttta
      121 aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac aaaaaaagta
      181 gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca tttttatgtg
      241 gaacattatt tttttaagga actttgtcaa catgtggata actattttcc acttgtgtcc
      301 tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga
      361 gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc atcatgtaaa
      421 ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc actgctatgt
      481 tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg atgaccattt
      541 tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga
      601 agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct
      661 agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac aagatccggg
      721 gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata tatctgcaat
      781 ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat cagcggctct
      841 tctatcctta atttctcttc caattggtga taatagtcaa aataacataa actcatttat
      901 ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta aggaagtaca
      961 taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag
     1021 gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg cagaccatgg
     1081 aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa ttaggtcgat
     1141 gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc
     1201 ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg tttctatctg
     1261 aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaa
//
LOCUS       DACCIQ010000206         1285 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCIQ010000206 DACCIQ010000000
VERSION     DACCIQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.216"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..936
                     /locus_tag="IVM62_002848"
     CDS             <1..936
                     /locus_tag="IVM62_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6846732.1"
                     /translation="FGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAG
                     IADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEA
                     KKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPS
                     FDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTK
                     LNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTY
                     EDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          441 a          172 c          217 g          455 t
ORIGIN      
        1 tttggaagga attttgatta tgaaatatct tacaatgaag tagtcactgt tactccaaga
       61 aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc aatgattggt
      121 attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga aaaaggattg
      181 agtatggctg ggctaaattt ttctgggtat gctgattata aagaaataca agaagggaaa
      241 gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc aacagtagga
      301 gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag tgatgaactt
      361 cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt cattgaaagt
      421 atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa taatccttca
      481 tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga aactcctaaa
      541 aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg agggataggc
      601 ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac gaagctgaat
      661 tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat cttaggttca
      721 gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata tacaatttat
      781 tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga agacagtcaa
      841 attactgcaa ttgatatgaa taaagaagac ttagatagtc ataagttaat tagttatcca
      901 attatagaaa aacaacaaat taaatatata aattagttaa tgtgttgtga ttgattattt
      961 aatatagata taatacaaaa aggcaaagat attttcaaaa cattttttct tgatgtgata
     1021 tccttgtctt ttttgtaatg tttttaactt attgcttttg caaaatttat ttacctgttt
     1081 tctggatcaa cgatagtgta tattatgcta tccttgtttg gattttagga cgtttaaaga
     1141 ggcacttttt tacataactt aatccataga ccttttaagc atgctgtgag ataataataa
     1201 agaagagttt aaagcgcacc ccaaaccact tccccataag tgtattacgc tttaaactct
     1261 tttatatttg aattcactaa gaagc
//
LOCUS       DACCIQ010000207         1269 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCIQ010000207 DACCIQ010000000
VERSION     DACCIQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.217"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            37..510
                     /locus_tag="IVM62_002849"
     CDS             37..510
                     /locus_tag="IVM62_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6846733.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            519..746
                     /locus_tag="IVM62_002850"
     CDS             519..746
                     /locus_tag="IVM62_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6846734.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          403 a          198 c          211 g          457 t
ORIGIN      
        1 tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta
       61 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
      121 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      181 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
      241 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
      301 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
      361 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
      421 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
      481 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
      541 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
      601 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
      661 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
      721 actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat
      781 ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta
      841 tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc
      901 cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag
      961 ttggatctgc ttttagtata atactttatt cattccacca gactgatagc agtttgagca
     1021 gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac
     1081 ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat
     1141 atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac tatgtagttt
     1201 tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa cattattttg
     1261 ataataatg
//
LOCUS       DACCIQ010000208         1266 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCIQ010000208 DACCIQ010000000
VERSION     DACCIQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.218"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            82..>1266
                     /locus_tag="IVM62_002851"
     CDS             82..>1266
                     /locus_tag="IVM62_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP6846735.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIE"
BASE COUNT          424 a          247 c          242 g          353 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaa
//
LOCUS       DACCIQ010000209         1237 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCIQ010000209 DACCIQ010000000
VERSION     DACCIQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.219"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1091
                     /locus_tag="IVM62_002852"
     CDS             <1..1091
                     /locus_tag="IVM62_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6846736.1"
                     /translation="LQAELTAFLDYEKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTI
                     PRDRNGEFSQQTLPAYKRTNDSLETTIIQLFQKGITMAEISKLIEKMYGHHYTPQTIS
                     NMSKLVAEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEV
                     LGFTIAPTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCV
                     HVSRNIAHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLN
                     PALLTFYNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYN
                     QKFLGRIHKGFKEIQDTLESMVLLQSFK"
     gene            complement(1121..>1237)
                     /locus_tag="IVM62_002853"
     CDS             complement(1121..>1237)
                     /locus_tag="IVM62_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6846737.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          423 a          213 c          230 g          371 t
ORIGIN      
        1 ttctacaagc agagctaact gcttttttag attacgaaaa atatgatcga aatggattca
       61 attcaggaaa ttcccgtaac gggaattatt cacgtacatt taaaacagaa tatggcgagt
      121 tgaatttaac tattcctaga gatcggaatg gcgaattttc tcaacaaaca ctaccagcat
      181 ataagcgaac caatgattca ttagaaacga ccattatcca attatttcaa aaaggcatta
      241 ctatggctga aatctccaaa ttaattgaaa aaatgtatgg tcaccactat acgccacaaa
      301 caatctccaa catgagtaaa ttggtggctg aagatgtttt agcttttaaa gaaagaacgt
      361 tagaagccaa ttattccgtt atatttatgg atgcgacgca tatccctgtg aagcgacaga
      421 ctgtttcgaa ggaagcagtg tatattacga ttggtattcg tttggatgga accaaagaag
      481 ttcttgggtt tactattgcc ccaactgagt ctgcttatat ttggaaagaa gttcttcaag
      541 atcttagaaa acgtgggtta gaagaagttt tattagtagt gacagacgga ttaagctgta
      601 ttgaagaaag tatccatagt gtgtatccga atgcccaatt tcaacaatgt tgtgtgcatg
      661 tatctagaaa tatcgctcat aaagttcgtg ttcgaggtcg aaaagaaatt tgtgaggatt
      721 tcaaattggt ttaccaagcg aattcaaaag aagaggcatt ggatcacatc gactttatga
      781 ctaggaaatg gaaaaagcag tatccaagag tcgtcaattt actcttgaat cctgccctat
      841 taacctttta taatttccct cacgccatca gacgaacaat ttattcgacg aacctgattg
      901 aaggctttaa taagcagcta aaacgatata ctcgaagaaa agaacaattc cctaatgaag
      961 aatctctaga gagattcttc atttctcaat ttaatcaata taaccaaaaa tttttaggta
     1021 gaattcacaa gggatttaaa gaaattcagg atacattaga gtcgatggtt ctgttgcaaa
     1081 gttttaaata aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata
     1141 ataccttgat ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct
     1201 tatataatcc tcggatggct tccatgcctt taatcgt
//
LOCUS       DACCIQ010000210         1210 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCIQ010000210 DACCIQ010000000
VERSION     DACCIQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.220"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            75..1115
                     /locus_tag="IVM62_002854"
     CDS             75..1115
                     /locus_tag="IVM62_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6846738.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          497 a          175 c          197 g          341 t
ORIGIN      
        1 atttatcttt gctattttcg aaaagagtag gtaattttac ataaataaaa caacgtgaaa
       61 gtagaggatc tacaatgact gattttaaat tttttaaggc ggatagagtt tacaacgaat
      121 tattttatca atttccaaaa gtctttattg tttctgacga atacaaaaaa atgaaagatt
      181 caactaagat tgcctatatg cttttaaaag caagattaga gatcgcaatc agcaaacggc
      241 aaatcgatga agaaggtaat gtttatttta cttatacgac aaatgaacta tgtagagtat
      301 taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga gttggaatcc tttggtttat
      361 tattacaaaa gcagatggga tttaacaaac agttagggaa aaataatcct aatagactat
      421 atctagcaga attaaaagtc tcagaaaatg atatctactt actcgaaaaa tttgatagag
      481 agaataggga aaacgttgat aaatcagagg gtatgaaaat catacccacc ctcgacgaaa
      541 aatcagacgc tgaatccctt ggggctcaag agggtatgaa aatcataccg tgccaaaacg
      601 ttgataaatc agagggtatg aaaatcatac cagaacttaa taataatata ttagacacta
      661 atagacacaa tatagacact gaaaaagacc gcctacaaga tcaattgttg ttagacaatt
      721 ttgagacaat tatgacaaac gacagcattg ctacgtttgt ccctgaacga gtattaaatt
      781 tgataaaaac attttcttca agttacagtg aagctcaaaa aaccgtccag actattcata
      841 atgcaaagaa aaaagctgaa atagaaagtg atatttcgat agtttttgaa gaactcgata
      901 gttattatgt caatgcagaa caagaattat acacgacact gttaaaagcc tatcaaaaat
      961 taaaaaccga aaaagtcgaa aatatccaga acctgatttt tgtctatgta aaaaattggt
     1021 ttatcgaaaa accaatagct gctaaagtat caagtgaaaa acgtttgaat tatgcaagct
     1081 ccccaagcac tattacgaaa gactggttag agtgaaactt tactgacaaa gtgattgttg
     1141 cttcatggag taacattaaa attaaaaatt ttttattatt ctaattatat tacgaatatt
     1201 acaaaataat
//
LOCUS       DACCIQ010000211         1175 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCIQ010000211 DACCIQ010000000
VERSION     DACCIQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.221"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            45..917
                     /locus_tag="IVM62_002855"
     CDS             45..917
                     /locus_tag="IVM62_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6846739.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          338 a          224 c          281 g          332 t
ORIGIN      
        1 catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta tacggtgcaa
       61 ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg
      121 aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc attgacttct
      181 tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg cctatcgata
      241 tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaattg gcatggcaaa
      301 attttgattt catcggtacc atgaaaaaag aatttccgat cccgatttct tggacaacag
      361 atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg
      421 tcgtttacta tacgatcggt acgggtgttg gcggcggtgc cttacaagaa gggcgcttta
      481 tcgaagggtt tagtcatcca gaaatggggc atatgctcgt cgtccctcat ccaaaagatg
      541 actttgctgg cagctgtcca tttcatggca actgtttaga agggatggcc gctggacccg
      601 ccatcgaaaa ccgcttaggg aaaaaaggac aagaggttgc agaagatgat ccttattggg
      661 aaatcgaagc ctcctatatt gctcaatgtg cctacaatac aaccttgatg ttttctccag
      721 atgtgatcat ttttggcggt ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg
      781 acgcatttgc aaaattggtc aatgggtatg tcgaaacacc tgagttaagc aaatatatcg
      841 tgaccccagc attagaagac aacgctggaa cgctcggttg tttggcattg gctcgagaag
      901 cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt agagaaagat
      961 acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt ttgttttact
     1021 tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa atttcgatag
     1081 ccgtaggcca cttgtattgt gcaatagttt tttacaaaaa aaagggggtg cggatatttt
     1141 tttggacctt tttaacaaat atatctttgc ttctt
//
LOCUS       DACCIQ010000212         1150 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCIQ010000212 DACCIQ010000000
VERSION     DACCIQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.223"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..381)
                     /locus_tag="IVM62_002856"
     CDS             complement(<1..381)
                     /locus_tag="IVM62_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846740.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQ"
     gene            complement(374..607)
                     /locus_tag="IVM62_002857"
     CDS             complement(374..607)
                     /locus_tag="IVM62_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846741.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(610..>1150)
                     /locus_tag="IVM62_002858"
     CDS             complement(610..>1150)
                     /locus_tag="IVM62_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6846742.1"
                     /translation="CDIIDVPDEFITQYRVVDPVSFKPIYGRSGKLETAYWGSRASER
                     QIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRGKATDWHAMVHESLNSFASPHFLP
                     LDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDILKRESQNDELTNHLRETFSESAD
                     VLKKELDTWLQGLDVTEEE"
BASE COUNT          325 a          270 c          157 g          398 t
ORIGIN      
        1 ttgattttct agtaacaact gatcgttttt ttgttgctga agcaaagtca atttttgttg
       61 gtcacgaatc atttccgtta attcagaaat atgcctatct ttttccgcta attgttcacg
      121 caaaaattca ttttcttttt ctaacgggaa atcattgcct gcatcattga ccggtaaatc
      181 accagtcaat tgaccacctt ttgaccgata aattgaccgc acaacaccaa tcaattcctt
      241 gtctaacatg ataaccccat tatctttata ccgagcaagg tcataacctg cattgaccag
      301 ttgattgcgc actgtatttt tagatatacc caactcatca gcaaggtttt ttatggtcat
      361 tttttctggc aaatcagtca ttatcttctc cgttactttc agctacccca taaacatatt
      421 taacagcatg cttagggata atgattgggg aatcaatttt attatttttc aaaatcaact
      481 catactgcgt tacatcaagt aacttaccgg acattttttt gctatccaaa aaaacaacga
      541 caacgttatc acctgttaaa ttcaccaacc aatttttctt accaaaatgc ttttgttttt
      601 ctgccattct tattcctcct ccgttacatc taacccttga agccaagtgt ctaattcttt
      661 tttcaaaaca tctgctgatt ccgaaaatgt ttctcgtaaa tgattcgtca actcatcatt
      721 ttgactttct cttttcaaaa tatctctcaa tttgtatttc attctacgct ctaaaaaccc
      781 ccacatttca gggttagcaa tcagtccaaa aatcatcacc ttatcatttg tttttacatc
      841 aagtggtaga aaatgagggc tagcaaaact atttaaactt tcatgaacca ttgcatgcca
      901 atctgttgct ttcccacgcc ttaactgcaa ttccagtcgc caccaagttt ctatttctgg
      961 tggcacaatt ttacgcttgg tttcctgttc caattttttg ttgtatagcc gaatctggcg
     1021 ttcgctggca cgactgcccc aataagctgt ttccagcttc ccagaccgcc cataaattgg
     1081 cttgaaagaa acaggatcaa ccactcgata ttgtgtaata aactcatcag gcacatcgat
     1141 aatatcgcaa
//
LOCUS       DACCIQ010000213         1138 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCIQ010000213 DACCIQ010000000
VERSION     DACCIQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.224"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(117..707)
                     /locus_tag="IVM62_002859"
     CDS             complement(117..707)
                     /locus_tag="IVM62_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP6846743.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          388 a          195 c          178 g          377 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgact
//
LOCUS       DACCIQ010000214         1107 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCIQ010000214 DACCIQ010000000
VERSION     DACCIQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.225"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1092
                     /locus_tag="IVM62_002860"
     CDS             <1..1092
                     /locus_tag="IVM62_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP6846744.1"
                     /translation="INTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGEL
                     TLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTP
                     QTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDG
                     TKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQ
                     HCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQ
                     SLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSI
                     FDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          405 a          206 c          183 g          313 t
ORIGIN      
        1 ataaacacac tcctacggac tgaattaacg gcttttttag attacgaaaa atatgatcgc
       61 actggtttta attcaggtaa ttcgagaaac ggttcttact ttcgatcaat caaaaccgaa
      121 tatggtgaat taacattgga aatacctaga gatcgtaatg gtgagtttaa acaacaaact
      181 ttaccagcct acaaaagaac aaacgataca ttggaaacca ctattatcca tttattcgaa
      241 aaaggtgtta cgatgtctga aattgctgat ttgatcgaaa aaatgtacgg tcatcactat
      301 actccacaaa ccatgtccaa catgactaaa gttctgactg aagaagtaaa tgcctttaaa
      361 tccagagcct taaatgataa gtatgtcgct atttttatgg acgctactta cattccacta
      421 aaacgtcaaa ccgtatccaa agaagcgatt tatattgcca ttggtatacg agaagacggc
      481 actaaagaag tactgagtta tgcgattgct ccaactgaat caacatacgt ttggaatgag
      541 ctgctacagg atattaactc cagaggagtt caagaagtct tgctttttat tacggacggc
      601 ttaaaaggca tgaaagatac tatccatcaa atttatccta aagcaaaata tcagcattgt
      661 tgtatccatg tatctcgtaa catcgctcat aaagtacgtg tcaaagaccg aaaagaaatc
      721 tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa taccttctta
      781 tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc actcatagaa
      841 aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac catttactca
      901 accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag aaaagagcag
      961 tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac atacaatcaa
     1021 aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt agtttcaatg
     1081 tttactgagt aactaattat tttgcag
//
LOCUS       DACCIQ010000215         1103 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCIQ010000215 DACCIQ010000000
VERSION     DACCIQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.226"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..641
                     /locus_tag="IVM62_002861"
     CDS             <1..641
                     /locus_tag="IVM62_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP6846745.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          369 a          215 c          151 g          368 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg aaaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatagat tttagagaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgcaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata taatttaaaa
      661 ttaacattga ttctcctatg aaaatatctt ttacctttct cctgtgaaaa ggaaatttcc
      721 ttttttatag tgttattgat ataatcagaa tattcatcaa actctgtatc tggagttaaa
      781 aatctacagt aacatctata agctttgcta gtaatcttct tgggttctct ctccattgtc
      841 aagcaacatc aaaacaccta cttactattc gattcttttc tgccaaaaac gagtcagcat
      901 cactcacaac ttatgtgttg ctgactccga ctagtccttt gttcaatatc aacttattaa
      961 acagatataa tatttttctt tatttcgtat actccaagac ttccatcttt ctttcatctt
     1021 atctctgaaa aaaacacagc aaagtgccct taataaatat acattagaat ttgcaaattt
     1081 tttcatctta agatatatcc tat
//
LOCUS       DACCIQ010000216         1085 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCIQ010000216 DACCIQ010000000
VERSION     DACCIQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.227"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>1085
                     /locus_tag="IVM62_002862"
     rRNA            <1..>1085
                     /locus_tag="IVM62_002862"
                     /product="23S ribosomal RNA"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DACCIQ010000217         1075 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCIQ010000217 DACCIQ010000000
VERSION     DACCIQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.228"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            553..>1075
                     /locus_tag="IVM62_002863"
     CDS             553..>1075
                     /locus_tag="IVM62_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP6846746.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVR"
BASE COUNT          379 a          200 c          179 g          317 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtgt aggcacgcta
      781 gaattaaaag tacccagaac acgtgatggc catttttcac ccacagtgtt tgaacgttat
      841 caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc aggcgtttca
      901 actcgtaaag tatcaaaaat tgtggaagaa ctttgtggta aatccgtctc taagtccttc
      961 gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa tcgtttatta
     1021 tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt acgag
//
LOCUS       DACCIQ010000218         1072 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCIQ010000218 DACCIQ010000000
VERSION     DACCIQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.229"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..524)
                     /locus_tag="IVM62_002864"
     CDS             complement(<1..524)
                     /locus_tag="IVM62_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846747.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMT"
     gene            complement(623..973)
                     /locus_tag="IVM62_002865"
     CDS             complement(623..973)
                     /locus_tag="IVM62_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP6846748.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          390 a          200 c          172 g          310 t
ORIGIN      
        1 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
       61 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
      121 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
      181 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
      241 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
      301 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
      361 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
      421 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
      481 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
      541 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
      601 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
      661 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
      721 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
      781 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
      841 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
      901 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
      961 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
     1021 taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt ga
//
LOCUS       DACCIQ010000219         1065 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCIQ010000219 DACCIQ010000000
VERSION     DACCIQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.230"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(38..994)
                     /locus_tag="IVM62_002866"
     CDS             complement(38..994)
                     /locus_tag="IVM62_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846749.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          381 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caact
//
LOCUS       DACCIQ010000220         1051 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCIQ010000220 DACCIQ010000000
VERSION     DACCIQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.231"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..621)
                     /locus_tag="IVM62_002867"
     CDS             complement(<1..621)
                     /locus_tag="IVM62_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6846750.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYL"
BASE COUNT          348 a          202 c          109 g          392 t
ORIGIN      
        1 caaatatgaa atatggaata tgatatcttc agatatataa tccctttctg acggaaactt
       61 gatattgtgc tgttgaatta tctcagatga atacagcgca tgccaaactg acattggcaa
      121 tataaaatcg actttataac tcggctcagt gccaatcatt ccaagcaaca ctttttcaat
      181 tatagcgtta tttgtaaata tcgtactatc atatcttgct ggctttgaaa taatattatt
      241 atcatcataa tattcataat atccagcata acatgtgtct gcattactcc tttttatacc
      301 atttaacatt gcttcaatat aattagctgc aacaaaatca tcagaatcaa taaaagcaac
      361 atattttcct tttacatgtt caagtcctgt atttcttgcc aatcccaagc cagcattatt
      421 tttatgaata atacgaatcc ttgaatcctt taatgcatag tcttcacata ttttattaga
      481 tgaatcctta gatccatcat ccacaattat aatatctagg ttcttatatg tttgattaca
      541 cactgattcc aaacaacttc ctacatactt ttcaacatta tatacaggta tgattacact
      601 aattagatca ctcgaattca tccttctatc ctttcactca cgttttctca attagtcaat
      661 gacggcatca acgattttct taaattctct gaaaaaaata gactctgatc ttgatgatgt
      721 gaatgacata ctatcttctt ctagaaaaat tatttccttt aaattattta ttaaatcaat
      781 aatatgatcc tcgtaagtat gctttccctt ggaataatga aaatatactt ttgtattaca
      841 caatattgca attttatttc ttaatatgat tatttcactc attcattact gccacatata
      901 cacaacttat aaccaccccc tgcaaagttt agggtaagtt atcagataat atcttcctct
      961 tcatcatcca gaatacaaaa tctatttatt tatagaaata tcattaatgt ttttatataa
     1021 tgtaccgtgc ttgtactctt gctctattac a
//
LOCUS       DACCIQ010000221         1035 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCIQ010000221 DACCIQ010000000
VERSION     DACCIQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.232"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..>1035)
                     /locus_tag="IVM62_002868"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVM62_002868"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCIQ010000222         1021 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCIQ010000222 DACCIQ010000000
VERSION     DACCIQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.233"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(114..314)
                     /locus_tag="IVM62_002869"
     CDS             complement(114..314)
                     /locus_tag="IVM62_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846751.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          292 a          219 c          129 g          381 t
ORIGIN      
        1 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
       61 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
      121 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
      181 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
      241 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
      301 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
      361 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
      421 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
      481 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
      541 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
      601 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
      661 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
      721 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
      781 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
      841 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
      901 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
      961 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
     1021 a
//
LOCUS       DACCIQ010000223         1006 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCIQ010000223 DACCIQ010000000
VERSION     DACCIQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.234"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            173..>1006
                     /locus_tag="IVM62_002870"
     CDS             173..>1006
                     /locus_tag="IVM62_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6846752.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
BASE COUNT          301 a          195 c          190 g          320 t
ORIGIN      
        1 aaaaacagct agaagaaaga ataagattct ttcttctagc tgttttttag ggctctatac
       61 tttttgatgt tggtgaactt caccaacatc ttttttgtat aaaaaaatat agaaaagaca
      121 ccctgaaatc ctttagaatt aatttggcta ccaattcata ggaggattca agatgtctca
      181 tactcagctt atcaaagata ccctaaatat tctagaccta aatatctatt tcgaggaaaa
      241 ttgtttaact aaagaaaaat acaaagggca aatttgcttg atttacaaag gaactcttct
      301 gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc ccctctagga tcattcgttg
      361 ggggacgaca accgttcggt tgttactgaa tgatgtgtct gagtatcgca cttatctgga
      421 gctgaagaaa caacgattta aatgtaagtc ttgccaaaga acattcgtag cggatacctc
      481 tgttgctgaa aaacactgtt tcattagtca aaaagttcgt tggtctgtca ttgctcgctt
      541 aaaagagaat acttccatga cggaaatcgc tcgtcagaaa aatatttcca cttcttcggt
      601 ctatcgtgtc atgaagcgat tctaccgccc attgaatcct ttcaaacaga ctttgccaaa
      661 ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa tgagttttat
      721 catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac gcttgcctta
      781 tttagaacgc tatttttctc ggttttcatt agcgactcgt gaagccgttc aattgattgt
      841 tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag cttttcccga atgctcagtt
      901 gatcatcgat cgtttccaca tcgtccaaca tattggcaga actttcttga atcatcgagt
      961 gaaagaaacc acggtacgac ttaaaggaaa ttcccactct caacga
//
LOCUS       DACCIQ010000224          987 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCIQ010000224 DACCIQ010000000
VERSION     DACCIQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.235"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            39..299
                     /locus_tag="IVM62_002871"
     CDS             39..299
                     /locus_tag="IVM62_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846753.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(661..900)
                     /locus_tag="IVM62_002872"
     CDS             complement(661..900)
                     /locus_tag="IVM62_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846754.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          356 a          142 c          181 g          308 t
ORIGIN      
        1 attttaaata atggaaaaac attaaggatg gatgaaatat gaataacgaa actatcattt
       61 taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtggtt ggagaagtaa
      121 tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac gtcgatcgca
      181 atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt tatgagttgg
      241 aaacaacaga atatctatat aactatagca acaaatatga acatacttta gtttcataaa
      301 gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca ctttatttag
      361 ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga gaatctgtct
      421 ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa ctgtcctatt
      481 ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt tcaaaaaatg
      541 caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt tgtaaaggtt
      601 gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa gatgaagaag
      661 ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta ccctgttttt
      721 tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct catttgtaga
      781 aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct ttgatgcaga
      841 agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa ttcttttcat
      901 tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat taggcaaaat
      961 caatgcaaaa ctatcaagtc ttttata
//
LOCUS       DACCIQ010000225          979 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCIQ010000225 DACCIQ010000000
VERSION     DACCIQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.236"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            346..979
                     /locus_tag="IVM62_002873"
                     /pseudo
     CDS             346..979
                     /locus_tag="IVM62_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          181 c          178 g          288 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttacct
//
LOCUS       DACCIQ010000226          937 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCIQ010000226 DACCIQ010000000
VERSION     DACCIQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.237"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..818
                     /locus_tag="IVM62_002874"
     CDS             <1..818
                     /locus_tag="IVM62_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6846755.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          164 g          257 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catcttg
//
LOCUS       DACCIQ010000227          929 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCIQ010000227 DACCIQ010000000
VERSION     DACCIQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.238"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(242..916)
                     /locus_tag="IVM62_002875"
                     /pseudo
     CDS             complement(242..916)
                     /locus_tag="IVM62_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          268 a          174 c          134 g          353 t
ORIGIN      
        1 ttttttaagt tacaataatt atctatttaa tagtcttaat tcattaaaat aaaaaactca
       61 actttacaag gatatggttt aaaaaattta tttatccaac ccaactagtt ttcgctgctt
      121 acgatttgct tcatctgcta cgtaattcat tactgtttta aattctttcg ctgaggcgtt
      181 ttctgataaa tcagcgatac ttttattata cttcttcacg atttgttgta cttgttcttt
      241 ctcagtcatg taattcctcc tccaactctt ttgaagtata ttgtattggt agtactaatt
      301 gaatattatc tgctgcataa tgcacttcag aaatgtcgtt ttgataatcc ttaatcatta
      361 tgtgaaggac tacttcagga tgtctatttt tgactttttc aatattctct ctggacttaa
      421 aaaacgcatt gataaacatt tcttttggca catgacgtcc ttcagctagt tcacgttttc
      481 gagtgaaatc ccaggcaact ttgggatctt ggtaaacata gaaaatcgta acgcgaaagt
      541 tatttttaag ggctcgaata atatttttct ctgcactaag gatagcaaaa gtatcctcta
      601 gtatgaaaga atagccatgt tccagtacat attttaatga ttgttcaact aaccaagaag
      661 aagctttctg aaaaacactt gagttctttc cattgtattc tggaaattgt gctctaaaat
      721 agtccgcatc aatcacaaca tggttgtcat aattttaagc taaacctagt gcaacctctg
      781 ttttacctgc tcctgggctt ccagccatga ataccgcatc tttttcatca tcagggattt
      841 ttcccttgat gattgtttca ataaatttat ctttattgac cctagcgtaa tctaaatact
      901 tgtctttagg ttccaagatt gcaccttct
//
LOCUS       DACCIQ010000228          914 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCIQ010000228 DACCIQ010000000
VERSION     DACCIQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.239"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            47..454
                     /locus_tag="IVM62_002876"
     CDS             47..454
                     /locus_tag="IVM62_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846756.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            468..869
                     /locus_tag="IVM62_002877"
     CDS             468..869
                     /locus_tag="IVM62_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846757.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          342 a          116 c          161 g          295 t
ORIGIN      
        1 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
       61 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      121 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      181 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      241 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      301 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      361 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      421 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      481 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      541 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      601 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      661 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      721 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
      781 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
      841 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
      901 tccaaacaaa ttga
//
LOCUS       DACCIQ010000229          908 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCIQ010000229 DACCIQ010000000
VERSION     DACCIQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.240"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            384..>908
                     /locus_tag="IVM62_002878"
     CDS             384..>908
                     /locus_tag="IVM62_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6846758.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          334 a          126 c          161 g          287 t
ORIGIN      
        1 tagataatag aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag
       61 tttctgaatg gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt
      121 agttttaaaa taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata
      181 cttaaaggag gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat
      241 ttgttgtttc ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat
      301 ttttcagcgg tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat
      361 agattggata ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc
      421 aaagtttagc tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg
      481 aagacgattt aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag
      541 agctgcaaga gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg
      601 atcgattagc aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag
      661 gtgcttctat tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa
      721 gagacttgct aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga
      781 aaaaaatcaa agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata
      841 ctggtaaaaa gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg
      901 aaattgtt
//
LOCUS       DACCIQ010000230          853 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCIQ010000230 DACCIQ010000000
VERSION     DACCIQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.241"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..462
                     /locus_tag="IVM62_002879"
     CDS             <1..462
                     /locus_tag="IVM62_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846759.1"
                     /translation="KCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRAR
                     TNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLI
                     LNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            449..>853
                     /locus_tag="IVM62_002880"
     CDS             449..>853
                     /locus_tag="IVM62_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6846760.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVI"
BASE COUNT          284 a          115 c          123 g          331 t
ORIGIN      
        1 aaatgtatct tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat
       61 attttcgcta aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat
      121 cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt
      181 gaagtcttgc tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca
      241 atttcttttt caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa
      301 ttaattttaa atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt
      361 gttcttttct gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac
      421 tgggcagaat taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa
      481 attagttaag tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt
      541 cataactatt cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa
      601 tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat
      661 taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc
      721 tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc
      781 aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc
      841 tagttttgta att
//
LOCUS       DACCIQ010000231          845 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCIQ010000231 DACCIQ010000000
VERSION     DACCIQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.242"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            113..805
                     /locus_tag="IVM62_002881"
     CDS             113..805
                     /locus_tag="IVM62_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6846761.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          395 a           95 c          128 g          227 t
ORIGIN      
        1 cctttttaaa taatttttta gacaaaaaac agaaagtgtc taaagaaagc ttgacgaaga
       61 aaataatttt taataactta tttaaggaaa ttttttgaag gagaagataa aagtgagaaa
      121 aaagattatt attaatgctg gagtaattat aggattgatg ttagtaattt tctccggaaa
      181 gcaatatgct gataatattt tattatgggg aggggaagat aatattgaaa agatcaacaa
      241 caatttaact atattagaca cagctctaga aaataaagag caaaaaatta gtattttaaa
      301 ttcacaattg tcttcaaaca accaaactct tgaacaacta agaactaata ttgaagtttc
      361 caaacaaaaa gtggcatctt tagaaaacga caagaatcaa ttagtatcag agaaaactaa
      421 tttagaaaat caattgtcag caaaagactc tgaattgcaa ggaaaacaaa atgaaattaa
      481 tgcgaaaata gacgaaatta accaaaaagt agctgaaata aatcgattaa atagcgattg
      541 gaataatcgt ttacaaaatg aaaagaacaa atttaatgaa gaacaatcta ctattaatag
      601 tttaaaccag caaattaatc aaatgttgag tgaaaaaaat aacattcaaa atcaactgca
      661 agctactcag caaaagctag aagaagaaaa gtcaaaaaat agtagtttga ataattacat
      721 tgaaaagctt caaaaagcta aaaatgatgt agaggatacc gcaaataaag cacaacaaat
      781 tgtaaatgaa catacaatcc aatagatata gttttaattt aacaaatggt atgtttggag
      841 ataat
//
LOCUS       DACCIQ010000232          825 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCIQ010000232 DACCIQ010000000
VERSION     DACCIQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.243"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            610..696
                     /locus_tag="IVM62_002882"
     CDS             610..696
                     /locus_tag="IVM62_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6846762.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT          266 a          168 c          145 g          246 t
ORIGIN      
        1 cacctcaatc caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc
       61 aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc
      121 cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta aagaggttta
      181 aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc taaaaaccac
      241 ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa aaccttgctt
      301 cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg cagtcgccta
      361 aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct gaatgcttat
      421 ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa gcgccttaca
      481 ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac accacacaag
      541 tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga ctggaaggag
      601 gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg tgtctcttta
      661 ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg acatcaacct
      721 gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa tgtttgatca
      781 tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttg
//
LOCUS       DACCIQ010000233          816 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCIQ010000233 DACCIQ010000000
VERSION     DACCIQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.244"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
BASE COUNT          274 a          131 c          103 g          308 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaa
//
LOCUS       DACCIQ010000234          811 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCIQ010000234 DACCIQ010000000
VERSION     DACCIQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.245"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IVM62_002883"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IVM62_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6846763.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACCIQ010000235          810 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCIQ010000235 DACCIQ010000000
VERSION     DACCIQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.246"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
BASE COUNT          271 a          117 c          123 g          299 t
ORIGIN      
        1 atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta
       61 cattatactg gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg
      121 tgtaatcggt aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta
      181 atcataaaag gtacttttac attcataatt gtcgctttga gattctactt tggttcacta
      241 tctgacccaa ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat
      301 aaaaactagc ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat
      361 ggcaaatttc tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag
      421 ttaaactata gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca
      481 tcgatatagt ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa
      541 actgtcagtt gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt
      601 taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat
      661 cacctcaatc attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg
      721 ccatcgacca cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg
      781 tttagcatat aaaaatatta tagagtttac
//
LOCUS       DACCIQ010000236          796 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCIQ010000236 DACCIQ010000000
VERSION     DACCIQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.247"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            370..796
                     /locus_tag="IVM62_002884"
                     /pseudo
     CDS             370..796
                     /locus_tag="IVM62_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          144 c          140 g          215 t
ORIGIN      
        1 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
       61 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
      121 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
      181 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
      241 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat
      301 cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa acacactgga
      361 ggatttttca tgtcaagacg tcaacgaaga acctattcaa aagaattcaa acaacaaatc
      421 gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta tgagcttacg
      481 ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt caaagaaaga
      541 gacaacttaa caccagaaca agcagaattg atcgcactaa gaaagaaaaa taagcaactc
      601 gaaatggaga atgatatttt aaagcaagcg gcgctgatat tcggacgaaa agacaagtaa
      661 ttgatgccaa caagcataaa tattccatat cagcgatgtg caaaattcta aatatttctc
      721 gtcaaaccta ctattatcaa gcgaaaccga tcgaaaatga gtccgactta gaagagatcg
      781 ttcaggaaga gtttat
//
LOCUS       DACCIQ010000237          789 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCIQ010000237 DACCIQ010000000
VERSION     DACCIQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.248"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..762)
                     /locus_tag="IVM62_002885"
     CDS             complement(<1..762)
                     /locus_tag="IVM62_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846764.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHI"
BASE COUNT          315 a          121 c          130 g          223 t
ORIGIN      
        1 aatatgtgtc agcatatcac taggagccat accacttaat tcttttccca gtgggacttt
       61 caattcttgt gctctagatt gttgaataaa aggaattaag aatcccgcac ttaaaaaaaa
      121 ggtgaggacg gtagctttta aaagacttaa gagtctagcg attttttggt cccaaaaata
      181 aaaagaaaga aacaacgttc ctccaataaa tacagaaacc atagcgacag aaagtaaatg
      241 agtatacact actagcgtca tgccaataga caaccagtac catttttggt aatctctaat
      301 ataaatttcg taacacccca ttagtatcaa aggcaaaaat gttaaagcga cagcttctcc
      361 caatgaagca cgatggaata tgtcaatggc acgataggcg gaaaatgtat aaataatcga
      421 aaataacagc gaaatataac gattgttttt tatttgtttc atagaaaaat aagaaacgac
      481 cattgttaaa aaagtgataa aaaaatagta gatattataa cttaaaacaa gatttcccat
      541 cattttataa aataaaaaag ctggataaag agtaagccat ggataaaaaa tattcatcat
      601 tgttccatga tgatcgaagt tattgaagtt tactggagaa ctccatacat tatctagtcc
      661 tatgattcga gataaatgaa agtaagtatc ctgattgaca ctatgataga ttcccttaga
      721 catgaataga gggatgacgt aagcaaaact tagaaataac ataaaaatga tatctattat
      781 ttggttttt
//
LOCUS       DACCIQ010000238          785 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCIQ010000238 DACCIQ010000000
VERSION     DACCIQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.249"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            167..>785
                     /locus_tag="IVM62_002886"
     CDS             167..>785
                     /locus_tag="IVM62_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6846765.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQ"
BASE COUNT          271 a          115 c          129 g          270 t
ORIGIN      
        1 attagaatta cgcctaattt gtgtctctca gggcaattct aagaacgttt caagagatag
       61 cgacactatc gttatcgaaa aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg
      121 tttagctttt gttaatatga ctttatgaaa atggaggtaa ttaaatatga tttttcctaa
      181 agttaatgaa gcaagcttga ataaaggatc cttttttcat atctatgaaa acgaagattt
      241 tgaactgtat aatatccttt cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc
      301 aattatttac aaatctattt tcaagtatgc attttttgct tccaatgatt ttcaaagaat
      361 ggagattacc gactgtatat tcgaaaactg tgatttttca ggctgtatat tgacaaaaag
      421 catgttgcat cgcgtgaaat ttgtcaattg taaattaaca ggtactaaat tgatggaaag
      481 ttatattggg aacacgttat tcgagaattg taaaatggac tatgtaaatt tatctggttc
      541 aaacataaaa gaaagtacct ttgagcactc tgtcttatca agcgcagatt ttgtagactg
      601 ttccctcact aaaataatgt tttctactaa tgatttagaa aatgctagct tttttgatac
      661 tgatttaaaa aatatcgatt taagtagcaa ctcctttgaa atgattgaag tacgctcgga
      721 acatctaaaa aattgtacgc ttaatcagtt gcaagcactt ggttttgcta gaaaattttt
      781 acaaa
//
LOCUS       DACCIQ010000239          767 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCIQ010000239 DACCIQ010000000
VERSION     DACCIQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.250"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            514..>767
                     /locus_tag="IVM62_002887"
     CDS             514..>767
                     /locus_tag="IVM62_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846766.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFN"
BASE COUNT          299 a           86 c          137 g          245 t
ORIGIN      
        1 aattttttcg ttagtgtatt aatgggtgaa agtttaatcc taatactgat tttgtgttta
       61 attgttcatg aagtgaataa acaattgtga attaaaaaaa taaaaaccta gtataatcac
      121 tttaataaat tgttggtaga aggtgatcaa aatgggagta aacgaaatga tgaaagtcag
      181 attgagtttt taaatgatac cttcctcaga acttcaaaac gaattcaaag attcgttgac
      241 aaatcttgag caagatcatt taaaaaaata ttttctcttc atttgacgaa aaatgtttag
      301 aaatcttata taagtgaaga taaagtaatt ggtgtaatta attgtagaga atagcctttg
      361 agcaattgaa gcgatacaat tatttagaaa atgaacttcc tccagcatac tacatgattc
      421 cttacgggac gatgttaaag aagaaagcaa gaaattttag aagtattagt agataaacta
      481 atgggctttt ctcaatttaa gggagtagcc caaatgttta tcaaatatgg tgcagataca
      541 gtagcaacca tgtttacaga agaatatcaa gtagactatt atgaagtaat agataaatgt
      601 gtacattttg tttatgaaaa agtgtggaat tttatgaatt ttgcgataag tgaagaaatc
      661 ttacgagtac aagaaaacag aacaggaata tttaccagag acagaaagct taatttgatt
      721 gcgcattata tacggaatcg ctggtcatca ataccaaatt ttaatta
//
LOCUS       DACCIQ010000240          760 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCIQ010000240 DACCIQ010000000
VERSION     DACCIQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.251"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>760
                     /locus_tag="IVM62_002888"
     CDS             <1..>760
                     /locus_tag="IVM62_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6846767.1"
                     /translation="TMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDR
                     YAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDL
                     QERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDD
                     FKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYST
                     NLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFD"
BASE COUNT          231 a          164 c          165 g          200 t
ORIGIN      
        1 accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcatcacta cacgccccaa
       61 accatgtcca atatgactaa atcatttacg gaagaagtca cttcatttaa ggaacgtgag
      121 cttcatgacc gttatgccgc tatttatatg gacgcaacct atatcccttt aaagcggaaa
      181 accgtcgcca aggaagccat tcatatcgca gttggcattc gcccggacgg ttcaaaggaa
      241 gtgttgagct atgcgattgc accaactgaa tctatcacga tttgggaaga gattttaata
      301 gacctccagg aacgtggcgt gaaaaacgtc ctccttttca tcacggatgg cttaaagggg
      361 atggcagggg ccgtgcagcg gttctacccc aaagcccgtt ttcaacattg ctgtgtgcac
      421 gtttcccgta atatcagtca caaagtgcgt gtcgatgatc gtaaggaagt ctgtgatgat
      481 tttaaaatgg tgtatcaagc gtcatctaaa aaggcggcat tggaagcacg tggtgctttt
      541 gcggaaaaat ggaaaaccag ttatccaaaa atggtggaat cgatcctttc gaacgattac
      601 ctgctcactt tctatgattt tccattagcc atacgcaaga ctatttactc tacgaacttg
      661 attgaatcct ttaataagca aatcaagaaa tacagccaca gaaaagagca gtttcaaaat
      721 gaagaatcac tagagcgttt cctagtatcc atctttgata
//
LOCUS       DACCIQ010000241          755 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCIQ010000241 DACCIQ010000000
VERSION     DACCIQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.252"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(597..740)
                     /locus_tag="IVM62_002889"
     CDS             complement(597..740)
                     /locus_tag="IVM62_002889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846768.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            659..>755
                     /locus_tag="IVM62_002890"
     rRNA            659..>755
                     /locus_tag="IVM62_002890"
                     /product="16S ribosomal RNA"
BASE COUNT          309 a          123 c          131 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccac
//
LOCUS       DACCIQ010000242          714 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCIQ010000242 DACCIQ010000000
VERSION     DACCIQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.253"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..368)
                     /locus_tag="IVM62_002891"
     CDS             complement(<1..368)
                     /locus_tag="IVM62_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846769.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDS"
     gene            complement(375..>714)
                     /locus_tag="IVM62_002892"
     CDS             complement(375..>714)
                     /locus_tag="IVM62_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846770.1"
                     /translation="VLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEFKL
                     ALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLS
                     YEMISKVLSL"
BASE COUNT          249 a          100 c           67 g          298 t
ORIGIN      
        1 aagctatccg aattttcttc gacgattaaa tacttaagag aatttatatc ggaaaagata
       61 gattcaagtt tactaatctc attttctaaa tctatatttt cacgtgtgat tatctcatca
      121 ctttttctta aaaagctaaa aattgcatca gttgctttac acttttccat taaagaagta
      181 tattcttcaa tttttcttct ttctttatgg tcatttttta aatcagtttc aatgtctttt
      241 ttcatttgtt ctatactgag tttgttcttt ttgttctttt gagtaaatct aaaattcaaa
      301 cacgaaaaaa gtatgttagc aataacacct ataatagcaa ataaatcatt tatactattt
      361 aatttcattt atcatcacaa acttagaact ttacttatca tctcataaga caagtttcca
      421 aattgataat tattggtaga ttttaaatca ttataatttt cgagaaattt tatataagta
      481 cctctcatcg gtttatggct attaaaagtg ttaaaatcac tatcttcaac tatttcttga
      541 aacctattta atttttcaat aaaaaattgt gcataaagtg ctaacttaaa ttcggtagag
      601 ctattaaagt caaaaccttt aaattgtata taataattat caattgaatt tatatctttt
      661 aatttatttt tactaaaagt aatatctcta tacatattta atatgtctag aaca
//
LOCUS       DACCIQ010000243          707 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCIQ010000243 DACCIQ010000000
VERSION     DACCIQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.254"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..291
                     /locus_tag="IVM62_002893"
     CDS             <1..291
                     /locus_tag="IVM62_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6846771.1"
                     /translation="RLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRK
                     QEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            363..>707
                     /locus_tag="IVM62_002894"
     CDS             363..>707
                     /locus_tag="IVM62_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846772.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          304 a           90 c          111 g          202 t
ORIGIN      
        1 agattagaac ctatggatag aataaaagaa ttgagtagtg catcggttcc atttgaattt
       61 gacgttcatg cgatgatttt ttcagaggac gctccgtctt tggaaactaa attgcataat
      121 cattttagaa aacaagaagt taataaaatt aatcaaagaa aagaattttt caaagtttca
      181 ctagacgaaa tagagaaagt tgttttagaa aattataatg gaacagtaac atttacaaaa
      241 ttagctaaag ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa
      301 aagccttcgg gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa
      361 taatgtggtt agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc
      421 ccttaactga aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt
      481 ggaatcaagc taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag
      541 aattaccact aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg
      601 taaaagaaag ttcctatcga acaactcagg tttatctatc attaatttca aaatatataa
      661 aagatgatgt acttgtaaaa aacgttaata gtaatcttat acaagat
//
LOCUS       DACCIQ010000244          705 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCIQ010000244 DACCIQ010000000
VERSION     DACCIQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.255"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>705
                     /locus_tag="IVM62_002895"
     CDS             <1..>705
                     /locus_tag="IVM62_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6846773.1"
                     /translation="ILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLV
                     IYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIY
                     SYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVV
                     SIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAI
                     VNILLNMIFIPRLGII"
BASE COUNT          219 a           87 c          131 g          268 t
ORIGIN      
        1 tgattttatc ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga
       61 cattatattt tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt
      121 tgataggttt agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa
      181 aacagtggag atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata
      241 tagttttagc acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg
      301 gaatatattc ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta
      361 ataacgcctg ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga
      421 agaaagttag taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag
      481 tttcgattga tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag
      541 tggttccgat aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg
      601 aattttatga gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta
      661 atatactatt aaacatgatt ttcataccta ggttaggaat aattg
//
LOCUS       DACCIQ010000245          696 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCIQ010000245 DACCIQ010000000
VERSION     DACCIQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.256"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..650
                     /locus_tag="IVM62_002896"
     CDS             <1..650
                     /locus_tag="IVM62_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP6846774.1"
                     /translation="ILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVF
                     EKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIE
                     HLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKI
                     STAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          252 a           85 c          124 g          235 t
ORIGIN      
        1 aaatactgga atatgttaaa aatggagata ttcgaaattt agaaaacatg gtttttaatc
       61 tgagtaatgg aataattcca agtgttagtg gagatacgat tcggtctgaa aaaaattatt
      121 ctatcattgt ttttgaaaag ttagcacaaa cgtctataac attaggaatg gatataatag
      181 aagcgtatca aagtagagat gctttgatac aagaaaacga attagctgta tcgttacctg
      241 aggtcttaaa agtgagggat tctgggattg tttattatac taaggaaatc ggaaaaacta
      301 agatagagca tctttctccc ttaatttctt ctgtcgttca attcatcggc ttaaatatct
      361 ataaaagaat tactgtaaag gaaatagcta attatttttc tgtaagtgaa actaaattaa
      421 gagaaagctt taagaatgaa atgcatatta cgatttataa ttatatctct aagcgaaaaa
      481 tttctacggc taaaataatg ttgaaatcaa accatactat cagtgaagtt tctttaggtt
      541 taggattttc agattcttca cacttttcga gagtattcaa aaagtacgca ggtgtatctc
      601 ctaagcaata tcaattaggt ttagtggaca atttcaacaa cattagctga gaggttttat
      661 tttattatga gactatatca tttacgtagt ttggag
//
LOCUS       DACCIQ010000246          694 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCIQ010000246 DACCIQ010000000
VERSION     DACCIQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.257"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(149..>694)
                     /locus_tag="IVM62_002897"
     CDS             complement(149..>694)
                     /locus_tag="IVM62_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6846775.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          207 a          124 c          121 g          242 t
ORIGIN      
        1 tctataaaat tcgttagaaa atacaatttg tagatatcca attgatttgt aaacaaaaag
       61 gagttgtcaa cttgtagtga cccccaaaag ttagactttt ttggagtgga gaaatccact
      121 ccttttttca tgctctaaaa tgatattttt acacaactgt tacggttgtt ttacgaaatt
      181 ggacgggact ttgccagttt aatttctgtt ttatccgttc attattgtag taatagatat
      241 agctatcgat ggctgatttt aattcctcga aacagttata aactttcccg tgaaaaatct
      301 cttgttttaa taagccaaaa aaattctcca taagtgaatt atctaagcag ttccctttcc
      361 gagacatgct ttgaaagatt ttgtactcct tcaatttgtt tctataagct ttcatttgat
      421 acgcccaacc ttgatctgag tgaaacgtac gacgataggg acaatcgtta gtggtctgga
      481 tagcttcctc caaaccttcc attaccgcta atgcattggg cttttcagag atacgatagg
      541 ataaaatctc actattgaac atatccataa aaggatctag atacaatttc ttttgacgga
      601 taatgccttt ggcatccact tcatggtatt taaattcagt tgtatccgtc gtgattttct
      661 gatggcagat actggtgtag aaacgacgat ggat
//
LOCUS       DACCIQ010000247          689 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCIQ010000247 DACCIQ010000000
VERSION     DACCIQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.258"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            82..>689
                     /locus_tag="IVM62_002898"
     CDS             82..>689
                     /locus_tag="IVM62_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP6846776.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DACCIQ010000248          679 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCIQ010000248 DACCIQ010000000
VERSION     DACCIQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.259"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..448)
                     /locus_tag="IVM62_002899"
     CDS             complement(<1..448)
                     /locus_tag="IVM62_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP6846777.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          202 a          136 c          103 g          238 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaat
//
LOCUS       DACCIQ010000249          677 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCIQ010000249 DACCIQ010000000
VERSION     DACCIQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.260"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            108..>677
                     /locus_tag="IVM62_002900"
     CDS             108..>677
                     /locus_tag="IVM62_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6846778.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTR"
BASE COUNT          314 a           74 c           98 g          191 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactaga
//
LOCUS       DACCIQ010000250          672 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCIQ010000250 DACCIQ010000000
VERSION     DACCIQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.261"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>672
                     /locus_tag="IVM62_002901"
     CDS             <1..>672
                     /locus_tag="IVM62_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP6846779.1"
                     /translation="RFRQYVKEIERDFQIEFDYYYLQITSIFLNSWIYQIQLTSDDKL
                     YSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALIL
                     AHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLV
                     DMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVD
                     YIYESS"
BASE COUNT          248 a           84 c          114 g          226 t
ORIGIN      
        1 tcgatttaga cagtatgtta aggaaataga gagagatttt caaatagaat ttgactatta
       61 ttatcttcaa ataacgtcta tctttttgaa ttcttggatt tatcaaatcc aattaacatc
      121 ggacgataaa ttatattcct tcatcaatga tattaatagt tggtgtagtc gagaagttca
      181 attagtcaat cgttttattc aaatagggga tatttattta acaaaaattc aaaaaaatgt
      241 tttggttatt ttatttaagt taatgtgtag taaagaattt cagcaaaaac gtttagcact
      301 gattttggct catggagatc taacggcaac ctgtatagca agtactgcta ataggatcgt
      361 tgagagatat atatacgacg ccattgatat gccactaatg acttcaagta gtgagattgc
      421 agacaaaatt aatagatact tacaagagtg taccggatat gaagaaatta ttttacttgt
      481 tgatatgggt tcactggaga aaatttatct aaatttggaa ctagacgctc aaaaaaaatt
      541 cggactacta aacaatgttt caacaagaat ggctattgat gttgcaaatc agatattatt
      601 agatttaccg cttaaacaga ttcttgaaca agtaaaagaa aatcaaaatg tggattatat
      661 ttatgaatca tc
//
LOCUS       DACCIQ010000251          646 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCIQ010000251 DACCIQ010000000
VERSION     DACCIQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.262"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            1..646
                     /locus_tag="IVM62_002902"
                     /pseudo
     CDS             1..646
                     /locus_tag="IVM62_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          254 a          112 c          121 g          159 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacag
//
LOCUS       DACCIQ010000252          640 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCIQ010000252 DACCIQ010000000
VERSION     DACCIQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.263"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            59..205
                     /locus_tag="IVM62_002903"
                     /pseudo
     CDS             59..205
                     /locus_tag="IVM62_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="IVM62_002904"
     CDS             282..611
                     /locus_tag="IVM62_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6846780.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          243 a           79 c          119 g          199 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt
//
LOCUS       DACCIQ010000253          638 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCIQ010000253 DACCIQ010000000
VERSION     DACCIQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.264"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>638
                     /locus_tag="IVM62_002905"
     CDS             <1..>638
                     /locus_tag="IVM62_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846781.1"
                     /translation="FNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAP
                     WRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMN
                     FQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEIT
                     FTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVT"
BASE COUNT          244 a           81 c           83 g          230 t
ORIGIN      
        1 atttaacttt aaaaaattag gaatcttatc taaaatcact tatataatgg gtataatatc
       61 tattctactt tgtacaaaaa tatttccatg gtttgcctta caaaatactc ctttaaatat
      121 tattcaagct ccttggcgtt ttatgatatt ttcaacattg ttcatatctt tttcagtatc
      181 tctatttatt tctccacagt tggaaaaatt ttcttataca aggagaagaa attttatact
      241 attattaata ttagctattt caattttaaa ttattcatca gtagttaaca tggtgaaaga
      301 aatgaatttt caaggaactt attctaatga acaaatggaa aataaacttg aagaatttga
      361 gaattttgat tatgtaccta taggctataa aaaagatcaa ttgcttttga aaaaccatca
      421 agttacagaa gatggtcaag ttatttatag tagcttttct ccaaataaag atgaaattac
      481 atttacgttt aaatcttcaa aaggcaaaac aatagtttta cctgtattcc ggtataaagg
      541 attaacggtt actgttaatt caaaaaatgc tacagtacta aaacaaggag gtccttttgt
      601 tacagtaaaa agtgaaagtg gaataaataa agtaacaa
//
LOCUS       DACCIQ010000254          634 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCIQ010000254 DACCIQ010000000
VERSION     DACCIQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.265"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            111..323
                     /locus_tag="IVM62_002906"
     CDS             111..323
                     /locus_tag="IVM62_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846782.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            485..>634
                     /locus_tag="IVM62_002907"
     CDS             485..>634
                     /locus_tag="IVM62_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846783.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENIS"
BASE COUNT          225 a           92 c          112 g          205 t
ORIGIN      
        1 agtgagtatc tccattcata aaataatcaa catatttaaa aaatctaatt agctttttta
       61 gtcaatctaa agttctttat tctatgatat aatgttactg tagaaagttt gtggcggttt
      121 ggcaaatcca attgaaggag cgtggtgcct atgggcttat tagcttatcg gacgtactct
      181 gtagaaagga aatgcctgat ttgttctcat tggatcaaat cattgctatt atcggagcag
      241 cagcagtagt tattactgcc gtagcgaaag ctagcgattc gattttagct tggttaaagt
      301 tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg gcgaggtaga cgagctctaa
      361 agctttattt acatgccatc cgctttgaac ggactacact gggcatgtga cagcatgccc
      421 tttcgtttat attatatctc attcttttat ataatgaaag taaataaatc atggaggata
      481 aaatatggat aaatttaata gattaacatt aataaatcag tttgaaattt taaaagcttt
      541 aaatcctaac gaagaaaaat actatgctga aaaaatagaa atactaacag aaggttacga
      601 atatcactat agtgaaatat ttgaaaatat ttct
//
LOCUS       DACCIQ010000255          601 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCIQ010000255 DACCIQ010000000
VERSION     DACCIQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.266"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(318..581)
                     /locus_tag="IVM62_002908"
     CDS             complement(318..581)
                     /locus_tag="IVM62_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP6846784.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          225 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 t
//
LOCUS       DACCIQ010000256          575 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCIQ010000256 DACCIQ010000000
VERSION     DACCIQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.267"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            312..>575
                     /locus_tag="IVM62_002909"
     CDS             312..>575
                     /locus_tag="IVM62_002909"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6846785.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DACCIQ010000257          565 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCIQ010000257 DACCIQ010000000
VERSION     DACCIQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.268"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..245)
                     /locus_tag="IVM62_002910"
     CDS             complement(<1..245)
                     /locus_tag="IVM62_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6846786.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT          209 a           93 c           74 g          189 t
ORIGIN      
        1 tcttgccata cctcctgaat ttgaccatca ggagtcgtaa tgaaaatagt atcattttca
       61 tctactaatt ctcttaagtt taaatattgt ttactagatg taaaattcga agctgctaat
      121 gacgagtctt cgcttttact ataaaatcct gttacttgta ttttatgttc ctggaaatac
      181 ttcccgaatg aacaacctac cttgccggct ccaataaaac caacgttcat aattatcacc
      241 tccataaaga agtgagacta caagtcccat tcttacgata tgagcttaaa aataaaaatt
      301 aaatcaaata tacagtatgt ttttttcaaa tatcatccat agttaattta tcataatatc
      361 ccatatttta aaataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
      421 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      481 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      541 aattatttca ggatcattta gagcg
//
LOCUS       DACCIQ010000258          549 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCIQ010000258 DACCIQ010000000
VERSION     DACCIQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.269"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            28..498
                     /locus_tag="IVM62_002911"
     CDS             28..498
                     /locus_tag="IVM62_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP6846787.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          201 a           99 c           81 g          168 t
ORIGIN      
        1 tatataaaat agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa
       61 gacggcactg tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact
      121 gcaacaaaat gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat
      181 cagacacaag gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat
      241 tcagcgactg aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat
      301 caattgattg acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa
      361 gctcctggta ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga
      421 aatggcaaag taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca
      481 attgaatccg ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct
      541 ttttgcctt
//
LOCUS       DACCIQ010000259          527 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCIQ010000259 DACCIQ010000000
VERSION     DACCIQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.270"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(60..281)
                     /locus_tag="IVM62_002912"
     CDS             complement(60..281)
                     /locus_tag="IVM62_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6846788.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          184 a           88 c          102 g          153 t
ORIGIN      
        1 tgttttcatt aagacgtatt acctaagagt tccatgactt tagtctggta gcgtgtcagc
       61 taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt tcttttttct atagtatata
      121 aatttttgca tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt
      181 ttagtgacag tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag
      241 tttgagtttg tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt
      301 ccgattacgc cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta
      361 gcatagtagc tgggatacca attcacatat tgacaaagat aacagagact tttacataaa
      421 acggatcaat caggaaagga attgctcgat ccagccccag ttttccaaca atgtgttaac
      481 tggcctagcg tcattcagca aattatgcat gatgaggagc gaaatga
//
LOCUS       DACCIQ010000260          526 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCIQ010000260 DACCIQ010000000
VERSION     DACCIQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.271"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..112
                     /locus_tag="IVM62_002913"
     CDS             <1..112
                     /locus_tag="IVM62_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6846789.1"
                     /translation="RFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            216..362
                     /locus_tag="IVM62_002914"
     CDS             216..362
                     /locus_tag="IVM62_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP6846790.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLY
                     YLSN"
     gene            complement(359..>526)
                     /locus_tag="IVM62_002915"
     CDS             complement(359..>526)
                     /locus_tag="IVM62_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6846791.1"
                     /translation="LRKDGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVF
                     LSYVNGKFCLA"
BASE COUNT          193 a           57 c          102 g          174 t
ORIGIN      
        1 aagattcact gaatatcaag aggaattaat aagaaatttg agtgaaattg gaagaactgc
       61 tggaataaat ggtttatatc gagcagatta tggagataaa tctcaatttt aatgatactt
      121 ataatattaa atatgtctca aaattttttg attagaaaaa taggtatatt gaatcagtga
      181 cataaaagta tttatagttt gtagggggaa tcgtaatgga agaaaaaaat agactaaatg
      241 ctaaacaatg ttcagatcaa gagcttaaaa aaattaaagg aggtgctgga acaaaacctc
      301 aaggaaaacc ggcatcaaat ttagtggaat gtgttttttc attatattat ttgtcaaatt
      361 aagcgagaca aaactttcca tttacgtaac tcaaaaaaac ttctaatggt gtttggtaat
      421 ttagtgattt tctagggata tggtttcttt tggacgcaac ggatgagatg aatccttgat
      481 tgacttggtt gaattccatt tcttttggta gtccatcttt tcggag
//
LOCUS       DACCIQ010000261          524 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCIQ010000261 DACCIQ010000000
VERSION     DACCIQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.272"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>524
                     /locus_tag="IVM62_002916"
     CDS             <1..>524
                     /locus_tag="IVM62_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP6846792.1"
                     /translation="IIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRA
                     NINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDL
                     FFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSY
                     GFKSFENMRIRIFL"
BASE COUNT          186 a          102 c           98 g          138 t
ORIGIN      
        1 attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat gaatgaactt
       61 cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttttgcta
      121 aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt tcgagcacca
      181 aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt ttctacgtcc
      241 ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga caaagaccct
      301 gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa
      361 aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat ctatccttac
      421 accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg agtatcctac
      481 ggatttaaat catttgagaa catgagaatt agaatctttt tgat
//
LOCUS       DACCIQ010000262          517 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCIQ010000262 DACCIQ010000000
VERSION     DACCIQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.273"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
BASE COUNT          225 a           53 c           82 g          157 t
ORIGIN      
        1 tatatccaac aaagaataag gtacaaaata caagcaagtg agaaattcag ggatgtcatt
       61 ataaaaaaac tagcgaacaa ttaaatagaa taacatccgt taattaaatg taagtatctt
      121 ttagatgaac gaaaaaacaa taaaatgctg taatattcgt gtttaaaaat aataaataaa
      181 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
      241 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      301 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      361 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      421 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      481 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DACCIQ010000263          515 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCIQ010000263 DACCIQ010000000
VERSION     DACCIQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.274"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(95..394)
                     /locus_tag="IVM62_002917"
     CDS             complement(95..394)
                     /locus_tag="IVM62_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6846793.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          159 a          103 c           62 g          191 t
ORIGIN      
        1 ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta
       61 tacttcttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg
      121 ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat
      181 aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt
      241 ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta
      301 tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta
      361 gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt
      421 cttcccacta cattctataa acaaagtata cgctacatct agattttttt aaatcattac
      481 tggattgtta aaacctttca atacactgtt atggc
//
LOCUS       DACCIQ010000264          489 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCIQ010000264 DACCIQ010000000
VERSION     DACCIQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.275"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>489
                     /locus_tag="IVM62_002918"
     CDS             <1..>489
                     /locus_tag="IVM62_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6846794.1"
                     /translation="QLKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSR
                     SRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAK
                     VGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDK
                     GY"
BASE COUNT          160 a           90 c           89 g          150 t
ORIGIN      
        1 tcaactgaag caacgcgata cagtgattat tgcttgtgtt atatggggca taattaatgg
       61 ctatactagc caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga
      121 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
      181 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      241 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      301 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      361 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      421 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      481 aggatatat
//
LOCUS       DACCIQ010000265          480 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCIQ010000265 DACCIQ010000000
VERSION     DACCIQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.276"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>480
                     /locus_tag="IVM62_002919"
     CDS             <1..>480
                     /locus_tag="IVM62_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP6846795.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     "
BASE COUNT          166 a           80 c           95 g          139 t
ORIGIN      
        1 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
       61 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
      121 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
      181 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
      241 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
      301 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
      361 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
      421 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
//
LOCUS       DACCIQ010000266          472 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCIQ010000266 DACCIQ010000000
VERSION     DACCIQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.277"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(71..265)
                     /locus_tag="IVM62_002920"
     CDS             complement(71..265)
                     /locus_tag="IVM62_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846796.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          153 a           86 c           82 g          151 t
ORIGIN      
        1 atacataaat tatcatatct gtatatgtca tttaggtatt tcagttatgt tataatagtt
       61 ttgctgtctg ctaccccggc ctaatctggc aaggggagga ggagatataa tgaaatacct
      121 ttttgatttt gtttttgcaa tcaagccact tgcagatgta gtgtgcagtt acctctgcaa
      181 atggcttgat tgcaaaattg ccaaaaaaga cagtaagcct acccgttaat tctataatat
      241 agaaacgtaa aaaaaacccc atcagcctgc acgctgatgg ggtttggtga tataatgaaa
      301 tacctctttg ttactcataa ttataccata attagtcacg aatgcaacca atagaaaaaa
      361 ctacttagaa cggtagatca tcgctaataa caatgcattg tccaccaaat ggattttgtg
      421 aagcattaaa tacattgtta ttaattgtcg ctgcttttaa ctaggcctct cc
//
LOCUS       DACCIQ010000267          457 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCIQ010000267 DACCIQ010000000
VERSION     DACCIQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.278"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..419
                     /locus_tag="IVM62_002921"
     CDS             <1..419
                     /locus_tag="IVM62_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846797.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          156 a           92 c           81 g          128 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaacagaa agaggttttc ttctttctgt tctgtcc
//
LOCUS       DACCIQ010000268          454 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCIQ010000268 DACCIQ010000000
VERSION     DACCIQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.279"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(128..>454)
                     /locus_tag="IVM62_002922"
     CDS             complement(128..>454)
                     /locus_tag="IVM62_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6846798.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          125 a          104 c           87 g          138 t
ORIGIN      
        1 gaatggcaac actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg
       61 ctacgattgc ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat
      121 cattcaatta cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct
      181 tcgccaatct ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc
      241 gtagatagtt ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct
      301 caaattggta ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg
      361 cattatcata cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa
      421 tctcatgaat ggtttggtta tcaaattcct ttcc
//
LOCUS       DACCIQ010000269          441 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCIQ010000269 DACCIQ010000000
VERSION     DACCIQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.281"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>441
                     /locus_tag="IVM62_002923"
     CDS             <1..>441
                     /locus_tag="IVM62_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6846799.1"
                     /translation="SSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLY
                     DSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAA
                     IVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLI"
BASE COUNT          138 a           62 c           73 g          168 t
ORIGIN      
        1 ctagctcctt tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc
       61 ttcgaggggc aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt
      121 ttataaagct ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt
      181 tattaattta cactcatgtt ctttctgtag ctatgactat ggtaatgatt ataattgctt
      241 taattattca attttatcgg aaaaagaaaa tagaaatctt cttaattatt aaattaataa
      301 aagctgctat agttacttta cttctttcac tagcgtttat aggtcctatg attgagcaga
      361 cattgtatca agatctgaat agaccttatg tggatatatt acaaaaaaga gcaatttatc
      421 tgggtaaaga gtttttgatt g
//
LOCUS       DACCIQ010000270          429 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCIQ010000270 DACCIQ010000000
VERSION     DACCIQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.282"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>429
                     /locus_tag="IVM62_002924"
     CDS             <1..>429
                     /locus_tag="IVM62_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAP6846800.1"
                     /translation="KNYQIKNKLICHCSGAISSDIFSNIQDYGAYGYSVHPMFSISDK
                     YNSYKKLKKSFITIEGDEKYAKFLSDFFKKLGNSTVIVSKENKSLYHAASVVSSNLVL
                     GLINTSVTYLIQCGFTEKMAIEALYPLIEFNIRNIKEKGII"
BASE COUNT          169 a           56 c           58 g          146 t
ORIGIN      
        1 aaaaattatc aaatcaaaaa caaattaatc tgccattgca gtggtgcaat atcttcagat
       61 atcttttcaa atatacagga ctatggtgct tatggctatt ctgtccatcc tatgttttca
      121 atatcagata aatataattc atacaaaaaa ttaaaaaaat cttttataac aattgaaggc
      181 gatgaaaaat atgcaaagtt tttatctgat ttcttcaaaa agttaggtaa tagtactgta
      241 attgtatcta aggagaataa atctctttat catgccgcat cagtagtatc tagtaacctt
      301 gtgctaggac ttattaatac aagcgttaca tatctaattc aatgtggatt tactgaaaaa
      361 atggcaattg aagctttata tccattaatt gaatttaata ttagaaatat aaaagaaaaa
      421 ggaattata
//
LOCUS       DACCIQ010000271          415 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCIQ010000271 DACCIQ010000000
VERSION     DACCIQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.283"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..339)
                     /locus_tag="IVM62_002925"
     CDS             complement(<1..339)
                     /locus_tag="IVM62_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846801.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACCIQ010000272          410 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCIQ010000272 DACCIQ010000000
VERSION     DACCIQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.284"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            133..351
                     /locus_tag="IVM62_002926"
                     /pseudo
     CDS             133..351
                     /locus_tag="IVM62_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          140 a           59 c           72 g          139 t
ORIGIN      
        1 tagatacaat atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga
       61 gttactttca atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt
      121 gatgaaattg ccattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat
      181 tggtctaatg gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa
      241 aataaagtta ttccagtatt gctatatgga acacataaca atttttatga attagccgag
      301 aacactcgca actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta
      361 tatcaatcaa ccgaccgaca tatgttaaat ctatatataa taaaaatacc
//
LOCUS       DACCIQ010000273          407 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCIQ010000273 DACCIQ010000000
VERSION     DACCIQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.285"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            17..>407
                     /locus_tag="IVM62_002927"
     CDS             17..>407
                     /locus_tag="IVM62_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6846802.1"
                     /translation="MFIVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTY
                     PTGTIVGDMIGGVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALF
                     MSALAELKVFIPELFIIFVAIVFTLKVD"
BASE COUNT          130 a           47 c           67 g          163 t
ORIGIN      
        1 acgaaataac actcgtatgt tcatagtata tataaattgt gcaacatatt taacaattaa
       61 agattactat aaagtattcg ctataattga aaaattattc tggatttcac tgccactttg
      121 tttaattgaa agattcatgg tcacctatcc aactggaaca attgtaggag atatgatagg
      181 tggagttttt tggaattatt caggttgtaa tatgccattg aatttaattt tgtgtgtgac
      241 agttactgtt gttacaatcg gatatttcaa gggagaaaaa aatttcatcc attttatttt
      301 atgtgtttgt gcagcacttt ttatgtctgc attagctgaa ttgaaagttt ttattcctga
      361 attatttata atatttgttg caatagtatt cactttaaaa gttgatt
//
LOCUS       DACCIQ010000274          404 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCIQ010000274 DACCIQ010000000
VERSION     DACCIQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.286"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>404
                     /locus_tag="IVM62_002928"
     CDS             <1..>404
                     /locus_tag="IVM62_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846803.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACCIQ010000275          398 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCIQ010000275 DACCIQ010000000
VERSION     DACCIQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.287"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
BASE COUNT          153 a           59 c           85 g          101 t
ORIGIN      
        1 aaaatattaa gtattgttaa atagtgataa ttagtaatat acgaattata agatacattt
       61 tatatttaat gtatgtaaat aaataaaatg actgacggat attgaatagt aataaataac
      121 aataattcag acggatacag tgagtgaagt agcttgctac gaacgaacag gcagaatgaa
      181 acggtagttt cattagagca agtcgcttgc gacgtagcgt taccactgat gtaactggca
      241 agttacatag agagagtttg cttgcaaacg aacgttaccc acttgaatag aaaacaaaaa
      301 taacatatta gtgaattttg aataggagcc acaactcaac caaccgcttg cggtgggtga
      361 gtcccacgga ggaaaagacg aacgaacggt cgcgtagc
//
LOCUS       DACCIQ010000276          390 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCIQ010000276 DACCIQ010000000
VERSION     DACCIQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.288"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
BASE COUNT          133 a           61 c           52 g          144 t
ORIGIN      
        1 aaaacatata aaaagatatg tatgtattct ttctgaaatt aagttcattt ctatgcgatg
       61 aagcatgttt tcaaaatatt accctttctt agattaaact tcagagtatt caaataacgg
      121 aataaggagc cttatcccgc attactgaat gagactgtga cataagtaaa aaggtgactt
      181 caaattcctt cttcggaatt tgaaatcacc ttctttataa ttataaagta tcggtttcac
      241 agcatttctg aatgattcct agaatcttaa acgaatgatt ggagttatca acattttttc
      301 actcgttttt agcgaaacaa aaaatgacat ttgtcacagc ctcattaaat tattgattct
      361 ttttctttat aattgaattg tccatattta
//
LOCUS       DACCIQ010000277          388 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCIQ010000277 DACCIQ010000000
VERSION     DACCIQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.289"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
BASE COUNT          158 a           45 c           61 g          124 t
ORIGIN      
        1 aagcgcatac tttatgggtg attaaatgtg attttaatac atataccaat taaaagaaaa
       61 cttttttaga tttttactta caaatttata aaaaaacaag ataattgata ctttgatttt
      121 gcgaacattt tatctttccg cctaattcag ctgcaatatc atgattatat cagtaacaaa
      181 tcgctttttt agctaattta agcatcaaat aattattctt aatcagtgaa aaaaataacg
      241 aataaatgga ttttgggaat aggtaaaaac ttgatcggtt tctggataaa aataacatga
      301 aaaaggaaat ataaaataac atggagcgtg ggaggaataa agaaatttta cactcgaggt
      361 gcgtaggaat gtaaagaaac ttaacacg
//
LOCUS       DACCIQ010000278          385 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCIQ010000278 DACCIQ010000000
VERSION     DACCIQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.290"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>385
                     /locus_tag="IVM62_002929"
     CDS             <1..>385
                     /locus_tag="IVM62_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6846804.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELK"
BASE COUNT          154 a           44 c           57 g          130 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaat
//
LOCUS       DACCIQ010000279          365 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCIQ010000279 DACCIQ010000000
VERSION     DACCIQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.292"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>365
                     /locus_tag="IVM62_002930"
     CDS             <1..>365
                     /locus_tag="IVM62_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6846805.1"
                     /translation="TRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTA
                     CNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEK
                     KDASLVKEAFGRIPYSLTDV"
BASE COUNT          112 a           69 c           86 g           98 t
ORIGIN      
        1 acccgaaaat tgaagaagtg tttagcaaag cgtgggcttc aactcagtcg gcgccgaatc
       61 ggtcgaatca tgaaacgccg cggattgaca tctacctata cgatcgctca ttttaaaggg
      121 caacgaacag cttgtaatga agcgaaaaca gcgaatgtat tagatcggac ctttacacaa
      181 gaacagccat tggaagccat cgttacggat cttacttatg ttcgcgtggg gaaaaagtgg
      241 cattatatct gcttaatact tgatttgttt aatcgagaaa ttattggtta ttcctgtggt
      301 gagaagaaag atgcctcatt ggtaaaagaa gcctttggac ggataccgta ttctttaaca
      361 gacgt
//
LOCUS       DACCIQ010000280          358 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCIQ010000280 DACCIQ010000000
VERSION     DACCIQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.293"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>358
                     /locus_tag="IVM62_002931"
     CDS             <1..>358
                     /locus_tag="IVM62_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846806.1"
                     /translation="TCYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNY
                     ERTKKDVQYFKDTQTEEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNI
                     QNPTKKETALNYLKSV"
BASE COUNT          155 a           64 c           60 g           79 t
ORIGIN      
        1 ccacttgtta tgaggaaatg aacgatattt ttgtgaatca agaaaaacgt ttgacggata
       61 aaaacatgga aaaactcact cgtctttatc aaaacttgcc agaaaaacag caaaaagagg
      121 ttcaagacaa ctatgagcga accaaaaaag acgtgcaata ttttaaagat acgcagacag
      181 aagaagcttg ttataattta ttcagtgata caatcccttt ttcaactagt gaacaggaac
      241 gaaaagaacg gcaacaatca gttacctacg aacgttataa agcactgctt cagcaagcaa
      301 cgaacataca aaatccaacg aaaaaagaaa cggcattaaa ttatctaaaa agtgtcaa
//
LOCUS       DACCIQ010000281          323 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCIQ010000281 DACCIQ010000000
VERSION     DACCIQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.294"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            67..264
                     /locus_tag="IVM62_002932"
     CDS             67..264
                     /locus_tag="IVM62_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP6846807.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          121 a           31 c           66 g          105 t
ORIGIN      
        1 gttttttgaa atttaatgaa tgttagaata atagtctaaa aacattaatt taggggtgat
       61 tagattatga aacatttaaa aattttgtct attaaagaga cacaacttat ctatgggggt
      121 accactcata gtggaaaata ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg
      181 gtcgattggg ccaaggcaac tacttgtatt gcaggaatgt ctataggtgg ttttttaggt
      241 ggagcaattc cagggaagtg ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt
      301 atataatgat atatctataa gta
//
LOCUS       DACCIQ010000282          307 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCIQ010000282 DACCIQ010000000
VERSION     DACCIQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.295"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            complement(<1..302)
                     /locus_tag="IVM62_002933"
     CDS             complement(<1..302)
                     /locus_tag="IVM62_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP6846808.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DACCIQ010000283          299 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCIQ010000283 DACCIQ010000000
VERSION     DACCIQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.denovo.297"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..>299
                     /locus_tag="IVM62_002934"
     CDS             <1..>299
                     /locus_tag="IVM62_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6846809.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DACCIQ010000284        14771 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.059, whole genome shotgun sequence.
ACCESSION   DACCIQ010000284 DACCIQ010000000
VERSION     DACCIQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.059"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..2209
                     /locus_tag="IVM62_002935"
     CDS             <1..2209
                     /locus_tag="IVM62_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP6846810.1"
                     /translation="PAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAG
                     YDKTAFRIDCIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTG
                     IEGWEQDKAHRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLS
                     YVQECRPMLNQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAG
                     YEAPQQDTFSIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLE
                     DIYRTFNIDHPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTA
                     ELSTEQNENMIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRY
                     YPINEGAARRAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSL
                     LDTYARKLAENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGL
                     LDKIRSTGMGGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTE
                     RGIENLKADMASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDG
                     GTVEINRVANRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIK
                     CIQPLSGEKPSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            2210..3154
                     /locus_tag="IVM62_002936"
     CDS             2210..3154
                     /locus_tag="IVM62_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846811.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            3158..3361
                     /locus_tag="IVM62_002937"
     CDS             3158..3361
                     /locus_tag="IVM62_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846812.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(3661..3897)
                     /locus_tag="IVM62_002938"
     CDS             complement(3661..3897)
                     /locus_tag="IVM62_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846813.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(4080..4451)
                     /locus_tag="IVM62_002939"
     CDS             complement(4080..4451)
                     /locus_tag="IVM62_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846814.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(4448..5776)
                     /locus_tag="IVM62_002940"
     CDS             complement(4448..5776)
                     /locus_tag="IVM62_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6846815.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(5737..6066)
                     /locus_tag="IVM62_002941"
     CDS             complement(5737..6066)
                     /locus_tag="IVM62_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6846816.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(6324..6695)
                     /locus_tag="IVM62_002942"
     CDS             complement(6324..6695)
                     /locus_tag="IVM62_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6846817.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            6840..7025
                     /locus_tag="IVM62_002943"
     CDS             6840..7025
                     /locus_tag="IVM62_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6846818.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            7537..7755
                     /locus_tag="IVM62_002944"
     CDS             7537..7755
                     /locus_tag="IVM62_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846819.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            7968..8627
                     /locus_tag="IVM62_002945"
     CDS             7968..8627
                     /locus_tag="IVM62_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP6846820.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            8630..9973
                     /gene="vanS"
                     /locus_tag="IVM62_002946"
     CDS             8630..9973
                     /gene="vanS"
                     /locus_tag="IVM62_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6846821.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            10144..10950
                     /locus_tag="IVM62_002947"
     CDS             10144..10950
                     /locus_tag="IVM62_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP6846822.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            10968..11795
                     /gene="vanW"
                     /locus_tag="IVM62_002948"
     CDS             10968..11795
                     /gene="vanW"
                     /locus_tag="IVM62_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP6846823.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            11792..12763
                     /locus_tag="IVM62_002949"
     CDS             11792..12763
                     /locus_tag="IVM62_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP6846824.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            12756..13784
                     /gene="vanB"
                     /locus_tag="IVM62_002950"
     CDS             12756..13784
                     /gene="vanB"
                     /locus_tag="IVM62_002950"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP6846825.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            13790..14398
                     /locus_tag="IVM62_002951"
     CDS             13790..14398
                     /locus_tag="IVM62_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP6846826.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         3817 a         3528 c         3913 g         3513 t
ORIGIN      
        1 tccggcagcc gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg
       61 ccagcaaatg ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc
      121 ggcagaggca ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc
      181 ggacagctac gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat
      241 ccagagctac caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac
      301 aaccggcata gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt
      361 tgtcccctat atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg
      421 cttacagtcc ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc
      481 ttatgtgcag gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc
      541 gaaactggct gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat
      601 cgcgcaggag gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga
      661 agcaccccaa caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga
      721 ctatcggttt gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa
      781 ctatgacctt gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg
      841 cacattcaac atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt
      901 ggtggtgctg caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat
      961 ggaagtgccg gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca
     1021 gaacgaaaac atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga
     1081 acccgaacag ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc
     1141 cccggaggct gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc
     1201 ccgccgcgcc aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga
     1261 ataccggcac tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga
     1321 gccagagtat cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga
     1381 gaacatgaat aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc
     1441 gtccaatttc ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga
     1501 ggaatttcag tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat
     1561 cagcgccgac gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc
     1621 gtcgcaggag ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg
     1681 atgcccccat ctgaccgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg
     1741 gcactatgag aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg
     1801 ccggttaaag ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg
     1861 ggagtttgac ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt
     1921 tgaggggaag ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc
     1981 gcccaaggcc ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg
     2041 tatcaagtgc atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg
     2101 cagggagaaa gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc
     2161 caagcagcca ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt
     2221 tgaaaatgtg gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta
     2281 tcaaagtgac tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc
     2341 cgcagacaag accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg
     2401 tgatgttttc ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac
     2461 ccgtgacaag atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg
     2521 gggcaatctc tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca
     2581 gccggtgagt gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca
     2641 gcgttttgac ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc
     2701 ccatgacccg gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc
     2761 cgtccccggc gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga
     2821 ggcccggcgt attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac
     2881 gcatttcatg gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga
     2941 ccggctcttt gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca
     3001 tggcgtgtat gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg
     3061 gccctctatc cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc
     3121 ggccagaacc aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga
     3181 agaaaacctg atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac
     3241 cgccgtcatg ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa
     3301 gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata
     3361 acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt
     3421 tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct
     3481 tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg
     3541 taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt
     3601 ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt
     3661 ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg
     3721 ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt
     3781 ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc
     3841 accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct
     3901 gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga
     3961 aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag
     4021 gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt
     4081 tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg
     4141 cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac
     4201 gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac
     4261 agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa
     4321 atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa
     4381 tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt
     4441 ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca
     4501 cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat
     4561 acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg
     4621 ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg
     4681 tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca
     4741 cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc
     4801 ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca
     4861 ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct
     4921 ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg
     4981 ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc
     5041 tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca
     5101 atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg
     5161 gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct
     5221 gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct
     5281 gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc
     5341 cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg
     5401 aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt
     5461 ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg
     5521 caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt
     5581 gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa
     5641 attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag
     5701 ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta
     5761 tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta
     5821 caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg
     5881 tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat
     5941 gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc
     6001 aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt
     6061 tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg
     6121 gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc
     6181 ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa
     6241 atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct
     6301 aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca
     6361 attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt
     6421 ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta
     6481 aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg
     6541 ggaatggccc cgtcgttttc gatgttggca agatagcgcc agtcaatatt tattttctct
     6601 gccattgctt tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc cgcgccgaaa
     6661 gtttcaaagc cgggacaatc ttcaatattc gccatatcgc acctcccact tacattgtat
     6721 atttcatact ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt tatatttcac
     6781 attttgtttc ttttattctt cgagcaaaag aattataatg taccctgtgg aggtgtacta
     6841 tggactacat gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca cgatggataa
     6901 actactattg tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg atctggctca
     6961 ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag gcggttactg
     7021 aatagtataa tgaaaccggc gcattttgga tatggttttg aagagagaga aagcatggta
     7081 ttttgtcacg gaaaaactat aagatgatag atggcaggct gctccagacc gataaaaagt
     7141 attctcatct gaaacagaaa gaaaagattt acacatggat gtatgaagaa gcgaaacttt
     7201 atcatgacag aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc tgtatcagcc
     7261 gtatatggta gaattgaaaa ggccggcatc tggattcttt atggtgaagt ctccagacat
     7321 tggataactg ctggtctaat gcgcctttga gtatggggtg attgatttta ataccgggga
     7381 ggtacggctt tttgaggaaa atattctggg atttacattt ggcgattatt acatttcctg
     7441 taactgcaag gacacggatg atgaagatat gaggaatata gacttgctga ttttctactg
     7501 tcttgaacgg tattaggact gaatctgctg cgcggagtgc ggcaagagcg accggaggga
     7561 gtacacacag gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg caaaaaagag
     7621 gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa cgccgcaggc
     7681 gggcaagtgg tggatcagaa tatccgtaaa ggatattctg atccattgct tgccagtatt
     7741 atcgggatgt tttgacaacc gctgtaatcg aaacatccca aacattaaga aggtgttaat
     7801 tattattatc ttgatttaat tcgtttaaac caataatata gatacataca ccatgaaaag
     7861 aagcgagtat cccaatgacg gatgattaaa atccgaaaag aaatcgcttt gaaactacag
     7921 ggaaactaca gactgttatg ttatcttctt aaaatggagg gatttttatg tcgatacgaa
     7981 ttctacttgt cgaggatgat gatcatatct gtaatacagt aagggcgttt ctggctgagg
     8041 caggatatca ggtggatgcc tgcacagatg gaaatgaggc atacaccaag ttttacgaaa
     8101 acacttatca actggttatt cttgatatta tgctgcccgg tatgaacggg catgaacttt
     8161 tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat gatgacagcc ctgtcggatg
     8221 acgaaaacca aatccgggcg tttgatgcag aggcagacga ctatgtaaca aagccattca
     8281 agatgcagat tttactaaag cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa
     8341 aggaaatccg tgtcggcagg ctgacacttc tgccggagga tttcacggta ctttgtgacg
     8401 gtacagagct gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca
     8461 aaggcagaac cttaacgcat gaaatcattt tgtcacgtat atggggatat gactttgagg
     8521 gtgatggcag cacagtccac actcatatca aaaatttgcg ggccaagctg ccggaaaata
     8581 tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa tggaaagaaa
     8641 agggattttc attaaggttt tttcctatac gatcattgtc ctgctactgg ttgtcggtgt
     8701 aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg aattacagca
     8761 aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata ggcttgatat
     8821 tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt atattgaaga
     8881 taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata gttttagacc
     8941 cgactttctt tatgtggtac atagagatga taatatttcg attgttgctc aaaacaaggc
     9001 aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga ttgcgataat
     9061 ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa
     9121 agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga
     9181 gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg tcaggctgaa
     9241 agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg aggaaacaca
     9301 gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt
     9361 tctgttggag ggaatgcttg gaaatatcgg tgactacaaa gatcattcta agtatctgcg
     9421 cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac tggagcttgt
     9481 cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag ggcgcacggt
     9541 tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac
     9601 agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc
     9661 caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac ggatatggag
     9721 tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc acattgatga
     9781 tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa
     9841 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
     9901 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
     9961 atcaacattg taaatattta aaacttaaat gattttgacc gacaggtata accctgccgg
    10021 tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt
    10081 gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat
    10141 caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa
    10201 tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg
    10261 tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca
    10321 actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc
    10381 gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc
    10441 atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt
    10501 cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg
    10561 attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt
    10621 gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg
    10681 gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac
    10741 ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt
    10801 ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg
    10861 tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc
    10921 cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa
    10981 gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct
    11041 tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg gaaaaatcgc
    11101 tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc ggatttgaca
    11161 tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc ttaaacggct
    11221 tattgataag accgggggaa accttttctt tctggtggct ggtacgccat gcggacaaag
    11281 atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc atgtcgggcg
    11341 gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg ccattgacaa
    11401 ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt gacgagatta
    11461 aaggggtgga tgcaaccatt ccagagggct ggattgattt aaaagtgcga aacgataccg
    11521 actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt caggtgtccg
    11581 ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg
    11641 aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg
    11701 gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt tatcccctcc
    11761 cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca ttactgtttt
    11821 tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt ttcatattat
    11881 ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg ctggcaatca
    11941 atgcgttagc gtaggccata agtccgaggt ttccgaggcg acaattcttg cgctgagaaa
    12001 ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg atacgactgc
    12061 cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca gcgttgcgga
    12121 ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca tgcacgccgt
    12181 ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg atatgactgt
    12241 gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc ggggatttgg
    12301 atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg tccagcttga
    12361 tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg cggatacccg
    12421 ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt taatcaacac
    12481 cgcgcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa gcggaaagct
    12541 gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata ccgattgctc
    12601 gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga atgtgatcat
    12661 cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag aaaaaacaat
    12721 caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa gtcgcaatca
    12781 tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa atccgcaata gaaattgctg
    12841 cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa aacggtgtat
    12901 ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc gccatactct
    12961 ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac gaaacacggc
    13021 gtattgatgt ggctttcccg gttttgcatg gcaaattcgg ggaggatggt gcgatacagg
    13081 ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc tccgcagctt
    13141 gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc gttcccgaat
    13201 ttcaaattat tgataaaggt gacaagccgg aggcgggtgc gcttacctac cctgtctttg
    13261 tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac
    13321 ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt gagcaagcga
    13381 tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg attgtcggcg
    13441 aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa aacgagccgg
    13501 aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc gaggaacgaa
    13561 atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga gggcttgccc
    13621 gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc aataccatgc
    13681 ccggttttac atcgtacagc cgctacccac gtatggtggc cgccgcagga atcacgcttc
    13741 ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta tggaaaatgg
    13801 ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt acgccacatg
    13861 ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa
    13921 ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg gctatggctt
    13981 gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc gttgggcagc
    14041 gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc gagccgagtt
    14101 gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg caattgatct
    14161 tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact tcgattttat
    14221 ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac aaaatcgaag
    14281 atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg aatggtggca
    14341 ctataagttg attgatgagc cataccccga tacctatttt aattttgccg tttcataatg
    14401 aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt catggacaat
    14461 cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat agtattcaat
    14521 ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca cgcccatttt
    14581 gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag
    14641 gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg ctctaatcca
    14701 cttccaccac tcaaaaaagc cttgcccctc catgaactga accccaaaag ttgaactatt
    14761 taatggactg t
//
LOCUS       DACCIQ010000285        14771 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.060, whole genome shotgun sequence.
ACCESSION   DACCIQ010000285 DACCIQ010000000
VERSION     DACCIQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.060"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..2209
                     /locus_tag="IVM62_002952"
     CDS             <1..2209
                     /locus_tag="IVM62_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP6846827.1"
                     /translation="PAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAG
                     YDKTAFRIDCIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTG
                     IEGWEQDKAHRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLS
                     YVQECRPMLNQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAG
                     YEAPQQDTFSIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLE
                     DIYRTFNIDHPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTA
                     ELSTEQNENMIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRY
                     YPINEGAARRAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSL
                     LDTYARKLAENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGL
                     LDKIRSTGMGGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTE
                     RGIENLKADMASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDG
                     GTVEINRVANRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIK
                     CIQPLSGEKPSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            2210..3154
                     /locus_tag="IVM62_002953"
     CDS             2210..3154
                     /locus_tag="IVM62_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846828.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            3158..3361
                     /locus_tag="IVM62_002954"
     CDS             3158..3361
                     /locus_tag="IVM62_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846829.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(3661..3897)
                     /locus_tag="IVM62_002955"
     CDS             complement(3661..3897)
                     /locus_tag="IVM62_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6846830.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(4080..4451)
                     /locus_tag="IVM62_002956"
     CDS             complement(4080..4451)
                     /locus_tag="IVM62_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846831.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(4448..5776)
                     /locus_tag="IVM62_002957"
     CDS             complement(4448..5776)
                     /locus_tag="IVM62_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6846832.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(5737..6066)
                     /locus_tag="IVM62_002958"
     CDS             complement(5737..6066)
                     /locus_tag="IVM62_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6846833.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(6324..6695)
                     /locus_tag="IVM62_002959"
     CDS             complement(6324..6695)
                     /locus_tag="IVM62_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6846834.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            6840..7025
                     /locus_tag="IVM62_002960"
     CDS             6840..7025
                     /locus_tag="IVM62_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6846835.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            7537..7755
                     /locus_tag="IVM62_002961"
     CDS             7537..7755
                     /locus_tag="IVM62_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846836.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            7968..8627
                     /gene="vanR"
                     /locus_tag="IVM62_002962"
     CDS             7968..8627
                     /gene="vanR"
                     /locus_tag="IVM62_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6846837.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFDGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            8630..9973
                     /gene="vanS"
                     /locus_tag="IVM62_002963"
     CDS             8630..9973
                     /gene="vanS"
                     /locus_tag="IVM62_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6846838.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            10144..10950
                     /locus_tag="IVM62_002964"
     CDS             10144..10950
                     /locus_tag="IVM62_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP6846839.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            10968..11795
                     /gene="vanW"
                     /locus_tag="IVM62_002965"
     CDS             10968..11795
                     /gene="vanW"
                     /locus_tag="IVM62_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP6846840.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            11792..12763
                     /locus_tag="IVM62_002966"
     CDS             11792..12763
                     /locus_tag="IVM62_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP6846841.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            12756..13784
                     /gene="vanB"
                     /locus_tag="IVM62_002967"
     CDS             12756..13784
                     /gene="vanB"
                     /locus_tag="IVM62_002967"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP6846842.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            13790..14398
                     /locus_tag="IVM62_002968"
     CDS             13790..14398
                     /locus_tag="IVM62_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP6846843.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         3817 a         3528 c         3912 g         3514 t
ORIGIN      
        1 tccggcagcc gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg
       61 ccagcaaatg ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc
      121 ggcagaggca ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc
      181 ggacagctac gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat
      241 ccagagctac caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac
      301 aaccggcata gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt
      361 tgtcccctat atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg
      421 cttacagtcc ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc
      481 ttatgtgcag gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc
      541 gaaactggct gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat
      601 cgcgcaggag gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga
      661 agcaccccaa caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga
      721 ctatcggttt gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa
      781 ctatgacctt gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg
      841 cacattcaac atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt
      901 ggtggtgctg caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat
      961 ggaagtgccg gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca
     1021 gaacgaaaac atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga
     1081 acccgaacag ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc
     1141 cccggaggct gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc
     1201 ccgccgcgcc aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga
     1261 ataccggcac tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga
     1321 gccagagtat cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga
     1381 gaacatgaat aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc
     1441 gtccaatttc ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga
     1501 ggaatttcag tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat
     1561 cagcgccgac gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc
     1621 gtcgcaggag ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg
     1681 atgcccccat ctgaccgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg
     1741 gcactatgag aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg
     1801 ccggttaaag ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg
     1861 ggagtttgac ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt
     1921 tgaggggaag ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc
     1981 gcccaaggcc ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg
     2041 tatcaagtgc atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg
     2101 cagggagaaa gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc
     2161 caagcagcca ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt
     2221 tgaaaatgtg gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta
     2281 tcaaagtgac tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc
     2341 cgcagacaag accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg
     2401 tgatgttttc ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac
     2461 ccgtgacaag atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg
     2521 gggcaatctc tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca
     2581 gccggtgagt gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca
     2641 gcgttttgac ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc
     2701 ccatgacccg gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc
     2761 cgtccccggc gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga
     2821 ggcccggcgt attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac
     2881 gcatttcatg gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga
     2941 ccggctcttt gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca
     3001 tggcgtgtat gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg
     3061 gccctctatc cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc
     3121 ggccagaacc aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga
     3181 agaaaacctg atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac
     3241 cgccgtcatg ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa
     3301 gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata
     3361 acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt
     3421 tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct
     3481 tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg
     3541 taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt
     3601 ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt
     3661 ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg
     3721 ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt
     3781 ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc
     3841 accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct
     3901 gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga
     3961 aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag
     4021 gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt
     4081 tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg
     4141 cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac
     4201 gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac
     4261 agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa
     4321 atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa
     4381 tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt
     4441 ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca
     4501 cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat
     4561 acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg
     4621 ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg
     4681 tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca
     4741 cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc
     4801 ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca
     4861 ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct
     4921 ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg
     4981 ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc
     5041 tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca
     5101 atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg
     5161 gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct
     5221 gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct
     5281 gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc
     5341 cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg
     5401 aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt
     5461 ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg
     5521 caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt
     5581 gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa
     5641 attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag
     5701 ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta
     5761 tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta
     5821 caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg
     5881 tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat
     5941 gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc
     6001 aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt
     6061 tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg
     6121 gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc
     6181 ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa
     6241 atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct
     6301 aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca
     6361 attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt
     6421 ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta
     6481 aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg
     6541 ggaatggccc cgtcgttttc gatgttggca agatagcgcc agtcaatatt tattttctct
     6601 gccattgctt tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc cgcgccgaaa
     6661 gtttcaaagc cgggacaatc ttcaatattc gccatatcgc acctcccact tacattgtat
     6721 atttcatact ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt tatatttcac
     6781 attttgtttc ttttattctt cgagcaaaag aattataatg taccctgtgg aggtgtacta
     6841 tggactacat gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca cgatggataa
     6901 actactattg tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg atctggctca
     6961 ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag gcggttactg
     7021 aatagtataa tgaaaccggc gcattttgga tatggttttg aagagagaga aagcatggta
     7081 ttttgtcacg gaaaaactat aagatgatag atggcaggct gctccagacc gataaaaagt
     7141 attctcatct gaaacagaaa gaaaagattt acacatggat gtatgaagaa gcgaaacttt
     7201 atcatgacag aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc tgtatcagcc
     7261 gtatatggta gaattgaaaa ggccggcatc tggattcttt atggtgaagt ctccagacat
     7321 tggataactg ctggtctaat gcgcctttga gtatggggtg attgatttta ataccgggga
     7381 ggtacggctt tttgaggaaa atattctggg atttacattt ggcgattatt acatttcctg
     7441 taactgcaag gacacggatg atgaagatat gaggaatata gacttgctga ttttctactg
     7501 tcttgaacgg tattaggact gaatctgctg cgcggagtgc ggcaagagcg accggaggga
     7561 gtacacacag gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg caaaaaagag
     7621 gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa cgccgcaggc
     7681 gggcaagtgg tggatcagaa tatccgtaaa ggatattctg atccattgct tgccagtatt
     7741 atcgggatgt tttgacaacc gctgtaatcg aaacatccca aacattaaga aggtgttaat
     7801 tattattatc ttgatttaat tcgtttaaac caataatata gatacataca ccatgaaaag
     7861 aagcgagtat cccaatgacg gatgattaaa atccgaaaag aaatcgcttt gaaactacag
     7921 ggaaactaca gactgttatg ttatcttctt aaaatggagg gatttttatg tcgatacgaa
     7981 ttctacttgt cgaggatgat gatcatatct gtaatacagt aagggcgttt ctggctgagg
     8041 caggatatca ggtggatgcc tgcacagatg gaaatgaggc atacaccaag ttttacgaaa
     8101 acacttatca actggttatt cttgatatta tgctgcccgg tatgaacggg catgaacttt
     8161 tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat gatgacagcc ctgtcggatg
     8221 acgaaaacca aatccgggcg tttgatgcag aggcagacga ctatgtaaca aagccattca
     8281 agatgcagat tttactaaag cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa
     8341 aggaaatccg tgtcggcagg ctgacacttc tgccggagga tttcacggta ctttgtgacg
     8401 gtacagagct gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca
     8461 aaggcagaac cttaacgcat gaaatcattt tgtcacgtat atggggatat gactttgatg
     8521 gtgatggcag cacagtccac actcatatca aaaatttgcg ggccaagctg ccggaaaata
     8581 tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa tggaaagaaa
     8641 agggattttc attaaggttt tttcctatac gatcattgtc ctgctactgg ttgtcggtgt
     8701 aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg aattacagca
     8761 aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata ggcttgatat
     8821 tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt atattgaaga
     8881 taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata gttttagacc
     8941 cgactttctt tatgtggtac atagagatga taatatttcg attgttgctc aaaacaaggc
     9001 aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga ttgcgataat
     9061 ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa
     9121 agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga
     9181 gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg tcaggctgaa
     9241 agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg aggaaacaca
     9301 gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt
     9361 tctgttggag ggaatgcttg gaaatatcgg tgactacaaa gatcattcta agtatctgcg
     9421 cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac tggagcttgt
     9481 cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag ggcgcacggt
     9541 tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac
     9601 agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc
     9661 caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac ggatatggag
     9721 tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc acattgatga
     9781 tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa
     9841 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
     9901 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
     9961 atcaacattg taaatattta aaacttaaat gattttgacc gacaggtata accctgccgg
    10021 tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt
    10081 gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat
    10141 caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa
    10201 tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg
    10261 tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca
    10321 actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc
    10381 gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc
    10441 atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt
    10501 cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg
    10561 attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt
    10621 gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg
    10681 gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac
    10741 ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt
    10801 ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg
    10861 tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc
    10921 cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa
    10981 gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct
    11041 tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg gaaaaatcgc
    11101 tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc ggatttgaca
    11161 tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc ttaaacggct
    11221 tattgataag accgggggaa accttttctt tctggtggct ggtacgccat gcggacaaag
    11281 atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc atgtcgggcg
    11341 gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg ccattgacaa
    11401 ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt gacgagatta
    11461 aaggggtgga tgcaaccatt ccagagggct ggattgattt aaaagtgcga aacgataccg
    11521 actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt caggtgtccg
    11581 ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg
    11641 aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg
    11701 gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt tatcccctcc
    11761 cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca ttactgtttt
    11821 tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt ttcatattat
    11881 ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg ctggcaatca
    11941 atgcgttagc gtaggccata agtccgaggt ttccgaggcg acaattcttg cgctgagaaa
    12001 ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg atacgactgc
    12061 cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca gcgttgcgga
    12121 ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca tgcacgccgt
    12181 ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg atatgactgt
    12241 gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc ggggatttgg
    12301 atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg tccagcttga
    12361 tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg cggatacccg
    12421 ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt taatcaacac
    12481 cgcgcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa gcggaaagct
    12541 gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata ccgattgctc
    12601 gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga atgtgatcat
    12661 cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag aaaaaacaat
    12721 caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa gtcgcaatca
    12781 tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa atccgcaata gaaattgctg
    12841 cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa aacggtgtat
    12901 ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc gccatactct
    12961 ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac gaaacacggc
    13021 gtattgatgt ggctttcccg gttttgcatg gcaaattcgg ggaggatggt gcgatacagg
    13081 ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc tccgcagctt
    13141 gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc gttcccgaat
    13201 ttcaaattat tgataaaggt gacaagccgg aggcgggtgc gcttacctac cctgtctttg
    13261 tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac
    13321 ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt gagcaagcga
    13381 tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg attgtcggcg
    13441 aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa aacgagccgg
    13501 aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc gaggaacgaa
    13561 atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga gggcttgccc
    13621 gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc aataccatgc
    13681 ccggttttac atcgtacagc cgctacccac gtatggtggc cgccgcagga atcacgcttc
    13741 ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta tggaaaatgg
    13801 ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt acgccacatg
    13861 ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa
    13921 ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg gctatggctt
    13981 gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc gttgggcagc
    14041 gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc gagccgagtt
    14101 gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg caattgatct
    14161 tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact tcgattttat
    14221 ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac aaaatcgaag
    14281 atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg aatggtggca
    14341 ctataagttg attgatgagc cataccccga tacctatttt aattttgccg tttcataatg
    14401 aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt catggacaat
    14461 cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat agtattcaat
    14521 ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca cgcccatttt
    14581 gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag
    14641 gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg ctctaatcca
    14701 cttccaccac tcaaaaaagc cttgcccctc catgaactga accccaaaag ttgaactatt
    14761 taatggactg t
//
LOCUS       DACCIQ010000286         7810 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.108, whole genome shotgun sequence.
ACCESSION   DACCIQ010000286 DACCIQ010000000
VERSION     DACCIQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.108"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            81..650
                     /locus_tag="IVM62_002969"
     CDS             81..650
                     /locus_tag="IVM62_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6846844.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            717..1454
                     /locus_tag="IVM62_002970"
     CDS             717..1454
                     /locus_tag="IVM62_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6846845.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1685..2953
                     /locus_tag="IVM62_002971"
     CDS             1685..2953
                     /locus_tag="IVM62_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6846846.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3440..3904
                     /locus_tag="IVM62_002972"
     CDS             3440..3904
                     /locus_tag="IVM62_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6846847.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3888..4517
                     /locus_tag="IVM62_002973"
     CDS             3888..4517
                     /locus_tag="IVM62_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6846848.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            4871..6349
                     /gene="msr"
                     /locus_tag="IVM62_002974"
     CDS             4871..6349
                     /gene="msr"
                     /locus_tag="IVM62_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6846849.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6396..6842
                     /locus_tag="IVM62_002975"
     CDS             6396..6842
                     /locus_tag="IVM62_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6846850.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7041..7556)
                     /locus_tag="IVM62_002976"
     CDS             complement(7041..7556)
                     /locus_tag="IVM62_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6846851.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7673..>7810
                     /locus_tag="IVM62_002977"
     CDS             7673..>7810
                     /locus_tag="IVM62_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6846852.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LK"
BASE COUNT         2609 a         1365 c         1597 g         2239 t
ORIGIN      
        1 aggaatagtt aagtatgttt tataagtatt agacatcata agccgtccgt tataaagtaa
       61 ggataaaagg agggagcaaa atgacaagcc tcatcgaaaa acaaatcgtc aataaagaga
      121 ttcgagtaac ggatccgctc tttgaagtga tccatcagat ccaagcggaa aacgaagagc
      181 ctttagcagc gctgaatact gggtaccatg aaccagcaga tatccgcaaa cggctagaaa
      241 atatcattag tgacaaagta gatgatacgg tgactgttct gctgcctttt tatacagatt
      301 tcggcaaaca tattagtata gggaaaaatg tctttatcaa tcgtcaagtg atgttcgttg
      361 acttaggtgg catctgttta gaagattcag tattgattgg tcctcgtgtc aacctcatca
      421 cggtgaacca tctaaccgat ccaaaagaac gaagaggctt gtcagtaaag ccaatccaca
      481 tcaagaaaaa tgcttggatc ggagcaggag caactattct gccaggcgta acgattggcg
      541 agaatgccat tgttgcagcg aatgcaactg ttacgaaaga tgtcccagac aataccatcg
      601 ttgcaggaat ccctgccaaa atcgtcaaac ctgtcgaacg gattatttga acagtaagat
      661 gaaataagag atagtgagta taagcaagag atttttcaaa acaggaggag aatagaatga
      721 atgatttatc agaaaaagtg attgtcatca tgggcgcttc aagcggaatc ggtgaagcaa
      781 caacgaagct attagcagaa aaaggggcta aactagttat agctgcacgc cgagaagatc
      841 gcctaaaagc aataaaagaa tctttaccag aagctgagct atatattcaa acagcagatg
      901 taagagattt tgcacaagtc caagcagtta tcgatttagc gatggaaaag ttcggtcgaa
      961 tcgatgtatt gtacaataat gcaggaatca tgccaaccgc tccattagtt gaaggtcacc
     1021 gtgatgaatg gcaaaatatg cttgatatca acatcatggg tgtgttaaat ggaatttccg
     1081 ccgtacttcc aatcatggaa aaacaaaaat ctgggcatat catttcaact gattctgtgg
     1141 cgggacatgt tgtttatcct gattccgccg tttattgcgg aacaaaattt gctgtccgtg
     1201 caatcatgga aggattacgt caagagcaac ggcaaaataa catcaaatct acgatcatct
     1261 ctccaggagc tgttcaaact gaattatatc aaaccatcag cgacaaaaag gctgctcttg
     1321 aacttcatga agcgcaaaaa gaatggggat taacatctga agatatagct tctgctgtag
     1381 catttgcgat agaaacgcca gaccggatgt ctgtcagcaa catgattatc cgtccgacga
     1441 cacaggaagt ataatccata acggataata tccagagaga attacttgtt aaatagttat
     1501 tcaaacgacc atcaatttaa ggcgtgaatg gagaagaata ttcttcattt acgtcttttt
     1561 atgcagaaaa agcaaattta ttaaaaagaa acaaatgtta tttaaatatg gacattgact
     1621 tgcttttact tttttagtta cactgttatt taggaaaagt ttcataattc aaaggagaaa
     1681 aaagatgaaa gcaaagaatt tattatttag cgttattctt ttaggaaatc tagcagttgc
     1741 caacacaaca gtgctagctg aagaatcgac agaaactgtc gcaactcaga catcgagttc
     1801 ttcagaaaca ggaattgaaa acgatacaga aagtactttg cagtcagcta ctgtctcaga
     1861 aaatacaatt tcaaccgaag aaccaacaac tgaaacaaca gaacaaacac aaaccgaaga
     1921 cagtacatca gcagaagctc agaacaatcc agagaatgca gaagaaatca cgaaccaatt
     1981 gacagatggg caaacattga cacataccca tcaagcagat ttgtctgaat tcgaaaacta
     2041 tactggctat gctttttatg ctcgctcatc tgctaattct caacaagcgt tcattgatag
     2101 tattgcctca actgcacaaa gtttggcaag tgccaatgac ttatatgcat cagtcatgat
     2161 tgctcaagcc atcgttgaaa gtggctgggg gaacagtact ttggcttctg cccctaatta
     2221 caatctattt gggattaaag gaagctataa tggccaatct gttacgatgc ctacttcgga
     2281 atatgtgaat gggcaatgga ttacggtgaa tgccgctttt agaaaatatc cttcgtacaa
     2341 agaatcattg caagataacg taacggtatt gaagacgact tcttttcagc caggcgttta
     2401 ctactattcc ggtgcatgga aaagcaatac gaactcttac aaagatgcta cggcttggct
     2461 aacaggtcgt tatgccactg cgcctaatta tggttctaca ttgaacaatg taatcgaaac
     2521 ctataatctg acacagtatg atacagcacc aacttcaact tctccgatgt atcgtctgta
     2581 taaccgtcat acaggagaac atttatatac attgaatgcc ggagaaaaaa attatctgcc
     2641 aactgtaggc tgggaatatg aagggattgc ttggcaagca ccaaacagcg gacaacctgt
     2701 ctaccgttta tacaatcctt acagtggaga ccatcattac acaatggcac aatctgagat
     2761 caactcttta acaaaaatcg gttggcgtta tgaaggtcta agtttttatt ctggtggttc
     2821 caagccaatt tatcgactat ttaatccaaa tgaaaaaact ggtacccatc actatacctt
     2881 gagtgctaag gaaagagatt ggctaacacc gatgggctgg agatatgaag gaataggatt
     2941 ttatggttat taaaaagagg tagtgaaaaa gctgccctgc atcaagtagt aagaacagcc
     3001 aaacaaaaac agattttcat gttttttgtt tggttgaact tattaccgca gatgcaagtt
     3061 tttgaacacc gtttactcaa aaaaggttgt gacaacatct tgtcataact tttttattac
     3121 tgtttaagca tactgatcca atttctaata ttcgacggga ttcttgcaaa aggcgatttc
     3181 ggaaattttt ggcggtgtaa aattatctac tgcagaagag ctatcttcta tagtttgata
     3241 agaatcaata atcttggttt tagcgttgtg ctttctttat cattttttcc gttcattcta
     3301 caaaaaattt cttttctcta tacttttcta ttgagagcgt tttttttatt acacagctga
     3361 ttctttgact tttttctttt tttattctat tattgacaag tcaatcgttg acgagtcaat
     3421 tattagaaag gaggaaatta tgtctttaga agagattagt caccttttat atcaaatcaa
     3481 attagccgat caaaaaatcg tccagatctt tgaagaaaat atcggtataa gtcttactag
     3541 atacgagatc atgatgagac tgaaagaaga aaatccattg ctacaaagca gtttgcaaga
     3601 aaagttgcag attgatcaag gagcagttac tcgtcatctg aaggtattgg aagcaaatgg
     3661 ctatgtttcg cgtcagagaa atcctcagaa caaccgggaa gtgtatgtag cgctaacaga
     3721 aaaagcccag aagaaaatca agcattgtga aatggaccat aaagatacag gttccgtgct
     3781 atccccggct ttcagcgatg tagaaatgaa acaatttcta acacttttag ataaattgaa
     3841 tacccaactt tgcaatataa caacagaaac gaaagaggtt aataataatg gaacaaaaaa
     3901 atgatttttc aacaattatg actggtagaa gatccgttcg cgtatatgat gaaacataca
     3961 aaatcccaca cgaagaaatg ctggaaatga tcaaagaagc gacgactgca ccttcttctg
     4021 tcaatatgaa accatggcgc tttgtggtcg ctgaaagcga ggaagcaaaa gaaatcttgc
     4081 gtcctttgat ccgtttcaat acgagacaaa atgatacttc ttctgcaatg gtcatgattt
     4141 ttggcgatat gcaatgttac gaatatggcg aagaaatcta caaccaagct tacgaatcag
     4201 ggaagatgcc aaaagaagtc agagatcaac aattagctgc gatcattcct tattacaaga
     4261 gtttttcaag agaagaaatg aacgatgtag tcaaagtcga ttctagctta gcggctatgc
     4321 aattcatgtt agttgcgcgt gatcatggct atgaaacaaa tccaattggt ggattcgaag
     4381 cagatcagtt ggcagaggca tttggtttgg ataaagaccg ttatgttcca gttattattt
     4441 tatcaatagg aaaagcggta gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga
     4501 tcactatgtt cgaataaaag gttttcagtt actattcagt agctattgct ttttttgaat
     4561 cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg actgcatcga tgaaattacg
     4621 tttcgaactt ttgaataaca actaaataaa ttcaaaagac atgtataaac aacaatttca
     4681 tggatggagt atgtccattt tttcgaacga cagtagaata ggtgaaagct atgacagatt
     4741 tggtgattta gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc agtttttttg
     4801 caaacagatt tttctgctct tttttagtat cgataattgt tgttttacat gaaataggag
     4861 tgtaaaacat atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa
     4921 ccagctagaa ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat
     4981 aggagaaaat ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc
     5041 cgatcatggg aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct
     5101 cgctgaagca aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga
     5161 aacttcagag cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct
     5221 ttcttcttat gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag
     5281 tattgatcga ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca
     5341 tgatcgctat tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat
     5401 ccgagagttt tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac
     5461 tcagctacga gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca
     5521 agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca
     5581 aatcagactg gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca
     5641 aaagaatgcg aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca
     5701 gcagatgaaa caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc
     5761 catcatggga caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga
     5821 ttttcagttt tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac
     5881 aaccttatta gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag
     5941 ctttcaagta tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt
     6001 tgtcatgaga caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg
     6061 gtttgctcag gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg
     6121 tctgacgatt gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac
     6181 taattttatt gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg
     6241 aacgattttg tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta
     6301 tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga
     6361 tgattttcgt gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa
     6421 taatttttac aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat
     6481 ggctgcatca agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg
     6541 tggctttcat tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac
     6601 taccagtaaa tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt
     6661 taggaatggt gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc
     6721 aattattctc tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc
     6781 agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat
     6841 gatggaacac tgttatttga aaaaaggcct tggcatgctt tttgtcatag tctcttgaat
     6901 gcagttctag aaaaatagtg atccgaacga taggttttgg cctgtcgttc ggatcgcttt
     6961 ttcttttcca actgtattgc tcttttattg aagataattt gctcttcaat agatatttct
     7021 agaaaggttc tgtttatccc ttactcaaaa aactgctgca tttgttctct tacttgctga
     7081 caccattgga gttcctgctg acaaagcata attctgcgct ggatcacaac ataacgtcca
     7141 aattcttgtt tcttgtctag cgagaggtca ttcaattttt tttgattttt ttgcatgatt
     7201 ttgttcagct gttgttctct agtgaatagc agtccagtag ctgttgtttt atcgagcgtc
     7261 gaaaaagcat agatttttgc tagaaattca tctttttgcg taggagcggg agtttcctct
     7321 ttgatccatt cctctacaag agcacgtcct tcatcagtca agtggtagat ctttttttga
     7381 gagtgctcgt ctttgtccaa aacggtcaca taaccctgct ggtctaactt ggcaagcgtt
     7441 gtatagatct gactgtgatg ggcttcccaa aaaattgtga tcagctgctt caattcatac
     7501 ccagaacgtg gtttggataa aagcacgcat aataatatat aggctaaagt attcataaaa
     7561 atcctacctc tcgtcttatt ttctttatta tatcacagta aagtttccac gttttacatg
     7621 tgcatattga catgtaaaat aaatcatgta tgatgctaat agagagaaag ggatggaaat
     7681 attggagaat caaatagata tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc
     7741 agcgctagtt gtagcagcta cctgtatgat cgcttttatg ggagtgggtc tagtagaccc
     7801 aatcttaaaa
//
LOCUS       DACCIQ010000287         5852 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.121, whole genome shotgun sequence.
ACCESSION   DACCIQ010000287 DACCIQ010000000
VERSION     DACCIQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.121"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            90..986
                     /locus_tag="IVM62_002978"
     CDS             90..986
                     /locus_tag="IVM62_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6846853.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1136..2077)
                     /locus_tag="IVM62_002979"
     CDS             complement(1136..2077)
                     /locus_tag="IVM62_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6846854.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2203..3324
                     /locus_tag="IVM62_002980"
     CDS             2203..3324
                     /locus_tag="IVM62_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6846855.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3582..5084
                     /locus_tag="IVM62_002981"
     CDS             3582..5084
                     /locus_tag="IVM62_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6846856.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5151..5678
                     /locus_tag="IVM62_002982"
     CDS             5151..5678
                     /locus_tag="IVM62_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6846857.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1918 a         1031 c         1226 g         1677 t
ORIGIN      
        1 catacaaata taggaaacgt tttactggct ttttactatt taaacaggct atcataggaa
       61 aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa
      121 aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag
      181 atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga
      241 tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc ccatttacca
      301 atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga
      361 agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa
      421 aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat ttttcttcta
      481 gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag
      541 aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac
      601 atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg
      661 aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat
      721 ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc
      781 atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg
      841 aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag
      901 aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg
      961 acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa
     1021 caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg
     1081 ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt
     1141 ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg
     1201 tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag
     1261 tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt
     1321 caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt
     1381 cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt
     1441 tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc
     1501 catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta
     1561 aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca
     1621 agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac
     1681 cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta
     1741 catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg
     1801 attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt
     1861 ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca
     1921 cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag
     1981 gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc
     2041 tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat
     2101 gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa
     2161 tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca
     2221 attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc
     2281 tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact
     2341 gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc
     2401 ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca
     2461 gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact
     2521 atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa
     2581 gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta
     2641 ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact
     2701 aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct
     2761 gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa
     2821 aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag
     2881 caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc
     2941 ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc
     3001 agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa
     3061 gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca
     3121 aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga
     3181 ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa
     3241 gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga
     3301 tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc
     3361 aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg
     3421 cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg
     3481 gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt
     3541 tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa
     3601 aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa
     3661 attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt
     3721 tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat
     3781 ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg
     3841 atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc
     3901 ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac
     3961 tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt
     4021 tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca
     4081 tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg
     4141 aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac
     4201 ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa
     4261 agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga
     4321 aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg
     4381 atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca
     4441 gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc
     4501 aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc
     4561 tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc
     4621 ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg
     4681 ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg
     4741 aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat
     4801 ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc
     4861 agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg
     4921 aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag
     4981 aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc
     5041 tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag
     5101 aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg
     5161 taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg
     5221 tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt
     5281 tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac
     5341 aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg
     5401 attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag
     5461 cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc
     5521 ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg
     5581 ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat
     5641 tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa
     5701 taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct
     5761 tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga
     5821 aattttgttt ctttagatat atactctagt ca
//
LOCUS       DACCIQ010000288         4379 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.140, whole genome shotgun sequence.
ACCESSION   DACCIQ010000288 DACCIQ010000000
VERSION     DACCIQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.140"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..1392
                     /locus_tag="IVM62_002983"
     CDS             <1..1392
                     /locus_tag="IVM62_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6846858.1"
                     /translation="TLFDSRFKTLQQNLSGKSLEALELEQEEIRRFQNCSLYPVYHGS
                     AKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLR
                     DSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQR
                     ERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKV
                     QMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPL
                     PLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPV
                     STPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDT
                     QLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNS
                     RIDKVRYMFNKIT"
     gene            1502..1552
                     /locus_tag="IVM62_002984"
                     /pseudo
     CDS             1502..1552
                     /locus_tag="IVM62_002984"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            1586..2962
                     /gene="tet(L)"
                     /locus_tag="IVM62_002985"
     CDS             1586..2962
                     /gene="tet(L)"
                     /locus_tag="IVM62_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6846859.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            3526..>4379
                     /locus_tag="IVM62_002986"
     CDS             3526..>4379
                     /locus_tag="IVM62_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP6846860.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNWFCC
                     KVLNLLSN"
BASE COUNT         1436 a          668 c          893 g         1382 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tgtccggtaa atcattagaa
       61 gcattagaac tcgaacaaga ggaaatcaga agatttcaga attgctcctt gtaccctgtt
      121 tatcatggaa gcgcaaaaag caacataggg attgagcagc ttatagaagt gataacgaat
      181 aaattttatt catcaacata cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt
      241 gaatattcgg aagaaagaca acgtcttgca tatgtacgcc tttatggcgg aatcctgcat
      301 ttgcgggatt cggttagaat atcggaaaag gaaaaaataa aaattacaga aatgtatact
      361 tcaataaatg gtgaattatg taaaattgat aaggcttatt ccggggaaat tgttattttg
      421 caaaatgagt ttttgaagct aaatagtgtt cttggagata caaagctatt gccacagaga
      481 gagagaattg aaaatccgct ccctctgctg caaacaactg ttgaaccgag caaacctcaa
      541 caaagggaaa tgttacttga tgcactttta gaaatctccg acagtgaccc gcttctacaa
      601 tattatgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
      661 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
      721 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
      781 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
      841 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
      901 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
      961 tgtaaaatct gttttaagta tggcttatac tatagccctg ttagtacccc agcagatttt
     1021 cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac agaattgtta
     1081 gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg agcatacaac
     1141 gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa tgaggtcatt
     1201 cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt aactttcttt
     1261 acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac taccggtgaa
     1321 cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata tatgttcaat
     1381 aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta aataagatga
     1441 aatatttttt aataaagatt tgaattaaag tgtaaaggag gaattaatta tgaagtgaaa
     1501 tgaatgtaac agggttcaat taaaagaggg aagcgtatca ttaaccctat aaactacgtc
     1561 tgccctcatt attggagggt gaaatgtgaa tacatcctat tcacaatcga atttacgaca
     1621 caaccaaatt ttaatttggc tttgcatttt atcttttttt agcgtattaa atgaaatggt
     1681 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
     1741 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
     1801 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
     1861 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
     1921 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
     1981 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
     2041 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     2101 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     2161 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     2221 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     2281 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     2341 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     2401 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     2461 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     2521 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     2581 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     2641 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     2701 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     2761 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     2821 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     2881 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     2941 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     3001 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     3061 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     3121 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     3181 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     3241 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     3301 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     3361 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     3421 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     3481 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     3541 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     3601 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     3661 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     3721 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     3781 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     3841 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     3901 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     3961 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     4021 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     4081 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     4141 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     4201 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     4261 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     4321 tcggagcgtg attataaaaa ctggttctgt tgcaaagttt taaatctact atcaaataa
//
LOCUS       DACCIQ010000289         3814 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.151, whole genome shotgun sequence.
ACCESSION   DACCIQ010000289 DACCIQ010000000
VERSION     DACCIQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.151"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            183..1250
                     /gene="rlmN"
                     /locus_tag="IVM62_002987"
     CDS             183..1250
                     /gene="rlmN"
                     /locus_tag="IVM62_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6846861.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1443..2546)
                     /locus_tag="IVM62_002988"
     CDS             complement(1443..2546)
                     /locus_tag="IVM62_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6846862.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3099..3647
                     /gene="aac(6')"
                     /locus_tag="IVM62_002989"
     CDS             3099..3647
                     /gene="aac(6')"
                     /locus_tag="IVM62_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6846863.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1247 a          648 c          764 g         1155 t
ORIGIN      
        1 atcaattctg attcatcagt cccataaatt atagagccat cttttctaat acggtatgat
       61 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
      121 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
      181 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
      241 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
      301 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
      361 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
      421 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
      481 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
      541 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
      601 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
      661 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
      721 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
      781 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
      841 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
      901 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
      961 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     1021 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     1081 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     1141 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     1201 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     1261 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     1321 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     1381 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     1441 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     1501 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     1561 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     1621 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     1681 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     1741 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     1801 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     1861 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     1921 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     1981 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     2041 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     2101 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     2161 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     2221 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     2281 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
     2341 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
     2401 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
     2461 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
     2521 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
     2581 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
     2641 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
     2701 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
     2761 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     2821 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     2881 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     2941 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     3001 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     3061 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     3121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     3181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     3241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     3301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     3361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     3421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     3481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     3541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     3601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     3661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     3721 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     3781 tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DACCIQ010000290         3666 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.154, whole genome shotgun sequence.
ACCESSION   DACCIQ010000290 DACCIQ010000000
VERSION     DACCIQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.154"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            594..1091
                     /gene="dfrG"
                     /locus_tag="IVM62_002990"
     CDS             594..1091
                     /gene="dfrG"
                     /locus_tag="IVM62_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP6846864.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1163..3115)
                     /locus_tag="IVM62_002991"
     CDS             complement(1163..3115)
                     /locus_tag="IVM62_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP6846865.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3122..3418)
                     /locus_tag="IVM62_002992"
     CDS             complement(3122..3418)
                     /locus_tag="IVM62_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6846866.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1123 a          629 c          595 g         1319 t
ORIGIN      
        1 atattctttc atctttttca aaaatacaca gagtatttat accactggag atctttcttt
       61 tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac
      121 attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc
      181 tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt
      241 attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa
      301 aaaagcggca tttttatttt aagtagttca gttttggagt acaaaatgac aaaatgtact
      361 tcaaaatgga ctactttttt cattttaact tacataccaa attacataca aaatgattta
      421 taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg attttgtggt attatgataa
      481 tataacttat agaaaaaatt gcttattcaa ctaacgggca ggataatgag aaaaggtttt
      541 ttgatagaaa taagaaatta gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag
      601 tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat gacattcctt
      661 ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat ccgataatat
      721 taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga aatattattc
      781 tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca atagaagatg
      841 tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa cagatttata
      901 atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat gaattcgaag
      961 gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt gcccaaaaag
     1021 ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa agaaaaaact
     1081 tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca caagagcttg
     1141 ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa tcaaattgtt
     1201 tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct gatttaaaag
     1261 ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca tacttcggat
     1321 tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat ggtctttcaa
     1381 gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg tcattaacat
     1441 ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa
     1501 ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta tcggcatatt
     1561 tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga cttgttagta
     1621 attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat cctctgatat
     1681 ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat tgttttctgc
     1741 ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt aattctttcg
     1801 tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc attcgccatt
     1861 cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta taaggagcat
     1921 caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat tcgtgaagat
     1981 aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa gaccaatcta
     2041 acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt cttgtatctt
     2101 catttaattc atccattgaa atattccaat cataggcaac atcagaacgg aacacatatc
     2161 ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct tcaaaccccg
     2221 tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag tgcatcttgt
     2281 ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt
     2341 ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag gttccaactc
     2401 caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc atcttagcga
     2461 acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct ctttttggta
     2521 gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg cccctacttt
     2581 tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct tccgagcagc
     2641 cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt tctataaggc
     2701 gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag ttttgcgata
     2761 cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta tctttggaac
     2821 taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg tgctgttctt
     2881 tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga tattcagaac
     2941 cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt
     3001 ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa
     3061 ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg gtcattgtct
     3121 atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta cttcgcttgt
     3181 tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat tcatccactg
     3241 cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc cttacttgct
     3301 ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga aattgcatag
     3361 gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca atttgcattc
     3421 ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat tttgtggtgt
     3481 aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca gttgcaattt
     3541 gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact acttattttt
     3601 atttagttgt ctctatattt tctctgctta tcggacagta gacatctact atcgttccgc
     3661 aattgc
//
LOCUS       DACCIQ010000291         2066 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.184, whole genome shotgun sequence.
ACCESSION   DACCIQ010000291 DACCIQ010000000
VERSION     DACCIQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.184"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            <1..843
                     /locus_tag="IVM62_002993"
     CDS             <1..843
                     /locus_tag="IVM62_002993"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6846867.1"
                     /translation="RFDLQKGFPLLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNH
                     IWDEWAFERYIKSPDYNGPDMTDFGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAET
                     YGDLGHIYGYQWRHWETKDGGFIDQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALP
                     PCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHT
                     LGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKA
                     PIAV"
     gene            863..1390
                     /locus_tag="IVM62_002994"
     CDS             863..1390
                     /locus_tag="IVM62_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6846868.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1453..>2066)
                     /locus_tag="IVM62_002995"
     CDS             complement(1453..>2066)
                     /locus_tag="IVM62_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6846869.1"
                     /translation="EVFNAVTHGIGTGLSIAGLVVLLVKGAHLHSSLHIVSYAIYGSM
                     MILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCLLSIRGWLGWTLFVL
                     IWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPLYHSLGMTGVILMVA
                     GGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT          707 a          393 c          421 g          545 t
ORIGIN      
        1 cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc ttttggattg
       61 atcaaaagcg aattgctttg gtttttaaaa ggagatacga atatccgcta tctattacaa
      121 aataaaaacc atatttggga tgaatgggca tttgaacgct acatcaaaag tccagactat
      181 aatggaccag atatgactga ttttggccat agatgtttga cagatcctga attcaatgaa
      241 gaatataaaa agcagagcaa gcttttttgc gagaagatcc taaccgatga ttcctttgct
      301 gaaacatacg gcgatcttgg acacatctac ggctatcaat ggcgtcactg ggaaacaaaa
      361 gacggtggct ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat
      421 tctagaagat tgatcgtttc tgcatggaat ccggaagatg ttcctagcat ggctttgcct
      481 ccttgtcata cgatgtttca attttatgtt caagagggac ggctaagctg ccaattgtat
      541 caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg
      601 actcacctaa ttgcacatga aacaggttta gcggttggcg aatttgttca cacattagga
      661 gatgctcatc tttatatcaa tcatatcgaa caaatgaaag aacaattgag ccgagaaata
      721 cgcgcatttc cgactttagt tttgaatcag gaaaagcagt cagtatttga ttttgagatg
      781 gaagatatcc aattagaagg atatgaccca catccggcga tcaaagctcc tatcgctgtt
      841 tagaatcaga aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt
      901 gattggaaaa gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca
      961 aatgacagaa gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg
     1021 tccgttgccg aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg
     1081 cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat
     1141 ctttttcatc gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt
     1201 ataccgtaca gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg
     1261 gtctgattgg tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc
     1321 gcatcaattt gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt
     1381 gacgatataa aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct
     1441 ttattttttt tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat
     1501 aacaaacaaa tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc
     1561 gcctatcgtg taagaaagcc cgcctgctac catcaaaata acaccagtca ttccaagaga
     1621 gtgataaaga ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt
     1681 agatactttt gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag
     1741 aatcgcaaac aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag
     1801 caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac
     1861 ttggaacacc ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa
     1921 taaaagaatc atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa
     1981 gtgggcacct tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc
     2041 atgggtgact gcattaaaca cttcat
//
LOCUS       DACCIQ010000292         1784 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.199, whole genome shotgun sequence.
ACCESSION   DACCIQ010000292 DACCIQ010000000
VERSION     DACCIQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.199"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            1..576
                     /locus_tag="IVM62_002996"
                     /pseudo
     CDS             1..576
                     /locus_tag="IVM62_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            609..1517
                     /locus_tag="IVM62_002997"
     CDS             609..1517
                     /locus_tag="IVM62_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP6846870.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1514..1762
                     /locus_tag="IVM62_002998"
     CDS             1514..1762
                     /locus_tag="IVM62_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP6846871.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          628 a          255 c          391 g          510 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct ctgtagtagg tgttgatatt
       61 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
      121 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
      181 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
      241 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
      301 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
      361 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
      421 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
      481 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
      541 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
      601 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
      661 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
      721 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
      781 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
      841 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
      901 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
      961 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     1021 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     1081 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     1141 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     1201 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     1261 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     1321 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     1381 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     1441 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     1501 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     1561 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     1621 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     1681 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     1741 tttaacccgt ggtgctagtt aattttaata tcctaaagaa tatc
//
LOCUS       DACCIQ010000293         1349 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.213, whole genome shotgun sequence.
ACCESSION   DACCIQ010000293 DACCIQ010000000
VERSION     DACCIQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.213"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            46..297
                     /locus_tag="IVM62_002999"
                     /pseudo
     CDS             46..297
                     /locus_tag="IVM62_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IVM62_003000"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IVM62_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6846872.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACCIQ010000294         1151 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3377
            SAMN15566659-rid10371373.guided.222, whole genome shotgun sequence.
ACCESSION   DACCIQ010000294 DACCIQ010000000
VERSION     DACCIQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15566659
            Sequence Read Archive: SRR12245535
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820582.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820582.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 21:59:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 35 of 64
            Pseudo Genes (internal stop)      :: 10 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15566659-rid10371373.guided.222"
                     /isolate="VRE3377"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="37.81 S 144.96 E"
                     /collection_date="2014"
                     /collected_by="Alfred Health"
     gene            41..124
                     /locus_tag="IVM62_003001"
     CDS             41..124
                     /locus_tag="IVM62_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP6846873.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            249..986
                     /gene="erm(B)"
                     /locus_tag="IVM62_003002"
     CDS             249..986
                     /gene="erm(B)"
                     /locus_tag="IVM62_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6846874.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          438 a          187 c          176 g          350 t
ORIGIN      
        1 tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat tccaaatgcg
       61 ttatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa
      121 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac
      181 taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat cacttcagga
      241 gtgataacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac
      301 tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa
      361 caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg
      421 aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca
      481 ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg
      541 ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc
      601 gtgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc
      661 accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc
      721 cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc
      781 ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg
      841 tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg
      901 ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt
      961 atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta
     1021 cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta ataactacca
     1081 attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat ctatttctta
     1141 tcgatacaaa t
//
